A compound premixed feed specifically for green-shelled laying hens during their egg-laying period and its preparation method.

Abstract

This invention discloses a compound premixed feed specifically for green-shelled laying hens during their egg-laying period and its preparation method. The compound premixed feed provided by this invention includes: green tea, nucleotide residue, dicalcium phosphate, limestone powder, soybean lecithin oil, sodium chloride, heme protein powder, taurine, choline chloride, turmeric, enzyme preparation, phytase, oregano, DL-methionine, amino acid complex, trace element premix, organic mineral complex, vitamin premix, vitamin C, vitamin E, and antioxidants. This invention selects the above components from a large number of existing additives and combines them in specific proportions. The resulting composition can improve the flock's stress resistance, enhance flock stability, and solve the problem of flock collapse; it can also delay the aging of laying hens, improve the production performance and egg quality of green-shelled laying hens during their egg-laying period, thereby effectively improving eggshell color and increasing the product's market value.

Description

Technical Field

[0001] This invention belongs to the field of animal feed technology, specifically relating to a compound premixed feed for green-shelled laying hens during their egg-laying period. Background Technology

[0002] Green-shelled chickens are characterized by producing highly nutritious green eggs. Common breeds include Dongxiang Black Feather Green-shelled Chicken, Suqin Green-shelled Chicken, Lushi Green-shelled Chicken, Changshun Green-shelled Chicken, and Shendan No. 6 Green-shelled Chicken. Compared to regular eggs, green-shelled eggs are rich in lecithin, vitamin A, vitamin E, and trace elements such as zinc, iodine, and selenium. They also contain abundant amino acids, especially lysine, aspartic acid, and glutamic acid. In addition, they have lower cholesterol content, making them more in line with people's needs for a healthy diet.

[0003] However, green-shelled chickens are active and prone to breaking up, which can easily lead to intestinal problems, making management difficult and causing large fluctuations in egg production, thus increasing breeding costs and reducing final profits.

[0004] Furthermore, consumers pay close attention to the shell color of green-shelled eggs, but after peak laying period (after 40 weeks of age), the shell color of green-shelled eggs rapidly fades, severely impacting the market value of the eggs. Currently, solutions to the eggshell color problem mainly focus on brown-shelled hens: supplementing with porphyrin iron to provide protoporphyrin, the main component of eggshell pigment; using nutrients with hematopoietic function, traditional Chinese medicine, and polysaccharides to promote health and pigment synthesis, thereby improving eggshell color. However, unlike brown-shelled hens, the eggshell pigment of green-shelled hens is mainly composed of biliverdin IX and biliverdin zinc chelates, which are twice that of protoporphyrin, and their pigment metabolism pathway also differs significantly from that of brown-shelled hens. In addition, the fading of eggshell color in the later stages of laying in green-shelled hens is mainly related to aging, including increased gene methylation rate and decreased expression of related genes, which in turn causes changes in pigment metabolism.

[0005] In summary, there is an urgent need to address the issues of green-shelled chickens being prone to scattering and the eggshell color lightening in the later stages of egg production. Summary of the Invention

[0006] This invention provides a compound premixed feed (hereinafter referred to as premix) suitable for the laying period of green-shelled chickens. The premix can improve the stress resistance of the flock, enhance the stability of the flock, and solve the problem of easy flock collapse. At the same time, it can also delay the aging of laying hens, improve the production performance and egg quality of green-shelled chickens during the laying period, thereby improving eggshell color in the long term and enhancing the market value of the product.

[0007] To achieve the above objectives, the present invention employs the following technical solution:

[0008] In a first aspect, the present invention provides a compound premixed feed specifically for green-shelled laying hens during their egg-laying period, comprising the following components in parts by weight:

[0009] Green tea, 10.0-20.0 parts by weight.

[0010] Nucleotide residue 10.0-20.0 parts by weight,

[0011] 15.0-25.0 parts by weight of dicalcium phosphate.

