Process and device for producing small-molecular-weight humanized III-type collagen

Abstract

The invention discloses a small-molecular-weight humanized III-type collagen production process and device, and relates to the technical field of biological product production, and the device comprises a raw material feeding ring, a raw material crushing assembly, a material transition assembly, a centrifugal separation assembly and a product discharge port which are fixedly connected in sequence; the centrifugal separation assembly and the raw material smashing assembly are in power linkage and achieve synchronous operation with the help of the same rotating power. According to the device, raw material preparation is completed through a gene editing device: a gene cleavage enzyme of a specific fragment is designed, the cleavage enzyme is used for cleavage of a human gene to obtain a specific gene fragment, gene fragments corresponding to 458 amino acids in SEQIDNO: 1 are intercepted for expression, a target precursor raw material with the diameter being 1.4 nm and the length being 280 nm is obtained, the transdermal performance of molecules with the diameter being 1.4 nm is higher, and the specific gene fragment is obtained. The sweat gland penetrates through the basal layer to directly reach the corium layer, and the molecules can be rapidly distributed on the wound surface and cover the wound surface without dead corners to promote granulation growth and crawling to accelerate healing.

Description

Technical Field

[0001] This invention relates to the field of biopharmaceutical technology, specifically to a process and apparatus for producing low molecular weight humanized type III collagen. Background Technology

[0002] Low molecular weight humanized type III collagen has wide applications in medical aesthetics and biomedical materials due to its excellent biocompatibility, biodegradability, and cell affinity. Raw material grinding and centrifugation are key processes in its production, directly affecting product purity and yield. Current production equipment generally suffers from the following problems: First, raw material grinding is mostly single-stage grinding, making it difficult to flexibly adjust grinding parameters according to the raw material's state, easily leading to insufficient grinding and uneven particle size, making subsequent separation difficult; second, grinding and centrifugation mechanisms often use independent power systems, resulting in complex structures, high manufacturing costs, and significant energy consumption; third, material accumulation and sudden flow rate changes during inter-process transfer can cause residue or splashing problems, affecting production continuity and efficiency; fourth, the equipment has poor adaptability, making it difficult to accommodate raw materials in different initial states, resulting in insufficient versatility. Therefore, developing a production device that can achieve precise graded grinding, efficient power linkage, smooth material transfer, and strong versatility has become an urgent technical need to be addressed in the current production of low molecular weight humanized type III collagen. Summary of the Invention

[0003] The purpose of this invention is to provide a process and apparatus for producing low molecular weight humanized type III collagen, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] A process and apparatus for producing low molecular weight humanized type III collagen includes a raw material feeding ring with a circular shell through which raw materials can pass. A raw material crushing component is fixedly connected below the raw material feeding ring. A material transition component is fixedly connected below the raw material crushing component. A centrifugal separation component is fixedly connected below the material transition component. A product outlet is fixedly connected below the centrifugal separation component. The raw material crushing component is responsible for thoroughly crushing the raw materials. The raw material crushing component includes a crushing chamber shell and a built-in crushing mechanism. The crushing chamber shell is a cylindrical shell. The internal structure includes a built-in crushing mechanism. The material transition component includes a transition guide block and a variable diameter transition cavity. The transition guide block is a cylindrical block with a hole at its center. The variable diameter transition cavity is composed of a symmetrical frustum-shaped shell and a cylindrical shell fixedly connected to each other. The centrifugal separation component is responsible for centrifuging the processing liquid flowing out of the material transition component. The centrifugal separation component includes a centrifugal chamber shell and a built-in centrifugal mechanism. The centrifugal chamber shell is a cylindrical shell, and the built-in centrifugal mechanism is disposed inside the centrifugal chamber shell. The bottom of the centrifugal chamber shell is fixedly connected to the product outlet.

