Microbiological detection device and detection method for antibacterial peptide
By designing a cylindrical testing device, efficient testing and sample addition of multiple test pieces were achieved, solving the problems of low testing efficiency and contamination in existing technologies and improving the reliability of test results.
Patent Information
- Application Number
- CN202511643767.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-11
- Publication Date
- 2026-03-06
AI Technical Summary
Existing antimicrobial peptide microbial detection devices can only hold one test strip at a time, resulting in low detection efficiency. Furthermore, the sample addition process is susceptible to environmental contamination, which can affect the accuracy of the test results.
A cylindrical testing device was designed, comprising a rotatable mounting base and a rotation positioning mechanism, capable of simultaneously carrying multiple test pieces, and achieving efficient and accurate sample addition through a sealing film and a sample addition through-hole.
It improves detection efficiency, prevents contamination of test strips during storage and sample loading, and ensures the reliability and accuracy of test results.
Smart Images
Figure CN121613097A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of test strip technology, and in particular to a microbial detection device and method for antimicrobial peptides. Background Technology
[0002] Antimicrobial peptides are an important class of active substances produced by the immune system of organisms. Rapid and convenient detection of antimicrobial peptides produced by microorganisms is of great significance for microbial function research, drug screening and food safety monitoring.
[0003] Immunochromatographic test strips (or test strips) offer the possibility of rapid detection. However, in practical applications, especially when high-throughput testing of multiple samples is required, existing testing devices can only handle one test strip at a time, resulting in low detection efficiency and failing to meet the needs of rapid screening of batches of samples. Secondly, the sample addition process is often an open operation, making test strips susceptible to environmental contamination during storage and addition, which affects the accuracy of the test results. Summary of the Invention
[0004] The purpose of this invention is to provide a microbial detection device and method for antimicrobial peptides, which improves the detection efficiency of test strips.
[0005] In a first aspect, a microbial detection device for an antimicrobial peptide includes: A cylindrical container, wherein the cylindrical container has an observation window and a sample feeding through hole on its wall; A rotatable mounting base is disposed inside the cylinder, and the mounting base is configured with a mounting station for mounting multiple test pieces; A rotary positioning mechanism is used to drive the mounting base to rotate and switch and position it between multiple installation stations; A cover is provided, which screws onto one end of the cylinder; A fixed shell, which screws onto the other end of the cylinder; The rotary positioning mechanism is configured to drive the mounting base to rotate, so that any of the test pieces mounted thereon enters the testing station, and aligns the sample inlet on the test piece with the sample dispensing through hole on the cylinder.
[0006] Preferably, the test strip includes: A backplate, wherein a sample pad, a conjugate pad, a reaction membrane carrying antimicrobial peptide specific recognition molecules and an absorbent pad are sequentially arranged and partially overlapped in the horizontal direction of the backplate; A protective shell is fixed to the top surface of the back plate and covers the aforementioned padding layers. The top surface of the protective shell is provided with a transparent observation plate. The sample inlet tube is disposed on the protective shell and located directly above the sample pad, and the top of the sample inlet tube is covered with a puncture-resistant sealing film. The injection tube constitutes the injection port.
[0007] Preferably, when the test piece is in the testing station, the dropper inserted through the sample dispensing hole can pierce the sealing film and dispense the sample.
[0008] Preferably, the rotary positioning mechanism includes: A rotating rod is located at one end of the mounting base facing the fixed shell. The fixed shell has a circular hole that mates with the rotating rod. The rotating rod is rotatably supported in the circular hole, and its outer circumference is evenly distributed with multiple slots. A locking element, which is disposed on the fixed housing and includes a pin and an elastic component; The pin engages with the slot under the action of the elastic component to lock the rotational position of the mounting base.
[0009] Preferably, the center of the bearing cover is hinged with a locking pin, and the mounting base has a groove at one end corresponding to the locking pin. The engagement of the locking pin with the groove achieves axial positioning of the mounting base.
