Device for detecting organic selenium in plant sample

Abstract

The invention relates to the technical field of plant optical detection, and particularly discloses a plant sample organic selenium detection device which comprises a detection table, a detection box is fixedly connected to one side of the top end of the detection table, a detection module is arranged on one side of the detection box, and the detection module comprises a fluorescence sensing subunit and a mass spectrum verification subunit. According to the eccentric transmission design, motor power is converted into longitudinal driving force, multi-dimensional vibration is formed by the longitudinal driving force and transverse swing, inertia thrust is generated on a solution from different directions, flowing is more uniform and smooth, the problem that the flow speed is not uniform under traditional single driving is solved, the multi-dimensional vibration destroys the impurity attachment stability, residual deposition is reduced, and the service life of the solution is prolonged. The first push column mechanism is matched with the first push column mechanism to form an omnibearing power-assisted system, so that the core problems of low solution transmission efficiency and more residues under a single driving mode in the background technology are thoroughly solved, and the plant sample detection effect is improved.

Description

Technical Field

[0001] This invention relates to the field of plant optical detection technology, and in particular to a device for detecting organic selenium in plant samples. Background Technology

[0002] Plant samples refer to various plant tissues used for testing, such as roots, stems, leaves, or fruits of selenium-enriched tea, sweet potatoes, and grains. Organic selenium is the form of selenium after absorption and transformation by plants, and its bioavailability is much higher than that of inorganic selenium. It is crucial for human antioxidant and immune enhancement. However, the forms of selenium in plants are complex, and the total selenium content cannot reflect the effective nutritional value. Furthermore, excessive intake poses a risk. Therefore, it is necessary to specifically test organic selenium to clarify its true content and form, and to provide a basis for the quality grading of selenium-enriched agricultural products.

[0003] For example, Chinese patent CN213749578U discloses an optical detection device for plant leaf images, including: a base, a light-transmitting aperture, an optical detection element, a housing, a light source assembly, and a control processor, wherein the control processor is connected to the optical detection element and the light source assembly respectively. This device is convenient for operators to carry and operate, enabling real-time detection of plant leaf samples. However, after the plant solution is processed, it needs to enter a chromatographic column for separation, followed by qualitative and quantitative analysis in the detection unit. However, in existing chromatographic columns, impurities such as plant fibers and polysaccharides easily adhere to the inner wall and gaps in the packing material during the guiding and transporting of the plant solution, leading to residual sample deposition that contaminates subsequent plant solutions, causing cross-interference. Furthermore, the effective flow space within the column is reduced, resulting in longer sample retention times and significantly increasing the detection cycle, thus hindering plant sample detection and reducing detection efficiency. Summary of the Invention

[0004] The purpose of this invention is to provide a device for detecting organic selenium in plant samples, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides a device for detecting organic selenium in plant samples, comprising a detection stage, a detection box fixedly connected to one side of the top of the detection stage, a detection module provided on one side of the detection box, the detection module including a fluorescence sensing subunit and a mass spectrometry verification subunit, both of which are mounted on the detection box, a rubber liquid guiding sleeve fixedly connected to the top of the detection box, a chromatographic column provided on the upper side of the rubber liquid guiding sleeve, the chromatographic column separating organic selenium from inorganic selenium, an assembly block fixedly connected to one side of the chromatographic column, a fixing frame fixedly connected to one side of the detection stage on the side of the detection box, a first mounting frame fixedly connected to one side of the fixing frame, a first column pushing mechanism provided on one side of the first mounting frame, a second mounting frame movably connected to one side of the first mounting frame via the first column pushing mechanism, a second column pushing mechanism provided on one side of the second mounting frame, one side of the second column pushing mechanism connected to one side of the chromatographic column, a fixing mechanism provided between the second column pushing mechanism and the chromatographic column, and a connecting component provided between the rubber liquid guiding sleeve and the chromatographic column. The fluorescence sensing subunit consists of a quartz detection cell, a near-infrared excitation source, a specific fluorescent probe array, and a fluorescence detector. The quartz detection cell is fixedly connected inside the detection box, with its inlet sealed to the outlet of a rubber liquid-conducting sleeve. The outlet is connected to the mass spectrometry verification subunit via a pipeline. The near-infrared excitation source and the fluorescence detector are respectively located on both sides of the quartz detection cell. The fluorescent probe array is fixed to the inner wall of the quartz detection cell and includes Se-N bond structure probes, selenoazole probes, and Ebselen-modified probes. After specifically binding to the target organic selenium molecules in the plant solution flowing down the chromatographic column, it generates a characteristic fluorescence signal under the illumination of the near-infrared excitation source. The fluorescence detector captures the fluorescence signal and converts it into an electrical signal, which is then transmitted to the central control module. The wavelength of the near-infrared excitation source is 785 nm. The fluorescence detector is a high-sensitivity photomultiplier tube with a detection range of 650-900 nm and is equipped with a temperature control module to maintain a constant detection environment temperature of 37°C. Because organic selenium has a weaker interaction with the packing material, it first flows out of the column with the mobile phase and enters the fluorescence sensing subunit of the detection cartridge and the subsequent mass spectrometry verification subunit through the rubber liquid guide sleeve to complete the detection. Inorganic selenium has a stronger interaction with the packing material and a longer retention time, so it will lag behind organic selenium in its movement within the column. After organic selenium has completely flowed out, it can be continuously washed by the subsequent mobile phase.

