Black sesame compound protein powder as well as preparation method and application thereof

Abstract

The invention discloses black sesame compound protein powder as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The black sesame compound protein powder is mainly prepared from black sesame extract microcapsules, whey protein powder, resistant dextrin, probiotics, a natural sweetening agent and spices. The black sesame extract microcapsule is prepared by adopting ultrasonic-assisted ethanol extraction and a sodium alginate-chitosan embedding microencapsulation technology, is rich in active ingredients in black sesame, and has various health effects of resisting oxidation, resisting inflammation and the like. Through an innovative preparation process, all the raw material components are reasonably matched, so that the protein powder not only has high protein content, but also has various health-care functions of maintaining beauty and keeping young, resisting aging, reducing blood fat and the like. The black sesame compound protein powder can be widely applied to the fields of sports nutrition, daily health care, special crowd nutrition support and the like, and the requirements of different consumer groups are met.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, and in particular to a black sesame compound protein powder, its preparation method, and its application. Background Technology

[0002] As people pay increasing attention to health, the demand for functional protein powders that provide comprehensive nutrition and specific health benefits is growing. While there is a wide variety of protein powder products available, there is still significant room for improvement in effectively combining the nutrients of black sesame seeds and fully leveraging their unique health benefits. Black sesame seeds are rich in unsaturated fatty acids, vitamin E, sesamin, and other nutrients, possessing significant antioxidant, anti-inflammatory, and lipid-lowering effects. However, most protein powder products on the market currently fail to fully explore and utilize these potential values ​​of black sesame seeds. Summary of the Invention

[0003] This invention aims to provide a black sesame compound protein powder. Through innovative formula design and preparation process, it combines the nutritional advantages of black sesame with high-quality protein powder, thereby creating a compound protein powder product with unique health functions and high nutritional value, meeting consumers' demand for high-quality, multifunctional protein powder.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] The first aspect of this invention provides a black sesame compound protein powder, comprising the following raw material components in parts by weight:

[0006] Black sesame extract microcapsules (5-15 parts): Prepared using ultrasound-assisted ethanol extraction and microencapsulation technology, these microcapsules are rich in active ingredients from black sesame, such as unsaturated fatty acids, vitamin E, and sesamin, and have various health benefits including antioxidant and anti-inflammatory properties.

[0007] 30-60 servings of whey protein powder: Provides high-quality animal protein, rich in all essential amino acids, especially branched-chain amino acids, which help promote muscle repair and growth and increase basal metabolic rate.

[0008] 10-20 servings of resistant dextrin: As a low-calorie, high-fiber carbohydrate, it helps improve intestinal function, regulate blood sugar levels, increase satiety, and aid in weight loss.

[0009] 1-5 servings of probiotics: Adding specific probiotic strains, such as Lactobacillus acidophilus and Bifidobacterium, helps maintain the balance of the intestinal microecology and enhance immunity.

[0010] 0.05~0.15 parts of natural sweeteners and 0.1~0.5 parts of flavorings: Naturally sourced sweeteners and flavorings are used to enhance the taste and flavor of the product while avoiding the potential health risks of artificial additives.

[0011] Furthermore, the whey protein powder has a protein content of ≥80%, and the resistant dextrin has a dietary fiber content of ≥90%.

[0012] The second aspect of this invention provides a method for preparing black sesame compound protein powder:

[0013] One method includes the preparation of microcapsules of black sesame extract:

[0014] S1. After pretreatment such as washing, drying, and crushing, black sesame seeds are extracted using ultrasound-assisted ethanol extraction to extract their active ingredients. Specific process parameters are: ultrasound power 200~400W, extraction temperature 40~60℃, and extraction time 20~40 minutes. This extraction method effectively improves extraction efficiency and ensures that the active ingredients in the black sesame seeds are fully dissolved in the extract.

