Composition with skin repairing effect as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition with a skin repairing effect, the composition comprises the following components in parts by mass: 5-20 parts of chamomile extract, 8-15 parts of adiantum extract, 5-10 parts of cattail extract and 5-15 parts of selfheal extract, and the invention also discloses a preparation method and application of the composition. The composition disclosed by the invention is safe and stable, can play a good skin repairing effect, and has a wide market prospect.

Description

Technical Field

[0001] This invention relates to the field of composition technology, and more specifically to a composition with skin repair effects and its preparation method. Background Technology

[0002] In the cosmetics industry, traditional Chinese medicine compound formulas derived from natural plants are highly favored due to their gentle effects and synergistic effects targeting multiple targets. Currently, developing compound traditional Chinese medicine products with multiple functions has become an important trend. This invention relates to a composition consisting of chamomile, maidenhair fern, calamus, and prunella vulgaris. Although these four plant components have been proven to have unique activities in both traditional medicine and modern research, existing technologies are mostly limited to their single application or simple combination, failing to fully utilize their inherent synergistic potential, resulting in products with limited functions and application scope.

[0003] Specifically, the current understanding and limitations of each ingredient are as follows: Chamomile is known to have anti-inflammatory, antibacterial, and soothing effects, but current technologies mostly use it alone in calming or anti-allergy products. Its potential to synergistically enhance whitening or anti-inflammatory effects with other ingredients has not yet been explored. Maidenhair fern is traditionally used for clearing heat and detoxifying, promoting diuresis and reducing swelling, and has some efficacy in treating mastitis. However, current research has not focused enough on it, its active ingredients are not studied in depth, and it often plays a secondary role in compound preparations. Its powerful potential for reducing swelling and dissipating nodules has not been effectively utilized. Research on calamus has mainly focused on the sedative and nodule-enhancing effects of its volatile oil. Recent studies have found that its polysaccharide components have significant inhibitory activity against tyrosinase, showing potential for whitening and fading spots. However, this characteristic has not been emphasized in current technologies, and there is a lack of research on enhancing its whitening effect through formulation. Prunella vulgaris, as a traditional heat-clearing and fire-purging herb, excels at clearing liver fire and dissipating stagnation, and has clear anti-inflammatory and immunomodulatory effects. Current applications are mostly limited to treating red, swollen, and painful eyes or scrofula, and the systematic research on its core "nodule-dispersing" effect and its compatibility with other anti-inflammatory plant components is still insufficient.

[0004] In summary, existing technologies have significant shortcomings: First, the related products have limited functions and fail to integrate the diverse activities of each ingredient to achieve compound effects; second, there is a lack of systematic research on the synergistic effects between the ingredients, especially regarding how to organically combine the whitening potential of Acorus calamus, the heat-clearing, detoxifying, and nodule-dispersing effects of Adiantum capillus-veneris and Prunella vulgaris, and the soothing and anti-inflammatory effects of chamomile; finally, the application areas are narrow, and a comprehensive solution integrating "whitening and spot-fading, anti-inflammatory and swelling-reducing, heat-clearing and detoxifying, and calming and soothing" has not been developed to meet modern needs.

[0005] Therefore, there is an urgent need in this field for a new composition that can integrate the advantages of the above four plants through scientific formulation, overcome the shortcomings of existing technologies, and achieve synergistic effects among the components to meet the market demand for efficient and multifunctional natural products. Summary of the Invention

[0006] One of the purposes of this application is to provide a composition with skin repair effects, which is made by compounding chamomile extract, maidenhair fern extract, calamus extract and prunella vulgaris extract, and is safe, stable and has good skin repair effects.

[0007] Another objective of this application is to provide a method for preparing a composition with skin repair effects, which employs supercritical extraction and low-temperature microfluidic processes to prepare a composition with good skin repair effects.

[0008] In addition, this application also provides the application of the composition with skin repair function, that is, the application of the composition with skin repair function of this application to the preparation of skin care products.

[0009] To achieve the above objectives, this application provides a composition with skin repair effects, the composition comprising chamomile extract, maidenhair fern extract, calamus extract and prunella vulgaris extract;

[0010] Furthermore, the mass fractions of each component in the composition are 5-20 parts of chamomile extract, 8-15 parts of maidenhair fern extract, 5-10 parts of calamus extract, and 5-15 parts of prunella vulgaris extract.

