Method for improving selfing affinity of brassica campestris

Abstract

The invention belongs to the technical field of rape breeding, relates to a method for improving selfing affinity of Brassica campestris, and aims to solve the problems that the selfing incompatibility of Brassica campestris is high and the treatment effect of conventional normal saline is unstable. According to the method, a sodium chloride-sodium salicylate solution (the mass concentration of sodium chloride is 1-5% and the mass concentration of sodium salicylate is 1-5% on the basis of water) is sprayed before flowering of rape flower buds, bagging selfing is carried out, and spraying is carried out once every two days. The method preferably sprays to the main inflorescence of the rape, and is suitable for Brassica campestris varieties such as Hengyou 8, Hengyou 6 and the like. The method is easy and convenient to operate, low in cost, small in stigma damage and capable of remarkably improving the selfing angular rate and the affinity index, the effect is improved by 40-50% compared with that of single sodium chloride treatment, the maturing rate is greatly increased, and the method is suitable for large-area breeding application.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of oilseed rape breeding, and relates to a method for improving self-compatibility of Brassica campestris BACKGROUND

[0002] Based on the physiological characteristics of Brassica campestris, the self-incompatibility is strong (self-incompatibility refers to the phenomenon that pollen cannot normally germinate or pollen tube cannot elongate when self-pollination is performed on plants, so that normal fertilization and seed setting cannot be achieved), and the outcrossing seed setting rate reaches 80-90%. The characteristics can prevent close breeding and increase genetic diversity. The self-incompatibility of Brassica campestris makes it more inclined to cross pollination in a natural state, so that a high outcrossing seed setting rate is ensured, and therefore it is difficult to obtain pure line breeding materials in the breeding process. The existing methods generally break the self-incompatibility by adopting methods such as bud stage pollination, CO2 treatment, physiological saline or hormone spraying of the stigma, creating compatible mutants, and using maintainer lines. Since the bud stage pollination method has a large workload, low efficiency and low seed setting rate; the CO2 treatment method needs special equipment and the concentration needs to be optimized; the compatible mutant creation technology has a high threshold and a long cycle; and the use of maintainer line materials is a complex process, the most commonly used method is to spray the stigma with physiological saline before pollination, which has a relatively low technical threshold, does not need special equipment and is easy to operate.

[0003] Generally, 3-5% physiological saline is used to spray and treat the stigma, so as to change the osmotic pressure of the stigma surface or destroy the structure of the recognition protein, thereby temporarily inhibiting the self-incompatibility. However, the method is limited by the spraying period, environmental control and other factors in the actual operation process, and the effect often cannot reach the ideal state, the self-seeding rate of oilseed rape is still low, and the effect is more difficult to achieve the expectation when a large area is treated. SUMMARY

[0004] In order to solve the above problems, the application provides a method for improving the self-compatibility of Brassica campestris, wherein 15 kg / acre of a sodium chloride-sodium salicylate solution is sprayed before the opening of the oilseed rape buds, and the self-pollination is bagged, and the spraying is performed once every two days.

[0005] The sodium chloride-sodium salicylate solution has a sodium chloride mass concentration of 1-5% and a sodium salicylate mass concentration of 1-5% based on water.

[0006] Preferably, the sodium chloride mass concentration is 1%, and the sodium salicylate mass concentration is 1%.

[0007] Preferably, the sodium chloride mass concentration is 3%, and the sodium salicylate mass concentration is 3%.

[0008] Preferably, the sodium chloride mass concentration is 5%, and the sodium salicylate mass concentration is 5%.

[0009] Preferably, the oilseed rape varieties are Hengyou No. 8 and Hengyou No. 6.

[0010] Preferably, the spraying is performed on the main inflorescences of the oilseed rape.

[0011] The present application has the following advantages:

[0012] (1) Easy to operate and low in cost.

[0013] (2) High in efficiency, high in seed setting and good in effect. The effect is much higher than that of the single treatment material, a large amount of experimental materials can be treated at one time, and a sprayer can be directly used for spraying in large-area application.

[0014] (3) Less damage to the stigma of the oilseed rape, and the pollination effect is greatly improved.

[0015] (4) The self-compatible effect is increased by 40-50%, and the seed setting rate is greatly improved. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present application will be described below in a clear and complete manner. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0017] Embodiment 1

[0018] Test varieties: Hengyou No. 8 and Hengyou No. 6. Since the side branch development of the Chinese cabbage type oilseed rape is quite different, the main inflorescences of the oilseed rape are used for the test in the present test.

