Agricultural plant growth regulator based on refined bamboo vinegar and preparation method thereof
By combining refined bamboo vinegar with compound additives, compound additives, surfactants, and stabilizers, the problems of low utilization rate and unstable effect of the effective components of bamboo vinegar growth regulators are solved, achieving efficient utilization of components and long-lasting and stable growth-promoting and disease-resistant effects.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-27
- Publication Date
- 2026-03-13
AI Technical Summary
Existing bamboo vinegar-based growth regulators have low utilization rates of active ingredients, limited functionality, and unstable effects.
By combining refined bamboo vinegar with compound additives, compound additives, surfactants and stabilizers, including activated carbon treatment to remove impurities, compound additives to supplement trace elements, and attapulgite as a carrier to improve dispersibility, the synergistic effect of the components is achieved.
It improves the utilization rate and functional diversity of active ingredients, resulting in long-lasting and stable effects, avoiding rapid loss and degradation of active ingredients, and prolonging the effects of promoting growth and fighting disease.
Smart Images
Figure CN121647280A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of organic agriculture technology, specifically to a method for preparing an agricultural plant growth regulator based on refined bamboo vinegar. Background Technology
[0002] Bamboo vinegar is a byproduct of the pyrolysis process of bamboo. It contains a variety of natural organic components such as organic acids, phenols, and alcohols. Studies have shown that it has a variety of biological activities, such as promoting plant growth, broad-spectrum antibacterial activity, repelling pests, and inducing plants to develop stress resistance. It shows good application prospects in green agriculture.
[0003] In existing technologies, bamboo vinegar-based plant growth regulators often use crude bamboo vinegar directly or are simply compounded with a single nutrient element, resulting in low utilization of effective components and limited functionality. Therefore, this invention provides an agricultural plant growth regulator based on refined bamboo vinegar and its preparation method. Summary of the Invention
[0004] The purpose of this invention is to provide an agricultural plant growth regulator based on refined bamboo vinegar and its preparation method. The agricultural plant growth regulator based on refined bamboo vinegar prepared by this invention effectively improves the utilization rate of active ingredients, has diverse functions, and has a long-lasting and stable effect.
[0005] To achieve the above objectives, the present invention provides the following technical solution: In a first aspect, the present invention provides a method for preparing an agricultural plant growth regulator based on refined bamboo vinegar, comprising the following steps: S1: Prepare refined bamboo vinegar liquid, compound additive liquid and compound additive for later use; S2: Add refined bamboo vinegar, plant-derived growth promoter and compound additive to a mixing tank, stir at 30℃ and 300rpm for 30min to obtain the initial mixture; S3: Add surfactant, stabilizer and compound additive to the initial mixture, stir at 500 rpm for 1 hour, then adjust the pH to 3-4 with 8-10% citric acid solution, filter with 300 mesh filter cloth to obtain agricultural plant growth regulator based on refined bamboo vinegar liquid.
[0006] Furthermore, the mass ratio of the refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant, and stabilizer is (65-75):(5-10):(4-7):(3-6):(2-4):(1-2).
[0007] Furthermore, the refined bamboo vinegar is obtained by treating bamboo vinegar with activated carbon.
[0008] Furthermore, the composite additive solution is prepared by mixing zinc sulfate, borax and ammonium molybdate.
[0009] Furthermore, the composite additive is prepared by mixing powder and attapulgite.
[0010] Furthermore, the attapulgite clay is pretreated before preparing the composite additive.
[0011] Furthermore, the preparation method of the refined bamboo vinegar is as follows: bamboo vinegar is passed through an activated carbon adsorption bed at a flow rate of 1-2 BV / h, the effluent is collected, activated carbon powder is added to the effluent, stirred at 30°C for 30 min, and filtered to obtain refined bamboo vinegar. The mass of activated carbon powder is 0.1-0.3% of the mass of the effluent, and the particle size of activated carbon powder is 25-75 μm.
