Application of choline chloride and aspartic acid compound in increasing wheat yield

Abstract

The invention belongs to the technical field of plant growth promoters, and particularly relates to application of a choline chloride and aspartic acid compound to increase of wheat yield. The mass ratio of choline chloride to aspartic acid is (0.5-1.5): (0.5-1.5). Field trials prove that the yield of wheat can be remarkably increased by using the choline chloride and aspartic acid solution for foliage spraying in the elongation stage of wheat.

Description

Technical Field

[0001] This invention belongs to the technical field of plant growth promoters, specifically relating to the application of a compound of choline chloride and aspartic acid in increasing wheat yield. Background Technology

[0002] As a crucial food crop in my country, wheat grain filling is the core stage of grain weight formation and plays a decisive role in yield. In modern high-yield wheat cultivation systems, optimizing grain filling through agronomic measures is essential. As the growth period progresses, wheat biomass increases rapidly, making topdressing more difficult and significantly depleting soil nutrients. Foliar fertilization, due to its ease of application and ability to precisely supply the nutrients needed for wheat growth and development during the grain filling stage, has become an important approach to improving grain filling.

[0003] Choline chloride (CC), a typical choline derivative, is composed of choline cations and chloride ions and is an important metabolic regulator for plant growth and development. Exogenous choline chloride can be metabolized into betaine and phosphatidylcholine. The former enhances plant stress resistance by reducing membrane lipid peroxidation and regulating osmotic pressure, while the latter maintains membrane stability by binding water molecules with polar groups. In agricultural applications, foliar spraying of choline chloride can scavenge reactive oxygen species (ROS), reduce lipid peroxidation under salt stress, and promote photosynthesis and cytokinin synthesis, thereby improving crop salt tolerance and productivity. Aspartic acid is a key amino acid in plant metabolism. It is not only a precursor for the synthesis of other amino acids and proteins but also interacts with plant hormones to regulate plant growth and development. As a basic amino acid, it participates in the synthesis of other amino acids, nucleotides, and other substances, and is crucial for plant development and stress resistance. As an exogenous substance, aspartic acid can act as a micronutrient or growth promoter, positively impacting crop growth and yield.

[0004] Currently, although some studies have explored the functions of choline and its analogues, there are no reports on the role of choline in combination with amino acids in regulating wheat growth and yield. Summary of the Invention

[0005] To address the aforementioned technical issues, this invention provides an application of a combination of choline chloride and aspartic acid in increasing wheat yield. Field trials have verified that foliar spraying with a choline chloride + aspartic acid solution (each with a mass concentration of 50-200 mg / L, resulting in a total concentration of 100-400 mg / L after mixing) at the wheat jointing stage significantly increases wheat yield. This technique is simple to operate, low in cost, and uses low-toxicity or non-toxic compounds, making it highly valuable for widespread application.

[0006] The specific technical solution provided by this invention is as follows: This invention provides an application of a compound of choline chloride and aspartic acid in improving wheat yield, wherein the mass ratio of choline chloride to aspartic acid is 0.5~1.5:0.5~1.5.

[0007] In a preferred embodiment of the present invention, the mass ratio of choline chloride to aspartic acid is 1:1.

[0008] In a preferred embodiment of the present invention, the choline chloride and the aspartic acid are combined to regulate the grain filling process of wheat.

[0009] More preferably, the choline chloride and the aspartic acid are combined to prolong the effective grouting time and increase the average grouting rate and active grouting period.

[0010] In a preferred embodiment of the present invention, the choline chloride and aspartic acid are sprayed onto the leaves of wheat during the jointing stage.

[0011] More preferably, the mass concentrations of both choline chloride and aspartic acid during spraying are 50 mg / L to 200 mg / L.

[0012] More preferably, the mass concentration of both choline chloride and aspartic acid is 100 mg / L during spraying. Optimal yield can be obtained at this concentration.

