Formula and preparation method of compound animal milk containing natto probiotics

Abstract

The invention provides a compound animal milk formula containing natto probiotics and a preparation method thereof, and belongs to the field of dairy products. The formula comprises the following components in parts by weight: 70-90 parts of animal milk dry powder, 1-30 parts of natto dry powder, 2-8 parts of probiotics and 0.5-2 parts of fructo-oligosaccharide. Dietary nutrients are compounded in the animal milk dry powder; the invention also discloses a preparation method of the formula. The compound animal milk formula containing the natto probiotics and the preparation method of the compound animal milk have the advantages that the activity retention rate of the probiotics is greatly increased, the product stability is remarkably enhanced, the efficacy synergy is outstanding, and the formula adaptability is high.

Description

Technical Field

[0001] This invention belongs to the field of dairy products, specifically referring to a compound animal milk formula containing natto probiotics and its preparation method. Background Technology

[0002] Milk-based probiotic products are widely favored by consumers because they combine the nutrition of milk with the health benefits of probiotics. However, existing milk-based probiotic products have many technical defects, which restrict their development and application.

[0003] In existing milk-based probiotic products, probiotics are easily eroded by stomach acid and bile in the milk environment, resulting in low activity retention rates. After oral administration, less than 10% of probiotics survive in the acidic environment of the stomach, failing to effectively colonize the intestines. Ordinary probiotics have poor compatibility with milk proteins and fats, easily leading to flocculation and stratification, causing protein denaturation and flavor deterioration within the product's shelf life, affecting product stability and taste. Most existing formulas on the market are simple compound probiotics, failing to fully utilize the synergistic effect of nattokinase and probiotics, resulting in insufficient targeted effects on intestinal health and vascular protection, and failing to achieve the dual health benefits of intestinal regulation and vascular protection. Summary of the Invention

[0004] In view of the above situation and to overcome the defects of the prior art, the purpose of the present invention is to provide a compound animal milk formula containing natto probiotics and its preparation method, so as to at least partially solve the problems mentioned in the background art.

[0005] The technical solution adopted by the present invention is as follows: The present invention proposes a compound animal milk formula containing natto probiotics, comprising the following components in parts by weight: 70-90 parts of animal milk powder, 1-30 parts of natto powder, 2-8 parts of probiotics and 0.5-2 parts of fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

[0006] Furthermore, a compound animal milk formula containing natto probiotics includes the following components in parts by weight: 80 parts animal milk powder, 15.5 parts natto powder, 5 parts probiotics, and 1.25 parts fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

[0007] Furthermore, a compound animal milk formula containing natto probiotics includes the following components in parts by weight: 70 parts animal milk powder, 1 part natto powder, 2 parts probiotics and 0.5 parts fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

[0008] Furthermore, a compound animal milk formula containing natto probiotics includes the following components in parts by weight: 90 parts animal milk powder, 30 parts natto powder, 8 parts probiotics, and 2 parts fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

[0009] Furthermore, the animal milk powder is one or more of cow's milk, goat's milk, sheep's milk, or camel's milk powder.

[0010] Furthermore, the dietary nutrients include iron, zinc, calcium, selenium, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin D, and vitamin E; wherein the amount of vitamin B1 added is ≥0.5mg / 100g, and the amount of vitamin B2 added is ≥0.6mg / 100g.

[0011] Furthermore, the mass ratio of the animal milk powder to the dietary nutrients is (16~20):1.

[0012] Furthermore, the probiotic is one or two of Lactobacillus plantarum and Bifidobacterium, and the amount of probiotic added is 1×10⁻⁶. 8 CFU / g ~ 1×10 10 CFU / g, this type of probiotic is resistant to stomach acid and bile, and is easy to colonize in the intestines.

[0013] This invention also provides a method for preparing a compound animal milk formula containing natto probiotics, specifically including the following steps: (1) Fresh milk and dietary nutrients are mixed evenly and spray-dried to obtain animal milk powder compounded with dietary nutrients. (2) Dry mix the natto powder, probiotics, fructooligosaccharides and the animal milk powder with dietary nutrients prepared in step (1) and mix evenly to obtain a compound animal milk powder containing natto probiotics.

[0014] Furthermore, the inlet air temperature of the spray drying in step (1) is 160℃~180℃, and the outlet air temperature is 70℃~90℃.

[0015] Furthermore, the survival rate of probiotics in the product is no less than 85% in the acidic environment of the stomach, and there is no flocculation or stratification during the product's shelf life.

[0016] The technical solution provided by this invention has the following beneficial effects: (1) Adding fructooligosaccharides as a prebiotic, while utilizing its stable properties, improves the dispersibility of the milk base system, prevents probiotics from flocculating with milk protein, avoids protein denaturation and flavor deterioration during the shelf life of the product, and significantly improves product stability.

[0017] (2) Through the compound system of animal milk, natto powder and probiotics, the synergistic effect of nattokinase and probiotics is achieved. While regulating the balance of intestinal flora, nattokinase exerts fibrinolytic activity to help improve vascular health, thus achieving the dual targeted effects of intestinal conditioning and vascular maintenance. Detailed Implementation

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection.

