Magnetic control double-effect intelligent dressing, preparation method thereof and chronic wound treatment device

Abstract

The invention belongs to the technical field of dressings, and discloses a magnetic control double-effect intelligent dressing, a preparation method thereof and a chronic wound treatment device, the magnetic control double-effect intelligent dressing comprises an antibacterial fabric and a conductive coating; the preparation method comprises the following steps: reducing antibacterial metal ions on the surface of a fabric by using a reducing compound to obtain an antibacterial fabric, depositing a conductive coating on the surface of the antibacterial fabric to obtain the magnetic control double-effect intelligent dressing, and after reducing the antibacterial metal ions by using the reducing compound, converting the reducing compound into a polymer to obtain the magnetic control double-effect intelligent dressing. The antibacterial metal ions are converted into antibacterial metal nanoparticles, and the polymer wraps the surfaces of the antibacterial metal nanoparticles; the treatment device comprises a rotating magnetic field generator and the magnetic control double-effect intelligent dressing. The rotating magnetic field generator is used for generating a rotating magnetic field, and the magnetic control double-effect intelligent dressing is located in the rotating magnetic field. According to the invention, the behaviors of cell proliferation, migration, differentiation and the like can be regulated and controlled, the release of antibacterial metal ions of the dressing can be regulated and controlled as required, and the antibacterial performance of the dressing is enhanced.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of dressings, and relates to a magnetic control dual-effect intelligent dressing, a preparation method thereof and a chronic wound treatment device. BACKGROUND

[0002] Chronic wounds refer to wounds that cannot complete healing within the normal healing period (usually more than 4 weeks) due to various reasons, such as wounds that are in an inflammatory or repair stagnation state for a long time. The treatment of chronic wounds is a major and increasingly prominent clinical challenge, as the complex pathological microenvironment of chronic wounds is characterized by persistent infection, chronic inflammation and interruption of biological electrical signals, and the situation is becoming more and more serious. Traditional dressings only serve as passive barriers, and it is difficult to cope with the complex infection microenvironment, persistent inflammation and damaged biological electrical signals of chronic wounds, leading to stagnation of the healing process and a heavy burden on the clinic.

[0003] In view of these characteristics of chronic wounds, it is necessary to develop a new type of dressing. Such a dressing not only needs to be able to cooperate with other equipment to achieve controlled release of antibacterial metal ions, but also needs to have the function of producing an electrotherapy effect. However, the current situation is not optimistic. Silver nanoparticles (AgNPs) are widely used for anti-infection due to their broad-spectrum antibacterial properties, but the traditional manufacturing and loading methods will cause uncontrolled release of silver ions, which not only hinders the healing process but also damages the newly formed tissue, greatly limiting their application in high-performance wound dressings. Although electrical stimulation therapy is an effective method for promoting wound healing and regeneration, it relies on bulky wired equipment, which severely limits patient compliance and treatment convenience. Therefore, the existing dressing technology cannot meet the above requirements, and it is an urgent and unmet need to develop an integrated platform that combines wireless power supply, on-demand electrical stimulation and precise control of antibacterial function. This system is expected to become a revolutionary solution to break the current bottleneck in chronic wound management.

[0004] To solve the above problems, the patent application with the publication number CN120420155A discloses an intelligent bandage integrating wound monitoring, drug delivery and electrical stimulation, which uses a portable battery to power a portable control module, and a hydrogel composed of an electrically responsive polymer. The charged groups or ions in the molecular chain of the polymer will undergo directional migration or conformational change under the action of an electric field, causing the swelling / contraction or degradation of the hydrogel, thereby releasing the loaded drugs. By modulating the electrode with a specific intensity of direct current or pulse voltage, the response behavior of the hydrogel can be accurately controlled. However, this method of releasing drugs by modulating the electrode with a specific intensity of direct current or pulse voltage to control the change in the shape of the hydrogel is prone to cause the hydrogel to lose water and become hard in the actual environment, which hinders the release of drugs, and the voltage for electrical stimulation and drug release may have a contradictory window, which also leads to poor recovery of the wound.

[0005] Patent application CN119113178A discloses a self-generating medical dressing with a self-pumping function and its preparation method. This self-generating medical dressing includes a power-generating layer and an exudate-absorbing and moisture-retaining layer sequentially arranged. The power-generating layer includes a substrate in which a wet-electric effect active substance and an electrode material are distributed. It has a self-generating function, utilizing the flow of exudate through the conductive layer to generate an electrical signal to apply electrical stimulation to the wound. However, the power generation using the wet-electric effect depends on wound exudate. When the wound dries, no liquid flows through the dressing, no electrical stimulation is generated, and the electrotherapy effect is ineffective.

[0006] Patent application CN119523733A discloses an electroactive wound dressing system based on magnetoelectric technology, its preparation method, and its application. It utilizes a high-frequency current passing through a transmitting coil to generate a high-frequency magnetic field around a backpack. This magnetic field, through electromagnetic induction, induces a high-frequency current in a liquid metal receiving coil within the magnetoelectric composite electroactive wound dressing, which then acts on the wound. However, the significant heat loss from the coil, which generates the current, may lead to accelerated evaporation of the hydrogel, affecting the generation of the electrical signal. Furthermore, this method only demonstrates a magnetoelectric effect, without revealing any magnetothermal-antibacterial effects.

[0007] Patent application CN119971111A discloses an antibacterial dressing based on the synergistic effect of magnetorheology and magnetothermal processes, and its preparation method. Under the magnetorheological effect, the morphology and stiffness of the dressing surface change, forming an outward-facing needle-like array structure. This structure has the ability to capture and destroy bacteria, thereby eliminating them and solving the problem that existing antibacterial dressings easily lose their antibacterial effect due to bacterial mutation and drug resistance. It utilizes the movement and deformation of magnetic particles in a magnetic field to destroy the bacterial structure through force. However, this antibacterial mechanism is contact-based sterilization, ineffective against bacteria that cannot be contacted, and may have cytotoxicity issues. The sharp structure also leads to massive cell death, and it does not demonstrate the effect of the magneto-electric effect in promoting wound healing.

