Ginseng adventitious root culture device

Abstract

The invention relates to a ginseng adventitious root culture device, and belongs to the technical field of biological culture, the ginseng adventitious root culture device comprises an inoculation tank, a lifting rod, a culture tank, a connecting cylinder and a culture valve, the inoculation tank is provided with a seed falling port and a lifting port; the lifting rod penetrates through the lifting opening in a sliding manner and is fixed with an inoculation plug; the culture tank is provided with a seed inlet and an operation port; two ends of the connecting cylinder are respectively butted with the seed falling opening and the seed inlet; the culture valve comprises a rotating rod, a swinging arm and a culture plug; the rotating rod is rotationally connected in the operation opening, and a driving gear meshed with the driven gear is fixed at the bottom end of the rotating rod; one end of the swinging arm is fixed on the rotating shaft, and a culture plug is fixed at the other end. The lifting rod is used for controlling the inoculation plug to open and close the seed falling opening, and then the rotating rod is used for driving the culture plug to open and close the seed inlet, so that closed transfer of ginseng adventitious roots between the inoculation tank and the culture tank can be realized, infectious microbe pollution is effectively prevented, and the transfer safety is improved.

Description

Technical Field

[0001] This invention relates to the field of bioculture technology, and in particular to a ginseng adventitious root culture device. Background Technology

[0002] Ginseng, as an important medicinal plant, contains ginsenosides and other secondary metabolites in its roots that have significant pharmacological activity. To address the shortage of wild and cultivated ginseng resources, the use of plant tissue culture technology to cultivate ginseng adventitious roots under in vitro conditions has become a sustainable alternative for obtaining ginseng's active ingredients.

[0003] To achieve the industrial application of this technology, it is usually necessary to scale up the laboratory-scale culture system to a bioreactor for large-scale fermentation. Airlift bioreactors and mechanically stirred bioreactors are commonly used in the industrial cultivation of ginseng adventitious roots. Traditional airlift bioreactors are widely used, but they have inherent defects. During the cultivation process, dead air zones are easily generated, causing ginseng adventitious roots to aggregate within the reactor. Aggregation severely hinders the transfer of materials and energy and the efficiency of dissolved oxygen in that area, leading to physiological and morphological abnormalities of the adventitious roots and the production of a large amount of detached material. Although mechanically stirred bioreactors can effectively improve material mixing and prevent root aggregation, the fluid shear force they generate can cause mechanical damage to ginseng adventitious roots, resulting in low biomass and insufficient saponin synthesis, thus increasing production costs. In addition, during the inoculation and transfer process, both airlift and mechanically stirred bioreactors require the transfer of inoculum from the inoculation stage to the cultivation stage. This is not only complex, cumbersome, and inefficient, but also further increases the risk of contamination.

[0004] Therefore, how to design a bioreactor that is not prone to agglomeration or breakage, has high transfer efficiency, and strong operational safety is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] This invention provides a ginseng adventitious root culture device, which solves the technical problems of existing ginseng adventitious roots being prone to agglomeration, easy to be cut and damaged, difficult to transport, and easy to be contaminated.

[0006] The technical solution of this invention to solve the above-mentioned technical problems is as follows: a ginseng adventitious root culture device, comprising: an inoculation tank, an inoculation valve, a culture tank, a connecting cylinder, and a culture valve; the inoculation tank has a seed-dropping port at its bottom end and a lifting port and an inoculation port at its top end; the inoculation valve includes a lifting rod and an inoculation plug; the bottom end of the lifting rod slides through the lifting port and extends into the inoculation tank; the inoculation plug is fixed to the bottom end of the lifting rod extending into the inoculation tank and can be sealed and inserted into the seed-dropping port; the culture tank is located below the inoculation tank and has a seed inlet and an operation port at its top end; the top end of the connecting cylinder corresponds to the outer periphery of the seed-dropping port and abuts against the bottom end of the inoculation tank, and its bottom end corresponds to the seed inlet... The outer periphery of the inoculation port abuts against the top of the culture tank; the culture valve includes a rotating rod, a driven gear, a swing arm, and a culture plug. The rotating rod is rotatably connected inside the operating port, and a driving gear is fixed at its end extending into the bottom of the culture tank; the driven gear is rotatably connected inside the culture tank via a rotating shaft with its axis perpendicular to the height of the culture tank as the axis and meshes with the driving gear; the swing arm is located inside the culture tank, and one end of its arm is fixed to the rotating shaft; the culture plug is located inside the culture tank and fixed to the other end of the swing arm, so that it is sealed and inserted into the inoculation port as the swing arm swings, thereby sealingly transferring the ginseng adventitious roots in the inoculation tank to the culture tank.

