Liquid culture device for cordyceps militaris

Abstract

The invention relates to the technical field of mycelium culture, and discloses a cordyceps militaris liquid culture device, which comprises a protection cabin, a culture container, a ventilation filtering device and a stirrer, a linear driving mechanism is arranged below the culture container and drives the lifting execution group to drive the breeding part to lift; the breeding part is formed by connecting a bearing frame and a plurality of circumferentially-arranged springs with a breeding table, and the outer end of the breeding table is of a wedge-shaped structure; the stirrer rotates to form a culture solution vortex, the dissolved oxygen amount is increased, and hyphae are pushed to the wall face of the container; the breeding table extends out through the delivery window when the bearing frame ascends, and lossless harvesting and replacement of a solid base layer and sporocarp are achieved; according to the device, through mechanical automatic operation, the problems that hyphae are unstable in wall hanging and sporocarp is damaged by manual harvesting are effectively solved, and the culture success rate and the harvesting efficiency are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of mycelial culture technology, and more specifically to a liquid culture device for Cordyceps militaris. Background Technology

[0002] Cordyceps militaris, as a precious edible and medicinal fungus, has always been a focus of industrial research in terms of its artificial cultivation technology; liquid submersion culture has attracted much attention due to its short growth cycle and high efficiency; in particular, magnetic stirring culture technology has shown unique potential in the induction culture of Cordyceps militaris fruiting bodies.

[0003] The basic principle of this technology is as follows: In a conical flask containing liquid culture medium, a vortex is formed in the center of the culture medium by rotating a magnetic stirrer. This process not only significantly increases the dissolved oxygen content of the culture medium, but more importantly, the vortex effect can bring the mycelium to the bottle wall area above the liquid surface. The moist bottle wall provides a humid, nutrient-rich and well-ventilated microenvironment for the mycelium, allowing it to attach and spread, eventually forming a dense mycelial membrane. This mycelial membrane, which is completely exposed to the air, is equivalent to constructing an "artificial substrate surface" in liquid culture, providing the necessary physical support for the differentiation of fruiting bodies. When the mycelium matures and receives appropriate environmental induction (such as temperature difference stimulation, photoperiod, etc.), primordia can differentiate on this mycelial membrane and further develop into complete fruiting bodies.

[0004] However, this existing magnetic stirring wall-mounted culture method has obvious technical defects, which restricts its industrial application and the improvement of product quality: First, the growth state of mycelium attached to the wall is extremely unstable. During the stirring process, the attached mycelial membrane is easily detached due to the scouring of the liquid flow, physical vibration or its own weight, and re-mixed into the culture medium. The detached mycelium not only cannot continue to develop into fruiting bodies, but will also be repeatedly sheared by the stirrer, resulting in cell damage, reduced activity, and may cause deterioration of the culture medium components, ultimately leading to fruiting body culture failure or low yield. Secondly, there are significant drawbacks in the harvesting process. After the fruiting bodies have grown and formed on the bottle wall, they usually need to be manually peeled off with the help of tools such as tweezers or scrapers. This operation method is very easy to cause mechanical damage to the delicate Cordyceps militaris fruiting bodies, such as breakage, morphological damage or surface tissue damage, which seriously reduces the integrity and commercial value of the finished fruiting bodies. In addition, the harvesting process is cumbersome, inefficient and easy to introduce contamination by miscellaneous bacteria. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to overcome the problems of low success rate of fruiting body culture caused by unstable mycelial adhesion in the liquid culture of Cordyceps militaris and easy damage to the finished fruiting body during harvesting.

[0006] This invention provides the following technical solution: a liquid culture device for Cordyceps militaris, comprising a protective chamber, a culture container assembled inside the protective chamber, a ventilation and filtration device located on top of the culture container, and a stirrer installed on top of the protective chamber and extending axially into the culture container; the stirrer rotates under drive, forming a vortex of culture liquid inside the culture container, significantly increasing dissolved oxygen and pushing the mycelium to the bottle wall area above the liquid surface; a linear drive mechanism is provided below the culture container, with a lifting actuator group extending longitudinally into the culture container on its side; a propagation section is installed at the top of the lifting actuator group; the forward drive of the linear drive mechanism can drive the lifting actuator group to rise to control the lifting of the propagation section; the linear drive... The reverse drive of the mechanism can drive the lifting actuator to descend in order to control the settling of the propagation section. The propagation section includes a support frame that moves along the inner wall of the culture container and a propagation platform that is slidably set on the top of the support frame and arranged circumferentially. The propagation platform and the support frame are elastically connected by a spring. When the propagation platform is blocked by the inner wall of the culture container, multiple propagation platforms are circumferentially spliced ​​and aligned with the upper layer of the support frame. A delivery window for the propagation platform to extend is opened on the periphery of the culture container. The bottom of the outer end of the propagation platform is a wedge-shaped structure, which enables it to extend and retract smoothly during the lifting and lowering of the support frame. This allows the solid substrate and its grown complete fruiting bodies on the propagation platform to be delivered out of the culture container through the delivery window, enabling rapid sampling and replacement of the solid substrate.