[0011] The second technical solution provided by this invention is: a method for preparing the above-mentioned composition, comprising the following steps:

[0012] Step 1: Chamomile, maidenhair fern, and calamus root are pulverized and extracted separately by reflux with ethanol-water solution for 1 hour, 3 times. The extracts are then filtered through filter cloth and filter membrane (0.45μm) successively. The filtrate is collected and then distilled under reduced pressure using a rotary evaporator to remove the ethanol. The solid material is then freeze-dried at low temperature to prepare chamomile extract, maidenhair fern extract, and calamus root extract.

[0013] Step 2: After crushing the Prunella vulgaris herb, put it into a supercritical CO2 extraction vessel, collect the filtrate after extraction, and use a rotary evaporator to perform vacuum distillation to remove ethanol. Then, freeze-dry the solid material at low temperature to prepare Prunella vulgaris extract.

[0014] Step 3: Mix chamomile extract, maidenhair fern extract, calamus extract and prunella vulgaris extract, then add water and 1,3-butanediol and mix. Process using a low-temperature microfluidic device. The working temperature of the low-temperature microfluidic treatment is 10℃ and the working pressure is 15000psi. The treatment is repeated three times to prepare a traditional Chinese medicine composition with skin repair effects. The particle size of the traditional Chinese medicine composition is less than 100nm.

[0015] Furthermore, the supercritical extraction pressure in step two is 20 MPa-40 MPa.

[0016] Furthermore, the temperature of supercritical extraction in step two is 45℃-55℃.

[0017] Furthermore, the separation pressure of supercritical extraction in step two is 5MPa-10MPa.

[0018] Furthermore, the separation temperature of the supercritical extraction in step two is 30℃-60℃.

[0019] Furthermore, the operating temperature of the microfluidic treatment in step three is 0℃-70℃.

[0020] Furthermore, in step three, the working pressure of the microjets is 1000psi-30000psi.

[0021] The third technical solution provided by the present invention is: the application of the above-mentioned composition with skin repair function in the preparation of cosmetics.

[0022] Furthermore, the cosmetic is a topical skin preparation made by using the aforementioned skin-repairing composition as the active ingredient, plus conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

[0023] Furthermore, the cosmetics include one or more of the following: aqueous solutions, creams, cleansers, serums, masks, and mud masks.

[0024] Furthermore, the amount of the skin-repairing composition added to the cosmetic is 0.1% - 10%.

[0025] The composition disclosed in this application strictly adheres to the "principal, assistant, adjuvant, and guide" principle of traditional Chinese medicine: Chamomile, the principal herb, is slightly cold in nature and enters the liver and lung meridians, primarily clearing heat and wind, calming the liver and improving eyesight. Its flavonoid components inhibit histamine release, laying the foundation for anti-allergy and repair. The assistant herbs, maidenhair fern and calamus, work synergistically. Maidenhair fern clears heat and detoxifies, while calamus resolves phlegm and unblocks the meridians. Together, they regulate inflammatory pathways such as NF-κB, significantly inhibiting inflammatory factors such as TNF-α and IL-6, enhancing anti-inflammatory and swelling-reducing effects. The adjuvant herb, prunella vulgaris, clears the liver and disperses nodules. Its iridoid components enhance anti-inflammatory activity, and it acts as a natural permeation enhancer to improve transdermal absorption. Furthermore, its pungent and dispersing properties harmonize the overall cold nature of the formula, preventing any imbalance in the medicinal properties. The combination of these herbs, with their orderly principal and assistant roles and synergistic effects, collectively achieves the effects of clearing heat and wind, detoxifying and anti-allergic, reducing swelling and relieving itching.

[0026] On the other hand, the composition described in this application has been confirmed by network pharmacology analysis to have a multi-target mechanism of action. Based on the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP, https: / / old.tcmsp-e.com / tcmsp.php), a systematic search was conducted on the chemical components of chamomile, maidenhair fern, calamus, and prunella vulgaris in the composition, and their targets related to skin sensitivity were screened.

