Application of enzymolysis composite carbon source in prawn culture

Abstract

The invention relates to a divisional application with the application number of 2024110933028, and relates to the technical field of prawn aquaculture, in particular to application of an enzymolysis composite carbon source in prawn aquaculture. The application comprises a pond, and the pond is provided with a liquid oxygen supply assembly, an oxygenation pump assembly and a feed spreading device; the liquid oxygen supply assembly comprises a liquid oxygen input device arranged on one side of the pond and a liquid oxygen output device arranged on the liquid level of the pond, an organic carbon source is artificially added into the aquaculture water, the number of heterotrophic bacteria in the water is increased, nitrogen-containing compounds such as ammonia nitrogen in the water are converted into mycoprotein, and the liquid oxygen supply assembly is used for supplying liquid oxygen to the pond. According to the present invention, the biological flocculant capable of being directly ingested by the filter-feeding culture object is formed, such that the problem of detritus and feed retention in the culture water body can be solved, the bait reutilization can be achieved, the effects of water quality purification, water change amount reduction, feed saving, culture object survival rate improvement, yield increase and the like can be achieved, and the gastrointestinal tract load of the shrimp can be reduced through the automatic feeding; the nutrient absorption rate of the feed is improved, and the pollution to water quality is reduced.

Description

[0001] This application is a divisional application. The original application has the application number 2024110933028, the application date is August 9, 2024, and the invention title is "Application of an enzymatic hydrolysis composite carbon source in shrimp farming and its farming method". Technical Field

[0002] This invention relates to the field of shrimp aquaculture technology, specifically the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. Background Technology

[0003] The Pacific white shrimp (Litopenaeus vannamei) possesses advantages such as strong vitality, wide adaptability to salinity and temperature, strong disease resistance, high survival rate, rapid growth, high meat yield, long survival time out of water, and suitability for high-density farming. It is the most widely farmed shrimp species in my country, achieving significant economic and social benefits. With the large-scale development of shrimp farming, commercial aquaculture is mainly based on high-density, intensive farming, greatly increasing the yield of Pacific white shrimp per unit of water and significantly improving the economic benefits per production unit. In aquaculture, dissolved oxygen levels are a key factor affecting shrimp feeding behavior and overall health. Especially in high-density farming systems, increased dissolved oxygen levels can promote metabolism and enhance the vitality and appetite of Pacific white shrimp. However, high seedling density and large feeding amounts result in unabsorbed nutrients, large amounts of uneaten feed, and feces entering the water. Due to poor water purification capacity, uneaten feed, feces, and dead algae produce ammonia nitrogen, leading to a corresponding decrease in dissolved oxygen. A significant reduction in dissolved oxygen negatively impacts the growth of Litopenaeus vannamei. Since substances such as ammonia nitrogen, nitrite, and hydrogen sulfide easily accumulate under low-oxygen conditions and put stress on Litopenaeus vannamei, increasing dissolved oxygen levels can improve water quality and provide a healthier living environment for the shrimp.

[0004] Existing booster pumps increase dissolved oxygen in water by pumping water out and letting it fall back to the surface. However, the effectiveness of this oxygenation method is limited by factors such as pump flow rate and head, making it difficult to meet the oxygenation needs of high-density aquaculture or scenarios with poor water quality. Moreover, on cloudy or rainy days, the total amount of oxygen in the air is limited, causing booster pumps to consume a large amount of electricity to operate without guaranteeing an increase in dissolved oxygen concentration. In addition, Pacific white shrimp grow rapidly, and 2-3 harvests can be carried out per year. While drainage is generally not required during the farming process, the ponds must be drained after each harvest. Therefore, if the wastewater from aquaculture is not treated in a timely manner, it will pose a significant risk of pollution to the aquatic ecosystem. Currently, the most popular wastewater treatment model is represented by the "three ponds and two dams" approach, a "disseminated" treatment method. This model has advantages such as centralized treatment of contiguous ponds, but it also has three disadvantages: first, it requires planning 5-8% of the ponds for separate water treatment, reducing the production area; second, it requires investment in the construction and purchase of water treatment facilities, increasing production costs; and third, it requires long-term maintenance, increasing operation and management costs.

[0005] To address the above shortcomings, it is necessary to develop farming methods specifically for Litopenaeus vannamei to further improve the farming results. Summary of the Invention

[0006] Regarding the aforementioned technical problems in existing Litopenaeus vannamei (whiteleg shrimp) farming systems, where the effectiveness of booster pump aeration is limited by factors such as pump flow rate, head, and weather, and where off-site pond treatment of wastewater reduces production area and increases production and operational costs, the technical solution adopted by this invention addresses these issues as follows: An application of an enzymatically hydrolyzed composite carbon source in shrimp farming includes a pond equipped with a liquid oxygen supply assembly, an aeration pump assembly, and a feed dispensing device. The liquid oxygen supply assembly includes a liquid oxygen input device located on one side of the pond and a liquid oxygen output device located on the pond surface. The method includes the following steps: S100: Seawater is added to the pond, freshwater is used to adjust the salinity, the water is disinfected, and the colonies of bacteria occupy the space, forming bioflocs and introducing shrimp larvae and microalgae. S1. A liquid oxygen input device is installed on one side of the pond, with a preset flow rate of liquid oxygen to be delivered in a single operation and a preset start time for the aeration pump assembly. The liquid oxygen input device, the aeration pump assembly, and the liquid oxygen output device start simultaneously. S2. After the liquid oxygen input device, aeration pump assembly, and liquid oxygen output device have completed their preset operation time, the feed spreading device divides the feed and spreads it into the pond at a preset rotation speed. Steps S3, S1, and S2 should be repeated at least 3-5 times a day, using enzymatically hydrolyzed organic carbon sources.

[0007] Furthermore, in step S1, the liquid oxygen input device, in conjunction with the liquid oxygen output device, controls the amount of liquid oxygen input to add 0.05-0.1 grams of oxygen per cubic meter of water in the pond per day.

[0008] Furthermore, in step S2, the feed spreading device adopts a 12-hour uninterrupted feeding method, and the spreading is started for 2 minutes every 10 minutes.

[0009] Furthermore, the oxygenation pump assembly is provided in two parts and is diagonally positioned on the side away from the liquid oxygen supply assembly.

[0010] Furthermore, the feed dispensing device is equipped with a control unit and a photovoltaic cell module connected to the control unit. The control unit includes a remote control component for remotely controlling the movement of the feed dispensing device. The control unit adjusts the size of the feed according to the preset growth cycle of the whiteleg shrimp.

[0011] Furthermore, in step S1, the introduced bacterial colonies include Nitrosomonas, Denitrifying Pseudomonas, Bacillus subtilis, photosynthetic bacteria, and Lactobacillus acidophilus, and the introduced microalgae include Chlorella, Scenedesmus, Spirulina platensis, and Spirulina macrophylla.

[0012] Furthermore, in step S3, before adding the organic carbon source, soak it in a 2-5 mL / L lactic acid bacteria solution for 10 minutes. Within 30 days after the seedlings are released, supplement the organic carbon source daily at 50% of the feed amount, and add colonies every 5 days.

[0013] Furthermore, in step S3, during the middle and later stages of aquaculture, 30% of the feed amount is added as organic carbon material, and Bacillus subtilis solution and Lactobacillus acidophilus solution are sprinkled daily, and EM bacteria solution is sprinkled every 3-5 days.

[0014] Furthermore, in step S3, the organic carbon source includes bamboo powder, peanut shell powder, brown sugar, and tapioca flour. By mass, bamboo powder accounts for 10-20 parts, peanut shell powder accounts for 15-25 parts, brown sugar accounts for 30-50 parts, and tapioca flour accounts for 30-40 parts.

[0015] Furthermore, in step S3, the cassava flour is enzymatically hydrolyzed by α-amylase, saccharifying enzyme and protease, while the bamboo flour and peanut shell flour are enzymatically hydrolyzed by xylanase, β-glucosidase and manganese peroxidase.

[0016] The beneficial effects of this invention are as follows: 1. This invention artificially adds organic carbon sources to the aquaculture water to increase the number of heterotrophic bacteria. These microorganisms assimilate inorganic nitrogen, converting nitrogenous compounds such as ammonia nitrogen in the water into bacterial proteins, forming bioflocs that can be directly ingested by filter-feeding aquaculture organisms. This solves the problem of debris and feed retention in the aquaculture water, enabling feed reuse. It also purifies water, reduces water exchange, saves feed, improves the survival rate of aquaculture organisms, and increases yield. Compared to traditional manual feeding, automatic feeding offers significant advantages in saving labor, feed, and reducing the feed conversion ratio. It effectively shortens the aquaculture cycle, eliminates feed waste, reduces the gastrointestinal load on shrimp, improves the absorption rate of feed nutrients, and reduces water pollution.

[0017] 2. This invention, through the combination of an oxygenation pump assembly and a liquid oxygen supply assembly, can stably provide oxygen under any climatic conditions, unaffected by weather. The liquid oxygen supply method can provide a higher concentration of oxygen, thereby increasing the feeding frequency of Litopenaeus vannamei. Combined with a feed spreading device for feeding Litopenaeus vannamei, it improves farming efficiency.

[0018] 3. The aquaculture method of this invention helps maintain the dissolved oxygen level in the pond through timed and quantitative liquid oxygen supply, thereby promoting the healthy growth of Litopenaeus vannamei. Automatic feed dispensing reduces manual labor while improving feed utilization efficiency and feeding accuracy. Systematic management helps improve the predictability and controllability of the entire aquaculture process, enhancing aquaculture efficiency and product quality. Attached Figure Description

[0019] Figure 1 This is a schematic diagram illustrating the application of an enzymatically hydrolyzed composite carbon source of the present invention in shrimp farming.

[0020] Figure 2 This is a schematic diagram of another embodiment of the application of an enzymatically hydrolyzed composite carbon source in shrimp farming according to the present invention.

[0021] Figure 3 This is a schematic diagram of a liquid oxygen output device for the application of an enzymatically hydrolyzed composite carbon source in shrimp farming according to the present invention.

[0022] Figure 4 This is a schematic diagram of the internal structure (hidden liquid oxygen output housing) of the liquid oxygen output device of the present invention.

[0023] Figure 5 This is a three-dimensional structural diagram of the internal structure of the filter assembly of the liquid oxygen output device (hidden liquid oxygen output housing) of the present invention.

[0024] Figure 6 This is a partial three-dimensional structural diagram of the liquid oxygen output device (hidden liquid oxygen output housing) of the present invention.

[0025] Figure 7 This is a schematic diagram of the three-dimensional cross-sectional structure of the cleaning rod for the invention.

[0026] Figure 8 This is a partial three-dimensional structural diagram of the liquid oxygen output device (hidden liquid oxygen output housing) of the present invention.

[0027] Figure 9 This is a schematic diagram of the three-dimensional structure of the rotating rod of the invention.

[0028] Figure 10 This is a schematic diagram of the three-dimensional cross-sectional structure of the rotating shaft for the invention.

[0029] Figure 11 This is a schematic diagram of a feed spreading device for the application of an enzymatically hydrolyzed composite carbon source in shrimp farming according to the present invention.

