Fermentation device special for lactobacillus reuteri
By designing a fermentation device with a plug and a retention cylinder, the problem of contamination by miscellaneous bacteria during sampling from the fermenter was solved, aseptic sampling was achieved, and the fermentation effect was improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-13
- Publication Date
- 2026-04-07
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In existing fermenters, the culture medium is easily exposed to the external environment or the sampling tube during sampling, which can lead to the introduction of contaminating bacteria and affect the fermentation effect.
A dedicated fermentation device for Lactobacillus reuteri was designed. By using a stopper and a retention tube together, the culture medium can be sampled independently and in a closed manner, avoiding contact between the culture medium and the outside environment. A drive component is used to control the raising and lowering of the stopper to ensure the asepticity of the sampling process.
It effectively avoids contamination of the culture medium, improves fermentation results, and ensures the sterility and ease of operation of the fermentation process.
Smart Images
Figure CN121801671A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of fermentation equipment technology, and more specifically, to a fermentation equipment specifically for Lactobacillus reuteri. Background Technology
[0002] The core working principle of the Lactobacillus reuteri fermentation device is to simulate the optimal growth environment of Lactobacillus reuteri. By precisely controlling key parameters such as temperature, pH, oxygen, nutrient supply, and mixing efficiency, it achieves efficient proliferation and stable fermentation of the strain, while taking into account the monitorability, aseptic nature, and ease of operation of the fermentation process.
[0003] Lactobacillus reuteri is a facultative anaerobic bacterium that prefers low-oxygen or anaerobic environments. Its growth temperature range is approximately 35-39℃, with an optimal growth temperature of around 37℃. The suitable pH range is 5.5-6.5. It is sensitive to environmental fluctuations; therefore, the core of the device is "parameter closed-loop control." Temperature control mainly employs "jacketed temperature control" or "built-in heating / cooling components." Lactobacillus reuteri fermentation requires a low-oxygen or anaerobic environment. Before fermentation, the air inside the tank is replaced with inert gases such as nitrogen and carbon dioxide. During fermentation, the amount of oxygen introduced is adjusted according to the strain's requirements, or the system is completely sealed. Some devices are equipped with gas... The device includes a flow meter and oxygen concentration sensor to monitor the oxygen partial pressure inside the tank in real time. A built-in pH sensor detects the pH value of the culture medium in real time. A controller activates the alkali or acid pump to adjust the pH value inside the tank. Lactobacillus reuteri fermentation requires nutrients such as carbon, nitrogen, and vitamins. The tank-type device has a built-in agitator, and some units employ a "whole-tank rotation" design to ensure uniform distribution of the culture medium. Built-in sensors for temperature, pH, oxygen concentration, pressure, and liquid level transmit data to the controller in real time, allowing operators to visually view and monitor the fermentation status. Samples of the culture medium inside the tank also need to be taken.
[0004] Currently, existing fermenters typically have sampling ports on the side or bottom of the tank, equipped with valves and sampling tubes. Sampling involves first sterilizing the sampling port with steam or alcohol, then opening the valve to extract a quantitative amount of culture medium. After sampling, the valve is closed and sterilized again. However, whether the valve is open or the sampling tube is inserted into the valve to extract the culture medium, the culture medium inside the fermenter comes into direct contact with the external environment or the sampling tube. This makes it difficult to avoid contamination from external bacteria or residual bacteria on the sampling tube, which can pollute the culture medium and negatively impact fermentation efficiency. Therefore, this method does not meet current requirements. To address this, we propose a dedicated fermentation device for *Lactobacillus reuteri*. Summary of the Invention
[0005] This invention provides a dedicated fermentation device for Lactobacillus reuteri. This device can minimize the possibility of contamination of the culture medium in the fermenter with other bacteria during sampling, thus improving the fermentation effect. It solves the problem mentioned in the background art that the culture medium in the fermenter comes into direct contact with the external environment or the sampling tube during sampling, which can easily lead to contamination of the culture medium and affect the fermentation effect.
