Bactericidal composition containing chlorothalonil and application thereof

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a chlorothalonil-containing bactericidal composition and application thereof, the bactericidal composition comprises an active component A and an active component B, the active component A is Cyclobutrifluram, the active component B is chlorothalonil, and the mass ratio of the active component A to the active component B is 1: 56-56: 1. The bactericidal composition disclosed by the invention has a relatively good control effect on plant diseases such as gibberellic disease and fusarium wilt, can slow down the generation and development of drug resistance of pathogenic bacteria, reduces the use dosage of pesticides, and has an obvious yield keeping effect.

Description

Technical Field

[0001] This invention belongs to the field of pesticide sterilization technology, and discloses a bactericidal composition containing chlorothalonil and its application. Background Technology

[0002] Diseases affecting cucurbit vegetables are a significant factor severely restricting the development of their industry. Fusarium wilt, also known as wilt disease, is a global disease and a common disease of major cucurbits such as cucumber, winter melon, wax gourd, bitter melon, cantaloupe, and watermelon, and is prevalent in major cucurbit-growing areas in China. The Fusarium fungus that causes Fusarium wilt in cucurbits has at least eight specialized serotypes. Currently, six specialized serotypes have been reported in my country: watermelon, cucumber, cantaloupe, winter melon, gourd, and bitter melon.

[0003] Fusarium head blight (FHB) is a significant fungal disease affecting wheat production, substantially impacting yield and quality. It is caused by several species of Fusarium fungi, most notably *Fusarium graminearum* and *Fusarium xanthoides*. FHB is a climate-dependent disease, with the highest infectivity typically occurring in the early flowering stage. Infection can occur at the root, stem base, and ear, causing root rot, stem base rot, and ear rot. FHB is one of the most serious diseases causing wheat yield reduction. The fungus secretes DON toxin, which is highly harmful to humans and animals.

[0004] In response to the above-mentioned diseases, this invention studies the control effects of Cyclobutrifluram and chlorothalonil on wheat scab and cucurbit wilt. Indoor experiments determined the optimal ratio of Cyclobutrifluram and chlorothalonil to enhance their efficacy against wheat scab and cucumber wilt. Field trials further verified that the combination of these two agents has a good control effect on wheat scab and cucumber wilt, providing a new control agent for these diseases with significant yield protection. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a fungicide composition containing chlorothalonil. This fungicide composition exhibits excellent control effects against plant diseases, particularly showing a significant synergistic effect against wheat scab and cucurbit wilt. It can effectively slow disease progression, reduce pathogen resistance development, decrease pesticide dosage, and significantly improve yield protection.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a bactericidal composition containing chlorothalonil, wherein the bactericidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram, and active ingredient B is chlorothalonil, and the mass ratio of active ingredient A to active ingredient B is 1:56 to 56:1, such as 1:56, 1:40, 1:48, 1:32, 1:30, 1:16, 1:15, 1:9, 1:8, 4:1, 1:3, 1:1, 3:1, 8:1, 9:1, 16:1, 18:1, 27:1, 32:1, 36:1, 48:1, 56:1, or any value between the above values;

[0007] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:48 to 36:1, such as 1:48, 1:32, 1:30, 1:16, 1:15, 1:9, 1:8, 4:1, 1:3, 1:1, 3:1, 8:1, 9:1, 16:1, 18:1, 27:1, 32:1, 36:1 or any value between the above.

[0008] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:48 to 32:1, such as 1:48, 1:32, 1:30, 1:16, 1:15, 1:9, 1:8, 4:1, 1:3, 1:1, 3:1, 8:1, 9:1, 16:1, 18:1, 27:1, 32:1 or any value between these values;

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:30 to 32:1, such as 1:30, 1:16, 1:15, 1:9, 1:8, 4:1, 1:3, 1:1, 3:1, 8:1, 9:1, 16:1, 18:1, 27:1, 32:1 or any value between these values;

[0010] Furthermore, the total weight of the bactericidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the bactericidal composition.