[0027] Analysis results indicate that the composition targets a total of 192 common sites related to skin sensitivity issues (see...). Figure 1 Through protein interaction network construction and topological analysis, the core targets among the common targets were found to include TNF, AKT1, STAT3, EGFR, CASP3, BCL2, NFKB1, SRC, ESR1, and JUN (see [link to relevant documentation]). Figure 2 The active ingredients of this composition can simultaneously act on the aforementioned key targets, forming a network-based regulatory effect. The intersection results of the compositions were analyzed using the DAVID database for KEGG analysis of potential targets for skin sensitivity and inflammation (see [link to analysis]). Figure 3 (P < 0.05 was selected). The study showed that multiple inflammatory mechanisms collectively lead to skin barrier dysfunction and pathological hypersensitivity. The heat-clearing and detoxifying components of Prunella vulgaris and Adiantum capillus-veneris synergistically inhibit the core inflammatory pathway TNF and its downstream NFKB1 pathway, blocking the inflammatory cascade response at its source. The effective components of Acorus gramineus and chamomile balance cell proliferation and differentiation by regulating the EGFR-SRC-STAT3 signaling axis. Simultaneously, the active components of chamomile inhibit abnormal apoptosis and promote cell survival by regulating the AKT1-BCL2-CASP3 signaling module. Furthermore, the entire formula works synergistically on stress transcription factors such as JUN, comprehensively regulating gene expression profiles. This multi-target intervention model explains the synergistic pharmacological mechanism of this compound in anti-inflammatory, anti-allergic, soothing, sedative, and tissue repair effects at the molecular level.

[0028] Compared with the prior art, this application has the following beneficial effects:

[0029] This application provides a composition with skin repair effects. By rationally and scientifically combining chamomile, maidenhair fern, calamus, and prunella vulgaris, and incorporating the principles of traditional Chinese medicine's "principal, assistant, adjuvant, and guide" formula, a synergistic effect is achieved. Furthermore, based on network pharmacology analysis, the specific target mechanism is clarified. The skin repair composition provided by this application exhibits excellent anti-inflammatory, anti-allergic, and antioxidant activities, is gentle and non-irritating, and can comprehensively soothe and repair the skin. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0031] Figure 1 This is a target diagram illustrating the combined effects of the traditional Chinese medicine composition of the present invention and sensitive skin problems.

[0032] Figure 2 This is a protein-protein interaction network analysis diagram of the overlapping target sites of the traditional Chinese medicine composition of the present invention and sensitive skin;

[0033] Figure 3 This is an enrichment map of the KEGG pathway, the core target of the traditional Chinese medicine composition of the present invention. Detailed Implementation

[0034] The technical solutions of this application will be described in detail below with reference to preferred embodiments to facilitate understanding of the essence and advantages of this application. Although these embodiments provide a specific implementation background for understanding this application, this application can be implemented in many different forms and is not limited to the specific details described below. The true scope of this application is defined by the appended claims and their equivalents.

[0035] The substrates and reagents involved in the examples are commercially available or can be prepared by those skilled in the art using known methods.

[0036] Preparation method example

[0037] Preparation of Chamomile Extract-1:

[0038] Chamomile herb was pulverized and extracted by reflux with an ethanol-water solution (70 wt% ethanol content) at a material-to-liquid ratio of 1:15, for 2 hours, and twice. The extract was filtered through a filter cloth and a 0.45 μm filter membrane. The filtrate was collected and then eluted with AB-8 resin with 70% ethanol as the eluent for 3 BV. The eluent was then subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare chamomile extract-1, wherein the total flavonoid content of chamomile extract-1 is ≥10%.

[0039] Preparation of Chamomile Extract-2:

[0040] Chamomile herb was pulverized and extracted by reflux with an ethanol-water solution (70 wt% ethanol content) at a material-to-liquid ratio (mass ratio) of 1:10. The extraction time was 1 hour and the extraction was repeated 3 times. The filtrate was filtered through a filter cloth and a 0.45 μm filter membrane. The filtrate was then collected and subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare chamomile extract-2. The total flavonoid content in chamomile extract-2 was ≤10%.

[0041] Preparation of Adiantum capillus-veneris extract-1:

[0042] After pulverizing the maidenhair fern medicinal material, it was extracted by reflux with an ethanol solution (50wt%) at a material-to-liquid ratio (mass ratio) of 1:10, for 1 hour, and three times. The extract was filtered through a filter cloth and a 0.45μm filter membrane. The filtrate was collected and distilled under reduced pressure, then precipitated with an ethanol solution (95wt%). The residue was collected by filtration through a filter cloth, and the solid material was then freeze-dried at low temperature to prepare maidenhair fern extract-1. The polysaccharide content of maidenhair fern extract-1 is ≥8%.