[0006] As a preferred embodiment of the present invention, the built-in crushing mechanism includes a drive turntable, the drive turntable having a built-in rotary motor, a crushing adjustment seat fixedly connected to the bottom of the drive turntable, the crushing adjustment seat having a built-in drive motor, three upper adjustment rods and a lower adjustment rod arranged around the crushing adjustment seat, the three upper adjustment rods and the lower adjustment rods being arranged in a ring, the upper adjustment rods and the lower adjustment rods being on the same vertical line, the lower adjustment rod being located below the upper adjustment rods, the tail ends of the upper adjustment rods and the lower adjustment rods being fixedly connected to a grading crushing blade assembly, and a preliminary crushing blade being fixedly connected to the bottom end of the crushing adjustment seat.

[0007] As a preferred embodiment of the present invention, the grading and crushing blade assembly includes a blade assembly mounting plate, which is a rectangular block. An upper adjusting slider, a lower adjusting slider, and a refining and crushing blade are sequentially fixedly connected to the mounting plate from top to bottom. The upper adjusting slider is movably sleeved within the upper adjusting rod, allowing for lateral movement within the rod. The lower adjusting slider is movably sleeved within the lower adjusting rod, allowing for lateral movement within the rod.

[0008] As a preferred embodiment of the present invention, the feature is that: three fine pulverizing blades are provided, and the three fine pulverizing blades are evenly distributed.

[0009] As a preferred technical solution of the present invention, the built-in centrifugal mechanism includes a centrifugal rotating frame, which is a ring with four fan-shaped holes. A circular hole is opened at the center of the centrifugal rotating frame. The centrifugal rotating frame is fixedly connected to the preliminary crushing blade. Centrifugal separation chambers are fixedly connected to the fan-shaped holes on the centrifugal rotating frame. The centrifugal separation chamber is a fan-shaped shell. A product guide groove is fixedly connected to the bottom of the centrifugal separation chamber.

[0010] As a preferred embodiment of the present invention, the drive turntable is frustum-shaped and the outer wall of the drive turntable is smooth.

[0011] Compared with the prior art, the beneficial effects of the present invention are: (1) A process and apparatus for producing low molecular weight humanized type III collagen, which realizes precise crushing of raw materials and improves the raw material processing effect: The present invention achieves preliminary crushing of raw materials by driving a turntable to drive a preliminary crushing blade, and then uses a crushing adjustment seat to drive a horizontally adjustable fine crushing blade to flexibly adapt to different raw material states and adjust the penetration depth to form a graded crushing structure, making the raw material crushing more uniform and sufficient, effectively reducing the difference in raw material particle size, and laying a high-quality material foundation for the subsequent centrifugal separation process.

[0012] (2) A process and apparatus for producing low molecular weight humanized type III collagen, with a power linkage design that simplifies the structure and reduces the cost and energy consumption of the apparatus: by fixing the centrifugal rotating frame with the built-in centrifugal mechanism to the preliminary crushing blade, the centrifugal separation component is driven synchronously by the rotational power of the drive turntable, without the need for additional centrifugal power components. This not only simplifies the overall structure of the apparatus and reduces the number of parts, but also reduces the manufacturing cost and operating energy consumption of the equipment, thereby improving the economic efficiency of the apparatus.

[0013] (3) A process and apparatus for producing low molecular weight humanized type III collagen, which optimizes the material flow structure and ensures smooth and efficient processing: a frustum-shaped drive turntable with smooth outer wall is adopted to guide the raw materials to disperse and slide around to avoid accumulation; the material transition component composed of transition guide block and variable diameter transition cavity makes the flow rate of the processing liquid transition smoothly, effectively avoiding problems such as raw material residue and processing liquid splash caused by sudden change in flow rate, ensuring smooth transmission of materials between processes and improving overall production efficiency.

[0014] (4) A process and apparatus for producing low molecular weight humanized type III collagen, wherein the adjustable crushing structure enhances the versatility of the apparatus: by the movable connection between the upper adjusting slider and the upper adjusting rod, and the lower adjusting slider and the lower adjusting rod, the penetration depth of the fine crushing blade can be flexibly adjusted, which can adapt to production raw materials in different initial states, solves the problem of fixed crushing parameters and narrow range of applicable raw materials in traditional apparatus, and significantly improves the versatility and adaptability of the apparatus.

