Traditional Chinese medicine composition for removing acnes

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions, and provides a traditional Chinese medicine composition for removing acnes, which is prepared from the following components in parts by weight: 1-2 parts of honeysuckle stem extract, 0.3-0.8 part of fructus forsythiae extract, 2-4 parts of radix scutellariae extract, 0.5-3.5 parts of cortex magnoliae officinalis extract and 1-5 parts of astragalus complanatus extract. The preparation method of the fructus forsythiae extract comprises the following steps: crushing fructus forsythiae, performing steam explosion treatment to obtain pretreated fructus forsythiae, adding water, adding biological compound enzyme, performing enzymolysis, filtering and performing enzyme deactivation to obtain filtrate; enabling the filtrate to pass through macroporous resin, washing with water, carrying out first elution to obtain a solution I, and carrying out second elution to obtain a solution II; performing post-treatment on the solution I to obtain forsythiaside; performing post-treatment on the solution II to obtain forsythin; the biological compound enzyme comprises alpha-galactosidase, lipase and xylanase. Through the technical scheme, the problem of low extraction rate of forsythin and forsythiaside in fructus forsythiae in the prior art is solved.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine composition technology, specifically to a traditional Chinese medicine composition for treating acne. Background Technology

[0002] Acne, commonly known as pimples, is a widespread chronic inflammatory skin disease of the sebaceous glands. It can even develop into serious skin problems, primarily affecting the face, chest, and back—areas with high sebum production. Acne lesions can lead to facial scarring in some patients, and the longer the acne persists, the more severe the scarring. Acne is classified into non-inflammatory and inflammatory types. Some non-inflammatory acne can gradually worsen into inflammatory acne. While inflammatory acne can resolve, it can leave pitted or hypertrophic scars on the face, severely affecting facial aesthetics.

[0003] Currently, acne treatment methods are mainly divided into Western medicine treatment and traditional Chinese medicine treatment. Western medicine treatment mainly uses some chemical drugs to intervene and play an antibacterial and anti-inflammatory role. Although the above drugs can play a role in removing acne, they may irritate the skin or make it more prone to allergies. Therefore, it is crucial to find a gentler combination of traditional Chinese medicine.

[0004] Forsythia, a common traditional Chinese medicine, contains forsythoside and forsythoside ester, both of which have antibacterial and anti-inflammatory effects and are often used in acne treatment. However, traditional extraction methods result in low yields and damage to these components. Therefore, researching more efficient extraction methods for forsythoside and forsythoside ester is crucial. Summary of the Invention

[0005] This invention proposes a traditional Chinese medicine composition for acne treatment, which solves the problem of low extraction rates of forsythoside and forsythoside ester in traditional Chinese medicine compositions for acne treatment in related technologies.

[0006] The technical solution of the present invention is as follows: This invention proposes a traditional Chinese medicine composition for acne treatment, comprising the following components in parts by weight: 1-2 parts of honeysuckle vine extract, 0.3-0.8 parts of forsythia extract, 2-4 parts of scutellaria extract, 0.5-3.5 parts of magnolia bark extract, and 1-5 parts of astragalus root extract. The preparation method of the Forsythia extract includes the following steps: S1. Crush the forsythia into powder and subject it to steam explosion treatment to obtain pretreated forsythia. S2. Mix the pretreated Forsythia suspensa with water, add biological complex enzyme, hydrolyze, filter, inactivate enzyme, and obtain filtrate; S3. Pass the filtrate through a macroporous resin, wash with water, and then perform a first elution to obtain solution I. Perform a second elution to obtain solution II. S4. Concentrate, extract, and dry the solution I to obtain forsythoside; concentrate, separate the precipitate, and dry the solution II to obtain forsythoside. The biocomplex enzyme includes α-galactosidase, lipase, and xylanase.

[0007] As a further technical solution, the pressure of the steam explosion treatment is 1.0~1.5MPa, and the time is 5~10min.

[0008] As a further technical solution, the mass ratio of Forsythia suspensa to water after the pre-treatment for steam explosion is 1:5~10.

[0009] As a further technical solution, the mass ratio of α-galactosidase, lipase and xylanase is 2:0.5:0.5~1.5.

[0010] As a further technical solution, the mass ratio of the pretreated Forsythia suspensa to the biocomplex enzyme is 200:1~2.

[0011] As a further technical solution, the enzymatic hydrolysis temperature is 40~50℃ and the time is 2~4h.