[0030] Figure 12 This is a partial three-dimensional structural diagram of the feed spreading device (hidden feed spreading shell) of the present invention.

[0031] Figure 13 This is a partial three-dimensional structural cross-sectional view of the feed spreading device (hidden feed spreading shell) of the present invention.

[0032] Figure 14 This is a three-dimensional structural diagram of part of the feed spreading device (hidden feed spreading shell) of the present invention.

[0033] Figure 15 for Figure 14 Enlarged view of part A.

[0034] Figure 16 This is a three-dimensional structural diagram of part of the feed spreading device (hidden feed spreading shell) of the present invention.

[0035] Figure 17 This is a three-dimensional structural diagram of the oxygenation pump assembly of the present invention.

[0036] Figure 18 This is a schematic diagram of the fixed platform structure of the oxygen pump assembly of the present invention.

[0037] Figure 19 This is a schematic cross-sectional view of the fixed platform of the oxygen pump assembly of the present invention. Detailed Implementation

[0038] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0039] like Figures 1 to 19The invention illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming, comprising a pond 1. The pond 1 includes a liquid oxygen supply assembly 2, an aeration pump assembly 3, and a feed dispensing device 4. The liquid oxygen supply assembly 2 includes a liquid oxygen input device 5 located on one side of the pond 1, a liquid oxygen output device 6 located on the pond surface, and a transmission pipe 23 connecting the liquid oxygen input device 5 and the liquid oxygen output device 6. The aeration pump assembly 3 is located on the pond surface and works in conjunction with the liquid oxygen output device 6 to supply oxygen. The feed dispensing device 4 is used to add feed to the pond surface after oxygen supply is complete. This invention, through the cooperation of the aeration pump assembly and the liquid oxygen supply assembly, can stably provide oxygen under any climatic conditions, unaffected by weather. The liquid oxygen supply method can provide a higher concentration of oxygen, thereby increasing the feeding frequency of Litopenaeus vannamei. Combined with the feed dispensing device, this improves farming efficiency. This invention has the advantages of simple structure, convenient operation, good oxygenation effect, energy saving, and environmental protection, and is suitable for Litopenaeus vannamei farms of various sizes.

[0040] Furthermore, liquid oxygen is introduced from the liquid oxygen input device to the liquid oxygen output device via a transmission pipeline, and then dissolved into the pond. Compared to traditional air booster pumps, this method can more effectively meet the oxygenation needs of high-density aquaculture or poor water quality conditions. The liquid oxygen supply component is unaffected by changes in atmospheric oxygen content, thus ensuring effective improvement of dissolved oxygen levels in the water even on rainy days or other low-oxygen conditions. Simultaneously, the oxygenation pump component effectively improves oxygen transfer efficiency, thereby reducing energy consumption. The feed dispensing device delivers feed to the pond, allowing feeding to proceed only after ensuring adequate dissolved oxygen levels in the water. Sufficient oxygen levels enhance the activity and feeding appetite of Litopenaeus vannamei, contributing to improved feed utilization and feeding efficiency.

[0041] like Figures 1 to 10 The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The liquid oxygen input device 5 includes a vaporization component connected to a liquid oxygen cylinder, a flow meter, and a pressure regulating device. The liquid oxygen output device 6 includes a float 61 floating on the water surface, a liquid oxygen output housing 62 connected to the float 61 and partially extending into the water, a delivery pump 63 located on the liquid oxygen output housing 62 and connected to the transmission pipeline 23, and an aeration component 64 connected to the delivery pump 63. Specifically, the vaporization component connected to the liquid oxygen cylinder is used to vaporize liquid oxygen into gaseous oxygen; the flow meter is used to measure the flow rate of gaseous oxygen to control the oxygen input rate; and the pressure regulating device is used to regulate the gas pressure to ensure stable pressure and flow rate when the gas enters the transmission pipeline.

[0042] Furthermore, the float plate is located on the water surface to support the liquid oxygen output device and other components, and it floats and sinks with the change of water level, thereby driving the liquid oxygen output device and other components to move up and down. The liquid oxygen output shell is connected to the float plate and partially extends into the water, which protects the internal components of the liquid oxygen output device while restricting the direct release of liquid oxygen into the water, thereby reducing oxygen loss and improving oxygen utilization. The delivery pump is located inside the liquid oxygen output shell and is connected to the transmission pipeline to deliver the vaporized oxygen into the water. The aeration element outputs oxygen through micropores, nozzles, etc., increasing the contact area between oxygen and water molecules to increase the dissolved oxygen concentration in the water.

[0043] Optionally, in some embodiments, every 8 kg of liquid oxygen can be vaporized into 1 kg of oxygen and dissolved into the water by controlling the flow rate. Furthermore, the combined configuration of the liquid oxygen input device and the liquid oxygen output device integrates functions such as vaporization, flow regulation, pressure control, and oxygen delivery, which can achieve precise control and regulation of dissolved oxygen concentration in the water, thereby improving the oxygenation efficiency and water quality management level of the Litopenaeus vannamei farming system.

[0044] Specifically, the aeration element is connected to the delivery pump via the aeration element air pipe 641.

[0045] like Figures 1 to 10 The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The liquid oxygen output housing 62 includes a filter assembly 621 connected to the outside of the aeration element 64, and a rotating assembly 622 rotatable relative to the filter assembly 621. The rotating assembly 622 has a support frame 6221, a motor 6222 mounted on the support frame 6221, a rotating shaft 6223 connected to the motor 6222, and blades 6225 connected to the rotating shaft 6223. The filter assembly 621 has through-holes 6211 submerged in water. Furthermore, the filter assembly filters impurities and particulate matter from the water through multiple regularly spaced through-holes, ensuring that oxygen delivered to the water passes through the through-holes into the pond. This prevents large foreign objects from entering the liquid oxygen output housing, which could affect the contact and dissolution of oxygen with the water. Specifically, the motor can rotate the shaft forward or backward, causing the blades to rotate in either direction. When the shaft rotates, the blades help propel the water flow, increasing water mobility and thus improving the uniformity of oxygen distribution and dissolution efficiency.

[0046] Specifically, the through-holes allow water with low dissolved oxygen to enter the liquid oxygen output housing through the filtration assembly, or water with high dissolved oxygen to be transported from the liquid oxygen output housing to the pond through the filtration assembly. This water flow facilitates oxygen exchange, allowing oxygen to dissolve in the pond. The rotating assembly agitates the water and brings it into contact with oxygen. By rotating in both directions, it accelerates the movement of dissolved oxygen from the liquid oxygen output housing to the pond, preventing the water inside the liquid oxygen output housing from becoming saturated and unable to drain. It also allows water with low dissolved oxygen to enter the liquid oxygen output housing.

[0047] like Figures 1 to 10 The illustration shows the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The filter assembly 621 is equipped with an anti-clogging component 6212. The anti-clogging component 6212 includes a cleaning rod 62121 through which the rotating shaft 6223 passes and is connected to the rotating shaft 6223. The cleaning rod 62121 has a cleaning rod groove 621211 slidably connected to a cleaning block 621212 in the cleaning rod groove 621211, and an elastic reset member 621213 located between the inner wall of the cleaning rod groove 621211 and the cleaning block 621212. The cleaning block 621212 can extend into the through hole 6211. Specifically, the cleaning rod is connected to the rotating shaft and passes through the filter assembly. The cleaning block is located in the groove of the cleaning rod and is slidably connected within the groove. When the cleaning rod moves, the cleaning block also moves and can extend into the through-hole. It removes impurities attached to the edge of the through-hole by physically pushing outwards. An elastic reset element located between the inner wall of the cleaning rod groove and the cleaning block ensures that the cleaning block automatically returns to its original position after cleaning a single through-hole, ready for the next cleaning operation, thus reducing the need for manual intervention. When the cleaning block is not extended into the through-hole, the water can move smoothly on both sides of the through-hole. Specifically, the cleaning block is made of elastic material. The filter assembly is also made of elastic material, which reduces wear on the cleaning block and the filter assembly when the cleaning block extends into the through-hole during rotation.

[0048] Furthermore, some liquid oxygen output shells are typically placed in water for extended periods. When the liquid oxygen output shell machine stops operating, impurities in the water can easily clog the pores, thus affecting the dissolved oxygen effect. By setting up anti-clogging components, the pores of the filter components can be effectively prevented from being blocked, thus ensuring that oxygen is fully dissolved in the water, improving water quality, and providing a better environment for Litopenaeus vannamei shrimp farming.

[0049] Additionally, the filter assembly 621 includes a filter cylinder 62153, with a filter cylinder fixing sleeve 62156 fixedly connected to its outer wall. A first slider 62157 is slidably connected to the filter cylinder fixing sleeve 62156. The outer wall of the first slider 62157 is fixedly connected between a rotating rod 62155 and a blade 6225. The rotating rod 62155 has a slot 621551. The filter cylinder 62153 has a filter cylinder groove 621531 for the first slider 62157 to slide. Specifically, the filter cylinder fixing sleeve is circular, allowing the first slider to rotate in the filter cylinder groove, thus driving the blade to rotate. Furthermore, a moving rod 621521 is slidably connected to the rotating shaft 6223. One end of a spring 621524 is fixedly connected to the outer wall of the moving rod 621521, and the other end of the spring 621524 is fixedly connected to the inner wall of the rotating shaft 6223. A locking rod 621525 is fixedly connected to the end of the moving rod 621521. A first sliding sleeve 621522 is fixedly connected to the outer wall of the moving rod 621521. The first sliding sleeve 621522 is slidably connected to the rotating shaft 6223, and a threaded sleeve 621523 is rotatably connected to the first sliding sleeve 621522.

[0050] Furthermore, when the threaded sleeve is threadedly connected to the rotating shaft, the first sliding sleeve, which is rotatably connected to the threaded sleeve, drives the moving rod to slide downward. At the same time, the spring undergoes elastic deformation. When the moving rod slides downward, the locking rod fixedly connected to the bottom of the moving rod will be locked into the slot, thus completing the locking with the rotating rod. Then the motor is started, and the blades will rotate under the action of the rotating rod, thereby accelerating the oxygen and water fusion efficiency.

[0051] like Figures 1 to 10 The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The liquid oxygen output shell 62 is provided in two sets, and a buffer distance is provided between the aeration element 64 and the liquid surface. Furthermore, by providing two sets of liquid oxygen output shells, the stability and reliability of the system can be increased. Even if one set fails or requires maintenance, the other set can continue to operate, ensuring that the oxygen supply in the water is not affected. The buffer distance ensures that there is sufficient space between the aeration element and the liquid surface. After oxygen is released from the aeration element, it first contacts the water boundary layer in the air, which to some extent increases the oxygen dissolution time, helping to improve the oxygen dissolution efficiency in the water and allowing oxygen to contact the water more fully, thus improving the oxygen dissolution efficiency. The buffer distance, formed by the floating plate and the liquid oxygen output shell partially extending into the water, can better adapt to changes in water level, maintaining a stable operating state. Regardless of whether the liquid level rises or falls, it can maintain an appropriate distance between the aeration element and the water surface.