[0015] (5) A process and apparatus for producing low molecular weight humanized type III collagen, wherein the product centralized flow design improves the convenience of collection: a product flow channel is set at the bottom of the centrifugal separation chamber, and combined with the fixed connection between the components, the low molecular weight humanized type III collagen is centrally guided and flowed out, avoiding product dispersion and residue, facilitating subsequent unified collection and subsequent processing and use, and improving the practicality and ease of operation of the apparatus.

[0016] (6) A process and apparatus for producing low molecular weight humanized type III collagen. The low molecular weight humanized type III collagen produced by this apparatus is prepared by gene editing technology. Specifically, it is obtained by using a gene editing device to design a gene cutting enzyme to cut a specific fragment of human gene, and then expressing the gene fragment corresponding to 458 amino acids in SEQ ID NO:1. The diameter of the expressed molecule is 1.4 nm and the length is 280 nm. This molecule has stronger transdermal performance and can penetrate the basal layer through sweat glands to reach the dermis. It can also be rapidly distributed on the wound and achieve coverage without dead angles, effectively promoting granulation tissue growth and crawling, accelerating wound healing, and expanding the application scenarios and effects of the product in the fields of medical aesthetics and biomedical materials. Attached Figure Description

[0017] Figure 1 This is a cross-sectional view of the overall structure of the present invention; Figure 2 This is a schematic diagram of the overall appearance of the invention; Figure 3 This is a schematic diagram of the built-in crushing mechanism of the present invention; Figure 4 This is a schematic diagram of the graded crushing blade assembly of the present invention; Figure 5 This is a schematic diagram of the built-in centrifugal mechanism of the present invention.

[0018] In the diagram: 1. Raw material feed ring; 2. Raw material crushing assembly; 21. Crushing chamber shell; 22. Built-in crushing mechanism; 221. Drive turntable; 222. Crushing adjustment seat; 223. Upper adjustment rod; 224. Lower adjustment rod; 225. Grading crushing blade assembly; 2251. Blade assembly mounting plate; 2252. Upper adjustment slider; 2253. Lower adjustment slider; 2254. Refining crushing blade; 226. Preliminary crushing blade; 3. Material transition assembly; 31. Transition guide block; 32. Variable diameter transition chamber; 4. Centrifugal separation assembly; 41. Centrifugal chamber shell; 42. Built-in centrifugal mechanism; 421. Centrifugal turntable; 422. Centrifugal separation chamber; 423. Product guide channel; 5. Product outlet. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example: Please refer to Figure 1-5 A process and apparatus for producing low molecular weight humanized type III collagen includes a raw material feeding ring 1 with a circular shell for passing raw materials. A raw material crushing assembly 2 is fixedly connected below the raw material feeding ring 1. A material transition assembly 3 is fixedly connected below the raw material crushing assembly 2. A centrifugal separation assembly 4 is fixedly connected below the material transition assembly 3. A product outlet 5 is fixedly connected below the centrifugal separation assembly 4. The raw material crushing assembly 2 is responsible for thoroughly crushing the raw materials. The raw material crushing assembly 2 includes a crushing chamber shell 21 and a built-in crushing mechanism 22. The crushing chamber shell 21 is a cylindrical shell, and a [missing information - likely a component or structure] is disposed within the crushing chamber shell 21. The built-in crushing mechanism 22 and the material transition component 3 include a transition guide block 31 and a variable diameter transition cavity 32. The transition guide block 31 is a cylindrical block with a hole at its center. The variable diameter transition cavity 32 is composed of a symmetrical frustum-shaped shell and a cylindrical shell fixedly connected to each other. The centrifugal separation component 4 is responsible for centrifuging the processing liquid flowing out of the material transition component 3. The centrifugal separation component 4 includes a centrifugal chamber shell 41 and a built-in centrifugal mechanism 42. The centrifugal chamber shell 41 is a cylindrical shell, and the built-in centrifugal mechanism 42 is disposed inside the centrifugal chamber shell 41. The bottom of the centrifugal chamber shell 41 is fixedly connected to the product outlet 5.