[0008] Therefore, it is of great significance to develop a magnetically controlled dual-effect intelligent dressing, its preparation method, and a treatment device for chronic wounds to solve the above problems. Summary of the Invention

[0009] The purpose of this invention is to solve the problems existing in the prior art and to provide a magnetically controlled dual-effect intelligent dressing, its preparation method, and a treatment device for chronic wounds.

[0010] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0011] A method for preparing a magnetically controlled dual-effect (electrotherapy and antibacterial) smart dressing involves first reducing antibacterial metal ions on the surface of a fabric using a reducing compound to obtain an antibacterial fabric, and then depositing a conductive coating on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect smart dressing. In this method, after the reducing compound reduces the antibacterial metal ions, the reducing compound transforms into a polymer, and the antibacterial metal ions transform into antibacterial metal nanoparticles. The polymer coats the surface of the antibacterial metal nanoparticles, which on the one hand prevents the explosive release of antibacterial metal ions and improves the safety of the dressing, and on the other hand acts as an adhesive to improve the interfacial interaction between the fabric, the antibacterial metal nanoparticles, and the conductive coating, thereby optimizing conductivity and stability.

[0012] As a preferred technical solution:

[0013] The preparation method of the magnetically controlled dual-effect intelligent dressing described above uses cotton gauze as the fabric, with a thickness of 0.3-0.5 mm. The reducing compound is dopamine, 5-hydroxytryptophan, or tannic acid, and the antibacterial metal ion is silver ion or gold ion.

[0014] The preparation method of the magnetically controlled dual-effect intelligent dressing described above involves the following process: First, the fabric is immersed in a weakly alkaline solution (which can be composed of NH3·H2O, anhydrous ethanol and water, or can be composed of Tris buffer, carbonate buffer, borate buffer, phosphate buffer and glycine buffer at pH=8.5) for 15-60 minutes at room temperature. Then, a solution containing reducing compounds and antibacterial metal salts is added to the solution. After stirring in the dark at room temperature for 1-5 hours, the fabric is removed and post-treated (rinsed repeatedly with water and dried overnight at 50°C).

[0015] The preparation method of the magnetically controlled dual-effect intelligent dressing described above involves a solution containing a reducing compound and an antibacterial metal salt, wherein the concentration of the reducing compound is 9-36 mM and the concentration of the antibacterial metal salt is 59-236 mM; the mass-to-volume ratio of the fabric, the weakly alkaline solution, and the solution containing the reducing compound and the antibacterial metal salt is 1.2-2.4 g: 37.67-75.34 mL: 5-10 mL.

[0016] In the preparation method of the magnetically controlled dual-effect smart dressing described above, the conductive coating is a polypyrrole coating, a PEDOT coating, a polytriphenylamine coating, a polycarbazole coating, or a polyaniline coating.

[0017] The preparation method of the magnetically controlled dual-effect smart dressing described above involves depositing a conductive coating on the surface of an antibacterial fabric as follows: first, a mixture containing an oxidant, an antibacterial fabric, and water is frozen at -20°C until solidified; then, a solution containing a conductive monomer is dropped onto the ice surface and reacted at 4°C for 12-24 hours.

[0018] In the preparation method of the magnetically controlled dual-effect intelligent dressing described above, the oxidant is ferric nitrate nonahydrate, ferric chloride hexahydrate, anhydrous ferric chloride, potassium persulfate, or ammonium persulfate.

[0019] In the preparation method of the magnetically controlled dual-effect intelligent dressing described above, the concentration of the oxidant in the mixture is 30.1-120.4 mM, the concentration of the antibacterial fabric is 1.2-2.4 g / 20 mL, the concentration of the solution containing the conductive monomer is 0.36-1.44 M, and the volume ratio of the mixture to the solution containing the conductive monomer is 1:1-2.

[0020] The present invention also provides a magnetically controlled dual-effect intelligent dressing, which is prepared by the method described above.

[0021] The present invention also provides a treatment device for chronic wounds, including a rotating magnetic field generator and a magnetically controlled dual-effect intelligent dressing as described above; the rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0022] Based on the principle of electromagnetic induction, the magnetically controlled dual-effect intelligent dressing can generate wireless induction microcurrents under a rotating magnetic field. On the one hand, the wireless induction microcurrents can regulate cell proliferation, migration and differentiation, and on the other hand, the wireless induction microcurrents can generate heat, promote the release of antibacterial metal ions, and enhance the antibacterial properties of the dressing.

[0023] Furthermore, by changing the duration of magnetic field stimulation, the release of antibacterial metal ions in the dressing can be controlled as needed, thereby enabling controllable antibacterial performance, reducing the uncontrollable hindering of wound healing by antibacterial metal ions, and achieving rapid healing and functional recovery of chronic wounds infected with bacteria.

[0024] As a preferred technical solution:

[0025] The chronic wound treatment device described above includes a rotating magnetic field generator comprising a motor and a neodymium iron boron permanent magnet, the neodymium iron boron permanent magnet being fixed on the output shaft of the motor; the rotational speed of the rotating magnetic field is 300-600 rpm, and the magnetic induction intensity is 90-100 mT.