[0012] As a further technical solution, the enzyme inactivation temperature is 80~85℃ and the time is 15~25min.

[0013] As a further technical solution, the macroporous resin includes HPD100 macroporous resin and X-5 macroporous resin.

[0014] In this invention, HPD100 macroporous resin and X-5 macroporous resin are used together to further improve the extraction rate of forsythosides and forsythoside esters from Forsythia suspensa.

[0015] As a further technical solution, the flow rate of the filtrate through the macroporous resin is 1500~2000 L / m. 2 ·h.

[0016] As a further technical solution, the first elution uses an ethanol solution with a volume concentration of 25% to 35%.

[0017] As a further technical solution, the second elution uses an ethanol solution with a volume concentration of 50% to 60%.

[0018] As a further technical solution, the concentration is carried out under reduced pressure, and the temperature of the reduced pressure concentration is 45~55℃.

[0019] As a further technical solution, the extractant used in the extraction includes one or both of n-butanol solution and petroleum ether.

[0020] As a further technical solution, the drying temperature is 45~55℃.

[0021] The working principle and beneficial effects of this invention are as follows: In this invention, the honeysuckle vine extract contains active ingredients such as chlorogenic acid, among which chlorogenic acid has antibacterial and anti-inflammatory effects, which can effectively control bacterial infection in acne areas, relieve inflammation, and reduce the occurrence and aggravation of acne. Forsythia extract contains forsythoside and forsythoside ester. Forsythoside has antibacterial, anti-inflammatory and immunomodulatory effects; forsythoside ester has antibacterial, anti-inflammatory and antioxidant effects; both can prevent and treat acne and help repair damaged skin tissue. Scutellaria baicalensis extract contains baicalin, baicalein, etc. Baicalin has significant anti-inflammatory effects, as well as antibacterial and antioxidant effects, which can improve the condition of acne-prone skin and prevent acne marks and scars. Magnolia bark extract contains magnolol, which not only has antibacterial and anti-inflammatory properties but also regulates the skin's oil balance, making it particularly effective for people with oily skin and acne, keeping the skin refreshed. Astragalus membranaceus extract contains a variety of flavonoids and other components. These flavonoids have multiple functions such as anti-oxidation, anti-inflammation and immune regulation, which can improve the overall health of the skin and reduce acne recurrence. When extracting forsythosides and forsythosides esters from Forsythia suspensa, the use of α-galactosidase, lipase, and xylanase as a bio-complex enzyme significantly improves the extraction rate of forsythosides and forsythosides esters. α-galactosidase can act on the polysaccharides in the cell wall of Forsythia suspensa, disrupting the cell wall. Xylanase degrades the hemicellulose components in the cell wall, directly disrupting the cell wall's integrity. The addition of lipase can disrupt the cell membrane structure. When all three are used together, the extraction process is optimized, greatly improving the dissolution rate of forsythosides and forsythosides esters. This increased extraction rate allows for the addition of active ingredients in traditional Chinese medicine compositions for acne treatment, enhancing the acne-reducing effect and reducing the risk of impurities causing other skin problems. Attached Figure Description

[0022] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0023] Figure 1 The photos show before and after some patients used the acne-removing traditional Chinese medicine preparation of the present invention. (a) and (c) are photos before use, and (b) and (d) are photos after use. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0025] In the following embodiments and comparative examples, Honeysuckle vine extract: manufactured by Shaanxi Xintianyu Biotechnology Co., Ltd.; Scutellaria baicalensis extract: manufactured by Shaanxi Xintianyu Biotechnology Co., Ltd.; Magnolia officinalis bark extract: manufactured by Jiangsu Jufeng Chemical Technology Co., Ltd.; Astragalus membranaceus extract: brand name MedChemExpress (MCE); α-Galactosidase: Model number FDG-2239, manufacturer: Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd. Lipase: Model number FDG-2208, manufacturer: Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd. Xylanase: The manufacturer is Jiangsu Caiwei Biotechnology Co., Ltd.

