Novel ginseng for ulcerative colitis as well as preparation method and application thereof

Abstract

The invention discloses a preparation method and application of ginseng for ulcerative colitis. The preparation method comprises the following steps: S1, selecting complete ginseng and carrying out raw material pretreatment; s2, sieving loess, and mixing the sieved loess with water to form paste capable of wrapping the ginseng; s3, the ginseng is wrapped with loess, and the wrapping thickness is uniform; s4, putting the wrapped ginseng into a baking oven to be stewed at a constant temperature; and S5, taking out and cooling after simmering, removing loess on the surface, and airing or drying at low temperature until the processed product is stable. The obtained ginseng is applied to preparation of medicines for treating ulcerative colitis. Compared with the prior art, the novel ginseng for ulcerative colitis, the preparation method and the application have the advantages that the novel ginseng for ulcerative colitis is clear in technological parameters and stable in quality.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a novel ginseng for ulcerative colitis, its preparation method, and its application. Background Technology

[0002] Ginseng is the dried root and rhizome of the ginseng plant (Panax ginseng), a plant belonging to the Araliaceae family. It is a traditional and precious Chinese medicinal material.

[0003] Currently, the mainstream products on the market are mainly sun-dried ginseng and red ginseng, while research on simmered ginseng, especially ginseng wrapped in yellow mud, is relatively scarce.

[0004] In the theoretical system of traditional Chinese medicine, ulcerative colitis (UC) is characterized by diarrhea, abdominal pain, and bloody mucus stools, and is classified under the categories of "diarrhea" and "chronic dysentery".

[0005] The key to its pathogenesis lies in the deficiency of the root and the excess of the branch. The root deficiency is mainly spleen deficiency, while the branch excess is mostly caused by damp-heat and blood stasis. When the spleen is deficient, its function of transportation and transformation is impaired, and water and dampness flow down into the intestines, hence the persistent diarrhea. Prolonged illness damages the spleen yang, which can further affect the kidneys, forming a syndrome of deficiency of both the spleen and kidneys.

[0006] The "Chongtu San" emphasizes that ginseng wrapped in yellow mud can be used for abdominal distension, abdominal pain, and diarrhea caused by "weak spleen and kidney water". This is highly consistent with the pathogenesis of ulcerative colitis, which is based on spleen deficiency and downward flow of dampness. Furthermore, modern pharmacological studies have shown that ginseng contains active ingredients such as ginsenosides (e.g., Rb1, Rg1, Rg3), polysaccharides, and volatile oils, which have multiple effects, including regulating immune balance, inhibiting the release of inflammatory factors, repairing the intestinal mucosal barrier, and regulating intestinal flora. This provides a pharmacological basis for its treatment of ulcerative colitis. Ginseng wrapped in yellow mud may act more specifically on the core pathogenesis of "spleen deficiency and diarrhea" in ulcerative colitis through the specific components transformed after processing. However, the current practice of ginseng wrapped in yellow mud relies heavily on experience, lacking standardized parameters for temperature, time, and the amount of auxiliary materials, resulting in unstable quality of the processed products and limited clinical application. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to overcome the above-mentioned technical defects and provide a novel ginseng for ulcerative colitis with clear process parameters and stable quality, a preparation method thereof, and its application.

[0008] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a method for preparing ginseng for ulcerative colitis, comprising the following steps: S1: Select whole ginseng roots for raw material pretreatment; S2: Take loess, sift it, mix it with water to form a mud paste that can wrap ginseng; S3: Ginseng is wrapped in loess, with a uniform coating thickness; S4: Place the wrapped ginseng in an oven and simmer at a constant temperature; S5: After simmering, remove and cool, remove the surface yellow soil, and air dry or dry at low temperature until the product is stable.

[0009] Preferably, step S1 further includes removing mud and sand and fibrous roots from the whole ginseng and drying it until the surface is dry.

