Method for artificially propagating Mimeria champaca

Abstract

The artificial propagation method comprises the following steps: step 1, constructing an artificial propagation device, wherein the artificial propagation device comprises a plurality of layers of propagation units, an environment regulation and control module and a monitoring module; step 2, preparation of an artificial breeding substrate: determining components of the artificial breeding substrate, pretreating the raw materials, then adding a microbial inoculum and nutrients, and finally adjusting the humidity; and step 3, regulating and controlling artificial breeding conditions: adopting the device and the substrate to carry out artificial propagation of the michelia champaca, and carrying out staged regulation and control. By customizing an artificial breeding device, optimizing a breeding matrix formula and accurately regulating and controlling breeding conditions, the large-scale propagation targets that the Burmese egg hatching rate is larger than or equal to 90%, the larva survival rate is larger than or equal to 95% and the adult mating rate is larger than or equal to 85% are achieved.

Description

Technical Field

[0001] This invention relates to the field of artificial insect breeding technology, and in particular to a method for artificial propagation of Burmese worms. Background Technology

[0002] As a key organism for soil ecological restoration research, the wild population of Burmese worm is difficult to obtain, its numbers are unstable, and it is significantly affected by the natural environment (such as temperature, humidity, and natural enemies), making it difficult to meet the large-scale needs of scientific research experiments or ecological applications.

[0003] Existing insect propagation technologies mostly target common species such as butterflies and bees, and lack specific solutions for Burmese worms. On the one hand, general breeding devices cannot accurately control soil moisture (the eggs of the Burmese worm require high humidity to hatch but cannot tolerate water accumulation), which can easily lead to moldy eggs or dehydration and death of larvae; On the other hand, conventional substrates (such as ordinary garden soil and leaf mold) are nutritionally unbalanced and cannot meet the feeding needs of Burmese worm larvae for microorganisms (fungi and algae), resulting in a larval survival rate of less than 30%. Furthermore, existing technologies do not clearly define the differentiated condition parameters for each developmental stage (egg, larva, and adult) of the Burmese worm, which can easily lead to problems such as low adult mating rates and disordered egg hatching cycles.

[0004] Therefore, in order to solve the problems of "low propagation efficiency, unstable population and poor adaptability" in the existing technology, it is an urgent technical problem to be solved by those skilled in the art to provide a three-in-one "device-substrate-condition" artificial propagation method for Burmese worms. Summary of the Invention

[0005] In view of this, the present invention provides a method for the artificial propagation of Burmese worms. By customizing artificial breeding equipment, optimizing the breeding substrate formula and precisely controlling the breeding conditions, the method achieves the goal of large-scale propagation of Burmese worms with an egg hatching rate of ≥90%, a larval survival rate of ≥95%, and an adult mating rate of ≥85%.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: A method for artificial propagation of *Burmaea spp.* includes the following steps: Step 1: Construct artificial breeding equipment The artificial breeding device includes a multi-layered breeding unit, an environmental control module, and a monitoring module. Step 2: Preparation of artificial breeding substrate The composition of the artificial breeding substrate is determined, the raw materials are pretreated first, then microbial agents and nutrients are added, and finally the humidity is adjusted. Step 3: Control of artificial breeding conditions The above-mentioned device and substrate were used to artificially propagate Burmese worms, and the process was carried out in stages.

[0007] Preferably, in step 1, the multi-layer breeding unit, environmental control module, and monitoring module have the following specific structure: Multi-layer breeding unit: Made of transparent acrylic material, the overall structure is a cuboid with a length of 80cm × width of 50cm × height of 10cm per layer, with a total of 3-5 layers and a layer spacing of 30cm; each layer of the breeding unit has 5-8 water-permeable holes with a diameter of 2cm at the bottom, and the holes are covered with 100-mesh nylon mesh. A water-receiving tray with a height of 3cm is placed below the water-permeable holes; the top of the inner wall of the breeding unit has a ring of anti-escape protrusions, 5cm wide, inclined inward at 45°, and made of polytetrafluoroethylene. Environmental control module: including humidity control components, temperature control components and ventilation components; The humidity control component is a layered atomizing nozzle, with two nozzles at the top of each breeding unit. It is connected to a programmable timer, which can set the spray frequency. It is equipped with a 10L water tank and a micro water pump with a head of ≥2m. The temperature regulation component is a heating film attached to the outside of the breeding unit, with a power of 50W / layer. It is connected to a temperature controller, with a temperature control accuracy of ±0.5℃, and is equipped with a cooling fan that automatically starts when the temperature exceeds the set value. The ventilation components are louvers on both sides of the device, with manually adjustable opening and closing, and an exhaust fan at the top with a power of 15W, set to run for 15 minutes every 2 hours. Monitoring module: Includes layered temperature and humidity sensors, one of which is placed in each breeding unit, with an accuracy of ±0.2℃ for temperature and ±2% RH for humidity, and a camera, one of which is located on the top of the device, to observe the activity status of Burmese worms in real time. Sensor data and camera images can be wirelessly transmitted to the terminal.

[0008] Preferably, in step 2, the artificial propagation substrate consists of the following components: 40-50 parts leaf mold, 20-25 parts decomposed mulberry branch powder, 15-20 parts peat moss, 5-8 parts perlite, 2-3 parts compound microbial agent, 1-2 parts sucrose, and 0.5-1 parts calcium carbonate.

[0009] Preferably, in step 2, the preparation method includes the following steps: Step 2.1: Raw material pretreatment Mix leaf mold, decomposed mulberry branch powder, peat moss and perlite, and stir well to obtain the basic substrate. Step 2.2: Microbial Agent and Nutrient Addition Add the compound microbial agent, sucrose and calcium carbonate to the base matrix while stirring at 150 r / min for 10-15 minutes. Step 2.3: Humidity Adjustment Add deionized water to the mixed substrate and adjust the substrate humidity to 65%-70%. Then, put the substrate into the breeding unit, adjust the substrate pH to 6.8-7.2, and lay it to a thickness of 5-6 cm. Let it stand for 24 hours before use.

[0010] Preferably, in step 2.1, the leaf mold is collected from broad-leaved forests and crushed to a particle size ≤5mm; the decomposed mulberry branch powder is made by crushing mulberry branches to a particle size ≤3mm after high-temperature decomposition for 3 months; the pH of the peat soil is adjusted to 6.5-7.0; and the perlite is sieved through a 5mm sieve to remove impurities. In step 2.2, the compound microbial agent contains Bacillus subtilis, Trichoderma harzianum, Streptomyces cerevisiae, and Nocardia asteroides in a concentration ratio of 2:3:3:2, with an effective viable count ≥5×10⁻⁶. 8 CFU / g.

