Ecological restoration method for moderately degenerated Coilin grassland
By reseeding sand-loving grass and sand fern seeds and applying nitrogen and phosphorus mixed fertilizer in the Horqin Grassland, the vegetation restoration problem of moderately degraded grassland has been solved, and the grassland ecology and soil quality have been improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2026-03-24
AI Technical Summary
The Horqin Grassland has suffered moderate degradation due to factors such as climate change, reclamation, overgrazing, and harvesting. This is manifested in reduced grass yield, decreased utilization rate, frequent biological disasters, and an increase in natural disasters. Vegetation restoration and ecological restoration have become important tasks.
On the moderately degraded Horqin grassland, a mixture of sand-loving ice grass and sand fern was sown at a mass ratio of 2.5-3.5:1, along with nitrogen and phosphorus mixed fertilizer with a nitrogen:phosphorus ratio of 1:1, to optimize soil chemical properties.
It restored annual and biennial plants in moderately degraded grasslands, enhanced the growth of perennial plants, brought soil pH to a neutral level, increased total nitrogen content in the soil, and restored vegetation.
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Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of degenerated grassland ecological management and restoration, and particularly relates to a method for restoring the ecology of moderately degenerated Horqin grassland. BACKGROUND
[0002] The Horqin grassland spans Inner Mongolia, Jilin and Liaoning provinces, and is located at the intersection of the Northeast Plain and Inner Mongolia Plateau, the semi-humid region and the semi-arid region, and the agricultural and animal husbandry transition zone, with a total area of 768×105hm 2 in Inner Mongolia, distributed in Hinggan League, Tongliao City and Chifeng City. Tongliao City is located in the belly of the Horqin grassland, with a total grassland area of 314×104hm 2 , of which the available grassland area is 312×104hm 2 . The original vegetation of the Horqin grassland belongs to the forest-grassland transition type. Due to natural factors such as climate change and human factors such as large-scale reclamation, overgrazing, excessive collection of Mongolian medicinal herbs, and mining, the Horqin grassland has been degenerated and desertified to varying degrees. The species composition of the community has changed greatly, the dominant species of the grassland has decreased, and the number of xerophytes and annual plants has increased greatly, even becoming the main plants. The ecological health of the grassland is under great test. In fact, the Horqin grassland has evolved into a “Horqin sandy land”, which is characterized by reduced grass yield, decreased utilization rate, decreased carrying capacity, frequent biological disasters, and increased natural disasters. Therefore, as an important part of the ecological security barrier in the north, the restoration of vegetation and the ecological restoration of the Horqin grassland have become an urgent task. SUMMARY
[0003] A method for restoring the ecology of moderately degenerated Horqin grassland, comprising the following steps: S1, investigation: investigate the regional conditions, climate characteristics, vegetation conditions and soil conditions of the Horqin grassland, and determine the moderately degenerated region of the Horqin grassland; S2, investigation of plant community characteristics and biomass: in the lush period of pasture growth from July to August, the moderately degenerated Horqin grassland sample site is investigated, the height, coverage and density of the plant community of the Horqin grassland are monitored, and data are collected; S3, determination of plant community pasture quality: the water content, plant protein, plant ash, plant fat, neutral detergent fiber and acid detergent fiber of the plant community pasture of the moderately degenerated Horqin grassland are determined; S4, collection of soil samples: soil sampling is performed in August to determine the soil chemical property indexes, soil bacterial community species composition and soil fungal community species composition; S5, complementary sowing: complementary sowing of grass seeds on the moderately degraded Horqin grassland, the complementary sowing grass seeds being Agropyron mongolicum and Hedysarum leave, the mass ratio of Agropyron mongolicum to Hedysarum leave being 2.5-3.5:1, and the complementary sowing amount being 30-50 kg / hm 2 , the sowing depth being 1-2 cm, and the continuous complementary sowing being performed for two years S6, fertilization: fertilization is performed in the second year after the complementary sowing, the fertilizer package being 150-210 kg / hm 2 before the precipitation, the fertilizer package being applied by artificial scattering, the fertilizer package being a nitrogen-phosphorus mixed fertilizer, the nitrogen-phosphorus mixed fertilizer comprising nitrogen fertilizer and phosphorus fertilizer, the mass ratio of nitrogen to phosphorus in the nitrogen-phosphorus mixed fertilizer being 1:1.