[0011] Furthermore, in addition to the active ingredient, the bactericidal composition also contains pesticide-acceptable auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

[0012] Furthermore, the bactericidal composition can be prepared into any formulation acceptable in the pesticide field, wherein the formulation is selected from granules, balls, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, soluble granules, soluble solutions, soluble gels, oils, spreading oils, emulsions, latexes, dispersible liquids, pastes, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, or suspension emulsions;

[0013] Furthermore, the bactericidal composition formulation is in the form of a suspension, water-dispersible granules, or wettable powder.

[0014] The present invention also discloses the application of the bactericidal composition described above for the prevention and control of plant diseases.

[0015] Furthermore, the plant disease mentioned is either Fusarium wilt of the Cucurbitaceae family or Fusarium head blight of wheat;

[0016] Furthermore, the cucurbitaceous wilt disease mentioned refers to cucumber wilt, melon wilt, or watermelon wilt.

[0017] The present invention also discloses a method for preventing and controlling plant diseases, wherein the bactericidal composition according to any one of claims 1-7 is applied to the medium in which the disease occurs.

[0018] The present invention has the following beneficial effects:

[0019] The bactericidal composition of the present invention has excellent control effect on plant diseases caused by Fusarium pathogens. Within a suitable ratio range of the two compounds, it has a significant synergistic effect on wheat scab and cucurbit wilt, a long-lasting effect, is safe for crops, and effectively reduces the amount of pesticides used, resulting in significant yield protection and promotion effects. Detailed Implementation

[0020] Formulation preparation example

[0021] Preparation Example 1: 24% Cyclobutrifluram·chlorothalonil suspension (1:3)

[0022] Formula: 6% Cyclobutrifluram, 18% Chlorothalonil, 1% Ethylene glycol oxyethylene polyoxypropylene ether, 3% Phenethylphenol polyoxyethylene ether phosphate, 1% Sodium lignosulfonate, 0.5% Sodium polyacrylate, 0.2% Xanthan gum, 1% Magnesium aluminum silicate, 5% Ethylene glycol, 0.1% Sodium sorbate, 0.5% Organosilicone defoamer, deionized water to make up the balance;

[0023] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0024] Preparation Example 2: 36% Cyclobutrifluram·chlorothalonil suspension (1:8)

[0025] Formula: 4% Cyclobutrifluram, 32% Chlorothalonil, 1% Gelbert alcohol polyoxyethylene ether, 1% Naphthalene sulfonate formaldehyde condensate, 3% Arylphenol polyoxyethylene ether phosphate, 2% Phenethylphenol polyoxyethylene polyoxypropylene ether, 0.2% Xanthan gum, 1% Magnesium aluminum silicate, 5% Propylene glycol, 0.2% Potassium benzoate, 0.5% Organosilicone defoamer, deionized water to make up the balance;

[0026] Preparation method: Same as in preparation example 1.

[0027] Preparation Example 3: 40% Cyclobutrifluram·chlorothalonil water-dispersible granules (1:9)

[0028] Formula: 4% Cyclobutrifluram, 36% Chlorothalonil, 11% Sodium lignosulfonate, 5% Sodium polycarboxylate, 2% Sodium dodecyl sulfate, 5% precipitated silica, 30% starch, and kaolin to make up the balance;

[0029] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0030] Preparation Example 4: 33% Cyclobutrifluram·chlorothalonil water-dispersible granules (10:1)

[0031] Formula: 30% Cyclobutrifluram, 3% Chlorothalonil, 8% Naphthalenesulfonate formaldehyde condensate, 3% Sodium alkylnaphthalenesulfonate, 3% Sodium dodecyl sulfate, 10% Ammonium sulfate, starch to make up the balance;

[0032] Preparation method: Same as in preparation example 3.

[0033] Preparation Example 5: 52% Cyclobutrifluram·chlorothalonil wettable powder (1:1)

[0034] Formula: 26% Cyclobutrifluram, 26% Chlorothalonil, 13% Sodium lignosulfonate, 8% Sodium polycarboxylate, 10% Starch, 5% Silica, Kaolin to make up the balance;

[0035] Preparation method: According to the formulation ratio in the example, the active ingredients are added to the carrier, and surfactants and other functional additives are added thereto. After mixing, the mixture is pulverized by air jet and then mixed again to obtain a wettable powder product.