[0019] This embodiment discloses the raw material ratio, preparation process, and performance test results of a compound animal milk formula containing natto probiotics.

[0020] The formulation of this invention, by weight, comprises 70-90 parts animal milk powder, 1-30 parts natto powder, 2-8 parts probiotics, and 0.5-2 parts fructooligosaccharides; the animal milk powder is compounded with dietary nutrients in a mass ratio of (16-20):1; the dietary nutrients include iron, zinc, calcium, selenium, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin D, and vitamin E; wherein, the amount of vitamin B1 added is ≥0.5mg / 100g, and the amount of vitamin B2 added is ≥0.6mg / 100g; the probiotics are one or two of Lactobacillus plantarum and Bifidobacterium, and the amount of live bacteria added is 1×10 8 CFU / g ~ 1×10 10 CFU / g.

[0021] The preparation method includes the following steps: (1) Mix fresh milk with dietary nutrients evenly, set the spray drying inlet temperature to 160℃~180℃ and the outlet temperature to 70℃~90℃, and obtain animal milk powder compounded with dietary nutrients after drying; (2) Dry mix natto powder, probiotics, fructooligosaccharides with the above animal milk powder, and obtain compound animal milk powder containing natto probiotics after mixing evenly.

[0022] Example 1: Compound Camel Milk Formula (a) Formulation components (by weight) Camel milk powder: 80 portions; Natto powder: 15.5 servings; Lactobacillus plantarum: 5 parts, live bacteria addition amount is 5×10 9 CFU / g; Fructooligosaccharides: 1.25 parts; Dietary nutrients: 4 servings, with a mass ratio of 20:1 to camel milk powder, including iron, zinc, calcium, selenium, vitamin A, vitamin B6, vitamin C, vitamin D, and vitamin E; of which vitamin B1 is added at ≥0.005 servings and vitamin B2 is added at ≥0.006 servings.

[0023] (II) Preparation method: (1) Mix fresh camel milk with dietary nutrients evenly, set the spray drying inlet air temperature to 170℃ and the outlet air temperature to 80℃, and obtain camel milk powder containing dietary nutrients after drying; (2) Dry mix natto powder, Lactobacillus plantarum, fructooligosaccharides and the above camel milk powder, the mixing rate is 150r / min and the mixing time is 20min to obtain mixed milk powder.

[0024] (III) Performance Test Results: Probiotic survival rate in the acidic environment of the stomach: 93%; Product shelf life: 24 months, no flocculation or stratification; Intestinal colonization ability: extremely strong.

[0025] Example 2: Compound Goat Milk Formula (a) Formulation components (by weight) Goat milk powder: 70 portions; Natto powder: 1 serving; Bifidobacterium: 2 portions, live bacteria addition amount is 1×10 8 CFU / g; Fructooligosaccharides: 0.5 parts; Dietary nutrients: 3.5 parts, with a mass ratio of 20:1 to goat milk powder, including iron, zinc, calcium, selenium, vitamin A, vitamin B6, vitamin C, vitamin D, and vitamin E; of which vitamin B1 is added at least 0.005 parts and vitamin B2 at least 0.006 parts.

[0026] (II) Preparation method: (1) Fresh goat milk is mixed with dietary nutrients evenly, and the spray drying inlet temperature is set to 160℃ and the outlet temperature is set to 70℃. After drying, goat milk powder containing dietary nutrients is obtained; (2) Natto powder, bifidobacteria, fructooligosaccharides and the above goat milk powder are dry mixed at a mixing rate of 120r / min and a mixing time of 15min to obtain mixed milk powder.

[0027] (III) Performance Test Results: Survival rate of probiotics in the acidic environment of the stomach: 86%; Product shelf life: 18 months, no flocculation or stratification; Intestinal colonization ability: strong.

[0028] Example 3: High-ratio compound sheep milk formula (a) Formulation components (by weight) Sheep milk powder: 90 portions; Natto powder: 30 servings; Lactobacillus plantarum + Bifidobacterium mixed bacteria: 8 portions, total live bacteria addition of 1×10⁻⁶ 10 CFU / g, the ratio of the two bacteria is 1:1; Fructooligosaccharides: 2 parts; Dietary nutrients: 5 servings, with a mass ratio of 18:1 to sheep milk powder, including iron, zinc, calcium, selenium, vitamin A, vitamin B6, vitamin C, vitamin D, and vitamin E; of which vitamin B1 is added at ≥0.005 servings and vitamin B2 is added at ≥0.006 servings.

[0029] (II) Preparation method: (1) Mix fresh sheep milk with dietary nutrients evenly, set the spray drying inlet air temperature to 180℃ and the outlet air temperature to 90℃, and obtain sheep milk powder containing dietary nutrients after drying; (2) Dry mix natto powder, mixed probiotics, fructooligosaccharides with the above sheep milk powder, mix at a mixing rate of 180r / min and a mixing time of 25min to obtain mixed milk powder.

[0030] (III) Performance Test Results: Probiotic survival rate in the acidic environment of the stomach: 96%; Product shelf life: 24 months, no flocculation or stratification; Intestinal colonization ability: extremely strong.