[0011] Preferably, in step 3, the control is performed in stages according to the following conditions: Phase 1: Adult release and mating stage: (1) Release density: Select healthy adults with a body length of 1.0-1.2cm and a female-to-male ratio of 1:1, and release them at a density of 9-12 individuals / m². 2 The density was applied to the substrate surface of the breeding unit; (2) Environmental parameters: Temperature is controlled at 22-25℃, humidity is controlled at 75%-80%, and the light intensity is 12h light / 12h darkness; (3) Feeding management: Feed the insects with special feed once a day, with the amount being 5%-8% of the total weight of the adults. Clean up any leftover feed 24 hours after feeding. (4) Mating rate observation: Observe the insect body through the camera. If the female insect body is lying still and milky white spots can be seen on the ventral cyst body, the mating is successful. Calculate the number of insect bodies that have successfully mated and calculate the mating rate.

[0012] Second stage: Egg hatching stage (1) Substrate adjustment: Transfer the female insects that have successfully mated to a new breeding unit, and cover the surface of the original breeding unit substrate with a 2cm thick layer of sterile decaying leaves; (2) Environmental parameters: Temperature increased to 25-28℃, humidity maintained at 75%-80%, and lighting adjusted to complete darkness; (3) Hatching monitoring: Check the temperature and humidity data through the terminal every day, and sample the substrate every 3 days. If the eggs crack, white larvae crawl out of the egg shell and the larvae can move normally, it is judged as successful hatching; if the eggs are shriveled, moldy, or the embryos turn black and die, it is judged as unhatched; if no new larvae hatch for 3 consecutive days, the hatching process is judged to be over, and the number of successfully hatched larvae, egg hatching cycle and weight of 100 eggs are recorded. Third stage: Larval stage (1) Density control: After hatching within 24 hours, the larvae should be promptly transferred to other breeding units, with a breeding density of 200-250 larvae / m². 2 ; (2) Environmental parameters: Temperature controlled at 23-26℃, humidity reduced to 70%-75%, and light intensity restored to 12h light / 12h darkness; (3) Feeding Management: When the larvae are less than 0.3 cm in length, i.e., 1-15 days after hatching, feed them diluted microbial solution once a day; when the larvae are more than 0.4 cm in length, i.e., after 15 days of hatching, add decomposed mulberry twigs with a particle size ≤1 mm to the microbial solution once a day, at a rate of 1 mm per m. 2 Feed 100g; (4) Substrate replacement: Replace the surface substrate every 10 days with a thickness of 2cm and replenish with newly prepared breeding substrate; (5) Observation of larval survival rate: From the day the larvae hatch, observe once a day at 9 o'clock until the larvae's abdominal limbs appear bristle-like or swollen. Record the number of larvae that crawl flexibly, whose appendages can extend normally, and that are healthy. Calculate the larval survival rate, larval stage, and weight of 100 larvae. Fourth stage: Adult stage (1) Adult maturation: When bristles or swelling grow on the abdomen of the insect, that is, when the body length is 1.0-1.2cm and the body color is gray or blackish-gray, transfer it to the maturation and breeding unit. The breeding unit is covered with a 3cm thick substrate of sterile perlite and leaf mold in a 1:1 ratio, with a humidity of 65%, a temperature of 24-25℃, a humidity of 70%, and a light exposure of 10h light / 14h darkness. (2) Collection of breeding insects: Collect adult male and female insects with blackish-gray body color using an insect suction device. The female insects should be no less than 1.2 cm in length and the male insects should be no less than 1.0 cm in length. Keep them in a male-to-female ratio of 2:1. The rest are used for experiments or ecological applications.

[0013] Preferably, in the first stage (2), the atomizing nozzle sprays once every 4 hours for 10 seconds each time, using a 40W LED cold light lamp to avoid direct exposure to strong light; In the first stage (2), the special feed is prepared by weight as follows: 15 parts cuttlebone powder, 30 parts wheat bran, 25 parts fermented soybean meal, 5 parts yeast powder, and 0.5 parts vitamin C, mixed and crushed to a particle size ≤1mm; In the second stage (1), sterile decaying leaves are sterilized at 121°C for 30 minutes under high pressure while maintaining a humidity of 70%; In the second stage (2), the atomizing nozzle sprays once every 6 hours, for 8 seconds each time; In the third stage (2), the atomizing nozzle sprays once every 8 hours for 5 seconds each time, and the LED light brightness is adjusted to 100 lux to simulate a natural low light environment; In the third stage (3), the microbial inoculum is a compound microbial agent diluted with water at a ratio of 1:50, per m 2 Spray 500mL; In the fourth stage (1), a 3cm thick substrate of sterile perlite, leaf mold and decomposed mulberry branches is laid in the breeding unit of the maturation unit, with a ratio of 1:1:1 and a humidity of 65%.

[0014] The present invention achieves the following technical effects compared to the prior art: (1) This invention achieves precise control of the breeding environment (temperature, humidity, ventilation, and light) of Burmese worms by customizing artificial breeding devices, solves the problem of egg mold caused by humidity fluctuations in the natural environment, and increases the egg hatching rate from 30% in the existing technology to more than 90%. (2) The special breeding substrate of this invention provides natural nutrition through leaf mold and decomposed mulberry branch powder, and regulates the substrate micro-ecology with compound microbial agents to meet the feeding needs of Burmese worm larvae for microorganisms, thereby increasing the larval survival rate to over 95% and shortening the larval development cycle by 15%-20%; (3) The present invention regulates the breeding conditions (differentiated parameters of eggs, larvae and adults) in stages to meet the needs of each developmental stage. The mating rate of adults reaches more than 85%, and the single propagation cycle (from egg to adult) is controlled within 60-70 days, realizing large-scale and stable propagation. (4) The device of the present invention adopts a multi-layer structure, which improves the space utilization rate by 2-3 times, and is equipped with a remote monitoring module to reduce the cost of manual intervention, making it suitable for laboratory or factory mass propagation. Attached Figure Description

[0015] Figure 1 This is a flowchart of the present invention. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Example 1: Step 1: Construct artificial breeding equipment The artificial breeding device includes a multi-layered breeding unit, an environmental control module, and a monitoring module. Step 2: Preparation of artificial breeding substrate The composition of the artificial breeding substrate is determined, the raw materials are pretreated first, then microbial agents and nutrients are added, and finally the humidity is adjusted. Step 3: Control of artificial breeding conditions The above-mentioned device and substrate were used to artificially propagate Burmese worms, and the process was carried out in stages.