[0052] Furthermore, two symmetrical floats are arranged, each with a control valve on its liquid oxygen output housing. The height of the aeration element is higher than the top plane of the float, ensuring that the gas generated by the aeration element does not come into contact with the liquid surface. Since the liquid oxygen is transported under high pressure after vaporization and its temperature is lower than the ambient temperature, its solubility will decrease if it is directly introduced into the water after vaporization. If the liquid oxygen is not completely vaporized and directly introduced into the water, the heat released during vaporization will rapidly heat the water, easily causing the water surface to boil rapidly. Excessive temperature difference can adversely affect the whiteleg shrimp. Moreover, the gas will escape rapidly, preventing oxygen from mixing with the water. The buffer distance prevents water from flowing back into the aeration element and avoids the safety hazards caused by water being directly sucked into the delivery pump or even the delivery pipeline and coming into contact with incompletely vaporized liquid oxygen. Furthermore, bioflocs require high dissolved oxygen during their formation stage to allow the microorganisms within the flocs to grow and reproduce rapidly in a suitable environment, while also requiring flowing water to prevent the flocs from settling. The filter assembly of the present invention can drive the liquid flow around the aeration element, which can improve the diffusion of dissolved oxygen in the water. At the same time, the process of the blades stirring the water is not too violent. Stirring the water can keep the bioflocs in a suspended state, reduce the formation of sediment dead zones, and the process of liquid oxygen dissolution is gentle, which can make the bioflocs aggregate. It can avoid the flocs in the water being overly dispersed, which would cause the flocs to break easily and fail to combine and grow.

[0053] like Figures 11 to 16The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The feed dispensing device 4 includes a feed dispensing shell 41 and a base 42 connected to the bottom of the feed dispensing shell 41. The uppermost layer of the base 42 is provided with a feeding component 421, a cutting component 422 located below the feeding component 421, and a blowing component 423 connected to the cutting component 422. The feed dispensing shell 41 has an opening 44 on its outer side. The blowing component 423 is used to blow the feed cut by the cutting component 422 to the outside of the opening 44. Specifically, the feeding component is funnel-shaped, wider at the top and narrower at the bottom, used to introduce feed into the device for subsequent processing. The cutting component is located below the feeding component and is used to cut and process the feed, making it more suitable for dispensing. At the same time, it cuts the feed into sizes suitable for consumption by Litopenaeus vannamei, ensuring that the feed is easier to digest and absorb, and improving feed utilization. The blowing component is connected to the cutting component, generating a directional airflow to quickly and evenly deliver the cut feed particles to the outside of the feed dispensing shell opening, scattering them across the pond surface. This allows the Pacific white shrimp to consume food more widely, preventing feed from piling up in one place. The feed dispensing device enables automated feed dispensing, improving feed delivery efficiency, reducing the need for manual operation, and ensuring uniform feed distribution. It effectively disperses feed within a designated area, ensuring that aquatic organisms can obtain sufficient food in a timely manner, thereby guaranteeing their growth and health. Furthermore, the feed dispensing device 4 is equipped with a photovoltaic module and a feed dispensing control component. The photovoltaic module absorbs and stores electrical energy from sunlight and releases it, ensuring the continuous operation of the feed dispensing device. The feed dispensing control component controls the dispensing time, effectively controlling the feeding time and frequency of the shrimp.

[0054] like Figures 11 to 16The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. A filter plate 424 is positioned between the cutting component 422 and the blowing component 423. A rotatable striking component 425, in contact with the filter plate 424, is connected to the lower side of the filter plate 424. A conveying ramp 426 is located on the lower side of the filter plate 424, with a conveying area 427 at its bottom. This conveying area 427 is located between the blowing component 423 and the feed dispensing shell opening 44. Furthermore, the filter plate, situated between the cutting and blowing components, effectively filters out impurities and larger particles from the feed. Only appropriately sized, cut and processed feed particles can enter the conveying ramp, ensuring the quality of the dispensed feed. The striking component, rotatable and in contact with the filter plate, is located below it. When the striking component approaches the filter plate, it performs a striking motion to help separate feed particles adhering to the filter plate due to electrostatic force or pressure, maintaining the filter plate's patency. The conveying ramp guides the feed towards the appropriate position. The bottom of the conveying ramp has a conveying area to ensure that the feed can smoothly reach the space between the blowing assembly and the feed spreading shell opening, facilitating feed spreading operations. The conveying ramp not only simplifies the feed conveying process but also improves feed conveying efficiency.

[0055] Furthermore, a crushing bin 413 is provided inside the feed spreading shell 41, and a cutting assembly 422 is located inside the crushing bin 413. A fixing plate 418 is fixedly connected below the fixing plate 418, and the cutting assembly 422 is located below the first motor 417. The cutting assembly 422 includes a first rotating wheel 42291, which is fixedly connected to the output shaft of the first motor 417. A round rod 422917 is fixedly connected below the first rotating wheel 42291, and crushing rods 422918 are fixedly connected around the outer wall of the round rod 422917. A striking assembly 425 is also included. The structure includes the following components, specifically: A first rotating wheel 42291 is drivenly connected to a first conveyor belt 42292; a second rotating wheel 42293 is drivenly connected to the end of the first conveyor belt 42292 away from the first rotating wheel 42291; a first rotating shaft 42294 is fixedly connected to the second rotating wheel 42293; a first fixing plate 42295 is rotatably connected to the first rotating shaft 42294, and the first fixing plate 42295 is fixedly connected to the outer wall of the feed spreading shell 41; a second fixing plate 42296 is fixedly connected below the first fixing plate 42295; and a second rotating shaft 42294 is fixedly connected below the first rotating shaft 42294. A second bevel gear 422910 meshes with a first bevel gear 42299. The first bevel gear 42299 is fixedly connected to a second rotating shaft 42298, and the second rotating shaft 42298 is rotatably connected to a second fixed plate 42296. Further, a rotating block 422911 is fixedly connected to the end of the second rotating shaft 42298 away from the second fixed plate 42296. The rotating block 422911 is fixedly connected to a cylinder 422921. The cylinder 422921 is slidably connected to a connecting rod 422912. The connecting rod 422912 is fixedly connected to a sector-shaped... Block 422915, the sector block 422915 is rotatably connected to a fixing plate three 422916, the fixing plate three 422916 is fixedly connected to the lower part of the fixing plate one 42295, multiple sets of toothed blocks are fixedly connected to the lower part of the sector block 422915, the multiple sets of toothed blocks are meshed with gears 422913, gears 422913 are fixedly connected to a rotating rod 422914, the rotating rod 422914 penetrates the inner wall of the feed spreading shell 41, and a striking component 425, such as a hammer 4250, is fixedly connected to the outer wall of the end of the rotating rod 422914 away from the gear 422913.

[0056] Furthermore, the filter plate is equipped with mesh holes. The feed to be cut is loaded through the feed inlet of the feeding assembly. Starting the motor drives the rotation of the first rotating wheel and the round rod. The rotation of the round rod drives the crushing rod to crush the feed in the crushing bin. The crushed feed falls through the filter plate onto the conveyor ramp below, and then falls through the conveyor ramp to the opening of the feed spreading shell, where it is blown out by the blowing assembly 423. Driven by the first conveyor belt, the rotation of the first rotating wheel drives the rotation of the second rotating wheel, which in turn drives the rotation of the first rotating shaft, which in turn drives the rotation of the second bevel gear. Wheel 2 drives the rotation of bevel gear 1, which in turn drives the rotation of shaft 2, which in turn drives the rotation of the rotating block and cylinder. This causes the connecting rod and sector block to swing back and forth. Through the action of the toothed block below the sector block and the gear meshing with it, the back and forth swing of the sector block drives the gear to swing back and forth, which in turn drives the rotating rod to rotate back and forth. This, in turn, drives the hammer to move back and forth to strike and vibrate the area below the filter plate, making it easier to knock down the feed that is blocked in the filter plate mesh, which falls onto the inclined plate below and then onto the dispensing port for discharge.

[0057] Furthermore, the feed spreading device loads the feed to be cut into the crushing bin through the feed inlet, starts the motor to drive the rotation of the rotating wheel and the round rod, and the rotation of the round rod will drive the crushing rod to crush the feed in the crushing bin. The crushed feed will fall through the filter plate onto the inclined plate below, and then fall through the inclined plate to the spreading port for discharge, thus spreading the feed. The feed spreading device, driven by conveyor belt one, causes the first rotating wheel to rotate, which in turn drives the first rotating shaft to rotate, which in turn drives the second bevel gear to rotate. The second bevel gear then drives the first bevel gear to rotate, which in turn drives the second rotating shaft to rotate, which in turn drives the rotating block and the cylinder to rotate. This causes the connecting rod and the sector block to swing back and forth. Through the action of the toothed block below the sector block and the gear meshing with it, the back and forth swing of the sector block drives the gear to swing back and forth, which in turn drives the rotating rod to rotate back and forth. This in turn drives the hammer to move back and forth to strike and vibrate the area below the filter plate, making it easier to knock down the feed that is blocking the filter plate mesh, which falls onto the inclined plate below and then onto the spreading port for discharge.

[0058] Specifically, the conveying area 427 includes a tilting channel 4271 connected to the outside of the crushing hopper, and a blowing port 4272 connected to the opening of the feed spreading shell. The tilting channel and the blowing port are connected. The blowing port is inclined from bottom to top, which allows the feed to move upward under the airflow of the blowing assembly and to drift to a farther place. In addition, the blowing assembly includes a blower, which is fixed to the underside of the base plate or the inside of the feed spreading shell. Furthermore, the upper side of the feed spreading shell is provided with a cover to prevent moisture from entering the feeding assembly and affecting the dryness of the feed.

[0059] Furthermore, the feeding assembly includes a feeding hopper 4201 that is larger at the top and smaller at the bottom, a movable feeding plate 4202 located below the feeding hopper, a feeding trough 4207 located below the feeding plate, a feeding limiting rod 4203 connected to the feeding plate and fixed inside the feed spreading shell, and a feeding motor 4204 connected to the feeding plate and fixed at one end inside the feed spreading shell. A feeding movable rod 4205 is connected between the feeding motor and the feeding plate. The feeding plate is provided with a relief groove 4206 for limiting the feeding limiting rod to move forward and backward. When the feeding motor starts, the feeding movable rod pushes the feeding plate forward to the closed position. The limiting rod and the relief groove restrict the feeding plate from continuing to move. The feeding plate is located between the feeding trough and the feeding hopper, and feeding cannot continue at this time. When the feeding lever pushes the feeding plate backward to the open position, the limit lever and the clearance groove restrict the feeding plate from moving further, and the feeding plate moves away from the space between the feeding trough and the feeding hopper, allowing for continuous feeding. The feeding motor can be a stepper motor, a lead screw motor, etc.