[0007] Furthermore, the breeding platform includes a tray that is slidably connected to the top wall of the support frame. A wedge-shaped block is fixedly provided on the bottom wall of the outer side of the tray, and a receiving groove for accommodating a solid base layer is opened on its top. The longitudinal section of the wedge-shaped block is triangular, and its base is an inclined surface that slopes upward from the inside to the outside, thereby forming a wedge-shaped structure at the bottom of the outer end of the breeding platform.

[0008] Furthermore, a limit barrier is fixedly installed on the inner side of the top wall of the tray. When the breeding platform extends to its maximum position, it can be stopped by the top edge of the delivery window, thereby limiting the extreme extension position of the breeding platform under the action of spring force, preventing the bottom inflection point of the wedge block from crossing the delivery window and the tray from derailing from the surface of the support frame. The T-shaped slider at the bottom of the tray does not exceed the delivery window and still maintains a sliding engagement with the slot of the support frame.

[0009] Furthermore, the periphery of the supporting frame is provided with a recessed groove that is recessed towards the center. The groove and the wedge block are located in the same plane, and the innermost edge of the groove is elastically connected to the wedge block by a spring.

[0010] Furthermore, the outermost contour of the supporting frame maintains a sliding contact fit with the inner wall of the culture container; the propagation platform can slide inward under the obstruction of the inner wall of the culture container, so that multiple propagation platforms arranged circumferentially can be assembled and aligned with the upper layer of the supporting frame to form a complete ring structure; it can stop at the liquid surface, so that the solid base layer can receive the mycelium pushed by the vortex effect.

[0011] Furthermore, the outer side of the culture container is provided with an opening and closing control mechanism for closing or opening the delivery window. The opening and closing control mechanism includes a collar that slides around the culture container. Connecting arms are rigidly connected to both sides of the collar, and each connecting arm is fixedly connected to the telescopic shaft of the telescopic cylinder installed on the inner side wall of the protective chamber.

[0012] Furthermore, the linear drive mechanism includes a bidirectional lead screw arranged laterally and rotatably connected at its end to the side wall of the protective cabin. The surface of the bidirectional lead screw has mirror-symmetrical threads and is threaded with symmetrically arranged sliding parts. The end of the bidirectional lead screw is fixedly connected to the output end of a second motor installed on the outer wall of the protective cabin.

[0013] Furthermore, the lifting actuator is configured as two symmetrical groups, each group including an inclined guide rail that tilts upward from the inside out and slides in cooperation with the sliding member, an upper connecting rod fixed to the inclined side of the inclined guide rail and fixedly connected to the supporting frame after penetrating the bottom wall of the culture container, and a lower connecting rod located on the lower inclined side of the inclined guide rail.

[0014] Furthermore, the lower connecting rod includes a cylinder fixed to the bottom wall of the protective chamber, inside which a piston rod extending upward and fixed to the lower inclined side of the inclined guide rail is movably inserted; the piston rod consists of a plug rod connected to the inclined guide rail and a plug handle that slides and seals within the cylinder, and a variable liquid chamber is formed in the inner cavity of the cylinder above the plug handle; the side wall of the variable liquid chamber is respectively provided with a one-way inlet valve communicating with an external supply source and a one-way outlet valve communicating with the inside of the culture container.

[0015] Furthermore, the protective chamber is fixed with a horizontally arranged partition, which divides the internal space into upper and lower chambers. The upper chamber is used to accommodate culture containers. A positioning groove is fixed in the center of the top wall of the partition. The bottom end of the culture container can be embedded in the positioning groove and locked by a locking mechanism installed on the side of the positioning groove.

