Method for improving cutting rooting rate of acer palmatum

Abstract

The invention aims to provide a method for improving the cutting rooting rate of acer palmatum, which comprises the following steps: acquiring acer palmatum cutting slips, and disinfecting the acer palmatum cutting slips; treating bases of the cutting slips with rooting liquid; the rooting liquid contains ABT1 rooting powder with the final concentration of 100 to 200 mg / L, IBA with the final concentration of 150 to 200 mg / L and ascorbic acid with the final concentration of 25 to 100 mg / L; and performing cuttage and post-cuttage management on the cutting slips treated by the rooting liquid. By the adoption of the method, the highest rooting rate of acer palmatum twig cuttage can be stably increased to 29.6% from 18.5% at present, the rooting rate is remarkably increased by 11.1%, and an essential basis is provided for survival of acer palmatum.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and specifically to a method for improving the rooting rate of blood maple cuttings. Background Technology

[0002] Blood-bark maple ( Acer griseum Due to its unique flaky bark and beautiful tree shape, the blood-bark maple has extremely high ornamental value and is a precious landscaping tree species. Currently, the propagation of blood-bark maple mainly relies on seed sowing, but seeds have problems such as long dormancy period, low germination rate, and easy variation in offspring traits, making it difficult to meet the needs of large-scale production. Cutting propagation, as a form of asexual reproduction that can maintain the superior traits of the parent plant, has significant application potential.

[0003] However, *Acer rubrum* is a difficult-to-root species, especially its current-year shoots, which are tender, susceptible to pathogen infection, and contain many root-inhibiting substances, resulting in extremely low survival rates for conventional cuttings. Stable cultivation of this species has always been a challenge in the field. Existing research shows that even with plant hormone treatment, the highest rooting rate for cuttings is only 18.5% (Guo Xingfei. *Acer rubrum*, a rare ornamental plant). Acer griseum Research on the breeding technology of ) [D]. Henan University of Science and Technology, 2018.), and the related technologies do not clearly define key environmental parameters such as temperature and humidity, resulting in poor stability in actual operation and difficulty in commercialization.

[0004] Therefore, there is an urgent need to establish a set of efficient, stable, and large-scale application techniques for the propagation of blood maple by softwood cuttings. Summary of the Invention

[0005] This invention aims to overcome the problems of low rooting rate and unstable survival rate in existing Acer rubrum cutting propagation techniques, and provides a method to improve the rooting rate of Acer rubrum cuttings. This method significantly improves the rooting rate and survival rate of current-year tender shoot cuttings of Acer rubrum, providing reliable technical support for the efficient cultivation of its clonal seedlings.

[0006] To achieve this technical objective, the technical solution adopted by this invention is as follows: In a first aspect, the present invention provides a method for improving the rooting rate of *Acer rubrum* cuttings, comprising the following steps: Step 1: Obtain blood maple cuttings and disinfect them; Step 2: Treat the base of the cuttings with a rooting solution; the rooting solution contains ABT1 rooting powder at a final concentration of 100-200 mg / L, IBA at a final concentration of 150-200 mg / L, and ascorbic acid at a final concentration of 25-100 mg / L. Step 3: Plant the cuttings after they have been treated with rooting solution and manage them after planting.

[0007] Preferably, the blood-bark maple cuttings are 7-15 cm semi-lignified branches collected during the growing season.

[0008] Preferably, in step 1, the disinfection treatment involves soaking the *Acer rubrum* cuttings in a carbendazim solution diluted 800-1000 times for 20-30 minutes.

[0009] Optionally, in step 1, the disinfection method can also be selected as follows: using a strong disinfectant for quick application, such as treating the base of the cutting with a 0.1-0.3% potassium permanganate solution for 2-5 minutes, or immediately applying a paste or concentrated suspension of thiophanate-methyl to the cut part at the base of the cutting to form a protective film.

[0010] Preferably, in step 2, the base of the cutting is immersed in rooting solution for 1-2 hours.

