Hair-blacking plant extract composition liquid medicine and preparation method thereof

Abstract

The invention discloses a hair-blacking plant extract composition liquid medicine and a preparation method thereof.The liquid medicine is composed of black beans, sesame, dried apricots, oat, kale, palmarosa, a ligusticum wallichii extracting solution, a mulberry extracting solution and auxiliaries, and tyrosine substrates, trace elements and vitamin B family sources are provided to synergistically promote melanin synthesis; dOPC lipid, glycerol and propylene glycol are combined to form a multi-stage permeation system to improve the scalp absorption rate, active ingredients are reserved by adopting mild processes such as vinegar boiling, steaming extraction, cold pressing and wine soaking, alcohol keeps balanced concentration in a finished product so as to take the effects of sterilization, permeation promotion and moisturizing into account, the pH value of the finally prepared liquid medicine is 4.5-6.5, the ethanol content is not more than 8%, and the prepared liquid medicine can be applied to scalp treatment. The shampoo can effectively improve stress and nutritional white hair, enhance hair follicle activity and scalp nutrition circulation, and realize dual effects of melanogenesis recovery and natural blackening and brightening of hair.

Description

Technical Field

[0001] This invention belongs to the technical field of hair darkening and daily chemical health products, and relates to a hair darkening plant extract composition liquid and its preparation method. In particular, it relates to a compound topical liquid that can provide hair follicles with melanin synthesis precursors - tyrosine / its donors, supplement tyrosinase cofactors, such as copper ions and B vitamins, and improve transdermal utilization through a penetration-promoting system. Background Technology

[0002] Hair plays a crucial role in a person's appearance; its color, shine, and density directly impact facial aesthetics and self-confidence. With the fast pace of life, unhealthy lifestyles, and increased environmental stress, problems such as gray hair and hair loss are on the rise across different age groups, with a noticeable trend towards affecting younger people. The causes of gray hair can be broadly categorized into two types: reversible gray hair, primarily caused by acute / chronic stress, malnutrition, medications, or reversible diseases; and irreversible gray hair, mainly caused by genetic factors or age-related follicular aging. Clinical and empirical observations indicate that the former can be reversed to some extent with improved nutrition, stress reduction, or targeted treatment, while the latter is generally difficult to completely reverse with short-term topical medications. Clarifying these etiological differences is a prerequisite for developing effective formulations and treatment outcomes in this field.

[0003] Currently, topical "hair darkening / hair growth" products available on the market and in publicly available literature mainly employ two strategies:

[0004] One type is physical dyeing / dyeing agents, which use pigments or chemical dyes to color the surface of the hair to achieve a short-term effect of covering gray hair;

[0005] The second category is single melanin-promoting or hair growth decoctions, which use Chinese herbal decoctions, essential oils, or single active substances to claim that they promote hair follicle function or melanin synthesis.

[0006] However, these solutions have several technical shortcomings: First, while physical covering can quickly improve appearance, it does not change the melanin synthesis system within the hair follicle, and the color is easily lost after hair loss or shampooing; second, many topical formulas that claim to promote melanin production only provide single plant extracts or essential oils, lacking a holistic formulation and process design that works synergistically from four aspects: "substrate required for melanin synthesis (such as tyrosine) + enzyme cofactor (such as Cu²⁺) + vitamin support (such as B12 / folic acid) + penetration promotion system". Therefore, the actual reversal effect on stress / nutritional gray hair is limited and unstable.

[0007] The aforementioned situation can also be seen in existing published patents and texts. Taking the published topical herbal hair growth and darkening liquid as an example, its formula focuses on the decoction of several traditional Chinese medicines and the combination of essential oils, and uses a comb tool to improve local drug delivery. However, it does not systematically discuss the co-supply of substrate and cofactor, the low-temperature preservation of heat-sensitive vitamins, and the promotion of transdermal absorption by nano / lipids, thus limiting its reproducible efficacy and mechanism support in "reversible gray hair nutritional reversal".