[0010] Preferably, the locking element further includes a pull rod and a pull ring; The bottom end of the pull rod is integrally formed with the pin, and the top end of the pull rod passes through the fixing shell and is fixed with the pull ring; By pulling the pull ring, the elastic force of the elastic component can be overcome, causing the pin to disengage from the slot.
[0011] Preferably, the elastic component is a spring sleeved on the pull rod.
[0012] Preferably, the mounting base is rectangular, with inverted T-shaped grooves on all four sides. The back plate of the test piece can be inserted into the inverted T-shaped grooves, and the two sides of the back plate are symmetrically provided with springs for locking with the inverted T-shaped grooves.
[0013] Secondly, the present invention also provides a method for the microbial detection of antimicrobial peptides, using the above-mentioned detection device, comprising the following steps: Operate the rotary positioning mechanism to rotate the mounting base so that a test piece enters the testing station and locks in place; A sample containing antimicrobial peptides is added to the test piece at the testing station through the sample dispensing orifice. The presence of antimicrobial peptides is determined by reading signals through the observation window.
[0014] Preferably, the volume of the sample added is 50-200 μL, and the signal reading time is 5-15 min after sample addition.
[0015] The microbial detection device and method for antimicrobial peptides provided by this invention have the following beneficial effects: 1. Each pad of the test strip is placed in the protective shell, and the injection tube is covered with a sealing film to fully wrap the test strip and prevent it from being contaminated during storage and use; 2. The rectangular mounting base can carry four test pieces at a time. Under the action of the rotating rod, the rectangular mounting base rotates 90 degrees in the cylinder to the observation position for testing, which greatly improves the testing efficiency. 3. The design of the through hole and sample inlet tube of the cylinder allows for direct insertion of a quantitative dropper for sample addition, which is simple to operate and ensures the accuracy of the sample addition, thus improving the reliability of the test results. Attached Figure Description
[0016] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.
[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is an assembly drawing of the cylinder, observation window, sample feeding through hole, bearing cap, and fixing shell in this invention; Figure 3 This is an assembly diagram of the back plate, protective shell, observation plate, sample inlet tube, and sealing film in this invention; Figure 4 This is an exploded view of the backplate, sample pad, conjugate pad, reaction membrane, absorbent pad, and protective shell in this invention; Figure 5 This is an assembly diagram of the mounting base, groove, and inverted T-shaped groove in this invention; Figure 6 This is a schematic diagram of the structure of the pull rod, pin, pull ring, and spring in this invention.
[0018] Explanation of reference numerals in the attached figures: 1-Backplate, 2-Sample pad, 3-Binding pad, 4-Reaction membrane, 5-Absorbent pad, 6-Shell, 7-Observation plate, 8-Sample inlet tube, 9-Sealing film, 10-Cylinder, 11-Observation window, 12-Sample filling through hole, 13-Bearing cap, 14-Snap pin, 15-Mounting base, 16-Groove, 17-Rotating rod, 18-Inverted T-shaped groove, 19-Spring, 20-Fixing shell, 21-Round hole, 22-Slot, 23-Locking element, 24-Pull rod, 25-Pin, 26-Pull ring, 27-Spring. Detailed Implementation
[0019] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0020] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing this invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as limiting this invention.
[0021] In the description of this invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the stated features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified. Furthermore, the terms "installed," "connected," and "linked" should be interpreted broadly, for example, referring to a fixed connection, a detachable connection, or an integral connection; a mechanical connection or an electrical connection; a direct connection or an indirect connection through an intermediate medium; or a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.
[0022] In this embodiment, as Figure 1 and Figure 2As shown, a microbial detection device for antimicrobial peptides includes: a cylinder 10, with an observation window 11 and a sample application through-hole 12 on the cylinder wall; a rotatable mounting base 15 located inside the cylinder 10, the mounting base 15 being configured with mounting positions for mounting multiple test pieces; a rotation positioning mechanism for driving the mounting base 15 to rotate and switch and position it between multiple mounting positions; a support cap 13 screwed onto one end of the cylinder 10; and a fixed shell 20 screwed onto the other end of the cylinder 10. The rotary positioning mechanism is configured to drive the mounting base 15 to rotate, so that any test piece mounted on it enters the testing station and the sample inlet on the test piece is coaxially aligned with the sample feeding through hole 12 on the cylinder 10.