[0011] More preferably, the rooting solution contains a mixed solution of ABT1 rooting powder with a final concentration of 150-200 mg / L, IBA with a final concentration of 150-180 mg / L, and ascorbic acid with a final concentration of 80-100 mg / L.

[0012] Preferably, in step 3, the cuttings are inserted into a mixed substrate containing peat moss, perlite, and vermiculite.

[0013] More preferably, the volume ratio of peat, perlite, and vermiculite in the mixed matrix is ​​6~8:2~4:4~6.

[0014] Preferably, in step 3, the control conditions for post-insertion management include: a temperature of 20℃~32℃ and a light intensity of (3~10)×10⁻⁶. 4 The lux and light duration are 4-10 hours. From 7:30 to 19:30 every day, the air humidity is maintained at 90±5%RH through an intermittent spray device. After 19:30, the intermittent spray device is turned off. The soil moisture is checked every 2-3 days, and watering is carried out as needed to maintain the soil moisture at 60%-70%RH. Under the above conditions, cultivation takes 30-60 days.

[0015] More preferably, in step 3, the control conditions for post-insertion management include: a temperature of 25℃~30℃ and a light intensity of (5~8)×10⁻⁶. 4 The lux and light duration are 5-7 hours. From 7:30 to 19:30 every day, the air humidity is maintained at 90±2%RH through an intermittent spray device. After 19:30, the intermittent spray device is turned off. The soil moisture is checked every 2 days and watered as needed to maintain the soil moisture at 60%~65%RH. Under the above conditions, cultivation takes 50-60 days.

[0016] Compared with the prior art, the present invention has the following technical effects: Firstly, by using this invention, the rooting rate of tender cuttings of Acer rubrum can be steadily increased from the current highest rate of 18.5% to 29.6%, a significant increase of 11.1%, providing an essential basis for the survival of Acer rubrum. This is a major breakthrough in the field and provides a feasible path to solve the problem of asexual reproduction of this tree species.

[0017] Secondly, this invention, through comprehensive disinfection and root-promoting treatment measures, combined with scientific substrate ratios and precise control of environmental parameters, forms a stable rooting microenvironment, significantly promoting the formation of adventitious roots and providing comprehensive protection for the survival of blood maple cuttings. Attached Figure Description

[0018] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this invention, illustrate exemplary embodiments of the invention and are used to explain the invention, but do not constitute an undue limitation of the invention. In the drawings: Figure 1 This image shows the rooting of *Acer rubrum* cuttings on day 38, obtained using a rooting solution containing 200 mg / L ABT1 rooting powder, 150 mg / L IBA, and 100 mg / L ascorbic acid, as described in Example 1 of this invention.

[0019] Figure 2 This is a rooting image of a single *Acer rubrum* cutting on the 40th day after cutting, as shown in Example 2 of this invention.

[0020] Figure 3 This is a photograph of some of the cutting seedlings obtained in Embodiment 2 of the present invention. Detailed Implementation

[0021] A specific embodiment of the present invention provides a method for improving the rooting rate of *Acer rubrum* cuttings, comprising the following steps: Step 1: During the growing season of Acer rubrum, collect 7-15 cm semi-lignified branch cuttings from the current year and disinfect the cuttings. The disinfection treatment is to soak the Acer rubrum cuttings in a carbendazim solution diluted 800-1000 times for 20-30 minutes.

[0022] Step 2: Immerse the base of the cuttings in rooting solution for 1-2 hours; the rooting solution contains ABT1 rooting powder at a final concentration of 100-200 mg / L, IBA at 150-200 mg / L, and ascorbic acid at 25-100 mg / L. Experiments have shown that adding a certain amount of ascorbic acid to the rooting solution can further improve the rooting rate of *Acer rubrum*.