[0006] To achieve the above objectives, this disclosure provides a fermentation device specifically for Lactobacillus reuteri, comprising a fermenter, a connecting pipe disposed inside the fermenter, an insert pipe inserted into the side wall of the fermenter, and a sampling pipe. A plug is slidably disposed inside the connecting pipe, and a driving assembly is disposed outside the fermenter. The driving assembly is used to drive the plug to move up and down. A bent pipe is inserted inside the plug, and a fixing box is installed inside the insert pipe. A retention cylinder is installed inside the fixing box. An inlet pipe is inserted into the side wall of the fixing box and the retention cylinder. A first sealing plate is movably disposed at the end of the inlet pipe. The inlet pipe and the bent pipe are intermittently connected. An outlet pipe is also inserted into the side wall of the fixing box and the retention cylinder. A second sealing plate is movably disposed at the end of the outlet pipe. The outer wall of the sampling pipe slides in conjunction with the inner wall of the outlet pipe.
[0007] Optionally, the fermenter is provided with a culture medium, the lower end of the connecting pipe is inserted into the culture medium, and the driving assembly includes a box body installed on the top of the fermenter, a first chain slidably disposed inside the box body, a second chain slidably disposed inside the box body, a toothed groove formed on the side of the first chain, a gear rotatably disposed inside the box body and meshing with the toothed groove, and a motor installed on the outside of the box body for driving the gear.
[0008] Optionally, the first chain and the second chain are symmetrically arranged and mesh with each other. The lower ends of the first chain and the second chain are inserted into the top of the fermenter and the connecting pipe. The bottoms of the first chain and the second chain are connected to the plug. The output shaft of the motor is inserted into the side wall of the box and is connected to the gear.
[0009] Optionally, one end of the bend is located at the bottom of the plug body, and the other end of the bend is located at the side of the plug body. A sealing ring is connected to the opening of the bend located at the side of the plug body, and the inner wall of the connecting pipe is pressed against the end of the sealing ring.
[0010] Optionally, the outer wall of the fixing box is connected to the inner wall of the insertion tube, and the outer wall of the retention cylinder is connected to the inner wall of the fixing box.
[0011] Optionally, the first sealing sheet is slidably disposed on the outer wall of the fixed box, a first elastic telescopic rod is installed on the outer side of the first sealing sheet, the end of the first elastic telescopic rod is connected to the fixed box, and the plug intermittently abuts against the first sealing sheet.
[0012] Optionally, the second sealing strip is slidably disposed inside the retention cylinder, and a second elastic telescopic rod is installed on the outside of the second sealing strip. The end of the second elastic telescopic rod is connected to the retention cylinder, and the sampling tube intermittently contacts the second sealing strip.
[0013] Optionally, a piston is slidably disposed inside the retention cylinder, and a straight rod is mounted on the upper side of the piston. The middle part of the straight rod is inserted into the retention cylinder, the fixing box, and the side of the insertion tube. A sliding groove is provided on the inner wall of the fermenter, and a slider is slidably disposed in the sliding groove. A third elastic telescopic rod is disposed in the sliding groove, one end of the third elastic telescopic rod is connected to the slider, and the other end of the third elastic telescopic rod is connected to the inner wall of the fermenter.
[0014] Optionally, a shaft is rotatably inserted into the side of the slider, and a torsion spring is sleeved on the outside of the shaft. The two ends of the torsion spring are respectively connected to the shaft and the slider. A limit block is installed on the outside of the shaft, and a limit groove is opened on the inside of the slider to cooperate with the limit block. The limit block is slidably inserted into the limit groove. A lever is installed at the end of the shaft. One end of the lever is rotatably connected to the straight rod, and the other end of the lever intermittently abuts against the plug.