[0006] Furthermore, the first pusher mechanism includes a first motor, which is fixedly connected to a first mounting bracket. A drive gear is fixedly connected to the output end of the first motor. A driven gear is meshed with one side of the drive gear. A first transmission rod is fixedly connected to one side of the driven gear. A cam block is fixedly connected to one side of the first transmission rod. A first transmission frame is provided on the outer side of the cam block. A first guide assembly is provided on one side of the mounting bracket. The first transmission frame is slidably connected to the mounting bracket through the first guide assembly. One side of the first transmission frame is fixedly connected to one side of the second mounting bracket. A first force-receiving groove is provided inside the first transmission frame. One side of the cam block is in movable contact with the left and right inner walls of the first force-receiving groove.

[0007] Furthermore, the first guide assembly includes support rods, which are fixedly connected to both sides of the top of the fixed frame. One guide rod is slidably connected to one side of each of the two support rods. The first guide rod is fixedly connected to the left and right outer walls of the first transmission frame. A guide sleeve is fixedly connected to one side of each support rod, and one side of the first guide rod slides through the guide sleeve.

[0008] Furthermore, the second pusher mechanism includes a second motor, which is fixedly connected inside the second mounting frame. A rotating disk is fixedly connected to the output end of the second motor. A second transmission rod is fixedly connected to one side of the rotating disk. A second transmission frame is provided on the outer side of the second transmission rod. A second guide assembly is provided on the side of the second mounting frame near the second transmission frame. The second transmission frame is slidably connected to one side of the second mounting frame through the second guide assembly. A connecting frame is fixedly connected to one side of the second guide assembly. The fixing mechanism is located on one side of the connecting frame. A second force groove is opened inside the second transmission frame. The second transmission rod is slidably connected inside the second force groove.

[0009] Furthermore, the second guide assembly includes a limiting block, which is fixedly connected to both sides of one end of the second mounting frame. A second guide rod is fixedly connected between the two limiting blocks. A slide block is slidably connected to the outer surface of the second guide rod. One end of the slide block is fixedly connected to one end of the second transmission frame, and the other end of the slide block is fixedly connected to one end of the connecting frame.

[0010] Furthermore, the fixing mechanism includes a first slot, which is located on one side of the connecting frame. A first insert block is inserted into the first slot, and one side of the first insert block is fixedly connected to one side of the assembly block. A second slot is provided on both sides of the assembly block, and a second insert block is inserted into the second slot. A driving assembly is provided on one side of the connecting frame, and the second insert block is fixedly connected to the second slot through the driving assembly. The driving assembly includes a fixing plate, which is fixedly connected to the side of the connecting frame away from the assembly block. A third motor is fixedly connected to one side of the fixing plate, and a bidirectional lead screw is fixedly connected to the output end of the third motor. The bidirectional lead screw is rotatably connected inside the fixing plate, and movable blocks are threaded to both sides of the bidirectional lead screw. One side of the movable block is slidably connected inside the fixing plate, and the second insert block is fixedly connected to the movable block.

[0011] Furthermore, the connecting assembly includes a first docking block, which is fixedly connected to the top end of the rubber liquid guiding sleeve, and a second docking block is fixedly connected to the bottom end of the chromatographic column. A stud is fixedly connected to the top end of the first docking block, and the stud is threadedly connected to the inside of the second docking block.