[0016] The technical effects and advantages of this invention are as follows: This invention utilizes symmetrical sliding members that slide axially towards each other, pushing the corresponding inclined guide rail upwards, thereby causing the upper connecting rod and the propagation section supported at its top to be lifted smoothly as a whole. When the delivery window is open, the propagation platform pops outwards under the elastic restoring force of the spring, ultimately removing the solid substrate and mature fruiting bodies as a whole. This structure effectively solves the problem of low fruiting body cultivation success rate caused by unstable mycelial adhesion and easy detachment in existing liquid culture, realizing mechanized and non-destructive harvesting operations, completely avoiding physical damage to fruiting bodies caused by manual operation, and significantly improving the integrity rate of finished products and overall production efficiency. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0018] Figure 2 For the present invention Figure 1 A schematic diagram of the remaining structure after removing the front and rear hatches.

[0019] Figure 3 For the present invention Figure 2 Schematic diagram of the structure at point A in the middle.

[0020] Figure 4 For the present invention Figure 2 Front view of the structure.

[0021] Figure 5 For the present invention Figure 4 A sectional diagram of the structure.

[0022] Figure 6 For the present invention Figure 5 A top-down view of the remaining structure after the protective cabin has been removed.

[0023] Figure 7 This is a schematic diagram of the linear drive mechanism, lifting and lowering actuator, reproduction section, and opening and closing control mechanism during the fruiting body cultivation stage of the present invention.

[0024] Figure 8 For the present invention Figure 7 Schematic diagram of the structure at point B.

[0025] Figure 9 For the present invention Figure 7 Schematic diagram of the linear drive mechanism and lifting actuator.

[0026] Figure 10 For the present invention Figure 7 Schematic diagram of the reproductive section.

[0027] Figure 11 For the present invention Figure 10 Another perspective on the structure of the reproductive part.

[0028] Figure 12 This is a schematic diagram of the linear drive mechanism, lifting and lowering actuator, breeding section, and opening and closing control mechanism of the present invention during the seed body harvesting stage.

[0029] Figure 13 For the present invention Figure 12 Schematic diagram of the structure at point C.

[0030] Figure 14 For the present invention Figure 12 A frontal view of the upper part of the structure.

[0031] Figure 15 For the present invention Figure 12 Schematic diagram of the reproductive section.

[0032] Figure 16 For the present invention Figure 15 Another perspective on the structure of the reproductive part.

[0033] Figure 17 For the present invention Figure 15 A disassembled diagram of the reproductive section structure.

[0034] The attached figures are labeled as follows: 1. Protective chamber; 11. Partition; 12. Positioning groove; 2. Culture container; 21. Delivery window; 22. Overflow valve; 23. Fastening flange; 3. Stirrer; 31. First motor; 32. Stirrer; 4. Linear drive mechanism; 41. Sliding component; 411. Threaded sleeve; 412. Guide roller; 42. Bidirectional lead screw; 43. Second motor; 5. Lifting actuator; 51. Inclined guide rail; 52. Upper connecting rod; 53. Lower connecting rod; 531. Cylinder 532. Piston rod; 533. One-way inlet valve; 534. One-way outlet valve; 6. Breeding section; 61. Support frame; 611. Groove; 612. Bracket; 62. Breeding table; 621. Tray; 622. Limiting fence; 623. Wedge block; 63. Spring; 7. Opening and closing control mechanism; 71. Collar; 72. Connecting arm; 73. Telescopic cylinder; 8. Ventilation and filtration device; 9. Locking mechanism; 91. Lock; 92. Fastening bolt; 10. Eccentric handle. Detailed Implementation

