Cell sap subpackaging device and subpackaging method

Abstract

The invention provides a cell sap subpackaging device and method, and aims to solve the problem of cell sap waste caused by pipeline residues and a calibration process in the prior art. The calibration liquid after flow calibration of the peristaltic pump is sucked back into the uniform mixing bag, and the steps of air emptying, pipeline filling, sensor calibration and the like are designed in the sub-packaging process, so that the residual cell sap can be effectively reduced, and the utilization rate of the cell sap is improved. The automatic subpackaging device is suitable for automatic subpackaging of high-value cell samples.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a device and method for cell liquid dispensing. BACKGROUND

[0002] In the field of biopharmaceuticals, cell therapy and life science research, the precise dispensing of cell liquid is a critical step in experimental operations and production processes. High-activity, high-value cell suspensions often need to be precisely dispensed into multiple containers (such as cryogenic tubes, culture dishes or reagent bottles) to ensure consistency and effectiveness in subsequent experiments or treatments. Currently, automated or semi-automated cell liquid dispensing systems have been widely used, and their core usually includes a liquid storage device, a liquid path pipeline, a driving mechanism (such as a peristaltic pump), and a dispensing execution mechanism.

[0003] In the prior art, the mainstream cell liquid dispensing system uses disposable pipeline consumables in conjunction with a peristaltic pump for cell liquid delivery. The specific operation process usually includes: installing the disposable pipeline to the peristaltic pump, and achieving directional movement of the cell liquid by rotating and extruding the pump; before formal dispensing, system calibration is required, i.e. using calibration liquid or part of the cell liquid to be dispensed to rinse and calibrate the system to ensure dispensing accuracy. After the calibration process is completed, the formal dispensing operation is performed.

[0004] However, the existing scheme has the following significant defects, resulting in serious waste of valuable cell liquid resources: 1. Serious internal residue of pipeline consumables: Because cell liquid has a certain adhesion, and the disposable pipeline has a long and complex internal diameter (often containing multiple joints, valves or three-way valves), a large amount of cell liquid will be left in the pipeline wall and dead space after dispensing. This part of the residual liquid cannot be effectively discharged and utilized, and is directly discarded with the consumables, causing loss of high-value cell resources.

[0005] 2. Calibration process causes a lot of waste: During the calibration of the peristaltic pump, a certain volume of cell liquid (or calibration liquid) is required to fill the pipeline, remove air bubbles and verify flow accuracy. This part of the cell liquid used for calibration is usually considered "contaminated" or "non-standard" cell liquid after calibration and cannot be used for formal dispensing, and must be discarded. For precious clinical or research cell samples, this "one-time calibration" mode leads to unnecessary and avoidable waste.

[0006] 3. High overall cost: The above waste and calibration not only directly lose the value of the cells themselves, but also increase the operating cost due to the need to frequently replace disposable consumables. At the same time, in order to reduce the residue, the existing scheme may use a more complex purging or flushing process, further increasing the operation complexity and consumption of additional reagents. Therefore, there is an urgent need to provide a new type of liquid route scheme, which can effectively reduce the residue in the pipeline consumables during the cell liquid dispensing process, and optimize or avoid the waste of cell liquid caused by the calibration link, significantly improve the utilization rate of cell liquid, reduce the experimental or production cost, especially suitable for the processing scene of high-value cell samples. SUMMARY

[0007] In order to solve the above technical defects, the present application provides a cell liquid dispensing method and device which can avoid the serious waste of valuable cell liquid resources.

[0008] A cell liquid dispensing method, characterized in that it comprises the following steps: S1 obtaining the mixing capacity of the mixing bag and the mixing cell type; S2 determining the number of cell liquid bags, the corresponding cell type, and the capacity of the cell liquid bag; S3 type confirmation of the mixing cell type and the dispensing cell type is performed to obtain the type confirmation result corresponding to each dispensing bag, and capacity confirmation of the mixing capacity and each dispensing capacity is performed to obtain the capacity confirmation result corresponding to each dispensing bag; S4 pre-evacuation of the mixing bag is performed to discharge the air in the mixing bag; the cell liquid in the cell liquid bag is transferred to the mixing bag through the driving of the first peristaltic pump 401; cell mixing is performed; and the cell liquid in the mixing bag is dispensed into the cryopreservation bag through the driving of the second peristaltic pump 402.

