Biological source organic water-soluble fertilizer and application thereof

Abstract

According to the biological source organic water-soluble fertilizer and the application thereof, the amino acid and the yeast source product are matched, the fertility is stable, the application effect is good, the effect of continuously providing the fertility can be achieved, and the biological source organic water-soluble fertilizer is beneficial to environmental development.

Description

Technical Field

[0001] This application relates to the field of agricultural technology, specifically to a bio-based organic water-soluble fertilizer and its uses. Background Technology

[0002] Traditional agricultural planting processes involve the extensive use of chemical fertilizers. However, the excessive use of chemical fertilizers can easily damage the natural nutrient structure of the soil, leading to soil compaction, poor soil nutrition, and hindering plant growth. This results in a significant reduction in crop yield and quality, and also contributes to the increasing deterioration of the natural environment.

[0003] In recent years, organic fertilizers have gradually become a research hotspot. Organic fertilizers offer more lasting fertility and contain a more comprehensive and abundant range of nutrients. Through microbial decomposition, they slowly and continuously supply plant growth, reducing the likelihood of soil compaction. Applying organic fertilizers can improve soil structure, coordinate water, fertilizer, air, and heat in the soil, and enhance soil fertility and land productivity. Currently, most commercially available organic fertilizers are simply prepared by mixing organic waste (such as fermentation waste liquid and residue) with other nutrients. On the one hand, organic waste undergoes numerous processing steps, resulting in complex compositions and difficult quality control, leading to unstable fertilizer fertility. On the other hand, some organic wastes contain large amounts of difficult-to-absorb organic matter, requiring specific soil microorganisms to enhance fertility, significantly increasing application costs. Therefore, there is still room for improvement in existing organic water-soluble fertilizer technologies. Summary of the Invention

[0004] The purpose of this invention is to overcome the problems existing in the prior art and provide a bio-based organic water-soluble fertilizer with stable fertility and good application effect. The organic water-soluble fertilizer uses a combination of amino acids and yeast-derived products, is rich in nutrients, can continuously provide fertility, and is beneficial to environmental development.

[0005] The first aspect of the present invention provides an organic water-soluble fertilizer, which comprises the following components: amino acids, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, phosphoric acid, and yeast-derived products.

[0006] In a preferred embodiment, the yeast-derived product comprises: 1 part yeast cell residue and 5-10 parts yeast fermentation broth.

[0007] In a preferred embodiment, the amino acid is an amino acid solution, specifically an amino acid stock solution composed of multiple amino acids, wherein the concentration of free amino acids is greater than or equal to 180 g / L; and the nitrogen content in the ammonium nitrate solution is greater than or equal to 400 g / L.

[0008] In a preferred embodiment, the mixture further includes an organic carbon source; the raw material for the organic carbon source is selected from one or more of starch, fruit peel, rice bran, corn flour, tapioca flour, pineapple pulp, and molasses. More preferably, the organic carbon source is obtained through fermentation and / or concentration.

[0009] In a preferred embodiment, the components, by weight percentage, are: 0-25% organic carbon source, 25%-50% amino acid solution, 15% ammonium nitrate solution, 5% potassium dihydrogen phosphate, 3% potassium sulfate, 0.5% phosphoric acid, and the remainder being yeast-derived products.

[0010] In a preferred embodiment, a preservative is also added, wherein the preservative comprises 0.2% by weight. In another preferred embodiment, the method for preparing the yeast cell residue is as follows:

[0011] Yeast cells were mixed with buffer solution and then crushed. The precipitate was then separated and dried to obtain the yeast cell residue.

[0012] The method for preparing the yeast fermentation broth is to inoculate yeast cells into a sterile liquid culture medium for cultivation, and after the cultivation is completed, separate and remove the yeast cells, and the remaining liquid is the yeast fermentation broth.