[0035] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. In addition, the forms of the various structures described in the following embodiments are merely illustrative. The liquid culture device for Cordyceps militaris involved in the present invention is not limited to the structures described in the following embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Example 1, refer to Figures 1 to 17As shown, this invention provides a liquid culture device for Cordyceps militaris, including a protective chamber 1, a culture container 2 assembled inside the protective chamber 1, a ventilation and filtration device 8 located on top of the culture container 2, and a stirrer 3 installed on top of the protective chamber 1 and extending axially into the culture container 2. The stirrer 3 rotates under drive, forming a vortex in the culture solution within the culture container 2, significantly increasing dissolved oxygen levels and pushing the mycelium towards the bottle wall area above the liquid surface. A linear drive mechanism 4 is provided below the culture container 2, with a lifting actuator 5 extending longitudinally into the culture container 2 on its side. A propagation section 6 is installed at the top of the lifting actuator 5. The forward drive of the linear drive mechanism 4 can drive the lifting actuator 5 upward to control the lifting of the propagation section 6, and the reverse drive of the linear drive mechanism 4 can drive... The lifting actuator 5 descends to control the settling of the propagation section 6; the propagation section 6 includes a support frame 61 that moves along the inner wall of the culture container 2 and a propagation platform 62 that is slidably disposed on the top of the support frame 61 and arranged circumferentially. The propagation platform 62 and the support frame 61 are elastically connected by a spring 63; when the propagation platform 62 is stopped by the inner wall of the culture container 2, multiple propagation platforms 62 are circumferentially spliced ​​and aligned with the upper layer of the support frame 61; a delivery window 21 is opened on the periphery of the culture container 2 for the propagation platform 62 to extend out. The bottom of the outer end of the propagation platform 62 is a wedge-shaped structure, which enables it to extend and retract smoothly during the lifting and lowering of the support frame 61, thereby delivering the solid substrate and its grown complete fruiting bodies on the propagation platform 62 out of the culture container 2 through the delivery window 21, realizing rapid sampling and replacement of the solid substrate; In this embodiment, it should be noted that the breeding platform 62 includes a tray 621 that is slidably connected to the top wall of the support frame 61 via a T-shaped slider and a slot structure. A wedge block 623 is fixedly provided on the outer bottom wall of the tray 621, and a receiving groove for accommodating a solid base layer is opened on its top. The longitudinal section of the wedge block 623 is triangular, and its base is an inclined surface that slopes upward from the inside to the outside, thereby forming a wedge structure at the bottom of the outer end of the breeding platform 62. The inner side of the top wall of the tray 621 is also fixedly provided with a limiting fence 622. When the propagation platform 62 extends to its maximum position, it is stopped by the top edge of the delivery window 21, thereby limiting the extreme extension position of the propagation platform 62 under the elastic force of the spring 63. This prevents the bottom inflection point of the wedge block 623 from crossing the delivery window 21 and the tray 621 from derailing from the surface of the support frame 61, thus avoiding the problem of failure to automatically reset. When the propagation platform 62 extends outward to its extreme position, the T-shaped slider at the bottom of the tray 621 does not exceed the delivery window 21 and still maintains a sliding engagement with the slot of the support frame 61, thereby avoiding interference of the T-shaped slider with the squeezing and pushing relationship between the wedge block 623 and the delivery window 21 or the collar 71. The height distance of the delivery window 21 is at least enough to ensure that the solid base layer and the fruiting bodies growing on it can be easily removed after the tray 621 and the wedge block 623 extend. The surface of the limiting fence 622 is provided with a mesh structure to allow mycelium to pass through smoothly. Among them, the porous substrate composed of solid base layers (such as rice, wheat, millet and other grains) provides a huge surface area and stable physical support for the attachment, colonization and spread of mycelium. The support frame 61 has a recessed groove 611 on its periphery, which is located in the same plane as the wedge block 623. The innermost edge of the groove 611 is elastically connected to the wedge block 623 by a spring 63. When the propagation section 6 rises to a position close to the open delivery window 21, the wedge block 623 gradually loses its blocking effect and moves outward under the elastic restoring force of the spring 63, thereby pushing the tray 621 out of the delivery window 21 and exposed to the outside of the culture container 2. The outermost contour of the support frame 61 maintains a sliding contact with the inner wall of the culture container 2. The propagation platform 62 can slide inward under the blocking effect of the inner wall of the culture container 2, so that multiple propagation platforms 62 arranged circumferentially can be assembled and aligned with the upper layer of the support frame 61 to form a complete ring structure and maintain a sliding contact with the inner wall of the culture container 2. It can stop at the liquid surface, so that the solid substrate can receive the mycelium pushed by the vortex effect. The outer side of the culture container 2 is provided with an opening and closing control mechanism 7 for closing or opening the delivery window 21. The opening and closing control mechanism 7 includes a collar 71 that slides around the culture container 2. Both sides of the collar 71 are rigidly connected to connecting arms 72. Each connecting arm 72 is fixedly connected to the telescopic shaft of the telescopic cylinder 73 installed on the inner side wall of the protective chamber 1. Through the lifting and lowering movement of the collar 71, the opening and closing control of the delivery window 21 can be realized, and radial squeezing and pushing action can be applied to the wedge block 623. The stirrer 3 includes a first motor 31 installed on the top of the protective chamber 1 and a stir bar 32 fixed to the output shaft of the first motor 31 and extending downward into the culture container 2. The rotation diameter of the stir bar 32 is smaller than the minimum inner diameter of the support frame 61 to avoid motion interference. The linear drive mechanism 4 includes a bidirectional lead screw 42 arranged laterally and rotatably connected at its end to the side wall of the protective chamber 1. The surface of the bidirectional lead screw 42 has mirror-symmetrical threads and symmetrically arranged sliding members 41 are threadedly sleeved on it. The end of the bidirectional lead screw 42 is fixedly connected to the output end of a second motor 43 installed on the outer wall of the protective chamber 1. By driving the bidirectional lead screw 42 to rotate forward / reverse by the second motor 43, the two sliding members 41 can move horizontally towards or away from each other along the bidirectional lead screw 42. The lifting actuator group 5 is set into two symmetrical groups. Each lifting actuator group 5 includes an inclined guide rail 51 that is inclined from the inside out and slides in cooperation with the sliding member 41, an upper connecting rod 52 that is fixed to the upper inclined side of the inclined guide rail 51 and fixed to the supporting frame 61 after penetrating the bottom wall of the culture container 2, and a lower connecting rod 53 that is located on the lower inclined side of the inclined guide rail 51. Based on the sliding cooperation between the sliding member 41 and the inclined guide rail 51 that is inclined from the inside out, the inclined guide rail 51 is adaptively pushed upward as the sliding member 41 moves inward, which drives the upper connecting rod 52 and the propagation part 6 to be vertically lifted as a whole. Among them, the innermost edge of the groove 611 extends radially to form a bracket 612 that is fixed to the top of the upper connecting rod 52. The bracket 612 is located in the gap between the adjacent springs 63, which effectively avoids spatial interference between the structures. A sealing structure is provided between the upper connecting rod 52 and the pre-set perforation on the bottom wall of the culture container 2, such as using a sealing ring or a sealing gasket to achieve the seal. The sliding member 41 includes a threaded sleeve 411 threaded through by the bidirectional lead screw 42 and a guide roller 412 fixed to the side wall of the threaded sleeve 411 and slidingly engaged with the inner wall of the inclined guide rail 51. The guide roller 412 effectively reduces the frictional resistance during the transmission conversion process between the sliding member 41 and the inclined guide rail 51, and reduces wear.