[0012] Stone powder 15.0-30.0 parts by weight,

[0013] Soybean lecithin oil 0.5-1.0 parts by weight,

[0014] Sodium chloride 5.0-8.0 parts by weight

[0015] Heme protein powder 2.0-8.0 parts by weight

[0016] Taurine 0.5-2.0 parts by weight,

[0017] Choline chloride 0.5-3.0 parts by weight

[0018] Turmeric 0.5-2.0 parts by weight,

[0019] Enzyme preparation, 0.2-1.0 parts by weight.

[0020] Phytase 0.2-1.0 parts by weight,

[0021] Oreganophenol 0.2-0.8 parts by weight,

[0022] DL-Methionine 2.5-4.0 parts by weight

[0023] Amino acid complex package, 2.0-5.0 parts by weight.

[0024] Trace element premix 2.8-3.2 parts by weight,

[0025] Organic-mineral composite package, 0.5-2.0 parts by weight.

[0026] Vitamin premix 0.6-0.8 parts by weight,

[0027] Vitamin C 0.1-0.5 parts by weight

[0028] Vitamin E 0.02-0.15 parts by weight

[0029] Antioxidant 0.2-0.4 parts by weight.

[0030] In one specific embodiment of the present invention, the compound premixed feed is composed of the following components in parts by weight:

[0031] 10.0-15.0 parts by weight of green tea

[0032] Nucleotide residue 15.0-20.0 parts by weight,

[0033] 15.0-25.0 parts by weight of dicalcium phosphate.

[0034] Stone powder 15.0-30.0 parts by weight,

[0035] Soybean lecithin oil 0.5-0.8 parts by weight,

[0036] Sodium chloride 5.0-8.0 parts by weight

[0037] Heme protein powder 2.0-8.0 parts by weight

[0038] Taurine 0.5-1.0 parts by weight,

[0039] Choline chloride 1.5-3.0 parts by weight

[0040] Turmeric 0.5-1.0 parts by weight,

[0041] Enzyme preparation, 0.2-1.0 parts by weight.

[0042] Phytase 0.2-1.0 parts by weight,

[0043] Oreganophenol 0.2-0.8 parts by weight,

[0044] DL-methionine 2.5-4.0 parts by weight

[0045] Amino acid complex package, 2.0-3.0 parts by weight.

[0046] Trace element premix 2.8-3.2 parts by weight,

[0047] Organic-mineral composite package, 0.5-1.5 parts by weight.

[0048] Vitamin premix 0.6-0.8 parts by weight,

[0049] Vitamin C 0.1-0.2 parts by weight,

[0050] Vitamin E 0.02-0.06 parts by weight,

[0051] Antioxidant 0.3-0.4 parts by weight.

[0052] As a specific embodiment of the present invention, it is composed of the following components in parts by weight:

[0053] 10.0-15.0 parts by weight of green tea

[0054] Nucleotide residue 15.0-20.0 parts by weight,

[0055] 15.0-25.0 parts by weight of dicalcium phosphate.

[0056] Stone powder 15.0-30.0 parts by weight,

[0057] Soybean lecithin oil 0.8-1.0 parts by weight,

[0058] Sodium chloride 5.0-8.0 parts by weight

[0059] Heme protein powder 2.0-8.0 parts by weight

[0060] Taurine 1.0-2.0 parts by weight,

[0061] Choline chloride 0.5-1.5 parts by weight,

[0062] Turmeric 1.0-2.0 parts by weight,

[0063] Enzyme preparation, 0.2-1.0 parts by weight.

[0064] Phytase 0.2-1.0 parts by weight,

[0065] Oreganophenol 0.2-0.8 parts by weight,

[0066] DL-methionine 2.5-4.0 parts by weight

[0067] Amino acid complex package 3.0-5.0 parts by weight,

[0068] Trace element premix 2.8-3.2 parts by weight,

[0069] Organic-mineral composite package, 1.0-2.0 parts by weight.

[0070] Vitamin premix 0.6-0.8 parts by weight,

[0071] Vitamin C 0.1-0.2 parts by weight,

[0072] Vitamin E 0.02-0.06 parts by weight,

[0073] Antioxidant 0.2-0.3 parts by weight.