[0031] Example 4 Milk Formula (a) Formulation components (by weight) Milk powder: 80 portions; Natto powder: 15.5 servings; Lactobacillus plantarum: 5 parts, live bacteria addition amount is 8×10 9 CFU / g; Fructooligosaccharides: 1.25 parts; Dietary nutrients: 4 servings, with a mass ratio of 20:1 to the mixed milk powder, including iron, zinc, calcium, selenium, vitamin A, vitamin B6, vitamin C, vitamin D, and vitamin E; of which vitamin B1 is added at least 0.005 servings and vitamin B2 is added at least 0.006 servings.

[0032] The preparation method is the same as in Example 1; (II) Performance Test Results: Probiotic survival rate in the acidic environment of the stomach: 94%; Product shelf life: 24 months, no flocculation or stratification; Intestinal colonization ability: extremely strong.

[0033] The products prepared in the above embodiments meet the requirements for endurance products in GB24154 "National Food Safety Standard for Sports Nutrition Foods".

[0034] This invention significantly addresses the core technical challenges of existing milk-based probiotic products through the synergistic application of precise formulation and dry-mixing process control. The specific technical effects of the products in each embodiment are as follows: (1) Product stability is significantly enhanced The products in Examples 1, 3, and 4 have a shelf life of up to 24 months, while the product in Example 2 has a shelf life of up to 18 months. During the shelf life, there is no flocculation, layering, or flavor deterioration.

[0035] The addition of fructooligosaccharides improves the dispersibility of the milk-based system, effectively prevents the interaction between probiotics and milk proteins, and, combined with the dry-mixing process, avoids the damage of high temperatures to the components, significantly improving the product's storage stability.

[0036] (2) It has outstanding synergistic effects, achieving dual health benefits. All embodiments use a compound system of "animal milk + natto powder + specific probiotics", where the fibrinolytic activity of nattokinase and the intestinal regulatory effect of probiotics work synergistically.

[0037] The product combines the functions of regulating gut microbiota balance and improving vascular health, with its gut colonization ability reaching the level of "strong" to "extremely strong", which is superior to commercially available single probiotic milk powder products.

[0038] (3) The formula has strong adaptability and a wide range of applications. The examples cover a variety of bases such as camel milk, goat milk, sheep milk and mixed milk, and the formulations cover different gradients such as low ratio, preferred ratio and high ratio.

[0039] Different formulas can achieve stable technical effects, meet the needs of different consumer groups, and are feasible for large-scale industrial production and market promotion.

[0040] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.

[0041] The above description of the implementation methods is not restrictive, and the embodiments shown are only one implementation method; the actual structure is not limited to this. In short, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit, such designs should fall within the scope of protection.

Claims

1. A compound animal milk formula containing natto probiotics, characterized in that, It comprises the following components in parts by weight: 70-90 parts of animal milk powder, 1-30 parts of natto powder, 2-8 parts of probiotics and 0.5-2 parts of fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

2. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, It comprises the following components in parts by weight: 80 parts animal milk powder, 15.5 parts natto powder, 5 parts probiotics and 1.25 parts fructooligosaccharides; the animal milk powder is also formulated with dietary nutrients.

3. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, It comprises the following components in parts by weight: 70 parts animal milk powder, 1 part natto powder, 2 parts probiotics and 0.5 parts fructooligosaccharides; the animal milk powder is compounded with dietary nutrients.

4. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, It comprises the following components in parts by weight: 90 parts animal milk powder, 30 parts natto powder, 8 parts probiotics and 2 parts fructooligosaccharides; the animal milk powder is also formulated with dietary nutrients.

5. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, The animal milk powder is one or more of cow's milk, goat's milk, sheep's milk, or camel's milk powder.

6. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, The dietary nutrients include iron, zinc, calcium, selenium, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin D, and vitamin E; wherein the amount of vitamin B1 added is ≥0.5mg / 100g, and the amount of vitamin B2 added is ≥0.6mg / 100g.

7. The compound animal milk formula containing natto probiotics according to claim 6, characterized in that, The mass ratio of the animal milk powder to dietary nutrients is (16~20):

1.

8. The compound animal milk formula containing natto probiotics according to claim 1, characterized in that, The probiotics are one or two of Lactobacillus plantarum and Bifidobacterium, and the amount of probiotics added is 1×10⁻⁶. 8 CFU / g ~ 1×10 10 CFU / g.

9. The method for preparing the compound animal milk formula containing natto probiotics according to any one of claims 1 to 8, characterized in that, Specifically, the following steps are included: (1) Fresh milk and dietary nutrients are mixed evenly and spray-dried to obtain animal milk powder compounded with dietary nutrients. (2) Dry mix the natto powder, probiotics, fructooligosaccharides and the animal milk powder with dietary nutrients prepared in step (1) and mix evenly to obtain a compound animal milk powder containing natto probiotics.

10. The method for preparing the compound animal milk formula containing natto probiotics according to claim 1, characterized in that, The inlet air temperature of the spray drying in step (1) is 160℃~180℃, and the outlet air temperature is 70℃~90℃.