[0008] Furthermore, many existing patents and published documents share common problems at the process level: most employ high-temperature, high-pressure decoction or prolonged water decoction, or ordinary extraction processes, resulting in significant losses of heat-sensitive active components such as vitamins and anthocyanins, which are easily affected by light and heat, thus failing to maximize the retention of melanin-promoting active substances; while hydrophilic precursors (such as tyrosine) and certain inorganic trace elements (such as copper and zinc) are difficult to penetrate the stratum corneum to reach the base of the hair follicle in conventional topical matrices, lacking efficient carriers or adjuvants to improve local utilization and stability. Existing literature and patents have offered solutions with different focuses to address these technical blind spots, but often emphasize only one aspect—such as improving penetration or providing only a nutritional decoction—failing to simultaneously solve the complex problem of penetration, stability, and biological activation using a synergistic four-in-one strategy of "substrate + cofactor + vitamin + carrier." See the published patent for a traditional Chinese medicine formula for head care, which is similar to common practices in the field in terms of ingredients and high-temperature decoction processes, also reflecting the aforementioned shortcomings.

[0009] Therefore, there is an urgent need in the field for a topical composition and preparation method that can retain the activity of heat-sensitive nutrient factors and anthocyanins, provide the substrates and enzyme cofactors required for melanin synthesis, and efficiently deliver these components to the hair follicle basal layer through a low-irritation permeation system.

[0010] Based on the identification of the above-mentioned technical problems, this invention proposes a systematic technical route that combines natural tyrosine sources, vitamin B precursors, trace metal cofactors, and multimodal extraction processes such as low-temperature / alcohol cold soaking, and uses a nano / lipid system composed of lipid auxiliaries and polyols to improve scalp penetration, thereby achieving nutritional reversal of stress-induced and nutritionally reversible gray hair and activation of melanin biosynthesis.

[0011] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0012] To address the shortcomings of existing technologies, this invention provides...

[0013] To achieve the above objectives, the present invention adopts the following technical solution:

[0014] A hair-darkening plant extract composition liquid, comprising, by weight, the following components:

[0015]

[0016] Preferably, the product comprises the following components in parts by weight:

[0017]

[0018] Preferably, the adjuvants include dioleoylphosphatidylcholine (DOPC), glycerol, and propylene glycol.

[0019] Preferably, the DOPC, glycerol and propylene glycol in the adjuvant are in the following mass ratio: mDOPC:mglycerol:mpropylene glycol = (1–5):(5–15):(5–20).

[0020] The present invention also provides a method for preparing the hair-darkening plant extract composition liquid, characterized by comprising the following steps:

[0021] 1) Black bean processing: Take 60-120 portions of black beans and put them into a decoction pot. Add edible rice vinegar with a total weight of 3 times the black beans and soak for 6 hours. Then decoct until the black beans are soft and tender. Cool and filter out the bean residue to obtain black bean juice.

[0022] 2) Sesame pulp: Wash 50-70 parts of sesame seeds, pulp them into a paste, filter and extract the juice;

[0023] 3) Steaming and extraction: Weigh 20-30 portions of dried apricots and the same amount of oats, add 2-3 times the amount of purified water, steam for 30-40 minutes, cool and filter, and keep the supernatant for later use.

[0024] 4) Juicing the whole leaves: 33-48 parts of dried kale and 15-18 parts of palmarosa are crushed after being treated by vibrating sieve and air classifier. Then, an appropriate amount of low-temperature purified water is added, and the juice is extracted in stages while stirring, and the temperature is controlled below 45℃.

[0025] 5) Preparation of Ligusticum chuanxiong extract: Weigh and crush Ligusticum chuanxiong, add 5 times the volume of 70% ethanol, reflux at 60℃ for 2 hours, filter and collect the extract;

[0026] 6) Preparation of cold-soaked mulberry extract: Select fresh or dried mulberries, remove rotten fruits, wash and thoroughly dry them. Place the processed mulberries in a clean, dry, and sealable glass container, and then pour in white wine; the wine should completely submerge the mulberries. After sealing, place in a cool, dark place to age; control the soaking time for 3-5 months, gently shaking once a week during this period, and collect the extract for later use.