[0023] Specifically, the observation window 11 on the cylinder 10 is made of glass and is used to observe the reaction results of the test strip; the sample dispensing through hole 12 is used to insert a quantitative dropper; the bearing cap 13 is threaded to one end of the cylinder 10; and the fixing shell 20 is threaded to the other end of the cylinder 10.
[0024] In some embodiments, such as Figure 3 and Figure 4 As shown, the test strip includes: a back plate 1, on which a sample pad 2, a conjugate pad 3, a reaction membrane 4 carrying antimicrobial peptide specific recognition molecules, and an absorbent pad 5 are sequentially arranged and partially overlapped in the horizontal direction; a protective shell 6, which is fixed to the top surface of the back plate 1 and covers the above-mentioned pads, and a transparent observation plate 7 is provided on the top surface of the protective shell 6; and a sample inlet tube 8, which is located on the protective shell 6 and directly above the sample pad 2, with a puncturable sealing film 9 covering the top of the sample inlet tube 8; the sample inlet tube 8 constitutes the sample inlet.
[0025] Specifically, the backplate 1 is a supporting substrate; the sample pad 2 is used to receive the sample liquid; the binding pad 3 is pre-embedded with a marker such as a gold-labeled antibody; the reaction membrane 4 is immobilized with antimicrobial peptide specific recognition molecules such as monoclonal antibodies; and the absorbent pad 5 provides capillary force to drive the liquid to flow forward. The protective shell 6 is used to protect the internal structure of the test strip and prevent contamination; the transparent observation plate 7 is designed to facilitate the observation of the signal on the reaction membrane 4. The sample inlet tube 8 is designed on the protective shell 6 to guide the sample to be accurately dropped into the sample pad. The sample inlet tube 8 is sealed with a sealing film 9 to keep the test strip sealed before storage and use.
[0026] In some embodiments, when the test strip is in the testing station, the dropper inserted through the sample dispensing hole 12 can pierce the sealing film 9 and dispense the sample.
[0027] Specifically, the sealing film 9 is an aluminum-plastic composite film or a polymer single film with a thickness of 0.05-0.2 mm. The sealing film 9 has good sealing and puncture resistance, ensuring that the test strip is in a sterile state before storage and use.
[0028] In some embodiments, such as Figure 1 and Figure 6 As shown, the rotary positioning mechanism includes: a rotating rod 17, which is located at one end of the mounting base 15 facing the fixed housing 20. The fixed housing 20 has a circular hole 21 that mates with the rotating rod 17. The rotating rod 17 is rotatably supported in the circular hole 21, and its outer circumference is evenly distributed with multiple slots 22; and a locking member 23, which is located on the fixed housing 20 and includes a pin 25 and an elastic component. The pin 25 engages with the slots 22 under the action of the elastic component to lock the rotational position of the mounting base 15. Specifically, the design of four slots 22 evenly distributed on the outer periphery of the rotating rod 17 facilitates the locking component 23 to fix the rotating rod 17 after it rotates 90 degrees.
[0029] In some embodiments, such as Figure 1 and Figure 5 As shown, a locking pin 14 is hinged to the center of the bearing cover 13, and a groove 16 that matches the locking pin 14 is provided at one end of the mounting base 15. The engagement of the locking pin 14 and the groove 16 realizes the axial positioning of the mounting base 15.