[0043] Preparation of Adiantum capillus-veneris extract-2:

[0044] After pulverizing the maidenhair fern medicinal material, it was extracted by reflux with an ethanol solution (50wt%) at a material-to-liquid ratio (mass ratio) of 1:10, for 1 hour, and three times. The material was filtered through a filter cloth and a filter membrane (45μm) successively. The filtrate was collected and then subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare maidenhair fern extract-2. The polysaccharide content in maidenhair fern extract-2 is ≤8%.

[0045] Preparation of calamus extract-1:

[0046] After pulverizing the *Phyllanthus praecox* herb, it was extracted by reflux with an ethanol solution (70wt%) at a material-to-liquid ratio of 1:10, for 1 hour, and three times. The extract was filtered through a filter cloth and a 0.45μm filter membrane. The filtrate was collected and then eluted with D101 resin using 50% ethanol for 3 BV. The eluent was then subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare *Phyllanthus praecox* extract-1, wherein the total flavonoid content of *Phyllanthus praecox* extract-1 is ≥10%.

[0047] Preparation of calamus extract-2:

[0048] After pulverizing the medicinal material of *Phyllanthus praecox*, it was extracted by reflux with an ethanol solution (70wt%) at a material-to-liquid ratio (mass ratio) of 1:10, for 1 hour, and the extraction was performed 3 times. The material was filtered through a filter cloth and a filter membrane (45μm) successively. The filtrate was collected and then subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare *Phyllanthus praecox* extract-2. The total flavonoid content in *Phyllanthus praecox* extract-2 is ≤10%.

[0049] Preparation of Prunella vulgaris extract-1:

[0050] After pulverizing the herbal medicine, Prunella vulgaris was placed in a supercritical CO2 extraction vessel. The extraction temperature was set at 50℃, the extraction pressure at 30MPa, the separation temperature at 60℃, and the separation pressure at 10MPa. The extraction solvent was an aqueous ethanol solution (ethanol content of 95wt%). After extraction, the filtrate was collected and subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare Prunella vulgaris extract-1. The total triterpenoid content in Prunella vulgaris extract-1 is ≥5%.

[0051] Preparation of Prunella vulgaris extract-2:

[0052] After pulverizing the herbal medicine, Prunella vulgaris was placed in a supercritical CO2 extraction vessel. The extraction temperature was set at 50℃, the extraction pressure at 15MPa, the separation temperature at 60℃, and the separation pressure at 10MPa. The extraction solvent was an aqueous ethanol solution (ethanol content of 95wt%). After extraction, the filtrate was collected and subjected to vacuum distillation using a rotary evaporator to remove the ethanol. The solid material was then freeze-dried at low temperature to prepare Prunella vulgaris extract-2. The total triterpenoid content in Prunella vulgaris extract-2 was ≤5%.

[0053] Example 1

[0054] The composition with skin repair effects in this embodiment includes: 1-15g of chamomile extract, 1-10g of maidenhair fern extract, 1-12g of calamus extract, and 1-8g of prunella vulgaris extract.

[0055] Example 2

[0056] The composition with skin repair effects in this embodiment includes: 1-10g of chamomile extract, 1-12g of maidenhair fern extract, 1-8g of calamus extract, and 1-10g of prunella vulgaris extract.

[0057] Example 3

[0058] The composition with skin repair effects in this embodiment includes: 1-10g of chamomile extract, 1-12g of maidenhair fern extract, 1-10g of calamus extract, and 1-8g of prunella vulgaris extract.

[0059] Example 4

[0060] The composition with skin repair effects in this embodiment includes: 1-12g of chamomile extract, 1-8g of maidenhair fern extract, 1-10g of calamus extract, and 1-8g of prunella vulgaris extract.

[0061] Example 5

[0062] The composition with skin repair effects in this embodiment includes: 1-12g of chamomile extract, 1-12g of maidenhair fern extract, 1-7g of calamus extract, and 1-10g of prunella vulgaris extract.