[0010] Preferably, in step S2, the loess is passed through a No. 4 sieve, and the loess and water are mixed at a mass ratio of 2:1.

[0011] Preferably, in step S3, human ginseng is wrapped in loess at a mass ratio of 1:0.8.

[0012] Preferably, the simmering temperature in step S4 is 78℃~82℃, and the simmering time is 17.5h~18.5h.

[0013] Another aspect of the present invention discloses a ginseng prepared by the above method for use in ulcerative colitis.

[0014] Another aspect of this invention discloses the use of ginseng in the preparation of a medicament for treating ulcerative colitis.

[0015] Preferably, the dosage of the ginseng is 1.17 g / kg to 4.68 g / kg.

[0016] Preferred methods include reducing the levels of pro-inflammatory factors TNF-α and IL-6, increasing the level of anti-inflammatory factor IL-10, reducing myeloperoxidase activity, repairing intestinal barrier function, and significantly upregulating the expression of intestinal tight junction proteins Occludin and ZO-1.

[0017] The advantages of this invention compared to the prior art are: Process standardization: The Box-Behnken response surface methodology was applied to examine the content and appearance of ginsenosides Rg1, Re, Rb1, total saponins, total polysaccharides, and ginseng. The three factors of simmering time, simmering temperature, and amount of loess were examined. The analytic hierarchy process and the entropy weight method were combined to obtain a comprehensive score as the response value, and the process of simmering ginseng wrapped in loess was optimized. The pharmacodynamic results are significant, the application prospects are clear, the efficacy is comparable to that of positive control drugs, and the mechanism of action has been explored in depth. Great application potential: It provides data support for the clinical application and product development of ginseng wrapped in yellow mud, and promotes the modernization of traditional processing methods. Attached Figure Description

[0018] Figure 1 This is a diagram illustrating weight changes and DAI scores.

[0019] Figure 2 This is a schematic diagram of a comparative analysis of colon length.

[0020] Figure 3 This is a schematic diagram of inflammatory factors and MPO levels.

[0021] Figure 4 These are HE-stained pathological images of colon tissue from each group of mice.

[0022] Figure 5 These are immunohistochemical staining images of colon tissue from each group of mice.

[0023] As shown in the figure: Figure 1 * Compared with the blank group P <0.05,** P <0.01, *** p <0.001; compared with the model group, + P <0.05, ++ P <0.01,+++ P <0.001; inter-group comparisons, # P <0.05, ## P <0.01, ### p <0.001; Blank control group (C), model group (M), positive drug group (MES), sun-dried ginseng group (SDG), high-dose ginseng wrapped in yellow mud group (YMG-H), medium-dose group (YMG-M), low-dose group (YMG-L); Figure 2 In comparison with the blank control group, * P<0.05, ** P<0.01, *** p<0.001; compared with the model group, + P<0.05, ++ P<0.01, +++ P<0.001; among the drug administration groups, # P<0.05, ## P<0.01, ### p<0.001; blank control group (C), model group (M), positive control group (MES), sun-dried ginseng group (SDG), high-dose ginseng wrapped in yellow mud group (YMG-H), medium-dose group (YMG-M), and low-dose group (YMG-L). Figure 3 In comparison with the blank control group, * P<0.05, ** P<0.01, *** p<0.001; compared with the model group, + P<0.05, ++ P<0.01, +++ P<0.001; among the drug administration groups, # P<0.05, ## P<0.01, ### p<0.001; blank control group (C), model group (M), positive control group (MES), sun-dried ginseng group (SDG), high-dose ginseng wrapped in yellow mud group (YMG-H), medium-dose group (YMG-M), and low-dose group (YMG-L). Figure 4 The groups are: blank control group (A), model group (B), positive drug group (C), sun-dried ginseng group (D), high-dose ginseng wrapped in yellow mud (E), medium-dose group (F), and low-dose group (G). Figure 5 In the study: blank control group (A, a), model group (B, b), positive control group (C, c), sun-dried ginseng group (D, d), high-dose ginseng wrapped in yellow mud group (E, e), medium-dose group (F, f), and low-dose group (G, g); (AB) are the immunohistochemical results of Occludin, a tight junction protein in mouse colon, and (ab) are the immunohistochemical results of ZO-1, a tight junction protein in mouse colon. Detailed Implementation

[0024] The present invention will now be described in further detail with reference to the accompanying drawings.