[0015] S2. The extract was filtered and concentrated to obtain black sesame extract. Subsequently, the extract was microencapsulated using sodium alginate-chitosan encapsulation technology. The specific steps were as follows: sodium alginate solution (concentration 2wt.%~5wt.%) was mixed evenly with black sesame extract to form solution A; chitosan solution (concentration 1wt.%~3wt.%) was used as solution B.

[0016] S3. Solution A is slowly added dropwise to solution B at a specific flow rate, and the reaction is carried out under certain temperature and stirring conditions, so that sodium alginate and chitosan form stable microcapsule walls on the surface of the extract, encapsulating the active ingredients.

[0017] S4. After the reaction is complete, the product undergoes filtration, washing, and drying to obtain black sesame extract microcapsules. This microencapsulation process effectively protects the active ingredients in the black sesame extract, improves its stability and bioavailability, and prevents oxidation, deterioration, or loss of activity during subsequent processing and storage.

[0018] The second method includes a preparation method for black sesame compound protein powder:

[0019] S1. Accurately weigh each ingredient according to the formula ratio, including black sesame extract microcapsules, whey protein powder, resistant dextrin, probiotics, and natural sweeteners and flavorings.

[0020] S2. Premix whey protein powder with a portion of resistant dextrin (30%~50%) using low-speed shear. Mix for 5~15 minutes at a temperature of 20~40℃ and a shear rate of 100~500 rpm to initially and uniformly mix the protein powder and resistant dextrin, forming a premix. This step helps improve the uniformity and efficiency of subsequent mixing processes, while preventing denaturation or aggregation of the protein powder under high-speed shear.

[0021] S3. Add the black sesame extract microcapsules and the remaining resistant dextrin (50%~70%) to the premix and perform a high-speed shear mixing. Mix for 3~10 minutes at a temperature of 25~45℃ and a shear rate of 500~2000rpm to ensure that the black sesame extract microcapsules and resistant dextrin are evenly dispersed in the protein powder, forming a homogeneous intermediate mixture. High-speed shear mixing effectively breaks up the agglomeration between raw material particles, improves the micro-mixing effect, and ensures the uniform distribution of each component in the protein powder.

[0022] S4. Add probiotics, natural sweeteners, flavorings, and other excipients to the intermediate mixture and perform a second high-speed shear mixing. Mix for 8-15 minutes at a temperature of 30-50℃ and a shear rate of 1000-3000 rpm to ensure all raw material components are thoroughly and evenly mixed, yielding the final black sesame compound protein powder product. This step ensures the uniform dispersion of all excipients in the protein powder, further improving the product's quality and stability.

[0023] The third aspect of this invention provides the application of black sesame compound protein powder in the preparation of nutritional supplements, which are used to improve human nutritional status, enhance immunity, resist oxidation, regulate blood lipids, and improve intestinal function.

[0024] The principle and beneficial effects of this technical solution:

[0025] 1. Sports Nutrition Field: This invention can be used as a nutritional supplement before and after exercise, providing athletes and fitness enthusiasts with high-quality protein that is quickly absorbed, promoting muscle repair and growth, and improving athletic performance and recovery. Simultaneously, the unsaturated fatty acids and antioxidants in black sesame extract help reduce exercise-induced oxidative stress and muscle fatigue, accelerating physical recovery.

[0026] 2. Daily Health Care: This invention is suitable for ordinary consumers as a nutritional supplement to their daily diet, meeting the body's comprehensive needs for nutrients such as protein, dietary fiber, vitamins, and minerals. Its antioxidant, anti-inflammatory, and lipid-lowering effects help prevent chronic diseases, enhance immunity, slow down the aging process, and maintain overall health.