[0021] The built-in crushing mechanism 22 includes a drive turntable 221, which has a built-in rotary motor. A crushing adjustment seat 222 is fixedly connected to the bottom of the drive turntable 221. The crushing adjustment seat 222 has a built-in drive motor. Three upper adjustment rods 223 and lower adjustment rods 224 are arranged around the crushing adjustment seat 222. The three upper adjustment rods 223 and lower adjustment rods 224 are arranged in a ring. The upper adjustment rods 223 and lower adjustment rods 224 are on the same vertical line. The lower adjustment rods 224 are located below the upper adjustment rods 223. The tail ends of the upper adjustment rods 223 and lower adjustment rods 224 are fixedly connected to a grading crushing blade assembly 225. A preliminary crushing blade 226 is also fixedly connected to the bottom end of the crushing adjustment seat 222.

[0022] The graded crushing blade assembly 225 includes a blade assembly mounting plate 2251, which is a rectangular plate. The blade assembly mounting plate 2251 is fixedly connected from top to bottom to an upper adjusting slider 2252, a lower adjusting slider 2253, and a fine crushing blade 2254. The upper adjusting slider 2252 is movably sleeved in the upper adjusting rod 223 and can move laterally within the upper adjusting rod 223. The lower adjusting slider 2253 is movably sleeved in the lower adjusting rod 224 and can move laterally within the lower adjusting rod 224.

[0023] There are three fine pulverizing blades 2254, and the three fine pulverizing blades 2254 are evenly distributed.

[0024] The built-in centrifugal mechanism 42 includes a centrifugal frame 421, which is a ring with four fan-shaped holes. A circular hole is opened at the center of the centrifugal frame 421. The centrifugal frame 421 is fixedly connected to the preliminary crushing blade 226. Centrifugal separation chambers 422 are fixedly connected to the fan-shaped holes on the centrifugal frame 421. The centrifugal separation chambers 422 are fan-shaped shells. A product guide groove 423 is fixedly connected to the bottom of the centrifugal separation chambers 422.

[0025] The drive turntable 221 is frustum-shaped and its outer wall is smooth.

[0026] A process for producing low molecular weight humanized type III collagen, characterized by comprising the following steps: Step 1: Gene editing to prepare specific raw materials. A gene-cutting enzyme for a specific fragment is designed using a gene editing device. The gene-cutting enzyme is used to cut the human gene to obtain a specific gene fragment. The gene fragment corresponding to 458 amino acids in SEQ ID NO:1 is extracted and expressed to obtain the target precursor raw material. The diameter of the precursor raw material expression molecule is 1.4 nm and the length is 280 nm.