[0023] Step 3: Plant the rooting solution-treated cuttings and manage them post-planting. The substrate used for cuttings must be loose, well-aerated, moisture-retentive, and sterile. The mixed substrate used in this invention contains peat moss, perlite, and vermiculite, with a volume ratio of 6-8:2-4:4-6. The specific operation for cuttings is as follows: First, use a thin stick to make a hole in the substrate, then place the cutting in, avoiding direct insertion that could damage the cut; the depth is generally 1 / 3 to 1 / 2 of the cutting's length. Compact the surrounding substrate to ensure close contact, and water thoroughly to ensure the substrate and cutting are in close contact.

[0024] Post-insertion management is the crucial stage that determines whether the *Acer rubrum* cuttings can successfully root and is a critical period for their survival rate. The core aspects are balancing moisture, aeration, and light. The control conditions for post-insertion management of *Acer rubrum* explored in this invention include: a temperature of 20℃~32℃ and a light intensity of (3~10)×10⁻⁶. 4 The lux and light duration are 4-10 hours. From 7:30 to 19:30 every day, the air humidity is maintained at 90±5%RH through an intermittent spray device. After 19:30, the intermittent spray device is turned off. The soil moisture is checked every 2-3 days, and watering is carried out as needed to maintain the soil moisture at 60%-70%RH. Under the above conditions, cultivation takes 30-60 days.

[0025] In this invention, the dilution factor refers to, for example, the dilution of 1 gram of carbendazim to 1 L of water is a 1000-fold dilution of carbendazim solution.

[0026] Furthermore, in the description of this invention, it should be noted that unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1

[0029] This embodiment provides an exploratory experiment on hormone induction for the current year's softwood cuttings of Acer rubrum, as detailed below: Step 1, Cutting preparation: Select semi-lignified current-year shoots as cuttings during the growing season, retaining 1-2 complete leaves at the top (if the leaves are large, they can be cut in half), and remove the rest of the leaves.

[0030] Step 2, disinfection treatment: Immerse the base of the cuttings in a 1000-fold diluted carbendazim solution for 30 minutes to achieve effective surface sterilization.

[0031] Step 3, Hormone Induction: The base of the disinfected cuttings was immersed in different concentrations of IAA solution (50, 100, 200 mg / L), different concentrations of IBA solution (100, 150, 200 mg / L), and different concentrations of ABT1 rooting powder (100, 200, 300 mg / L) for 1-2 hours. The rooting rate was calculated after all steps were completed. A control group (CK) was set up, with the water-immersed base of the cuttings. Each group had three replicates, with 20 cuttings per replicate. The average rooting rate was calculated. The results are shown in Table 1.

[0032] Step 4, Cuttings: Insert the hormone-treated cuttings into the pre-sterilized seedling substrate; the seedling substrate is prepared by mixing peat moss, perlite and vermiculite in a volume ratio of 7:3:5.

[0033] Step 5, Post-cutting Management: After cutting, place the seedbed in an environment of 25℃~30℃ for cultivation, and control the light intensity at 6×10. 4 The lux and light duration are 6-7 hours. From 7:30 AM to 7:30 PM daily, maintain air humidity at 90±2% RH using an intermittent misting system (any commercially available system will work). After 7:30 PM, turn off the intermittent misting system. Check soil moisture every two days and water as needed to maintain soil moisture at 60%-65% RH. Calculate the rooting rate of *Acer rubrum* cuttings within 60 days of planting.

[0034] Table 1. Rooting results of a single hormone

[0035] The data in Table 1 show that when using a single hormone, the rooting rates of IAA (final concentration 100 mg / L), IBA (final concentration 150 mg / L), and ABT1 (final concentration 200 mg / L) were the best, reaching 8.3%, 13.3%, and 18.3%, respectively.

[0036] Furthermore, the hormones obtained from Table 1 at their optimal final concentrations were combined in pairs: 100 mg / L IAA + 150 mg / L IBA solution, 100 mg / L IAA + 200 mg / L ABT1 solution, and 200 mg / L ABT1 + 150 mg / L IBA solution (where each hormone concentration is the final concentration in the combined solution). The optimal hormone combination was then determined again through steps 2-5, with three replicates per group and 16 cuttings per replicate. The average rooting rate was calculated. The results are shown in Table 2.