[0019] In step 1, the multi-layered breeding unit, environmental control module, and monitoring module have the following specific structure: Multi-layered breeding unit: Made of transparent acrylic material, the overall structure is a cuboid with a length of 80cm × width of 50cm × height of 10cm per layer, with a total of 3-5 layers and a layer spacing of 30cm to facilitate ventilation; each breeding unit has 5-8 water-permeable holes with a diameter of 2cm at the bottom, and the holes are covered with 100-mesh nylon mesh to prevent larvae from escaping and avoid substrate loss. A water-receiving tray with a height of 3cm is placed below the water-permeable holes to collect excess water; the top of the inner wall of the breeding unit has a ring of escape-prevention protrusions, 5cm wide, inclined inward at 45°, made of polytetrafluoroethylene, which uses the smooth surface to prevent adult insects from climbing and escaping; Environmental control module: including humidity control components, temperature control components and ventilation components; The humidity control component consists of layered atomizing nozzles, with two nozzles on the top of each breeding unit. These nozzles are connected to a programmable timer, allowing the spray frequency to be set. The unit is equipped with a 10L water tank and a micro water pump with a head of ≥2m. The temperature control component is a heating film attached to the outside of the breeding unit, with a power of 50W / layer. It is connected to a temperature controller, with a temperature control accuracy of ±0.5℃. It is equipped with a cooling fan that automatically starts when the temperature exceeds the set value. The ventilation components consist of louvers on both sides of the device, with manually adjustable opening and closing, and an exhaust fan at the top with a power of 15W, set to run for 15 minutes every 2 hours to maintain air circulation. Monitoring module: Includes layered temperature and humidity sensors, one of which is placed in each breeding unit, with an accuracy of ±0.2℃ for temperature and ±2% RH for humidity, and a camera, one of which is located on the top of the device, to observe the activity status of Burmese worms in real time. Sensor data and camera images can be wirelessly transmitted to the terminal to achieve remote monitoring.

[0020] In step 2, the artificial propagation substrate consists of the following components: 40 parts leaf mold, 20 parts decomposed mulberry branch powder, 15 parts peat moss, 5 parts perlite, 2 parts compound microbial inoculant, 1 part sucrose, and 0.5 parts calcium carbonate.

[0021] Step 2, the preparation method includes the following steps: Step 2.1: Raw material pretreatment Mix leaf mold, decomposed mulberry branch powder, peat moss and perlite, and stir well to obtain the basic substrate. Step 2.2: Microbial Agent and Nutrient Addition Add the compound microbial agent, sucrose and calcium carbonate to the base matrix while stirring at 150 r / min for 10 minutes. Step 2.3: Humidity Adjustment Add deionized water to the mixed substrate, adjust the substrate humidity to 65%, then put the substrate into the breeding unit, adjust the substrate pH to 6.8, lay it to a thickness of 5cm, and let it stand for 24 hours before use.

[0022] In step 2.1, the leaf mold was collected from broad-leaved forests and crushed to a particle size of ≤5mm. The decomposed mulberry branch powder was made by crushing mulberry branches to a particle size of ≤3mm after high-temperature decomposition for 3 months. The pH of the peat soil was adjusted to 6.5. The perlite was sieved through a 5mm sieve to remove impurities. In step 2.2, the compound microbial agent contains Bacillus subtilis, Trichoderma harzianum, Streptomyces cerevisiae, and Nocardia asteroides in a concentration ratio of 2:3:3:2, with an effective viable count ≥5×10⁻⁶. 8 CFU / g.

[0023] In step 3, adjustments will be made in stages according to the following conditions: Phase 1: Adult release and mating stage: (1) Release density: Select healthy adults with a body length of 1.0 cm and a female-to-male ratio of 1:1, and release them at a density of 10 individuals / m². 2 The density was applied to the substrate surface of the breeding unit; (2) Environmental parameters: temperature controlled at 22℃, humidity controlled at 75%, and light intensity at 12h light / 12h darkness; (3) Feeding management: Feed the insects with special feed once a day, and the amount of feed is 5% of the total weight of the adult insects. Clean up the remaining feed 24 hours after feeding to prevent mold. (4) Mating observation: Observe the behavior of the female insect through the camera. If the female insect is lying still and milky white spots can be seen on the ventral cyst, the mating is successful. Calculate the number of insects that have successfully mated and calculate the mating rate.

[0024] Second stage: Egg hatching stage (1) Substrate adjustment: Transfer the female insects that have successfully mated to a new breeding unit, leave 200 eggs on the surface of the substrate in the breeding unit, and cover them with a 2cm thick layer of sterile decaying leaves; (2) Environmental parameters: Temperature increased to 25℃, humidity maintained at 75%, and lighting adjusted to complete darkness; (3) Hatching monitoring: Check the temperature and humidity data through the terminal every day. If the eggs crack, white larvae crawl out of the eggshell, and the larvae can move normally (crawling, extending appendages), it is judged as successful hatching; if the eggs are shriveled, moldy, or the embryos turn black and die, it is judged as unhatched; if no new larvae hatch for 3 consecutive days, the hatching process is judged to be over. Record the number of successfully hatched larvae, the egg hatching cycle, and the weight of 100 eggs. Third stage: Larval rearing stage (1) Density control: Larvae hatched within 48 hours should be promptly transferred to other breeding units and bred at a density of 200 larvae / m². 2 To avoid excessive larval density leading to competition for nutrients and affecting insect development; (2) Environmental parameters: Temperature controlled at 23℃, humidity reduced to 70%, and light intensity restored to 12h light / 12h darkness; (3) Feeding Management: When the larvae are less than 0.3 cm in length, i.e., 1-15 days after hatching, feed them diluted microbial solution once a day; when the larvae are more than 0.4 cm in length, i.e., after 15 days of hatching, add decomposed mulberry branch chips (≤1 mm in diameter) to the microbial solution once a day, per m 2 Feed 100g to avoid competition for nutrients among the larvae; (4) Substrate replacement: Replace the surface substrate every 10 days with a thickness of 2cm and replenish with newly prepared breeding substrate to avoid depletion of substrate nutrients or accumulation of harmful substances; (5) Observation of larval survival rate: From the day the larvae hatch, observe once a day at 9 o'clock until the larvae's abdominal limbs appear bristle-like or swollen. Record the number of healthy larvae that can crawl flexibly and whose appendages can extend normally. Calculate the larval survival rate, larval stage, and weight of 100 larvae.

[0025] Fourth stage: Adult maturation stage (1) Adult maturation: When the abdominal limbs of the insect appear bristle-like or swollen, that is, when the body length is 1.0cm and the body color is gray or blackish-gray, transfer it to the maturation and breeding unit. The breeding unit is covered with a 3cm thick substrate of sterile perlite and leaf mold in a 1:1 ratio, with a humidity of 65%, a temperature of 24℃, a humidity of 70%, and a light exposure of 10h light / 14h darkness. (2) Collection of breeding insects: Collect adult male and female insects with blackish-gray body color using an insect suction device. The female insects should be no less than 1.2 cm in length and the male insects should be no less than 1.0 cm in length. They should be retained at a ratio of 2:1 for females and males. The rest are used for experiments or ecological applications.

[0026] In the first stage (2), the atomizing nozzle sprays once every 4 hours for 10 seconds each time, using a 40W LED cold light lamp to avoid direct strong light; In the first stage (2), the special feed is prepared by weight as follows: 15 parts cuttlebone powder, 30 parts wheat bran, 25 parts fermented soybean meal, 5 parts yeast powder, and 0.5 parts vitamin C, mixed and crushed to a particle size ≤1mm; In the second stage (1), the sterile decaying leaves were sterilized at 121℃ for 30 minutes under high pressure, while maintaining a humidity of 70%; In the second stage (2), the atomizing nozzle sprays once every 6 hours, for 8 seconds each time; In the third stage (2), the atomizing nozzle sprays once every 8 hours for 5 seconds each time, and the LED light brightness is adjusted to 100 lux to simulate a natural low light environment; In the third stage (3), the microbial inoculum is a compound microbial agent diluted with water at a ratio of 1:50, per m 2 Spray 500mL; In the fourth stage (1), a 3cm thick substrate of sterile perlite, leaf mold and decomposed mulberry branches was laid in the breeding unit of the maturation unit, with a ratio of 1:1:1 and a humidity of 65%.