[0027] 3. Nutritional Support for Special Populations: This invention can be used for nutritional support for special populations such as the elderly, vegetarians, and those with weakened constitutions. It provides the elderly with easily absorbed high-quality protein and abundant nutrients to help maintain muscle mass, bone health, and immunity; it meets the nutritional needs of vegetarians for non-animal protein while supplementing certain nutrients that may be lacking in plant-based foods; and it provides comprehensive nutritional support for those with weakened constitutions, promoting physical recovery and improving their health. Attached Figure Description

[0028] Figure 1 This is a flowchart of a preparation method for a black sesame compound protein powder. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the embodiments and accompanying drawings. Here, the illustrative embodiments and descriptions of this invention are used to explain the invention, but are not intended to limit the invention.

[0030] It should also be noted that, in order to avoid obscuring the invention with unnecessary details, only the processing steps closely related to the solution according to the invention are shown in the accompanying drawings, while other details that are not closely related to the invention are omitted.

[0031] It should be emphasized that the term "including / comprises" as used herein refers to the presence of a feature, element, or step, but does not exclude the presence or addition of one or more other features, elements, or steps.

[0032] It should be emphasized here that the step markers mentioned below are not a limitation on the order of the steps, but should be understood as meaning that the steps can be executed in the order mentioned in the embodiments, or in a different order than in the embodiments, or several steps can be executed simultaneously.

[0033] Example 1

[0034] refer to Figure 1 A method for preparing black sesame compound protein powder, comprising:

[0035] Preparation of black sesame extract microcapsules:

[0036] S1. After pretreatment such as washing, drying, and crushing, black sesame seeds are extracted using ultrasound-assisted ethanol extraction to extract their active ingredients. Specific process parameters are: ultrasound power 300W, extraction temperature 50℃, and extraction time 30 minutes. During the extraction process, the cavitation effect of ultrasound effectively breaks down the cell walls of the black sesame seeds, promoting the release and dissolution of active ingredients, thus improving extraction efficiency and yield.

[0037] S2. The extract was filtered and concentrated to obtain black sesame extract. Subsequently, the extract was microencapsulated using sodium alginate-chitosan encapsulation technology. A 3.5 wt.% sodium alginate solution was mixed evenly with the black sesame extract to form solution A; a 2 wt.% chitosan solution was used as solution B.

[0038] S3. Slowly add solution A to solution B at a constant flow rate and react for 30 minutes at 35°C and a stirring rate of 150 rpm to allow sodium alginate and chitosan to form stable microcapsule walls on the surface of the extract.

[0039] S4. After the reaction is complete, the product undergoes filtration, washing, and drying to obtain black sesame extract microcapsules. The microencapsulated black sesame extract exhibits good stability, with an encapsulation rate of over 80% for its active ingredients, effectively preventing oxidation and loss of the active ingredients during subsequent processing and storage.

[0040] Preparation of black sesame compound protein powder:

[0041] S1. Weigh each ingredient according to the following formula ratio: black sesame extract microcapsules: 10 parts, whey protein powder: 50 parts, resistant dextrin: 15 parts, Lactobacillus acidophilus powder: 3 parts, steviol glycosides: 0.1 parts, natural flavoring: 0.2 parts.

[0042] S2. Whey protein powder and 5 parts of resistant dextrin are premixed at low speed under conditions of 30°C and 300 rpm for 10 minutes to form a premix.

[0043] S3. Add the black sesame extract microcapsules and the remaining 10 parts of resistant dextrin to the premix and perform a high-speed shear mixing for 5 minutes at 35°C and a shear rate of 1200 rpm to form a uniform intermediate mixture.

[0044] S4. Finally, add Lactobacillus acidophilus powder, steviol glycosides and natural flavorings to the intermediate mixture, and perform a second high-speed shear mixing for 10 minutes at 40°C and a shear rate of 2000 rpm to obtain the finished black sesame compound protein powder.