[0013] The present invention also provides a reagent for increasing wheat yield, which uses a compound of choline chloride and aspartic acid as the active ingredient, wherein the mass ratio of choline chloride to aspartic acid is 0.5~1.5:0.5~1.5, and includes acceptable excipients. The excipients are selected from surfactants, stabilizers, antifreeze agents, preservatives, colorants, or synergists.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention is the first to discover that foliar spraying of a combination of choline chloride and aspartic acid significantly improves wheat yield. Field trials have verified that, compared to choline chloride alone, foliar spraying with choline chloride and aspartic acid significantly improves wheat grain filling and yield. This method is simple to operate, and both choline chloride and aspartic acid are commercially available and inexpensive reagents. Furthermore, all reagents used in this method are environmentally friendly. Choline chloride has extremely low toxicity and is commonly used as a feed additive, while aspartic acid is a non-toxic and environmentally friendly compound. Therefore, this method has high biosafety and environmental compatibility. Detailed Implementation

[0015] The specific embodiments of the present invention are described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods described in the embodiments of the present invention are conventional methods.

[0016] All concentration values ​​mentioned in this invention are based on the concentration of the active ingredient. For example, commercially available 60% choline chloride aqueous solution is prepared and used after being adjusted according to the active ingredient content of 100 mg / L.

[0017] In the later stages of wheat growth, root systems begin to age, the climate becomes drier, soil nutrients are depleted, plants age prematurely, and grain filling is insufficient, thus reducing wheat yield. Foliar nitrogen application is not only convenient to implement but also helps to quickly replenish nutrients during the wheat grain-filling period, which is of great significance for ensuring good growth in the later stages. Therefore, this invention provides an application of a compound of choline chloride and aspartic acid in increasing wheat yield, wherein the mass ratio of choline chloride to aspartic acid is 0.5~1.5:0.5~1.5.

[0018] This invention utilizes a foliar spray of a compound choline chloride and aspartic acid to delay wheat senescence and extend the grain-filling time, thereby increasing wheat yield and providing technical support for wheat production.

[0019] 1. Method Field trials and tests were conducted at the Agronomic Experiment Base of Northwest A&F University. During the wheat jointing stage, on a sunny, windless afternoon, a foliar spray of a choline chloride and aspartic acid compound reagent was applied. The mass ratio of choline chloride to aspartic acid was 1:1, and water was used as the solvent. Choline chloride and aspartic acid were prepared into solutions with different concentration gradients: choline chloride (50 mg / L, 100 mg / L, 200 mg / L) + aspartic acid (50 mg / L, 100 mg / L, 200 mg / L) solutions, mixed in equal volumes (labeled C+D). Choline chloride alone (100 mg / L) served as the control group (labeled C), and water served as the blank control group (labeled CK). The experiment employed a completely randomized design, with three replicates per treatment, and each replicate was randomly distributed in the field. Both choline chloride and aspartic acid were commercially available reagents, and both are low-toxicity compounds, inexpensive, and readily operable.

[0020] It should be noted that when the mass concentrations of both choline chloride and aspartic acid are between 50 mg / L and 200 mg / L, the expected effects of the present invention can be obtained when the mass ratio of choline chloride to aspartic acid is between 0.5 and 1.5: 0.5 and 1.5. The above-mentioned equal volume mixing (i.e., equal mass ratio mixing) of choline chloride and aspartic acid is not a special limitation on the present invention, but only an example of the technical solution of the present invention.

[0021] 2. Results 2.1 Effects of foliar spraying of a compound agent of choline chloride and aspartic acid at the jointing stage on wheat yield The results are shown in Table 1.

[0022] Table 1. Effects of the combination of choline chloride and aspartic acid on wheat yield. Note: C represents choline chloride, D represents aspartic acid, and different lowercase letters in the table indicate significant differences between treatments (P < 0.05).