[0009] The dispensing cell type refers to the type of cells that need to be dispensed into the cryopreservation bag. After confirming the type of cells to be dispensed, the mixing parameters required can be determined.

[0010] Further, step S1 is to select the mixing bag capacity and cell liquid type through the PC software interface.

[0011] Further, before performing the driving operation, the first peristaltic pump performs calibration and back suction in the following manner: the first peristaltic pump is rotated forward to complete the calibration of the first peristaltic pump, and then the first peristaltic pump is rotated in reverse to suck the cell liquid back into the cell liquid bag.

[0012] Further, the specific steps of dispensing the cell liquid into the cryopreservation bag are: connecting the cell liquid bag and the mixing bag; and the second peristaltic pump is rotated forward to fill the cell liquid into the mixing bag.

[0013] Further, after the second peristaltic pump flow calibration is completed and before the cryogenic bag is filled, the air in the cryogenic bag is evacuated in the following manner: the second peristaltic pump is controlled to rotate in reverse, air in the cryogenic bag is pumped out, and when the pressure in the cryogenic bag reaches a threshold value as detected by the pressure sensor, the second peristaltic pump stops rotating in reverse.

[0014] Further, after the second peristaltic pump calibration is completed and before the cell solution is dispensed into the cryogenic bag, all pinch valves at the entrances of the cryogenic bags are closed, and the pipeline is filled in the following manner: the second peristaltic pump is controlled to rotate in the forward direction, so that the cell solution flows from the second peristaltic pump into the dispensing pipeline, and when the pressure in the dispensing pipeline reaches a threshold value, the second peristaltic pump stops rotating.

[0015] Further, after the pipeline is filled, the filling of each cryogenic bag is started one by one.

[0016] Further, after a cryogenic bag is filled, the air in the cryogenic bag is evacuated in the following manner: the second peristaltic pump is controlled to rotate in reverse, air in the cryogenic bag is pumped out, and when the pressure in the cryogenic bag reaches a threshold value as detected by the pressure sensor, the second peristaltic pump stops rotating in reverse.

[0017] A cell solution dispensing device comprises the following structure: a first cell solution bag is connected to a first pinch valve, a second cell solution bag is connected to a second pinch valve, a first filter is connected to a fifth pinch valve, the first pinch valve, the second pinch valve, and the fifth pinch valve are all connected in parallel to one end of a first peristaltic pump, the other end of the first peristaltic pump is connected in parallel to two liquid paths: one of which is connected in sequence to a third pinch valve, a first calibration sensor J01, and a second filter, starting from the first peristaltic pump; the other of which is connected in sequence to a fourth pinch valve, a first bubble detection device, a first pressure sensor, and one end of a mixing bag, starting from the first peristaltic pump; the other end of the mixing bag is connected to one or multiple filling liquid paths, wherein the first filling liquid path is connected in sequence to a sixth pinch valve, starting from the mixing bag, the other end of the sixth pinch valve is connected in parallel to a seventh pinch valve and a second peristaltic pump, the other end of the seventh pinch valve is connected in sequence to a buffer bag and a third filter, the other end of the second peristaltic pump is connected in sequence to an eighth pinch valve, a second calibration sensor, a second pressure sensor, and one end of a second bubble detection device; each of multiple cryogenic bags is connected to a cryogenic bag pinch valve; the cryogenic bag pinch valves are connected in parallel to the other end of the second bubble detection device; a one-way valve is also connected in parallel to the other end of the second bubble detection device through a fourth filter.

[0018] Further, the first calibration sensor and the second calibration sensor are both ultrasonic flow sensors.