[0060] Additionally, the base is connected to a first float 4200 and a second float 4208, as well as a drive assembly, for supporting the entire feed dispensing shell and driving the feed dispensing device to move on the water surface. The drive assembly includes a drive motor and a stirring paddle 420. Furthermore, the blowing assembly includes four blowers located at the four corners of the conveying area. The conveying ramp is pyramidal in shape, comprising four ramps corresponding one-to-one with the conveying area. The feed dispensing shell also has four openings, allowing feed to be simultaneously dispensed from all four sides of the shell, improving dispensing efficiency.

[0061] Optionally, in some embodiments, the feed distributing device further includes a control unit and a photovoltaic cell assembly connected to the control unit. The feed distributing device can charge the photovoltaic cell assembly under sunlight, improving its ease of use. Additionally, the control unit includes a remote control component, allowing users to connect via wireless networks, Bluetooth, infrared, etc., and remotely control the movement of the feed distributing device using control tools such as computers, mobile phones, or remote controls. Furthermore, the control unit can adjust the feed size according to a preset growth cycle of the Litopenaeus vannamei. Specifically, when the Litopenaeus vannamei is in the larval stage, the control unit increases the rotation speed of the cutting component, further reducing the feed size. When the Litopenaeus vannamei is in the adult stage, the control unit slows down or even stops the rotation of the cutting component, ensuring the feed size is suitable for the Litopenaeus vannamei's consumption and preventing excessively fine feed from dissolving directly in the pond, causing environmental pollution and waste.

[0062] like Figures 17 to 19The illustration illustrates the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The aeration pump assembly 3 includes a support 31 fixed at one end to the pond, a booster pump located on the support 31, multiple buoyancy components 32 symmetrically arranged on the outside of the support 31, and an anti-tipping assembly 33 connecting the support 31 and the buoyancy components 32. The anti-tipping assembly 33 includes a sliding component 331 for the buoyancy components 32 to move vertically, and a hinged component 332 connecting the sliding component 331 and the buoyancy components 32. When the buoyancy components 32 move upward along the sliding component 331, the hinged component 332 drives the buoyancy components 32 towards the center of the support 31. Furthermore, fixing one end of the support in the pond provides a structural foundation. The support provides a mounting position for the booster pump and other components. The booster pump, located on the support, draws in oxygen from the external environment, pressurizes it, and delivers it to the pond to increase the oxygen content in the water. The buoyancy assembly, consisting of multiple symmetrically arranged components on the outside of the support, provides buoyancy to support the entire oxygen booster system and ensure it floats on the water. A sliding assembly allows the buoyancy assembly to move up and down as needed to adapt to changes in pond water level. A hinged assembly connects to the sliding and buoyancy assemblies; when the water level changes, the hinged assembly, under the influence of buoyancy, drives the buoyancy assembly to switch between an extended and retracted state relative to the support. Since one end of the support is fixed to the pond, when the water level rises, as the buoyancy assembly moves upward along the sliding assembly, the hinged assembly drives the buoyancy assembly towards the center of the support, thus maintaining the system's balance and stability and preventing capsizing or other adverse conditions.

[0063] like Figures 17 to 19The illustration shows the application of an enzymatically hydrolyzed composite carbon source in shrimp farming. The support member 31 includes an upper panel 311 fixed at one end to the pond, and a fixed platform 334 connected to a hinge assembly 332 for placing a booster pump. A support rod 312 is fixedly connected to the bottom surface of the upper panel 311. A sliding groove 3121 is formed on the surface of the support rod 312. A sliding sleeve 313 is provided between the inner wall of the sliding groove 3121 and the surface of the support rod 312. The hinge assembly 312... The buoyancy assembly 32 includes a long arc rod 3321 and a short arc rod 3322. The surface of the sliding sleeve 313 is hinged to one end of the long arc rod 3321 via a coil spring, and the other end of the long arc rod 3321 is hinged to one end of the short arc rod 3322. The other end of the short arc rod 3322 is hinged to the surface of the fixed platform 334. A fixing ring 33211 is fixedly connected to the surface of the long arc rod 3321, and the buoyancy assembly 32 is a float fixedly connected inside the fixing ring 33211. Further, one end of the upper panel is fixed to the pond, providing a basic support structure for the entire aerator pump assembly. The fixed platform is connected to the hinged assembly and is used to support and install the booster pump, ensuring that the booster pump can still operate stably in a floating state. The support rod, as a component fixedly connected below the bottom surface of the upper panel, has a sliding groove on its surface to guide and support the movement of the buoyancy assembly. A sliding sleeve is installed between the inner wall of the sliding groove and the surface of the support rod, allowing the sliding sleeve to slide up and down within the sliding groove to adapt to changes in the height of the buoyancy assembly. The sliding sleeve and the long arc rod are hinged by a coil spring, allowing them to move up and down with the buoyancy component. Force is transmitted through the hinge assembly, causing the buoyancy component to move closer to the center of the support member when it rises. Furthermore, the support rod 312 is V-shaped from top to bottom, giving the hinge assembly a certain deformation space. When the hinge assembly moves downwards, it is in an unfolded state; when it moves upwards, it is in a retracted state.

[0064] Furthermore, one end of the long arc rod is hinged to the sliding sleeve via a coil spring, and the other end is hinged to the short arc rod, acting as a connecting rod to convert the lifting and lowering motion of the buoyancy assembly into a force that pushes the buoyancy assembly towards the center. The other end of the short arc rod is hinged to the long arc rod and also hinged to the surface of the fixed platform. By changing the angle at the hinge points at both ends, the position of the buoyancy assembly is driven to change. The buoyancy assembly consists of floats fixed inside the fixed ring. Due to their own buoyancy characteristics, the floats rise and fall with changes in water level. Through the linkage effect of the hinged components, the stability of the entire aerator pump assembly is maintained at all times, preventing it from capsizing.

[0065] Additionally, the fixed platform 334 includes a platform shell 334201, a rotating rod 334203, and a platform fixing plate 334205. A limit block 334202 is fixedly connected to the upper surface of the platform shell 334201. The interior of the platform shell 334201 is rotatably connected to the upper surface of the rotating rod 334203. One end of a spring 334204 is fixedly connected to the surface of the platform fixing plate 334205, and the surface of the platform fixing plate 334205 is fixedly connected to the interior of the platform shell 334201. A fixing buckle 334206 and a toggle lever 334207 are fixedly connected to the upper surface of the rotating rod 334203. Specifically, by using a long arc rod, when it rains, the water level rises, causing the float to push the sliding sleeve to slide on the surface of the support rod. The sliding sleeve is guided by the sliding groove, causing it to rotate, which in turn drives the long arc rod to rotate. This causes the long arc rod and the short arc rod to fold together, thus rolling the float towards the center and reducing the impact area between the float and the water waves. This gives the aerator pump assembly better resistance to wind and waves, and greatly reduces the instability of the center of gravity caused by excessive lifting.

[0066] Furthermore, the oxygen pump assembly, through its fixed platform, allows for easy installation of the oxygen pump. When the oxygen pump needs to be installed, the lever is turned to rotate the retaining clips, opening them. When the oxygen pump is placed inside, the lever is released, and the rotating rod rotates under the action of the spring, causing the retaining clips to engage with the slots at the bottom of the oxygen pump, thus securing the oxygen pump. This makes both installation and removal of the oxygen pump convenient.

[0067] A method for using an enzymatically hydrolyzed complex carbon source in shrimp farming includes the following steps: S1. A liquid oxygen input device 5 is installed on one side of the pond, with a preset flow rate of liquid oxygen to be delivered per cycle and a preset start-up time for the aeration pump assembly 3. The liquid oxygen input device 5, the aeration pump assembly 3, and the liquid oxygen output device 6 start simultaneously. Specifically, the liquid oxygen input device, which includes a vaporization component, a flow meter, and a pressure regulating device, is installed on one side of the pond to ensure that liquid oxygen can be stably and safely converted into gaseous oxygen and to precisely control its flow rate and pressure. The required flow rate of liquid oxygen to be delivered per cycle is preset based on the pond size, stocking density, and water quality. Simultaneously, the start-up time of the aeration pump assembly is set to ensure that the pressure and distribution range of oxygen are increased through the pressurization component while liquid oxygen is being input. At the preset time, the liquid oxygen input device, the aeration pump assembly, and the liquid oxygen output device start simultaneously to ensure the increase and maintenance of oxygen content in the water. The liquid oxygen input device, aeration pump assembly, and liquid oxygen output device are activated simultaneously to ensure that oxygen is rapidly and evenly distributed throughout the pond, providing an ample oxygen supply for the Pacific white shrimp. The liquid oxygen input device, in conjunction with the liquid oxygen output device, controls the input volume to add 0.05-0.1 grams of oxygen per cubic meter of water per day to the pond.

[0068] After the preset time has elapsed for the liquid oxygen input device 5, the oxygenation pump assembly 3, and the liquid oxygen output device 6, the feed spreading device 4 divides the feed and spreads it into the pond at a preset speed. After the preset time has elapsed following the start of operation of the liquid oxygen input device and the oxygenation pump assembly, the feed spreading device is activated. The feed spreading device begins operation after the preset time for liquid oxygen input and oxygen pressurization has elapsed. This device, through the coordinated action of the feeding assembly, cutting assembly, and blowing assembly, divides the feed at a preset speed and spreads it evenly into the pond through the blowing assembly. The uniformity of feed spreading is ensured by adjusting the speed of the feed spreading device and the airflow of the blowing assembly, guaranteeing that the feed is evenly spread to all corners of the pond to meet the feeding needs of the Pacific white shrimp. The feed is divided and spread into the pond according to the preset speed to meet the feeding needs of aquatic organisms.

[0069] Steps S3, S1, and S2 are repeated 3-5 times daily. The frequency can be adjusted based on the growth stage, feeding habits, and weather conditions of the Litopenaeus vannamei. During these repeated operations, close monitoring of the pond's water quality, shrimp growth, and feed consumption is crucial. Timely adjustments and optimizations are necessary to ensure efficient operation of the aquaculture system, maintain stable oxygen levels in the water, and meet the feed requirements of aquatic organisms. This ensures good water quality within the aquaculture system, promoting the growth and health of aquatic life. By implementing these steps, a high-efficiency Litopenaeus vannamei aquaculture system can be effectively managed and maintained, improving aquaculture efficiency and yield. The aquaculture method of this invention, through timed and quantitative liquid oxygen supply, helps maintain dissolved oxygen levels in the pond, thereby promoting the healthy growth of aquatic animals. Automatic feed dispensing reduces manual labor while improving feed utilization efficiency and feeding accuracy. Systematic management helps improve the predictability and controllability of the entire aquaculture process, enhancing aquaculture efficiency and product quality.