[0037] Table 2. Rooting results of hormone combinations

[0038] The data in Table 2 show that the rooting solution of 200 mg / L ABT1 + 150 mg / L IBA yielded the best rooting rate, which could increase the rooting rate from below 20% with a single hormone to 22.9%, demonstrating a significant effect.

[0039] This embodiment further investigated the rooting rate of the rooting solution of 100 mg / L ABT1 + 200 mg / L IBA based on the aforementioned experiments. The results are shown in Table 2, indicating that the rooting solution of 100 mg / L ABT1 + 200 mg / L IBA can also achieve a rooting rate of over 20%.

[0040] The optimal hormone combination for rooting rate was further mixed with ascorbic acid solutions of different final concentrations (25-100 mg / L) and the base of the cuttings was soaked for 1-2 hours. The optimal hormone combination was then determined again through steps 2-5. Each group was set up with 3 replicates, and each replicate had 45 cuttings. The average rooting rate was calculated. The results are shown in Table 3.

[0041] Table 3. Effects of different concentrations of ascorbic acid on rooting rate.

[0042] Table 3 shows that the rooting rate was above 23.5% when the final concentration of ascorbic acid was 25-100 mg / L, which is significantly higher than the previously reported rooting rate of 18.5%. The rooting rate was the best in the rooting system containing 100 mg / L of ascorbic acid. Therefore, the final concentration of ascorbic acid can be controlled in the range of 25-100 mg / L, preferably in the range of 80-100 mg / L. Figure 1 Images of a *Acer rubrum* cutting taken 38 days after rooting in a rooting solution containing 200 mg / L ABT1 rooting powder, 150 mg / L IBA, and 100 mg / L ascorbic acid are provided.

[0043] In summary, the rooting solution of the present invention preferably has the following final concentration composition: 150-200 mg / L of ABT1 rooting powder, 150-180 mg / L of IBA and 80-100 mg / L of ascorbic acid, with water as the solvent.

[0044] Comparative Example 1 Based on the use of a rooting solution consisting of 200 mg / L ABT1 rooting powder, 150 mg / L IBA, and 100 mg / L ascorbic acid in water, this comparative study further investigated the effect of post-planting management control conditions on the rooting rate, as detailed below: This comparative example sets up 3 replicate experimental groups. The difference in the operation steps between the experimental groups and Example 1 is step 5: using a relative illuminance of 12.36% of full illumination (0.74×10⁻⁶). 4 (lux), otherwise the same as in Example 1. Each replicate group consisted of 45 cuttings, and the average rooting rate was calculated. The results are shown in Table 4.

[0045] Table 4 Rooting rate under different post-insertion management

[0046] Table 4 shows that the rooting rate decreased by nearly 9% after partial shading of the cuttings. This data indicates that *Acer rubrum* is suitable for cultivation in an environment with slightly higher light intensity. In this invention, the light intensity was set at (3~10) × 10⁻⁶. 4 lux, preferably (5~8)×10 4 lux.

[0047] Example 2

[0048] This embodiment provides a method for improving the rooting rate of *Acer rubrum* cuttings. The experiment was conducted at an experimental base on Jiangjun Avenue, Bazhou District, Bazhong City, Sichuan Province, and specifically includes the following steps: a) Disinfection of cuttings: Soak the cuttings in a 1000-fold carbendazim solution for 30 minutes; the cuttings are semi-lignified current-year shoots collected during the growing season, totaling 300 cuttings.

[0049] b) Hormone treatment: Soak the base of the cuttings in a mixed solution of 200 mg / L ABT1 rooting powder, 150 mg / L IBA and 100 mg / L ascorbic acid for 1.5 hours.