[0019] Furthermore, compared to traditional flow calibration bottles, the flow sensor is directly fixed to the pipeline, eliminating the need for a locally enlarged cavity in the traditional calibration bottle. This avoids the problem of liquid residue inside the calibration bottle and greatly reduces cell fluid waste. In addition, compared to traditional peristaltic pump calibration, it eliminates the need for inlet and outlet sensors at both ends of the calibration bottle, reducing costs and the complexity of software operation.

[0020] This invention uses the reverse rotation of a peristaltic pump after flow calibration to draw the calibration solution back into the mixing bag. It also incorporates steps such as air evacuation and tubing filling in the dispensing process, which effectively reduces cell fluid residue and improves cell fluid utilization. This invention is suitable for automated dispensing of high-value cell samples. Attached Figure Description

[0021] Figure 1 This is a flowchart of the process from cell sap bag to mixing bag provided in an embodiment of the present invention.

[0022] Figure 2 This is a flowchart of the operation from mixing bag to dispensing to cryopreservation bag provided by an embodiment of the present invention.

[0023] Figure 3 This is a schematic diagram of the liquid path structure of the cell fluid dispensing device according to an embodiment of the present invention.

[0024] Reference numerals: 101, First cell culture bag; 102, Second cell culture bag; 2, Mixing bag; 3, Cryopreservation bag; 401, First peristaltic pump; 402, Second peristaltic pump; 501, First pressure sensor; 502, Second pressure sensor; 6, One-way valve; H01, Buffer bag; F01, First clamp valve; F02, Second clamp valve; F03, Third clamp valve; F04, Fourth clamp valve; F05, Fifth clamp valve; F06, Sixth clamp valve; F07, Seventh clamp valve; F08, Eighth clamp valve; Fn, Cryopreservation bag clamp valve; L01, First filter; L02, Second filter; L03, Third filter; L04, Fourth filter; Q01, First bubble detection device; Q02, Second bubble detection device; J01, First calibration sensor; J02, Second calibration sensor. Detailed Implementation

[0025] This invention provides a cell fluid dispensing device, which includes: a first cell fluid bag 101, a second cell fluid bag 102, a mixing bag 2, a cryopreservation bag 3, a first peristaltic pump 401, a second peristaltic pump 402, a first pressure sensor 501, a second pressure sensor 502, a one-way valve 6, a buffer pack H01, a first clamp valve F01, a second clamp valve F02, a third clamp valve F03, a fifth clamp valve F05, a sixth clamp valve F06, a seventh clamp valve F07, an eighth clamp valve F08, a cryopreservation bag clamp valve Fn, a first filter L01, a second filter L02, a third filter L03, a fourth filter L04, a first bubble detection device Q01, a second bubble detection device Q02, a first calibration sensor J01, and a second calibration sensor J02. The above components are connected by consumable tubing, tee fittings, and other pipe joints to form a liquid circuit.

[0026] like Figure 3 As shown, the first cell fluid bag 101 is connected to the first clamp valve F01, the second cell fluid bag 102 is connected to the second clamp valve F02, and the first filter L01 is connected to the fifth clamp valve F05. The first clamp valve F01, the second clamp valve F02, and the fifth clamp valve F05 are connected in parallel to one end of the first peristaltic pump 401. The other end of the first peristaltic pump 401 is connected to two liquid paths in parallel: one path starts from the first peristaltic pump 401 and is connected in sequence to the third clamp valve F03, the first calibration sensor J01, and the second filter L02; the other path starts from the first peristaltic pump 401 and is connected in sequence to the fourth clamp valve F04, the first bubble detection device Q01, the first pressure sensor 501, and the mixing bag 2.

