Nano-selenium foliar fertilizer capable of rapidly increasing selenium content of wheat as well as preparation method and application of nano-selenium foliar fertilizer

Abstract

The invention discloses a nano-selenium foliar fertilizer capable of rapidly increasing the selenium content of wheat and a preparation method of the nano-selenium foliar fertilizer, and belongs to the technical field of agricultural planting. The nano-selenium leaf fertilizer consists of the following components: 0.3-0.8 mM of nano-selenium, 0.1%-1% (m / v) of attapulgite irradiated by electron beams, 0.35-1.4% (m / v) of bacillus metabolite and the balance of water. The method has the beneficial effects that compared with the prior art and the traditional inorganic selenium fertilizer, the toxicity of nano-selenium is lower, and the damage to plants is smaller. In the use process, even under the condition of relatively high concentration, phytotoxicity to plants is not easy to generate; meanwhile, the nano-selenium is extremely small in particle size, is at a nano level and has a relatively large specific surface area, so that the nano-selenium can more easily penetrate through cuticles and cell walls on the surfaces of wheat leaves, and can be quickly absorbed and utilized by the wheat, the efficiency of supplementing the selenium element for the wheat is higher, and the selenium-rich wheat fertilizer is more environment-friendly.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and in particular to a nano-selenium foliar fertilizer that rapidly increases the selenium content of wheat, its preparation method, and its application. Background Technology

[0002] Currently, the selenium content in soils in most natural environments is at a low level, making it difficult for ordinary wheat to meet the normal selenium requirements of the human body. Traditional methods for increasing the selenium content of wheat have many limitations. For example, some methods of applying selenium to soil have low utilization rates and are easily affected by factors such as soil physicochemical properties, microbial activity, and rainfall. This leads to unstable selenium fixation, loss, or transformation efficiency in the soil, making it difficult to accurately control the amount of selenium absorbed by wheat. Furthermore, excessive selenium application may pollute the soil environment, disrupt the soil ecological balance, and affect the quality of surrounding water bodies.

[0003] Furthermore, while existing foliar spraying techniques for selenium fertilizer can quickly replenish selenium to a certain extent, they often require multiple applications, and controlling the spraying time and concentration is quite complex. If the spraying concentration is too high, it may scorch wheat leaves, affecting photosynthesis and normal growth and development; if the concentration is too low or the spraying timing is inappropriate, the expected selenium enrichment effect cannot be achieved. At the same time, simply increasing selenium content is insufficient to quickly and efficiently raise wheat selenium content to the ideal selenium-enriched standard range, and may be accompanied by adverse consequences such as decreased wheat yield or deterioration in quality. This significantly limits the large-scale production and application of selenium-enriched wheat. Therefore, there is an urgent need for an innovative method that can quickly, stably, efficiently, and environmentally friendly increase the selenium content of wheat.

[0004] Chinese patent application document CN115477554A discloses a selenium-enriched foliar fertilizer and its preparation method and application. The preparation method includes the following steps: S1. Fermentation stage one: S1.1 Molasses, urea, sodium chloride, dipotassium hydrogen phosphate, and water are added to fermentation tank 1, stirred evenly, heated and sterilized, and then cooled; S1.2 Seed liquid is obtained by culturing inoculated bacteria in fermentation medium, and added to fermentation tank 1 at an inoculation rate of 1%, and water is added for fermentation; S2. Fermentation stage two: Molasses, sodium selenite, ammonium molybdate, boric acid, marine fish enzymatic hydrolysis concentrate, selenoamino acids, plant growth promoters, and water are added to fermentation tank 2, stirred evenly, heated and sterilized, and then cooled to obtain fermentation feed; S3. Fermentation stage three: Fermentation feed is added to fermentation tank 1 of fermentation stage one and mixed for fermentation to obtain selenium-enriched foliar fertilizer. This patented product uses high-density microbial fermentation technology to produce selenium-enriched foliar fertilizer, which has the effects of stress resistance and disease prevention, increased yield and quality, and efficient selenium enrichment in plants. It can also increase selenium levels in the human body, enhancing physical and mental health. However, the preparation process is cumbersome, and the fertilizer's effectiveness is relatively poor; therefore, further research and improvement are needed. Summary of the Invention

[0005] The technical problem to be solved by this invention is how to provide a method for rapidly increasing the selenium content of wheat.

[0006] The present invention solves the above-mentioned technical problems through the following technical means: The first aspect of this invention proposes a nano-selenium foliar fertilizer for increasing the selenium content of wheat, which is composed of the following components: 0.3~0.8 mM nano-selenium, 1‰~1% (m / v) attapulgite soil irradiated by electron beam, 0.35-1.4% (m / v) Bacillus metabolites, and the balance being water; The irradiated nano-attapulgite is specifically prepared by irradiating the nano-attapulgite with a high-energy electron beam accelerator with a dose of 10~400 kGy for 1~24h.