[0026] This invention also provides a treatment method for chronic wounds, employing a chronic wound treatment device as described above; the specific process is as follows: first, a full-thickness skin defect animal model with a diameter of 10 mm is created on a male rat (weighing 300-500g); then, 100 μL of Staphylococcus aureus suspension (with a Staphylococcus aureus concentration of 1×10⁻⁶) is dripped into the wound. 6After soaking the wound with CFU / mL solution for 30 minutes, the wound was bandaged with medical gauze and tape. After 24 hours, a chronic wound model with bacterial infection was obtained. Then, the magnetically controlled dual-effect intelligent dressing was applied to the chronic wound model. From day 1 to day 7, the rotating magnetic field generator was activated for 1 hour each day. The main purpose was to promote the release of antibacterial metal ions, achieve rapid killing of bacteria, and reduce infection. From day 8 onwards, the rotating magnetic field generator was activated for 0.25 hours each day. The main purpose was to generate microcurrents to regulate the proliferation and differentiation of cells such as fibroblasts, macrophages, endothelial cells, and nerve cells, and promote rapid wound healing and functional reconstruction.

[0027] Beneficial effects:

[0028] (1) By coating the surface of antibacterial metal nanoparticles with polymer, this invention can prevent the explosive release of antibacterial metal ions and improve the safety of dressings. On the other hand, it can act as an adhesive to improve the interfacial interaction between fabric, antibacterial metal nanoparticles and conductive coating, thereby optimizing conductivity and stability.

[0029] (2) Based on the principle of electromagnetic induction, the magnetically controlled dual-effect intelligent dressing can generate wireless induction microcurrent under a rotating magnetic field. On the one hand, the wireless induction microcurrent can regulate cell proliferation, migration and differentiation. On the other hand, the wireless induction microcurrent can generate heat, promote the release of antibacterial metal ions, and enhance the antibacterial performance of the dressing.

[0030] (3) The present invention can control the release of antibacterial metal ions in dressings as needed by adjusting the time of magnetic field stimulation, thereby making the antibacterial performance controllable, reducing the uncontrollable obstacles of antibacterial metal ions to wound healing, and realizing the rapid healing and functional recovery of chronic wounds infected by bacteria. Attached Figure Description

[0031] Figure 1 This is a TEM image of the antibacterial fabric prepared in Example 1 of the present invention; in the image, AgNPs are silver nanoparticles and PDA is polydopamine.

[0032] Figure 2 The image shows the microstructure of the magnetically controlled dual-effect smart dressing prepared in Example 1 of this invention. In the image, a is a SEM image of the magnetically controlled dual-effect smart dressing, b is a magnified SEM image of a portion thereof, and c is a TEM image of the antibacterial metal nanoparticles containing a conductive coating.

[0033] Figure 3 The graph shows the current output of the magnetically controlled dual-effect intelligent dressing prepared in Embodiment 1 of the present invention, tested using a digital source meter.

[0034] Figure 4Infrared images of chronic wounds treated in control group 1, control group 2, comparative example 1 and example 1 of this invention under a rotating magnetic field;

[0035] Figure 5 The temperature change curves under a rotating magnetic field are shown for the treatment of chronic wounds in control group 1, control group 2, comparative example 1 and example 1 of this invention.

[0036] Figure 6 This is a schematic diagram of the temperature rise under a rotating magnetic field during the treatment of chronic wounds using COMSOL simulation in Comparative Example 1 and Example 1; in the figure, a is the temperature thermogram of the cross section of Comparative Example 1, and b is the temperature thermogram of the cross section of Example 1.

[0037] Figure 7 The temperature curves under a rotating magnetic field are shown in Comparative Example 1 and Example 1 of the present invention during the treatment of chronic wounds in Comparative Example 1 and Example 1 of the present invention.

[0038] Figure 8 This is a schematic diagram showing the results of ICP-OES testing during the treatment of chronic wounds in Comparative Example 1 and Example 1 of the present invention;

[0039] Figure 9 The images show the effects of wound healing and functional recovery in a Staphylococcus aureus-infected male rat model of full-thickness skin defect during the treatment of chronic wounds in Examples 1, 1, 2, 1, and 2 of the present invention.

[0040] Figure 10 This is a bar chart showing the wound healing rate on day 14 in a Staphylococcus aureus-infected male rat model of full-thickness skin defect during the treatment of chronic wounds in Examples 1, 1, 2, 1, and 2 of the present invention.

[0041] Figure 11 This is a schematic diagram showing bacterial colonies taken from wound tissue and cultured on agar plates on days 3 and 7 during the treatment of chronic wounds in Embodiment 1, Comparative Example 1, Comparative Example 2, Control Group 1, and Control Group 2 of the present invention.

[0042] Figure 12 The graph shows the relative activity test results of bacteria around the wound tissue during the treatment of chronic wounds in Example 1, Comparative Example 1, Comparative Example 2, Control Group 1, and Control Group 2 of the present invention. Detailed Implementation

[0043] The present invention will be further described below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

[0044] The manufacturers and brands mentioned in the following embodiments are merely examples. The core of this invention lies in the technical solution itself, and it is not intended to limit specific manufacturers or brands. Products from other manufacturers and brands that meet the technical requirements and performance indicators specified in this invention can also meet the application requirements of this invention and are all feasible choices.

[0045] All water used in the following examples and comparative examples is deionized water.

[0046] The test methods for the relevant performance indicators in the following embodiments and comparative examples are as follows:

[0047] Inhibition rate: Sterile cotton swabs were used to sample wound tissue along the edge after 3 and 7 days. The bacterial end of the cotton swab was immersed in 2 mL of sterile PBS solution and sonicated in a water bath at 40 Hz and 25 ℃ for 5 min to elute the bacteria into the PBS solution. Then, 100 μL of the sonicated bacterial suspension was evenly spread on an agar plate and incubated in a constant temperature incubator at 37 ℃ and 65% relative humidity for 18-20 h. The number of colonies on the plate was recorded and the inhibition rate of the bacteria was calculated. The formula for calculating the inhibition rate is: Y = (W0 - Wt) / W0 × 100%, where Y is the inhibition rate of the sample, W0 is the number of colonies on the control group plate, and Wt is the number of colonies on the experimental group plate.