[0012] Further, the preparation method of the composite additive solution is as follows: zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of (8-12):(2-4):1, disodium ethylenediaminetetraacetate is added, and the mixture is stirred at 60°C and 300 rpm for 2 hours. The pH is adjusted to neutral, and the composite additive solution is obtained after filtration. The mass of disodium ethylenediaminetetraacetate is 1-2 times the total mass of zinc sulfate, borax and ammonium molybdate.
[0013] Furthermore, the preparation method of the composite additive is as follows: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:(4-6) to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1: (9-11) to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 10-20 min, collect the solid, freeze dry at -50°C and 10-20 Pa vacuum for 12-24 h to obtain the composite additive.
[0014] Furthermore, the method for preparing the powder is as follows: a. Add Bacillus licheniformis freeze-dried powder to deionized water at a mass ratio of 1:(95-105), shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 1-2 h to obtain an enzymatic hydrolysate, wherein the mass of lysozyme is 1-2% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 10-20 min, collect the supernatant, add ammonium sulfate to the supernatant to saturation of 50%, let stand for 8 h, centrifuge at 12,000 rpm for 10-20 min, and collect the precipitate. Step b is carried out at 4℃. c. The precipitate is reconstituted with deionized water at a mass ratio of 1:(7-9), stirred at 37℃ and 500rpm for 10-20min to obtain a reconstituted solution. The reconstituted solution is dialyzed at 4℃ for 12h and freeze-dried at -40℃ under vacuum of 10Pa for 12h to obtain a powder.
[0015] Further, the pretreatment method of the attapulgite is as follows: attapulgite is mixed with deionized water at a mass ratio of 1:(8-12), ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 10-20 min to collect the supernatant, acetic acid is slowly added to the supernatant, stirred at 200 rpm and 40℃ for 1-2 h, centrifuged at 10000 rpm for 10-20 min, the solid is collected, vacuum dried at 60℃ to constant weight, and ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the attapulgite particle size is 50-100 nm, and the mass of acetic acid is 8-12% of the mass of attapulgite.
[0016] Furthermore, the plant-derived growth promoter is alginate oligosaccharide.
[0017] Furthermore, the surfactant is tea saponin.
[0018] Furthermore, the stabilizer is sodium citrate.
[0019] Compared with the prior art, the beneficial effects of the present invention are: 1. In this invention, bamboo vinegar is treated with activated carbon to remove tar, pigments, and inhibitory impurities, enriching it with highly active organic acids, phenols, and other effective components. The compound additive replenishes the trace elements missing in the soil, and disodium EDTA chelates zinc, boron, and molybdenum trace elements, preventing soil fixation of trace elements. It also works synergistically with bamboo vinegar to locally acidify the soil and promote the dissolution and absorption of trace elements. The powder in the compound additive works synergistically with bamboo vinegar to activate defense pathways. Pretreated attapulgite soil is used as a carrier to improve the dispersibility of the regulator. Each component effectively improves the utilization rate of the effective components and expands the function of the regulator.
[0020] 2. In this invention, the bamboo vinegar liquid has higher purity and stronger stability after being treated with activated carbon, avoiding the degradation problem of active ingredients caused by impurities in crude bamboo vinegar liquid. The chelating structure of the compound additive liquid makes it difficult for trace elements such as zinc, boron and molybdenum to precipitate in the soil, and maintains an absorbable state for a long time. The pretreated attapulgite soil serves as a natural slow-release carrier, which efficiently adsorbs bioactive ingredients and realizes the slow release of active substances. This avoids the problem of short-lived effects caused by rapid nutrient loss and instantaneous degradation of active ingredients in traditional regulators, making the regulator's effect lasting and stable. Attached Figure Description
[0021] Figure 1 The flowchart presents an agricultural plant growth regulator based on refined bamboo vinegar and its preparation method. Detailed Implementation
[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.
[0023] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings.
[0024] A method for preparing an agricultural plant growth regulator based on refined bamboo vinegar includes the following steps: S1: Prepare refined bamboo vinegar liquid, compound additive liquid and compound additive for later use; S2: Add refined bamboo vinegar, plant-derived growth promoter and compound additive to a mixing tank, stir at 30℃ and 300rpm for 30min to obtain the initial mixture; S3: Add surfactant, stabilizer and compound additive to the initial mixture, stir at 500 rpm for 1 hour, then adjust the pH to 3-4 with 8-10% citric acid solution, filter with 300 mesh filter cloth to obtain agricultural plant growth regulator based on refined bamboo vinegar liquid.