[0027] Step 2: Raw material pretreatment feeding. The collagen production raw materials are introduced into the crushing chamber shell 21 through the raw material feeding ring 1. The raw materials are dispersed and slide down around the outer wall of the frustum-shaped and smooth drive turntable 221 to avoid accumulation. Step 3: Precise pulverization and grading. Start the built-in rotary motor of the drive turntable 221 to drive the built-in pulverizing mechanism 22 to rotate as a whole. The primary pulverizing blade 226 rotates synchronously to perform primary pulverization of the raw material. Then start the drive motor built-in of the pulverizing adjustment seat 222 to drive the upper adjustment slider 2252 to move laterally within the upper adjustment rod 223 and the lower adjustment slider 2253 within the lower adjustment rod 224. Adjust the penetration depth of the three evenly spaced fine pulverizing blades 2254 to perform secondary fine pulverization of the primary pulverized raw material to obtain a raw material processing liquid with uniform particle size. Step 4: Smooth material transition. The raw material processing liquid flows into the transition guide block 31 through the connection channel between the raw material crushing component 22 and the material transition component 3. After passing through its central hole, it enters the variable diameter transition cavity 32. The variable diameter structure achieves a smooth flow rate transition, avoiding residue or splashing. Step 5: Power-driven centrifugal separation. With the rotational power of the drive turntable 221, the centrifugal turntable 421 and the four fan-shaped centrifugal separation chambers 422 are driven to rotate at high speed through the fixed connection between the preliminary crushing blade 226 and the centrifugal frame 421. The raw material processing liquid undergoes centrifugal motion in the centrifugal separation chambers 422 to separate small molecular weight humanized type III collagen. Step 6: Product collection. The sorted low molecular weight humanized type III collagen is guided by the product guide channel 423 at the bottom of the centrifugal separation chamber 422 and flows out through the fixed connection channel between the centrifugal chamber shell 41 and the product outlet 5, thus completing the product collection.

[0028] The working principle of this invention is as follows: The first step involves preparing the raw material using a gene editing device: a gene-cutting enzyme with a specific fragment is designed, and this enzyme is used to cut the human gene to obtain a specific gene fragment. The gene fragment corresponding to 458 amino acids in SEQ ID NO:1 is extracted and expressed to obtain the target precursor raw material with a diameter of 1.4 nm and a length of 280 nm. The 1.4 nm diameter molecule has stronger transdermal performance, penetrating the basal layer through sweat glands to reach the dermis. This molecule can achieve rapid distribution and thorough coverage of the wound, promoting granulation tissue growth and accelerating healing.

[0029] In the second step, the raw materials enter the device through the raw material feeding ring 1. Since the raw material feeding ring 1 is fixedly connected to the raw material crushing component 2, the raw materials flow into the crushing chamber shell 21 of the raw material crushing component 2. The drive turntable 221 inside the raw material crushing component 2 is frustum-shaped and has a smooth outer wall. The raw materials slide down along its outer wall, avoiding the problem of accumulation. At the same time, the rotary motor built into the drive turntable 221 starts. Since the bottom of the drive turntable 221 is fixedly connected to the crushing adjustment seat 222, the rotary motor drives the built-in crushing mechanism 22 to rotate synchronously. The preliminary crushing blade 226 performs preliminary crushing of the raw materials with this rotation. The synchronous rotation linkage makes the crushing force on the raw materials more uniform, effectively improving the efficiency of the pre-processing.

[0030] Third, the drive motor built into the crushing adjustment seat 222 is started, which drives the upper adjustment slider 2252 to move laterally within the upper adjustment rod 223 and the lower adjustment slider 2253 within the lower adjustment rod 224. Through this linkage action, the penetration depth of the fine crushing blade 2254 can be flexibly adjusted to adapt to the initial crushing raw materials in different states, so that the raw materials are crushed more thoroughly and the particle size is more uniform, laying a good foundation for subsequent processes.

[0031] In the fourth step, the raw material processing liquid, after being finely crushed by the raw material crushing component 2, flows into the material transition component 3 through the fixed connection between the raw material crushing component 2 and the material transition component 3. It first passes through the hole in the center of the transition guide block 31, and then enters the variable diameter transition cavity 32 to complete the flow transition. The structural design of the material transition component 3 is linked with the flow of the processing liquid, so that the flow rate of the processing liquid is smoothly transitioned, avoiding the problems of raw material residue or processing liquid splashing caused by sudden changes in flow rate.