[0013] The second aspect of the present invention provides a method for preparing the bio-based organic water-soluble fertilizer described in the first aspect: the method involves weighing and mixing the components, and then stirring them thoroughly to obtain the bio-based organic water-soluble fertilizer.

[0014] A third aspect of this invention provides an application of the bio-based organic water-soluble fertilizer described in the first aspect, wherein the bio-based organic water-soluble fertilizer is used to promote plant growth. The plants include, but are not limited to: lettuce, chili peppers, garlic, cauliflower, cabbage, radish, loofah, asparagus, bok choy, cherry, strawberry, lychee, hyacinth, spider plant, holly, etc.

[0015] In a preferred embodiment, the bio-based organic water-soluble fertilizer is applied by spraying or drip irrigation after dilution, with a dilution ratio of 200-1000 times and a dosage of 0.5L-3L / mu. The preferred dilution ratio is 200-600 times, more preferably 200-500 times. The specific dosage can be 0.5L / mu, 1L / mu, or 3L / mu.

[0016] Compared with the prior art, the present invention has the following beneficial effects or advantages:

[0017] (1) The bio-source organic water-soluble fertilizer of the present invention uses a combination of amino acids and yeast products. Both main components are bio-source organic raw materials, which can provide rich organic nutrients. As they are by-products obtained through fermentation or enzymatic hydrolysis, the biomolecules are small and fast-acting. After application, they can take effect quickly and be absorbed and utilized by plants. After trial use, lettuce and peppers can show growth promotion effect within 10 days of fertilization.

[0018] (2) The bio-source organic water-soluble fertilizer of the present invention adds an amino acid solution. The amino acid solution is mainly obtained by fermentation or hydrolysis of protein raw materials. It is used in combination with the nitrogen source of yeast products, which has high biological activity. Other small molecule nitrogen, phosphorus and potassium components are also added. The combination of macromolecules and small molecules improves the utilization rate of nutrients. The resulting water-soluble fertilizer is nutritionally comprehensive and balanced, thereby improving soil fertility.

[0019] (3) The bio-source organic water-soluble fertilizer of the present invention provides rich nutrients for microorganisms in the soil, improves their biological activity, promotes the decomposition and release of organic components, can provide fertilizer for a long time, and will not cause soil compaction after application, thus improving soil structure.

[0020] (4) The yeast-derived product used in the bio-based organic water-soluble fertilizer of this invention consists of mother fermentation broth and yeast cell residue. Both are byproducts of yeast extract preparation. The research and development of in vitro protein synthesis technology based on yeast cells requires cultivating large quantities of yeast and obtaining yeast cell lysates. However, the fermentation broth after yeast fermentation and the remaining cell components after extraction of soluble substances from yeast cells often contain a large amount of beneficial substances that cannot be effectively utilized. By preparing bio-based organic water-soluble fertilizer, byproducts or waste from other production lines are fully utilized, effectively utilizing waste resources and reducing environmental pollution. Furthermore, the preparation method is simple and easy to operate, further reducing production difficulty and cost.

[0021] It should be understood that, within the scope of this invention, the above-described technical features of this invention and the technical features specifically described below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be described in detail here. Detailed Implementation

[0022] This invention, based on extensive and in-depth research, and through numerous screenings and trials, proposes a bio-based organic water-soluble fertilizer with stable fertility and good application effects. The organic water-soluble fertilizer uses a combination of amino acids and yeast-derived products, while also adding small-molecule nitrogen, phosphorus, and potassium components, resulting in a rich overall nutritional profile that provides sustained fertility and is beneficial to environmental development.

[0023] The present invention will be further illustrated below with reference to specific embodiments and examples. For the specific methods or materials used in the examples, those skilled in the art can make conventional substitutions based on the technical concept of the present invention and existing technologies, and are not limited to the specific descriptions in the embodiments of the present invention.