[0025] In this embodiment, the mass ratio of refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant, and stabilizer is (65-75):(5-10):(4-7):(3-6):(2-4):(1-2).
[0026] In this embodiment, the refined bamboo vinegar is obtained by treating bamboo vinegar with activated carbon.
[0027] In this embodiment, the composite additive solution is prepared by mixing zinc sulfate, borax and ammonium molybdate.
[0028] In this embodiment, the composite additive is prepared by mixing powder and attapulgite clay.
[0029] In this embodiment, attapulgite clay is pretreated before the preparation of the composite additive.
[0030] In this embodiment, the preparation method of refined bamboo vinegar is as follows: bamboo vinegar is passed through an activated carbon adsorption bed at a flow rate of 1-2 BV / h, the effluent is collected, activated carbon powder is added to the effluent, stirred at 30°C for 30 min, and filtered to obtain refined bamboo vinegar. The mass of activated carbon powder is 0.1-0.3% of the mass of the effluent, and the particle size of activated carbon powder is 25-75 μm.
[0031] In this embodiment, the preparation method of the composite additive solution is as follows: zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of (8-12):(2-4):1, disodium ethylenediaminetetraacetate is added, and the mixture is stirred at 60°C and 300 rpm for 2 hours. The pH is adjusted to neutral, and the composite additive solution is obtained after filtration. The mass of disodium ethylenediaminetetraacetate is 1-2 times the total mass of zinc sulfate, borax and ammonium molybdate.
[0032] In this embodiment, the preparation method of the composite additive is as follows: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:(4-6) to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1: (9-11) to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 10-20 min, collect the solid, freeze dry at -50°C and 10-20 Pa vacuum for 12-24 h to obtain the composite additive.
[0033] In this embodiment, the powder is prepared as follows: a. Add Bacillus licheniformis freeze-dried powder to deionized water at a mass ratio of 1:(95-105), shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 1-2 h to obtain an enzymatic hydrolysate, wherein the mass of lysozyme is 1-2% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 10-20 min, collect the supernatant, add ammonium sulfate to the supernatant to saturation of 50%, let stand for 8 h, centrifuge at 12,000 rpm for 10-20 min, and collect the precipitate. Step b is carried out at 4℃. c. The precipitate is reconstituted with deionized water at a mass ratio of 1:(7-9), stirred at 37℃ and 500rpm for 10-20min to obtain a reconstituted solution. The reconstituted solution is dialyzed at 4℃ for 12h and freeze-dried at -40℃ under vacuum of 10Pa for 12h to obtain a powder.
[0034] In this embodiment, the pretreatment method of attapulgite is as follows: attapulgite and deionized water are mixed at a mass ratio of 1:(8-12), ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 10-20 min to collect the supernatant, acetic acid is slowly added to the supernatant, stirred at 200 rpm and 40℃ for 1-2 h, centrifuged at 10000 rpm for 10-20 min, the solid is collected, vacuum dried at 60℃ to constant weight, and ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the attapulgite particle size is 50-100 nm, and the mass of acetic acid is 8-12% of the mass of attapulgite.
[0035] In this embodiment, the plant-derived growth promoter is alginate oligosaccharide.
[0036] In this embodiment, the surfactant is tea saponin.
[0037] In this embodiment, the stabilizer is sodium citrate.
[0038] Based on the foregoing embodiments, the inventors also conducted the following sets of experiments: It should be noted that all raw materials used in the following experiments are commercially available.
[0039] Experiment 1: Preparation of refined bamboo vinegar: Bamboo vinegar is passed through an activated carbon adsorption bed at a flow rate of 1 BV / h, the effluent is collected, activated carbon powder is added to the effluent, stirred at 30℃ for 30 min, and filtered to obtain refined bamboo vinegar. The mass of activated carbon powder is 0.1% of the mass of the effluent, and the particle size of activated carbon powder is 25 μm.