[0030] In some embodiments, such as Figure 6 As shown, the locking component 23 also includes a pull rod 24 and a pull ring 26; the bottom end of the pull rod 24 is integrally formed with a pin 25, and the top end of the pull rod 24 passes through the fixed shell 20 and is fixed with a pull ring 26; by pulling the pull ring 26, the elastic force of the elastic component can be overcome to disengage the pin 25 from the slot 22. Specifically, by pulling the ring 26, the pin 25 can be disengaged from the slot 22, allowing the mounting base 15 to rotate 90° in increments, thereby sequentially aligning the four test pieces with the observation window 11 for testing.
[0031] In some embodiments, such as Figure 6 As shown, the elastic component is a spring 27 sleeved on the pull rod 24.
[0032] Specifically, the spring 27 is sleeved on the pull rod 24 and located between the fixed shell 20 and the pin 25, so as to elastically press the pin 25 into the slot 22, thereby locking the rotation position of the mounting base 15 and aligning the test piece with the observation window 11.
[0033] In some embodiments, such as Figure 1 and Figure 5As shown, the mounting base 15 is rectangular, and inverted T-shaped grooves 18 are provided on all four sides. The back plate 1 of the test piece can be inserted into the inverted T-shaped grooves 18, and spring pieces 19 for locking with the inverted T-shaped grooves 18 are symmetrically provided on both sides of the back plate 1. Specifically, there are four inverted T-shaped grooves 18 for inserting and fixing the test piece; there are two springs 19, both of which are flexible and easy to process.
[0034] In some embodiments, the present invention also provides a method for the microbial detection of antimicrobial peptides, comprising the following steps: Operate the rotary positioning mechanism to rotate the mounting base 15 so that a test piece enters the testing station and is locked in place; A sample containing antimicrobial peptides is added to the test piece at the testing station through the sample addition port 12. The presence of antimicrobial peptides can be determined by reading the signal through observation window 11.
[0035] Specifically, the volume of sample added is 50-200 μL, and the signal reading time is 5-15 min after sample addition.
[0036] The working principle of this application is illustrated below with a preferred embodiment: Insert the quantitative dropper through the sample application port 12 on the cylinder 10. The bottom of the dropper will pierce the sealing film 9 at the top of the sample tube 8 and continue to extend downward until the dropper opening is very close to or slightly in contact with the sample pad 2. Inject 50-200µL of sample solution into the designated area of the sample pad 2. The liquid starts the chromatography process by capillary action, passing through the binding pad 3 in sequence, binding with the marker therein, and then flowing to the reaction membrane 4 pre-embedded with antimicrobial peptide specific antibodies or binding proteins, where a specific antigen-antibody reaction occurs, and finally a detection signal is formed. Insert the back plate 1 into the inverted T-shaped groove 18 of the mounting base 15. The spring 19 is engaged in the inverted T-shaped groove 18. The mounting base 15 can simultaneously hold four test pieces. Then, install the mounting base 15 in the cylinder 10. The groove 16 of the cylinder and the locking pin 14 are engaged together. Use the fixing shell 20 to seal the other end of the mounting base 15. Unlock the rotating rod 17 by pulling the pull ring 26. The operator can rotate it one position every 90° to precisely rotate the next test piece to be tested to the "testing station" formed by the observation window 11 and the sample feeding through hole 12. After releasing the pull ring, the spring 27 will push the pin 25 into the slot 22, firmly locking the mounting base 15 so that the test piece is aligned with the glass observation window 11. Within 5-15 minutes after sample addition, the color band or fluorescence signal appearing on the reaction membrane 4 can be read through the glass observation window 11 on the cylinder 10 and the transparent observation plate 7 of the test strip itself, thereby determining the presence of antimicrobial peptides in each sample.
[0037] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.