[0007] Preferably, the concentration of the nano-selenium is 0.4~0.6mM.

[0008] Preferably, the concentration of the nano-selenium is 0.4 mM.

[0009] Preferably, the particle size of the nano-selenium is 30~60nm.

[0010] Preferably, the concentration of the attapulgite soil after electron beam irradiation is 0.5% (m / v).

[0011] Preferably, the attapulgite soil is irradiated with a high-energy electron beam accelerator with a dose of 40 kGy for 20 h.

[0012] Preferably, the attapulgite soil is irradiated for 24 hours using a high-energy electron beam accelerator with a dose of 10 kGy.

[0013] A second aspect of the present invention provides a method for preparing the above-mentioned nano-selenium foliar fertilizer, comprising the following steps: The nano-selenium, electron beam irradiated attapulgite, Bacillus metabolites, and water are mixed evenly to achieve a final concentration of 0.3-0.8 mM for the nano-selenium, 1‰-1% (m / v) for the electron beam irradiated attapulgite, and 0.35-1.4% (m / v) for the Bacillus metabolites.

[0014] A third aspect of the present invention proposes the application of the above-mentioned nano-selenium foliar fertilizer in increasing the selenium content of wheat.

[0015] The fourth aspect of the present invention provides a method for increasing the selenium content of wheat, comprising the following steps: spraying the above-mentioned nano-selenium foliar fertilizer on wheat in the early flowering stage in the field.

[0016] Preferably, the application rate is 8-12 L / mu, and more preferably 10 L / mu.

[0017] The beneficial effects of this invention are as follows: 1. Compared to existing technologies and traditional inorganic selenium fertilizers, nano-selenium exhibits lower toxicity and less damage to plants. Even at higher concentrations, it is less likely to cause phytotoxicity to plants. Furthermore, the extremely small particle size of nano-selenium, at the nanoscale, provides a large specific surface area, allowing it to more easily penetrate the cuticle and cell walls of wheat leaves, thus enabling rapid absorption and utilization by wheat. This results in higher efficiency in supplementing wheat with selenium and is more environmentally friendly.

[0018] 2. When the percentage of each raw material is below the above range, there is no significant control effect. When the percentage of each raw material exceeds the above range, the control effect will not increase, but the control cost will increase. Furthermore, excessively high concentrations will cause yellowing of leaves and affect the normal growth of wheat. Attached Figure Description

[0019] Figure 1 This is a comparison chart showing the selenium content of wheat grains harvested after the flowering stage in Example 1 and Comparative Example 1 of the present invention, where nano-selenium fertilizer was used to treat wheat in the field. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise defined, the technical terms used below have the same meaning as understood by those skilled in the art.

[0021] Unless otherwise specified, the test materials and reagents used in the following examples are commercially available or prepared by known methods.

[0022] Unless otherwise specified, all techniques or conditions described in the embodiments can be performed in accordance with the techniques or conditions described in the literature in this field or in the product manual. Unless otherwise specified, the quantitative experiments in the following embodiments are all repeated three times or more, and the results are averaged.

[0023] Bacillus metabolites are obtained through the following process: [The process involves] processing Bacillus... Bacillus.sp HZ3 (CCTCC NO: M20221472) was placed in a culture medium and fermented (28℃, 2.5d). The OD of the bacterial culture was measured... 600 When the temperature reaches 1.5°C, collect the fermentation broth and treat it with ultrasound at 25 kHz for 2 minutes to obtain the final product.

[0024] Example 1: A nano-selenium foliar fertilizer for increasing the selenium content of wheat is composed of the following components: 0.4 mM nano-selenium (particle size 30~60nm), 0.5% (m / v) electron beam irradiated attapulgite, 1.18% (m / v) Bacillus metabolites, and the balance being water.

[0025] The preparation method of the above-mentioned nano-selenium foliar fertilizer includes the following steps: (1) Attapulgite was irradiated with a high-energy electron beam accelerator with a dose of 40 kGy for 20 h to obtain irradiated attapulgite. (2) The electron beam irradiated attapulgite obtained above is mixed with nano-selenium, Bacillus metabolites and water in proportion to make the final concentration of nano-selenium 0.4 mM, the final concentration of electron beam irradiated attapulgite 0.5% (m / v) and the final concentration of Bacillus metabolites 1.18% (m / v) to obtain the final product.

[0026] A method for increasing the selenium content of wheat includes the following steps: spraying the nano-selenium foliar fertilizer prepared in this embodiment at the early flowering stage of wheat (Zhenmai 12 (Xibu Town) and Yangfumai 13 (Shiyang Town)) in the field at a dosage of 10 L / mu.