[0036] Preparation Example 6: 32% Cyclobutrifluram·chlorothalonil wettable powder (15:1)

[0037] Formula: 30% Cyclobutrifluram, 2% Chlorothalonil, 10% Sodium lignosulfonate, 2% Sodium dodecyl sulfate, 5% Sodium polycarboxylate, 10% Starch, 5% Silica, Kaolin to make up the balance;

[0038] Preparation method: Same as in preparation example 5.

[0039] Preparation Example 7: 4% Cyclobutrifluram·chlorothalonil seed treatment suspension (4:1)

[0040] Formula: 3.2% Cyclobutrifluram, 0.8% Chlorothalonil, 1% Block Polyether, 1% Sodium Succinate Sulfonate, 2% Alkylphenol Polyoxyethylene Ether Phosphate, 2% Sodium Polycarboxylate, 1% Magnesium Aluminum Silicate, 0.2% Xanthan Gum, 4% Polyacrylic Acid Emulsion, 4% Rose Red Pigment, 5% Glycerol, 0.5% Silicone Defoamer, 0.3% Sodium Sorbitate, Deionized Water to make up the balance;

[0041] Preparation method: The active ingredients, additives and water are mixed and stirred evenly under high shear rate according to the formula ratio, and then sanded for 2.5 hours in a sand mill to make the average particle size reach 1-5 micrometers, thus obtaining the seed treatment suspension.

[0042] Preparation Example 8: 2.7% Cyclobutrifluram·chlorothalonil seed treatment suspension (1:8)

[0043] Formula: 0.3% Cyclobutrifluram, 2.4% Chlorothalonil, 1% Fatty alcohol polyoxyethylene ether, 1% Polyoxyethylene dehydrated sorbitan monooleate, 2% Sodium lignosulfonate, 3% Tristyrene-phenol ethoxylated phosphate, 1% Polyacrylic acid emulsion, 0.2% Xanthan gum, 4% Rose red pigment, 5% Ethylene glycol, 1% Magnesium aluminum silicate, 0.5% Organosilicon defoamer, 0.3% Sodium benzoate, deionized water to make up the balance;

[0044] Preparation method: Same as in preparation example 7.

[0045] Indoor bioactivity test

[0046] Example 1: Indoor bioactivity test of Cyclobutrifluram and chlorothalonil in controlling wheat scab

[0047] Experimental basis: The experiment was conducted in accordance with NY / T 1156.2-2006, the Agricultural Industry Standard of the People's Republic of China, "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Tests for Inhibition of Mycelial Growth of Pathogenic Fungi - Plate Method".

[0048] Experimental target: Fusarium graminearum.

[0049] Test reagents: Chlorothalonil technical grade and Cyclobutrifluram technical grade.

[0050] Drug preparation: Dissolve the original drug in a suitable solvent, then dilute with 0.1% Tween 80 aqueous solution to prepare single-dose stock solutions. Design different ratios according to the purpose of mixing and drug activity. Prepare the required series of mass concentrations for each single drug and each group of mixed solutions.

[0051] Chemical treatment: Under aseptic conditions, pre-melted and sterilized PDA medium was quantitatively added to sterile Erlenmeyer flasks according to the experimental treatment. From low to high concentration, 10 mL of each prepared treatment solution was quantitatively pipetted and added to the Erlenmeyer flasks, thoroughly mixed, and then poured into petri dishes to prepare the corresponding concentration of drug-containing plates. A 0.1% Tween 80 aqueous solution without added drug was set up as a blank control. Each treatment was repeated in quadruplicate.

[0052] Inoculation: Under aseptic conditions, use a sterilized punch to cut off a mycelial cake from the edge of the pre-cultured Fusarium graminearum. Inoculate the mycelial cake into the center of the drug-containing plate with an inoculator, cover with the cap, and place in a constant temperature and light incubator at 26°C for cultivation.