[0063] Example 6

[0064] The composition with skin repair effects in this embodiment includes: 2-20g of chamomile extract, 1-10g of maidenhair fern extract, 1-8g of calamus extract, and 1-10g of prunella vulgaris extract.

[0065] Example 7

[0066] The composition with skin repair effects in this embodiment includes: 1-10g of chamomile extract, 2-15g of maidenhair fern extract, 1-10g of calamus extract, and 1-5g of prunella vulgaris extract.

[0067] Example 8

[0068] The composition with skin repair effects in this embodiment includes: 1-12g of chamomile extract, 1-10g of maidenhair fern extract, 2-8g of calamus extract, and 1-12g of prunella vulgaris extract.

[0069] Example 9

[0070] The composition with skin repair effects in this embodiment includes: 1-8g of chamomile extract, 1-13g of maidenhair fern extract, 1-9g of calamus extract, and 2-10g of prunella vulgaris extract.

[0071] The preparation methods of the skin-repairing compositions in Examples 1-9 are as follows:

[0072] According to the formula, chamomile extract, maidenhair fern extract, calamus extract, and prunella vulgaris extract were mixed, and then 300g of water and 500g of 1,3-propanediol were added and mixed. The mixture was then processed using a low-temperature microfluidic device. The working temperature of the low-temperature microfluidic treatment was 10℃, and the working pressure was 15000psi. The treatment was repeated three times to prepare a composition with skin repair effects. The particle size of the composition was 70nm.

[0073] Comparative Example 1

[0074] The composition of this comparative example comprises 1-15g of chamomile extract and 1-10g of maidenhair fern extract.

[0075] Comparative Example 2

[0076] The composition of this comparative example comprises 1-15g of chamomile extract and 1-10g of calamus extract.

[0077] Comparative Example 3

[0078] The composition of this comparative example comprises: 1-15g of *Calamus tinctoria* extract and 1-10g of *Prunella vulgaris* extract.

[0079] Comparative Example 4

[0080] The composition of this comparative example comprises: 1-15g of Ampelopsis japonica extract, 1-10g of Achyranthes bidentata extract, and 1-8g of Prunella vulgaris extract.

[0081] To better demonstrate the technical solution provided in this application, the following testing experiments are presented:

[0082] Experiment 1: Antioxidant Activity Test of the Composition

[0083] The antioxidant activity of the compositions prepared in Examples 1-9 and Comparative Examples 1-5 was tested using the following methods:

[0084] Accurately weigh 41.67 mg of DPPH reagent and dilute to 100 mL in a brown volumetric flask. Take 5 mL of the solution and dilute to 50 mL in a brown volumetric flask with anhydrous ethanol to obtain the DPPH test solution (0.1 mmol / L). Transfer 1 mL of the DPPH test solution to each sample of Examples 1-9 and Comparative Examples 1-5 at a concentration of 2 wt%. Mix thoroughly and let stand in the dark for 30 min. Use anhydrous ethanol as a blank control and measure the absorbance at 517 nm. Use vitamin C as a control. Calculate the DPPH scavenging rate of each sample at a concentration of 2 wt% according to the following formula (1).

[0085] (1)

[0087] Where: As—absorbance of the sample solution in the DPPH reaction system; Ar—absorbance of the sample solution in anhydrous ethanol; A0—absorbance of the blank control in anhydrous ethanol.

[0088] The results obtained by the above testing method are shown in Table 1.

[0089] Table 1: Results of Antioxidant Activity Tests on the Compositions

[0090] Sample Name DPPH removal rate Vitamin C 88.56% Example 1 91.35% Example 2 89.45% Example 3 87.26% Example 4 86.47% Example 5 87.78% Example 6 75.43% Example 7 76.25% Example 8 75.57% Example 9 77.58% Comparative Example 1 54.52% Comparative Example 2 50.25% Comparative Example 3 55.08% Comparative Example 4 50.54% Comparative Example 5 56.27%

[0091] As shown in Table 1, the DPPH free radical scavenging rate data of Examples 1-9, which strictly followed the formulation of this invention, exhibited significantly better antioxidant effects than the comparative Examples 1-5, which had formulation defects. This fully verifies the synergistic effect produced by specific formulations. This antioxidant effect helps to resist free radical-mediated cell damage, providing core mechanism support for the composition to exert its skin barrier repair function.