[0074] As a specific embodiment of the present invention, it is composed of the following components in parts by weight:

[0075] Green tea, 15.0-20.0 parts by weight.

[0076] Nucleotide residue 10.0-15.0 parts by weight,

[0077] 15.0-25.0 parts by weight of dicalcium phosphate.

[0078] Stone powder 15.0-30.0 parts by weight,

[0079] Soybean lecithin oil 0.5-0.8 parts by weight,

[0080] Sodium chloride 5.0-8.0 parts by weight

[0081] Heme protein powder 2.0-8.0 parts by weight

[0082] Taurine 1.0-2.0 parts by weight,

[0083] Choline chloride 0.5-1.5 parts by weight,

[0084] Turmeric 0.5-1.0 parts by weight,

[0085] Enzyme preparation, 0.2-1.0 parts by weight.

[0086] Phytase 0.2-1.0 parts by weight,

[0087] Oreganophenol 0.2-0.8 parts by weight,

[0088] DL-methionine 2.5-4.0 parts by weight

[0089] Amino acid complex package, 2.0-3.0 parts by weight.

[0090] Trace element premix 2.8-3.2 parts by weight,

[0091] Organic-mineral composite package, 1.0-2.0 parts by weight.

[0092] Vitamin premix 0.6-0.8 parts by weight,

[0093] Vitamin C 0.2-0.5 parts by weight,

[0094] Vitamin E 0.06-0.15 parts by weight,

[0095] Antioxidant 0.2-0.3 parts by weight.

[0096] The green tea contains ≥4% tea polyphenols;

[0097] The soybean phospholipid oil contains ≥95% lecithin, ≥20% phosphatidylcholine, and ≥20% phosphatidylethanolamine.

[0098] The taurine content is ≥99%;

[0099] The content of choline chloride is ≥50%;

[0100] The enzyme preparation contains protease ≥6000 U / g, amylase ≥500 U / g, xylanase ≥20000 U / g, β-mannanase ≥2000 U / g, β-glucanase ≥3000 U / g, and cellulase ≥500 U / g.

[0101] The phytase activity is ≥10000 U / g;

[0102] The oregano aromaticity contains ≥60% carvacrol;

[0103] The content of DL-methionine is ≥99%;

[0104] The vitamin C content is ≥97%;

[0105] The vitamin E content is ≥50%.

[0106] Apart from the components mentioned above, the purity of the remaining components is the standard purity of feed-grade raw materials.

[0107] In each kilogram of the amino acid complex package, L-threonine ≥ 190 g, L-tryptophan ≥ 142.5 g, L-glycine ≥ 475 g, and L-arginine ≥ 142.5 g.

[0108] In one specific embodiment of the present invention, the amino acid complex is composed of L-threonine, L-tryptophan, L-glycine and L-arginine.

[0109] As a specific embodiment of the present invention, the trace element premix is ​​composed of copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium selenite and calcium iodate.

[0110] As a specific embodiment of the present invention, the organic mineral composite package is composed of hydroxymethionine chelated copper, hydroxymethionine chelated zinc, hydroxymethionine chelated manganese, yeast selenium, and cobalt chloride.

[0111] Each kilogram of the vitamin premix contains: Vitamin A 25-33 million IU, Vitamin D3 10-16.5 million IU, Vitamin E ≥50,000 IU, Vitamin K3 5-16.7 g, Vitamin B1 ≥8 g, Vitamin B2 ≥23 g, Vitamin B6 ≥12.5 g, Vitamin B... 12 ≥67 mg, Biotin ≥0.75 g, Niacin ≥100 g, Folic Acid ≥3.3 g, Pantothenic Acid ≥33 g.

[0112] Secondly, the present invention provides a method for preparing the above-mentioned compound premixed feed, comprising the following steps: first mixing components with a proportion of less than 0.2%, and then mixing with the remaining components; wherein the coefficient of variation of the mixing uniformity must be less than 5%.