[0032] In the fifth step, after the processing liquid flows out from the material transition component 3, it enters the centrifugal chamber shell 41 of the centrifugal separation component 4 through the fixed connection between the material transition component 3 and the centrifugal separation component 4. Since the centrifugal frame 421 of the built-in centrifugal mechanism 42 is fixedly connected to the preliminary crushing blade 226, when the drive turntable 221 rotates and drives the preliminary crushing blade 226 to rotate, it simultaneously drives the centrifugal frame 421 and the centrifugal separation chamber 422 fixed thereto to rotate at high speed. The linkage method of synchronously driving the operation of the centrifugal separation component 4 with the rotation power of the raw material crushing component 2 reduces the setting of additional power components and reduces the cost of the device. At the same time, the processing liquid undergoes centrifugal motion with high speed rotation in the centrifugal separation chamber 422, efficiently separating small molecular weight humanized type III collagen and retaining it in the centrifugal separation chamber 422, thus improving the separation accuracy.

[0033] In the sixth step, the low molecular weight humanized type III collagen retained in the centrifugal separation chamber 422 is aggregated into the product guide channel 423 through the fixed connection between the centrifugal separation chamber 422 and the product guide channel 423, and then flows out from the product outlet 5 through the fixed connection between the centrifugal separation component 4 and the product outlet 5. The centralized guidance of the product guide channel 423 and the linkage of the various structures facilitate the unified collection and use of the target collagen in the future, thus improving the practicality of the device.

[0034] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A low molecular weight humanized type III collagen production device, comprising a raw material feeding ring (1), the raw material feeding ring (1) being a circular shell through which raw materials can pass, a raw material crushing assembly (2) fixedly connected below the raw material feeding ring (1), a material transition assembly (3) fixedly connected below the raw material crushing assembly (2), a centrifugal separation assembly (4) fixedly connected below the material transition assembly (3), and a product outlet (5) fixedly connected below the centrifugal separation assembly (4), characterized in that: The raw material crushing assembly (2) is responsible for fully crushing the raw material. The raw material crushing assembly (2) includes a crushing chamber shell (21) and a built-in crushing mechanism (22). The crushing chamber shell (21) is a cylindrical shell, and the built-in crushing mechanism (22) is provided inside the crushing chamber shell (21). The material transition assembly (3) includes a transition guide block (31) and a variable diameter transition cavity (32). The transition guide block (31) is a cylindrical block, and a hole is opened at the center of the transition guide block (31). The transition chamber (32) is composed of a symmetrical frustum-shaped shell and a cylindrical shell fixedly connected between them. The centrifugal separation component (4) is responsible for centrifuging the processing liquid flowing out from the material transition component (3). The centrifugal separation component (4) includes a centrifugal chamber shell (41) and a built-in centrifugal mechanism (42). The centrifugal chamber shell (41) is a cylindrical shell. The built-in centrifugal mechanism (42) is provided inside the centrifugal chamber shell (41). The bottom of the centrifugal chamber shell (41) is fixedly connected to the product outlet (5).

2. The apparatus for producing low molecular weight humanized type III collagen according to claim 1, characterized in that: The built-in crushing mechanism (22) includes a drive turntable (221), which has a built-in rotary motor. A crushing adjustment seat (222) is fixedly connected to the bottom of the drive turntable (221). The crushing adjustment seat (222) has a built-in drive motor. Three upper adjustment rods (223) and lower adjustment rods (224) are arranged around the crushing adjustment seat (222). The three upper adjustment rods (223) and the lower adjustment rods (224) are arranged in a ring.

3. The apparatus for producing low molecular weight humanized type III collagen according to claim 2, characterized in that: The upper adjusting rod (223) and the lower adjusting rod (224) are on the same vertical line. The lower adjusting rod (224) is located below the upper adjusting rod (223). The tail ends of the upper adjusting rod (223) and the lower adjusting rod (224) are fixedly connected to the grading crushing blade assembly (225). The bottom end of the crushing adjusting seat (222) is also fixedly connected to the preliminary crushing blade (226).

4. The apparatus for producing low molecular weight humanized type III collagen according to claim 3, characterized in that: The graded crushing blade assembly (225) includes a blade assembly mounting plate (2251), which is a rectangular plate. The blade assembly mounting plate (2251) is fixedly connected to an upper adjusting slider (2252), a lower adjusting slider (2253), and a fine crushing blade (2254) from top to bottom.