Claims
1. A microorganism detection device of an antibacterial peptide, characterized by, The application relates to a test strip rotating and positioning device. The device comprises: a cylinder (10) provided with an observation window (11) and a sample adding through hole (12) on the cylinder wall; a rotatable mounting base (15) arranged in the cylinder (10), the mounting base (15) being provided with mounting stations for mounting a plurality of test strips; a rotating and positioning mechanism for driving the mounting base (15) to rotate and switch and position among the mounting stations; a bearing cover (13) screwed on one end of the cylinder (10); a fixed shell (20) screwed on the other end of the cylinder (10); 2. The antimicrobial peptide-based microorganism detection device according to claim 1, wherein wherein the rotating and positioning mechanism is configured to drive the mounting base (15) to rotate, so that any test strip mounted thereon enters a detection station and the sample inlet on the test strip is coaxially aligned with the sample adding through hole (12) on the cylinder (10). The test strip comprises: a back plate (1) provided with a sample pad (2), a bonding pad (3), a reaction film (4) carrying specific recognition molecules of antibacterial peptide and a water absorption pad (5) in sequence and fixed in partial overlap in the horizontal direction; a protective shell (6) fixed to the top surface of the back plate (1) and wrapping the above-mentioned pad layers, the top surface of the protective shell (6) being provided with a transparent observation plate (7); a sample adding tube (8) arranged on the protective shell (6) and located directly above the sample pad (2), the top end of the sample adding tube (8) being covered with a sealable sealing film (9); 3. The antimicrobial peptide-based microbe detection device of claim 2, wherein the sample adding tube (8) constitutes the sample inlet.
4. The antimicrobial peptide-based microorganism detection device of claim 1, wherein When the test strip is in the detection station, a dropper inserted through the sample adding through hole (12) can pierce the sealing film (9) and add sample. The rotating and positioning mechanism comprises: a rotating rod (17) arranged at one end of the mounting base (15) facing the fixed shell (20), the fixed shell (20) being provided with a round hole (21) matched with the rotating rod (17), the rotating rod (17) being rotatably supported in the round hole (21) and being uniformly provided with a plurality of clamping grooves (22) on the outer periphery; a locking member (23) arranged on the fixed shell (20) and comprising a latch (25) and an elastic component; 5. The antimicrobial peptide-based microbe detection device of claim 1, wherein, the latch (25) is clamped with the clamping grooves (22) under the action of the elastic component, so as to lock the rotating position of the mounting base (15).
6. The antimicrobial peptide-based microbe detection device of claim 4, wherein, The bearing cover (13) is hinged with a latch pin shaft (14) at the center, one end of the mounting base (15) corresponding to the latch pin shaft (14) is provided with a groove (16) matched with the latch pin shaft (14), and the embedding of the latch pin shaft (14) and the groove (16) realizes the axial positioning of the mounting base (15). The locking member (23) further comprises a pull rod (24) and a pull ring (26); the bottom end of the pull rod (24) is integrally formed with the latch (25), and the top end of the pull rod (24) penetrates through the fixed shell (20) and is fixed with the pull ring (26). The elastic force of the elastic component is overcome by pulling the pull ring (26) to disengage the latch (25) from the clamping groove (22).
7. The antimicrobial peptide-based microbe detection device of claim 6, wherein, The elastic component is a spring (27) sleeved on the pull rod (24).
8. The antimicrobial peptide-based microbe detection device of claim 1, wherein, The mounting seat (15) is rectangular, and a reverse T-shaped groove (18) is formed on each side of the mounting seat (15). The back plate (1) of the test strip can be inserted into the reverse T-shaped groove (18), and spring sheets (19) are symmetrically arranged on both sides of the back plate (1) to be clamped with the reverse T-shaped groove (18).
9. A method for detecting microorganisms by using an antibacterial peptide, characterized by, The use of the microbial detection device of any one of claims 1 to 8 comprises the following steps: Operating the rotary positioning mechanism to rotate the mounting seat (15) to make one test strip enter the detection station and be locked; Adding an antibacterial peptide-containing sample to the test strip in the detection station through the sample adding through hole (12); Reading the signal through the observation window (11) to determine the presence of the antibacterial peptide.
10. The method according to claim 9, wherein the method is characterized by, The volume of the added sample is 50-200 μL, and the signal reading time is 5-15 min after the sample is added.