[0019] Experimental steps: a total of 7 treatments, control (CK water), 1% NaCl solution, 3% NaCl solution, 5% NaCl solution, 1% NaCl solution + 1% sodium salicylate solution, 3% NaCl solution + 3% sodium salicylate solution, and 5% NaCl solution + 5% sodium salicylate solution (if the concentration of the NaCl solution exceeds 5%, the flowers will wilt and the petals will fall off, so the upper limit of the concentration of the NaCl solution in the test is set to 5%). Ten plants with consistent growth vigor are selected from each of the two Chinese cabbage type winter oilseed rape varieties Hengyou No. 8 and Hengyou No. 6, the middle buds in the main inflorescences are removed with tweezers, about 20 strong buds are reserved in each inflorescence, the hand-held compressed sprayer is used for spraying treatment before flowering, and then bagged selfing is performed, and the spraying is performed once every two days.

[0020] Detection index: the number of silique and the number of seeds per silique under different treatments are investigated when the fruiting branches are yellow and mature, the silique setting rate and the compatibility index (seed number / pollinated flower silique number) of different treatments are calculated, and the results are shown in Tables 1-2.

[0021] Table 1 Silique setting rate of Chinese cabbage type winter oilseed rape under different treatments

[0022]

[0023] Table 2 Affinity index of different treatments of Brassica campestris L.

[0024]

[0025] As can be seen from Table 1, after spraying different concentrations of NaCl solution, the number of nodes and the node rate of Brassica campestris L. were significantly improved compared with the water control, and after adding the same proportion of sodium salicylate solution in the NaCl solution, the number of nodes and the node rate were significantly improved compared with the effect of pure NaCl solution. Among them, the node rate of 3% NaCl + 3% sodium salicylate treatment and 5% NaCl + 5% sodium salicylate treatment was significantly different from that of 1% NaCl + 1% sodium salicylate treatment, and there was no significant difference between 3% NaCl + 3% sodium salicylate treatment and 5% NaCl + 5% sodium salicylate treatment.

[0026] As can be seen from Table 2, after NaCl + sodium salicylate treatment, the affinity index of Brassica campestris L. was significantly improved compared with pure NaCl solution treatment. In two test varieties, the affinity index of Brassica campestris L. was the highest under 3% NaCl + 3% sodium salicylate treatment, and the affinity index reached more than 5.80, and the affinity index was improved by more than 40% compared with pure 3% NaCl solution treatment; while under 5% NaCl + 5% sodium salicylate treatment, the affinity index of Brassica campestris L. was slightly lower than that under 3% NaCl + 3% sodium salicylate treatment, but the difference was not significant.

[0027] Based on the above test results, through the analysis of the node rate and affinity index of Brassica campestris L. treated by spraying different concentrations of NaCl solution and NaCl + sodium salicylate, the use of 3-5% physiological saline and the same proportion of sodium salicylate to spray the flower buds before the flowering of Brassica campestris L. can improve the selfing effect by 40-50% compared with the use of physiological saline alone.

Claims

1. A method for increasing self-compatibility in Brassica campestris, characterized by, Spraying 15 kg / mu of sodium chloride-sodium salicylate solution before the rape flower bud opens, bagging selfing, spraying once every two days; The mass concentration of sodium chloride is 1-5% and the mass concentration of sodium salicylate is 1-5% based on water.

2. The method for improving self-compatibility of Brassica campestris according to claim 1, wherein, The mass concentration of sodium chloride is 1% and the mass concentration of sodium salicylate is 1%.

3. The method for improving self-compatibility of Brassica campestris according to claim 1, wherein, The mass concentration of sodium chloride is 3% and the mass concentration of sodium salicylate is 3%.

4. The method for improving self-compatibility of Brassica campestris according to claim 1, wherein, The mass concentration of sodium chloride is 5% and the mass concentration of sodium salicylate is 5%.

5. The method for improving self-compatibility of Brassica campestris according to claim 1, wherein, The rape varieties are Hengyou No. 8 and Hengyou No.

6.

6. The method for improving self-compatibility of Brassica campestris according to claim 1, wherein, Spraying on the main inflorescence of rape.

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