[0026] Example 1 A traditional Chinese medicine composition for treating acne includes the following components in parts by weight: 1 part honeysuckle vine extract, 0.8 parts forsythia extract, 2 parts scutellaria extract, 3.5 parts magnolia bark extract, and 5 parts astragalus root extract. The preparation method of Forsythia suspensa extract includes the following steps: After washing away mud and impurities, 1 kg of Forsythia suspensa was crushed. The crushed Forsythia suspensa was then subjected to steam explosion treatment at a pressure of 1.0 MPa for 10 minutes. Forsythia suspensa treated with steam explosion was mixed with water, and then a bio-complex enzyme was added. The mixture was enzymatically hydrolyzed at 40°C for 4 hours, filtered, and the enzyme was inactivated at 80°C for 25 minutes to obtain the filtrate. The mass ratio of steam-exploded forsythia suspensa to water was 1:5, the mass ratio of steam-exploded forsythia suspensa to bio-complex enzyme was 200:1.5, and the mass ratio of α-galactosidase, lipase and xylanase in the bio-complex enzyme was 2:0.5:0.2. The filtrate was discharged at 1500 L / m 2 The sample was passed through HPD macroporous resin at a speed of h, and the impurities remaining in the HPD macroporous resin were washed away with deionized water. Then, it was eluted with a 25% (v / v) ethanol solution to obtain forsythoside extract; then washed with a 50% (v / v) ethanol solution to obtain forsythoside extract. The forsythoside extract was concentrated under reduced pressure, extracted twice with a water-saturated n-butanol solution, and the residue was dried at 45°C to obtain forsythoside. The forsythoside extract was concentrated under reduced pressure at 45°C, the concentrate was allowed to stand, the precipitate was separated, and dried at 55°C to obtain forsythoside. A method for preparing a traditional Chinese medicine composition for acne treatment includes the following steps: The extracts of honeysuckle vine, forsythia, scutellaria, magnolia bark, and astragalus root were mixed evenly to obtain a traditional Chinese medicine composition for acne treatment.

[0027] Example 2 A traditional Chinese medicine composition for treating acne includes the following components in parts by weight: 2 parts of honeysuckle vine extract, 0.3 parts of forsythia extract, 4 parts of scutellaria extract, 0.5 parts of magnolia bark extract, and 1 part of astragalus root extract. The preparation method of Forsythia suspensa extract includes the following steps: After washing away mud and impurities, 1 kg of Forsythia suspensa was crushed. The crushed Forsythia suspensa was then subjected to steam explosion treatment at a pressure of 1.5 MPa for 5 minutes. Forsythia suspensa treated with steam explosion was moistened with water, and then a bio-complex enzyme was added. The mixture was enzymatically hydrolyzed at 50°C for 2 hours, filtered, and the enzyme was inactivated at 85°C for 20 minutes to obtain the filtrate. The mass ratio of steam-exploded forsythia suspensa to water was 1:10, the mass ratio of steam-exploded forsythia suspensa to bio-complex enzyme was 200:1.5, and the mass ratio of α-galactosidase, lipase and xylanase in the bio-complex enzyme was 2:0.5:2. The filtrate was discharged at 2000 L / m 2 The sample was passed through HPD macroporous resin at a speed of h, and the impurities remaining in the HPD macroporous resin were washed away with deionized water. Then, it was eluted with a 35% (v / v) ethanol solution to obtain forsythoside extract; then washed with a 60% (v / v) ethanol solution to obtain forsythoside extract. The forsythoside extract was concentrated under reduced pressure, extracted twice with a water-saturated n-butanol solution, and the residue was dried at 55°C to obtain forsythoside. The forsythoside extract was concentrated under reduced pressure at 55°C, the concentrate was allowed to stand, the precipitate was separated, and dried at 55°C to obtain forsythoside. A method for preparing a traditional Chinese medicine composition for acne treatment includes the following steps: The extracts of honeysuckle vine, forsythia, scutellaria, magnolia bark, and astragalus root were mixed evenly to obtain a traditional Chinese medicine composition for acne treatment.