[0012] Compared with the prior art, the beneficial effects of the present invention are: Firstly, in this invention, by setting up a first column-pushing mechanism, during operation, a first motor drives the driving gear to rotate, which in turn drives the meshing driven gear to rotate the cam block via the first transmission rod. The cam block reciprocates within the force-receiving groove of the first transmission frame, causing the transmission frame to swing laterally along the guide assembly, thus driving the second mounting bracket and the chromatographic column. Gear transmission ensures stable and precise power transmission, while the cam structure converts rotational motion into smooth linear motion, providing lateral auxiliary power to the chromatographic column. The inertial force generated by the lateral swing assists the flow of the plant solution, reducing the adhesion and deposition of impurities such as fibers and polysaccharides on the column wall and between the packing materials, shortening the solution retention time, significantly improving the sample transfer efficiency to the detection module, and avoiding cross-interference caused by residual contamination of subsequent samples.

[0013] Secondly, in this invention, by setting a second column-pushing mechanism, a second motor drives a rotating disk to rotate, and a second transmission rod on the disk slides in the transmission groove of the second transmission frame, pushing the transmission frame along the guide assembly to drive the chromatographic column to swing longitudinally. The eccentric transmission design converts the motor power into longitudinal driving force, which, together with the lateral swing, forms multi-dimensional vibration, generating inertial thrust on the solution from different directions, making the flow more uniform and smooth, solving the problem of uneven flow rate under traditional single drive. Multi-dimensional vibration destroys the stability of impurity adhesion, reduces residual deposition, and, together with the first column-pushing mechanism, forms a comprehensive power assistance system, completely solving the core problems of low solution transmission efficiency and high residue under the single drive method in the background technology, and improving the detection effect of plant samples.

[0014] Thirdly, in this invention, a rubber liquid guiding sleeve is used to adapt to the operation of the dual-push column mechanism. The rubber liquid guiding sleeve has excellent flexibility and can adapt to deformation with the multi-dimensional swing of the chromatographic column, maintaining a sealed connection to avoid sample leakage and solving the problem of easy breakage of traditional rigid pipelines. The fixing mechanism fixes the chromatographic column through a double plug-in structure. The third motor drives the bidirectional lead screw to realize the automatic insertion and removal of the plug, quickly completing the assembly and disassembly. The connecting component uses a stud and an anti-rotation column to achieve a sealed connection, preventing the threads from loosening during swing, facilitating the maintenance, cleaning and specification replacement of the chromatographic column, and improving the versatility of the device. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the first pusher mechanism in this invention; Figure 3 In this invention Figure 2 A magnified structural diagram at point A; Figure 4 This is a schematic diagram of the detection box structure in this invention; Figure 5 This is a schematic diagram of the second mounting bracket structure in this invention; Figure 6 This is a schematic diagram of the fixing mechanism structure in this invention; Figure 7 In this invention Figure 5 A magnified structural diagram at point B; Figure 8 This is a schematic diagram of the structure of the chromatographic column from below in this invention.