[0113] Thirdly, the present invention provides a complete diet specifically for green-shelled laying hens during their egg-laying period, comprising the aforementioned compound premixed feed.

[0114] The complete diet comprises the following components by weight: 60-62 parts corn, 23-25 ​​parts soybean meal, 0.5-1 part soybean oil, 4.5-9.5 parts limestone powder, and 5 parts premix; the premix is ​​the above-mentioned compound premixed feed.

[0115] The complete diet also includes wheat bran; the amount of wheat bran used is 2-3 parts by weight.

[0116] The complete diet provided by this invention has a specific formula that can be adjusted according to different feeding stages.

[0117] As a specific embodiment of the present invention, during the pre-production stage, the complete diet consists of the following components by weight: 62 parts corn, 3 parts wheat bran, 24.5 parts soybean meal, 1 part soybean oil, 4.5 parts limestone powder, and 5 parts premix.

[0118] As a specific embodiment of the present invention, during the peak period, the complete diet consists of the following components by weight: 60 parts corn, 25 parts soybean meal, 1 part soybean oil, 9 parts limestone powder, and 5 parts premix.

[0119] As a specific embodiment of the present invention, during the lower peak period, the complete diet consists of the following components by weight: 60.5 parts corn, 24 parts soybean meal, 1 part soybean oil, 9.5 parts limestone powder, and 5 parts premix.

[0120] As a specific embodiment of the present invention, during the pre-production stage, the complete diet consists of the following components by weight: 60 parts corn, 2 parts wheat bran, 23 parts soybean meal, 0.5 parts soybean oil, 9.5 parts limestone powder, and 5 parts premix.

[0121] Fourthly, the present invention provides a method for preparing the complete diet, comprising the following steps:

[0122] (1) Prepare premixed materials with a mixing uniformity variation coefficient of less than 5%;

[0123] (2) Crush and sieve the remaining raw materials and mix them with the premix; the coefficient of variation of uniformity is less than 7%.

[0124] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0125] 1. This invention selects vitamin C to reduce the negative impact of environmental factors on the metabolic process of laying hens and enhance the body's immunity; selects taurine to inhibit neuronal excitation and relieve stress; selects turmeric to downregulate the level of oxidative stress in the body and exert a protective effect against liver damage, thereby improving disease resistance and stress resistance and maintaining the health of the flock.

[0126] 2. This invention selects oregano phenol and utilizes the antibacterial properties of its 30 natural components to optimize the intestinal environment and maintain the intestinal health of laying hens; through the combined use of enzyme preparations and phytase, a multi-enzyme synergistic effect is formed to promote the digestion and absorption of nutrients, thereby improving the production performance of the flock.

[0127] 3. This invention, based on the differences in metabolic pathways related to green-shelled eggshell color, selects raw materials such as L-tryptophan, L-arginine, L-glycine, L-threonine, taurine, soybean lecithin oil, and nucleotide residue to precisely regulate amino acid metabolic pathways, taurine and alpha-taurine metabolic pathways, glycerophospholipid metabolic pathways, and purine metabolic pathways related to green-shelled egg coloring, thereby promoting the formation of a dark green eggshell. Heme protein powder is used to provide porphyrin iron required for eggshell pigment synthesis; cobalt chloride is used to induce heme oxygenase, promoting heme metabolism and biliverdin synthesis, thus improving egg pigment deposition. Hydroxymethionine chelated zinc, hydroxymethionine chelated copper, and hydroxymethionine chelated manganese are selected to improve eggshell strength and quality.