[0092] Experiment 2: Test of the composition's inhibition of the inflammatory factor IL-33

[0093] The inhibitory activity of the inflammatory factor IL-33 in the composition samples prepared in Examples 1-9 and Comparative Examples 1-5 was tested, and the test method is as follows:

[0094] RAW 264.7 cells were used to determine the IL-33 content. The cell suspension was adjusted to an appropriate concentration and seeded into 96-well plates, and cultured overnight at 37°C and 5% CO2. The experiment set up sample groups (containing LPS and the combination of Examples 1-9 and Comparative Examples 1-5), negative control (NC, containing LPS), positive control (PC, containing tranilast and LPS) and blank control (BC, without LPS). After 24 hours of treatment, the culture medium was slowly washed with PBS, and an appropriate amount of PBS was added for repeated freeze-thaw cycles. Then the supernatant was aspirated, centrifuged by vortexing, and the IL-33 content was detected by double antibody sandwich ELISA: standard / sample, biotinylated detection antibody, HRP-labeled streptavidin and TMB substrate were added in sequence, and the absorbance at 450 nm was measured after color development. All experiments were performed in triplicate. The calculation method of IL-33 is shown in Equation (2):

[0095] (2)

[0097] Where: OD sample—absorbance of the sample group; ODBC—absorbance of the blank control group; OD stimulation—absorbance of the negative control group;

[0098] The results obtained by the above testing method are shown in Table 2.

[0099] Table 2: Results of the test for inhibition of the inflammatory factor IL-33 by the composition

[0100] Sample Name IL-33 inhibition rate Trinister 91.92% Example 1 93.83% Example 2 92.20% Example 3 92.67% Example 4 91.45% Example 5 92.69% Example 6 86.43% Example 7 85.57% Example 8 85.43% Example 9 88.40% Comparative Example 1 64.13% Comparative Example 2 60.05% Comparative Example 3 65.38% Comparative Example 4 60.39% Comparative Example 5 66.67%

[0101] Test Example 3: Composition Inhibition of Inflammatory Factor NO Test

[0102] The composition samples prepared in Examples 1-9 and Comparative Examples 1-5 were tested for their inhibitory activity against the inflammatory factor NO. The test methods are as follows:

[0103] RAW 264.7 cell suspension concentration was adjusted and added to 96-well cell plates. The cells were cultured overnight at 37°C and 5% CO2 for detection. NMN was diluted to a non-cytotoxic concentration with LPS-containing cell culture medium and then added. Sample groups consisted of the compositions of Examples 1-9 and Comparative Examples 1-5 added to the above culture medium, respectively. LPS-containing culture medium was used as the negative control (NC), the positive control group (PC) was added with culture medium containing vitamin A and LPS, and LPS-free cell culture medium was used as the blank control (BC). Cells were cultured for 20-24 hours after addition. After the treatment, the content of the inflammatory mediator NO in the supernatant was determined using Griess reagent. The calculation method for NO is shown in formula (3):

[0104] (3)

[0106] In the formula: ODsample—absorbance of the sample group; PC—absorbance of the positive control group.

[0107] The results obtained by the above testing method are shown in Table 3.

[0108] Table 3: Results of the composition's test for inhibiting inflammatory factor NO

[0109] Sample Name Inhibition rate of inflammatory factor NO Example 1 77.33% Example 2 75.14% Example 3 74.23% Example 4 73.56% Example 5 73.37% Example 6 65.25% Example 7 64.36% Example 8 63.45% Example 9 65.86% Comparative Example 1 35.25% Comparative Example 2 37.13% Comparative Example 3 39.12% Comparative Example 4 33.15% Comparative Example 5 32.36%

[0110] Experiment 4: Test on the composition's inhibition of the inflammatory factor IL-6.

[0111] The composition samples prepared in Examples 1-9 and Comparative Examples 1-5 were tested for their inhibitory activity against the inflammatory factor IL-6. The test methods are as follows:

[0112] RAW264.7 cell suspension was adjusted to a concentration and added to a 96-well cell plate. The cells were cultured overnight at 37°C and 5% CO2 for detection. The cells were diluted with LPS-containing cell culture medium to a non-cytotoxic concentration before loading. Sample groups consisted of the compositions of Examples 1-9 and Comparative Examples 1-5, respectively, added to the above culture medium. LPS-containing medium served as the negative control (MC), and LPS-free cell culture medium served as the blank control (BC). Positive controls diluted with LPS-containing cell culture medium served as the positive control (PC). Cells were cultured for 20-24 hours after loading. After the reaction, the supernatant was collected, and the IL-6 content was detected according to the IL-6 ELISA kit operating procedure. The calculation method for IL-6 is shown in equation (4):

[0113] (4)

[0115] Where: T—mean value of inflammatory factor IL-6 in the negative control group; NC—mean value of inflammatory factor IL-6 in the experimental group.