[0070] Specifically, shrimp pond feeding is primarily done during the day. Traditional manual feeding involves feeding three times a day: 7:00 AM, 12:00 PM, and 6:00 PM. Farmers use a workboat to carry feed around the pond and scatter it all at once. The disadvantages are that it is time-consuming and labor-intensive, and since the feed is only given once, shrimp feed slowly, typically taking 1.5-2 hours to finish eating. During this time, some nutrients in the feed are converted into pollutants and released into the water. The long soaking time also reduces the feeding effect, and uneaten feed is not only wasteful but also a major source of water pollution. The new method uses a 12-hour continuous feeding method from 7:00 AM to 7:00 PM. The feed spreading device is equipped with photovoltaic components and feed spreading control components, distributing feed every few minutes. After each round of feeding, the shrimp are promptly cleaned and eaten, fundamentally solving the drawbacks of wasteful manual feeding and reducing feed pollution to the water. Furthermore, Pacific white shrimp grow rapidly, with 2-3 harvests per year. While drainage is generally not required during the farming process, the ponds must be drained after each harvest. Therefore, if the wastewater is not treated promptly, it poses a significant pollution risk to the aquatic ecosystem. Currently, the most popular wastewater treatment model is represented by the "three ponds and two dams" approach, a "disseminated" treatment method. This model has advantages such as centralized, contiguous pond treatment, but it also has three disadvantages: first, it requires planning 5-8% of the ponds for separate water treatment, reducing the production area; second, it requires investment in the construction and equipment of water treatment facilities, increasing production costs; and third, it requires long-term maintenance, increasing operation and management costs.

[0071] To address the above problems, this invention also provides a solution for treating wastewater using bioflocs. Specifically, seawater is added to the aquaculture pond, and the salinity is adjusted to approximately 0.5% to 2.5% using freshwater. The water temperature is controlled at 20-30℃, and then 20-30g / m³ of bleaching powder is added.3 Disinfect the water with 100 kg / mu of quicklime. After aeration for 1-2 days until there is no residual chlorine in the water, since the removal of ammonia nitrogen and nitrite nitrogen by bioflocs has a certain lag, the bioflocs can be cultivated in advance before the seedlings are released.

[0072] Specifically, seven days before stocking, EM bacteria and amino acid fertilizer paste are added to the pond to utilize the colony's biological colony-forming properties. Every morning, Nitrifying Monoclonal bacteria, Denitrifying Pseudomonas, Bacillus subtilis, photosynthetic bacteria, and Lactobacillus acidophilus are applied to the entire pond, with the addition amount approximately 7.5 × 10⁻⁶ for Nitrifying Monoclonal bacteria. 6 cfu·L -1 Denitrifying Pseudomonas aeruginosa 6.0 × 10 6 cfu·L -1、 Bacillus subtilis 7.0 × 10 6 cfu·L -1 3.0 × 10⁻⁶ photosynthetic bacteria 8 cfu·L -1 Lactobacillus acidophilus 1.0 × 10 6 cfu·L -1 Simultaneously, add 1 kg of brown sugar per mu (approximately 0.067 hectares) and sprinkle it throughout the pond. After 5-7 consecutive days of application, flocculent particles will appear in the water, and bioflocs will begin to form. At this time, stock the shrimp fry, placing them in the aforementioned rearing pond at a stocking density of 100-200 shrimp per m². 3 The introduction of microalgae brought the algae concentration in the water to 5 × 10⁻⁶. 4 CFU / mL. Furthermore, the microalgae used were *Chlorella vulgaris*, *Scenedesmus*, *Arthrophagus platensis*, and *Arthrophagus macrocarpa*. Continuous aeration for 15 days was maintained to achieve a biofloc concentration of over 5 ml / L (sedimentation volume in half an hour) and keep it stable.

[0073] Specifically, *Chlorella* and *Scenedesmus* are high-quality single-cell protein sources that can be directly used as feed for shrimp larvae. They can also provide food for small aquatic organisms (such as rotifers, cladocerans, and copepods), which in turn serve as natural food sources for shrimp, thus improving the self-sufficiency of the entire aquaculture system. *Spiralella platensis* and *Spiralella maxima* are rich in protein, vitamins, and minerals, and can significantly improve the growth performance and health of shrimp in the biofloc system during the later stages of aquaculture. The presence of microalgae acts as a "binder" for bioflocs; their secreted extracellular polysaccharides and other substances help flocculate particles, enhancing the physical stability of bioflocs, making them more compact and less prone to dispersion. Microalgae produce oxygen through photosynthesis, increasing dissolved oxygen levels in the water and helping to improve water quality. *Chlorella* and *Scenedesmus* absorb excess nitrogen, phosphorus, and other nutrients, helping to reduce eutrophication, prevent algal blooms, and maintain water cleanliness. Before adding the carbon source, soak the microalgae in a 2-5 mL / L lactic acid bacteria solution for 10 minutes. Within 30 days after seedling release, supplement with organic carbon at 50% of the daily feed intake, and simultaneously introduce Nitrosomonas, Denitrifying Pseudomonas, Bacillus subtilis, photosynthetic bacteria, and Lactobacillus acidophilus every 5 days, with the addition amount being approximately 7.5 × 10⁻⁶ for Nitrosomonas. 6 cfu·L -1 Denitrifying Pseudomonas aeruginosa 6.0 × 10 6 cfu·L -1、 Bacillus subtilis 7.0 × 10 6 cfu·L -1 3.0 × 10⁻⁶ photosynthetic bacteria 8 cfu·L -1 Lactobacillus acidophilus 1.0 × 10 6 cfu·L -1 In the later stages of aquaculture, 30% of the feed should be supplemented with organic carbon. During the aquaculture process, potassium persulfate should be used every 10-20 days to improve the bottom sediment. The pH of the water should be adjusted to between 7.1 and 8.1, and the total alkalinity to between 150 mg and 300 mg / L by adding sodium carbonate solution and quicklime water. In the later stages of aquaculture, the concentration of bioflocs in the water should be kept stable at 15-30 mL / L. No water changes are performed throughout the entire aquaculture process; only a small amount of fresh water is used to replenish water lost through evaporation. In the later stages of aquaculture, a daily concentration of 7.0 × 10⁻⁶ is applied. 6 cfu·L -1 Bacillus subtilis solution, 500 g / mu, concentration 1.0 × 10⁻⁶. 6 cfu·L -1 Apply 800 grams of Lactobacillus acidophilus solution per acre, and spray 10 kilograms of EM bacteria solution per acre every 3-5 days to improve the stability of algae. Turn on the aerator within 1 hour after spraying.

[0074] Specifically, *Nitrosomonas* can oxidize ammonia nitrogen to nitrite. While *Nitrosomonas* themselves do not directly participate in biofloc formation, they help improve water quality by reducing ammonia nitrogen levels, providing a more suitable growth environment for bioflocs. *Denitrifying Pseudomonas* possess the characteristics of heterotrophic nitrification, aerobic denitrification, and highly efficient nitrogen removal. They can undergo nitrification under aerobic conditions, converting ammonia nitrogen to nitrite, and then to nitrate; under anaerobic conditions, they can undergo denitrification, reducing nitrate to nitrogen gas, thus removing nitrogen from the water. This helps reduce nitrogen load in the water, decreases nitrogen accumulation in bioflocs, and prevents their excessive growth. *Bacillus subtilis* is an important component of bioflocs. They can secrete large amounts of extracellular polymers such as polyglutamic acid, which contribute to the formation and stabilization of bioflocs. Furthermore, *Bacillus subtilis* can improve the microbial community structure within bioflocs, enhancing the stability and efficiency of the entire system. Photosynthetic bacteria, especially the phototrophic organic heterotrophic Rhodospirillumaceae family, can synthesize organic nitrogen compounds from small organic molecules and inorganic nitrogen, thus degrading ammonia nitrogen and nitrite. In biofloc systems, they can reduce nitrogen content in this way while producing organic matter to provide a carbon source for other microorganisms. Lactobacillus acidophilus indirectly promotes the formation and stabilization of bioflocs mainly by improving water quality and enhancing the immunity of farmed animals. EM bacteria, composed of various beneficial microorganisms, have broad biological activities and functions, including promoting shrimp growth, improving feed utilization, enhancing immune function, and eliminating pollution. They are convenient and readily available; however, their enhancement effect is not as targeted as the combination of the above-mentioned multiple bacterial species.

[0075] During the growth of bioflocs, heterotrophic bacteria consume large amounts of alkalinity and produce significant amounts of CO2 while synthesizing bioflocs using ammonia nitrogen compounds, leading to a decrease in the pH of the aquaculture system. Furthermore, nitrifying bacteria produce acidic substances while utilizing ammonia nitrogen compounds, further lowering the pH of the aquaculture water. Additionally, the high density of organisms in the aquaculture system results in substantial CO2 production in the later stages of cultivation, and since the water is not replaced during biofloc cultivation, the pH of the aquaculture water tends to decrease. Microalgae in the aquatic body can utilize the CO2 produced by bacteria and larvae for photosynthesis, while bacteria utilize the C2 produced by microalgae to degrade carbon-containing substances. When bacteria dominate the aquatic environment, a large amount of CO2 enters the water, combining with water molecules to form HCO3-. -This affects the pH and alkalinity of the water. A large number of microalgae in the water carry out photosynthesis, which can absorb nutrients such as dissolved nitrogen. Microalgae can fix nitrogen in the water and provide food for larvae, thereby improving the utilization rate of nitrogen. Meanwhile, by reducing the nitrogen content and utilizing organic carbon sources, this invention artificially adds organic carbon to the aquaculture water, adjusting the carbon-to-nitrogen ratio (C / N) and increasing the number of heterotrophic bacteria. Microorganisms assimilate inorganic nitrogen, converting nitrogenous compounds such as ammonia nitrogen in the water into bacterial proteins, forming bioflocs that can be directly ingested by filter-feeding aquaculture organisms. This solves the problem of debris and feed retention in the aquaculture water, enabling feed reuse and purifying water quality, reducing water exchange, saving feed, improving the survival rate of aquaculture organisms, and increasing yield. Compared to traditional manual feeding, automatic feeding has significant advantages in saving labor, saving feed, and reducing the feed conversion ratio. The application of 12-hour continuous automatic precision feeding technology is particularly significant, effectively shortening the aquaculture cycle, eliminating feed waste, reducing the gastrointestinal load on shrimp, improving the absorption rate of feed nutrients, and reducing water pollution.

[0076] The carbon sources used are bamboo powder, peanut shell powder, brown sugar, and cassava flour. The type of organic carbon source affects the rate of biofloc formation and the microbial community structure. Brown sugar can rapidly form a large-scale system in aquaculture water, but it increases aquaculture costs in actual production. Poorly soluble carbon sources such as peanut shell powder and bamboo powder are rich in lignin, cellulose, and hemicellulose. Cassava flour contains a large amount of starch, is inexpensive, and forms a relatively stable system, but the system requires reaction time to form a large scale. The mixing ratio of bamboo powder, peanut shell powder, brown sugar, and cassava flour, by mass, is 10-20 parts bamboo powder, 15-25 parts peanut shell powder, 30-50 parts brown sugar, and 30-40 parts cassava flour.