[0045] Example 2

[0046] Efficacy verification of black sesame compound protein powder

[0047] Experimental Design: Sixty healthy adults aged 20-45 years were randomly divided into an experimental group and a control group, with 30 participants in each group. The experimental group took 10g of the black sesame compound protein powder prepared in Example 1 daily, 30 minutes after breakfast and dinner, respectively; the control group did not take any protein powder products. All participants maintained normal lifestyles and dietary habits, without any special dietary control or exercise training. The experiment lasted for 12 weeks. Body composition, blood biochemical indicators, and gut microbiota of the participants were tested and evaluated before and after the experiment.

[0048] Detection indicators and methods:

[0049] Body composition analysis: Bioelectrical impedance analysis (BIA) was used to measure the subjects' weight, body fat percentage, lean body mass (i.e., fat-free mass), and other indicators to assess the impact of protein powder on body composition.

[0050] Blood biochemical index testing: Fasting venous blood samples were collected from the subjects to test the serum total protein, albumin, four blood lipids (total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol), superoxide dismutase (SOD, an antioxidant enzyme) activity, malondialdehyde (MDA, an indicator of oxidative stress damage) content, and other indicators to assess the impact of protein powder on nutritional status, antioxidant capacity and blood lipid levels.

[0051] Gut microbiota analysis: Fecal samples were collected from subjects, and high-throughput sequencing technology was used to analyze the composition and diversity of gut microbiota, assessing the impact of protein powder on gut microbiota.

[0052] Experimental results:

[0053] Refer to Table 1 for changes in body composition:

[0054] Experimental group: average weight loss of 2.1 kg (P<0.05), average decrease in body fat percentage of 3.2% (P<0.01), and average increase in lean body mass of 0.8 kg (P<0.05). This indicates that black sesame compound protein powder can help with fat loss while also promoting muscle growth and improving body composition.

[0055] Control group: There were no significant changes in body weight and body fat percentage, but the average increase in lean body mass was 0.2 kg (P>0.05), which may be related to normal physiological fluctuations and daily activities.

[0056] Refer to Table 1 for changes in blood biochemical indicators:

[0057] Nutritional indicators: Serum total protein and albumin levels were elevated in the experimental group, but P>0.05. Protein powder can improve the body's intake and utilization of protein and improve nutritional status, but the changes in these indicators were not significant in non-malnourished patients.

[0058] Antioxidant indicators: The SOD activity in the experimental group was significantly increased (P<0.01), and the MDA content was significantly decreased (P<0.01), indicating that the black sesame compound protein powder has a significant antioxidant effect, which can effectively reduce oxidative stress damage in the body and enhance the body's antioxidant defense capabilities.

[0059] Lipid indicators: The experimental group showed decreased serum total cholesterol and triglyceride levels, increased high-density lipoprotein cholesterol levels, and decreased low-density lipoprotein cholesterol levels. This indicates that protein powder has a positive effect on regulating blood lipid levels and helps reduce the risk of cardiovascular disease.

[0060] Refer to Table 1 for changes in gut microbiota:

[0061] The experimental group showed a significant increase in the number of beneficial bacteria such as Bifidobacteria and Lactobacillus in fecal samples, while a significant decrease in the number of harmful bacteria such as Escherichia coli. Simultaneously, the diversity index of the gut microbiota also increased, indicating that black sesame compound protein powder can improve the balance of the gut microbiota, promote the proliferation of beneficial bacteria, inhibit the growth of harmful bacteria, and enhance gut health and immune function.

[0062] Table 1. Statistical table of changes in various physical indicators in the experimental group and the control group (average values)

[0063]

[0064] in conclusion:

[0065] Through a 12-week experimental study, the results showed that the black sesame compound protein powder of this invention has significant effects on fat reduction and muscle gain, improving antioxidant capacity, regulating blood lipid levels, and improving intestinal microecology. Compared with existing technologies, this protein powder makes full use of the nutritional components of black sesame, combines high-quality protein sources and functional excipients, and achieves a balance between high nutritional value and multifunctionality through innovative preparation processes, demonstrating significant technological advantages and promising market application prospects.