[0053] Data collection: The growth of pathogenic fungal hyphae was investigated based on the growth of hyphae in the blank control culture dishes. The diameter of the colonies was measured in centimeters (cm) using a ruler. The diameter of each colony was measured once using the cross-sectional method, and the average value was taken. The original data of all replicates for each treatment were recorded.

[0054] Data statistics and analysis: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated, in percentage (%). The calculation results were retained to two decimal places.

[0055] D = D1 - D2

[0056] In the formula:

[0057] D – Colony growth diameter;

[0058] D1—colony diameter;

[0059] D2 – Diameter of the mushroom cake.

[0060]

[0061] In the formula:

[0062] I – Mycelial growth inhibition rate;

[0063] D0—Correlation diameter of the blank control group;

[0064] D T — Diameter of colonies grown after chemical treatment.

[0065] Analyze using the DPS statistical analysis system to determine EC 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0066] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0067] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0068]

[0069] In the formula:

[0070] ATI – Actual Measured Toxicity Index of Mixtures;

[0071] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0072] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0073] TTI = TI A ×P A +TI B ×P B

[0074] In the formula:

[0075] TTI – Theoretical Toxicity Index of Mixtures;

[0076] TI A —A. Toxicity index of drug A;

[0077] P A—Percentage content of drug A in the mixture, expressed as percentage (%);

[0078] TI B —Toxicity index of drug B;

[0079] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0080]

[0081] In the formula:

[0082] CTC – Cotoxicity Coefficient;

[0083] ATI – Actual Measured Toxicity Index of Mixtures;

[0084] TTI – Theoretical Toxicity Index of Mixtures.

[0085] The test results are shown in the table below:

[0086] Table 1. Results of indoor bioactivity tests of Cyclobutrifluram and chlorothalonil in controlling wheat scab.

[0087]

[0088]

[0089] Table 1 shows the results of the indoor efficacy test. It can be seen that the combination of Cyclobutrifluram and chlorothalonil in a mass ratio of 1:30 to 36:1 has a synergistic effect on wheat scab. When the mass ratio of the two is 1:15 to 18:1, the co-toxicity coefficient against wheat scab is greater than 130, and the synergistic effect is obvious. When the mass ratio of the two is 1:9 to 9:1, the co-toxicity coefficient is greater than 150, and the synergistic effect is significant.

[0090] Example 2: Indoor bioactivity test of Cyclobutrifluram and chlorothalonil in controlling cucumber wilt disease

[0091] Experimental basis: The experiment was conducted in accordance with NY / T 1156.2-2006, the Agricultural Industry Standard of the People's Republic of China, "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Tests for Inhibition of Mycelial Growth of Pathogenic Fungi - Plate Method".

[0092] Test subject: Fusarium oxyspoum f.sp.cucumerinum Owen.

[0093] Test reagents: cyclobutrifluram technical grade, chlorothalonil technical grade.

[0094] Reagent preparation: Dissolve the active ingredient in a suitable solvent first, then dilute with a 0.1% Tween 80 aqueous solution. Prepare five concentration gradients for each reagent, ready for use.

[0095] Experimental method: In a clean bench, 6 mL of the prepared drug solution was poured into a sterile Erlenmeyer flask, and 54 mL of potato dextrose agar (PDA) medium at about 50°C was added. After shaking well, the solution was poured into a 9 cm diameter Petri dish to prepare the corresponding concentration of toxic medium. A 0.1% Tween 80 aqueous solution without the drug was set as a blank control. Each treatment was repeated 4 times.

[0096] Using a 6mm diameter punch, pre-cultured bacterial cultures were used to cut bacterial blocks from the edge of colonies. These blocks were then transferred to the center of a pre-prepared virus-containing culture medium using an inoculation needle and incubated at 25°C. Each treatment was repeated four times. The mycelial growth of the pathogen in each treatment was investigated based on the mycelial growth observed in the blank control dishes. Colony diameters were measured using a ruler, with each colony measured once using the cross-sectional method. The average value was recorded, and the original data for all replicates of each treatment were recorded.

[0097] Data statistics and analysis: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated, in percentage (%). The calculation results were retained to two decimal places.