[0037] Example 2 is based on Example 1, with reference to... Figure 7 , Figure 9 as well as Figure 12 As shown, in order to maintain the supply balance of culture medium in culture container 2 after fruiting body harvesting and keep the total amount of culture medium relatively constant in each culture cycle, the structure of the lower connecting rod 53 needs to be further optimized: the lower connecting rod 53 includes a cylinder 531 fixed to the bottom wall of the protective chamber 1, and a piston rod 532 that extends upward and is fixed to the lower inclined side of the inclined guide rail 51 is movably inserted inside the cylinder 531; the piston rod 532 consists of a plug rod connected to the inclined guide rail 51 and a plug handle that slides and seals within the cylinder 531, with the cylinder handle above the plug handle. The inner cavity of body 531 forms a variable liquid chamber; the side wall of the variable liquid chamber is respectively provided with a one-way liquid inlet valve 533 connected to an external supply source, and a one-way liquid outlet valve 534 connected to the inside of the culture container 2; during the vertical lifting process, the inclined guide rail 51 drives the piston rod 532 to move upward, so that the plug handle at the bottom of the piston rod 532 compresses the variable liquid chamber in the cylinder body 531 upward, and introduces the culture medium in the variable liquid chamber into the culture container 2 through the one-way liquid outlet valve 534 and its connecting pipe, thereby replenishing the liquid inside; In this embodiment, it should be specifically noted that, referring to Figure 5As shown, an overflow valve 22 for controlling the culture medium level is installed on the side wall of the culture container 2. The overflow valve 22 is connected to an external recovery device. When the culture medium level is too high, excess liquid can automatically overflow through the overflow valve 22 and the connecting pipeline and be introduced into the recovery system, thereby effectively maintaining the dynamic supply balance of the culture medium in the culture container 2. (Note: The supply amount of the variable liquid chamber per cycle should be greater than the loss of culture mycelium). The telescopic structure formed by the cylinder 531 and the piston rod 532 not only meets the lifting requirements of the inclined guide rail 51 and the upper connecting rod 52, but also provides reliable foundation stability for the overall vertical movement.