[0004] Preferably, the complementary sowing grass seeds are Agropyron mongolicum and Hedysarum leave, the mass ratio of Agropyron mongolicum to Hedysarum leave being 3:1.
[0005] Preferably, the complementary sowing grass seeds are Agropyron mongolicum and Hedysarum leave, the mass ratio of Agropyron mongolicum to Hedysarum leave being 2.5:1.
[0006] Preferably, the complementary sowing grass seeds are Agropyron mongolicum and Hedysarum leave, the mass ratio of Agropyron mongolicum to Hedysarum leave being 3.5:1.
[0007] Preferably, the nitrogen fertilizer is urea, and the phosphorus fertilizer is heavy superphosphate.
[0008] Preferably, the nitrogen content of the urea is greater than or equal to 46 %, and the phosphorus content of the phosphorus fertilizer is greater than or equal to 44 %.
[0009] Preferably, in the step S3, the water content is determined by an oven drying method, plant protein is determined by a full-automatic nitrogen determination instrument, plant ash is determined by a muffle furnace, plant fat is determined by a fat instrument, neutral detergent fiber is determined by a FOSS Fibertec 2010 full-automatic fiber analysis system, and acid detergent fiber is determined by a FOSS Fibertec 2010 full-automatic fiber analysis system.
[0010] Preferably, in the step S4, the soil chemical property indexes include soil total nitrogen, total phosphorus, available potassium and organic matter content.
[0011] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the soil bacterial community species composition in the Horqin degraded typical grassland soil sample.
[0012] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the soil fungal community species composition in the Horqin degraded typical grassland soil sample.
[0013] In the present application, the Horqin grassland is moderately degraded when the degradation degree reaches the following: The quantity of excellent forage grasses in the grass layer is obviously reduced, and inferior forage grasses or poisonous and harmful plants are relatively increased; The grassland is successively degraded to low yield and low quality, and the soil structure and physicochemical properties are obviously deteriorated; The forage grass yield is further reduced by 30%-40%, and the forage supply for grazing livestock begins to be insufficient; The ground surface may be slightly eroded, covered with sand or gravel; The ecological function of the grassland begins to be damaged, but the overall ecological system can still maintain a certain stability.
[0014] Compared with the prior art, the method has the following beneficial effects: According to the plant community conditions of the moderately degraded Horqin grassland, suitable grass seeds and fertilizer bags are screened out for reseeding and fertilization, the reseeding grass seeds are Agropyron desertorum and Anemone raddeana, and the mass ratio of Agropyron desertorum to Anemone raddeana is 2.5-3.5:1; the nitrogen-phosphorus mixed fertilizer is used for fertilization, the mass ratio of nitrogen to phosphorus in the nitrogen-phosphorus mixed fertilizer is 1:1, the one-year and two-year plants in the Horqin grassland are reduced, the perennial grasses, forbs and perennial legumes are grown, the soil pH value tends to be neutral, the total nitrogen content of the soil is increased by fertilization, and the moderately degraded Horqin grassland vegetation is restored. DETAILED DESCRIPTION Embodiment 1
[0015] A method for ecological restoration of a moderately degraded Horqin grassland, comprising the following steps: S1, investigation: investigating the regional general situation, climate characteristics, vegetation general situation and soil general situation of the Horqin grassland, and determining the moderately degraded region of the Horqin grassland; S2, investigation of plant community characteristics and biomass: in July, the forage grass growth period, the moderately degraded Horqin grassland sample land is investigated, the height, coverage and density of the Horqin grassland plant community are monitored, and data are collected; S3, determination of forage grass quality of the plant community: the forage grass quality of the plant community of the moderately degraded Horqin grassland is determined, including water content, plant protein, plant ash, plant fat, neutral detergent fiber and acid detergent fiber; S4, collection of soil samples: soil sampling is performed in August, and the soil chemical property indexes, soil bacterial community species composition and soil fungal community species composition are determined; S5, reseeding: reseeding grass seeds on the moderately degraded Horqin grassland, the reseeding grass seeds are Agropyron desertorum and Anemone raddeana, and the mass ratio of Agropyron desertorum to Anemone raddeana is 2.5:1; the reseeding amount is 30 kg / hm 2 ; the sowing depth is 1 cm, and the reseeding is continuously performed for two years; S6, fertilization: fertilization is performed in the second year after reseeding, and the fertilizer bag 150 kg / hm 2The fertilizer package is a nitrogen-phosphorus mixed fertilizer, which comprises a nitrogen fertilizer and a phosphorus fertilizer, and the nitrogen-phosphorus mixed fertilizer has a ratio of nitrogen to phosphorus of 1:1.