[0007] The beneficial effects of this invention are: it improves the structure of traditional ginseng adventitious root bioreactors. First, a lifting rod is used to control the opening and closing of the inoculation plug, and then a rotating rod is used to drive the culture plug to open and close the inlet via a driving gear, a driven gear, and a swing arm. Since the two ends of the connecting cylinder are arranged opposite to the inoculation plug and the inlet, the ginseng adventitious roots in the inoculation tank can be sealed and transported to the culture tank. This improves the transport efficiency while preventing external bacteria from entering the culture tank, thus enhancing the safety of ginseng adventitious root transport.

[0008] Based on the above technical solution, the present invention can be further improved as follows.

[0009] Furthermore, it also includes an inoculation air tube, an inoculation air pump, an inoculation filter, and an inoculation bubble generator. The inoculation air tube has a ring structure and is fixed outside the inoculation container. The inoculation air tube is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the inoculation air pump, the inoculation filter, and the inoculation bubble generator are connected in series, and the air outlet of the inoculation bubble generator is connected to the air inlet of the inoculation air tube. Multiple spaced-apart inoculation air ports are provided on the lower outer periphery of the inoculation container. The multiple air outlets of the inoculation air tube are respectively connected to the multiple inoculation air ports of the inoculation container through multiple connecting pipes.

[0010] The further beneficial effects of the above-mentioned method are: by using the inoculation air pipe, inoculation air pump, inoculation filter and inoculation bubble generator in combination, the oxygen flow pattern in the inoculation tank is changed, avoiding the damage of ginseng adventitious roots by airflow shear force; at the same time, the inoculation air ports distributed at intervals on the lower outer periphery of the inoculation tank provide air evenly into the inoculation tank, which can prevent ginseng adventitious roots from agglomerating and increase the biomass and saponin yield of ginseng adventitious roots.

[0011] Furthermore, it also includes an inoculation pH sensor and a culture pH sensor. The top of the inoculation vessel is provided with a detection hole; the inoculation pH sensor is fixed at the detection hole and its sensing probe extends into the inoculation vessel; the top of the culture vessel is provided with a monitoring hole; the culture pH sensor is fixed at the monitoring hole and its sensing probe extends into the culture vessel.

[0012] Furthermore, it also includes an inoculation vent valve and a culture vent valve. The top of the inoculation tank is provided with a vent port; the inoculation vent valve is fixed at the vent port; the top of the culture tank is provided with an exhaust port; and the culture vent valve is fixed at the exhaust port.

[0013] The further beneficial effect of adopting the above is that by fixing the inoculation vent valve and the culture vent valve at the vent of the inoculation tank and the exhaust port of the culture tank respectively, excessive pressure inside the inoculation tank and the culture tank can be avoided.

[0014] Furthermore, it also includes an inoculation heating rod, an inoculation temperature controller, a culture heating rod, and a culture temperature controller, wherein the inoculation heating rod is placed inside the inoculation tank; the inoculation temperature controller is fixed outside the inoculation tank and electrically connected to the inoculation heating rod; the culture heating rod is placed inside the culture tank; and the culture temperature controller is fixed outside the culture tank and electrically connected to the culture heating rod.

[0015] The further beneficial effects of the above are: by using the inoculation temperature controller in conjunction with the inoculation heating rod, the internal temperature of the inoculation tank can be precisely controlled; at the same time, by using the culture temperature controller in conjunction with the culture heating rod, the internal temperature of the culture tank can be precisely controlled.

[0016] Furthermore, it also includes a culture gas tube, a culture gas pump, a culture filter, and a culture bubble generator. The culture gas tube has a ring structure and is arranged below the culture tank. The culture gas tube is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the culture gas pump, the culture filter, and the culture bubble generator are connected in series, and the air outlet of the culture bubble generator is connected to the air inlet of the culture gas tube. Multiple spaced culture gas ports are provided on the lower outer periphery of the culture tank. The multiple air outlets of the culture gas tube are connected to the multiple culture gas ports of the culture tank through connecting pipes.

[0017] The further beneficial effects of the above are: by using the culture air tube, culture air pump, culture filter and culture bubble generator in combination, the oxygen flow pattern in the culture tank is changed, avoiding the damage of ginseng adventitious roots by airflow shear force; at the same time, the culture air ports distributed at intervals on the lower outer periphery of the culture tank provide air evenly into the culture tank, which can prevent ginseng adventitious roots from agglomerating and increase the biomass and saponin yield of ginseng adventitious roots.