[0038] Example 3 is based on Example 2, with reference to Figures 2 to 4 As shown, a horizontally arranged partition 11 is fixed inside the protective chamber 1, which divides the internal space into upper and lower chambers. The upper chamber is used to accommodate the culture container 2. A positioning groove 12 is fixedly provided in the center of the top wall of the partition 11. The bottom end of the culture container 2 can be embedded in the positioning groove 12 and locked by a locking mechanism 9 installed on the side of the positioning groove 12, thereby solving the problem of disassembling and cleaning the culture container 2. The locking mechanism 9 includes a latch 91 that is slidably connected to the upper side of the positioning groove 12. Its top end bends inward and covers the surface of the fastening flange 23 on the side wall of the culture container 2. The bottom edge of the latch 91 is equipped with a fastening bolt 92. By tightening the fastening bolt 92, the latch 91 can be pressed against the surface of the fastening flange 23, thereby achieving stable fixation of the culture container 2. The connection between the one-way drain valve 534 and the overflow valve 22 at the connection end on the side wall of the culture container 2 is detachable to avoid interference between the pipelines during disassembly. The upper connecting rod 52 is designed as a detachable two-section structure, with the two sections connected by a threaded connection. When disassembling, it is not necessary to disassemble the propagation section 6. Simply disconnect the upper connecting rod 52 to keep the propagation section 6 inside the culture container 2 and remove it together with it. The protective chamber 1 has doors on both the front and rear side walls for easy loading and unloading of objects. The top cover of the protective chamber 1 is embedded in a pre-set receiving groove on its top, and eccentric handles 10 are rotatably connected at the four corners. The top cover can be pressed and fixed by rotating the eccentric handles 10. The first motor 31 is installed on the top cover, and its output shaft is connected to the stir bar 32 through a coupling. The stir bar 32 passes through the ventilation filter device 8 and extends into the culture container 2 without interfering with the propagation section 6. The ventilation filter device 8 is closed on the top of the culture container 2. When disassembling, first disconnect the ventilation filter device 8 from the culture container 2, then loosen the eccentric handles 10 to remove the top cover and the stir bar 3 and ventilation filter device 8 together. Finally, after disconnecting the culture container 2 from the locking mechanism 9, the upper connecting rod 52 and related pipelines, the culture container 2 and the propagation section 6 inside can be pulled out from the top of the protective chamber 1 as a whole.

[0039] Working principle of this invention: During the fruiting body cultivation stage, the stir bar 32, which extends into the cultivation container 2, rotates under the drive of the first motor 31, forming a vortex in the center of the culture medium to significantly increase the dissolved oxygen content. This vortex effect can push the propagating hyphae to the bottle wall area above the liquid surface, allowing them to enter the multiple propagation platforms 62 that are circumferentially assembled into a ring, and finally fall onto the solid base layer laid on top of the tray 621. This solid base layer provides the hyphae with a moist, nutrient-rich, and well-ventilated microenvironment, which not only promotes stable adhesion and rapid spread of the hyphae, but also effectively avoids problems such as detachment, shear damage, decreased activity, and deterioration of the culture medium caused by unstable hyphae attachment. This significantly shortens the time from the differentiation of the hyphal membrane primordium to the formation of the fruiting body, achieving complete and efficient development of the fruiting body. During the fruiting body harvesting stage, after the solid base layer of tray 621 is covered with mature fruiting bodies, the second motor 43 is started to drive the bidirectional lead screw 42 to rotate clockwise. The two symmetrical sliding parts 41 with transmission threads on its surface slide horizontally towards each other and gradually converge towards the center. Based on the sliding cooperation between the sliding parts 41 and the inclined guide rail 51 that is inclined inward and outward, the inclined guide rail 51 is adaptively pushed upward as the sliding parts 41 move inward, which drives the upper connecting rod 52 fixed thereto and the propagation section 6 supported at the top of the upper connecting rod 52 to be vertically lifted as a whole. When the propagation section 6 rises close to the culture container When the delivery window 21 on the side wall is in position, the telescopic cylinder 73 retracts inward along its telescopic axis, and the connecting arm 72 pulls the collar 71 fixed to it to move down along the outer wall of the culture container 2 as a whole, so that the collar 71 is removed from the delivery window 21 and opens it; at this time, the wedge block 623 in the propagation table 62 gradually loses its stop and moves outward under the elastic restoring force of the spring 63, causing the entire propagation table 62 to gradually pop outward through the delivery window 21, so that the tray 621 is exposed to the outside of the culture container 2, making it convenient for the operator to take out the solid substrate and the complete fruiting bodies grown on it together and replace them; During the vertical lifting process, the inclined guide rail 51 simultaneously drives the piston rod 532 fixed at its bottom to move upward, causing the piston rod 532 to gradually be pulled out of the cylinder 531. At the same time, the plug at the bottom of the piston rod 532 compresses the variable liquid chamber in the cylinder 531 upward, and introduces the culture medium in the variable liquid chamber into the culture container 2 through the one-way drain valve 534 and its connecting pipe, thereby replenishing the liquid inside. If the culture medium level is too high, the excess liquid can overflow and be recovered through the overflow valve 22 and its connecting pipe. This structure effectively ensures the supply balance of culture medium in the culture container 2, so that the total amount of culture medium in each culture cycle is maintained within a relatively constant range. Conversely, after the solid substrate replacement is completed, the equipment is reset in the reverse order: First, the telescopic cylinder 73 extends outward, pushing the connecting arm 72 and the fixed collar 71 to move upward along the outer wall of the culture container 2. This causes the wedge block 623 to be squeezed inward by the collar 71 and compress the spring 63, driving the entire propagation platform 62 to gradually retract into the culture container 2 until the collar 71 re-closes the delivery window 21. Then, the second motor 43 drives the bidirectional lead screw 42 to rotate counterclockwise, driving the two sliding parts 41 to slide horizontally in opposite directions and separate to both sides. The inclined guide rail 51 slides down adaptively as the sliding parts 41 move outward, driving the upper connecting rod 52 and the propagation section 6 to descend vertically. At this time, the multiple propagation platforms 62 have completed circumferential splicing and alignment on the upper layer of the support frame 61, so that the propagation section 6 and the inner wall of the culture container 2 maintain sliding contact until the whole is restored to the starting position of the culture (the liquid level of the culture medium), preparing for the next culture cycle. During the equipment reset phase, the downward movement of the inclined guide rail 51 synchronously drives the piston rod 532 fixed at its bottom to descend, causing the piston rod 532 to gradually retract into the cylinder 531, thereby expanding the volume of the variable liquid chamber and forming a negative pressure. Then, the culture medium supplied from the external source can be drawn into the variable liquid chamber for storage through the one-way liquid inlet valve 533, preparing for the supply of culture medium to the culture container 2 in the next culture cycle.