[0024] The present invention discloses a bio-based organic water-soluble fertilizer, specifically comprising the following components: amino acids, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, phosphoric acid, and yeast-derived products. The amino acids refer to a mixture of amino acids, used to provide nitrogen-containing small organic molecules. The yeast-derived products are a highly absorbable organic nitrogen source. Ammonium nitrate, potassium sulfate, and phosphoric acid further supplement the small-molecule nitrogen, phosphorus, and potassium components, resulting in a balanced overall nutritional profile of the organic water-soluble fertilizer. Furthermore, the combination of large and small molecules ensures good absorption and long-lasting fertilizing power.

[0025] In one example, the yeast-derived product includes: 1 part yeast cell residue and 5-10 parts yeast fermentation broth.

[0026] The yeast cell residue and yeast fermentation broth mentioned above are byproducts or waste products obtained by the applicant during the process of cell-free protein synthesis. The applicant has found through research that the yeast cell residue and yeast fermentation broth are rich in nutrients and organic acids, which have a neutralizing effect on soil salinity and alkalinity. Therefore, based on this, the applicant developed the organic water-soluble fertilizer of this application.

[0027] In one example, the amino acid is an amino acid solution, specifically an amino acid stock solution composed of multiple amino acids, wherein the concentration of free amino acids is greater than or equal to 180 g / L; the nitrogen content of the ammonium nitrate solution is greater than or equal to 400 g / L. The amino acid refers to a composition containing multiple amino acids essential for plant growth. In a preferred example, a liquid form of amino acids, i.e., an amino acid stock solution, is used, which is more easily absorbed. The amino acid stock solution can be self-made, such as using protein-containing raw materials of animal or plant origin, which undergo enzymatic hydrolysis, acid hydrolysis, microbial fermentation, etc., followed by solid-liquid separation to obtain a liquid containing amino acids; alternatively, it can be directly purchased from commercially available amino acid stock solution products as raw materials. The concentration of free amino acids in the amino acid solution must be greater than or equal to 180 g / L to ensure sufficient easily absorbed amino acid components. The ammonium nitrate solution provides an easily absorbed small-molecule nitrogen source, which complements the large-molecule nitrogen source in the yeast-derived product to maintain long-lasting fertility.

[0028] In one example, an organic carbon source is also included; this organic carbon source is obtained through fermentation and / or concentration. The raw materials for the organic carbon source are selected from one or more of starch, fruit peels, rice bran, corn flour, cassava flour, pineapple pulp, and molasses. Organic carbon sources refer to organic substances that can provide the carbon elements needed for the growth and reproduction of microorganisms, such as glucose, fructose, and starch. Waste or by-products rich in organic carbon sources are frequently found in the food industry or biotechnology sector. These wastes or by-products serve as excellent energy sources in fertilizers. Furthermore, because the composition of waste is often complex, the organic carbon source obtained through fermentation, in addition to being rich in energy, also contains nutrients such as protein, macro-elements, and micro-elements, all of which can provide nutrition for plants. Fermented organic carbon sources further degrade macromolecules and, under the action of microorganisms, obtain more secondary metabolites, further improving the plant's absorbability. The fermented organic carbon source can also be added in the form of a fermentation concentrate, such as molasses fermentation concentrate, starch fermentation concentrate, cassava fermentation concentrate, etc., with molasses fermentation concentrate being preferred.

[0029] In one example, the components, by weight percentage, are: 0-25% organic carbon source, 25%-50% amino acid solution, 15% ammonium nitrate solution, 5% potassium dihydrogen phosphate, 3% potassium sulfate, 0.5% phosphoric acid, and the remainder being yeast-derived products. Preferably, the sum of the organic carbon source and the amino acid solution is 50%, meaning the amount of molasses can be 0, in which case the percentage of amino acid solution is 50%.

[0030] In another example, a preservative is also added, comprising 0.2% by weight. The purpose of adding the preservative is to further extend the shelf life and usability of the organic water-soluble fertilizer; therefore, there are no strict limitations on the specific type, as long as it is suitable for use in organic fertilizers. Common examples include benzalkonium chloride.