[0077] Furthermore, to improve the conversion efficiency of bamboo powder, peanut shell powder, and cassava powder, enzymatic hydrolysis is necessary. Enzymatic hydrolysis helps release lignin, cellulose, and hemicellulose from bamboo powder and peanut shell powder. By helping to break down the rigid structure of lignin, it increases the release rate of total sugars, making them more readily utilized by microorganisms and thus accelerating the formation of bioflocs. Enzymatic hydrolysis of cassava powder can efficiently break down starch into simple sugars, providing a rapid energy source for microorganisms. The organic matter in the hydrolysis products can act as a gelling agent, promoting adhesion and aggregation between microorganisms, forming denser and more stable bioflocs, which helps capture and settle suspended particles, thereby improving water quality. Brown sugar can be rapidly metabolized by microorganisms, accelerating the growth of microbial communities, especially bacteria. Specifically, the preparation method of enzymatically hydrolyzed cassava powder is as follows: cassava powder is sieved to a mesh size of 50, weighed, and added to water at a material-to-liquid mass ratio of 1:3. The mixture is stirred thoroughly until a cassava powder aqueous solution is obtained. Add α-amylase at a rate of 10 U / g based on the weight of the cassava flour to the cassava flour solution. Incubate the mixture at 50-60°C, maintaining the pH between 6.0-7.0, allowing the α-amylase to act on the cassava flour and break down starch molecules. After 2 hours of α-amylase action, when an increase in solution viscosity is observed, add saccharifying enzyme at a rate of 200 U / g based on the weight of the cassava flour. Continue to maintain the temperature at 60°C and the pH at approximately 5.0-6.0, allowing the saccharifying enzyme to convert the remaining amylose into glucose. After 1 hour of saccharification, add protease at a rate of 0.01%-0.05% based on the weight of the cassava flour. Enzymatically hydrolyze the solution in a constant temperature water bath at 45°C for 120 minutes, maintaining the pH at approximately 5.0-7.0, to degrade the protein components in the solution, yielding enzymatically hydrolyzed cassava flour.

[0078] Specifically, the preparation method of enzymatically hydrolyzed bamboo powder and peanut shell powder is as follows: Peanut shell powder is sieved through a 200-mesh sieve, and bamboo powder is sieved through a 300-mesh sieve. The peanut shell powder and bamboo powder are weighed and pretreated by soaking in a 0.1 mol / L dilute sulfuric acid solution for 10 minutes to remove some lignin and soften the fiber structure, thereby improving enzymatic hydrolysis efficiency. Water is added at a material-to-liquid mass ratio of 1:10, and the mixture is stirred thoroughly until a mixed aqueous solution is obtained. Xylanase is added to the mixture solution at a concentration of 5 U / g based on the mass of the mixture. The mixture is kept at 40-50°C, maintaining the pH between 4.5-5.5, allowing the xylanase to act on the hemicellulose in the bamboo powder and peanut shells. After the xylanase has acted for 1 hour, β-glucosidase is added at a concentration of 20 U / g based on the mass of the mixture. The temperature is maintained at 45-60°C and the pH at approximately 5.0-6.0, allowing the β-glucosidase to decompose the resulting oligosaccharides. After 30 minutes of β-glucosidase action, manganese peroxidase is added at 0.01%-0.05% of the mixture mass. The mixture is then enzymatically hydrolyzed in a constant temperature water bath at 50°C for 60 minutes, with the pH value around 5.0-6.0, to obtain enzymatically hydrolyzed bamboo powder and peanut shell powder.

[0079] Specifically, during the aquaculture process, it is necessary to regularly monitor the dynamic changes in pond water quality indicators, biofloc quantity, bacterial count, and algal growth. This involves checking whether the five indicators of suspended solids, pH, chemical oxygen demand, total nitrogen, and total phosphorus in the aquaculture pond water meet the freshwater aquaculture wastewater discharge limits, and examining the changes in important water quality indicators affecting shrimp growth, such as dissolved oxygen, ammonia nitrogen, nitrite, total alkalinity, and total hardness. Changes in the amount of biofloc in the pond water are measured, the number of heterotrophic bacteria and other microorganisms is detected, and the dominant species and abundance of microalgae in the mid-to-late stages are determined. Aquaculture production performance, such as the culture cycle, survival rate, unit yield, and feed conversion ratio, is analyzed. When C / N < 10, the water purification capacity is low, and Vibrio bacteria easily grow and reproduce, which is extremely detrimental to shrimp farming; when C / N > 15, bioflocs can effectively purify the water and effectively reduce the occurrence of Vibrio disease.

[0080] like Figure 1 , Figures 3 to 19 As shown, the implementation method of Example 1 is as follows: An application of an enzymatically hydrolyzed composite carbon source in shrimp farming includes a pond 1. The pond 1 includes a liquid oxygen supply assembly 2, an aeration pump assembly 3, and a feed dispensing device 4. The liquid oxygen supply assembly 2 includes a liquid oxygen input device 5 located on one side of the pond 1, a liquid oxygen output device 6 located on the pond surface, and a transmission pipe 23 connecting the liquid oxygen input device 5 and the liquid oxygen output device 6. The aeration pump assembly 3 is located on the pond surface and works in conjunction with the liquid oxygen output device 6 to supply oxygen. The feed dispensing device 4 is used to add feed to the pond surface after oxygen supply is completed. This invention, through the cooperation of the aeration pump assembly 3 and the liquid oxygen supply assembly 2, can stably provide oxygen under any climatic conditions, unaffected by weather. The liquid oxygen supply method can provide a higher concentration of oxygen, thereby increasing the feeding frequency of Litopenaeus vannamei. Combined with the feed dispensing device 4, the shrimp are fed, improving farming efficiency.

[0081] This invention has the advantages of simple structure, convenient operation, good oxygenation effect, energy saving and environmental protection, and is suitable for shrimp farms of various sizes. The liquid oxygen input device 5 has two units arranged diagonally, including a vaporization component connected to the liquid oxygen cylinder, a flow meter, and a pressure regulating device. The liquid oxygen output device 6 includes a float plate 61 floating on the water surface, a liquid oxygen output shell 62 connected to the float plate 61 and partially extending into the water, a delivery pump 63 located on the liquid oxygen output shell 62 and connected to the transmission pipe 23, and an aeration component 64 connected to the delivery pump 63. The aeration component 64 is connected to the delivery pump 63 through an aeration component air pipe 641. The liquid oxygen output housing 62 includes a filter assembly 621 connected to the outside of the aeration element 64, and a rotating assembly 622 rotatable relative to the filter assembly 621. The rotating assembly 622 has a support frame 6221, a motor 6222 located on the support frame 6221, a rotating shaft 6223 connected to the motor 6222, and blades 6225 connected to the rotating shaft 6223. The filter assembly 621 has through holes 6211 that are submerged in water. The through holes 6211 are arranged in multiple regularly spaced positions.

[0082] The filter assembly 621 is provided with an anti-clogging component 6212. The anti-clogging component 6212 includes a cleaning rod 62121 through which the rotating shaft 6223 passes and is connected to the rotating shaft 6223. The cleaning rod 62121 has a cleaning rod groove 621211 slidably connected to the cleaning rod groove 621211, a cleaning block 621212, and an elastic reset member 621213 located between the inner wall of the cleaning rod groove 621211 and the cleaning block 621212. The cleaning block 621212 can extend into the through hole 6211. The cleaning block 621212 is made of an elastic material. The filter assembly 621 is made of an elastic material.

[0083] The filter assembly 621 includes a filter cylinder 62153, and a filter cylinder fixing sleeve 62156 is fixedly connected to the outer wall of the filter cylinder 62153. A first slider 62157 is slidably connected to the filter cylinder fixing sleeve 62156. The outer wall of the first slider 62157 is fixedly connected between a rotating rod 62155 and a blade 6225. The rotating rod 62155 has a slot 621551. The filter cylinder 62153 has a filter cylinder groove 621531 for the first slider 62157 to slide. The filter cylinder fixing sleeve 62156 is circular, allowing the first slider 62157 to rotate in a circular motion within the filter cylinder groove 621531, thus driving the blade 6225 to rotate.

[0084] A rotating shaft 6223 is slidably connected to a moving rod 621521. One end of a spring 621524 is fixedly connected to the outer wall of the moving rod 621521, and the other end of the spring 621524 is fixedly connected to the inner wall of the rotating shaft 6223. A locking rod 621525 is fixedly connected to the end of the moving rod 621521. A first sliding sleeve 621522 is fixedly connected to the outer wall of the moving rod 621521, and is slidably connected to the rotating shaft 6223. A threaded sleeve 621523 is rotatably connected to the first sliding sleeve 621522. Two sets of liquid oxygen output housings are provided, and a buffer distance is provided between the aeration element 64 and the liquid surface. The feed spreading device 4 includes a feed spreading shell 41 and a base 42 connected to the bottom of the feed spreading shell 41. The uppermost layer of the base 42 is provided with a feeding component 421, a cutting component 422 located below the feeding component 421, and a blowing component 423 connected to the cutting component 422. The feed spreading shell 41 has a feed spreading shell opening 44 on its outer side. The blowing component 423 is used to blow the feed cut by the cutting component 422 to the outside of the feed spreading shell opening 44.

[0085] A filter plate 424 is provided between the cutting assembly 422 and the blowing assembly 423. A rotatable striking assembly 425 is connected to the lower side of the filter plate 424 and contacts the filter plate 424. A conveying inclined surface 426 is provided on the lower side of the filter plate 424. A conveying area 427 is provided at the bottom of the conveying inclined surface 426. The conveying area 427 is located between the blowing assembly 423 and the feed spreading shell opening 44.

[0086] The feed spreading shell 41 contains a crushing bin 413, a cutting assembly 422 is located inside the crushing bin 413, a fixing plate 418, and a first motor 417 is fixedly connected below the fixing plate 418. The cutting assembly 422 is located below the first motor 417. The cutting assembly 422 includes a first rotating wheel 42291, which is fixedly connected to the output shaft of the first motor 417. A round rod 422917 is fixedly connected below the first rotating wheel 42291. Crushing rods 422918 are fixedly connected around the outer wall of the round rod 422917. The striking assembly 425 includes the following... The structure is as follows: A conveyor belt 42292 is drivenly connected to the first rotating wheel 42291. A second rotating wheel 42293 is drivenly connected to the end of the conveyor belt 42292 away from the first rotating wheel 42291. A rotating shaft 42294 is fixedly connected to the second rotating wheel 42293. A fixing plate 42295 is rotatably connected to the rotating shaft 42294, and the fixing plate 42295 is fixedly connected to the outer wall of the feed spreading shell 41. A second fixing plate 42296 is fixedly connected below the fixing plate 42295. A bevel gear is fixedly connected below the rotating shaft 42294. The second bevel gear 422910 meshes with a first bevel gear 42299. The first bevel gear 42299 is fixedly connected to a second rotating shaft 42298, and the second rotating shaft 42298 is rotatably connected to a second fixed plate 42296. Further, a rotating block 422911 is fixedly connected to the end of the second rotating shaft 42298 away from the second fixed plate 42296. The rotating block 422911 is fixedly connected to a cylinder 422921. The cylinder 422921 is slidably connected to a connecting rod 422912. The connecting rod 422912 is fixedly connected to a sector block. 422915, the sector block 422915 is rotatably connected to a fixing plate three 422916, the fixing plate three 422916 is fixedly connected to the lower part of the fixing plate one 42295, multiple sets of toothed blocks are fixedly connected to the lower part of the sector block 422915, the multiple sets of toothed blocks mesh with a gear 422913, the gear 422913 is fixedly connected to a rotating rod 422914, the rotating rod 422914 penetrates the inner wall of the feed spreading shell 41, and a striking component 425, such as a hammer 4250, is fixedly connected to the outer wall of the end of the rotating rod 422914 away from the gear 422913.