[0016] Preferably, the nitrogen fertilizer is urea, and the phosphorus fertilizer is heavy superphosphate.
[0017] Preferably, the nitrogen content of the urea is greater than or equal to 46%, and the phosphorus fertilizer contains phosphorus pentoxide in an amount greater than or equal to 44%.
[0018] Preferably, in the step S3, the water content is determined by a drying method, the plant protein is determined by a full-automatic nitrogen determination instrument, the plant ash is determined by a muffle furnace, the plant fat is determined by a fat instrument, the neutral detergent fiber is determined by a FOSS Fibertec 2010 full-automatic fiber analysis system, and the acid detergent fiber is determined by a FOSS Fibertec 2010 full-automatic fiber analysis system.
[0019] Preferably, in the step S4, the soil chemical property index comprises the contents of total nitrogen, total phosphorus, available potassium and organic matter of the soil.
[0020] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the species composition of the soil bacterial community in the soil sample of the degraded typical grassland in Horqin.
[0021] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the species composition of the soil fungal community in the soil sample of the degraded typical grassland in Horqin. Embodiment 2
[0022] A method for ecological restoration of a moderately degraded grassland in Horqin, comprising the following steps: S1, investigation: investigating the regional overview, climate characteristics, vegetation overview and soil overview of the grassland in Horqin, and determining the moderately degraded area of the grassland in Horqin; S2, investigation of plant community characteristics and biomass: in August, when the pasture is growing vigorously, investigating the sample land of the moderately degraded grassland in Horqin, monitoring the height, coverage and density of the plant community of the grassland in Horqin, and collecting data; S3, determination of pasture quality of plant community: determining the water content, plant protein, plant ash, plant fat, neutral detergent fiber and acid detergent fiber of the pasture quality of the plant community of the moderately degraded grassland in Horqin; S4, collection of soil samples: collecting soil samples in August, and determining the soil chemical property index, the species composition of the soil bacterial community and the species composition of the soil fungal community; S5, supplemental sowing: on the moderately degraded Horqin grassland, supplemental sowing of grass seeds, the supplemental sowing grass seeds being psammophytic agropyron and psammophytic elytrum, in a mass ratio of psammophytic agropyron: psammophytic elytrum = 3.5:1, the supplemental sowing amount being 50 kg / hm 2 ; the sowing depth being 2 cm, and the supplemental sowing being continuously performed for two years; S6, fertilization: in the second year after the supplemental sowing, fertilization is performed, before the rainfall, by means of artificial spreading of a fertilizer package 210 kg / hm 2 , the fertilizer package being a nitrogen-phosphorus mixed fertilizer, the nitrogen-phosphorus mixed fertilizer including a nitrogen fertilizer and a phosphorus fertilizer, in the nitrogen-phosphorus mixed fertilizer, the mass ratio of nitrogen to phosphorus being 1:1.
[0023] Preferably, the nitrogen fertilizer is urea, and the phosphorus fertilizer is heavy superphosphate.
[0024] Preferably, the nitrogen content of the urea is ≥46 %, and the phosphorus content of the phosphorus fertilizer is ≥44 %.
[0025] Preferably, in the step S3, the water content is determined by means of an oven drying method, the plant protein is determined by means of a full-automatic nitrogen determination instrument Kjeldahl method, the plant ash is determined by means of a muffle furnace, the plant fat is determined by means of a fat instrument, the neutral detergent fiber is determined by means of a FOSS Fibertec 2010 full-automatic fiber analysis system, and the acid detergent fiber is determined by means of a FOSS Fibertec 2010 full-automatic fiber analysis system.