[0031] In one example, the method for preparing the yeast cell residue is as follows: yeast cells are mixed with a buffer solution and then crushed, followed by separation to obtain a precipitate, which is then dried to obtain the yeast cell residue. The yeast cell residue is the cell component remaining after the extraction of soluble substances from yeast cells. The yeast cell residue contains not only yeast cell wall fragments but also some fermentation products and intracellular metabolites, making it nutritious and rich in polysaccharides.

[0032] The method for preparing the yeast fermentation broth involves inoculating yeast cells into a sterile liquid culture medium for cultivation. After cultivation, the yeast cells are separated and removed, and the remaining liquid is the yeast fermentation broth. In short, the yeast fermentation broth refers to the liquid portion after yeast fermentation and removal of the yeast cells.

[0033] The sterile liquid culture medium comprises the following components and contents: glucose 20-50 g / L, sucrose 20-50 g / L, potassium dihydrogen phosphate 1-5 g / L, dipotassium hydrogen phosphate 1-5 g / L, magnesium sulfate heptahydrate 1-4 g / L, vitamin B1 5-20 g / L, and yeast extract 1-5 g / L; the inoculum amount of yeast in the sterile liquid culture medium is 5%-10% (v / v); the culture process is: cultured at 28-32℃ for 48-60 h; the separation is by centrifugation.

[0034] The yeast mentioned includes one or more of Kluyveromyces, Saccharomyces cerevisiae, or Pichia pastoris; the Kluyveromyces mentioned includes one or more of Kluyveromyces lactis, Kluyveromyces marx, Kluyveromyces dob, Kluyveromyces sacchariformis, Kluyveromyces wickheim, Kluyveromyces brittlewall, Kluyveromyces hubeiensis, Kluyveromyces multisporum, Kluyveromyces siamensis, or Kluyveromyces yarbromentosus.

[0035] In another example, the present invention also provides a method for preparing the bio-based organic water-soluble fertilizer described in the first aspect: the method involves weighing and mixing the components, and then stirring them thoroughly to obtain the bio-based organic water-soluble fertilizer.

[0036] In another example, the present invention also provides an application of the bio-based organic water-soluble fertilizer described in the first aspect, which is used to promote plant growth. The plants include, but are not limited to: lettuce, chili peppers, garlic, cauliflower, cabbage, radish, loofah, asparagus, bok choy, cherries, strawberries, lychees, hyacinths, spider plants, holly, etc.

[0037] In a preferred embodiment, the bio-based organic water-soluble fertilizer is applied by spraying or drip irrigation after dilution, with a dilution ratio of 200-1000 times and a dosage of 0.5L-3L / acre. The preferred dilution ratio is 200-600 times, more preferably 200-500 times. The specific dosage can be 0.5L / acre, 1L / acre, or 3L / acre. The application method can be spraying or drip irrigation onto the plant leaves or roots, and can be adjusted according to the characteristics of the plant.

[0038] Preparation of bio-derived organic water-soluble fertilizers in Examples 1 and 2

[0039] (1) Preparation of yeast-derived products

[0040] 1) Preparation of yeast cell residue: Yeast cells and buffer solution were mixed at a weight ratio of 1:1, then homogenized, centrifuged to obtain the precipitate, and then spray-dried to obtain yeast cell residue. The buffer solution was TE buffer solution.

[0041] 2) Preparation of yeast fermentation broth: Yeast cells were inoculated into a sterile liquid culture medium and then cultured at 30℃ for 52 hours. After the culture was completed, the yeast cells were removed by centrifugation, and the remaining liquid was the yeast fermentation broth. The sterile liquid culture medium contained the following components and concentrations: glucose 35 g / L, sucrose 35 g / L, potassium dihydrogen phosphate 3 g / L, dipotassium hydrogen phosphate 3 g / L, magnesium sulfate heptahydrate 2 g / L, vitamin B1 8 g / L, and yeast extract 3 g / L; the inoculum size of yeast cells in the sterile liquid culture medium was 7%.