[0048] Wound healing rate: After the full-thickness skin wound defect modeling is completed, the wound surface dressing is removed and photographed at different time points (e.g., 1 day, 3 days, 7 days, 14 days). Then, the wound area is calculated using ImageJ software. The formula for calculating the healing rate is: Q=(S0-Sx) / S0×100%, where Q is the wound healing rate, S0 is the wound area before the magnetically controlled dual-effect intelligent dressing is applied, and Sx is the wound area after the magnetically controlled dual-effect intelligent dressing is applied at different time points.

[0049] Example 1

[0050] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0051] (1) Preparation of raw materials;

[0052] Weakly alkaline solution: composed of NH3·H2O, anhydrous ethanol and water, wherein the concentration of NH3·H2O is 0.267M, the concentration of anhydrous ethanol is 3.18M, ​​and the pH is 8.5;

[0053] Solution containing reducing compound and antibacterial metal salt: solute is reducing compound and antibacterial metal salt, solvent is water, reducing compound is dopamine, antibacterial metal salt is silver nitrate, concentration of reducing compound is 9 mM, concentration of antibacterial metal salt is 59 mM;

[0054] Oxidizing agent: Ferric nitrate nonahydrate;

[0055] water;

[0056] Solution containing conductive monomer: solute is conductive monomer, conductive monomer is pyrrole, solvent is cyclohexane, concentration is 0.36M;

[0057] (2) First, soak a 0.3 mm thick cotton gauze in a weakly alkaline solution at 20°C for 15 min. Then, add a solution containing reducing compounds and antibacterial metal salts to the solution. Stir the solution at 20°C in the dark for 1 h. Remove the solution and perform post-treatment to obtain an antibacterial fabric (such as...). Figure 1 As shown in the figure, the silver nanoparticles are encapsulated by polydopamine (PDA). The mass-to-volume ratio of cotton gauze, weak alkaline solution, solution containing reducing compound and antibacterial metal salt is 1.2 g: 37.6 mL: 5 mL.

[0058] The above-mentioned method utilizes dopamine to reduce silver ions on the fabric surface during oxidative polymerization. In this process, dopamine is converted into polydopamine, and silver ions are converted into silver nanoparticles. The generated polydopamine will coat the surface of the silver nanoparticles.

[0059] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing the oxidant, antibacterial fabric and water is obtained; wherein the concentration of the oxidant is 30.1 mM and the concentration of the antibacterial fabric is 1.2 g / 20 mL;

[0060] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 12 hours. A polypyrrole coating will be deposited on the surface of the antibacterial fabric, thus obtaining the magnetically controlled dual-effect intelligent dressing (e.g. Figure 2 As shown in the figure, a conductive coating is deposited on the surface of the antibacterial fabric. The volume ratio of the mixture containing the oxidant, the antibacterial fabric and water to the solution containing the conductive monomer is 1:1.

[0061] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0062] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0063] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0064] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0065] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0066] (b) The magnetically controlled dual-effect intelligent dressing prepared above was attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator was activated for 1 hour daily. The main purpose was to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator was activated for 0.25 hours daily. The main purpose was to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. (The current output of the magnetically controlled dual-effect intelligent dressing under the rotating magnetic field was tested using a digital source meter.) Figure 3 As shown in the figure, the magnetically controlled dual-effect intelligent dressing can generate an alternating current of about 20μA under a rotating magnetic field. Among them, the rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device has a rotation speed of 600rpm and a magnetic induction intensity of 90mT.

[0067] The above treatment methods for chronic wounds showed that the antibacterial rate was 77.94% at 3 days, 99.91% at 7 days, and 96.82% at 14 days.

[0068] Comparative Example 1

[0069] A method for preparing a magnetically controlled dual-effect intelligent dressing is the same as in Example 1.

[0070] A treatment device for chronic wounds, as in Example 1.

[0071] A treatment method for chronic wounds follows the same procedure as in Example 1, except that the rotating magnetic field generator is not activated after the magnetically controlled dual-effect intelligent dressing is applied to the chronic wound.

[0072] The above treatment methods for chronic wounds showed that the antibacterial rate was 57.99% on day 3, 94.41% on day 7, and 88.51% on day 14. The presence of a small amount of turbid exudate and slight local redness and swelling at the wound site indicated that the bacterial infection was suppressed to a certain extent, and the area and depth of the wound were gradually decreasing. However, a large wound area still existed on day 14.

[0073] Comparing Comparative Example 1 and Example 1, it can be seen that after the rotating magnetic field treatment of the magnetically controlled dual-effect intelligent dressing was removed, the pus at the wound site worsened, the bacterial infection became more severe, and the wound healing rate decreased. This is because the antibacterial effect of the dressing mainly depends on the release of silver ions in the magnetically controlled dual-effect intelligent dressing. Without the rotating magnetic field treatment, only a small amount of silver ions are slowly released into the wound. The trace amount of antibacterial agent cannot quickly kill the bacteria at the wound site. The bacteria multiply rapidly at the wound site, causing excessive and persistent inflammation. The wound cannot form healthy granulation tissue, which increases the burden of wound healing and hinders the transition of the wound to the proliferation and remodeling phase, ultimately leading to a decrease in the wound healing rate.

[0074] To verify the therapeutic effect of the chronic wound treatment device prepared using the magnetically controlled dual-effect intelligent dressing of the present invention on chronic wounds, the treatment results of Example 1, Comparative Example 1, Control Group 1, and Control Group 2 are compared as follows:

[0075] Control group 1: A treatment method for chronic wounds, basically the same as in Example 1, except that: no chronic wound treatment device is used, and only gauze is used to treat the chronic wounds.

[0076] Control group 2: A treatment method for chronic wounds, basically the same as in Example 1, except that the magnetically controlled dual-effect smart dressing in the chronic wound treatment device was replaced with gauze.