[0016] In the diagram: 1. Detection stage; 2. Fixture; 3. Detection box; 4. Fluorescence sensing subunit; 5. Mass spectrometry verification subunit; 6. Rubber liquid guiding sleeve; 7. First mounting frame; 8. First column pushing mechanism; 81. First motor; 82. Driving gear; 83. Driven gear; 84. First transmission rod; 85. Cam block; 86. First transmission frame; 861. First force groove; 87. First guide assembly; 871. First guide rod; 872. Support rod; 873. Guide sleeve; 9. Second mounting frame; 10. Chromatographic column; 101. Assembly block; 11. Second column pushing mechanism; 111. Second motor; 112. Rotary... Turntable; 113, Second transmission rod; 114, Second transmission frame; 1141, Second force-receiving groove; 115, Second guide assembly; 1151, Limiting block; 1152, Second guide rod; 1153, Slide; 1154, Connecting frame; 12, Fixing mechanism; 121, First slot; 122, First insertion block; 123, Second slot; 124, Second insertion block; 125, Drive assembly; 1251, Fixing plate; 1252, Third motor; 1253, Bidirectional lead screw; 1254, Moving block; 13, Connecting assembly; 131, First docking block; 132, Second docking block; 133, Stud. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Please see Figures 1-8In this embodiment of the invention, a plant sample organic selenium detection device includes a detection platform 1. A detection box 3 is fixedly connected to one side of the top of the detection platform 1. A detection module is provided on one side of the detection box 3. The detection module includes a fluorescence sensing subunit 4 and a mass spectrometry verification subunit 5. Both the fluorescence sensing subunit 4 and the mass spectrometry verification subunit 5 are mounted on the detection box 3. A rubber liquid guiding sleeve 6 is fixedly connected to the top of the detection box 3. A chromatographic column 10 is provided on the upper side of the rubber liquid guiding sleeve 6. The chromatographic column 10 separates organic selenium from inorganic selenium. An assembly block 101 is fixedly connected to one side of the chromatographic column 10. A fixing frame 2 is fixedly connected to one side of the detection platform 1 on the side of the detection box 3. A first mounting frame 7 is fixedly connected to one side of the fixing frame 2. A first column pushing mechanism 8 is provided on one side of the first mounting frame 7. A second mounting bracket 9 is movably connected to one side of the mounting bracket 7 via a first pusher mechanism 8. A second pusher mechanism 11 is provided on one side of the second mounting bracket 9. One side of the second pusher mechanism 11 is connected to one side of the chromatographic column 10. A fixing mechanism 12 is provided between the second pusher mechanism 11 and the chromatographic column 10. A connecting component 13 is provided between the rubber liquid guiding sleeve 6 and the chromatographic column 10. By setting the first pusher mechanism 8 and the second pusher mechanism 11, the chromatographic column 10 can be adjusted in multiple dimensions. The rubber liquid guiding sleeve 6 is connected to the bottom of the chromatographic column 10 via the connecting component 13. The rubber liquid guiding sleeve 6 is connected to the detection box 3. The flexibility of the rubber liquid guiding sleeve 6 can adapt to the movement requirements of the chromatographic column 10, avoiding leakage during sample transmission. At the same time, the auxiliary vibration brought by the dual pusher mechanism can reduce the residue of the sample in the chromatographic column 10 and improve the transmission efficiency.

[0019] The fluorescence sensing subunit 4, serving as the detection head, is fixed in the upper half of the detection box 3. Its core component, the quartz detection cell, is horizontally embedded in a dedicated holder on the inner wall of the detection box 3. The inlet of the quartz detection cell faces vertically upward and is precisely connected to the outlet of the rubber liquid guide sleeve 6 at the top of the detection box 3 via a sealed connector, ensuring that the sample flowing down from the chromatographic column 10 can be directly injected into the detection cell. The near-infrared excitation light source and the fluorescence detector are symmetrically installed on the left and right sides of the quartz detection cell, with their central axes intersecting at a 135° angle to the central axis of the quartz detection cell. Inside the detection cell, the lenses of the light source and detector are isolated from the cell through quartz windows to avoid direct contact with the sample. The specific fluorescent probe array is uniformly fixed to the inner wall surface of the quartz detection cell by covalent bonds, completely covering the sample flow area. The mass spectrometry verification subunit 5 is located in the lower half of the detection box 3, directly below the fluorescence sensing subunit 4. The inlet of its electrospray ionization source is sealed to the outlet of the quartz detection cell through a polytetrafluoroethylene (PTFE) tube resistant to organic solvents. The tube is laid along the wall inside the detection box 3 and fixed with tube clamps. To ensure a stable sample transport path, the vacuum system is positioned close to the quadrupole electrostatic track trap mass analyzer in the mass spectrometry verification subunit 5. It connects to the ion chamber of the mass analyzer via a sealed interface, providing a high-vacuum detection environment. A nitrogen purging device is installed to the side of the electrospray ionization source, with its purging nozzle angled towards the spray needle of the ionization source, forming a gas flow barrier at a stable flow rate of 5 L / min to prevent external impurities from interfering with the ionization process. All components within the detection box 3 are fixed by a shielding bracket. The signal cables from the fluorescence sensing subunit 4 and the mass spectrometry verification subunit 5 converge and connect to the central control module through a sealed hole on the rear side of the detection box 3. The near-infrared excitation source of the fluorescence sensing subunit 4 is equipped with an independent temperature control module, integrated inside the side wall of the detection box 3. This module is attached to the source housing via a heat-conducting sheet, maintaining a stable detection environment temperature of 37°C. The mass analyzer in the mass spectrometry verification subunit 5 uses a built-in temperature control chip to regulate its own temperature, ensuring a stable resolution above 140,000 FWHM, meeting the requirements for accurate organic selenium detection. The mass spectrometry verification subunit 5 consists of an electrospray ionization source, a quadrupole electrostatic field orbital trap mass analyzer, a vacuum system, and a gas purging device. The inlet of the electrospray ionization source is connected to the outlet pipeline of the quartz detection cell of the fluorescence sensing subunit 4. The gas purging device is a nitrogen purging structure with a flow rate controlled at 5 L / min. The mass analyzer has a resolution of not less than 140000 FWHM and supports positive and negative ion switching modes. The detection data from the fluorescence sensing subunit 4 and the mass spectrometry verification subunit 5 are transmitted to the central control module via a shielded data cable. The central control module has a built-in organic selenium characteristic spectral database and mass spectrometry library. It uses a multivariate regression algorithm to fuse the dual-sensor data to achieve qualitative and quantitative analysis of organic selenium. The plant solution detected by the fluorescence sensing subunit 4 flows into the mass spectrometry verification subunit 5. The organic selenium molecules are ionized into charged ions by an electrospray ionization source. The quadrupole electrostatic field orbital trap mass analyzer accurately detects the mass-to-charge ratio of the charged ions and matches the characteristic mass-to-charge ratio data of organic selenium to achieve organic selenium speciation verification.