[0026] Preferably, in the step S4, the soil chemical property indexes include soil total nitrogen, total phosphorus, available potassium, and organic matter content.
[0027] Preferably, in the step S4, a high-throughput sequencing technology is used to analyze the soil bacterial community species composition in the Horqin degraded typical grassland soil sample.
[0028] Preferably, in the step S4, a high-throughput sequencing technology is used to analyze the soil fungal community species composition in the Horqin degraded typical grassland soil sample. Embodiment 3
[0029] A method for ecological restoration of a moderately degraded Horqin grassland, comprising the following steps: S1, investigation: investigating the regional general situation, climate characteristics, vegetation general situation, and soil general situation of the Horqin grassland, and determining a moderately degraded region of the Horqin grassland; S2, investigation of plant community characteristics and biomass: in the growth peak period of pasture in August, a sample plot of the moderately degraded Horqin grassland is investigated, the height, coverage, and density of the plant community of the Horqin grassland are monitored, and data are collected; S3, Forage quality determination of plant community in moderately degraded Horqin grassland: moisture content, plant protein, plant ash, plant fat, neutral detergent fiber, acid detergent fiber of forage quality of plant community in moderately degraded Horqin grassland are determined; S4, Collection of soil samples: soil sampling is carried out in August to determine soil chemical property indexes, soil bacterial community species composition, and soil fungal community species composition; S5, Supplementary sowing: grass seeds are supplemented in the moderately degraded Horqin grassland, the supplemented grass seeds are psammophytic agropyron and psammophytic elytrum, and the mass ratio of psammophytic agropyron to psammophytic elytrum is 3:1; the supplementary sowing amount is 40 kg / hm 2 ; the sowing depth is 1.5 cm, and the supplementary sowing is continuously carried out for two years; S6, Fertilization: fertilization is carried out in the second year after the supplementary sowing, and 180 kg / hm 2 of fertilizer packages are applied by artificial scattering before precipitation, the fertilizer packages are nitrogen-phosphorus mixed fertilizer, the nitrogen-phosphorus mixed fertilizer comprises nitrogen fertilizer and phosphorus fertilizer, and the mass ratio of nitrogen to phosphorus in the nitrogen-phosphorus mixed fertilizer is 1:1.
[0030] Preferably, the nitrogen fertilizer is urea, and the phosphorus fertilizer is heavy superphosphate.
[0031] Preferably, the nitrogen content of the urea is greater than or equal to 46 %, and the phosphorus fertilizer contains diaphosphorus pentoxide in an amount greater than or equal to 44 %.
[0032] Preferably, in the step S3, the moisture content is determined by the drying method, the plant protein is determined by the Kjeldahl method using a full-automatic nitrogen determination instrument, the plant ash is determined by using a muffle furnace, the plant fat is determined by using a fat instrument, the neutral detergent fiber is determined by using a FOSS Fibertec 2010 full-automatic fiber analysis system, and the acid detergent fiber is determined by using a FOSS Fibertec 2010 full-automatic fiber analysis system.
[0033] Preferably, in the step S4, the soil chemical property indexes comprise soil total nitrogen, total phosphorus, available potassium, and organic matter content.
[0034] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the soil bacterial community species composition in the soil sample of the Horqin degraded typical grassland.
[0035] Preferably, in the step S4, the high-throughput sequencing technology is used to analyze the soil fungal community species composition in the soil sample of the Horqin degraded typical grassland. BRIEF DESCRIPTION OF DRAWINGS Figure 1 is the average plant height in 2019 in experiment 5; Figure 2 is the average plant height in 2020 in experiment 5.
[0036] Experimental section Experiment 1 Influence of supplemental seeding on plant coverage of moderately degraded grassland
[0037] In June 2017 and June 2018, supplemental seeding treatment was carried out in the moderately degraded Kexi grassland, and one (A), two (B), three (C) and a control group were supplemented, as shown in Table 1 below. In July 2018, 180 kg / hm of nitrogen and phosphorus mixed fertilizer was applied 2 , the nitrogen and phosphorus mixed fertilizer contains nitrogen: phosphorus = 1:1, the nitrogen fertilizer is urea, the phosphorus fertilizer is heavy superphosphate, the urea contains nitrogen ≥ 46 %, the phosphorus fertilizer contains phosphorus pentoxide ≥ 44 %, the potassium fertilizer contains potassium oxide ≥ 51 %, the surrounding enclosure is not disturbed by human beings, and the investigation is carried out in the third and fourth years (2019, 2020), and the results are shown in Table 2.