[0027] Example 2: Step 1: Construct artificial breeding equipment The artificial breeding device includes a multi-layered breeding unit, an environmental control module, and a monitoring module. Step 2: Preparation of artificial breeding substrate The composition of the artificial breeding substrate is determined, the raw materials are pretreated first, then microbial agents and nutrients are added, and finally the humidity is adjusted. Step 3: Control of artificial breeding conditions The above-mentioned device and substrate were used to artificially propagate Burmese worms, and the process was carried out in stages.

[0028] In step 1, the multi-layered breeding unit, environmental control module, and monitoring module have the following specific structure: Multi-layered breeding unit: Made of transparent acrylic material, the overall structure is a cuboid with a length of 80cm × width of 50cm × height of 10cm per layer, with a total of 3-5 layers and a layer spacing of 30cm to facilitate ventilation; each breeding unit has 5-8 water-permeable holes with a diameter of 2cm at the bottom, and the holes are covered with 100-mesh nylon mesh to prevent larvae from escaping and avoid substrate loss. A water-receiving tray with a height of 3cm is placed below the water-permeable holes to collect excess water; the top of the inner wall of the breeding unit has a ring of escape-prevention protrusions, 5cm wide, inclined inward at 45°, made of polytetrafluoroethylene, which uses the smooth surface to prevent adult insects from climbing and escaping; Environmental control module: including humidity control components, temperature control components and ventilation components; The humidity control component consists of layered atomizing nozzles, with two nozzles on the top of each breeding unit. These nozzles are connected to a programmable timer, allowing the spray frequency to be set. The unit is equipped with a 10L water tank and a micro water pump with a head of ≥2m. The temperature control component is a heating film attached to the outside of the breeding unit, with a power of 50W / layer. It is connected to a temperature controller, with a temperature control accuracy of ±0.5℃. It is equipped with a cooling fan that automatically starts when the temperature exceeds the set value. The ventilation components consist of louvers on both sides of the device, with manually adjustable opening and closing, and an exhaust fan at the top with a power of 15W, set to run for 15 minutes every 2 hours to maintain air circulation. Monitoring module: Includes layered temperature and humidity sensors, one of which is placed in each breeding unit, with an accuracy of ±0.2℃ for temperature and ±2% RH for humidity, and a camera, one of which is located on the top of the device, to observe the activity status of Burmese worms in real time. Sensor data and camera images can be wirelessly transmitted to the terminal to achieve remote monitoring.

[0029] In step 2, the artificial propagation substrate consists of the following components: 45 parts leaf mold, 22 parts decomposed mulberry branch powder, 18 parts peat moss, 6 parts perlite, 2 parts compound microbial inoculant, 1 part sucrose, and 1 part calcium carbonate.

[0030] Step 2, the preparation method includes the following steps: Step 2.1: Raw material pretreatment Mix leaf mold, decomposed mulberry branch powder, peat moss and perlite, and stir well to obtain the basic substrate. Step 2.2: Microbial Agent and Nutrient Addition Add the compound microbial agent, sucrose and calcium carbonate to the base matrix while stirring at 150 r / min for 12 minutes. Step 2.3: Humidity Adjustment Add deionized water to the mixed substrate and adjust the substrate humidity to 68%. Then, put the substrate into the breeding unit, adjust the substrate pH to 7.0, lay it to a thickness of 5cm, and let it stand for 24 hours before use.

[0031] In step 2.1, the leaf mold was collected from broad-leaved forests and crushed to a particle size of ≤5mm. The decomposed mulberry branch powder was made by crushing mulberry branches to a particle size of ≤3mm after high-temperature decomposition for 3 months. The pH of the peat soil was adjusted to 6.8. The perlite was sieved through a 5mm sieve to remove impurities. In step 2.2, the compound microbial agent contains Bacillus subtilis, Trichoderma harzianum, Streptomyces cerevisiae, and Nocardia asteroides in a concentration ratio of 2:3:3:2, with an effective viable count ≥5×10⁻⁶. 8 CFU / g.

[0032] In step 3, adjustments will be made in stages according to the following conditions: Phase 1: Adult release and mating stage: (1) Release density: Select healthy adults with a body length of 1.1cm and a male-to-female ratio of 1:1, and release them at a density of 10 individuals / m². 2 The density was applied to the substrate surface of the breeding unit; (2) Environmental parameters: temperature controlled at 23℃, humidity controlled at 78%, and light intensity at 12h light / 12h darkness; (3) Feeding management: Feed the insects with special feed once a day, and the amount of feed is 6% of the total weight of the adult insects. Clean up the leftover feed 24 hours after feeding to prevent mold. (4) Mating observation: The behavior of female insects was observed through a camera. A total of 9 female insects were found to be lying still within 3 days, and milky white spots were visible on their ventral cysts. The mating rate was calculated to be 90%.

[0033] Second stage: Egg hatching stage (1) Substrate adjustment: Transfer the female insects that have successfully mated to a new breeding unit, leave 200 eggs on the surface of the substrate in the breeding unit, and cover them with a 2cm thick layer of sterile decaying leaves; (2) Environmental parameters: temperature increased to 26℃, humidity maintained at 78%, and lighting adjusted to complete darkness; (3) Hatching monitoring: Check the temperature and humidity data through the terminal every day. If the eggs crack, white larvae crawl out of the eggshell, and the larvae can move normally (crawling, extending appendages), it is judged as successful hatching; if the eggs are shriveled, moldy, or the embryos turn black and die, it is judged as unhatched; if no new larvae hatch for 3 consecutive days, the hatching process is judged to be over. Record the number of successfully hatched larvae, the egg hatching cycle, and the weight of 100 eggs. Third stage: Larval rearing stage (1) Density control: Larvae hatched within 48 hours should be promptly transferred to other breeding units and bred at a density of 200 larvae / m². 2 To avoid excessive larval density leading to competition for nutrients and affecting insect development; (2) Environmental parameters: Temperature controlled at 24℃, humidity reduced to 72%, and light intensity restored to 12h light / 12h darkness; (3) Feeding Management: When the larvae are less than 0.3 cm in length, i.e., 1-15 days after hatching, feed them diluted microbial solution once a day; when the larvae are more than 0.4 cm in length, i.e., after 15 days of hatching, add decomposed mulberry branch chips (≤1 mm in diameter) to the microbial solution once a day, per m 2 Feed 100g to avoid competition for nutrients among the larvae; (4) Substrate replacement: Replace the surface substrate every 10 days with a thickness of 2cm and replenish with newly prepared breeding substrate to avoid depletion of substrate nutrients or accumulation of harmful substances; (5) Observation of larval survival rate: From the day the larvae hatch, observe once a day at 9 o'clock until the larvae's abdominal limbs appear bristle-like or swollen. Record the number of healthy larvae that can crawl flexibly and whose appendages can extend normally. Calculate the larval survival rate, larval stage, and weight of 100 larvae.