[0077] The temperatures during the 1-hour treatment of chronic wounds in Control Group 1, Control Group 2, Comparative Example 1, and Example 1 were compared. Figures 4 to 7 As shown, the magnetically controlled dual-effect smart dressing used in Example 1 exhibits a significant temperature rise under a rotating magnetic field, as observed from a microscopic perspective.

[0078] The cumulative release of silver ions during the treatment of chronic wounds in Comparative Example 1 and Example 1 was compared, and the results are as follows: Figure 8 As shown in the figure, the magnetically controlled dual-effect smart dressing releases more silver ions into the solution under magnetic field treatment. This is because the microscopic heat change of the conductive dressing promotes the release of silver ions.

[0079] Comparative Example 2

[0080] A method for preparing a magnetically controlled dual-effect intelligent dressing is the same as in Example 1.

[0081] A treatment device for chronic wounds, as in Example 1.

[0082] A treatment method for chronic wounds follows the same procedure as in Example 1, except that the rotating magnetic field generator is activated for 0.25 hours daily from day 1 to day 7.

[0083] Treatment of chronic wounds using the above methods showed that the antibacterial rate was 65.23% at 3 days, 96.52% at 7 days, and 91.15% at 14 days. The exudate at the wound site decreased, and the redness and swelling around the tissue improved, proving that the bacterial infection was under control. The area and depth of the wound decreased rapidly, and granulation tissue formed well. However, the wound still could not be completely healed by the 14th day.

[0084] Comparing Comparative Example 2 with Example 1, it can be seen that the antibacterial performance of this comparative example decreased and the wound healing rate was low during the treatment of chronic wounds. This is because the rotating magnetic field treatment time of the magnetically controlled dual-effect intelligent dressing in this comparative example was too short, from 1 to 7 days. Although the wound healing rate was slightly accelerated due to the regulation of cell proliferation and migration by electrical stimulation, the magnetically mediated thermal effect had no significant impact on the release of silver ions in a short period of time. Therefore, it was unable to quickly and thoroughly kill the bacteria in the wound when the bacterial infection at the wound site was more severe. The continuous bacterial infection would prolong the inflammatory period of the wound, impair cell behavior, and ultimately limit wound healing.

[0085] The wound healing effects of male rats with full-thickness skin defect models during the treatment of chronic wounds were compared using Example 1, Comparative Example 1, Comparative Example 2, Control Group 1, and Control Group 2. The results are as follows: Figure 9 , Figure 10 As shown, in the early stages of bacterial infection, the primary task is to rapidly release silver ions to exert effective antibacterial effects, prevent infection from worsening, and regulate the immune microenvironment, promoting the transition from the inflammatory phase to the proliferative phase. However, in the later stages of healing (proliferation and remodeling), the treatment goal shifts from enhancing antibacterial effects to promoting tissue regeneration and repair, which necessitates reducing Ag. + The release of [Ag] is to avoid potential cytotoxicity and impaired healing. Therefore, in Example 1, Ag is directly modulated by adjusting the duration of exposure to the rotating magnetic field. +The release and cellular response were coordinated with the dynamic needs of the wound healing process (i.e., the rotating magnetic field generator was activated for 1 hour daily from day 1 to 7, and for 0.25 hours daily from day 8 onwards). Representative photographs of the wound sites in the figures show that in the early stages (days 1-7), due to widespread bacterial proliferation, yellow pus formed in the wound bed in both control groups 1 and 2. By day 14, the wound healing rates in the two groups were only 76.92% and 77.64%, respectively, indicating that the antibacterial dressing had no positive effect on wound healing. In contrast, Comparative Example 1 showed reduced pus accumulation, indicating effective inhibition of bacterial infection and thus accelerated wound healing. However, its wound healing rate at 14 days was only 88.51%, demonstrating that the antibacterial, electroactive dressing can promote wound healing by reducing bacterial infection and enhancing intercellular signaling. In Comparative Example 2, due to the shorter daily activation time of the rotating magnetic field generator, its antibacterial and healing effects were also reduced compared to Example 1. Example 1 showed minimal pus formation, with the wound essentially closed within 14 days, achieving a healing rate as high as 96.82% in 14 days. Furthermore, new hair grew around the wound, demonstrating that the antibacterial and electroactive dressing, based on RMF regulation, effectively and rapidly reduced bacterial infection in the wound. The generated wireless microcurrent restored the wound's endogenous electric field microenvironment, significantly promoting wound healing.

[0086] Bacterial colonies were compared between samples taken from wound tissues of Examples 1, 1, 2, 1, and 2 during the treatment of chronic wounds and cultured on agar plates for 3 and 7 days. The results are as follows: Figure 10 , Figure 11 As shown, from Figure 11 The results show that control groups 1 and 2 had a higher number of bacterial colonies, indicating a more severe bacterial infection at the wound site; due to Ag + With continued release, the bacterial colonies in Comparative Example 1 gradually decreased; while in Example 1, the enhanced magnetocaloric effect of RMF promoted the growth of Ag. + The release of this substance results in the lowest number of bacterial colonies, effectively inhibiting bacterial infection at the wound site and hindering wound healing.

[0087] from Figure 12 As can be seen, bacteria in control groups 1 and 2 maintained high activity throughout, with inhibition rates of 32.08% and 27.10% respectively after 7 days. Comparative example 1, due to the slow and continuous release of silver ions, initially inhibited bacterial activity, achieving an inhibition rate of 94.41% after 7 days, but with a lower wound healing rate. In contrast, example 1 showed an inhibition rate as high as 99.91%, demonstrating that the magnetocaloric effect of the rotating magnetic field-enhanced dressing further promoted the inhibition of Ag... + The release of [something] enhances the antibacterial effect of the dressing;

[0088] The above results demonstrate that the antibacterial and electrostimulation properties of the magnetically controlled dual-effect smart dressing can be precisely controlled on demand by programmably adjusting the time of the rotating magnetic field. + The release of [something] allows treatment to synchronize with the wound healing phase, ultimately accelerating the healing process.