[0027] like Figure 3 As shown, the other end of the mixing bag 2 can be connected in parallel to multiple liquid filling lines, such as... Figure 3 The embodiment shown illustrates the case of two parallel liquid filling circuits. Among them: (1) The first liquid filling circuit starts from the mixing bag 2 and is connected to the sixth clamp valve F06. The other end of the sixth clamp valve F06 is connected in parallel to the seventh clamp valve F07 and the second peristaltic pump 402. The other end of the seventh clamp valve F07 is connected in sequence to the buffer pack H01 and the third filter L03. The other end of the second peristaltic pump 402 is connected in sequence to the eighth clamp valve F08, the second calibration sensor J02, the second pressure sensor 502, and the second bubble detection device Q02. Each of the eight cryopreservation bags 3 is connected in parallel to the other end of the second bubble detection device Q02 after passing through a cryopreservation bag clamp valve Fn. The one-way valve 6 is also connected in parallel to the other end of the second bubble detection device Q02 after passing through the fourth filter L04, so that the other end of the second bubble detection device Q02 forms a nine-way parallel structure. (2) After the second liquid filling channel starts from the mixing bag 2, the structure is the same as that of the first liquid filling channel, or the number and / or capacity of the cryopreservation bags in the second liquid filling channel are allowed to be different from those in the first liquid filling channel.

[0028] likeFigure 3 As shown, the specific liquid path control of the cell solution dispensing device is as follows when the cell solution is transferred from the mixing bag to the cryopreservation bag.

[0029] (1) Pre-emptive venting: Connect the required consumable pipelines, select the mixing bag capacity, and click "Start" to close the first clamp valve F01, the second clamp valve F02, and the third clamp valve F03, and open the fourth clamp valve F04 and the fifth clamp valve F05. The first peristaltic pump 401 rotates in reverse to extract the gas from the mixing bag 2 and discharge it to the outside through the first filter L01. The first filter L01 ensures that the pipeline is in a sterile environment, preventing external contamination of the pipeline. Detect the value of the first pressure sensor 501. After reaching the corresponding threshold, the first peristaltic pump 401 stops rotating. Close the fourth clamp valve F04 and the fifth clamp valve F05.

[0030] (2) Calibration: After completing the above evacuation operation, open the first clamp valve F01 and the third clamp valve F03 to make the first peristaltic pump 401 rotate in the forward direction. The volume of liquid flowing through the sensor is obtained by detecting the first calibration sensor J01, thereby completing the flow calibration of the first peristaltic pump 401. After calibration, make the first peristaltic pump 401 rotate in the reverse direction to draw the cell fluid back into the first cell fluid bag 101 until the flow sensor detects that the amount of liquid drawn back is the same as the amount of liquid drawn forward, and the first peristaltic pump 401 stops moving.

[0031] (3) Infusion: After completing the above calibration, connect the first clamp valve F01 and the fourth clamp valve F04, and the first peristaltic pump 401 rotates in the forward direction to transfer the cell fluid in the first cell fluid bag 101 to the mixing bag 2. After the transfer in the first cell fluid bag 101 is completed, close the first clamp valve F01 and open the second clamp valve F02 to transfer the cell fluid in the second cell fluid bag 102. After the transfer in the second cell fluid bag 102 is completed, close all clamp valves.

[0032] (4) Mixing: Start cooling and mixing so that the cell solution in mixing bag 2 is mixed in a low temperature environment of 2-8℃.

[0033] This invention supports multiple parallel filling channels connected to the mixing bag for dispensing, and can simultaneously control the peristaltic pump on each filling channel for dispensing. The following is a detailed description of one of the filling channel solutions. Figure 3 As shown, the specific liquid path control of the cell solution dispensing device is as follows when the cell solution is transferred from the mixing bag to the cryopreservation bag.

[0034] (1) Pre-emptive venting: After cooling and mixing, start the filling of cryopreservation bags. First, close the sixth clamp valve F06, and at the same time open the seventh clamp valve F07 and the eighth clamp valve F08. Control the first of the multiple cryopreservation bag clamp valves Fn to open, while keeping the others closed. The second peristaltic pump 402 rotates in the opposite direction to vent the gas in the cryopreservation bag 3 connected to the first cryopreservation bag clamp valve. At the same time, detect the second pressure sensor 502. When the sensor reaches the threshold, close the first cryopreservation bag clamp valve Fn. Then, follow the above venting method to complete the pre-emptive venting action of the remaining cryopreservation bags 3 in sequence.