[0034] Fourth stage: Adult maturation stage (1) Adult maturation: When the abdominal limbs of the insect appear bristle-like or swollen, that is, when the body length is 1.1cm and the body color is gray or blackish-gray, transfer it to the maturation and breeding unit. The breeding unit is covered with a 3cm thick substrate of sterile perlite and leaf mold in a 1:1 ratio, with a humidity of 65%, a temperature of 24℃, a humidity of 70%, and a light exposure of 10h light / 14h darkness. (2) Collection of breeding insects: Collect adult male and female insects with blackish-gray body color using an insect suction device. The female insects should be no less than 1.2 cm in length and the male insects should be no less than 1.0 cm in length. They should be retained at a ratio of 2:1 for females and males. The rest are used for experiments or ecological applications.

[0035] In the first stage (2), the atomizing nozzle sprays once every 4 hours for 10 seconds each time, using a 40W LED cold light lamp to avoid direct strong light; In the first stage (2), the special feed is prepared by weight as follows: 15 parts cuttlebone powder, 30 parts wheat bran, 25 parts fermented soybean meal, 5 parts yeast powder, and 0.5 parts vitamin C, mixed and crushed to a particle size ≤1mm; In the second stage (1), the sterile decaying leaves were sterilized at 121℃ for 30 minutes under high pressure, while maintaining a humidity of 70%; In the second stage (2), the atomizing nozzle sprays once every 6 hours, for 8 seconds each time; In the third stage (2), the atomizing nozzle sprays once every 8 hours for 5 seconds each time, and the LED light brightness is adjusted to 100 lux to simulate a natural low light environment; In the third stage (3), the microbial inoculum is a compound microbial agent diluted with water at a ratio of 1:50, per m 2 Spray 500mL; In the fourth stage (1), a 3cm thick substrate of sterile perlite, leaf mold and decomposed mulberry branches was laid in the breeding unit of the maturation unit, with a ratio of 1:1:1 and a humidity of 65%.

[0036] Example 3: Example 1: Step 1: Construct artificial breeding equipment The artificial breeding device includes a multi-layered breeding unit, an environmental control module, and a monitoring module. Step 2: Preparation of artificial breeding substrate The composition of the artificial breeding substrate is determined, the raw materials are pretreated first, then microbial agents and nutrients are added, and finally the humidity is adjusted. Step 3: Control of artificial breeding conditions The above-mentioned device and substrate were used to artificially propagate Burmese worms, and the process was carried out in stages.

[0037] In step 1, the multi-layered breeding unit, environmental control module, and monitoring module have the following specific structure: Multi-layered breeding unit: Made of transparent acrylic material, the overall structure is a cuboid with a length of 80cm × width of 50cm × height of 10cm per layer, with a total of 3-5 layers and a layer spacing of 30cm to facilitate ventilation; each breeding unit has 5-8 water-permeable holes with a diameter of 2cm at the bottom, and the holes are covered with 100-mesh nylon mesh to prevent larvae from escaping and avoid substrate loss. A water-receiving tray with a height of 3cm is placed below the water-permeable holes to collect excess water; the top of the inner wall of the breeding unit has a ring of escape-prevention protrusions, 5cm wide, inclined inward at 45°, made of polytetrafluoroethylene, which uses the smooth surface to prevent adult insects from climbing and escaping; Environmental control module: including humidity control components, temperature control components and ventilation components; The humidity control component consists of layered atomizing nozzles, with two nozzles on the top of each breeding unit. These nozzles are connected to a programmable timer, allowing the spray frequency to be set. The unit is equipped with a 10L water tank and a micro water pump with a head of ≥2m. The temperature control component is a heating film attached to the outside of the breeding unit, with a power of 50W / layer. It is connected to a temperature controller, with a temperature control accuracy of ±0.5℃. It is equipped with a cooling fan that automatically starts when the temperature exceeds the set value. The ventilation components consist of louvers on both sides of the device, with manually adjustable opening and closing, and an exhaust fan at the top with a power of 15W, set to run for 15 minutes every 2 hours to maintain air circulation. Monitoring module: Includes layered temperature and humidity sensors, one of which is placed in each breeding unit, with an accuracy of ±0.2℃ for temperature and ±2% RH for humidity, and a camera, one of which is located on the top of the device, to observe the activity status of Burmese worms in real time. Sensor data and camera images can be wirelessly transmitted to the terminal to achieve remote monitoring.

[0038] In step 2, the artificial propagation substrate consists of the following components: 50 parts leaf mold, 25 parts decomposed mulberry branch powder, 20 parts peat moss, 8 parts perlite, 3 parts compound microbial inoculant, 2 parts sucrose, and 1 part calcium carbonate.

[0039] Step 2, the preparation method includes the following steps: Step 2.1: Raw material pretreatment Mix leaf mold, decomposed mulberry branch powder, peat moss and perlite, and stir well to obtain the basic substrate. Step 2.2: Microbial Agent and Nutrient Addition Add the compound microbial agent, sucrose and calcium carbonate to the base matrix while stirring at 150 r / min for 15 minutes. Step 2.3: Humidity Adjustment Add deionized water to the mixed substrate, adjust the substrate humidity to 70%, then put the substrate into the breeding unit, adjust the substrate pH to 7.2, lay it to a thickness of 6cm, and let it stand for 24 hours before use.

[0040] In step 2.1, the leaf mold was collected from broad-leaved forests and crushed to a particle size of ≤5mm. The decomposed mulberry branch powder was made by crushing mulberry branches to a particle size of ≤3mm after high-temperature decomposition for 3 months. The pH of the peat soil was adjusted to 7.0. The perlite was sieved through a 5mm sieve to remove impurities. In step 2.2, the compound microbial agent contains Bacillus subtilis, Trichoderma harzianum, Streptomyces cerevisiae, and Nocardia asteroides in a concentration ratio of 2:3:3:2, with an effective viable count ≥5×10⁻⁶. 8 CFU / g.

[0041] In step 3, adjustments will be made in stages according to the following conditions: Phase 1: Adult release and mating stage: (1) Release density: Select healthy adults with a body length of 1.2cm and a female-to-male ratio of 1:1, and release them at a density of 10 individuals / m². 2 The density was applied to the substrate surface of the breeding unit; (2) Environmental parameters: temperature controlled at 25℃, humidity controlled at 80%, and light intensity at 12h light / 12h darkness; (3) Feeding management: Feed the insects with special feed once a day, with the amount being 8% of the total weight of the adult insects. Clean up any leftover feed 24 hours after feeding to prevent mold growth. (4) Mating observation: Observe the behavior of the female insect through the camera. If the female insect is lying still and milky white spots can be seen on the ventral cyst, the mating is successful. Calculate the number of insects that have successfully mated and calculate the mating rate.