[0128] 4. Since the lightening of eggshell color in the later stages of green-shelled chicken development is mainly due to gene methylation and decreased gene expression during aging, this invention supplements vitamin E to inhibit lipid peroxidation; uses taurine to remove excess reactive oxygen species, neutralize hypochlorous acid produced by neutrophils, improve mitochondrial function, activate the Nrf2 / HO-1 antioxidant defense system pathway, alleviate oxidative stress in chickens, and delay ovarian decline; uses yeast selenium to form selenoproteins in the body, regulating the antioxidant function of chickens; uses green tea rich in tea polyphenols to scavenge free radicals, regulate the activity of antioxidant enzymes and the expression of protein kinase-related genes, reduce oxidative stress damage, and also enhance the delicate texture and unique flavor of eggs; and uses turmeric rich in curcumin, which also has antioxidant and anti-inflammatory effects, thus effectively delaying the aging of green-shelled chickens.

[0129] In summary, this invention selects the above components from a large number of existing additive ingredients and combines them in a specific ratio. The resulting composition, as an additive, can significantly improve the stress resistance of chicken flocks, enhance flock stability, and reduce the risk of disease; improve intestinal health, increase nutrient digestibility, and improve flock production performance; maintain oviduct health, delay aging of laying hens, and improve eggshell quality; precisely supplement the differentiated nutrients in the nutritional metabolism of green-shelled laying hens, regulate the synthesis and decomposition of pigments, and promote pigment deposition in the eggshell. It can not only improve the production performance and egg quality of green-shelled laying hens during the laying period, but also improve eggshell color in the long term and enhance the market value of the product. Detailed Implementation

[0130] The present invention will be further described below with reference to specific embodiments, but the present invention is not limited to the following embodiments.

[0131] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0132] Unless otherwise specified, all reagents, materials, instruments, etc. used in the following examples are commercially available.

[0133] The raw materials selected in this invention fully comply with the national "Feed Raw Material Catalog" and "Additive Variety Catalog". No medicated feed additives are used, and the amount of nutritional feed additives used complies with the nutritional requirements and maximum limits stipulated in Announcement No. 2625.

[0134] Raw material screening test

[0135] Based on the growth characteristics of green-shelled chickens and existing experience, this invention selects nucleotide residue, soybean phospholipid oil, amino acid complex, heme protein powder and organic mineral complex to participate in regulating the metabolic pathways related to the color of green-shelled chicken shells; and selects oregano phenol, utilizing its antibacterial properties to help maintain the intestinal and oviduct health of green-shelled chickens.

[0136] The raw material screening test of this invention mainly focuses on the effect of the premix on improving the antioxidant capacity of green-shelled laying hens.

[0137] The materials used in the following experiments were sourced from:

[0138] 1. Trace element premix: Prepared by the company itself.

[0139] Table 1. Trace element premix composition

[0140]

[0141] 2. Vitamin premix: Prepared by the company itself.

[0142] Table 2 Vitamin Premix Ingredients

[0143]

[0144] 3. Amino acid complex package: prepared in-house.

[0145] Table 3. Ingredients of the Amino Acid Complex

[0146]

[0147] 4. Organic mineral composite package: prepared by the company itself.

[0148] Table 4. Composition of Organic Mineral Composite Pack

[0149]

[0150] I. Large-scale raw material screening test

[0151] 1. Experimental Design

[0152] The experiment used 192 26-week-old Shendan No. 6 green-shelled chickens. The flock was randomly divided into 3 groups of 64 chickens each, and fed control group A, experimental group B, and experimental group C, respectively. The experiment lasted for 4 weeks, and the composition of the basal diet was as follows.

[0153] Table 5 Composition of the basal diet

[0154]

[0155]

[0156] 2. Test Results

[0157] Table 6. Test Results

[0158]

[0159] The results show that the mortality rate of green-shelled chickens in experimental group B (Shendan 6) was lower than that in control group A and experimental group C, suggesting that green tea can reduce the mortality rate of green-shelled chickens. The sensory evaluation score of eggs from experimental group B was higher than that of control group A and experimental group C, indicating that adding 0.5% Sichuan pepper seed meal to the feed did not improve egg flavor, while adding 0.5% green tea could improve egg flavor.

[0160] In addition, during the storage of feed, it was found that the feed in experimental group C had a distinct odor, while the feed in control group A and experimental group B had normal sensory properties. The possible reason is that the trace elements in the premix promoted the oxidative rancidity of unsaturated fatty acids in pepper seed meal and also destroyed the activity of fat-soluble vitamins such as vitamin A.