[0035] (2) Calibration: Open the sixth clamp valve F06 and the eighth clamp valve F08, and close the seventh clamp valve F07. The second peristaltic pump 402 rotates in the forward direction. The volume of liquid flowing through the sensor is obtained by detecting the second calibration sensor J02, thereby completing the flow calibration of the second peristaltic pump 402. After calibration, the second peristaltic pump 402 rotates in the reverse direction to draw the cell fluid back into the mixing bag 2 until the flow sensor detects that the amount of liquid drawn back is the same as the amount of liquid drawn forward. The second peristaltic pump 402 then stops moving, completing the flow calibration.

[0036] (3) Pipeline filling: After calibration, open the sixth clamp valve F06 and the eighth clamp valve F08, close the seventh clamp valve F07, and close all the clamp valves Fn of the cryopreservation bags; the second peristaltic pump 402 rotates in the forward direction to carry out pipeline filling work. During the filling process, the second pressure sensor 502 is detected. After the set threshold is reached, the second peristaltic pump 402 stops rotating, and the pipeline filling task is completed.

[0037] (4) Filling of cryopreservation bags: After the pipeline filling is completed, open the first cryopreservation bag clamp valve Fn, and the second peristaltic pump 402 rotates in the forward direction. When the first bubble detection device Q01 detects the cell fluid, it starts to measure the liquid volume and fills the cryopreservation bag connected to the first cryopreservation bag clamp valve Fn.

[0038] (5) Post-filling: After the cryopreservation bag connected to the first cryopreservation bag clamp valve Fn is filled, the second peristaltic pump 402 rotates in the reverse direction to perform the post-filling venting action of the filled cryopreservation bag. When the second bubble detection device Q02 detects bubbles and then detects liquid, the second peristaltic pump 402 rotates in the forward direction to transport the cell fluid in the pipeline into the cryopreservation bag, thus completing the filling of one cryopreservation bag. Other cryopreservation bags are filled in the same way as described above until all cryopreservation bags are filled.

[0039] The buffer pack H01 is in an open state during the exhaust and filling process, ensuring that trace amounts of liquid will not wet the third filter L03 when air is being introduced or removed, thus avoiding pipeline blockage.

[0040] The second bubble detection device Q02 should be installed as close as possible to the cryopreservation bag. This will reduce the impact of pipeline errors on the peristaltic pump's accuracy during the back-pushing process after the exhaust gas is released.

[0041] The peristaltic pump flow calibration sensor of this invention uses an ultrasonic flow sensor, which increases the flow rate and improves the calibration accuracy of the peristaltic pump. After the cell fluid in the final mixing bag 2 is emptied, the remaining cells can be pressed into the cryopreservation bag 3 by the forward rotation of the peristaltic pump with the help of air pressure, thus reducing the waste of cell fluid.

[0042] This invention provides a dispensing method using the above-described cell fluid dispensing device, the method comprising the following steps: S1: Obtain the mixing capacity of the mixing bag and the type of cells to be mixed; S2: Determine the number, type, and capacity of cell sap bags; S3: Manually confirm the mixed cell type and each packaged cell type by selecting the corresponding option through the operation software interface to obtain the type confirmation result corresponding to each packaged bag, and confirm the capacity of each packaged bag to obtain the capacity confirmation result corresponding to each packaged bag. S4: After receiving the dispensing order, according to the dispensing order, and the obtained cell types and capacities of each dispensing unit; according to... Figure 3 The liquid flow diagram describes the process of transferring the cell sap from the cell sap bag to the mixing bag for cell mixing, and then dispensing the mixture from the mixing bag into cryopreservation bags after mixing.

[0043] In step S1, when obtaining the mixing capacity and cell fluid type of the mixing bag, you can choose to select the mixing bag capacity and cell fluid type through the PC software interface.