[0027] 7) Preparation of compound medicine solution: Mix the above black bean juice, sesame paste, dried apricot and oat liquid, kale and rosehip juice, chuanxiong extract and mulberry extract in proportion, add adjuvants DOPC content of 0.5-3.0%, glycerol content of 2-8% and propylene glycol content of 3-12%, form a nano-dispersion system by mechanical homogenization / ultrasonic emulsification, adjust pH and let stand for aging, filter, sterilize and fill to obtain the finished medicine solution.

[0028] Preferably, the steps include:

[0029] 1) Black bean processing: Take 90-110 parts of black beans and put them into a decoction pot. Add edible rice vinegar with a total weight of 3 times the black beans and soak for 6 hours. Then decoct until the black beans are soft and tender. Cool and filter out the bean residue to obtain black bean juice.

[0030] 2) Sesame pulp: Wash 55-65 parts of sesame seeds, pulp them into a paste, and filter to extract the juice;

[0031] 3) Steaming and extraction: Weigh 22-28 parts of dried apricots and an equal part of oats, add 2-3 times the amount of purified water, steam for 30-40 minutes, cool and filter, and keep the supernatant for later use.

[0032] 4) Juicing the whole leaves: 35-46 parts of dried kale and 16-17 parts of palmarosa are crushed after being treated by vibrating sieve and air classifier. Then, an appropriate amount of low-temperature purified water is added, and the juice is extracted in stages while stirring, and the temperature is controlled below 45℃.

[0033] By adopting the above technical solution, this juicing method can balance the mechanical heat of the pulverizing device, thereby reducing the heat loss of B vitamins and geraniol.

[0034] 5) Preparation of Ligusticum chuanxiong extract: Weigh and crush Ligusticum chuanxiong, add 5 times the volume of 70% ethanol, reflux at 60℃ for 2 hours, filter and collect the extract;

[0035] 6) Preparation of cold-soaked mulberry extract: Select fresh or dried mulberries, remove rotten fruits, wash and thoroughly dry them. Place the processed mulberries in a clean, dry, and sealable glass container, and then pour in white wine; the wine should completely submerge the mulberries. After sealing, place in a cool, dark place to age; control the soaking time for 3-5 months, gently shaking once a week during this period, and collect the extract for later use.

[0036] 7) Preparation of compound medicine solution: Mix the above black bean juice, sesame paste, dried apricot and oat liquid, kale and rosehip juice, chuanxiong extract and mulberry extract in proportion, add adjuvant DOPC 1-2%, glycerin 3-5% and propylene glycol 5-10%, stir evenly, form a nano-dispersion system by mechanical homogenization or ultrasonic emulsification, adjust pH to 4.5-6.5, let stand and age, filter, sterilize and fill to obtain the finished medicine solution.

[0037] Preferably, the total ethanol content of the finished compound solution is ≤8% (v / v).

[0038] Preferably, the ultrasonic treatment in step 7) is performed at 300–600 W with an interval of 5–15 min; or the mechanical homogenization is performed at 100–1000 bar.

[0039] Preferably, the average particle size of the nano-dispersion system obtained in step 7) is 80–400 nm.

[0040] By adopting the above technical solution, the skin penetration of tyrosine and other active ingredients is improved after formulation, thereby increasing the survival rate of melanin.

[0041] The present invention also provides a method for using a hair-darkening plant extract composition liquid, characterized in that it is applied to the scalp, 1-5 mL per application area and gently massaged for 1-3 minutes each time to allow the liquid to be fully absorbed by the scalp; used 1-2 times per night or day, for 2-6 months as one course of treatment.

[0042] Preferably, the composition solution can be applied using a comb kit, the comb kit including a solution chamber and a sponge exudate layer, so as to evenly apply the solution to the scalp.

[0043] The beneficial effects of this invention are:

[0044] 1) Black beans / sesame seeds, as natural sources of tyrosine or its precursors, provide essential substrates for melanin biosynthesis; dried apricots / oats are rich in trace elements such as copper and zinc (or can serve as a supplementary source of trace copper); kale, with its B9 / B12 precursors preserved through low-temperature extraction, supports hair follicle cell division and DNA synthesis, thus providing a nutritional foundation for reversible gray hair restoration.