[0042] Second stage: Egg hatching stage (1) Substrate adjustment: Transfer the female insects that have successfully mated to a new breeding unit, leave 200 eggs on the surface of the substrate in the breeding unit, and cover them with a 2cm thick layer of sterile decaying leaves; (2) Environmental parameters: Temperature increased to 28℃, humidity maintained at 80%, and lighting adjusted to complete darkness; (3) Hatching monitoring: Check the temperature and humidity data through the terminal every day. If the eggs crack, white larvae crawl out of the eggshell, and the larvae can move normally (crawling, extending appendages), it is judged as successful hatching; if the eggs are shriveled, moldy, or the embryos turn black and die, it is judged as unhatched; if no new larvae hatch for 3 consecutive days, the hatching process is judged to be over. Record the number of successfully hatched larvae, the egg hatching cycle, and the weight of 100 eggs. Third stage: Larval rearing stage (1) Density control: Larvae hatched within 48 hours should be promptly transferred to other breeding units and bred at a density of 200 larvae / m². 2 To avoid excessive larval density leading to competition for nutrients and affecting insect development; (2) Environmental parameters: Temperature controlled at 26℃, humidity reduced to 75%, and light intensity restored to 12h light / 12h darkness; (3) Feeding Management: When the larvae are less than 0.3 cm in length, i.e., 1-15 days after hatching, feed them diluted microbial solution once a day; when the larvae are more than 0.4 cm in length, i.e., after 15 days of hatching, add decomposed mulberry branch chips (≤1 mm in diameter) to the microbial solution once a day, per m 2 Feed 100g to avoid competition for nutrients among the larvae; (4) Substrate replacement: Replace the surface substrate every 10 days with a thickness of 2cm and replenish with newly prepared breeding substrate to avoid depletion of substrate nutrients or accumulation of harmful substances; (5) Observation of larval survival rate: From the day the larvae hatch, observe once a day at 9 o'clock until the larvae's abdominal limbs appear bristle-like or swollen. Record the number of healthy larvae that can crawl flexibly and whose appendages can extend normally. Calculate the larval survival rate, larval stage, and weight of 100 larvae.

[0043] Fourth stage: Adult maturation stage (1) Adult maturation: When the abdominal limbs of the insect appear bristle-like or swollen, that is, when the body length is 1.0-1.2cm and the body color is gray or blackish-gray, transfer it to the maturation and breeding unit. The breeding unit is covered with a 3cm thick substrate of sterile perlite and leaf mold in a 1:1 ratio, with a humidity of 65%, a temperature of 25℃, a humidity of 70%, and a light exposure of 10h light / 14h darkness. (2) Collection of breeding insects: Collect adult male and female insects with blackish-gray body color using an insect suction device. The female insects should be no less than 1.2 cm in length and the male insects should be no less than 1.0 cm in length. They should be retained at a ratio of 2:1 for females and males. The rest are used for experiments or ecological applications.

[0044] In the first stage (2), the atomizing nozzle sprays once every 4 hours for 10 seconds each time, using a 40W LED cold light lamp to avoid direct strong light; In the first stage (2), the special feed is prepared by weight as follows: 15 parts cuttlebone powder, 30 parts wheat bran, 25 parts fermented soybean meal, 5 parts yeast powder, and 0.5 parts vitamin C, mixed and crushed to a particle size ≤1mm; In the second stage (1), the sterile decaying leaves were sterilized at 121℃ for 30 minutes under high pressure, while maintaining a humidity of 70%; In the second stage (2), the atomizing nozzle sprays once every 6 hours, for 8 seconds each time; In the third stage (2), the atomizing nozzle sprays once every 8 hours for 5 seconds each time, and the LED light brightness is adjusted to 100 lux to simulate a natural low light environment; In the third stage (3), the microbial inoculum is a compound microbial agent diluted with water at a ratio of 1:50, per m 2 Spray 500mL; In the fourth stage (1), a 3cm thick substrate of sterile perlite, leaf mold and decomposed mulberry branches was laid in the breeding unit of the maturation unit, with a ratio of 1:1:1 and a humidity of 65%.

[0045] The measurement index method is as follows: Mating rate: Using a camera or stereomicroscope, the behavior and ventral morphology of female insects were observed daily at 9:00 AM. A female insect was considered to have successfully mated when its movement decreased and milky-white spots appeared in its abdominal cysts. The cumulative number of successfully mated females was recorded. Mating rate (%) = Cumulative number of successfully mated females * 100% / Number of female insects tested Weight of 100 eggs: Weigh the plastic box containing the eggs (M1) using an electronic analytical balance with an accuracy of 0.001g, then weigh the gross weight of the plastic box (M2), and count the number of eggs (N) inside the plastic box. Weight of 100 eggs = (M1 - M2) * 100% / N 3. Egg Stage Measurement: Place the plastic box containing the eggs in a constant temperature and humidity incubator. Observe twice daily, at 9:00 AM and 5:00 PM, recording the morphological changes of the eggs (e.g., egg color changing from milky white to light brown), until the larvae hatch and the time is recorded as D2. Simultaneously record the number of successfully hatched eggs. The egg stage of a single egg = hatching date D2 - oviposition date D1.

[0046] 4. Hatching rate (%) = (Number of hatched larvae / Total number of eggs) × 100% 5. Larval stage determination: Immediately after hatching, larvae were transferred to larval rearing boxes using a soft brush, one larva per box, with 30 larvae per treatment. The changes in the abdominal segments of the larvae were observed daily, and the time when the abdominal segments grew setae or began to swell was recorded (D3). Larval stage = D3 - D2.

[0047] 6. Larval survival rate: Starting from the day of hatching, larvae were observed once daily at 9:00 AM until the abdominal appendages showed bristle-like or swollen appearance. The number of healthy larvae that could crawl flexibly and whose appendages could extend normally was recorded. Larval survival rate (%) = Number of healthy larvae * 100% / Total number of larvae tested 7. Methods for measuring larval weight Larvae within 24 hours of hatching were selected for measurement. An electronic analytical balance with an accuracy of 0.001g was used for weighing. 100 larvae were measured in each group, and the measurements were repeated 3 times. The average value was calculated.

[0048] 8. Adult stage determination Mature adults were reared individually, their survival status was observed daily, and their time of death (D4) was recorded. Adult stage = D4 - D3 9. Methods for measuring the body length of adult insects Select adult insects whose abdominal limbs have developed bristle-like or swollen appearance within 24 hours. Dip a brush in water and place the insect on a damp piece of oiled paper. Adjust the insect's body to a naturally straight position with the brush. Scan the insect using a scanner and read its body length using software. Measure 20 insects per group and calculate the average value.

[0049] 10. Methods for measuring adult insect weight Adult insects with bristle-like or swollen abdominal limbs within 24 hours were selected. 100 insects were selected for each treatment and weighed using an electronic analytical balance with an accuracy of 0.001 g. Each treatment was repeated 3 times, and the average value was calculated.