[0042] 3) Preparation of yeast-derived products: Mix yeast cell residue and yeast fermentation broth in a ratio of 1:7 by weight. When mixing, add the yeast cell residue to the yeast fermentation broth in multiple batches to obtain a mixture. Stir the mixture until it is in suspension to obtain the yeast-derived products.

[0043] (2) Prepare bio-based organic water-soluble fertilizer

[0044] Taking the preparation of 100L of bio-based organic water-soluble fertilizer as an example, the raw materials and dosages are prepared according to the table in Table 1 below. Except for the yeast source product, all other raw materials are commercially available. The amino acid solution is an amino acid stock solution purchased from Xuzhou Green Source Agricultural Technology Co., Ltd., with a free amino acid content of 191g / L and a total nitrogen content of 94.3g / L; the ammonium nitrate solution contains 422g / L of nitrogen; the organic carbon source is molasses, specifically a concentrated fermented molasses solution; and the preservative used is benzalkonium chloride.

[0045] The specific preparation method of organic water-soluble fertilizer is as follows: weigh each component according to the dosage in Table 1, mix them in a mixing tank, and obtain the bio-source organic water-soluble fertilizer after thorough stirring. Store it in the tank for later use, as Example 1 and Example 2 respectively.

[0046] Table 1. Organic Water-Soluble Fertilizer Ingredient List

[0047]

[0048] The beneficial effects of the present invention will be further illustrated below with experimental data.

[0049] Experiment with a single vegetable

[0050] Test materials

[0051] 1. Materials and Methods:

[0052] 1.1 Experimental location: Vegetable greenhouse of Shanghai Xuanbao Fruit and Vegetable Professional Cooperative, Chongming District, Shanghai. The experimental crop was lettuce.

[0053] 1.2 Experimental tests: lettuce leaf width, leaf length, single plant weight, root length, etc.

[0054] 1.3 Test materials: Bio-derived organic water-soluble fertilizer prepared in Example 1, high-nitrogen fertilizer (liquid organic fertilizer, used as a basic control group), and commercially available protein organic fertilizer (Zhouyu fish protein organic fertilizer, powder).

[0055] 1.4 Experimental Design:

[0056] (1) The experimental land was evenly divided into 5 plots of the same size, each plot being 0.2 mu. Lettuce seeds were sown on each plot. After the seedlings emerged, thinning was carried out to leave lettuce seedlings of similar size. Finally, 24 lettuce plants were retained per square meter. Fertilization experiments were conducted at this density.

[0057] (2) The experimental vegetable plots were marked according to the numbers shown in Table 2 below, and fertilized according to the fertilization plan described in the table. Considering that the growth period of lettuce is relatively short, it was fertilized twice, with an interval of 10 days between the two applications. According to the growth needs of lettuce, high-nitrogen fertilizer was used as the base fertilizer. The specific fertilization methods were as follows: for group T1, high-nitrogen fertilizer and bio-derived organic water-soluble fertilizer were mixed according to the preset dosage and sprayed on lettuce; for group CK1, sprayed; for group CK2, diluted and dissolved with water and then sprayed.

[0058] Table 2. Experimental groupings and fertilization of lettuce.

[0059]

[0060]

[0061] (3) Ten days after the first fertilization, measure the width and length of the lettuce leaves. After the second fertilization, before the lettuce is harvested, measure and record the width, length, root length and weight of each plant.

[0062] 2. Experimental Results

[0063] The measurement data of leaf width and leaf length after the first fertilization are shown in Table 3 below. It can be seen that after the first fertilization, compared to the single high-nitrogen fertilizer group (CK1), the leaf length of experimental group T1 was significantly increased, and the leaf width of all leaves also increased significantly. This means that the bio-based organic water-soluble fertilizer of Example 1, at application rates of 0.5 L / mu, 1 L / mu, and 3 L / mu, can rapidly promote lettuce leaf growth. Furthermore, comparing group T1 with group CK2, it can also be seen that group T1 showed a significantly greater increase in leaf length than CK2, indicating that the bio-based organic water-soluble fertilizer of this invention has a better fertilization effect than commercially available organic fertilizers.