[0040] The above is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, in accordance with the technical plan and its improved concept, should be included under the protection of the present invention.

Claims

1. A liquid culture device for Cordyceps militaris, comprising a protective cabin (1), a culture container (2) assembled in the protective cabin (1), a ventilation filter device (8) arranged at the top of the culture container (2), and a stirrer (3) installed at the top of the protective cabin (1) and axially extending into the culture container (2); the stirrer (3) rotates under the drive to form a culture liquid vortex in the culture container (2), significantly increasing the dissolved oxygen content and pushing the mycelium to the bottle wall area above the liquid surface, characterized in that: The linear drive mechanism (4) is arranged below the culture container (2), and a lifting execution group (5) extending into the culture container (2) is arranged on the side of the linear drive mechanism (4) in the longitudinal direction. The top end of the lifting execution group (5) is provided with a propagation part (6). The forward driving of the linear drive mechanism (4) can drive the lifting execution group (5) to rise, so as to control the lifting of the propagation part (6). The reverse driving of the linear drive mechanism (4) can drive the lifting execution group (5) to descend, so as to control the sinking of the propagation part (6). The propagation part (6) comprises a bearing frame (61) moving along the inner wall of the culture container (2) and a propagation table (62) slidingly arranged on the top of the bearing frame (61) and arranged in a circumferential direction. The propagation table (62) and the bearing frame (61) are elastically connected through a spring (63). When the propagation table (62) is blocked by the inner wall of the culture container (2), a plurality of propagation tables (62) are circumferentially combined and aligned on the upper layer of the bearing frame (61). A delivery window (21) is arranged on the side of the culture container (2) for the propagation table (62) to extend out. The bottom of the outer end of the propagation table (62) is provided with a wedge structure, so that the propagation table (62) can stably extend and retract during the lifting of the bearing frame (61). Therefore, the solid base layer and the complete fruiting body grown on the solid base layer can be delivered out of the culture container (2) through the delivery window (21), so as to realize rapid sampling and replacement of the solid base layer. ​ 2. The device for liquid culture of Cordyceps militaris according to claim 1, wherein: The propagation table (62) comprises a tray (621) slidingly connected to the top wall of the bearing frame (61). The bottom wall of the outer side of the tray (621) is fixedly provided with a wedge block (623). The top of the wedge block (623) is provided with a receiving groove for accommodating the solid base layer. The longitudinal section of the wedge block (623) is triangular. The bottom side is an inclined surface inclined outward and upward from the inside. Thus, the wedge structure of the bottom of the outer end of the propagation table (62) is formed.