[0045] 2) Geraniol, as a natural terpene, increases the fluidity of the stratum corneum; DOPC forms a lipophilic phase that encapsulates hydrophilic substrates and also acts as a membrane phase aid; polyols improve the hydrophilic / lipophilic balance and act as a penetration aid; the three work synergistically and are prepared into nano-dispersions through ultrasonic / high pressure homogenization, which significantly improves the local utilization rate of tyrosine and trace elements into the hair follicle basal layer.

[0046] 3) Ligusticum chuanxiong can promote local microcirculation and dilate capillaries, which is beneficial to the supply of nutrients to hair follicles; mulberry is rich in anthocyanins and other strong antioxidants, which can reduce the damage of oxidative stress to melanocytes. The combined effect of the two helps to delay the formation of new gray hair and improve the microenvironment of hair follicles.

[0047] 4) While using alcohol to improve the dissolution and preservation of certain ingredients, the alcohol content in the finished product is limited to a mild range. Combined with the moisturizing effects of glycerin and propylene glycol, it can not only improve greasiness in the short term and provide antibacterial assistance, but also avoid long-term dryness or irritation.

[0048] In summary, this invention systematically combines natural tyrosine sources (such as black beans and sesame seeds) with trace element sources (such as dried apricots and oats) and B vitamin sources (such as kale) to construct a synergistic compound topical hair-darkening preparation with a "substrate-cofactor-vitamin-penetration" mechanism. Furthermore, it improves hair follicle microcirculation and provides antioxidant protection through extracts of Ligusticum chuanxiong and mulberry. The extraction process utilizes low-temperature treatment and alcohol cold soaking, and the alcohol content in the finished product is controlled to ≤8% v / v to balance ingredient preservation, sterilization, and moisturizing. By setting the pH to 4.5–6.5 (close to the skin's slightly acidic pH) and employing a low concentration of natural terpenes and a glycerin / propylene glycol moisturizing system, the risk of irritation is reduced, making it suitable for frequent topical application. This invention has significant advantages in nutritional reversal of stress / nutritional (reversible) gray hair, activation of melanin synthesis, low irritation, and industrial-scale production feasibility. Detailed Implementation

[0049] The present invention will be further described below through specific embodiments. To make the inventive objectives, technical solutions, and beneficial technical effects of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the embodiments described in this specification are merely for explaining the present invention and are not intended to limit the present invention.

[0050] Unless otherwise stated, all instruments and reagents used in the examples are commercially available or synthesized using conventional methods and can be used directly without further processing, and all instruments used in the examples are commercially available.

[0051] Instruments and reagents:

[0052] Suppliers: Sigma-Aldrich, Merck, Thermo Fisher, Aladdin (China);

[0053] Analytical reagents and standards:

[0054] L-Tyrosine Standard (≥98%)

[0055] Methylcobalamin (Vitamin B12) Standard

[0056] L-DOPA (tyrosinase substrate)

[0057] Methanol, acetonitrile (HPLC grade)

[0058] PBS, DMEM / RPMI medium, FBS, penicillin and streptomycin

[0059] CCK-8 or MTT kit

[0060] Melanin extraction buffer (NaOH 10% solution, etc.)

[0061] Microporous filters (0.45 μm, 0.22 μm), syringe filters

[0062] Sterile sampling tubes, centrifuge tubes (15 / 50 mL)

[0063] Consumables:

[0064] Franz diffusion cells

[0065] DLS sampling vials

[0066] High-pressure homogenizer / Ultrasonic cell disruptor (Qsonica);

[0067] High-pressure homogenizer (Microfluidizer M-110P);

[0068] Analytical balance, ultrasonic cleaning pot, magnetic stirrer, constant temperature water bath, steamer, reflux device

[0069] HPLC / LC-MS instruments (Agilent 1260 / 1290, Thermo Dionex);

[0070] UV / Vis spectrophotometer, microplate reader (BioTek Synergy H1);

[0071] DLS particle analyzer (Malvern Zetasizer Nano ZS);

[0072] Centrifuges, refrigerators / ultra-low temperature incubators, constant temperature incubators, CO2 incubators;

[0073] Franz diffusion cell: PermeGear Standard Franz Cells (receiver cell volume and exposed area record).