[0064] Table 3 Measurement results after the first fertilization

[0065]

[0066] The measurement data of leaf width, leaf length, root length, and single plant weight after the second fertilization are shown in Table 4 below. The data shows that the lettuce plants grew significantly in size after two topdressings. Compared with the CK1 group, which received only high-nitrogen fertilizer, the single plant weight of all plants in the T1 group was significantly higher. The leaf length and root length of all T1 groups were also significantly higher than those receiving only high-nitrogen fertilizer. In terms of leaf width, T1-2 and T1-3 showed significantly higher values ​​than the high-nitrogen fertilizer-only groups. Considering that leaf width is not the most important quality factor for lettuce, the overall effect of the T1 group was quite satisfactory.

[0067] Compared to the high-nitrogen fertilizer and commercially available organic fertilizer group CK2, group T1 showed significant improvements in single plant weight, leaf width, and root length. Furthermore, soil observation revealed that the soil treated with the bio-derived organic water-soluble fertilizer of this invention was loose and less prone to clumping.

[0068] Table 4 Measurement results after the second fertilization

[0069]

[0070] In summary, the bio-based organic water-soluble fertilizer of the present invention has better fertility and stability than commercially available organic fertilizers, and its application in combination with high-nitrogen fertilizers can significantly improve the growth rate and yield of lettuce.

[0071] Experiment 2: Chili Pepper Experiment (Thin-skinned Green Pepper)

[0072] Test materials

[0073] 1. Materials and Methods:

[0074] 1.1 Experimental site: Vegetable greenhouse of Shanghai Xuanbao Fruit and Vegetable Professional Cooperative, Chongming District, Shanghai. The experimental crop was thin-skinned green pepper.

[0075] 1.2 Experimental tests: chili plant height, number of fruits, weight of a single fruit, yield, fruit length and diameter, etc.

[0076] 1.3 Test materials: Bio-derived organic water-soluble fertilizer and high-nitrogen fertilizer (liquid organic fertilizer, used as a basic control group) prepared in Examples 1 and 2.

[0077] 1.4 Experimental Design:

[0078] (1) Divide the experimental land into 7 plots of the same size and plant the same batch of chili seedlings with similar height. The experiment will begin after the seedlings are confirmed to have survived.

[0079] (2) The seven experimental vegetable plots were marked with the numbers shown in Table 5 below, and fertilized according to the fertilization plan in the table. Fertilizer was applied three times, with an interval of 20 days between each application, i.e., on the 1st, 21st and 41st days.

[0080] Table 5. Experimental groups and fertilization of thin-skinned green peppers.

[0081]

[0082]

[0083] (3) Every 10 days after the first fertilization, the plant height of each group of thin-skinned green peppers was measured and recorded. That is, the plant height was measured on the 11th, 21st, 31st, 41st and 51st days. After the thin-skinned green peppers flowered, fruited and matured, the number of thin-skinned green pepper plants was counted. Five thin-skinned green pepper plants were randomly selected from each plot. The total number of peppers, total weight (i.e., sample weight), length and diameter of a single fruit and weight of a single fruit were measured on the five pepper plants. The total yield of peppers in each plot was calculated.

[0084] 2. Experimental Results

[0085] The specific experimental results regarding the plant height and yield of thin-skinned green peppers are shown in Tables 6 and 7 below.

[0086] As can be seen from the plant height data in Table 6, 30 days after fertilization, the plant height of the thin-skinned green peppers in both experimental groups was significantly higher than that in the control group. Furthermore, under appropriate application rates (e.g., 1-3 L / acre), rapid growth was observed shortly after fertilization. This demonstrates that the bio-based organic water-soluble fertilizer of this invention can promote plant growth.