[0089] Example 2

[0090] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0091] (1) Preparation of raw materials;

[0092] Weakly alkaline solution: Tris buffer, pH=8.5;

[0093] Solution containing reducing compound and antibacterial metal salt: the solute is reducing compound and antibacterial metal salt, the solvent is water, the reducing compound is 5-hydroxytryptophan, the antibacterial metal salt is sodium chloroaurate, the concentration of the reducing compound is 10 mM, and the concentration of the antibacterial metal salt is 89 mM.

[0094] Oxidizing agent: Ferric chloride hexahydrate;

[0095] water;

[0096] Solution containing conductive monomer: solute is conductive monomer, conductive monomer is aniline, solvent is water, concentration is 0.54M;

[0097] (2) First, add cotton gauze with a thickness of 0.33 mm into a weak alkaline solution and soak it at 21°C for 20 min. Then, add a solution containing reducing compounds and antibacterial metal salts to it. Stir it in the dark at 21°C for 1.5 h. Take it out and perform post-treatment to obtain antibacterial fabric. The mass-volume ratio of cotton gauze, weak alkaline solution, and solution containing reducing compounds and antibacterial metal salts is 1.44 g: 45.12 mL: 6 mL.

[0098] The above-mentioned method utilizes 5-hydroxytryptophan to reduce gold ions on the fabric surface during oxidative polymerization. In this process, 5-hydroxytryptophan is transformed into poly(5-hydroxytryptophan), and gold ions are transformed into gold nanoparticles. The generated poly(5-hydroxytryptophan) will coat the surface of the gold nanoparticles.

[0099] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing the oxidant, antibacterial fabric and water is obtained; wherein the concentration of the oxidant is 45.15mM and the concentration of the antibacterial fabric is 1.4g / 20mL;

[0100] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 14 hours. A polyaniline coating will be deposited on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect intelligent dressing. The volume ratio of the mixture containing oxidant, antibacterial fabric and water to the solution containing conductive monomer is 1:1.2.

[0101] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0102] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0103] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0104] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0105] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0106] (b) The magnetically controlled dual-effect intelligent dressing prepared above is attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator is activated for 1 hour daily. The main purpose is to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator is activated for 0.25 hours daily. The main purpose is to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. The rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device rotates at 350 rpm, with a magnetic induction intensity of 91 mT.

[0107] The above treatment methods for chronic wounds showed that the antibacterial rate was 67.23% after 3 days, 95.01% after 7 days, and 90.53% after 14 days.

[0108] Example 3

[0109] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0110] (1) Preparation of raw materials;

[0111] Weakly alkaline solution: carbonate buffer, pH=8.5;

[0112] Solution containing reducing compound and antibacterial metal salt: the solute is reducing compound and antibacterial metal salt, the solvent is water, the reducing compound is tannic acid, the antibacterial metal salt is silver acetate, the concentration of the reducing compound is 15 mM, and the concentration of the antibacterial metal salt is 118 mM.

[0113] Oxidizing agent: anhydrous ferric chloride;

[0114] water;

[0115] Solution containing conductive monomer: The solute is a conductive monomer, the conductive monomer is 3,4-ethylenedioxythiophene monomer, the solvent is water, and the concentration is 0.72M;

[0116] (2) First, add cotton gauze with a thickness of 0.38 mm to a weak alkaline solution and soak it at 23°C for 30 min. Then, add a solution containing reducing compounds and antibacterial metal salts to it. Stir it at 23°C in the dark for 2 h. Then, take it out and perform post-treatment to obtain antibacterial fabric. The mass-volume ratio of cotton gauze, weak alkaline solution, and solution containing reducing compounds and antibacterial metal salts is 1.68 g: 52.738 mL: 7 mL.

[0117] The above-mentioned method utilizes tannic acid to reduce silver ions on the fabric surface during oxidative polymerization. Tannic acid is converted into polytannic acid, and silver ions are converted into silver nanoparticles. The generated polytannic acid will coat the surface of the silver nanoparticles.

[0118] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing the oxidant, antibacterial fabric and water is obtained; wherein the concentration of the oxidant is 60.2 mM and the concentration of the antibacterial fabric is 1.6 g / 20 mL;

[0119] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 16 hours. A poly(3,4-ethylenedioxythiophene) coating will be deposited on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect smart dressing. The volume ratio of the mixture containing oxidant, antibacterial fabric and water to the solution containing conductive monomer is 1:1.4.

[0120] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0121] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0122] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0123] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0124] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0125] (b) The magnetically controlled dual-effect intelligent dressing prepared above is attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator is activated for 1 hour daily. The main purpose is to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator is activated for 0.25 hours daily. The main purpose is to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. The rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device rotates at 400 rpm, with a magnetic induction intensity of 93 mT.

[0126] The above treatment methods for chronic wounds showed that the antibacterial rate was 68.91% after 3 days, 96.81% after 7 days, and 91.52% after 14 days.

[0127] Example 4

[0128] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0129] (1) Preparation of raw materials;

[0130] Weakly alkaline solution: borate buffer solution, pH=8.5;

[0131] Solution containing reducing compound and antibacterial metal salt: the solute is reducing compound and antibacterial metal salt, the solvent is water, the reducing compound is dopamine, the antibacterial metal salt is silver nitrate, the concentration of the reducing compound is 18 mM, and the concentration of the antibacterial metal salt is 177 mM.