[0025] Processing technique of ginseng wrapped in yellow mud 1.1: Raw material pretreatment: Select whole ginseng, remove mud and sand and fibrous roots, and dry until the surface is dry.

[0026] 1.2: Mixing mud: Take loess and pass it through a No. 4 sieve. Mix the loess and water at a ratio of about 2:1 (by mass) to form a mud paste that can wrap ginseng.

[0027] 1.3: Wrapping: Wrap the ginseng with loess in a ratio of 1:0.8 (by weight), that is, use approximately 80g of loess paste to completely wrap every 100g of ginseng, ensuring a uniform coating thickness. For ginseng of different sizes, use the weight ratio to control the amount of loess to ensure consistent coating thickness.

[0028] 1.4: Stewing: Place the wrapped ginseng in an oven and stew at 80℃ for 18 hours (experimental optimal value); ensure the temperature remains constant to avoid localized high temperatures that could cause scorching or cracking. The laboratory response surface prediction was approximately 78.24℃ / 17.77 h, which was corrected and verified through actual operation to be 80℃ / 18h, with a loess ratio of 1:0.8. The overall process verification score was close to the predicted value.

[0029] 1.5: Removal, Cooling, and Descaling: After simmering, remove the product and allow it to cool. Remove the surface yellow soil (traditionally, this can be done by washing or brushing). Air dry or low-temperature dry until the product is stable (moisture content should be within the target range). Record the descaling method to control total ash residue.

[0030] Pharmacodynamic studies: After 3 days of acclimatization, the mice were randomly divided into 7 groups (n=8): blank control group (Control), model group (Model), positive control group (MES, 500mg / kg), sun-dried ginseng group (SDG, 4.68g / kg), low-dose ginseng wrapped in yellow mud group (YMG-L, 1.17g / kg), medium-dose ginseng wrapped in yellow mud group (YMG-M, 2.34g / kg), and high-dose ginseng wrapped in yellow mud group (YMG-H, 4.68g / kg).

[0031] Modeling and drug administration: After grouping, the control group received free access to drinking water daily for 10 days, while all other groups received free access to 3.5% DSS daily for 7 days, followed by free access to drinking water for 3 consecutive days starting on day 8. The control and model groups were administered 0.2 ml of physiological saline daily by gavage, while the treatment groups were administered 0.2 ml of the drug daily by gavage, all for 10 days. On day 11, the mice were sacrificed, and their blood, colon tissue, spleen, liver, kidneys, and intestinal contents were collected and stored at −80 °C.

[0032] Weight changes and DAI score: Compared with the control group, the model group mice showed a significant decrease in body weight of 30.72%, and their DAI score increased from 0 to 8.6, indicating successful UC modeling. The high-dose ginseng wrapped in yellow mud and baked showed an 8.9% increase in body weight compared to the model group, indicating that it significantly slowed down weight loss, with effects similar to the positive control group. However, the SDG group showed a 0.4% decrease in body weight compared to the model group, indicating that raw ginseng did not slow down weight loss. This clearly suggests that the yellow mud-wrapping and baking process enhances the efficacy of ginseng in treating ulcerative colitis. (See...) Figure 1 ) Colon length: Comparative analysis showed that the (YMG-H) group and the SDG group increased by 26.3% and 21.26% respectively compared with the model group. The high-dose ginseng wrapped in yellow mud (YMG-H) group had a significantly stronger reversal effect on colon shortening than the sun-dried ginseng group (SDG). The colon length in the YMG-H group was close to the normal level, while the colon length in the SDG group was still significantly shorter than that in the blank control group, indicating that the processed ginseng wrapped in yellow mud has a better effect in inhibiting intestinal tissue pathological damage. (See...) Figure 2 ) Inflammatory factors and MPO levels: In regulating inflammatory responses, ginseng wrapped in yellow mud exhibited a more comprehensive advantage than sun-dried ginseng. Compared with the sun-dried ginseng group (SDG), the high-dose ginseng wrapped in yellow mud (YMG-H) group, compared with the model group, showed a 11.8% and 11.7% reduction in pro-inflammatory factors (TNF-α, IL-6), respectively, and a 5.9% reduction in myeloperoxidase (MPO) activity.