[0074] High-pressure homogenization: Microfluidizer M-110P or Avestin Emulsiflex;

[0075] Ultrasonic processor: Qsonica Q500;

[0076] DLS: Malvern Zetasizer Nano ZS;

[0077] HPLC: Agilent 1260 / 1290, UV / Diode array detector; LC-MS can be used for trace detection;

[0078] Microplate reader: BioTek Synergy H1;

[0079] Source of ex vivo skin: pig ear skin epidermis;

[0080] Example 1:

[0081] Formula (based on 1 g = 1 part, the overall example formula is based on a final liquid scale of approximately 1 kg): 60 parts black beans, 50 parts sesame seeds, 20 parts dried apricots, 20 parts oats, 33 parts kale, 15 parts dried palm folia, 100 parts Sichuan lovage extract, 120 parts mulberry extract, and 30 parts total of adjuvants (of which DOPC 0.5% w / w, glycerin 2% w / w, and propylene glycol 3% w / w, the adjuvants are approximately in a mass ratio of mDOPC:mglycerin:mpropylene glycol = 1:4:6;

[0082] Preparation steps:

[0083] 1) Black bean processing: Take 60 parts of black beans, add 180 parts of edible rice vinegar and soak for 6 hours. Then place them in a decoction pot and heat over low heat to 95–100 ℃ and decoct for 40–45 minutes. Cool to <40 ℃ and filter with gauze to obtain black bean juice for later use.

[0084] 2) Sesame paste: Wash and dry 50 parts of sesame seeds, then grind them into a paste and filter to obtain the supernatant;

[0085] 3) Steaming and extraction: Put 20 parts dried apricots and 20 parts oats into a steamer, add 2-3 times the amount of purified water and steam for 30-40 minutes. Cool, filter and take the supernatant.

[0086] 4) Juicing whole leaves: Crush 33 parts kale and 15 parts rose grass and add cold purified water. Juice in stages, controlling the temperature below 45 ℃, and filter to obtain the juice.

[0087] 5) Ligusticum chuanxiong extract and mulberry extract: 100 parts of the pre-prepared Ligusticum chuanxiong extract were extracted and concentrated by reflux with 60% ethanol; and 120 parts of mulberry extract were soaked in white wine for 3 months for later use.

[0088] 6) Preparation of compound drug solution: Black bean juice, sesame paste, dried apricot and oat liquid, kale and rosehip juice, chuanxiong and mulberry extract are mixed according to the ratio, and DOPC, glycerin and propylene glycol are added, with the corresponding contents of approximately 0.5%, 2% and 3% of the final system, respectively. The mixture is premixed with a high shear disperser for 5-10 min.

[0089] The ultrasonic treatment conditions were as follows: 300 W, 5 min intervals × 3 rounds, with a total cumulative time of about 15 min. Intervals were used to prevent overheating, and the target average particle size was controlled at 80–400 nm.

[0090] pH adjustment and aging: Adjust the pH of the finished product to 4.5–6.5 and let it stand for 24–72 h to facilitate the mutual dissolution and stability of the components; after 0.45 μm pre-filtration, sterilize at 60 ℃ for 30 min and fill into brown bottles. The total ethanol content of the finished product is controlled to ≤8% (v / v).

[0091] Instructions for use: Apply 1–3 mL to the area to be treated each time, gently massage for 1–3 minutes to allow the solution to be fully absorbed by the scalp; use 1–2 times nightly or daily, and observe the effect after continuous use.

[0092] Example 2:

[0093] Example formula: 100 parts black beans, 60 parts sesame seeds, 25 parts dried apricots, 25 parts oats, 40 parts kale, 16 parts palmarosa, 110 parts chuanxiong extract, 135 parts mulberry extract, and a total of 40 parts of adjuvants, including 1.5% w / w DOPC, 4% w / w glycerin, and 7% w / w propylene glycol;

[0094] Operating steps:

[0095] 1) Black bean pretreatment: Weigh 100 parts of black beans, add 300 parts of rice vinegar and soak for 6 hours, simmer for 45 minutes, cool and filter to obtain black bean juice;

[0096] 2) Sesame paste: Cold grind 60 parts sesame seeds and filter to obtain sesame paste;

[0097] 3) Steaming and extraction: Add 25 parts dried apricots and 25 parts oats to water and steam for 30–40 minutes. Cool and filter to obtain the supernatant.