[0050] c) Cuttings: Insert the cuttings into a mixed substrate prepared by peat moss, perlite and vermiculite in a certain volume ratio; the volume ratio of peat moss, perlite and vermiculite in the mixed substrate is 7:3:5.

[0051] d) Post-insertion management: At a temperature of 25℃~30℃, the light intensity is (5~8)×10 4 The lux and light duration are 5-7 hours. From 7:30 to 19:30 every day, the air humidity is maintained at 90±2%RH through an intermittent spray device. After 19:30, the intermittent spray device is turned off. The soil moisture is checked every 2 days, and watering is carried out as needed to maintain the soil moisture at 60%~65%RH. Figure 2 Images of a single blood maple cutting rooted on day 40 are provided.

[0052] Statistical data: According to the above treatment plan, after 60 days of post-planting management and cultivation, the rooting rate of softwood cuttings of *Acer rubrum* reached 29.6%, with an average of 2.40 roots per plant. After 90 days, the survival rate was 85%. Some of the cultivated seedlings are shown below. Figure 3 As shown.

[0053] In summary, the cutting rooting method of the present invention can steadily increase the rooting rate of Acer rubrum cuttings from the current highest rate of 18.5% to 29.6%, a significant increase of 11.1%, providing a feasible path to solve the problem of asexual reproduction of this tree species.

[0054] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents. The embodiments described above merely illustrate several implementations of the invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the invention, and these all fall within the protection scope of the invention. Therefore, the protection scope of this invention should be determined by the appended claims.

Claims

1. A method for improving the rooting rate of Acer griseum cuttings, characterized in that, It comprises the following steps: Step 1, obtaining blood Acer truncatum cutting, sterilizing the blood Acer truncatum cutting; Step 2, treating the base of the cutting with rooting liquid; the rooting liquid contains ABT1 rooting powder with a final concentration of 100-200 mg / L, IBA with a final concentration of 150-200 mg / L and ascorbic acid with a final concentration of 25-100 mg / L; Step 3, cutting and post-cutting management of the cutting treated with the rooting liquid.

2. The method of claim 1, wherein, The blood Acer truncatum cutting is a 7-15 cm semi-lignified branch collected in the growing season.

3. The method of claim 1, wherein, In step 1, the sterilization treatment is soaking the blood Acer truncatum cutting in a diluted 800-1000 times carbendazim solution for 20-30 min.

4. The method of claim 1, wherein, In step 2, the base of the cutting is soaked in the rooting liquid for 1-2 h.

5. The method of claim 4, wherein, The rooting liquid contains a mixed solution of ABT1 rooting powder with a final concentration of 150-200 mg / L, IBA with a final concentration of 150-180 mg / L and ascorbic acid with a final concentration of 80-100 mg / L.

6. The method of claim 1, wherein, In step 3, the cutting is cut in a mixed substrate containing peat soil, perlite and vermiculite.

7. The method of claim 6, wherein, The volume ratio of peat soil, perlite and vermiculite in the mixed substrate is 6-8:2-4:4-6.

8. The method of claim 1, wherein, The control condition of the plug post-management in step 3 comprises: temperature is 20-32℃, light intensity is (3-10)×10 4 lux, light time is 4-10h, air humidity is kept at 90±5%RH by intermittent spraying device from 7:30 to 19:30 every day, the intermittent spraying device is closed after 19:30, soil humidity is detected every 2-3 days, and water is timely poured to keep soil humidity at 60%-70%RH; under the foregoing conditions, cultivation is carried out for 30-60 days.

9. The method of claim 8, wherein, The control condition of the plug post-management in step 3 comprises: temperature is 25-30℃, light intensity is (5-8)×10 4 lux, light time is 5-7h, air humidity is kept at 90±2%RH by intermittent spraying device from 7:30 to 19:30 every day, the intermittent spraying device is closed after 19:30, soil humidity is detected every 2 days, and water is timely poured to keep soil humidity at 60-65%RH; under the foregoing conditions, cultivation is carried out for 50-60 days.

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