[0087] As can be seen from the results in Table 7, after applying the bio-based organic water-soluble fertilizers of Examples 1 and 2, the length of the thin-skinned green peppers decreased compared to the control group, while the diameter increased significantly, and the single weight of the fruit increased significantly, with a relatively significant overall effect. Under appropriate dosage (e.g., 1-3 L / mu), the number of fruits and the overall yield increased significantly, indicating that the bio-based organic water-soluble fertilizer of the present invention can improve the yield and quantity of thin-skinned green peppers.

[0088] Furthermore, observation of the soil conditions reveals that the soil treated with the bio-based organic water-soluble fertilizer of this invention is loose and does not easily clump together.

[0089] Table 6. Results of thin-skinned green pepper growth

[0090]

[0091]

[0092] Note: The growth rates mentioned in the table are all relative to the growth rate of the control group CK3.

[0093] Table 7 Results of yield and quality of thin-skinned green peppers

[0094]

[0095] Note: The growth rates mentioned in the table are all relative to the growth rate of the control group CK3.

[0096] The above are only some embodiments of the present invention, and the present invention is not limited to the contents of the above embodiments.

[0097] All documents mentioned in this invention are incorporated herein by reference as if each document were individually incorporated by reference. Furthermore, it should be understood that after reading the foregoing description of this invention, those skilled in the art can make various alterations or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A bio-based organic water-soluble fertilizer, characterized in that, The organic water-soluble fertilizer comprises the following components: amino acid, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, phosphoric acid and yeast-derived product. The yeast-derived product comprises 1 part of yeast cell residue and 5-10 parts of yeast fermentation liquor.

2. The bio-source organic water-soluble fertilizer according to claim 1, characterized in that, The amino acid is an amino acid solution, specifically an amino acid stock solution composed of multiple amino acids, wherein the concentration of free amino acid is greater than or equal to 180 g / L; the nitrogen content in the ammonium nitrate solution is greater than or equal to 400 g / L.

3. The bio-source organic water-soluble fertilizer according to claim 1 or 2, characterized in that, The organic carbon source is selected from one or more of starch, fruit peel, rice bran, corn powder, cassava powder, pineapple residue and molasses.

4. The bio-source organic water-soluble fertilizer according to claim 3, characterized in that, The components are respectively 0-25% of organic carbon source, 25%-50% of amino acid solution, 15% of ammonium nitrate solution, 5% of potassium dihydrogen phosphate, 3% of potassium sulfate, 0.5% of phosphoric acid and the rest of yeast-derived product, in terms of weight percentage.

5. The bio-source organic water-soluble fertilizer according to any one of claims 2-4, characterized in that, The preparation method of the yeast cell residue is as follows: The yeast cell is mixed with buffer solution and then broken, and then the precipitate is separated and dried to obtain the yeast cell residue. The preparation method of the yeast fermentation liquor is as follows:

6. The method for preparing the bio-source organic water-soluble fertilizer according to any one of claims 1-5, characterized in that: The yeast cell is inoculated in a sterile liquid medium for culture, and then the yeast cell is removed after the culture to obtain the yeast fermentation liquor.

7. The use of the bio-organic water-soluble fertilizer according to any one of claims 1 to 5, characterized in that, The method is to mix the components after weighing, and then obtain the organic water-soluble fertilizer after sufficient stirring.

8. Use according to claim 7, characterized in that, The biological source organic water-soluble fertilizer is used for promoting the growth of plants. The use method of the biological source organic water-soluble fertilizer is to spray or drip irrigation after dilution, the dilution multiple is 200-1000, and the use amount after dilution is 0.5-3 L per mu. The use method of the biological source organic water-soluble fertilizer is to spray or drip irrigation after dilution, the dilution multiple is 200-1000, and the use amount after dilution is 0.5-3 L per mu.

Images