[0132] Oxidizing agent: Ammonium persulfate;

[0133] water;

[0134] Solution containing conductive monomer: The solute is a conductive monomer, the conductive monomer is triphenylamine, the solvent is water, and the concentration is 1.08M;

[0135] (2) First, add cotton gauze with a thickness of 0.4 mm to a weak alkaline solution and soak it at 25°C for 40 min. Then, add a solution containing reducing compounds and antibacterial metal salts to it. Stir it at 25°C in the dark for 3 h. After that, take it out and perform post-treatment to obtain antibacterial fabric. The mass-volume ratio of cotton gauze, weak alkaline solution, and solution containing reducing compounds and antibacterial metal salts is 1.8 g: 56.4 mL: 7.5 mL.

[0136] The above-mentioned method utilizes dopamine to reduce silver ions on the fabric surface during oxidative polymerization. In this process, dopamine is converted into polydopamine, and silver ions are converted into silver nanoparticles. The generated polydopamine will coat the surface of the silver nanoparticles.

[0137] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing the oxidant, antibacterial fabric and water is obtained; wherein the concentration of the oxidant is 75.25mM and the concentration of the antibacterial fabric is 1.8g / 20mL;

[0138] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 18 hours. A polytriphenylamine coating will be deposited on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect intelligent dressing. The volume ratio of the mixture containing oxidant, antibacterial fabric and water to the solution containing conductive monomer is 1:1.5.

[0139] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0140] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0141] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0142] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0143] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0144] (b) The magnetically controlled dual-effect intelligent dressing prepared above is attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator is activated for 1 hour daily. The main purpose is to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator is activated for 0.25 hours daily. The main purpose is to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. The rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device rotates at 450 rpm, with a magnetic induction intensity of 95 mT.

[0145] The above treatment methods for chronic wounds showed that the antibacterial rate was 69.35% after 3 days, 97.47% after 7 days, and the wound healing rate was 92.63% after 14 days.

[0146] Example 5

[0147] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0148] (1) Preparation of raw materials;

[0149] Weakly alkaline solution: phosphate buffer, pH=8.5;

[0150] Solution containing reducing compound and antibacterial metal salt: the solute is reducing compound and antibacterial metal salt, the solvent is water, the reducing compound is 5-hydroxytryptophan, the antibacterial metal salt is silver nitrate, the concentration of the reducing compound is 27 mM, and the concentration of the antibacterial metal salt is 207 mM;

[0151] Oxidizing agent: Potassium persulfate;

[0152] water;

[0153] Solution containing conductive monomer: solute is conductive monomer, conductive monomer is indole, solvent is water, concentration is 1.26M;

[0154] (2) First, add cotton gauze with a thickness of 0.45 mm into a weak alkaline solution and soak it at 27°C for 50 min. Then, add a solution containing reducing compounds and antibacterial metal salts to it. Stir it at 27°C in the dark for 4 h. Take it out and perform post-treatment to obtain antibacterial fabric. The mass-volume ratio of cotton gauze, weak alkaline solution, and solution containing reducing compounds and antibacterial metal salts is 2.16 g: 67.806 mL: 9 mL.

[0155] The above-mentioned method utilizes 5-hydroxytryptophan to reduce silver ions on the fabric surface during oxidative polymerization. In this process, 5-hydroxytryptophan is transformed into poly(5-hydroxytryptophan), and silver ions are transformed into silver nanoparticles. The generated poly(5-hydroxytryptophan) will coat the surface of the silver nanoparticles.

[0156] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing the oxidant, antibacterial fabric and water is obtained; wherein the concentration of the oxidant is 90.3 mM and the concentration of the antibacterial fabric is 2 g / 20 mL.

[0157] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 20 hours. A polyindole coating will be deposited on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect smart dressing. The volume ratio of the mixture containing oxidant, antibacterial fabric and water to the solution containing conductive monomer is 1:1.8.

[0158] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0159] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0160] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0161] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0162] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0163] (b) The magnetically controlled dual-effect intelligent dressing prepared above is attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator is activated for 1 hour daily. The main purpose is to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator is activated for 0.25 hours daily. The main purpose is to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. The rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device rotates at 500 rpm, with a magnetic induction intensity of 97 mT.

[0164] The above treatment methods for chronic wounds showed that the antibacterial rate was 70.25% after 3 days, 97.63% after 7 days, and 94.26% after 14 days.

[0165] Example 6

[0166] A method for preparing a magnetically controlled dual-effect smart dressing, comprising the following steps:

[0167] (1) Preparation of raw materials;

[0168] Weakly alkaline solution: glycine buffer, pH=8.5;

[0169] Solution containing reducing compound and antibacterial metal salt: the solute is reducing compound and antibacterial metal salt, the solvent is water, the reducing compound is dopamine, the antibacterial metal salt is chloroauric acid, the concentration of the reducing compound is 36 mM, and the concentration of the antibacterial metal salt is 236 mM.

[0170] Oxidizing agent: Sodium periodate;

[0171] water;

[0172] Solution containing conductive monomer: solute is conductive monomer, conductive monomer is carbazole, solvent is water, concentration is 1.44M;

[0173] (2) First, add cotton gauze with a thickness of 0.5 mm into a weak alkaline solution and soak it at 30°C for 60 min. Then, add a solution containing reducing compounds and antibacterial metal salts to it. Stir it at 30°C in the dark for 5 h. Take it out and perform post-treatment to obtain antibacterial fabric. The mass-volume ratio of cotton gauze, weak alkaline solution, and solution containing reducing compounds and antibacterial metal salts is 2.4 g: 75.34 mL: 10 mL.

[0174] The above process involves the reduction of gold ions during the oxidation and polymerization of dopamine on the fabric surface. In this process, dopamine is converted into polydopamine, and gold ions are converted into gold nanoparticles. The generated polydopamine will coat the surface of the gold nanoparticles.