[0161] In conclusion, green tea is more suitable to be added to this composition than pepper seed meal.

[0162] II. Screening Tests for Small-Scale Raw Materials and Additives

[0163] 1. Experimental Design

[0164] The experiment selected 256 30-week-old Shendan No. 6 green-shelled laying hens. The flock was randomly divided into 4 groups of 64 hens each, and fed control group A, experimental group B, experimental group C, and experimental group D, respectively. The experiment lasted for 4 weeks, and the composition of the basal diet was as follows.

[0165] Table 7 Composition of the basal diet

[0166]

[0167]

[0168] 2. Test Results

[0169] Table 8 Test Results

[0170]

[0171] The results above show that the serum antioxidant capacity and egg quality of experimental groups B, C, and D were all superior to those of control group A, and the results of experimental group D were significantly better than those of experimental groups B and C. This suggests that the addition of turmeric, vitamin C, and vitamin E to this composition has a synergistic effect on improving the antioxidant capacity of green-shelled laying hens.

[0172] Example

[0173] Conventional premixed feeds and compound premixed feed compositions of Examples 1-3

[0174] The recipe is as follows:

[0175] Table 9 Compound Premixed Feed Compositions

[0176]

[0177]

[0178] The specific steps for preparing the above-mentioned compound premixed feed are as follows: first, mix the components with a proportion of less than 0.2%, and then mix them with the remaining components; wherein the coefficient of variation of the mixing uniformity must be less than 5%.

[0179] Application Trial

[0180] I. Application Effects on Suqin Green-shelled Egg Chickens

[0181] 1. Experimental Design

[0182] The experiment selected 19,200 29-week-old Suqin green-shelled chickens. The flock was randomly divided into 4 groups of 4,800 chickens each, and fed control group A (conventional premix), experimental group B (Example 1), experimental group C (Example 2), and experimental group D (Example 3), respectively. The experiment lasted for 4 weeks (June 15, 2024 - July 14, 2024). The composition of the basal diet is as follows.

[0183] Table 10 Composition of the basal diet

[0184]

[0185] 2. Test Results

[0186] Table 11 Test Results

[0187]

[0188] Note:

[0189] Eggshell strength: Eggshell strength was measured using an eggshell strength tester.

[0190] Eggshell color L Value: Measured using a general-purpose colorimeter, L The value represents the brightness of a color, L. =0 represents pure black (darkest), L =100 represents pure white (brightest).

[0191] Eggshell color a Value: Measured using a general-purpose colorimeter, a The value indicates the position of the color between red and green. Positive values ​​indicate red tones, and negative values ​​indicate green tones. The larger the absolute value, the more obvious the color tendency.

[0192] Eggshell color b Value: Measured using a general-purpose colorimeter, b The value indicates the position of the color between yellow and blue. Positive values ​​indicate a yellow tone, and negative values ​​indicate a blue tone. The larger the absolute value, the more obvious the color tendency.

[0193] Eggshell gloss: The gloss of the equatorial region of the eggshell was measured using a gloss meter.

[0194] Egg flavor sensory rating: Consumers are asked to evaluate boiled eggs (whole eggs) based on their aroma, texture, and taste, and rate them on a scale of 0-5, where 5 represents the best flavor and 0 represents the worst flavor.

[0195] The results above show that using Examples 1 (Experimental Group B), 2 (Experimental Group C), and 3 (Experimental Group D) of the present invention can all improve the egg production rate and mortality rate of Suqin green-shelled laying hens under heat stress. Among them, Experimental Group C showed the best effect, followed by Experimental Group B. This proves that Experimental Group C is superior to the conventional approach in terms of disease resistance, stress resistance, and improvement in production performance.