[0027] Shiyang Town, He County, Ma'anshan City, Anhui Province (31°56′24″N, 118°16′54″E) Xibu Town, He County, Ma'anshan City, Anhui Province (31°46′17″N, 118°17′08″E).

[0028] After spraying with nano-selenium foliar fertilizer, the selenium content in whole wheat flour processed from harvested wheat grains significantly increased to 0.1 mg / kg. Figure 1 As shown, increased selenium content can enhance the nutritional value of products. This helps improve the economic benefits for farmers and food processing enterprises.

[0029] Example 2: A nano-selenium foliar fertilizer for increasing the selenium content of wheat is composed of the following components: 0.3 mM nano-selenium (particle size 30~60nm), 1‰ (m / v) electron beam irradiated attapulgite soil, 0.35% (m / v) Bacillus metabolites, and the balance being water.

[0030] The preparation method of the above-mentioned nano-selenium foliar fertilizer includes the following steps: (1) Attapulgite was irradiated with a high-energy electron beam accelerator with a dose of 400 kGy for 1 h to obtain irradiated attapulgite. (2) The electron beam irradiated attapulgite prepared above is mixed with nano-selenium and water in a certain proportion to make the final concentration of nano-selenium 0.3 mM, the final concentration of electron beam irradiated attapulgite 1‰ (m / v), and the final concentration of Bacillus metabolites 0.35% (m / v) to obtain the final product.

[0031] A method for increasing the selenium content of wheat includes the following steps: spraying the nano-selenium foliar fertilizer prepared in this embodiment at the early flowering stage of wheat in the field, with a dosage of 8 L / mu.

[0032] Example 3: A nano-selenium foliar fertilizer for increasing the selenium content of wheat is composed of the following components: 0.8 mM nano-selenium (particle size 30~60nm), 1% (m / v) electron beam irradiated attapulgite, 1.4% (m / v) Bacillus metabolites, and the balance being water.

[0033] The preparation method of the above-mentioned nano-selenium foliar fertilizer includes the following steps: (1) Attapulgite was irradiated with a high-energy electron beam accelerator with a dose of 10 kGy for 24 h to obtain irradiated attapulgite. (2) The electron beam irradiated attapulgite prepared above is mixed with nano-selenium and water in a certain proportion to make the final concentration of nano-selenium 0.8mM, the final concentration of electron beam irradiated attapulgite 1% (m / v), and the final concentration of Bacillus metabolites 1.4% (m / v) to obtain the final product.

[0034] A method for increasing the selenium content of wheat includes the following steps: spraying the nano-selenium foliar fertilizer prepared in this embodiment at the early flowering stage of wheat in the field, with a dosage of 12 L / mu.

[0035] Comparative Example 1: The difference between this comparative example and Example 1 is that no fertilizer solution was sprayed; instead, an equal amount of distilled water was sprayed.

[0036] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nano-selenium foliar fertilizer for increasing the selenium content of wheat, characterized in that, It consists of the following components: 0.3~0.8 mM nano selenium, 1‰~1% (m / v) electron beam irradiated attapulgite, 0.35-1.4% (m / v) Bacillus metabolites, and the balance being water; The irradiated nano-attapulgite is specifically prepared by irradiating the nano-attapulgite with a high-energy electron beam accelerator with a dose of 10~400 kGy for 1~24h.

2. The nano-selenium foliar fertilizer according to claim 1, characterized in that, The nano-selenium has a particle size of 30~60nm.

3. The nano-selenium foliar fertilizer according to claim 1, characterized in that, The concentration of the nano-selenium is 0.4~0.6mM.

4. The nano-selenium foliar fertilizer according to claim 1, characterized in that, The concentration of the nano-selenium is 0.4 mM.

5. The nano-selenium foliar fertilizer according to claim 1, characterized in that, The concentration of the attapulgite soil after electron beam irradiation is 0.5% (m / v).

6. The nano-selenium foliar fertilizer according to claim 1, characterized in that, Attapulgite was irradiated with a high-energy electron beam accelerator with a dose of 40 kGy for 20 h.

7. The method for preparing the nano-selenium foliar fertilizer according to any one of claims 1-6, characterized in that, Includes the following steps: The nano-selenium, electron beam irradiated attapulgite, Bacillus metabolites, and water are mixed evenly to achieve a final concentration of 0.3-0.8 mM for the nano-selenium, 1‰-1% (m / v) for the electron beam irradiated attapulgite, and 0.35-1.4% (m / v) for the Bacillus metabolites.

8. The application of the nano-selenium foliar fertilizer according to any one of claims 1-6 in increasing the selenium content of wheat.

9. A method for increasing the selenium content of wheat, characterized in that, Includes the following steps: The nano-selenium foliar fertilizer described in any one of claims 1-6 can be applied to wheat foliar fertilizer during the early flowering stage in the field.

10. The method according to claim 9, characterized in that, The application rate is 8-12 L / mu.

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