[0020] Please see Figures 1-3 The first pusher mechanism 8 includes a first motor 81, which is fixedly connected to a first mounting bracket 7. A drive gear 82 is fixedly connected to the output end of the first motor 81. A driven gear 83 is meshed with one side of the drive gear 82. A first transmission rod 84 is fixedly connected to one side of the driven gear 83. A cam block 85 is fixedly connected to one side of the first transmission rod 84. A first transmission frame 86 is provided on the outer side of the cam block 85. A first guide assembly 87 is provided on one side of the mounting bracket 2. The first transmission frame 86 is slidably connected to the mounting bracket 2 via the first guide assembly 87. One side of the first transmission frame 86 is fixedly connected to one side of a second mounting bracket 9. A first force-receiving groove 861 is provided inside the first transmission frame 86. The cam block 85... One side of the column is in active contact with the left and right inner walls of the first force groove 861. By setting the first push column mechanism 8, after the motor starts, it drives the active gear 82 to rotate. The active gear 82 drives the meshing driven gear 83 to rotate. The driven gear 83 drives the cam block 85 to rotate synchronously through the first transmission rod 84. The cam block 85 reciprocates against the groove wall in the first force groove 861 of the first transmission frame 86, so that the first transmission frame 86 moves horizontally along the first guide component 87, thereby driving the second mounting bracket 9 and the chromatographic column 10 to swing laterally. The cam structure can convert the rotational motion into a smooth linear reciprocating motion, providing lateral auxiliary power for the chromatographic column 10, reducing the adhesion of the sample in the column. At the same time, the swing frequency can be controlled by adjusting the speed of the first motor 81 to adapt to plant solutions of different viscosities.

[0021] Please see Figure 2The first guide assembly 87 includes support rods 872, which are fixedly connected to both sides of the top of the fixing frame 2. A first guide rod 871 is slidably connected to one side of each of the two support rods 872. The first guide rods 871 are fixedly connected to the left and right outer walls of the first transmission frame 86. A guide sleeve 873 is fixedly connected to one side of each support rod 872, and one side of the first guide rod 871 slides through the guide sleeve 873. When the first transmission frame 86 reciprocates under the action of the cam block 85, the first transmission frame 86 drives the first guide rods 871 on both sides to slide synchronously along the guide sleeves 873 on the support rods 872. The guide sleeves 873 restrict the sliding direction of the first guide rods 871. Through the cooperation of the double guide rods and the guide sleeves 873, the movement of the first transmission frame 86 is ensured to always remain horizontal, avoiding deviation or jamming, thus ensuring the stable oscillation of the chromatographic column 10. Simultaneously, the smooth structure inside the guide sleeves 873 reduces sliding friction and extends the service life of the assembly.