[0098] D = D1 - D2

[0099] In the formula:

[0100] D – Colony growth diameter;

[0101] D1—colony diameter;

[0102] D2 – Diameter of the mushroom cake.

[0103]

[0104] In the formula:

[0105] I – Mycelial growth inhibition rate;

[0106] D0—Correlation diameter of the blank control group;

[0107] D T — Diameter of colonies grown after chemical treatment.

[0108] The EC was calculated using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0109] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0110] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0111]

[0112] In the formula:

[0113] ATI – Actual Measured Toxicity Index of Mixtures;

[0114] S—EC of standard reagent 50 The unit is milligrams per liter (mg / L);

[0115] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0116] TTI = TI A ×P A +TI B ×P B

[0117] In the formula:

[0118] TTI – Theoretical Toxicity Index of Mixtures;

[0119] TI A —A. Toxicity index of drug A;

[0120] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0121] TI B —Toxicity index of drug B;

[0122] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0123]

[0124] In the formula:

[0125] CTC – Cotoxicity Coefficient;

[0126] ATI – Actual Measured Toxicity Index of Mixtures;

[0127] TTI – Theoretical Toxicity Index of Mixtures.

[0128] The test results are shown in the table below:

[0129] Table 2. Results of indoor bioactivity tests of Cyclobutrifluram and chlorothalonil in controlling cucumber wilt.

[0130]

[0131] Table 2 shows the results of the indoor bioactivity assay. The combination of Cyclobutrifluram and chlorothalonil exhibited good inhibitory effects on the mycelial growth of cucumber wilt fungus. When the mass ratio of Cyclobutrifluram to chlorothalonil was 1:48–32:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:32–16:1, the co-toxicity coefficient was greater than 130, showing a significant synergistic effect; and when the mass ratio was 1:16–16:1, the co-toxicity coefficient was greater than 140, showing a remarkable synergistic effect.

[0132] Field efficacy trials

[0133] Example 3: Field efficacy trial of pesticides for controlling wheat scab

[0134] The experiment was conducted in accordance with NY / T 1464.15-2007 "Guidelines for Field Efficacy Tests of Pesticides - Part 15: Fungicides for the Control of Wheat Fusarium Head Blight".

[0135] Experimental site: The experiment was conducted in Yangwang Village, Xinjiang County, Yuncheng City, Shanxi Province. The soil at the experimental site is clay with a high organic matter content and good drainage and irrigation conditions.

[0136] Experimental crop: Wheat (Jinmai 84).

[0137] Experimental target: Wheat scab.

[0138] Experimental Design: The experiment included 5 chemical treatments and 1 water control, with each treatment replicated 4 times, for a total of 24 plots, each plot being 50 m². 2 The experimental agent, control agent, and blank control plots were arranged in a randomized block design, with a 1m isolation zone set up between each plot to avoid mutual interference between the drug administrations.

[0139] Experimental method: The pesticide was applied on April 19, 2022, using a backpack electric sprayer. A second application was carried out 7 days later.

[0140] Investigation method: The investigation was conducted 20 days after the second application of pesticide. Five diagonal sampling points were used for the investigation, with 100 ears of panicle sampled at each point. The panicle area was classified according to the percentage of dead panicle area to the total panicle area. The number of diseased panicles, the disease level, the disease index of diseased panicles, and the control effect were recorded.

[0141] Methods for grading diseased ears:

[0142] Grade 0: Disease-free entire ear of grain;

[0143] Grade 1: The area of ​​dead ears accounts for less than 1 / 4 of the total ear area;

[0144] Grade 3: The area of ​​dead ears accounts for 1 / 4 to 1 / 2 of the total ear area;

[0145] Grade 5: The area of ​​dead ears accounts for 1 / 2 to 3 / 4 of the total ear area;

[0146] Grade 7: The area of ​​withered ears accounts for more than 3 / 4 of the total ear area;

[0147] Methods for calculating drug efficacy:

[0148]

[0149]

[0150]

[0151] Before wheat harvest, a five-point sampling method was used for each plot survey. 100 ears were selected from each treatment area, brought back indoors to dry, and the number of grains per ear and the weight of 1000 grains were counted. The yield per acre was then calculated.