[0018] Furthermore, it also includes a peristaltic pump and a chromatography apparatus. The bottom of the culture tank is provided with a drain port and the upper side is provided with an inlet port. The inlet port of the peristaltic pump is connected to the drain port of the culture tank. The inlet port of the chromatography apparatus is connected to the outlet port of the peristaltic pump and its outlet port is connected to the inlet port of the culture tank, so as to separate the saponins contained in the culture medium in the culture tank.

[0019] The further beneficial effect of the above method is that the culture medium in the culture tank is first extracted by a peristaltic pump, and then the saponins in the culture medium are separated by a chromatography apparatus, which can avoid the culture medium in the culture tank being too high and affecting the growth of ginseng.

[0020] Furthermore, a steam inlet is provided on the liquid pipe between the drain port of the culture tank and the inlet of the peristaltic pump.

[0021] Furthermore, it also includes a cooling shell, which is wrapped and fixed to the outer periphery of the culture tank and defines a cooling interlayer between the culture tank and the cooling shell. The upper side of the cooling shell is provided with a refrigerant inlet and the lower side is provided with a refrigerant outlet. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the internal structure of the culture plug sealing the seed inlet in a ginseng adventitious root culture device of the present invention;

[0023] Figure 2 This is a schematic diagram of the internal structure of the culture plug opening the seed inlet in a ginseng adventitious root culture device of the present invention; Figure 3 This is a schematic diagram of the internal structure of the culture tank in a ginseng adventitious root culture device of the present invention; Figure 4 This is a schematic diagram of the internal structure of the inoculation tank in a ginseng adventitious root culture device of the present invention.

[0024] The attached diagram lists the components represented by each number as follows: 1. Inoculation tank; 11. Seed inlet; 12. Inoculation air inlet; 2. Inoculation valve; 21. Lifting rod; 22. Inoculation plug; 3. Culture tank; 31. Seed inlet; 32. Culture air inlet; 4. Connecting cylinder; 5. Culture valve; 51. Rotating rod; 52. Driven gear; 53. Swing arm; 54. Culture plug; 55. Driving gear; 6. Inoculation air tube; 7. Inoculation pH sensor; 8. Culture pH sensor; 9. Inoculation vent valve; 10. Culture vent valve; 13. Inoculation temperature controller; 14. Culture temperature controller; 15. Inoculation outer cover; 16. Culture outer cover; 17. Culture air tube; 18. Peristaltic pump; 19. Chromatography unit; 20. Steam inlet; 23. Cooling shell. Detailed Implementation

[0025] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0026] like Figure 1 and Figure 2 As shown, a ginseng adventitious root culture device includes: an inoculation tank 1, an inoculation valve 2, a culture tank 3, a connecting cylinder 4, and a culture valve 5. The inoculation tank 1 has a seed inlet 11 at its bottom end and a lifting port at its top end. The inoculation valve 2 includes a lifting rod 21 and an inoculation plug 22. The bottom end of the lifting rod 21 slides through the lifting port and extends into the inoculation tank 1. The inoculation plug 22 is fixed to the bottom end of the lifting rod 21 that extends into the inoculation tank 1 and can seal the insertion into the seed inlet 11. The culture tank 3 is located below the inoculation tank 1 and has a seed inlet 31 and an operation port at its top end. The top end of the connecting cylinder 4 is in contact with the bottom end of the inoculation tank 1 at the outer periphery of the seed inlet 11, and the bottom end is in contact with the outer periphery of the seed inlet 31 at the outer periphery of the culture valve 5. The top of the tank 3 is in contact with the inoculation vessel; the culture valve 5 includes a rotating rod 51, a driven gear 52, a swing arm 53, and a culture plug 54. The rotating rod 51 is rotatably connected to the operating port and the bottom end of the rotating rod extending into the culture tank 3 is fixed with a driving gear 55; the driven gear 52 is rotatably connected to the culture tank 3 via a rotating shaft with the axis perpendicular to the height of the culture tank 3 as the axis and is meshed with the driving gear 55; the swing arm 53 is located inside the culture tank 3 and one end is fixed to the rotating shaft; the culture plug 54 is located inside the culture tank 3 and is fixed to the other end of the swing arm 53 so that it is sealed and inserted into the inoculation port 31 as the swing arm 53 swings, so that the inoculation seal in the inoculation tank 1 is transferred to the culture tank 3.