[0050] Comparative Example 1: Adult release and mating stage: Select healthy adults with a body length of 1.2cm and a female-to-male ratio of 1:1, and place them in plastic culture dishes with a diameter of 20cm and a height of 10cm. The bottom of the culture dish is covered with a 2cm thick layer of sterilized moist humus soil and fallen leaves (humidity 80%, able to clump together when squeezed but not crumble when released). Place the entire culture dish in a constant temperature and humidity incubator, setting the temperature at 25℃, the humidity at 80%, and the light cycle at 12h light / 12h darkness. Feed the adults with fallen leaves and humus soil every 2 days, with the amount of feed being 8% of the total weight of the adults. Observe the behavior of the female insects through a camera. When the female insects are dormant and milky white spots are visible on their ventral cysts, mating is considered successful. Calculate the number of insects that successfully mated and calculate the mating rate.

[0051] Egg hatching: Select 200 healthy eggs of uniform size and bright color and place them in a sterile culture dish with a layer of moistened absorbent cotton at the bottom. Cover the dish and transfer it to a constant temperature and humidity incubator (temperature 22℃, humidity 75%). Check the temperature and humidity data daily through the terminal. If the eggs crack and white larvae crawl out of the eggshell and can move normally (crawling, extending appendages), they are considered to have hatched successfully. If the eggs are shriveled, moldy, or the embryos turn black and die, they are considered to have not hatched. If no new larvae hatch for 3 consecutive days, the hatching process is considered to be over. Record the number of successfully hatched larvae, the egg hatching cycle, and the weight of 100 eggs. Larval culture: Select 300 healthy newly hatched Burmese mulberry larvae from the same batch with hatching times within 24 hours of each other. Place them in plastic petri dishes with a diameter of 35cm and a height of 8cm. Cover the bottom with a 2cm layer of sterile, moist humus soil and fallen leaf debris (the soil should be moist enough to clump together when squeezed but not crumble when released). Place the plastic petri dishes in a constant temperature and humidity incubator (temperature 25℃, relative humidity 80%). Feed them daily with a mixture of sterile mulberry leaf debris and humus, with the amount of food just enough to cover the bottom of the petri dish. Clean up any uneaten food and larval excrement promptly. Observe the soil moisture daily. If the surface is dry, add a small amount of sterile water with a pipette to keep it moist. Do not allow water to accumulate. Observe the larvae once a day at 9 am from the day of hatching until the abdominal limbs of the larvae appear bristle-like or swollen. Record the number of healthy larvae that crawl flexibly, have normally extended appendages, and calculate the larval survival rate, larval stage, and weight of 100 larvae.

[0052] Adult maturation stage Select 200 adult insects whose abdominal limbs have developed bristle-like or swollen appearance within 24 hours and place them in plastic petri dishes with a diameter of 35 cm and a height of 8 cm. The bottom of each dish should be covered with a 2 cm layer of sterile, moist humus soil and fallen leaf debris (the soil should be moist enough to clump together when squeezed but not crumble when released). Place the petri dishes in a constant temperature and humidity incubator (temperature 25℃, relative humidity 80%). Feed the insects daily with a mixture of sterile mulberry leaf debris and humus, just enough to cover the bottom of the dish. Promptly remove any uneaten food and larval excrement. Observe the soil moisture daily; if the surface is dry, add a small amount of sterile water using a pipette to maintain moisture, but avoid water accumulation. Observe the insects' behavior every morning and record the time of death until all insects have died. During this period, measure the insect weight, adult cycle, and body length.

[0053] The measurement methods for each indicator are as described in Examples 1, 2 and 3.

[0054] Results: The developmental status of each stage of *Burnus burmannii* under the artificial propagation methods of Examples 1-3 and the comparative example (i.e., the conventional method) of this invention is shown in the table. The hatching rates of *Burnus burmannii* eggs in Examples 1-3 were 90.94%, 92.23%, and 93.19%, respectively, which was more than 46.39% higher than the 62.12% in the comparative example; the egg stages were 7.23 days, 7.46 days, and 6.48 days, respectively, which was more than 29.90% shorter than the 11.74 days in the comparative example; the weight of 100 eggs was not significantly different from the comparative example; the larval development periods were 40.23 days, 38.92 days, and 36.12 days, respectively, which was significantly shorter than the comparative example. The larval growth period was 58.11 days, significantly shorter than the control group by 30.77%; the larval weight was higher than the control group, but the difference was not significant; the larval survival rate reached 96.17%, 98.22%, and 97.16%, which was more than 41.76% higher than the control group; the adult body length and weight were not significantly different from the control group; the adult mating rate reached 85.94%, 88.16%, and 89.45%, which was 14.23% higher than the control group's 75.23%.

[0055] Table 1. Developmental parameters of each stage of *Burmaea molluscica*

[0056] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the technical scope of the present invention. Therefore, any minor modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims

1. A method for artificial propagation of Burmese worms, characterized in that, Includes the following steps: Step 1: Construct artificial breeding equipment The artificial breeding device includes a multi-layer breeding unit, an environmental control module, and a monitoring module. Step 2: Preparation of artificial breeding substrate The composition of the artificial breeding substrate is determined, the raw materials are pretreated first, then microbial agents and nutrients are added, and finally the humidity is adjusted. Step 3: Control of artificial breeding conditions The above-mentioned device and substrate were used to artificially propagate Burmese worms, and the process was carried out in stages.

2. The method for artificial propagation of Burmese worms according to claim 1, characterized in that, In step 1, the multi-layer breeding unit, environmental control module, and monitoring module have the following specific structures: Multi-layer breeding unit: Made of transparent acrylic material, the overall structure is a cuboid with a length of 80cm × width of 50cm × height of 10cm per layer, with a total of 3-5 layers and a layer spacing of 30cm; each layer of the breeding unit has 5-8 water-permeable holes with a diameter of 2cm at the bottom, and the holes are covered with 100-mesh nylon mesh. A water-receiving tray with a height of 3cm is placed below the water-permeable holes; the top of the inner wall of the breeding unit has a ring of anti-escape protrusions, 5cm wide, inclined inward at 45°, and made of polytetrafluoroethylene. Environmental control module: including humidity control components, temperature control components and ventilation components; The humidity control component is a layered atomizing nozzle, with two nozzles at the top of each breeding unit. It is connected to a programmable timer, which can set the spray frequency. It is equipped with a 10L water tank and a micro water pump with a head of ≥2m. The temperature regulation component is a heating film attached to the outside of the breeding unit, with a power of 50W / layer, connected to a temperature controller, with a temperature control accuracy of ±0.5℃, and equipped with a cooling fan that automatically starts when the temperature exceeds the set value. The ventilation components are louvers on both sides of the device, with manually adjustable opening and closing, and an exhaust fan at the top with a power of 15W, set to run for 15 minutes every 2 hours. Monitoring module: Includes layered temperature and humidity sensors, one of which is placed in each breeding unit, with an accuracy of ±0.2℃ for temperature and ±2% RH for humidity, and a camera, one of which is located on the top of the device, to observe the activity status of Burmese worms in real time. Sensor data and camera images can be wirelessly transmitted to the terminal.