[0066] It should be clarified that the present invention is not limited to the specific configurations and processes described above and shown in the figures. For the sake of brevity, detailed descriptions of known methods are omitted here. In the above embodiments, several specific steps are described and shown as examples. However, the method process of the present invention is not limited to the specific steps described and shown. Those skilled in the art can make various changes, modifications, and additions, or change the order of steps, after understanding the spirit of the present invention.

[0067] In this invention, features described and / or illustrated for one embodiment may be used in the same or similar manner in one or more other embodiments, and / or combined with or in place of features of other embodiments.

[0068] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, various modifications and variations of the embodiments of the present invention are possible. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A black sesame compound protein powder, characterized in that, The product comprises the following raw material components by weight: 5-15 parts black sesame extract microcapsules, 30-60 parts whey protein powder, 10-20 parts resistant dextrin, 1-5 parts probiotics, 0.05-0.15 parts natural sweetener, and 0.1-0.5 parts natural flavoring. The black sesame extract microcapsules are prepared using ultrasound-assisted ethanol extraction and sodium alginate-chitosan encapsulation technology; the probiotics are one or two of Lactobacillus acidophilus and Bifidobacterium.

2. The black sesame compound protein powder according to claim 1, characterized in that, The preparation method of the black sesame extract microcapsules includes the following steps: S1: After pretreatment, black sesame seeds are extracted for active ingredients using ultrasound-assisted ethanol extraction to obtain black sesame extract; the process parameters for ultrasound-assisted ethanol extraction are: ultrasound power 200~400W, extraction temperature 40~60℃, and extraction time 20~40 minutes. S2: Mix sodium alginate solution and black sesame extract evenly to form solution A, and use chitosan solution as solution B; the concentration of sodium alginate solution is 2wt.%~5wt.%, and the concentration of chitosan solution is 1wt.%~3wt.%. S3: Slowly add solution A to solution B, and carry out the reaction under heating and stirring conditions, so that sodium alginate and chitosan form microcapsule walls on the surface of the extract, encapsulating the extract; the reaction conditions are temperature 25~45℃, stirring speed 100~300rpm, and reaction time 20~60 minutes. S4: After filtration, washing, and drying, black sesame extract microcapsules are obtained.

3. The black sesame compound protein powder according to claim 1, characterized in that, The whey protein powder has a protein content of ≥80%, and the resistant dextrin has a dietary fiber content of ≥90%.

4. A method for preparing any one of the black sesame compound protein powders according to claims 1 to 3, characterized in that, Includes the following steps: S1: Weigh each raw material component according to the formula ratio; S2: Whey protein powder and a portion of resistant dextrin are premixed under low-speed shear to obtain a premix; the process parameters for the low-speed shear premix are: temperature 20~40℃, shear rate 100~500rpm, and mixing time 5~15 minutes. S3: Add the black sesame extract microcapsules and the remaining resistant dextrin to the premix and perform a high-speed shear mixing to obtain an intermediate mixture; the process parameters for the high-speed shear mixing are: temperature 25~45℃, shear rate 500~2000rpm, and mixing time 3~10 minutes. S4: Add probiotics, natural sweeteners, and flavorings to the intermediate mixture and perform a second high-speed shear mixing to obtain the finished black sesame compound protein powder; the process parameters for the second high-speed shear mixing are: temperature 30~50℃, shear rate 1000~3000rpm, and mixing time 8~15 minutes.

5. The preparation method according to claim 4, characterized in that, In step S2, the amount of the partial resistant dextrin used is 30% to 50% of the total resistant dextrin used; in step S3, the amount of the remaining resistant dextrin used is 50% to 70% of the total resistant dextrin used.

6. The application of any one of the black sesame compound protein powders according to claims 1 to 3 in the preparation of nutritional supplements, characterized in that, The nutritional supplement is used to improve human nutritional status, enhance immunity, provide antioxidant effects, regulate blood lipids, and improve intestinal function.

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