[0196] Regarding eggshell quality, experimental groups B, C, and D all showed significant improvements in eggshell gloss, with group C showing the best effect, followed by group D. Eggshells in group C had a more vibrant color, while the other groups showed slight improvements, but the differences were not significant, possibly related to the better physiological state of the flock during peak egg production. Although group D did not have the optimal production performance, its eggs had a significantly better flavor, possibly due to improved antioxidant capacity and higher tea polyphenol content.

[0197] II. Application Effects on Shendan No. 6 Green-shelled Egg Chickens

[0198] 1. Experimental Design

[0199] The experiment used 32,000 36-week-old Shendan No. 6 green-shelled chickens. The flock was randomly divided into 4 groups of 8,000 chickens each, and fed control group A (conventional premix), experimental group B (Example 1), experimental group C (Example 2), and experimental group D (Example 3), respectively. The experiment lasted for 8 weeks, and the composition of the basal diet was as follows.

[0200] Table 12 Composition of the basal diet

[0201]

[0202] 2. Test Results

[0203] Table 13 Test Results

[0204]

[0205] The results above show that after using Examples 1 (Experimental Group B), 2 (Experimental Group C), and 3 (Experimental Group D) of the present invention, the egg production rate was significantly improved, and the feed conversion ratio and mortality rate were better than those of the control group A. Experimental groups C and D showed the most significant improvement in eggshell color, followed by experimental group B, both of which were better than control group A, proving that the compositions of Examples 1-3 can alleviate the lightening of eggshell color during the middle of egg production. In terms of eggshell gloss and sensory evaluation of egg flavor, experimental group D showed the best results. Experimental group C had the best overall improvement effect, experimental group B had better egg production performance, and experimental group D showed the best improvement in egg flavor.

[0206] III. Application Effects on Dongxiang Green-Shelled Chickens

[0207] 1. Experimental Design

[0208] The experiment used 26,000 50-week-old Dongxiang green-shelled chickens. The flock was randomly divided into 4 groups of 6,500 chickens each, and fed control group A (conventional premix), experimental group B (Example 1), experimental group C (Example 2), and experimental group D (Example 3), respectively. The experiment lasted for 8 weeks, and the composition of the basal diet was as follows.

[0209] Table 14 Composition of the basal diet

[0210]

[0211] 2. Test Results

[0212] Table 15 Test Results

[0213]

[0214] The results above show that after using Examples 1 (Experimental Group B), 2 (Experimental Group C), and 3 (Experimental Group D) of the present invention, the mortality rate of Dongxiang green-shelled laying hens in the later stages of egg production was lower, the eggshell gloss was stronger, and the eggshell strength was slightly improved. The eggshell color of Experimental Groups B, C, and D was darker than that of Control Group A, with Experimental Group C showing the most significant improvement in eggshell color, proving that Examples 1-3 can alleviate the lightening of eggshell color in the later stages of egg production. Experimental Group D showed a more significant effect on improving egg flavor.

[0215] IV. Summary of the Usage Effects of the Examples

[0216] As can be seen from the above results, after using Examples 1-3 of the present invention, Suqin Green-shelled Egg Chicken, Shendan No. 6, and Dongxiang Green-shelled Egg Chicken all showed effects such as improved stress resistance, reduced mortality rate, stable egg production rate, and reduced feed conversion ratio. Among them, Examples 1 and 2 showed the best results.

[0217] Eggshell color performance was best in flocks fed with Examples 2 and 3, which significantly delayed the fading of eggshell color in the mid-to-late stages of egg production. Improvement of eggshell color was limited in the early stages of egg production. Eggshell color was slightly darker in flocks fed with Example 2, while there was no significant difference in other treatment groups, which is related to the health status of the young chickens.

[0218] Eggshell strength and gloss were improved in chicken flocks fed Examples 1-3, with the eggshell quality being even better in flocks fed Examples 2 and 3.

[0219] The flavor of eggs fed to flocks of Examples 1-3 was improved, with the best results observed in flocks fed to Example 3, which significantly improved egg quality and customer acceptance, thereby increasing the sales value of eggs.