[0028] Example 3 A traditional Chinese medicine composition for treating acne includes the following components in parts by weight: 1.6 parts of honeysuckle vine extract, 0.5 parts of forsythia extract, 3 parts of scutellaria extract, 2 parts of magnolia bark extract, and 2 parts of astragalus root extract. The preparation method of Forsythia suspensa extract includes the following steps: After washing away mud and impurities, 1 kg of Forsythia suspensa was crushed. The crushed Forsythia suspensa was then subjected to steam explosion treatment at a pressure of 1.2 MPa for 8 minutes. Forsythia suspensa treated with steam explosion was moistened with water, and then a bio-complex enzyme was added. The mixture was enzymatically hydrolyzed at 45°C for 2.5 hours, filtered, and the enzyme was inactivated at 83°C for 20 minutes to obtain the filtrate. The mass ratio of steam-exploded forsythia suspensa to water was 1:8, the mass ratio of steam-exploded forsythia suspensa to bio-complex enzyme was 200:1.5, and the mass ratio of α-galactosidase, lipase and xylanase in the bio-complex enzyme was 2:0.5:1.8. The filtrate was discharged at 1800 L / m 2 The sample was passed through HPD macroporous resin at a speed of h, and the impurities remaining in the HPD macroporous resin were washed away with deionized water. Then, it was eluted with a 30% (v / v) ethanol solution to obtain forsythoside extract; then, it was washed with a 55% (v / v) ethanol solution to obtain forsythoside extract. The forsythoside extract was concentrated under reduced pressure, extracted twice with a water-saturated n-butanol solution, and the residue was dried at 50°C to obtain forsythoside. The forsythoside extract was concentrated under reduced pressure at 50°C, the concentrate was allowed to stand, the precipitate was separated, and dried at 50°C to obtain forsythoside. A method for preparing a traditional Chinese medicine composition for acne treatment includes the following steps: The extracts of honeysuckle vine, forsythia, scutellaria, magnolia bark, and astragalus root were mixed evenly to obtain a traditional Chinese medicine composition for acne treatment.

[0029] Example 4 The only difference between this embodiment and Example 3 is that the mass ratio of α-galactosidase, lipase and xylanase in the biological complex enzyme used is 2:0.5:1.5.

[0030] Example 5 The only difference between this embodiment and Example 3 is that the mass ratio of α-galactosidase, lipase and xylanase in the biological complex enzyme used is 2:0.5:0.5.

[0031] Example 6 The only difference between this embodiment and Example 3 is that the mass ratio of α-galactosidase, lipase and xylanase in the biological complex enzyme used is 2:0.5:0.3.

[0032] Example 7 The difference between this embodiment and Embodiment 5 is that the HPD macroporous resin is replaced with X-5 macroporous resin.

[0033] Example 8 The only difference between this embodiment and Example 5 is the preparation method of the Forsythia extract, which includes the following steps: After washing away mud and other impurities, 1 kg of Forsythia suspensa was crushed. The crushed Forsythia suspensa was then subjected to steam explosion treatment at a pressure of 1.2 MPa for 8 minutes. The steam-exploded forsythia was moistened with water, with a mass ratio of steam-exploded forsythia to water of 7:3. Then, a bio-complex enzyme was added, with a mass ratio of steam-exploded forsythia to bio-complex enzyme of 200:1.5. The mass ratio of α-galactosidase, lipase and xylanase in the bio-complex enzyme was 2:0.5:1.8. The enzyme was enzymatically hydrolyzed at 50℃ for 2.5h, filtered, and the enzyme was inactivated at 80℃ for 20min to obtain the filtrate. The filtrate was discharged at 1500 L / m 2 The solution was passed through HPD macroporous resin at a rate of 1500 L / m³. 2 The mixture is then passed through X-5 macroporous resin at a speed of h. Impurities remaining in both HPD macroporous resin and X-5 macroporous resin are washed away with deionized water. The mixture is then eluted with 30% ethanol solution I to obtain forsythoside extract. Extraction and drying yield forsythoside; the extract is then washed with 55% ethanol solution II to obtain solution II. Solution I was concentrated under reduced pressure, allowed to stand, and the supernatant was collected. Solution II was concentrated under reduced pressure, and the precipitate was separated to obtain forsythoside.

[0034] Comparative Example 1 The only difference between this comparative example and Example 3 is that the mass ratio of α-galactosidase to lipase in the bio-complex enzyme used is 2:0.5.

[0035] Comparative Example 2 The only difference between this comparative example and Example 3 is that the mass ratio of α-galactosidase to xylanase in the bio-complex enzyme used is 2:1.8.

[0036] Comparative Example 3 The only difference between this comparative example and Example 3 is that the mass ratio of lipase to xylanase in the biocomplex enzyme used is 0.5:1.8.

[0037] The contents and extraction rates of forsythoside and forsythoside esters extracted in Examples 1-8 and Comparative Examples 1-3 were calculated: The calculation results are shown in Table 1: Table 1. Calculation results of the content and extraction rate of forsythoside and forsythoside extracted in Examples 1-8 and Comparative Examples 1-3.

[0038] 1. Compared with Comparative Examples 1-3, Examples 1-8 showed higher contents of forsythoside and forsythoside esters extracted from Forsythia suspensa, indicating that the combined use of α-galactosidase, lipase and xylanase improved the extraction rate of forsythoside and forsythoside esters from Forsythia suspensa.