[0038] Table 1 Design of supplemental seeding groups
[0039] Plant coverage refers to the percentage of the vertical projection area of the aboveground part of the plant population or community to the sampling area, which reflects the area of the vegetation for photosynthesis and its density. Coverage has plant base cover and projection cover (whole plant cover) two kinds, this experiment selects projection cover, that is, the percentage of the vertical projection area of the plant stem and leaf to the sample area, the average value is obtained by visual method; In June 2017 and June 2018, the surrounding enclosure is not disturbed by human beings after supplemental seeding, and the plant coverage is recorded, and the results are shown in Table 2.
[0040] Table 2 Influence of supplemental seeding on plant coverage of different functional groups
[0041] As can be seen from Table 2, the moderately degraded grassland sample plot (group A) supplemented by the present application has a decrease in one-year and two-year plant coverage in the third year (2019), and the perennial grasses, forbs and perennial legume plant cover are all higher than those of other groups, and are higher than those of other groups in the fourth year (2020). The fourth year (2020) is obviously better than the third year.
[0042] Experiment 2 Influence of supplemental seeding on plant density of different functional groups in moderately degraded grassland
[0043] The sample plot of experiment 1 was investigated for the influence of supplemental seeding on plant density in the third and fourth years (2019, 2020), and the results are shown in Table 3.
[0044] Table 3 Influence of supplemental seeding on plant density of different functional groups
[0045] Experiment 3 Influence of supplemental seeding on soil pH value For the sample plot of Experiment 1, the soil pH values of the 0-20 cm soil layer, 20-30 cm soil layer, and 30-50 cm soil layer were measured in 2019 and 2020, respectively, as shown in Table 4.
[0046] Table 4. Effects of reseeding on soil pH in different soil layers
[0047] As can be seen from the table above, the pH value of the moderately degraded Hormi grassland plot (Group A) that was reseeded with the present invention was lower than that of other groups in the third year (2019), which was an improvement compared to the alkalinity of other groups. In the fourth year (2020), the soil pH was close to neutral.
[0048] Experiment 4: Effects of reseeding on available phosphorus content in soil The available potassium content in the soil of the 0-20 cm, 20-30 cm, and 30-50 cm soil layers of the sample plot in Experiment 1 is shown in Table 5.
[0049] Table 5. Effects of reseeding on available potassium content in different soil layers
[0050] As can be seen from the table above, in the third year (2019), the available phosphorus content of the moderately degraded Hormi grassland plot (Group A) that was reseeded by this invention was higher than that of other groups, and the available potassium content in the fourth year (2020) increased compared with the third year.
[0051] Experiment 5: Effects of fertilization on the average height of plants in different functional groups In June 2017 and June 2018, reseeding was carried out on the moderately degraded Hormi grassland. In July 2018, fertilizer packets of 130 kg / hm² were applied. 2 The fertilizer package contained a nitrogen-phosphorus compound fertilizer, as detailed in Table 6. In the table, the nitrogen fertilizer was urea, the phosphorus fertilizer was superphosphate, and the potassium fertilizer was potassium oxide. The urea contained ≥46% nitrogen, the phosphorus fertilizer contained ≥44% phosphorus pentoxide, and the potassium fertilizer contained ≥51% potassium oxide. The enclosure was subsequently left undisturbed. Surveys were conducted in the third and fourth years (2019 and 2020), and the results are shown below. Figure 1 , 2 ,from Figure 1 , 2 It can be seen that when the ratio of nitrogen to phosphorus in the fertilizer pack was adjusted or potassium fertilizer was added, the vegetation growth was not as good as that of the present invention (nitrogen: phosphorus = 1:1).