[0220] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A compound premixed feed specifically for green-shelled laying hens during their egg-laying period, characterized in that, It consists of the following components in parts by weight: 10.0-15.0 parts by weight of green tea Nucleotide residue 15.0-20.0 parts by weight, 15.0-25.0 parts by weight of dicalcium phosphate. Stone powder 15.0-30.0 parts by weight, Soybean lecithin oil 0.8-1.0 parts by weight, Sodium chloride 5.0-8.0 parts by weight Heme protein powder 2.0-8.0 parts by weight Taurine 1.0-2.0 parts by weight, Choline chloride 0.5-1.5 parts by weight, Turmeric 1.0-2.0 parts by weight, Enzyme preparation, 0.2-1.0 parts by weight. Phytase 0.2-1.0 parts by weight, Oreganophenol 0.2-0.8 parts by weight, DL-methionine 2.5-4.0 parts by weight Amino acid complex package 3.0-5.0 parts by weight, Trace element premix 2.8-3.2 parts by weight, Organic-mineral composite package, 1.0-2.0 parts by weight. Vitamin premix 0.6-0.8 parts by weight, Vitamin C 0.1-0.2 parts by weight, Vitamin E 0.02-0.06 parts by weight Antioxidant 0.2-0.3 parts by weight; The amino acid complex consists of L-threonine, L-tryptophan, L-glycine and L-arginine; The organic mineral composite package consists of hydroxymethionine chelated copper, hydroxymethionine chelated zinc, hydroxymethionine chelated manganese, yeast selenium, and cobalt chloride. The enzyme preparation contains protease ≥ 6000 U / g, amylase ≥ 500 U / g, xylanase ≥ 20000 U / g, β-mannanase ≥ 2000 U / g, β-glucanase ≥ 3000 U / g, and cellulase ≥ 500 U / g.

2. The compound premixed feed according to claim 1, characterized in that, The green tea contains ≥4% tea polyphenols; The taurine content is ≥99%; The content of choline chloride is ≥50%; The phytase activity is ≥10000 U / g; The oregano aromaticity contains ≥60% carvacrol; The content of DL-methionine is ≥99%; The vitamin C content is ≥97%; The vitamin E content is ≥50%; In each kilogram of the amino acid complex package, L-threonine ≥ 190 g, L-tryptophan ≥ 142.5 g, L-glycine ≥ 475 g, and L-arginine ≥ 142.5 g; Each kilogram of the vitamin premix contains: Vitamin A 25-33 million IU, Vitamin D3 10-16.5 million IU, Vitamin E ≥50,000 IU, Vitamin K3 5-16.7 g, Vitamin B1 ≥8 g, Vitamin B2 ≥23 g, Vitamin B6 ≥12.5 g, Vitamin B... 12 ≥67 mg, Biotin ≥0.75 g, Niacin ≥100 g, Folic Acid ≥3.3 g, Pantothenic Acid ≥33 g.

3. The method for preparing the compound premixed feed according to claim 1 or 2, characterized in that, The process includes the following steps: first, mix the components with a proportion of less than 0.2%, and then mix them with the remaining components; wherein the coefficient of variation of the mixing uniformity must be less than 5%.

4. A complete daily ration specifically for green-shelled laying hens during their egg-laying period, characterized in that, Includes the compound premixed feed as described in claim 1 or 2.

5. The complete diet according to claim 4, characterized in that, It includes the following components by weight: 60-62 parts corn, 23-25 ​​parts soybean meal, 0.5-1 part soybean oil, 4.5-9.5 parts limestone powder, and 5 parts premix; The premix is ​​the compound premixed feed as described in claim 1 or 2.

6. The complete diet according to claim 4, characterized in that, The complete diet also includes bran; The amount of bran used is 2-3 parts by weight.

7. The method for preparing a complete diet according to any one of claims 4-6, characterized in that, Includes the following steps: (1) Prepare premixed materials with a mixing uniformity variation coefficient of less than 5%; (2) Crush and sieve the remaining raw materials and mix them with the premix; the coefficient of variation of the mixing uniformity is less than 7%.

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