[0040] Preparation of the composite additive solution: Zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of 8:2:1, and disodium ethylenediaminetetraacetate is added. The mixture is stirred at 60℃ and 300 rpm for 2 hours, and the pH is adjusted to neutral. After filtration, the composite additive solution is obtained, wherein the mass of disodium ethylenediaminetetraacetate is 1 times the total mass of zinc sulfate, borax and ammonium molybdate.
[0041] Pretreated attapulgite: Attapulgite and deionized water were mixed at a mass ratio of 1:8, ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 10 min, and the supernatant was collected. Acetic acid was slowly added to the supernatant, and the mixture was stirred at 200 rpm and 40℃ for 1 h. After centrifugation at 10000 rpm for 10 min, the solid was collected and vacuum dried at 60℃ to constant weight. The solid was then ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the particle size of the attapulgite was 50 nm, and the mass of acetic acid was 8% of the mass of the attapulgite.
[0042] Preparation of powder: a. Add Bacillus licheniformis freeze-dried powder to deionized water at a mass ratio of 1:95, shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 1 h to obtain an enzymatic hydrolysate. The mass of lysozyme is 1% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 10 min, collect the supernatant, add ammonium sulfate to the supernatant until saturation is 50%, let stand for 8 h, centrifuge at 12,000 rpm for 10 min, and collect the precipitate. Step b is carried out at 4 °C. c. The precipitate was reconstituted with deionized water at a mass ratio of 1:7, stirred at 37°C and 500 rpm for 10 min to obtain a reconstituted solution. The reconstituted solution was dialyzed at 4°C for 12 h and then freeze-dried at -40°C under vacuum of 10 Pa for 12 h to obtain a powder.
[0043] Preparation of composite additives: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:4 to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1:9 to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 10 min, collect the solid, freeze dry at 10 Pa vacuum and -50°C for 12 h to obtain the composite additive.
[0044] Preparation of agricultural plant growth regulator based on refined bamboo vinegar: Refined bamboo vinegar, plant-derived growth promoter and compound additive were added to a stirred tank and stirred at 30°C and 300 rpm for 30 min to obtain a preliminary mixture. Surfactant, stabilizer and compound additive were added to the preliminary mixture and stirred at 500 rpm for 1 h. The pH was then adjusted to 3 with 8% citric acid solution and filtered through a 300-mesh filter cloth to obtain the agricultural plant growth regulator based on refined bamboo vinegar. The mass ratio of refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant and stabilizer was 65:5:4:3:2:1.
[0045] Experiment 2: Preparation of refined bamboo vinegar: Bamboo vinegar was passed through an activated carbon adsorption bed at a flow rate of 1.5 BV / h, the effluent was collected, activated carbon powder was added to the effluent, stirred at 30℃ for 30 min, and filtered to obtain refined bamboo vinegar. The mass of activated carbon powder was 0.2% of the mass of the effluent, and the particle size of the activated carbon powder was 50 μm.
[0046] Preparation of the composite additive solution: Zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of 10:3:1, and disodium ethylenediaminetetraacetate is added. The mixture is stirred at 60℃ and 300 rpm for 2 hours, and the pH is adjusted to neutral. After filtration, the composite additive solution is obtained, wherein the mass of disodium ethylenediaminetetraacetate is 1.5 times the total mass of zinc sulfate, borax and ammonium molybdate.
[0047] Pretreated attapulgite: Attapulgite and deionized water were mixed at a mass ratio of 1:10, ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 15 min, and the supernatant was collected. Acetic acid was slowly added to the supernatant, and the mixture was stirred at 200 rpm and 40℃ for 1.5 h. After centrifugation at 10000 rpm for 15 min, the solid was collected and vacuum dried at 60℃ to constant weight. The solid was then ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the particle size of the attapulgite was 75 nm, and the mass of acetic acid was 10% of the mass of the attapulgite.