[0175] (3) After mixing the oxidant, antibacterial fabric and water evenly, a mixture containing oxidant, antibacterial fabric and water is obtained; wherein, the concentration of oxidant is 120.4 mM and the concentration of antibacterial fabric is 2.4 g / 20 mL;

[0176] (4) First, freeze the mixture containing oxidant, antibacterial fabric and water at -20°C until solidified. Then, drop the solution containing conductive monomer onto the ice surface and react at 4°C for 24 hours. A polycarbazole coating will be deposited on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect intelligent dressing. The volume ratio of the mixture containing oxidant, antibacterial fabric and water to the solution containing conductive monomer is 1:2.

[0177] A treatment device for chronic wounds includes a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing prepared above.

[0178] A rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

[0179] The rotating magnetic field generator includes a motor and a neodymium iron boron permanent magnet, which is fixed to the output shaft of the motor.

[0180] A treatment method for chronic wounds, using the aforementioned chronic wound treatment device, the specific process of which is as follows:

[0181] (a) A full-thickness skin defect model with a diameter of 10 mm was created in a male rat (300 g, provided by Spifor (Beijing) Biotechnology Co., Ltd.), and then 100 μL of Staphylococcus aureus bacterial suspension (Staphylococcus aureus concentration of 1 × 10⁻⁶) was dripped into the wound. 6 After soaking the wound with CFU / mL for 30 minutes, the wound was then bandaged with medical gauze and tape. A chronic wound model with bacterial infection was obtained after 24 hours.

[0182] (b) The magnetically controlled dual-effect intelligent dressing prepared above is attached and fixed onto the chronic wound model. From day 1 to day 7, the rotating magnetic field generator is activated for 1 hour daily. The main purpose is to promote the release of antibacterial metal ions, achieve rapid bacterial kill, and reduce infection. From day 8 onwards, the rotating magnetic field generator is activated for 0.25 hours daily. The main purpose is to generate microcurrents to regulate the proliferation and differentiation of fibroblasts, macrophages, endothelial cells, nerve cells, etc., promoting rapid wound healing and functional reconstruction. The rotating magnetic field generated by the rotating magnetic field generator in the chronic wound treatment device rotates at 300 rpm, with a magnetic induction intensity of 100 mT.

[0183] The above treatment methods for chronic wounds showed that the antibacterial rate was 69.72% after 3 days, 95.62% after 7 days, and 93.91% after 14 days.

Claims

1. A method for preparing a magnetically controlled dual-effect smart dressing, characterized in that, The method comprises the following steps: reducing antibacterial metal ions on the surface of the fabric by using a reducing compound to obtain an antibacterial fabric, and depositing a conductive coating on the surface of the antibacterial fabric to obtain the magnetically controlled dual-effect intelligent dressing.

2. The method for preparing a magnetic controlled dual-purpose intelligent dressing according to claim 1, characterized in that, The fabric is cotton gauze, the thickness of the fabric is 0.3-0.5mm, the reducing compound is dopamine, 5-hydroxytryptophan or tannic acid, and the antibacterial metal ion is silver ion or gold ion.

3. The method for preparing a magnetically controlled dual-effect intelligent dressing according to claim 2, characterized in that, The process of reducing the antibacterial metal ions on the surface of the fabric by using the reducing compound comprises the following steps: first, immersing the fabric in a weak alkaline solution for 15-60min, then adding a solution containing the reducing compound and the antibacterial metal salt, stirring in the dark for 1-5h, and then taking out and performing post-processing.

4. The method for preparing a magnetically controlled dual-effect intelligent dressing according to claim 3, characterized in that, The concentration of the reducing compound in the solution containing the reducing compound and the antibacterial metal salt is 9-36mM, and the concentration of the antibacterial metal salt is 59-236mM; the mass-volume ratio of the fabric, the weak alkaline solution and the solution containing the reducing compound and the antibacterial metal salt is 1.2-2.4g:37.67-75.34mL:5-10mL.

5. The method for preparing a magnetically controlled dual-effect intelligent dressing according to claim 1, characterized in that, The conductive coating is a polypyrrole coating, a PEDOT coating, a polytriphenylamine coating, a polycarbazole coating or a polyaniline coating.

6. The method of claim 5, wherein the magnetorheological dual-acting smart dressing is prepared by the steps of: The process of depositing the conductive coating on the surface of the antibacterial fabric comprises the following steps: first, freezing a mixture containing an oxidizing agent, the antibacterial fabric and water to solidification, and then dropping a solution containing a conductive monomer on the ice surface and reacting at 4℃ for 12-24h.

7. The method for preparing a magnetically controlled dual-effect intelligent dressing according to claim 6, characterized in that, The oxidizing agent is ferric nitrate nonahydrate, ferric chloride hexahydrate, anhydrous ferric chloride, potassium persulfate or ammonium persulfate; in the mixture, the concentration of the oxidizing agent is 30.1-120.4mM, and the concentration of the antibacterial fabric is 1.2-2.4g / 20mL; the concentration of the solution containing the conductive monomer is 0.36-1.44M; and the volume ratio of the mixture to the solution containing the conductive monomer is 1:1-2.

8. A magnetron dual-effect smart dressing characterized in that, The magnetically controlled dual-effect intelligent dressing is prepared by using the method of any one of claims 1-7.

9. A device for the treatment of chronic wounds, characterized in that The magnetically controlled dual-effect intelligent dressing comprises a rotating magnetic field generator and the magnetically controlled dual-effect intelligent dressing of claim 8; the rotating magnetic field generator is used to generate a rotating magnetic field, and the magnetically controlled dual-effect intelligent dressing is located in the rotating magnetic field.

10. A device for the treatment of chronic wounds according to claim 9, characterised in that The rotating magnetic field generator comprises a motor and a cobalt-iron-boron permanent magnet, and the cobalt-iron-boron permanent magnet is fixed on the output shaft of the motor; the rotating speed of the rotating magnetic field is 300-600rpm, and the magnetic induction intensity is 90-100mT.

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