[0052] Table 6 Fertilizer group design
[0053] Experiment 6: Effects of fertilization on soil total nitrogen content Soil total nitrogen is an indicator that measures the total amount of organic and inorganic nitrogen in soil. It mainly comes from the microbial decomposition process of soil organic matter. Its content can reflect the soil's potential nitrogen supply capacity to plants. In 2020, the effects of different fertilization methods on soil total nitrogen levels were statistically analyzed in Experiment 5, as shown in Table 7.
[0054] Table 7. Effects of different fertilization treatments on total nitrogen content in soil layers in 2020
[0055] As can be seen from Table 7, using the technical solution of the present invention, both nitrogen and phosphorus fertilizers and nitrogen and phosphorus fertilizer treatments can increase the total nitrogen content of the soil, and the effect of nitrogen:phosphorus = 1:1 is the best.
Claims
1. A method for ecological restoration of moderately degraded Horqin grassland, characterized in that, Includes the following steps: S1. Survey: Investigate the geographical overview, climate characteristics, vegetation overview, and soil overview of the Horqin Grassland, and identify the moderately degraded areas of the Horqin Grassland. S2. Plant community characteristics and biomass survey: During the peak growth period of pasture grass in July and August, a survey was conducted on the moderately degraded Horqin grassland plots to monitor the height, cover and density of the Horqin grassland plant community and collect data. S3. Determination of forage quality in plant communities: The quality of forage in moderately degraded Horqin grassland plant communities was determined by measuring moisture content, plant protein, plant ash, plant fat, neutral detergent fiber, and acid detergent fiber. S4. Soil sample collection: Soil samples were collected in August to determine soil chemical properties, soil bacterial community species composition, and soil fungal community species composition. S5. Reseeding: Reseed grass seeds are sown on the moderately degraded Horqin grassland. The reseeded grass seeds are *Agrostis pilosa* and *Agrostis spp.*, with a weight ratio of *Agrostis pilosa*: *Agrostis spp.* = 2.5-3.5:
1. The reseeding rate is 30-50 kg / hm². 2 Sowing depth 1-2cm, with reseeding for two consecutive years; S6. Fertilization: Fertilize in the second year after reseeding. Before rainfall, apply fertilizer packets of 150-210 kg / hm² manually. 2 The fertilizer package is a nitrogen-phosphorus mixed fertilizer, which includes nitrogen fertilizer and phosphorus fertilizer, wherein the ratio of nitrogen to phosphorus in the nitrogen-phosphorus mixed fertilizer is 1:
1.
2. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, The reseeded grass seeds were *Isodon spp.* and *Agrostis spp.*, with a quality ratio of *Isodon spp.* to *Agrostis spp.* = 3:
1.
3. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, The reseeded grass seeds were *Isodon spp.* and *Agrostis spp.*, with a weight ratio of *Isodon spp.* to *Agrostis spp.* = 2.5:
1.
4. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, The reseeded grass seeds were *Agrostis pilosa* and *Agrostis spp.*, with a quality ratio of *Agrostis pilosa*: *Agrostis spp.* = 3.5:
1.
5. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, The nitrogen fertilizer is urea, and the phosphate fertilizer is superphosphate.
6. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 5, characterized in that, The nitrogen content of the urea is ≥46%, and the phosphorus fertilizer contains ≥44% phosphorus pentoxide.
7. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, In step S3, the moisture content is determined by drying method, the plant protein is determined by Kjeldahl method using fully automated nitrogen analyzer, the plant ash is determined by muffle furnace, the plant fat is determined by fat analyzer, the neutral detergent fiber is determined by FOSS Fibertec 2010 fully automated fiber analysis system, and the acid detergent fiber is determined by FOSS Fibertec 2010 fully automated fiber analysis system.
8. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, In step S4, the soil chemical characteristics indicators include the content of total nitrogen, total phosphorus, available potassium, and organic matter.
9. The method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, In step S4, high-throughput sequencing technology is used to analyze the species composition of soil bacterial communities in soil samples from typical degraded grasslands in Horqin.
10. A method for ecological restoration of moderately degraded Horqin grassland as described in claim 1, characterized in that, In step S4, high-throughput sequencing technology is used to analyze the species composition of soil fungal communities in soil samples from typical degraded grasslands in Horqin.