[0027] like Figure 4As shown, in some specific embodiments, it may also include an inoculation air tube 6, an inoculation air pump, an inoculation filter, and an inoculation bubble generator. The inoculation air tube 6 has a ring structure and is sleeved outside the inoculation tank 1. The inoculation air tube 6 is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the inoculation air pump, the inoculation filter, and the inoculation bubble generator are connected in series, and the air outlet of the inoculation bubble generator is connected to the air inlet of the inoculation air tube 6. Multiple inoculation air ports 12 are provided at intervals on the lower outer periphery of the inoculation tank 1. The multiple air outlets of the inoculation air tube 6 are respectively connected to the multiple inoculation air ports 12 of the inoculation tank 1 through multiple connecting pipes.

[0028] like Figure 3 and Figure 4 As shown, in some specific embodiments, it may also include an inoculation pH sensor 7 and a culture pH sensor 8. The top of the inoculation tank 1 is provided with a detection hole; the inoculation pH sensor 7 is fixed at the detection hole and its sensing probe extends into the inoculation tank 1; the top of the culture tank 3 is provided with a monitoring hole; the culture pH sensor 8 is fixed at the monitoring hole and its sensing probe extends into the culture tank 3.

[0029] like Figure 3 and Figure 4 As shown, in some specific embodiments, it also includes an inoculation vent valve 9 and a culture vent valve 10. The top of the inoculation tank 1 is provided with a vent; the inoculation vent valve 9 is fixed at the vent; the top of the culture tank 3 is provided with an exhaust port; and the culture vent valve 10 is fixed at the exhaust port.

[0030] like Figure 3 and Figure 4 As shown, in some specific embodiments, it may also include an inoculation heating rod, an inoculation temperature controller 13, a culture heating rod, and a culture temperature controller 14. The inoculation heating rod is placed inside the inoculation tank 1; the inoculation temperature controller 13 is fixed outside the inoculation tank 1 and electrically connected to the inoculation heating rod; the culture heating rod is placed inside the culture tank 3; and the culture temperature controller 14 is fixed outside the culture tank 3 and electrically connected to the culture heating rod.

[0031] like Figure 3 and Figure 4 As shown, in some specific embodiments, it may also include an inoculation outer cover 15 and a culture outer cover 16. The inoculation outer cover 15 is detachably covered at the seed inlet 11 of the inoculation tank 1; the culture outer cover 16 is detachably covered at the seed inlet 31 of the culture tank 3.

[0032] like Figure 4As shown, in some specific embodiments, it may also include a culture gas tube 17, a culture gas pump, a culture filter, and a culture bubble generator. The culture gas tube 17 has a ring structure and is arranged below the culture tank 3. The culture gas tube 17 is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the culture gas pump, culture filter, and culture bubble generator are connected in series, and the air outlet of the culture bubble generator is connected to the air inlet of the culture gas tube 17. Multiple culture gas ports 32 are provided at intervals on the lower outer periphery of the culture tank 3. The multiple air outlets of the culture gas tube 17 are connected to the multiple culture gas ports 32 of the culture tank 3 through connecting pipes.

[0033] like Figure 1 and Figure 2 As shown, in some specific embodiments, a peristaltic pump 18 and a chromatography tank 19 may also be included. The bottom of the culture tank 3 is provided with a drain port and the upper side is provided with an inlet port. The inlet port of the peristaltic pump 18 is connected to the drain port of the culture tank 3. The inlet port of the chromatography tank 19 is connected to the outlet port of the peristaltic pump 18 and its outlet port is connected to the inlet port of the culture tank 3, so as to separate the saponins contained in the culture medium in the culture tank 3.

[0034] like Figure 1 and Figure 2 As shown, in some specific embodiments, a steam inlet 20 is provided on the liquid pipe between the drain port of the culture tank 3 and the inlet of the peristaltic pump 18.

[0035] In some specific embodiments, a cooling shell 23 is also included. The cooling shell 23 is wrapped and fixed to the outer periphery of the culture tank 3 and defines a cooling interlayer between the culture tank 3 and the cooling shell 23. The upper side of the cooling shell 23 is provided with a refrigerant inlet and the lower side is provided with a refrigerant outlet.

[0036] Description of the operation process: I. Preparation Phase 1. System Assembly: Assemble the inoculation tank 1 and culture tank 3 by connecting them together with the connecting cylinder 4, ensuring that the seed inlet 11 is aligned with the seed outlet 31. Install the inoculation valve 2, culture valve 5, and all auxiliary components such as sensors and pipelines.