5. The apparatus for producing low molecular weight humanized type III collagen according to claim 4, characterized in that: The upper adjusting slider (2252) is movably sleeved inside the upper adjusting rod (223), and the upper adjusting slider (2252) can move laterally inside the upper adjusting rod (223). The lower adjusting slider (2253) is movably sleeved inside the lower adjusting rod (224), and the lower adjusting slider (2253) can move laterally inside the lower adjusting rod (224).

6. The apparatus for producing low molecular weight humanized type III collagen according to claim 5, characterized in that: There are three fine pulverizing blades (2254), and the three fine pulverizing blades (2254) are evenly distributed.

7. The apparatus for producing low molecular weight humanized type III collagen according to claim 6, characterized in that: The built-in centrifugal mechanism (42) includes a centrifugal frame (421), which is a ring with four fan-shaped holes. A circular hole is opened at the center of the centrifugal frame (421). The centrifugal frame (421) is fixedly connected to the preliminary crushing blade (226). Centrifugal separation chambers (422) are fixedly connected to the fan-shaped holes on the centrifugal frame (421). The centrifugal separation chambers (422) are fan-shaped shells. A product guide groove (423) is fixedly connected to the bottom of the centrifugal separation chambers (422).

8. The low molecular weight humanized type III collagen production device according to claim 7, wherein the driving turntable (221) is frustum-shaped and the outer wall of the driving turntable (221) is smooth.

9. A low molecular weight humanized type III collagen production apparatus and a low molecular weight humanized type III collagen production process according to any one of claims 1-8, characterized in that, The process includes the following steps: Step 1: Gene editing to prepare specific raw materials. A gene-cutting enzyme for a specific fragment is designed using a gene editing device. The gene-cutting enzyme is used to cut the human gene to obtain a specific gene fragment. The gene fragment corresponding to 458 amino acids in SEQ ID NO:1 is extracted and expressed to obtain the target precursor raw material. The diameter of the precursor raw material expression molecule is 1.4 nm and the length is 280 nm. Step 2: Raw material pretreatment feeding. The collagen production raw materials are introduced into the crushing chamber shell (21) through the raw material feeding ring (1). The raw materials are dispersed and slide down around the outer wall of the frustum-shaped and smooth drive turntable (221) to avoid accumulation. Step 3: Graded and precise crushing. Start the built-in rotary motor of the drive turntable (221) to drive the built-in crushing mechanism (22) to rotate as a whole. The initial crushing blade (226) rotates synchronously to crush the raw material. Then start the built-in drive motor of the crushing adjustment seat (222) to drive the upper adjustment slider (2252) to move laterally in the upper adjustment rod (223) and the lower adjustment slider (2253) to move laterally in the lower adjustment rod (224). Adjust the penetration depth of the three evenly spaced fine crushing blades (2254) to perform secondary fine crushing on the raw material after initial crushing to obtain a raw material processing liquid with uniform particle size. Step 4: Smooth material transition. The raw material processing liquid flows into the transition guide block (31) through the connection channel between the raw material crushing component (22) and the material transition component (3), and enters the variable diameter transition chamber (32) after passing through its central hole. The variable diameter structure achieves a smooth flow rate transition, avoiding residue or splashing. Step 5: Power-driven centrifugal separation. With the rotational power of the drive turntable (221), the centrifugal turntable (421) and the four fan-shaped centrifugal separation chambers (422) are driven to rotate at high speed through the fixed connection between the preliminary crushing blade (226) and the centrifugal rack (421). The raw material processing liquid undergoes centrifugal motion in the centrifugal separation chamber (422) to separate small molecular weight humanized type III collagen. Step 6: Collect the product. The sorted low molecular weight humanized type III collagen is guided by the product guide channel (423) at the bottom of the centrifugal separation chamber (422) and flows out through the fixed connection channel between the centrifugal chamber shell (41) and the product outlet (5) to complete the product collection.

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