3. The device for liquid culture of Cordyceps militaris according to claim 2, wherein: The inner side of the top wall of the tray (621) is fixedly provided with a limiting fence (622). When the propagation table (62) extends to the maximum position, the limiting fence (622) can be stopped by the top edge of the delivery window (21). Thus, the limit extension position of the propagation table (62) under the elastic force of the spring (63) is limited. The bottom end of the wedge block (623) is prevented from exceeding the delivery window (21) and the tray (621) from derailing from the surface of the bearing frame (61). The T-shaped sliding block at the bottom of the tray (621) does not exceed the delivery window (21) and still maintains the sliding fitting relationship with the clamping groove of the bearing frame (61).

4. The device for liquid culture of Cordyceps militaris according to claim 2, wherein: The circumferential side of the bearing frame (61) is provided with a groove (611) recessed towards the center. The groove (611) and the wedge block (623) are located in the same plane. The edge of the innermost side of the groove (611) and the wedge block (623) are elastically connected through the spring (63).

5. The device for liquid culture of Cordyceps militaris according to claim 4, wherein: The outermost contour of the bearing frame (61) is in contact with the inner wall of the culture container (2) and is slidingly fitted. The propagation table (62) can slide inwards under the stopping action of the inner wall of the culture container (2). Thus, a plurality of circumferentially arranged propagation tables (62) are combined and aligned on the upper layer of the bearing frame (61) to form a complete annular structure. The propagation table (62) can be stopped at the liquid level, so that the solid base layer can receive the mycelium pushed by the vortex effect.

6. The device for liquid culture of Cordyceps militaris according to claim 1 or 4, wherein: The culture container (2) is provided with an opening and closing control mechanism (7) for closing or opening a delivery window (21) on the outer side of the culture container (2), the opening and closing control mechanism (7) comprises a sleeve ring (71) sleeved on the lateral side of the culture container (2), and a connecting arm (72) is rigidly connected to the left and right sides of the sleeve ring (71), and each connecting arm (72) is fixedly connected with a telescopic shaft of a telescopic cylinder (73) installed on the inner side wall of the protection cabin (1).

7. The device for liquid culture of Cordyceps militaris according to claim 1, wherein: The linear drive mechanism (4) comprises a bidirectional screw rod (42) transversely arranged and rotatably connected at the end to the side wall of the protection cabin (1), the bidirectional screw rod (42) has mirror-symmetric screw threads on the surface and is threadedly sleeved with symmetrically arranged sliding members (41), and the end of the bidirectional screw rod (42) is fixedly connected with the output end of a second motor (43) installed on the outer wall of the protection cabin (1).

8. The device for liquid culture of Cordyceps militaris according to claim 7, wherein: The lifting execution group (5) is arranged in two groups symmetrically left and right, each group of the lifting execution group (5) comprises an inclined guide rail (51) inclined upward from inside to outside and slidably matched with the sliding member (41), an upper connecting rod (52) fixed to the upper inclined edge of the inclined guide rail (51) and fixedly connected with a bearing frame (61) after penetrating through the bottom wall of the culture container (2), and a lower connecting rod (53) arranged on the lower inclined edge of the inclined guide rail (51).

9. The device for liquid culture of Cordyceps militaris according to claim 8, wherein: The lower connecting rod (53) comprises a cylinder body (531) fixed to the bottom wall of the protection cabin (1), an upwardly extending piston rod (532) fixed to the lower inclined edge of the inclined guide rail (51) is movably inserted into the cylinder body (531); the piston rod (532) is composed of a plug rod connected with the inclined guide rail (51) and a plug handle sealingly sliding in the cylinder body (531), a variable liquid cavity is formed in the upper cavity of the cylinder body (531); the side wall of the variable liquid cavity is respectively provided with a one-way liquid inlet valve (533) in communication with an external supply source and a one-way liquid outlet valve (534) in communication with the inside of the culture container (2).

10. The device for liquid culture of Cordyceps militaris according to claim 1, characterized in that: The protection cabin (1) is fixedly provided with a transversely arranged partition plate (11) which divides the internal space into two chambers, the upper chamber is used for accommodating the culture container (2), a positioning groove (12) is fixedly arranged at the center of the top wall of the partition plate (11), the bottom end of the culture container (2) can be embedded into the positioning groove (12), and locking is realized through a locking mechanism (9) arranged on the side edge of the positioning groove (12).

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