[0087] The filter plate 424 has mesh openings. The conveying area 427 includes a tilting channel 4271 connected to the outside of the crushing hopper 413, and a blowing port 4272 connected to the feed spreading shell opening 44. The tilting channel 4271 and the blowing port 4272 are connected. The blowing port 4272 is inclined from bottom to top. The blowing assembly 423 includes a blower, which is fixed to the lower side of the base plate 42. The feed spreading shell 41 has a cover on its upper side to prevent moisture from entering the feeding assembly and affecting the dryness of the feed.

[0088] The feeding assembly 421 includes a feeding hopper 4201 that is larger at the top and smaller at the bottom, a movable feeding plate 4202 located below the feeding hopper 4201, a feeding trough 4207 located below the feeding plate 4202, a feeding limiting rod 4203 connected to the feeding plate 4202 and fixed inside the feed spreading shell 41, and a feeding motor 4204 connected to the feeding plate 4202 and fixed at one end inside the feed spreading shell 41. A feeding movable rod 4205 is connected between the feeding motor 4204 and the feeding plate 4202. The feeding plate 4202 is provided with a relief groove 4206 for limiting the feeding limiting rod 4203 to move forward and backward. The feeding motor 4204 is a lead screw motor.

[0089] The oxygenation pump assembly 3 has two units, which are diagonally arranged on the side away from the liquid oxygen supply assembly 2. This reduces the impact on the biofloc around the liquid oxygen supply assembly 2 when it is turned on. It includes a support 31 fixed at one end to the pond, a booster pump located on the support 31, multiple buoyancy components 32 symmetrically arranged on the outside of the support 31, and an anti-tipping assembly 33 connecting the support 31 and the buoyancy components 32. The anti-tipping assembly 33 includes a sliding component 331 for the buoyancy components 32 to move up and down, and a hinge component 332 connecting the sliding component 331 and the buoyancy components 32. When the buoyancy components 32 move upward along the sliding component 331, the hinge component 332 drives the buoyancy components 32 to move closer to the center of the support 31.

[0090] The support member 31 includes an upper panel 311 fixed at one end to the pond and a fixed platform 334 connected to the hinge assembly 332 for placing a booster pump. A support rod 312 is fixedly connected to the bottom surface of the upper panel 311. A sliding groove 3121 is formed on the surface of the support rod 312. A sliding sleeve 313 is provided between the inner wall of the sliding groove 3121 and the surface of the support rod 312. The hinge assembly 332 includes a long arc rod 3321 and a short arc rod 3322. The surface of the sliding sleeve 313 is hinged to one end of the long arc rod 3321 by a coil spring. The other end of the long arc rod 3321 is hinged to one end of the short arc rod 3322. The other end of the short arc rod 3322 is hinged to the surface of the fixed platform 334. A fixing ring 33211 is fixedly connected to the surface of the long arc rod 3321. The buoyancy assembly 32 is a float fixedly connected inside the fixing ring 33211.

[0091] Additionally, the fixed platform 334 includes a platform shell 334201, a rotating rod 334203, and a platform fixing plate 334205. A limit block 334202 is fixedly connected to the upper surface of the platform shell 334201. The interior of the platform shell 334201 is rotatably connected to the upper surface of the rotating rod 334203. One end of a spring 334204 is fixedly connected to the surface of the platform fixing plate 334205, and the surface of the platform fixing plate 334205 is fixedly connected to the interior of the platform shell 334201. A fixing buckle 334206 and a toggle lever 334207 are fixedly connected to the upper surface of the rotating rod 334203.

[0092] Example 2: As Figure 2 As shown, the difference between Embodiment 2 and Embodiment 1 is that the base 42 is further connected to a first float 4200, a second float 4208, and a drive assembly, which supports the entire feed spreading shell 41 and drives the feed spreading device to move on the water surface. The drive assembly includes a drive motor and a stirring paddle 420. The blowing assembly 423 includes four blowers located at the four corners of the conveying area 427. The conveying ramp 426 is pyramidal in shape and includes four ramps corresponding one-to-one with the conveying area 427. The feed spreading shell opening 44 is also provided with four openings, so that feed can be spread simultaneously from all four sides of the feed spreading shell 41, improving the spreading efficiency.

[0093] The feed distributing device also includes a control unit and a photovoltaic cell module connected to the control unit. The feed distributing device can be charged by the photovoltaic cell module under sunlight, improving its ease of use. Additionally, the control unit includes a remote control component, allowing users to connect via wireless network, Bluetooth, infrared, etc., and remotely control the movement of the feed distributing device using tools such as computers, mobile phones, or remote controls. Furthermore, the control unit can adjust the feed size according to the preset growth cycle of the Pacific white shrimp. Specifically, when the Pacific white shrimp are in the larval stage, the control unit increases the rotation speed of the cutting component 422, further reducing the feed size. When the Pacific white shrimp are in the adult stage, the control unit slows down or even stops the rotation of the cutting component 422, ensuring the feed size is suitable for the Pacific white shrimp's consumption, preventing excessively fine feed from being ingested by adult shrimp and dissolving directly in the pond, causing environmental pollution and waste.

[0094] Example 3: Based on Example 1, Example 3 further includes the following implementation method: a method for applying enzymatic hydrolysis of a complex carbon source in shrimp farming, comprising the following steps: S1. A liquid oxygen input device 5 is installed on one side of the pond. The flow rate of liquid oxygen to be transported in a single operation is preset, and the start time of the oxygenation pump assembly 3 is preset. The liquid oxygen input device 5, the oxygenation pump assembly 3, and the liquid oxygen output device 6 are started simultaneously for 1 hour. After the S2, liquid oxygen input device 5, oxygenation pump assembly 3, and liquid oxygen output device 6 have completed their preset operation time, the feed spreading device 4 divides the feed and spreads it into the pond at a preset speed. The feed spreading device 4 adopts a continuous feeding method for 12 hours from 7:00 am to 7:00 pm, and starts spreading for 2 minutes every 10 minutes. S3, the liquid oxygen input device 5, the aeration pump assembly 3, and the liquid oxygen output device 6 in steps S1 and S2 are operated at least 5 times a day. The liquid oxygen input device, by controlling the amount of liquid oxygen input in conjunction with the liquid oxygen output device, adds 0.1 grams of oxygen to each cubic meter of water in the pond every day.

[0095] Before step S1, there is step S100: During the first aquaculture, seawater is added to the aquaculture pond, and the salinity is adjusted to about 0.5% to 2.5% with fresh water. The water temperature is controlled at 20-30℃, and then 20-30g / m³ of bleaching powder is added. 3 Disinfect the water with 100 kg / mu of quicklime. After aeration for 1-2 days until there is no residual chlorine in the water, add EM bacteria and amino acid fertilizer paste seven days before stocking. Utilize the colony-forming organisms to occupy space. Every morning, apply Nitrosomonas, Denitrifying Pseudomonas, Bacillus subtilis, photosynthetic bacteria, and Lactobacillus acidophilus to the entire pond, with an addition amount of approximately 7.5 × 10⁻⁶ for Nitrosomonas. 6 cfu·L -1 Denitrifying Pseudomonas aeruginosa 6.0 × 10 6 cfu·L -1、 Bacillus subtilis 7.0 × 10 6 cfu·L -1 Rhodospirillumaceae 3.0×10 8 cfu·L -1 Lactobacillus acidophilus 1.0 × 10 6 cfu·L -1 Simultaneously, add 1 kg of brown sugar per mu (approximately 0.067 hectares) and sprinkle it throughout the pond. After continuous application for 5-7 days, flocculent particles will appear in the water, and bioflocs will begin to form. The stocking density of shrimp larvae should be 100-200 shrimp / m². 3 The introduction of microalgae brought the algae concentration in the water to 5 × 10⁻⁶. 4 CFU / mL. The microalgae used are Chlorella vulgaris, Scenedesmus stenoptera, Arachnium platensis, and Arachnium macrocarpa. Continuous aeration for 15 days was performed to achieve a biofloc concentration of over 5 ml / L (sedimentation volume in half an hour) and maintain stability.

[0096] Step S3 further includes the following step, S301: Before each addition of the carbon source, the soaking solution contains 2-5 mL per liter at a concentration of 9.5 × 10⁻⁵ mL.6 cfu·L -1 The lactic acid bacteria solution improved the intestinal absorption of shrimp larvae within 10 minutes. For the first 30 days after stocking, supplement with organic carbon at 50% of the daily feed intake, and simultaneously introduce Nitrosomonas, Denitrifying Pseudomonas, Bacillus subtilis, Rhodospirillum, and Lactobacillus acidophilus every 5 days, at a dosage of approximately 7.5 × 10⁻⁶ for Nitrosomonas. 6 cfu·L -1 Denitrifying Pseudomonas aeruginosa 6.0 × 10 6 cfu·L -1、 Bacillus subtilis 7.0 × 10 6 cfu·L -1 Rhodospirillumaceae 3.0×10 8 cfu·L -1 Lactobacillus acidophilus 1.0 × 10 6 cfu·L -1 In the later stages of aquaculture, 30% of the feed should be supplemented with organic carbon materials.

[0097] During the aquaculture process, potassium persulfate is used every 10-20 days to improve the bottom sediment. Sodium carbonate solution and quicklime are added to adjust the water pH to between 7.1 and 8.1 and the total alkalinity to between 150 mg and 300 mg / L. In the later stages of aquaculture, the concentration of bioflocs in the water is controlled to remain stable at 15-30 mL / L. No water is changed throughout the entire aquaculture process; only a small amount of fresh water is used to replenish the water lost due to evaporation.

[0098] In the later stages of cultivation, apply 500g / mu of Bacillus subtilis solution and 800g / mu of Lactobacillus acidophilus solution daily. Every 3-5 days, apply 10kg / mu of EM (Effective Microorganisms) solution to improve algae stability. Turn on the aerator within one hour after application. Maintain a C / N ratio between 15-20. The mixing ratio of bamboo powder, peanut shell powder, brown sugar, and cassava powder is 10 parts bamboo powder, 20 parts peanut shell powder, 30 parts brown sugar, and 40 parts cassava powder.

[0099] The preparation method of enzymatically hydrolyzed cassava flour is as follows: Sift the cassava flour through a 50-mesh sieve, weigh out the cassava flour, add it to water at a material-to-liquid mass ratio of 1:3, and stir thoroughly until a cassava flour aqueous solution is obtained. Add α-amylase to the cassava flour solution at a rate of 10 U / g based on the mass of the cassava flour. Keep the mixture at 50-60°C and maintain the pH between 6.0 and 7.0 to allow the α-amylase to act on the cassava flour and decompose starch molecules. After 2 hours of α-amylase action, when an increase in solution viscosity is observed, add saccharifying enzyme at a rate of 200 U / g based on the mass of the cassava flour. Continue to maintain the temperature at 60°C and the pH at approximately 5.0-6.0 to allow the saccharifying enzyme to convert the remaining amylose into glucose. One hour after saccharification, 0.01%-0.05% of protease by weight of cassava flour is added, and the mixture is enzymatically hydrolyzed in a constant temperature water bath at 45°C for 120 minutes at a pH of approximately 5.0-7.0 to degrade the protein components in the solution and obtain enzymatically hydrolyzed cassava flour.