[0022] Please see Figure 5 and Figure 7 The second pusher mechanism 11 includes a second motor 111, which is fixedly connected inside the second mounting bracket 9. A rotating disk 112 is fixedly connected to the output end of the second motor 111. A second transmission rod 113 is fixedly connected to one side of the rotating disk 112. A second transmission frame 114 is provided on the outer side of the second transmission rod 113. A second guide assembly 115 is provided on the side of the second mounting bracket 9 near the second transmission frame 114. The second transmission frame 114 is slidably connected to one side of the second mounting bracket 9 via the second guide assembly 115. A connecting frame 1154 is fixedly connected to one side of the second guide assembly 115. A fixing mechanism 12 is located on one side of the connecting frame 1154. A second force-receiving groove 1141 is provided inside the second transmission frame 114. The second transmission rod 113 is slidably connected inside the second force groove 1141. By setting the second push column mechanism 11, after the second motor 111 is started, it drives the rotating disk 112 to rotate. The second transmission rod 113 on the rotating disk 112 moves in a circle with the rotating disk 112. At the same time, it slides in the second force groove 1141 of the second transmission frame 114. Through the force of the groove wall, the second transmission frame 114 is pushed to move reciprocally along the second guide assembly 115. Then, through the connecting frame 1154 and the fixing mechanism 12, the chromatographic column 10 is driven to swing longitudinally. The advantage of this design is that it forms an orthogonal swing direction with the first push column mechanism 8, so that the chromatographic column 10 generates multi-dimensional slight vibrations, which assist the sample flow from different directions and further reduce the risk of residue. At the same time, the eccentric transmission structure is simple and reliable and has low maintenance cost.

[0023] Please see Figure 7The second guide assembly 115 includes a limiting block 1151, which is fixedly connected to both sides of one end of the second mounting bracket 9. A second guide rod 1152 is fixedly connected between the two limiting blocks 1151. A slide block 1153 is slidably connected to the outer surface of the second guide rod 1152. One end of the slide block 1153 is fixedly connected to one end of the second transmission frame 114, and the other end of the slide block 1153 is fixedly connected to one end of the connecting bracket 1154. When the second transmission frame 114 moves under the action of the second transmission rod 113, it drives the slide block 1153 to slide back and forth along the second guide rod 1152. The limiting block 1151 restricts the sliding range of the slide block 1153 to avoid exceeding the stroke and causing structural damage. Through the sliding cooperation between the slide block 1153 and the second guide rod 1152, the movement accuracy of the second transmission frame 114 is ensured. The rigid connection between the slide block 1153 and the connecting bracket 1154 can transmit power to the chromatographic column 10 and ensure the oscillation effect.

[0024] Please see Figure 6 The fixing mechanism 12 includes a first slot 121, which is located on one side of the connecting frame 1154. A first insert block 122 is inserted into the first slot 121, and one side of the first insert block 122 is fixedly connected to one side of the assembly block 101. Second slots 123 are provided on both sides of the assembly block 101, and second insert blocks 124 are inserted into the second slots 123. A drive assembly 125 is provided on one side of the connecting frame 1154, and the second insert blocks 124 are fixedly connected to the second slots 123 via the drive assembly 125. The drive assembly 125 includes a fixing plate 1251, which is fixedly connected to the side of the connecting frame 1154 away from the assembly block 101. A third motor 1252 is fixedly connected to one side of the fixing plate 1251, and a bidirectional lead screw 1253 is fixedly connected to the output end of the third motor 1252. The bidirectional lead screw 1253 is rotatably connected to the fixed plate 1251. Inside the fixed plate 1251, both sides of the bidirectional lead screw 1253 are threadedly connected to moving blocks 1254. One side of the moving block 1254 is slidably connected inside the fixed plate 1251, and the second insert block 124 is fixedly connected to the moving block 1254. The first insert block 122 on the assembly block 101 is inserted into the first slot 121 of the connecting frame 1154 to achieve initial positioning. Then, the third motor 1252 is started to drive the bidirectional lead screw 1253 to rotate. The moving blocks 1254 on both sides of the bidirectional lead screw 1253 move closer together under the action of the threads, pushing the second insert block 124 into the second slot 123 of the assembly block 101 to complete the fixation. When disassembling, the third motor 1252 reverses to drive the bidirectional lead screw 1253 to rotate in the opposite direction. The moving block 1254 drives the second insert block 124 to disengage from the second slot 123, so that the chromatographic column 10 can be taken out. The chromatographic column 10 is firmly fixed through the double insertion structure, and the automated operation time is short.