[0098] 4) Juicing whole leaves: Juice 40 parts kale and 16 parts palmarosa at low temperature (≤45 ℃) and filter;

[0099] 5) Ligusticum chuanxiong extraction and mulberry extract: Add 5 times the volume of 70% ethanol to 110 parts of the pre-prepared Ligusticum chuanxiong extract, reflux at 60℃ for 2 hours, filter and collect the extract; and soak 135 parts of mulberry extract in 68-degree high-proof liquor for 4 months for later use.

[0100] 6) Composite and nano-scale: Mix the extracts according to the ratio, add DOPC (1.5%), glycerol (4%), and propylene glycol (7%), and use a high-pressure homogenizer at 500 bar for 2-3 cycles to form a stable nano-dispersion system; the target average particle size is 100-200 nm, and the resulting system has a uniform appearance;

[0101] 7) pH and sterilization: Adjust pH to 5.0, let stand for 48 h for aging, filter with 0.45 μm, and then use low temperature sterilization or aseptic filling process. Fill and label, and the total ethanol content of the finished product is ≤8% (v / v).

[0102] Example 3:

[0103] Example formula: 120 parts black beans, 70 parts sesame seeds, 30 parts dried apricots, 30 parts oats, 48 ​​parts kale, 18 parts palmarosa, 120 parts chuanxiong extract, 150 parts mulberry extract, and 50 parts total of adjuvants. Use 2.5% w / w DOPC, 8% w / w glycerin, and 12% w / w propylene glycol. The adjuvant mass ratio is mDOPC:mglycerin:mpropylene glycol = 1:3.2:4.8.

[0104] Preparation steps:

[0105] 1) Black bean processing: Soak 120 parts black beans in 360 parts rice vinegar for 6 hours, simmer for 50-60 minutes, cool and filter to obtain black bean juice;

[0106] 2) Sesame pulping and steaming process: Sesame pulping (70 parts) and steaming of dried apricots / oats (30 parts each, steaming for 30–40 min) are carried out according to the steps in claim 5.

[0107] 3) Low-temperature juicing of whole leaves: Juice 48 parts of kale and 18 parts of palmarosa at low temperature (≤45 ℃) to avoid degradation of vitamin B and geraniol;

[0108] 4) Ligusticum chuanxiong and mulberry: The 70% ethanol extract concentrate of Ligusticum chuanxiong obtained in advance as described in the claims and the mulberry liquor extract were aged for 5 months and then added and mixed.

[0109] 5) Nanoforming: After mixing the liquids, add a high content of DOPC, glycerol, and propylene glycol, and perform strong emulsification nanoforming by mechanical homogenization at 700–1000 bar for 2 cycles; the target average particle size is controlled at 100–300 nm, and the system is dispersed stably.

[0110] 6) pH adjustment and finished product control: Adjust the pH of the system to 5.5, let it stand for 24–72 h, filter it with 0.45 μm, perform microbial limit testing, and fill it under compliant conditions. The total ethanol content of the final product is ≤8% (v / v).

[0111] Comparative Example 1: Black bean decoction only (naked liquid).

[0112] Comparative Example 2: Formulation in the prior art

[0113] A topical herbal hair growth and darkening liquid is made from the following components: 40-60 parts ginger, 40-60 parts mugwort, 70-100 parts arborvitae leaves, 15-25 parts safflower, 20-40 parts angelica dahurica, 20-40 parts tribulus terrestris, 50-80 parts processed he shou wu, and 80-120 parts black beans.

[0114] Examples 1-3 and Comparative Examples 1-2 were performed as follows: A. Skin penetration (Franz cell in vitro penetration); B. Cellular functional verification (tyrosinase activity, melanin production); C. Comparison of simulated clinical / human observation indicators with skin tolerance tests.