[0048] Preparation of powder: a. Add Bacillus licheniformis lyophilized powder to deionized water at a mass ratio of 1:100, shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 1.5 h to obtain an enzymatic hydrolysate, wherein the mass of lysozyme is 1.5% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 15 min, collect the supernatant, add ammonium sulfate to the supernatant to saturation of 50%, let stand for 8 h, centrifuge at 12,000 rpm for 15 min, and collect the precipitate. Step b is carried out at 4 °C. c. The precipitate was reconstituted with deionized water at a mass ratio of 1:8, and stirred at 37°C and 500 rpm for 15 min to obtain a reconstituted solution. The reconstituted solution was dialyzed at 4°C for 12 h, and then freeze-dried at -40°C under vacuum of 10 Pa for 12 h to obtain a powder.
[0049] Preparation of composite additives: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:5 to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1:10 to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 15 min, collect the solid, freeze dry at 15 Pa and -50°C for 18 h to obtain the composite additive.
[0050] Preparation of agricultural plant growth regulator based on refined bamboo vinegar: Refined bamboo vinegar, plant-derived growth promoter and compound additive were added to a stirred tank and stirred at 30°C and 300 rpm for 30 min to obtain a preliminary mixture. Surfactant, stabilizer and compound additive were added to the preliminary mixture and stirred at 500 rpm for 1 h. The pH was then adjusted to 3.5 with 9% citric acid solution and filtered through a 300-mesh filter cloth to obtain the agricultural plant growth regulator based on refined bamboo vinegar. The mass ratio of refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant and stabilizer was 70:8:5.5:4.5:3:1.5.
[0051] Experiment 3: Preparation of refined bamboo vinegar: Bamboo vinegar was passed through an activated carbon adsorption bed at a flow rate of 2 BV / h, the effluent was collected, activated carbon powder was added to the effluent, stirred at 30℃ for 30 min, and filtered to obtain refined bamboo vinegar. The mass of activated carbon powder was 0.3% of the mass of the effluent, and the particle size of the activated carbon powder was 75 μm.
[0052] Preparation of the composite additive solution: Zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of 12:4:1, and disodium ethylenediaminetetraacetate is added. The mixture is stirred at 60℃ and 300 rpm for 2 hours, and the pH is adjusted to neutral. After filtration, the composite additive solution is obtained, wherein the mass of disodium ethylenediaminetetraacetate is twice the total mass of zinc sulfate, borax and ammonium molybdate.
[0053] Pretreated attapulgite: Attapulgite and deionized water were mixed at a mass ratio of 1:12, ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 20 min, and the supernatant was collected. Acetic acid was slowly added to the supernatant, and the mixture was stirred at 200 rpm and 40℃ for 2 h. After centrifugation at 10000 rpm for 20 min, the solid was collected, vacuum dried at 60℃ to constant weight, and ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the attapulgite particle size was 100 nm and the mass of acetic acid was 12% of the mass of attapulgite.
[0054] Preparation of powder: a. Add Bacillus licheniformis freeze-dried powder to deionized water at a mass ratio of 1:105, shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 2 h to obtain an enzymatic hydrolysate, wherein the mass of lysozyme is 2% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 20 min, collect the supernatant, add ammonium sulfate to the supernatant to saturation of 50%, let stand for 8 h, centrifuge at 12,000 rpm for 20 min, and collect the precipitate. Step b is carried out at 4 °C. c. The precipitate was reconstituted with deionized water at a mass ratio of 1:9, and stirred at 37°C and 500 rpm for 20 min to obtain a reconstituted solution. The reconstituted solution was dialyzed at 4°C for 12 h and then freeze-dried at -40°C under vacuum of 10 Pa for 12 h to obtain a powder.
[0055] Preparation of composite additives: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:6 to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1:11 to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 20 min, collect the solid matter, freeze dry at 20 Pa and -50°C for 24 h to obtain the composite additive.
[0056] Preparation of agricultural plant growth regulator based on refined bamboo vinegar: Refined bamboo vinegar, plant-derived growth promoter and compound additive were added to a stirred tank and stirred at 30°C and 300 rpm for 30 min to obtain a preliminary mixture. Surfactant, stabilizer and compound additive were added to the preliminary mixture and stirred at 500 rpm for 1 h. The pH was then adjusted to 4 with 10% citric acid solution and filtered through a 300-mesh filter cloth to obtain the agricultural plant growth regulator based on refined bamboo vinegar. The mass ratio of refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant and stabilizer was 75:10:7:6:4:2.