[0037] 2. Initial state settings: Inoculation tank 1: The lifting rod 21 is in the lifting state, and the inoculation plug 22 seals the seed drop port 11. The inoculation outer cover 15 is closed on the outside of the seed drop port 11. Sterilized culture medium is added into the inoculation tank 1 through the inoculation port (not shown in the figure), and ginseng adventitious roots are inoculated.

[0038] Culture tank 3: Driven by rotating rod 51, swing arm 53 drives culture plug 54 to seal and insert into inlet 31. Culture outer cover 16 covers the outside of inlet 31, and sterilized fresh culture medium is added into culture tank 3.

[0039] 3. Environmental Parameter Preset: The appropriate temperature inside the tank is set and maintained via inoculation temperature controller 13 and culture temperature controller 14, respectively. The inoculation and culture air pumps are turned on, providing a sterile and uniform dispersed airflow into the tank through the filter and bubble generator to prevent adventitious root aggregation. The inoculation vent valve 9 and culture vent valve 10 regulate the pressure inside the tank. The inoculation pH sensor 7 and culture pH sensor 8 monitor the pH value.

[0040] II. Inoculation and Culture Stage 1. Pre-culture in inoculation tank: In inoculation tank 1, ginseng adventitious roots undergo preliminary growth and propagation under controlled temperature, pH and aeration conditions.

[0041] 2. Sealed transport: Step 1: Confirm that the culture environment inside culture tank 3 is stable. Remove the inoculation cover 15 and the culture cover 16.

[0042] Step 2: Open the inlet: Operate the rotating rod 51 from the operating port on the top of the culture tank 3 to drive the drive gear 55 to rotate, which in turn drives the driven gear 52 that meshes with it to rotate, thereby causing the swing arm 53 to swing and remove the culture plug 54 from the inlet 31.

[0043] Step 3: Open the seed inlet: Pull the lever 21 on the top of the inoculation container 1 upwards to pull the inoculation plug 22 out of the seed inlet 11.

[0044] Step 4: Gravity seeding: Under the action of gravity, the ginseng adventitious roots in the inoculation tank 1, along with some culture solution, fall directly into the culture tank 3 below through the connected seeding port 11, connecting tube 4 and seed inlet 31 in a closed system.

[0045] Step 5: Close the valve: First push the lifting rod 21 downward to make the inoculation plug 22 reseal the seed inlet 11, then reverse the operation of the rotating rod 51 to make the culture plug 54 reseal the seed inlet 31.

[0046] III. Master Culture and Product Extraction: Ginseng adventitious roots were cultured on a large scale in culture tank 3, and various environmental parameters (temperature, aeration, pH) were maintained and optimized by the corresponding system. Online extraction: Start the peristaltic pump 18 to pump the culture medium containing saponins from the bottom of the culture tank 3 and deliver it to the chromatography unit 19 for the separation and enrichment of saponins. The processed culture medium (or fresh replenishment medium) can be returned to the inlet at the top of the culture tank 3 to realize the circulation and renewal of the culture medium. The steam inlet 20 can be used to sterilize the connecting pipelines. Temperature control: If the heat generated during the cultivation process requires cooling, a cooling medium (such as cooling water) can be introduced through the cooling medium inlet of the cooling shell 23, circulated in the jacket and discharged from the cooling medium outlet to provide cooling for the cultivation tank 3.

[0047] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A ginseng adventitious root culture device, characterized in that, include: The inoculation tank (1) and the inoculation valve (2) are provided. The bottom end of the inoculation tank (1) is provided with a seed drop port (11) and the top end is provided with a lifting port. The inoculation valve (2) includes a lifting rod (21) and an inoculation plug (22). The bottom end of the lifting rod (21) slides through the lifting port and extends into the inoculation tank (1). The inoculation plug (22) is fixed to the bottom end of the lifting rod (21) extending into the inoculation tank (1) and can be sealed and inserted into the seed drop port (11). The culture tank (3), connecting cylinder (4), and culture valve (5) are provided. The culture tank (3) is located below the inoculation tank (1) and has an inlet (31) and an operating port at its top. The top of the connecting cylinder (4) is in contact with the bottom of the inoculation tank (1) at the outer periphery of the seed inlet (11), and the bottom of the connecting cylinder is in contact with the top of the culture tank (3) at the outer periphery of the inlet (31). The culture valve (5) includes a rotating rod (51), a driven gear (52), a swing arm (53), and a culture plug (54). The rotating rod (51) is rotatably connected to the operating port and extends into the culture tank (3). A drive gear (55) is fixed at the bottom end; the driven gear (52) is rotatably connected to the culture tank (3) via a rotating shaft with the axis perpendicular to the height of the culture tank (3) as the axis and meshes with the drive gear (55); the swing arm (53) is located inside the culture tank (3) and one end is fixed on the rotating shaft; the culture plug (54) is located inside the culture tank (3) and fixed at the other end of the swing arm (53) so as to be sealed and inserted into the inlet (31) as the swing arm (53) swings, so that the inoculation seal in the inoculation tank (1) is transferred to the culture tank (3).

2. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes an inoculation air tube (6), an inoculation air pump, an inoculation filter, and an inoculation bubble generator. The inoculation air tube (6) has a ring structure and is fitted outside the inoculation tank (1). The inoculation air tube (6) is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the inoculation air pump, the inoculation filter, and the inoculation bubble generator are connected in series, and the air outlet of the inoculation bubble generator is connected to the air inlet of the inoculation air tube (6). Multiple inoculation air ports (12) are provided at intervals on the lower outer periphery of the inoculation tank (1). The multiple air outlets of the inoculation air tube (6) are respectively connected to the multiple inoculation air ports (12) of the inoculation tank (1) through multiple connecting pipes.

3. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes an inoculation pH sensor (7) and a culture pH sensor (8). The top of the inoculation tank (1) is provided with a detection hole; the inoculation pH sensor (7) is fixed at the detection hole and its sensing probe extends into the inoculation tank (1); the top of the culture tank (3) is provided with a monitoring hole; the culture pH sensor (8) is fixed at the monitoring hole and its sensing probe extends into the culture tank (3).

4. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes an inoculation vent valve (9) and a culture vent valve (10). The top of the inoculation tank (1) is provided with a vent; the inoculation vent valve (9) is fixed at the vent; the top of the culture tank (3) is provided with an exhaust port; and the culture vent valve (10) is fixed at the exhaust port.

5. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes an inoculation heating rod, an inoculation temperature controller (13), a culture heating rod, and a culture temperature controller (14). The inoculation heating rod is placed inside the inoculation tank (1). The inoculation temperature controller (13) is fixed outside the inoculation tank (1) and electrically connected to the inoculation heating rod. The culture heating rod is placed inside the culture tank (3). The culture temperature controller (14) is fixed outside the culture tank (3) and electrically connected to the culture heating rod.

6. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes an inoculation cover (15) and a culture cover (16), wherein the inoculation cover (15) is detachably installed at the seed inlet (11) of the inoculation tank (1); and the culture cover (16) is detachably installed at the seed inlet (31) of the culture tank (3).

7. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes a culture gas tube (17), a culture gas pump, a culture filter, and a culture bubble generator. The culture gas tube (17) has a ring structure and is arranged below the culture tank (3). The culture gas tube (17) is provided with an air inlet and multiple air outlets. The air outlets and air inlets of the culture gas pump, the culture filter, and the culture bubble generator are connected in series, and the air outlet of the culture bubble generator is connected to the air inlet of the culture gas tube (17). Multiple culture gas ports (32) are provided at intervals on the lower outer periphery of the culture tank (3). The multiple air outlets of the culture gas tube (17) are connected to the multiple culture gas ports (32) of the culture tank (3) through connecting pipes.

8. The ginseng adventitious root culture device according to claim 1, characterized in that, It also includes a peristaltic pump (18) and a chromatography unit (19). The bottom of the culture tank (3) is provided with a drain port and the upper side is provided with an inlet port. The inlet port of the peristaltic pump (18) is connected to the drain port of the culture tank (3). The inlet port of the chromatography unit (19) is connected to the outlet port of the peristaltic pump (18) and its outlet port is connected to the inlet port of the culture tank (3) to separate the saponins contained in the culture medium in the culture tank (3).

9. A ginseng adventitious root culture device according to claim 8, characterized in that, A steam inlet (20) is provided on the liquid pipe between the drain port of the culture tank (3) and the inlet of the peristaltic pump (18).

10. A ginseng adventitious root culture device according to claim 1, characterized in that, It also includes a cooling shell (23), which is wrapped and fixed to the outer periphery of the culture tank (3) and defines a cooling interlayer between the culture tank (3) and the cooling shell (23). The upper side of the cooling shell (23) is provided with a refrigerant inlet and the lower side is provided with a refrigerant outlet.

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