[0115] Experimental methods:

[0116] The skin sample was placed in a Franz cell (corne side out), the receiving chamber was filled with PBS, and pre-equilibrated for 30 min;

[0117] Apply an equal amount of sample (equal active ingredient or equal volume, such as 200 μL) to the stratum corneum of the donor pool;

[0118] Sampling: 0, 1, 2, 4, 8, 24 h. At each time point, a certain volume of the receiving solution was taken (and replaced with an equal volume of fresh PBS), and stored at −20 ℃ for analysis.

[0119] After 24 hours, the skin sample was removed and the surface was washed (the eluent was recorded). Then, the epidermis / dermis was separated (hot water method or enzymatic hydrolysis method) and weighed. The epidermis / dermis / hair follicle area was dissolved (e.g., NaOH extraction or organic solvent extraction) and the key components were quantified.

[0120] The results are shown in the table below:

[0121] 24-hour cumulative transmittance % Tyrosinase activity Increased melanin content % 3-month reversal rate % Example 1 22.1 1.8 18 45 Example 2 35.3 2.4 30 62 Example 3 42.0 2.9 38 70 Comparative Example 1 5.0 1.1 4 10 Comparative Example 2 12.0 1.6 12 40

[0122] As can be seen from the table, the nano / lipid system (Ex1–Ex3) of this invention significantly improves the penetration rate of hydrophilic tyrosine and related small molecules to the dermis / hair follicle, and the effect increases in a dose-dependent manner with the increase of DOPC / adjuvant content and nano-intensity. The formulation of this invention can significantly enhance tyrosinase activity and melanin biosynthesis, and the nanolipid encapsulation and co-supply of trace copper / vitamins make the Ex2 / Ex3 effect superior to the empty or traditional compound formulation. Compared with black bean extract alone or traditional compound formulations, this invention significantly improves the penetration rate of key active ingredients to hair follicles, tyrosinase activity and melanin synthesis through the synergistic strategy of "tyrosine substrate + trace elements + B vitamins + multi-level penetration / nanolipid carrier", and shows a higher visible reversal rate in simulated human use, while maintaining good skin tolerance.

[0123] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A hair-growing plant extract composition liquid, characterized by comprising: By weight parts, comprising the following components: ​ 2. The hair-growing plant extract composition liquid according to claim 1, characterized by, By weight parts, comprising the following components:

3. The hair-growing plant extract composition liquid according to claim 1, characterized by, The adjuvant comprises dioleoylphosphatidylcholine DOPC, glycerol, and propylene glycol.

4. The hair-growing plant extract composition liquid according to claim 3, characterized by, The DOPC, glycerol, and propylene glycol in the adjuvant are in a mass ratio of mDOPC:mglycerol:mpropylene glycol = (1-5):(5-15):(5-20).

5. A method of preparing a hair restorer composition liquid according to any one of claims 1 to 4, characterized in that, Comprising the steps of: 1) black bean processing: take 60-120 parts of black beans into the decoction pot, add 3 times the amount of edible vinegar to the total weight of black beans for 6 hours, then decoct until the black beans are soft, cool, filter out the bean dregs to obtain black bean juice; 2) sesame pulp beating: 50-70 parts of sesame is washed and beaten into pulp, and the juice is filtered; 3) steam extraction: take 20-30 parts of dried apricot and the same amount of oat, add 2-3 times of pure water, cook for 30-40 minutes, cool and filter, take the supernatant for standby; 4) raw leaf juice beating: dry 33-48 parts of kale and 15-18 parts of rose grass are treated by vibration screening and airflow winnowing, then crushed, and then added with appropriate amount of low-temperature pure water, and the juice is squeezed in stages while stirring, and the temperature is controlled below 45℃; 5) chuanxiong extract preparation: take chuanxiong powder, add 5 times the volume of 70% ethanol, and reflux extract at 60℃ for 2 hours, filter and take the extract; 6) mulberry cold soaking extract preparation: select fresh or dried mulberries, remove rotten fruits, wash and dry thoroughly, put the treated mulberries into clean, dry and sealable glass containers, then pour in white wine; the wine should completely immerse the mulberries; seal and place in a cool and dark place for aging; control the soaking time for 3-5 months, shake once a week during the period, and take the extract for standby; 7) compound liquid preparation: mix the above black bean juice, sesame pulp, apricot oat liquid, kale rose grass juice, chuanxiong extract and mulberry extract according to the proportion, add adjuvant DOPC content of 0.5-3.0%, glycerol content of 2-8%, and propylene glycol content of 3-12%, form a nano dispersion system by mechanical homogenization / ultrasonic emulsification, adjust pH and stand for aging, filter, sterilize and fill to get the finished product.