[0152] Safety observation: During the experiment, observe the wheat in each pesticide treatment area at irregular intervals to see if there is any pesticide damage, and record the type and degree of disease.

[0153] The results of the field efficacy trials are shown in the table below:

[0154] Table 3 Results of field efficacy trials for controlling wheat scab.

[0155]

[0156] The field efficacy results in the table above show that the fungicidal composition of this invention has a significant control effect on wheat scab. Furthermore, random visual inspections conducted after application revealed no phytotoxicity in wheat growth and leaves compared to the water control, indicating that the fungicides in each treatment were safe for wheat growth. This fungicidal composition of the present invention effectively controls disease while significantly protecting yield.

[0157] Example 4: Field efficacy test for controlling cucumber wilt disease

[0158] The experiment was conducted in accordance with GB / T 17980.113-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 113: Control of Fusarium Wilt in Cucurbits by Fungicides".

[0159] Experimental location: Vegetable greenhouses in Nanzhuang Village, Luocheng Street, Shouguang City, Shandong Province. Water and fertilizer management was consistent across all experimental treatment areas.

[0160] The experiment was conducted in a vegetable greenhouse in Shouguang City, Shandong Province in November 2022. The pesticide was applied by root irrigation, and the selected cucumber seedlings were of basically the same growth.

[0161] The experiment employed a randomized block design, with each block lasting 28m. 2 Each treatment was repeated four times. The application of the pesticide was carried out at the early stage of cucumber wilt disease. The specific dosage of the active ingredient is shown in the table below.

[0162] Methods for investigating efficacy: Disease incidence was investigated 14 days after application. The total number of cucumber plants and the number of plants infected with Fusarium wilt were investigated in each plot. The disease incidence rate and control effect were calculated using the following formula.

[0163] Cucumber yield survey: Yield measurement was conducted at maturity.

[0164]

[0165]

[0166]

[0167] Table 4 Results of field efficacy trials for controlling cucumber wilt.

[0168]

[0169] Field efficacy studies showed that the combination of Cyclobutrifluram and chlorothalonil was significantly effective in controlling cucumber wilt, and cucumbers in all treatment plots grew normally. The disease incidence rate in the compound treatment group was significantly lower than that in the single-agent control and the blank control.

[0170] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the pesticide composition of the present invention has a good control effect on Fusarium, is safe for target crops, and has significant control efficacy. It is superior to single agents in delaying the development of resistance and prolonging the duration of action, and has a significant yield protection effect.

[0171] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and replaced in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and replacements within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition containing chlorothalonil, characterized in that, The bactericidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram and active ingredient B is chlorothalonil, and the mass ratio of active ingredient A to active ingredient B is 1:56 to 56:

1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:48 to 36:

1.

3. The bactericidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:48 to 32:1; Preferably, the mass ratio of active ingredient A to active ingredient B is 1:30 to 32:

1.

4. The bactericidal composition according to claim 1, characterized in that, The total weight of the bactericidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the bactericidal composition.

5. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredient, the bactericidal composition also contains pesticide-acceptable auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

6. The bactericidal composition according to claim 1, characterized in that, The bactericidal composition can be prepared into any formulation acceptable in the pesticide field, wherein the formulation is selected from granules, balls, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, soluble granules, soluble solutions, soluble gels, oils, spreading oils, emulsions, latexes, dispersible liquids, pastes, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, or suspension emulsions.

7. The bactericidal composition according to claim 6, characterized in that, The bactericidal composition formulation is in the form of a suspension, water-dispersible granules, or wettable powder.

8. The application of the bactericidal composition according to any one of claims 1-7 for the prevention and control of plant diseases.

9. The application according to claim 8, characterized in that, The plant diseases mentioned are Fusarium wilt of the Cucurbitaceae family or Fusarium head blight of wheat; The wilt disease mentioned refers to cucumber wilt, melon wilt, or watermelon wilt.

10. The application according to claim 8, characterized in that, Apply the bactericidal composition according to any one of claims 1-7 to crops where diseases occur.

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