[0025] Please see Figure 8 The connecting assembly 13 includes a first docking block 131, which is fixedly connected to the top end of the rubber liquid guiding sleeve 6. A second docking block 132 is fixedly connected to the bottom end of the chromatographic column 10. A stud 133 is fixedly connected to the top end of the first docking block 131. A sealing ring is fitted on the stud 133. The stud 133 is threadedly connected to the inside of the second docking block 132. When connecting the chromatographic column 10 and the rubber liquid guiding sleeve 6, the second docking block 132 is threadedly connected to the stud 133 on the first docking block 131 to complete the sealing docking. When disassembling, the two parts can be separated by rotating them in the opposite direction.

[0026] The working principle of this invention is as follows: First, the chromatographic column 10 is installed and fixed. The first insert 122 on the assembly block 101 on one side of the chromatographic column 10 is aligned with the first slot 121 of the connecting frame 1154 and inserted to achieve initial positioning. Then, the third motor 1252 is started. The third motor 1252 drives the bidirectional lead screw 1253 to rotate, so that the moving blocks 1254 on both sides of the lead screw move closer together, pushing the second insert 124 to be precisely inserted into the second slot 123 of the assembly block 101, thus completing the fixing of the chromatographic column 10 and the second pusher mechanism 11. Next, the chromatographic column 10 and the detection box 3 are sealed and connected. The screw hole of the second docking block 132 at the bottom of the chromatographic column 10 is aligned with the screw stud 133 of the first docking block 131 at the top of the rubber liquid guide sleeve 6. The chromatographic column 10 is rotated to make the threads of the two engage. Then, the detection process is started. The pretreated plant sample solution is injected into the chromatographic column 10, and the first pusher mechanism 8 and the second pusher mechanism 11 are activated at the same time. In the first pusher mechanism 8, the first motor 81 drives the drive gear 82. The rotation drives the driven gear 83 to reciprocate through the first transmission rod 84, causing the cam block 85 to push against the first transmission frame 86. This causes the first transmission frame 86 to move horizontally along the guide rod and guide sleeve 873 of the first guide assembly 87, thereby causing the chromatographic column 10 to swing laterally. The double-guide structure of the first guide assembly 87 ensures smooth swing without deviation. In the second column pushing mechanism 11, the second motor 111 drives the rotating disk 112 to rotate, causing the second transmission rod 113 to slide in the transmission groove of the second transmission frame 114. This pushes the second transmission frame 114 to reciprocate along the guide rod and slide block 1153 of the second guide assembly 115, causing the chromatographic column 10 to swing longitudinally. The limiting block 1151 strictly limits the stroke of the slide block 1153 to avoid structural damage. Under the action of the double column pushing mechanism, the chromatographic column 10 achieves multi-dimensional vibration, shortens the solution retention time, and greatly improves the transfer efficiency of the sample to the detection module. The organic selenium solution flows into the detection box 3 through the rubber liquid guide sleeve 6 and is then detected by the detection module.

[0027] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant sample organic selenium detection device, characterized in that, Including the detection platform (1), the top side of the detection platform (1) is fixedly connected with a detection box (3), one side of the detection box (3) is provided with a detection module, the detection module comprises a fluorescence sensing subunit (4) and a mass spectrum verification subunit (5), the fluorescence sensing subunit (4) and the mass spectrum verification subunit (5) are both installed on the detection box (3), the top of the detection box (3) is fixedly connected with a rubber liquid guide sleeve (6), the upper side of the rubber liquid guide sleeve (6) is provided with a chromatographic column (10), one side of the chromatographic column (10) is fixedly connected with an assembly block (101), one side of the detection platform (1) located at the detection box (3) is fixedly connected with a fixed frame (2), one side of the fixed frame (2) is fixedly connected with a first mounting frame (7), one side of the first mounting frame (7) is provided with a first push column mechanism (8), one side of the first mounting frame (7) is movably connected with a second mounting frame (9) through the first push column mechanism (8), one side of the second mounting frame (9) is provided with a second push column mechanism (11), one side of the second push column mechanism (11) is connected with one side of the chromatographic column (10), and a fixing mechanism (12) is arranged between the second push column mechanism (11) and the chromatographic column (10). A connecting assembly (13) is arranged between the rubber liquid guide sleeve (6) and the chromatographic column (10).