6. The method of claim 5, wherein the hair growth plant extract solution is prepared by adding 0.1 to 1% of the hair growth plant extract solution to 99 to 100% of the water. Comprising the steps of: 1) black bean processing: take 60-120 parts of black beans into the decoction pot, add 3 times the amount of edible vinegar to the total weight of black beans for 6 hours, then decoct until the black beans are soft, cool, filter out the bean dregs to obtain black bean juice; 2) sesame pulp beating: 50-70 parts of sesame is washed and beaten into pulp, and the juice is filtered; 3) steam extraction: take 20-30 parts of dried apricot and the same amount of oat, add 2-3 times of pure water, cook for 30-40 minutes, cool and filter, take the supernatant for standby; 4) raw leaf juice beating: dry 33-48 parts of kale and 15-18 parts of rose grass are treated by vibration screening and airflow winnowing, then crushed, and then added with appropriate amount of low-temperature pure water, and the juice is squeezed in stages while stirring, and the temperature is controlled below 45℃; 5) chuanxiong extract preparation: take chuanxiong powder, add 5 times the volume of 70% ethanol, and reflux extract at 60℃ for 2 hours, filter and take the extract; 6) mulberry cold soaking extract preparation: select fresh or dried mulberries, remove rotten fruits, wash and dry thoroughly, put the treated mulberries into clean, dry and sealable glass containers, then pour in white wine; the wine should completely immerse the mulberries; seal and place in a cool and dark place for aging; control the soaking time for 3-5 months, shake once a week during the period, and take the extract for standby; 6) Preparation of mulberry cold extraction liquid: Select fresh or dried mulberries, remove rotten fruits, and wash and thoroughly dry the water. Put the treated mulberries into a clean, dry, and sealable glass container, then pour in the white wine; the wine should completely immerse the mulberries; seal and store in a cool, dark place; control the soaking time for 3-5 months, shake gently once a week during the period, and take the extraction liquid for use; 7) Preparation of composite liquid: Mix the black bean juice, sesame paste, apricot dried oat liquid, kale rose grass juice, and chuanqiong extraction liquid according to the proportion, add the auxiliary DOPC 1-2%, glycerol 3-5%, and propylene glycol 5-10%, stir evenly, form a nano dispersion system by mechanical homogenization or ultrasonic emulsification, adjust the pH to 4.5-6.5, and stand for aging, filter, sterilize, and fill to obtain the finished product liquid.

7. The method of claim 5, wherein the hair growth plant extract solution is prepared by adding 0.1 to 1% of the hair growth plant extract solution to 99 to 100% of the water, and then adding 0.1 to 1% of the hair growth plant extract solution to the mixture. The total ethanol content of the finished product of the composite liquid is ≤8% (v / v).

8. The method of claim 5, wherein the preparation method of the external hair-growing plant extract solution is characterized by, The ultrasonic treatment condition in step 7) is 300-600 W, intermittently for 5-15 min; or the mechanical homogenization condition is 100-1000 bar.

9. The method of claim 8, wherein the preparation method of the external hair-growing plant extract solution is characterized by, The average particle size of the nano dispersion system prepared in step 7) is 80-400 nm.

10. A method for using a hair-growing plant extract composition liquid, characterized by, Apply to the scalp area, 1-5 mL per area, and gently rub for 1-3 min, so that the liquid is fully absorbed by the scalp; the frequency is 1-2 times per night or per day.

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