[0057] Comparative Example 1: The difference between this comparative example and Experiment 1 is as follows: In this comparative example, the bamboo vinegar was not treated with activated carbon.
[0058] Comparative Example 2: The difference between this comparative example and Experiment 1 is as follows: This comparative example does not contain any compound additives.
[0059] Comparative Example 3: The difference between this comparative example and Experiment 1 is that: This comparative example does not contain any composite additives.
[0060] Comparative Example 4: The difference between this comparative example and Experiment 1 is as follows: The composite additives in this comparative example do not contain attapulgite.
[0061] Performance testing: The agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiments 1, 2, 3, Comparative Examples 1, 2, 3, and 4 were subjected to performance testing. The test data are recorded in the table below: Table 1 Growth Status Test Table
[0062] Table 2 Zinc, Boron, and Molybdenum Content Test Table
[0063] Table 3. Incidence Rate Test Table
[0064] In performance testing, the temperature was maintained at 25°C during the day and 15°C at night, with a humidity of 60%. Tomato seedlings with uniform growth at the three-leaf-one-heart stage were selected and transplanted into standard culture pots. Each treatment group was sprayed with the same dose of the sample growth regulator. The height growth of the tomato seedlings was measured 10 days and 20 days after spraying. The higher the growth length, the better the growth promotion effect. The stability and persistence of the growth promotion effect were measured by comparing the two data. Soil samples were taken from standard culture pots, and the total content of trace elements zinc, boron and molybdenum in the soil of each treatment group was tested by spectrophotometry. The proportion of available / fixed boron and molybdenum in the soil after 10 days was tested by NY / T 1121. The test results reflect the stability and persistence of trace elements in the soil. Tomato seedlings with uniform growth at the three-leaf-one-heart stage were selected, transplanted into standard culture pots, and sprayed with an equal amount of bacterial wilt fungicide. Multiple parallel experiments were set up, with each treatment group sprayed with the corresponding regulator at the same dosage. The incidence rate of each group was counted at 10 days and 20 days. The lower the incidence rate, the stronger the disease resistance.
[0065] The 10-day growth length test results of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were 8.2cm, 8.5cm, 8.8cm, 6.9cm, 7.6cm, 7.8cm and 8.0cm, respectively.
[0066] The 20-day growth length test results of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were 12.9cm, 13.2cm, 13.5cm, 10.7cm, 12.2cm, 11.9cm and 11.5cm, respectively.
[0067] The total zinc, boron, and molybdenum content of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiments 1, 2, 3, Comparative Examples 1, 2, 3, and 4 were 12.5 mg / kg, 13.0 mg / kg, 13.2 mg / kg, 12.3 mg / kg, 9.9 mg / kg, 12.0 mg / kg, and 12.1 mg / kg, respectively.
[0068] The percentage of boron available in the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 was 37.9%, 38.2%, 38.7%, 36.2%, 30.1%, 33.4% and 33.6% respectively after 10 days.
[0069] The percentage of molybdenum available in the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 after 10 days was 30.8%, 31.2%, 31.7%, 29.5%, 22.7%, 25.5% and 25.6%, respectively.
[0070] The 10-day disease incidence rates of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4 were 13.1%, 12.3%, 11.5%, 39.0%, 14.2%, 45.1%, and 14.5%, respectively.
[0071] The 20-day disease incidence rates of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Experiment 1, Experiment 2, Experiment 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4 were 28.3%, 27.2%, 26.8%, 80.7%, 30.0%, 88.3%, and 45.2%, respectively.