[0044] In step S4, the mixing bag is first evacuated by controlling the clamp valve. After evacuation, the clamp valve connects the cell solution bag to the flow calibration sensor, and the peristaltic pump rotates forward to detect air bubbles and calibrate the flow rate. After calibration, the peristaltic pump rotates in reverse to reabsorb the cell solution. Then, the cryopreservation bag filling begins. After purging the gas from the cryopreservation bag, the flow pump is calibrated, and the clamp valve connects the cell solution bag to the mixing bag. The peristaltic pump rotates forward to fill the mixing bag with the cell solution. After filling, the clamp valve connects the filter to the mixing bag, and the peristaltic pump rotates in reverse to mix the air in the bag before purging.

[0045] Specifically, such as Figure 1As shown, the process of transferring cell fluid from the cell fluid bag to the mixing bag is as follows: (1) Pre-emptive purging: Control the clamp valve and rotate the first peristaltic pump 401 to purge the gas in the mixing bag 2. (2) Calibration: Control the clamp valve and extract the cell fluid from the cell fluid bag, which flows through the first calibration sensor J01 to perform flow rate calibration of the first peristaltic pump 401. After the flow rate calibration is completed, the peristaltic pump rotates in the reverse direction to draw back the cell fluid. (3) Infusion: Hang the cell fluid bags properly and control the clamp valve to extract the cell fluid from the cell fluid bags and infuse it into the mixing bag. (4) Mixing: Mix the cell fluid in the mixing bag in a low-temperature environment of 2-8℃.

[0046] like Figure 2 As shown, the process of cell fluid from the mixing bag to the cryopreservation bag is as follows: (1) Pre-emptive evacuation: Control the clamp valve, detect the pressure sensor, and extract the gas from the cryopreservation bag. (2) Calibration: Control the clamp valve, extract the cell fluid from the mixing bag, and flow through the second calibration sensor J02 to perform flow calibration of the second peristaltic pump 402. (3) Pipeline filling: Control the clamp valve, open the peristaltic pump, and detect the pressure sensor to perform pipeline filling. (4) Cryopreservation bag filling: Control the clamp valve, open the peristaltic pump to fill the cryopreservation bag. (5) Post-emptive evacuation: Control the clamp valve, open the peristaltic pump, and remove air bubbles from the cryopreservation bag.

[0047] like Figure 3 As shown, the flow calibration sensor in the process uses an ultrasonic flow sensor to ensure that the final cell solution is completely delivered to the cryopreservation bag while calibrating the flow rate, thus avoiding waste of the cell solution.

[0048] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for dispensing cell fluid, comprising transferring cell fluid from a cell fluid bag to a mixing bag for mixing, and then dispensing it into multiple cryopreservation bags, characterized in that, Includes the following steps: S1 obtains the mixing capacity of the mixing bag and the type of cells to be mixed; S2 determines the number of cell sap bags, the corresponding cell types, and the capacity of the cell sap bags; S3. Confirm the type of the mixed cell and the type of the dispensed cell to obtain the type confirmation result corresponding to each dispensed bag. Confirm the capacity of the mixed capacity and the capacity of each dispensed bag to obtain the capacity confirmation result corresponding to each dispensed bag. S4 performs a pre-emptive venting of the mixing bag to remove air from inside the bag; The cell sap in the cell sap bag is transferred to the mixing bag by the drive of the first peristaltic pump (401); Mix the cells thoroughly; The cell solution in the mixing bag is dispensed into the cryopreservation bag by the drive of the second peristaltic pump (402).

2. The cell fluid dispensing method according to claim 1, characterized in that: Step S1 involves selecting the mixing bag volume and cell culture type via the PC software interface.

3. The cell fluid dispensing method according to claim 1, characterized in that, Before performing the driving operation, the first peristaltic pump (401) is calibrated and re-absorbed in the following manner: the first peristaltic pump (401) is rotated in the forward direction to complete the calibration of the first peristaltic pump (401), and then the first peristaltic pump (401) is rotated in the reverse direction to re-absorb the cell fluid into the cell fluid bag.

4. The cell fluid dispensing method according to claim 3, characterized in that, The specific steps for dispensing the cell solution into the cryopreservation bag are as follows: connecting the cell solution bag and the mixing bag; the second peristaltic pump (402) rotates in the forward direction to fill the cell solution into the mixing bag.