3. The method for artificial propagation of Burmese worms according to claim 1, characterized in that, In step 2, the artificial propagation substrate consists of the following components: 40-50 parts leaf mold, 20-25 parts decomposed mulberry branch powder, 15-20 parts peat moss, 5-8 parts perlite, 2-3 parts compound microbial agent, 1-2 parts sucrose, and 0.5-1 parts calcium carbonate.

4. The method for artificial propagation of Burmese worms according to claim 1, characterized in that, Step 2, the preparation method includes the following steps: Step 2.1: Raw material pretreatment Mix leaf mold, decomposed mulberry branch powder, peat moss and perlite, and stir well to obtain the basic substrate. Step 2.2: Microbial Agent and Nutrient Addition Add the compound microbial agent, sucrose and calcium carbonate to the base matrix while stirring at 150 r / min for 10-15 minutes. Step 2.3: Humidity Adjustment Add deionized water to the mixed substrate and adjust the substrate humidity to 65%-70%. Then, put the substrate into the breeding unit, adjust the substrate pH to 6.8-7.2, and lay it to a thickness of 5-6 cm. Let it stand for 24 hours before use.

5. The method for artificial propagation of Burmese worms according to claim 4, characterized in that, In step 2.1, the leaf mold is collected from broad-leaved forests and crushed to a particle size of ≤5mm. The decomposed mulberry branch powder is made by crushing mulberry branches to a particle size of ≤3mm after high-temperature decomposition for 3 months. The pH of the peat soil is adjusted to 6.5-7.

0. The perlite is sieved through a 5mm sieve to remove impurities. In step 2.2, the compound microbial agent contains Bacillus subtilis, Trichoderma harzianum, Streptomyces cerevisiae, and Nocardia asteroides in a concentration ratio of 2:3:3:2, with an effective viable count ≥5×10⁻⁶. 8 CFU / g.

6. The method for artificial propagation of *Burmaea brevis* according to claim 1, characterized in that, In step 3, the control is implemented in stages according to the following conditions: Phase 1: Adult release and mating stage: (1) Release density: Select healthy adults with a body length of 1.0-1.2cm and a female-to-male ratio of 1:1, and release them at a density of 9-12 individuals / m². 2 The density was applied to the substrate surface of the breeding unit; (2) Environmental parameters: temperature controlled at 22-25℃, humidity controlled at 75%-80%, and light intensity at 12h light / 12h darkness; (3) Feeding management: Feed the insects with special feed once a day, with the amount being 5%-8% of the total weight of the adults. Clean up any leftover feed 24 hours after feeding. (4) Mating rate observation: Observe the insect body through the camera. If the female insect body is dormant and milky white spots can be seen on the ventral cyst body, the mating is successful. Calculate the number of insects that have successfully mated and calculate the mating rate. Second stage: Egg hatching stage (1) Substrate adjustment: Transfer the female insects that have successfully mated to a new breeding unit, and cover the surface of the original breeding unit substrate with a 2cm thick layer of sterile decaying leaves; (2) Environmental parameters: Temperature increased to 25-28℃, humidity maintained at 75%-80%, and lighting adjusted to complete darkness; (3) Hatching monitoring: Check the temperature and humidity data through the terminal every day, and sample the substrate every 3 days. If the eggs crack, white larvae crawl out of the egg shell and the larvae can move normally, it is judged as successful hatching; if the eggs are shriveled, moldy, or the embryos turn black and die, it is judged as unhatched; if no new larvae hatch for 3 consecutive days, the hatching process is judged to be over. Record the number of successfully hatched larvae, the egg hatching cycle and the weight of 100 eggs. Third stage: Larval rearing stage (1) Density control: Larvae hatched within 24 hours should be promptly transferred to other breeding units, with a breeding density of 200-250 larvae / m². 2 ; (2) Environmental parameters: Temperature is controlled at 23-26℃, humidity is reduced to 70%-75%, and light intensity is restored to 12h light / 12h darkness; (3) Feeding Management: When the larvae are less than 0.3 cm in length, i.e., 1-15 days after hatching, feed them diluted microbial solution once a day; when the larvae are more than 0.4 cm in length, i.e., after 15 days of hatching, add decomposed mulberry branch chips (≤1 mm in diameter) once a day to the microbial solution, per m 2 Feed 100g; (4) Substrate replacement: Replace the surface substrate every 10 days with a thickness of 2cm and replenish with newly prepared breeding substrate; (5) Observation of larval survival rate: From the day the larvae hatch, observe once a day at 9 o'clock until the larvae's abdominal limbs appear bristle-like or swollen. Record the number of larvae that crawl flexibly, whose appendages can extend normally, and that are healthy. Calculate the larval survival rate, larval stage, and weight of 100 larvae. Fourth stage: Adult maturation stage (1) Adult maturation: When the abdominal limbs of the insect appear bristle-like or swollen, that is, when the body length is 1.0-1.2cm and the body color is gray or blackish-gray, transfer it to the maturation and breeding unit. The breeding unit is covered with a 3cm thick substrate of sterile perlite and leaf mold in a 1:1 ratio, with a humidity of 65%, a temperature controlled at 24-25℃, a humidity of 70%, and a light exposure of 10h light / 14h darkness. (2) Collection of breeding insects: Collect adult male and female insects with blackish-gray body color using an insect suction device. The female insects should be no less than 1.2 cm in length and the male insects should be no less than 1.0 cm in length. Keep them in a male-to-female ratio of 2:

1. The rest are used for experiments or ecological applications.

7. The method for artificial propagation of Burmese worms according to claim 6, characterized in that, In the first stage (2), the atomizing nozzle sprays once every 4 hours for 10 seconds each time, using a 40W LED cold light lamp to avoid direct strong light; In the first stage (2), the special feed is mixed and crushed to a particle size ≤1mm by weight as follows: 15 parts cuttlebone powder, 30 parts wheat bran, 25 parts fermented soybean meal, 5 parts yeast powder, and 0.5 parts vitamin C. In the second stage (1), sterile decaying leaves are sterilized at 121°C for 30 minutes under high pressure while maintaining a humidity of 70%; In the second stage (2), the atomizing nozzle sprays once every 6 hours, for 8 seconds each time; In the third stage (2), the atomizing nozzle sprays once every 8 hours for 5 seconds each time, and the LED light brightness is adjusted to 100 lux to simulate a natural low light environment; In the third stage (3), the microbial inoculum is a compound microbial agent diluted with water at a ratio of 1:50, per m 2 Spray 500mL; In the fourth stage (1), a 3cm thick substrate of sterile perlite, leaf mold and decomposed mulberry branches is laid in the breeding unit of the maturation unit, with a ratio of 1:1:1 and a humidity of 65%.

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