[0039] 2. Compared with Examples 3-6, the contents of forsythoside and forsythoside extracted in Examples 4-5 were higher than those in Examples 3 and 6, indicating that further regulating the mass ratio of α-galactosidase, lipase and xylanase to 2:0.5:0.5-1.5 significantly improved the extraction rate of forsythoside and forsythoside from Forsythia.

[0040] 3. Compared with Examples 5 and 7-8, the content of forsythoside and forsythoside extracted in Example 8 was significantly higher than that in Examples 5 and 7, indicating that using HPD100 macroporous resin and X-5 macroporous resin together during the extraction process helps to improve the extraction rate of forsythoside and forsythoside from Forsythia.

[0041] Experimental Example 1 1.1 Traditional Chinese Medicine Preparations for Acne Treatment The acne-removing traditional Chinese medicine preparation is an ointment, lotion, or gel, and contains the acne-removing traditional Chinese medicine composition obtained in Example 8 above.

[0042] 1.2 Acne Treatment Efficacy Experiment One hundred young people aged 18-25 with facial papules, pustules, nodules, cysts and scars (gender not limited) were selected and divided into two groups of 50 each, designated as Group 1 and Group 2, to serve as the experimental subjects for the product. Instructions for use: Subjects should apply the acne-removing Chinese herbal preparation to the affected area for half an hour after cleansing their face in the morning and evening. The efficacy will be evaluated after 28 days. Efficacy evaluation criteria: Based on the "Guiding Principles for Clinical Research of New Traditional Chinese Medicines for the Treatment of Acne Vulgaris: Efficacy Judgment Criteria" published by China Medical Science and Technology Press in 2002; Cure: Skin lesions recede at a rate of ≥95%, leaving only pigmentation and no new lesions appearing; Effective: Skin lesion regression rate ≥50%, but new skin lesions still appear; Ineffective: Skin lesion regression rate <50%, insufficient reduction of skin lesions, or even an increase.

[0043] The test results are shown in Table 2: Table 2. Acne Treatment Efficacy Experiment

[0044] Based on the above results, the acne-removing traditional Chinese medicine preparation made from the acne-removing traditional Chinese medicine composition of the present invention has a total effective rate of 99% in treating acne, indicating that the traditional Chinese medicine composition of the present invention has excellent acne-removing efficacy.

[0045] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for treating acne, characterized in that, It includes the following components in parts by weight: 1-2 parts of honeysuckle vine extract, 0.3-0.8 parts of forsythia extract, 2-4 parts of scutellaria extract, 0.5-3.5 parts of magnolia bark extract, and 1-5 parts of astragalus root extract; The preparation method of the Forsythia extract includes the following steps: S1. Crush the forsythia into powder and subject it to steam explosion treatment to obtain pretreated forsythia. S2. Mix the pretreated Forsythia suspensa with water, add biological complex enzyme, hydrolyze, filter, inactivate enzyme, and obtain filtrate; S3. Pass the filtrate through a macroporous resin, wash with water, and then perform a first elution to obtain solution I. Perform a second elution to obtain solution II. S4. Concentrate, extract, and dry the solution I to obtain forsythoside; concentrate, separate the precipitate, and dry the solution II to obtain forsythoside. The biocomplex enzyme includes α-galactosidase, lipase, and xylanase.

2. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The pressure for the steam explosion treatment is 1.0~1.5MPa, and the time is 5~10min.

3. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The mass ratio of forsythia to water after the pre-treatment for steam explosion is 1:5~10.

4. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The mass ratio of α-galactosidase, lipase and xylanase is 2:0.5:0.5~1.

5.

5. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The mass ratio of the pretreated Forsythia to the biocomplex enzyme is 200:1~2.

6. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The enzymatic hydrolysis is performed at a temperature of 40-50℃ for 2-4 hours.

7. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The enzyme inactivation temperature is 80~85℃, and the time is 15~25min.

8. The traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The macroporous resins include HPD100 macroporous resin and X-5 macroporous resin.

9. A traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The flow rate of the filtrate through the macroporous resin is 1500~2000 L / m. 2 ·h; The first elution uses an ethanol solution with a volume concentration of 25% to 35%; The second elution uses an ethanol solution with a volume concentration of 50% to 60%.

10. A traditional Chinese medicine composition for acne treatment according to claim 1, characterized in that, The extractant used in the extraction process includes one or both of n-butanol solution and petroleum ether.

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