[0072] As shown in Tables 1, 2, and 3, the growth-promoting effect, disease resistance, trace element content, and long-term stability of the agricultural plant growth regulators based on refined bamboo vinegar prepared in Comparative Examples 1, 2, 3, and 4 were all lower than those in Experiments 1, 2, and 3. This indicates that Comparative Example 1 verified that activated carbon treatment can remove inhibitory impurities such as tar and pigments from bamboo vinegar, enrich active ingredients such as organic acids and phenols, and improve growth-promoting efficiency. The growth indicators of Comparative Examples 2, 3, and 4 were all lower than those of the experimental group, indicating that the zinc, boron, and molybdenum provided by the compound additive are essential nutrients for plants. Attapulgite, as a natural carrier, can improve the dispersibility of the regulator and prevent the active ingredients from agglomerating and becoming ineffective. Comparing the growth data from 10 to 20 days, the change rates of the data in Comparative Examples 1-4 were 50.7%, 52.6%, 46.1%, and 48.5%, respectively, indicating that pre-treatment of attapulgite allows for the slow release of active ingredients in the powder, which is a key raw material for extending the growth-promoting cycle. The experimental group had higher levels of zinc, boron, and molybdenum than the blank control and comparative example 2. This is because the compound additive directly supplements trace elements, while comparative example 2 does not contain this component, hence its lower total content. After 10 days, the experimental group showed a significantly higher proportion of boron / molybdenum in the available state than the comparative example. This indicates that the chelating effect of disodium EDTA on trace elements effectively prevents soil fixation of trace elements. Furthermore, the local acidification effect of refined bamboo vinegar promotes dissolution and absorption. At the same time, the stabilizing effect of attapulgite soil on trace elements delays their conversion to the fixed state. This suggests that the compound additive not only provides a large number of essential trace elements for plants but also effectively prolongs the free period of trace elements. Other components also slightly improve the effective utilization of trace elements by promoting dissolution and absorption and by dispersing and stabilizing. Comparative Examples 1 and 3 showed extremely high incidence rates, indicating that impurities in the crude bamboo vinegar reduced the activity of antibacterial components such as phenols. The Bacillus licheniformis contained in the compound additive powder, after treatment, produced Bacillus licheniformis capsid proteins that could recognize chitin receptors on the surface of plant roots, activate the salicylic acid signaling pathway, induce the expression of disease-related proteins, thicken the cell wall, and produce phytoalexins. This synergistic antibacterial effect with the bamboo vinegar resulted in a significant increase in the incidence rate without the compound additive. Comparative Example 4, lacking attapulgite, caused the antibacterial components in the powder to easily aggregate and become inactive, resulting in a significant increase in the incidence rate after 20 days. This also indicates that the regulator can maintain disease resistance for a long time, thanks to the bamboo vinegar being pretreated to remove impurities and the pretreated attapulgite in the compound additive acting as a carrier to adsorb the antibacterial components and assist in their slow release.
[0073] By comparing and analyzing the relevant data in the table, it can be seen that the agricultural plant growth regulator based on refined bamboo vinegar prepared by this invention not only effectively improves the utilization rate of active ingredients but also has good long-lasting stability. This indicates that the agricultural plant growth regulator based on refined bamboo vinegar and its preparation method provided by this invention have a broader market prospect and are more suitable for promotion.
[0074] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0075] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims
1. A method for preparing an agricultural plant growth regulator based on refined bamboo vinegar, characterized in that, Includes the following steps: S1: Prepare refined bamboo vinegar liquid, compound additive liquid and compound additive for later use; S2: Add refined bamboo vinegar, plant-derived growth promoter and compound additive to a mixing tank, stir at 30℃ and 300rpm for 30min to obtain the initial mixture; S3: Add surfactant, stabilizer and compound additive to the initial mixture, stir at 500 rpm for 1 hour, then adjust the pH to 3-4 with 8-10% citric acid solution, filter with 300 mesh filter cloth to obtain agricultural plant growth regulator based on refined bamboo vinegar liquid. The mass ratio of the refined bamboo vinegar, plant-derived growth promoter, compound additive, compound additive, surfactant, and stabilizer is (65-75):(5-10):(4-7):(3-6):(2-4):(1-2); The refined bamboo vinegar is obtained by treating bamboo vinegar with activated carbon. The composite additive solution is prepared by mixing zinc sulfate, borax and ammonium molybdate. The composite additive is prepared by mixing powder and attapulgite clay; The attapulgite clay is pretreated before the preparation of the composite additive.