[0116] The results obtained by the above testing method are shown in Table 4.

[0117] Table 4: Test Results of Composition's Inhibition of Inflammatory Factor IL-6

[0118] Sample Name Inhibition rate of the inflammatory factor IL-6 Example 1 84.36% Example 2 83.49% Example 3 84.85% Example 4 82.65% Example 5 81.36% Example 6 69.65% Example 7 62.58% Example 8 68.35% Example 9 69.45% Comparative Example 1 43.05% Comparative Example 2 46.32% Comparative Example 3 45.28% Comparative Example 4 46.25% Comparative Example 5 44.35%

[0119] As shown in Tables 2, 3, and 4, the inhibitory effects of the compositions of this application on key inflammatory factors IL-33, IL-6, and NO strongly validate the accuracy of network pharmacology's prediction of their multi-target anti-inflammatory mechanism. The inhibition rates of Examples 1-9 are significantly better than those of Comparative Examples 1-5, which fully demonstrates that synergistic scientific formulation is the core of enhancing their anti-inflammatory performance.

[0120] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0121] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A composition having skin-repairing efficacy, characterized by comprising, The composition is composed of chamomile extract, adiantum capillus-veneris extract, polygonatum sibiricum extract and prunella vulgaris extract.

2. The composition of claim 1, wherein, The mass fraction of each component in the composition is 5-20 parts of chamomile extract, 8-15 parts of adiantum capillus-veneris extract, 5-10 parts of polygonatum sibiricum extract and 5-15 parts of prunella vulgaris extract.

3. A process for the preparation of a composition according to any one of claims 1-2, characterized in that, The method comprises the following steps: Step one: chamomile, adiantum capillus-veneris and polygonatum sibiricum medicinal materials are crushed, and then extracted by refluxing with ethanol aqueous solution, the extraction time is 1 h, the extraction times is 3, the filtrate is collected by using filter cloth and filter membrane (0.45 μm) in sequence, then the filtrate is distilled by rotary evaporator under reduced pressure to remove ethanol, and the solid material is further freeze-dried at low temperature to prepare chamomile extract, adiantum capillus-veneris extract and polygonatum sibiricum extract; Step two: prunella vulgaris medicinal material is crushed, and then put into supercritical CO2 extraction kettle, the filtrate is collected after extraction, and then distilled by rotary evaporator under reduced pressure to remove ethanol, and the solid material is further freeze-dried at low temperature to prepare prunella vulgaris extract; Step three: chamomile extract, adiantum capillus-veneris extract, polygonatum sibiricum extract and prunella vulgaris extract are mixed, then water and 1,3-butanediol are added and mixed, and then treated by low-temperature microjet equipment, the working temperature of low-temperature microjet treatment is 10℃, the working pressure is 15000 psi, and the treatment is performed for three times to prepare traditional Chinese medicine composition with skin repair effect, and the particle size of the traditional Chinese medicine composition is less than 100 nm.

4. The production method according to claim 3, characterized by, The pressure of supercritical extraction in step two is 20 MPa-40 MPa.

5. The preparation method according to claim 3, characterized in that, The temperature of supercritical extraction in step two is 45℃-55℃.

6. The preparation method according to claim 3, characterized in that, The separation pressure of supercritical extraction in step two is 5 MPa-10 MPa.

7. The preparation method according to claim 3, characterized in that, The separation temperature of supercritical extraction in step two is 30℃-60℃.

8. The preparation method according to claim 3, characterized in that, The working temperature of microjet treatment in step three is 0℃-70℃.

9. The preparation method according to claim 3, characterized in that, The working pressure of microjet treatment in step three is 1000 psi-30000 psi.

10. Use of a composition according to any one of claims 1 to 2, characterized in that The composition is applied to the preparation of cosmetics.

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