[0033] Simultaneously, it significantly increased the level of the anti-inflammatory factor (IL-10) by 9.4% compared to the model group. This indicates that the processing technique not only enhanced its anti-inflammatory effect but also improved its antioxidant stress resistance. (See...) Figure 3 ) Colon histopathology and immunohistochemistry: The model group showed disordered colonic structure and inflammatory cell infiltration; the high-dose ginseng-wrapped-in-yellow mud group largely restored colonic structure, reduced inflammatory cells, and increased goblet cells and crypt structures. The model group exhibited disordered expression of tight junction proteins (Occludin and ZO-1); the high-dose ginseng-wrapped-in-yellow mud group significantly restored protein expression and enhanced intestinal barrier function. (See...) Figure 4 , 5 ).

[0034] This invention, through rigorous parallel controlled experiments, demonstrates that, at the same dosage, the ginseng wrapped in yellow mud exhibits significantly superior overall efficacy in treating ulcerative colitis compared to unprocessed sun-dried ginseng. Particularly in key pathological processes, such as inhibiting intestinal inflammation, repairing colonic tissue damage, and upregulating tight junction protein expression, the ginseng wrapped in yellow mud demonstrates potent effects not found in sun-dried ginseng, constituting the core competitive advantage of this invention.

[0035] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0036] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0037] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A method for preparing ginseng for ulcerative colitis, characterized in that: Includes the following steps: S1: Select whole ginseng roots for raw material pretreatment; S2: Take loess, sift it, mix it with water to form a mud paste that can wrap ginseng; S3: Ginseng is wrapped in loess, with a uniform coating thickness; S4: Place the wrapped ginseng in an oven and simmer at a constant temperature; S5: After simmering, remove and cool, remove the surface yellow soil, and air dry or dry at low temperature until the product is stable.

2. The method for preparing ginseng for ulcerative colitis according to claim 1, characterized in that: S1 also includes removing mud and sand and fibrous roots from whole ginseng and drying it until the surface is dry.

3. The method for preparing ginseng for ulcerative colitis according to claim 1, characterized in that: In S2, the loess is passed through a No. 4 sieve, and the loess and water are mixed at a mass ratio of 2:

1.

4. The method for preparing ginseng for ulcerative colitis according to claim 1, characterized in that: The S3 contains human ginseng wrapped in loess at a mass ratio of 1:0.

8.

5. The method for preparing ginseng for ulcerative colitis according to claim 1, characterized in that: The simmering temperature in S4 is 78℃~82℃, and the simmering time is 17.5h~18.5h.

6. A ginseng used for ulcerative colitis, characterized in that: It is prepared by any one of the preparation methods described in claims 1-5.

7. The use of ginseng for ulcerative colitis as described in claim 6 in the preparation of a medicament for treating ulcerative colitis.

8. The application according to claim 7, characterized in that: The dosage of the ginseng is 1.17 g / kg to 4.68 g / kg.

9. The application according to claim 7, characterized in that: This includes reducing the levels of pro-inflammatory factors TNF-α and IL-6, increasing the level of anti-inflammatory factor IL-10, reducing myeloperoxidase activity, repairing intestinal barrier function, and significantly upregulating the expression of intestinal tight junction proteins Occludin and ZO-1.

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