2. The device for detecting organic selenium in a plant sample according to claim 1, wherein, The first push column mechanism (8) comprises a first motor (81), the first motor (81) is fixedly connected to the first mounting frame (7), the output end of the first motor (81) is fixedly connected with a driving gear (82), one side of the driving gear (82) is meshedly connected with a driven gear (83), one side of the driven gear (83) is fixedly connected with a first transmission rod (84), one side of the first transmission rod (84) is fixedly connected with a cam block (85), the outer side of the cam block (85) is provided with a first transmission frame (86), one side of the fixed frame (2) is provided with a first guide assembly (87), the first transmission frame (86) is slidably connected to the fixed frame (2) through the first guide assembly (87), and one side of the first transmission frame (86) is fixedly connected with one side of the second mounting frame (9).

3. The device for detecting organic selenium in a plant sample according to claim 2, wherein, The first transmission frame (86) is internally provided with a first stress groove (861), and one side of the cam block (85) is in movable contact with the left and right inner walls of the first stress groove (861).

4. The device for detecting organic selenium in a plant sample according to claim 2, wherein, The first guide assembly (87) comprises a support rod (872), the support rod (872) is fixedly connected to the top of the fixed frame (2) on both sides, one side of the two support rods (872) is slidably connected with a first guide rod (871), the first guide rod (871) is fixedly connected to the left and right outer walls of the first transmission frame (86), one side of the support rod (872) is fixedly connected with a guide sleeve (873), and one side of the first guide rod (871) slidably penetrates through the guide sleeve (873).

5. The device for detecting organic selenium in a plant sample according to claim 1, wherein, The second push column mechanism (11) comprises a second motor (111), the second motor (111) is fixedly connected inside the second mounting frame (9), the output end of the second motor (111) is fixedly connected with a rotating disc (112), one side of the rotating disc (112) is fixedly connected with a second transmission rod (113), the outer side of the second transmission rod (113) is provided with a second transmission frame (114), one side of the second mounting frame (9) near the second transmission frame (114) is provided with a second guide assembly (115), the second transmission frame (114) is slidably connected on one side of the second mounting frame (9) through the second guide assembly (115), one side of the second guide assembly (115) is fixedly connected with a connecting frame (1154), and the fixing mechanism (12) is arranged on one side of the connecting frame (1154).

6. The device for detecting organic selenium in a plant sample according to claim 5, wherein, The second transmission frame (114) is internally provided with a second stress groove (1141), and the second transmission rod (113) is slidably connected in the second stress groove (1141).

7. The device for detecting organic selenium in a plant sample according to claim 5, wherein, The second guide assembly (115) comprises a limiting block (1151), the limiting block (1151) is fixedly connected to both sides of one end of the second mounting frame (9), a second guide rod (1152) is fixedly connected between the two limiting blocks (1151), a sliding seat (1153) is slidably connected to the outer surface of the second guide rod (1152), one end of the sliding seat (1153) is fixedly connected with one end of the second transmission frame (114), and the other end of the sliding seat (1153) is fixedly connected with one end of the connecting frame (1154).

8. The device for detecting organic selenium in a plant sample according to claim 5, wherein, The fixing mechanism (12) comprises a first insertion slot (121), the first insertion slot (121) is arranged on one side of the connecting frame (1154), a first insertion block (122) is inserted in the first insertion slot (121), one side of the first insertion block (122) is fixedly connected with one side of the assembly block (101), second insertion slots (123) are formed in the both sides of the assembly block (101), a second insertion block (124) is inserted in the second insertion slot (123), and a driving assembly (125) is arranged on one side of the connecting frame (1154).

9. The device for detecting organic selenium in a plant sample according to claim 8, wherein, The driving assembly (125) comprises a fixed plate (1251), the fixed plate (1251) is fixedly connected to the side of the connecting frame (1154) away from the assembly block (101), a third motor (1252) is fixedly connected to one side of the fixed plate (1251), a bidirectional screw rod (1253) is fixedly connected to the output end of the third motor (1252), the bidirectional screw rod (1253) is rotatably connected in the fixed plate (1251), moving blocks (1254) are threadedly connected to the both sides of the bidirectional screw rod (1253), one side of the moving block (1254) is slidably connected in the fixed plate (1251), and the second insertion block (124) is fixedly connected to the moving block (1254).

10. The device for detecting organic selenium in a plant sample according to claim 1, wherein, The connecting assembly (13) comprises a first docking block (131) fixedly connected to the top end of the rubber liquid guide sleeve (6), the bottom end of the chromatographic column (10) is fixedly connected with a second docking block (132), the top end of the first docking block (131) is fixedly connected with a threaded stud (133), and the threaded stud (133) is threadedly connected in the second docking block (132).

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