[0100] The preparation method of enzymatically hydrolyzed bamboo powder and peanut shell powder is as follows: Peanut shell powder is sieved through a 200-mesh sieve, and bamboo powder is sieved through a 300-mesh sieve. The peanut shell powder and bamboo powder are weighed and pretreated by soaking in a 0.1 mol / L dilute sulfuric acid solution for 10 minutes to remove some lignin and soften the fiber structure, thereby improving enzymatic hydrolysis efficiency. Water is added at a material-to-liquid mass ratio of 1:10, and the mixture is stirred thoroughly until a mixed aqueous solution is obtained. Xylanase is added to the mixture solution at a concentration of 5 U / g based on the mass of the mixture. The mixture is kept at 40-50°C, maintaining the pH between 4.5-5.5, allowing the xylanase to act on the hemicellulose in the bamboo powder and peanut shells. After the xylanase has acted for 1 hour, β-glucosidase is added at a concentration of 20 U / g based on the mass of the mixture. The temperature is maintained at 45-60°C and the pH at approximately 5.0-6.0, allowing the β-glucosidase to decompose the oligosaccharides produced. After 30 minutes of β-glucosidase action, manganese peroxidase is added at 0.01%-0.05% of the mixture mass. The mixture is then enzymatically hydrolyzed in a constant temperature water bath at 50°C for 60 minutes, with the pH value around 5.0-6.0, to obtain enzymatically hydrolyzed bamboo powder and peanut shell powder.

[0101] Comparative Example 1: The difference between Comparative Example 1 and Example 3 is that the feeding management of Comparative Example 1 is as follows: During the initial breeding phase, feed the animals three times a day at fixed times. Every three days, add a mixture of photosynthetic bacteria, yeast, fermented flour, and rice bran to the breeding pond at a rate of 5 catties per acre. This mixture replaces the original organic carbon sources such as bamboo powder, peanut shell powder, brown sugar, cassava powder, Nitrifying Monotrophus, Denitrifying Pseudomonas, Bacillus subtilis, Rhodospirillum, and Lactobacillus acidophilus. The mixture is prepared by adding 50 kg of carbon source to 1000 liters of water, followed by 5 liters of a mixed solution of photosynthetic bacteria and yeast colonies at a concentration of 1 billion CFU / mL. Ferment this mixture in a sealed container at a temperature above 22 degrees Celsius for 24 hours before use. The carbon source is fermented flour and rice bran. Add the mixture every 10 days... Add potassium bisulfate and quicklime to the aquaculture pond to adjust the pH value. For the first 5 days, feed regular feed at a rate of 0.1 kg per 10,000 seedlings, three times a day. Starting from the sixth day, increase the feed amount every two days, specifically by 0.5 kg per meal, continuing until the 20th day. Observe using the feeding trough and adjust the feed amount according to the feeding situation. After one month of aquaculture, the water body will reach a stable period. During the water body stabilization period, feed five times a day at fixed times. During the feeding management process, stir the bottom mud every other day, and add a mixture of photosynthetic bacteria, yeast, fermented flour, and rice bran to the aquaculture pond at a rate of 3 catties per acre every day. Add potassium bisulfate and quicklime to the aquaculture pond every 5 days to adjust the pH value.

[0102] Comparative Example 2: The difference between Comparative Example 2 and Example 3 is that Comparative Example 2 uses a common mixing ratio of bamboo powder, peanut shell powder, brown sugar and tapioca powder, with bamboo powder accounting for 10 parts, peanut shell powder accounting for 20 parts, brown sugar accounting for 30 parts and tapioca powder accounting for 40 parts.

[0103] Comparative Example 3: The difference between Comparative Example 3 and Example 3 is that Comparative Example 3 uses photosynthetic bacteria and yeast instead of the original Nitrosomonas, Denitrifying Pseudomonas, Bacillus subtilis, Rhodospirillumaceae and Lactobacillus acidophilus in its feeding management.

[0104] Comparative Example 4: The difference between Comparative Example 4 and Example 3 is that Comparative Example 4 does not use a liquid oxygen supply component.

[0105] Table 1: Aquaculture results of the present invention in Litopenaeus vannamei

[0106] As shown in Table 1, compared with Example 1, Comparative Example 1 reduced the frequency of use of bacterial strains and organic carbon sources. The replacement of bacterial strains reduced the photosynthesis of algae. The reduction of Lactobacillus acidophilus and Bacillus subtilis will correspondingly increase the number of Vibrio and affect the survival rate of Litopenaeus vannamei. The replacement of organic carbon sources will correspondingly reduce the formation of bioflocs and affect the absorption and digestion of Litopenaeus vannamei. Fixed feeding time also caused excess organic matter and feed to settle in the water, resulting in an imbalance of carbon-nitrogen ratio.

[0107] Compared with Example 1, under the same feeding interval, the treated organic carbon source in Example 1 was more easily ingested and absorbed by microorganisms or indirectly by Litopenaeus vannamei. Example 1 reduced the amount of residual organic matter in the water and maintained a stable carbon-to-nitrogen ratio. The survival rate of Litopenaeus vannamei in Example 1 was higher. Compared with Comparative Example 1, Comparative Example 2 was fed in small amounts at intervals, which could minimize the precipitation of excessive organic matter. However, the unmodified organic carbon source could not be effectively absorbed and utilized by microorganisms to form flocs, which would affect the feeding and absorption of Litopenaeus vannamei to some extent. The multiple bacterial species in Comparative Example 2 could reduce the number of Vibrio bacteria, which could relatively improve the survival rate of Litopenaeus vannamei.

[0108] Compared with Example 1, the strains in Example 1 can reduce the ammonia nitrogen content in the water, thereby helping to improve water quality. In Example 1, the number of Vibrio bacteria was reduced and the survival rate of Litopenaeus vannamei was improved by Lactobacillus acidophilus and Bacillus subtilis. Compared with Comparative Example 1, Comparative Example 3 was fed in small amounts at intervals. The treated organic carbon source was more easily ingested and absorbed by microorganisms or Litopenaeus vannamei. Comparative Example 3 correspondingly reduced the amount of residual organic matter in the water, which can relatively improve the survival rate and size of Litopenaeus vannamei.

[0109] Comparing Comparative Example 4 and Example 1, it can be seen that in Comparative Example 4, due to the reduction of the liquid oxygen supply component, the amount of oxygen in the water is correspondingly reduced. In Example 1, the Pacific white shrimp located near the liquid oxygen output device have a high oxygen concentration that can be ingested and a higher appetite, so the size of the Pacific white shrimp will be relatively larger. The liquid oxygen output device can replenish the oxygen consumed by microorganisms in the water, thereby ensuring the survival rate of the Pacific white shrimp.

Claims

1. Application of enzymatic complex carbon source in prawn culture, comprising a pond, characterized in that, The pond is provided with a liquid oxygen supply assembly, an oxygenation pump assembly, and a feed spreading device, the liquid oxygen supply assembly includes a liquid oxygen input device arranged at one side of the pond, a liquid oxygen output device arranged at the liquid surface of the pond, and includes the following steps: S100, sea water is added to the pond, the salinity is adjusted with fresh water, the water body is disinfected, and the biological flocculation is formed by using the bacterial colony biological occupation, and the shrimp seedlings and microalgae are put in; S1, the liquid oxygen input device installed at one side of the pond, preset the flow of liquid oxygen needed to be transported at one time, preset the starting time of the oxygenation pump assembly, the liquid oxygen input device, the oxygenation pump assembly and the liquid oxygen output device are started at the same time; S2, after the liquid oxygen input device, the oxygenation pump assembly and the liquid oxygen output device run for a preset time, the feed spreading device divides and sprays the feed to the pond at a preset speed; S3, steps S1 and S2 are repeated at least 3-5 times a day, and the organic carbon source subjected to enzymolysis is put in.

2. The use of the enzymatic complex carbon source according to claim 1 in prawn culture, characterized in that, In step S1, the liquid oxygen input device controls the input amount of liquid oxygen in cooperation with the liquid oxygen output device to add 0.05-0.1 grams of oxygen per cubic meter of water body in the pond per day.

3. The use of the enzymatic complex carbon source according to claim 1 in prawn culture, characterized in that, In step S2, the feed spreading device adopts a 12-hour uninterrupted feeding method, and is started for 2 minutes every 10 minutes.

4. The use of the enzymatic complex carbon source according to claim 1 in prawn culture, characterized in that, The oxygenation pump assembly is provided with two and is diagonally arranged on the side away from the liquid oxygen supply assembly.

5. The use of the enzymatic complex carbon source according to claim 1 in prawn culture, characterized in that, The feed spreading device is provided with a control member and a photovoltaic cell assembly connected with the control member, the control member includes a remote control assembly for remotely controlling the movement of the feed spreading device, and the control member adjusts the size of the feed according to the preset growth cycle of the penaeus vannamei.

6. The use of the enzymatic complex carbon source according to claim 1 in prawn culture, characterized in that, In step S1, the bacterial colonies include nitrosomonas, pseudomonas denitrificans, bacillus subtilis, photosynthetic bacteria and lactobacillus acidophilus, and the microalgae include chlorella, scenedesmus, arthrospira platensis and arthrospira maxima.

7. Use of the enzymatic complex carbon source according to claim 1 in prawn farming, characterized in that, In step S3, the organic carbon source is soaked in 2-5 mL / L lactobacillus solution for 10 minutes before being added, and the organic carbon source is supplemented and added at 50% of the feed feeding amount every day within 30 days after the seedlings are put in, and the bacterial colonies are added every 5 days.

8. Use of the enzymatic complex carbon source according to claim 1 in prawn farming, characterized in that, In step S3, 30% of the feeding amount is added to the organic carbon source in the middle and later stages of breeding, and bacillus subtilis solution and lactobacillus acidophilus solution are sprayed every day, and EM bacteria solution is sprayed every 3-5 days.

9. Use of the enzymatic complex carbon source according to claim 1 in prawn farming, characterized in that, In step S3, the organic carbon source includes bamboo powder, peanut shell powder, brown sugar and cassava powder, and the bamboo powder accounts for 10-20 parts, the peanut shell powder accounts for 15-25 parts, the brown sugar accounts for 30-50 parts, and the cassava powder accounts for 30-40 parts.

10. The use of the enzymatic complex carbon source according to claim 9 in prawn culture, characterized in that, In step S3, the cassava powder is subjected to enzymolysis by alpha-amylase, glucoamylase and protease, and the bamboo powder and peanut shell powder are subjected to enzymolysis by xylanase, beta-glucosidase and manganese peroxidase.

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