5. The cell fluid dispensing method according to claim 4, characterized in that, After the flow rate calibration of the second peristaltic pump (402) is completed and before the cryopreservation bag is filled, the air in the cryopreservation bag is purged in the following manner: the second peristaltic pump (402) is controlled to rotate in reverse to evacuate the gas in the cryopreservation bag. After the pressure in the cryopreservation bag is confirmed to have reached the threshold by the pressure sensor, the second peristaltic pump (402) stops rotating in reverse.

6. The cell fluid dispensing method according to claim 5, characterized in that, After the second peristaltic pump (402) is calibrated and before the cell solution is dispensed into the cryopreservation bags, close all clamp valves (Fn) at the inlet of the cryopreservation bags and fill the tubing as follows: the second peristaltic pump (402) rotates in the forward direction so that the cell solution enters the dispensing tubing from the second peristaltic pump (402). After the pressure in the dispensing tubing reaches the threshold, the second peristaltic pump (402) stops rotating.

7. The cell fluid dispensing method according to claim 6, characterized in that: After the pipeline is filled, the filling process for each cryopreservation bag is started one by one.

8. The cell fluid dispensing method according to claim 7, characterized in that: After a cryopreservation bag is filled, the gas inside the cryopreservation bag is purged as follows: the second peristaltic pump (402) is rotated in the reverse direction to extract air from the cryopreservation bag; the pressure value of the pressure sensor on the pipeline is detected to confirm that the gas inside the cryopreservation bag has been purged; the second peristaltic pump (402) is rotated in the forward direction to transport the cell fluid in the pipeline between the pressure sensor and the cryopreservation bag back into the cryopreservation bag.

9. A cell fluid dispensing device, characterized in that, The structure includes the following: a first cell fluid bag (101) is connected to a first clamp valve (F01), a second cell fluid bag (102) is connected to a second clamp valve (F02), a first filter (L01) is connected to a fifth clamp valve (F05), and the first clamp valve (F01), the second clamp valve (F02) and the fifth clamp valve (F05) are all connected in parallel to one end of a first peristaltic pump (401). The other end of the first peristaltic pump (401) is connected in parallel to two liquid circuits: one circuit starts from the first peristaltic pump (401) and is connected in sequence to a third clamp valve (F03), a first calibration sensor (J01) and a second filter (L02); the other circuit starts from the first peristaltic pump (401) and is connected in sequence to a fourth clamp valve (F04), a first bubble detection device (Q01), a first pressure sensor (501) and one end of a mixing bag (2). The other end of the mixing bag (2) is connected to one or more canned liquid circuits. The first canned liquid circuit starts from the mixing bag (2) and is connected to the sixth clamp valve (F06). The other end of the sixth clamp valve (F06) is connected in parallel to the seventh clamp valve (F07) and the second peristaltic pump (402). The other end of the seventh clamp valve (F07) is connected in sequence to the buffer pack (H01) and the third filter (L03). The other end of the second peristaltic pump (402) is connected in sequence to the eighth clamp valve (F08), the second calibration sensor (J02), the second pressure sensor (502), and one end of the second bubble detection device (Q02). Each of the multiple cryopreservation bags (3) is connected to a cryopreservation bag clamp valve (Fn). The cryopreservation bag clamp valve is connected in parallel to the other end of the second bubble detection device (Q02). The one-way valve (6) passes through the fourth filter (L04) and is also connected in parallel with the cryopreservation bag clamp valve (Fn) to the other end of the second bubble detection device (Q02).

10. The cell fluid dispensing device according to claim 9, characterized in that: Both the first calibration sensor (J01) and the second calibration sensor (J02) are ultrasonic flow sensors.

11. The cell fluid dispensing apparatus according to claim 10, characterized in that: The ultrasonic flow sensor calculates the flow velocity of the fluid by measuring the time difference, frequency difference, and / or phase difference of ultrasonic waves propagating in the fluid in the direction of flow and against the flow, thereby calculating the volumetric flow rate.

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