2. The method for preparing the agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The method for preparing the refined bamboo vinegar is as follows: bamboo vinegar is passed through an activated carbon adsorption bed at a flow rate of 1-2 BV / h, the effluent is collected, activated carbon powder is added to the effluent, the mixture is stirred at 30°C for 30 min, and then filtered to obtain the refined bamboo vinegar. The mass of the activated carbon powder is 0.1-0.3% of the mass of the effluent, and the particle size of the activated carbon powder is 25-75 μm.
3. The method for preparing the agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The preparation method of the composite additive solution is as follows: zinc sulfate, borax and ammonium molybdate are mixed in a mass ratio of (8-12):(2-4):1, disodium ethylenediaminetetraacetate is added, and the mixture is stirred at 60°C and 300 rpm for 2 hours. The pH is adjusted to neutral, and the composite additive solution is obtained after filtration. The mass of disodium ethylenediaminetetraacetate is 1-2 times the total mass of zinc sulfate, borax and ammonium molybdate.
4. The method for preparing the agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The preparation method of the composite additive is as follows: (1) Dissolve the powder in Tris-HCl buffer solution at pH=8 at a solid-liquid ratio of 1:(4-6) to obtain the first mixture; (2) Disperse the pretreated attapulgite in deionized water at a ratio of 1: (9-11) to obtain a second mixture; (3) Add the first mixture to the second mixture slowly at a volume ratio of 1:1, stir at 30°C and 500 rpm for 1 h, centrifuge at 8000 rpm for 10-20 min, collect the solid, freeze dry at -50°C and 10-20 Pa vacuum for 12-24 h to obtain the composite additive.
5. The method for preparing an agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The preparation method of the powder is as follows: a. Add Bacillus licheniformis freeze-dried powder to deionized water at a mass ratio of 1:(95-105), shake at 200 rpm and 37°C for 30 min to obtain a bacterial suspension. Add lysozyme to the bacterial suspension and enzymatically hydrolyze at 37°C for 1-2 h to obtain an enzymatic hydrolysate, wherein the mass of lysozyme is 1-2% of the mass of the bacterial suspension. b. Centrifuge the enzymatic hydrolysate at 10,000 rpm for 10-20 min, collect the supernatant, add ammonium sulfate to the supernatant to saturation of 50%, let stand for 8 h, centrifuge at 12,000 rpm for 10-20 min, and collect the precipitate. Step b is carried out at 4℃. c. The precipitate is reconstituted with deionized water at a mass ratio of 1:(7-9), stirred at 37℃ and 500rpm for 10-20min to obtain a reconstituted solution. The reconstituted solution is dialyzed at 4℃ for 12h and freeze-dried at -40℃ under vacuum of 10Pa for 12h to obtain a powder.
6. The method for preparing the agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The pretreatment method for attapulgite is as follows: Attapulgite and deionized water are mixed at a mass ratio of 1:(8-12), ultrasonically dispersed for 30 min, centrifuged at 10000 rpm for 10-20 min, and the supernatant is collected. Acetic acid is slowly added to the supernatant, stirred at 200 rpm and 40℃ for 1-2 h, centrifuged at 10000 rpm for 10-20 min, the solid is collected, vacuum dried at 60℃ to constant weight, and ground through a 100-mesh sieve to obtain pretreated attapulgite, wherein the particle size of attapulgite is 50-100 nm, and the mass of acetic acid is 8-12% of the mass of attapulgite.
7. The method for preparing an agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The plant-derived growth promoter is alginate oligosaccharide.
8. The method for preparing the agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The surfactant is tea saponin.
9. The method for preparing an agricultural plant growth regulator based on refined bamboo vinegar according to claim 1, characterized in that, The stabilizer is sodium citrate.
10. An agricultural plant growth regulator based on refined bamboo vinegar, characterized in that, It is prepared by the method for preparing agricultural plant growth regulator based on refined bamboo vinegar as described in any one of claims 1-9.