[0023] As shown in Table 1, compared with choline chloride alone, spraying choline chloride + aspartic acid (C+D) at the jointing stage significantly increased wheat yield at all three compound concentrations, and the effect was better than using choline chloride alone. This indicates that the yield-increasing effect of choline chloride combined with aspartic acid is significantly higher than that of choline chloride alone.

[0024] 2.2 Effects of foliar spraying of a compound agent of choline chloride and aspartic acid at the jointing stage on wheat grain filling The results are shown in Tables 2-4.

[0025] Table 2. Logistic equation parameters for grain filling in winter wheat under the combined effects of choline chloride and aspartic acid. Note: C (choline chloride 100 mg / L), C+D (choline chloride 100 mg / L + aspartic acid 100 mg / L) Table 3. Effects of choline chloride and aspartic acid combination on grain filling characteristic parameters of winter wheat. Note: C (choline chloride 100 mg / L) C+D (choline chloride 100 mg / L + aspartic acid 100 mg / L), different lowercase letters indicate significant differences between treatments, P < 0.05. Tmax: time to maximum grouting rate (d); Gmax: maximum grouting rate (g / d); T0.99: effective grouting time (d); Gmean: average grouting rate (g / d); D: active grouting period (d).

[0026] Table 4. Effects of choline chloride and aspartic acid combination on characteristic parameters of winter wheat grains at three grain-filling stages. Note: T1: Duration of the gradual grouting growth period; G1: Average grouting rate during the gradual grouting growth period; T2: Duration of the rapid grouting growth period; G2: Average grouting rate during the rapid grouting growth period; T3: Duration of the slow grouting growth period; G3: Average grouting rate during the slow grouting growth period. C (choline chloride 100 mg / L) C+D (choline chloride 100 mg / L + aspartic acid 100 mg / L). Different lowercase letters indicate significant differences between treatments, P < 0.05.

[0027] Based on the analysis results in Tables 2, 3, and 4, choline chloride (C) alone and the combined treatment of choline chloride and aspartic acid (C+D) significantly regulate the grain filling process of winter wheat. From the perspective of Logistic equation parameters, both treatments improved the model fit and optimized the grain filling correlation coefficient; regarding grain filling characteristic parameters, they effectively prolonged the grain filling duration (T). 0.99 This improved the average grouting rate (G). mean The analysis of characteristic parameters of the three grouting stages (C+D) showed that they had a positive impact on the dry matter accumulation in the initiation, stabilization and decay stages of the grouting process. The C+D treatment performed better in most indicators, showing a synergistic effect.

[0028] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. If such modifications and variations fall within the scope of equivalents of this invention, then this invention also intends to include these modifications and variations.

Claims

1. An application of a compound of choline chloride and aspartic acid in increasing wheat yield, characterized in that, The mass ratio of choline chloride to aspartic acid is 0.5~1.5:0.5~1.

5.

2. The application according to claim 1, characterized in that, The mass ratio of choline chloride to aspartic acid is 1:

1.

3. The application according to claim 1, characterized in that, The combination of choline chloride and aspartic acid is used to regulate the grain filling process of wheat.

4. The application according to claim 3, characterized in that, The combination of choline chloride and aspartic acid is used to prolong the effective grouting time and increase the average grouting rate and active grouting period.

5. The application according to claim 1, characterized in that, In practical applications, the choline chloride and aspartic acid are sprayed onto the leaves of wheat during the jointing stage.

6. The application according to claim 5, characterized in that, When spraying, the mass concentrations of both choline chloride and aspartic acid are 50 mg / L to 200 mg / L.

7. The application according to claim 6, characterized in that, The mass concentration of both choline chloride and aspartic acid was 100 mg / L during spraying.

8. A reagent for increasing wheat yield, characterized in that, It uses a complex of choline chloride and aspartic acid as its active ingredient, wherein the mass ratio of choline chloride to aspartic acid is 0.5~1.5:0.5~1.5, and includes acceptable excipients.

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