[0015] Optionally, a waste discharge pipe is inserted into the side wall of the fermenter, and a one-way valve is connected to the bottom of the retention cylinder. The waste discharge pipe is connected to the liquid outlet of the one-way valve, and the culture medium in the retention cylinder is discharged through the one-way valve and the waste discharge pipe.
[0016] With the above technical solution, the Lactobacillus reuteri fermentation device provided in this disclosure, when in use: by raising the stopper, the culture medium in the fermenter enters the retention cylinder through the connecting pipe; then the stopper moves down to reset, so that the culture medium in the fermenter and the culture medium in the retention cylinder are independently isolated, so that the culture medium in the retention cylinder cannot affect the culture medium in the fermenter, thus making the culture medium in the fermenter a completely independent and closed system, unaffected by external operations and contamination. Finally, the culture medium in the retention cylinder is extracted through the sampling tube to complete the sampling, thus minimizing the possibility of contamination of the culture medium in the fermenter during sampling, avoiding contamination of the culture medium in the fermenter, and thus improving the fermentation effect.
[0017] Other features and advantages of this disclosure will be described in detail in the following detailed description section. Attached Figure Description
[0018] The accompanying drawings are provided to further illustrate the present disclosure and form part of the specification. They are used together with the following detailed description to explain the present disclosure, but do not constitute a limitation thereof. In the drawings: Figure 1 This is a three-dimensional structural diagram of the present invention.
[0019] Figure 2 This is a schematic diagram of the first state cross-section structure of the present invention.
[0020] Figure 3 For the present invention Figure 2 Enlarged structural diagram at point A in the middle.
[0021] Figure 4 This is a schematic diagram of the second state cross-sectional structure of the present invention.
[0022] Figure 5 For the present invention Figure 4 Enlarged structural diagram at point B.
[0023] Figure 6 This is a partial three-dimensional structural diagram of the present invention.
[0024] Figure 7 This is a schematic diagram of the structure where the plug and the retention cylinder are far apart according to the present invention.
[0025] Figure 8 This is a schematic diagram of the close proximity structure of the plug and the retention cylinder of the present invention.
[0026] Figure 9 This is a partial exploded view of the present invention.
[0027] Explanation of reference numerals in the attached drawings: 100, fermenter; 110, connecting pipe; 120, insertion tube; 130, sampling tube; 140, plug; 150, drive assembly; 151, box body; 152, first chain; 153, second chain; 154, toothed groove; 155, gear; 156, motor; 160, bend; 161, sealing ring; 170, fixing box; 180, retention cylinder; 190, liquid inlet pipe; 200 201. First sealing plate; 210. First elastic telescopic rod; 220. Liquid outlet pipe; 221. Second sealing plate; 222. Second elastic telescopic rod; 230. Piston; 231. Straight rod; 232. Slide groove; 233. Slider; 234. Third elastic telescopic rod; 240. Shaft; 241. Torsion spring; 242. Limiting block; 243. Limiting groove; 244. Lever; 250. Waste discharge pipe; 251. One-way valve. Detailed Implementation
[0028] To make the above-described objects, features, and advantages of this disclosure more apparent and understandable, specific embodiments of this disclosure will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this disclosure. However, this disclosure can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this disclosure. Therefore, this disclosure is not limited to the specific embodiments disclosed below.
[0029] In the description of this disclosure, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are used only for the convenience of describing this disclosure and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this disclosure. The terms "first" and "second" are used to distinguish one element from another and do not have sequential or importance. Furthermore, in the following description, when referring to the accompanying drawings, the same reference numerals in different drawings denote the same or similar elements, which will not be repeated here.
[0030] In this disclosure, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this disclosure according to the specific circumstances.
[0031] According to some embodiments of this disclosure, a fermentation apparatus specifically for Lactobacillus reuteri is provided, see reference. Figure 1 — Figure 9 As shown, the Lactobacillus reuteri fermentation device includes a fermenter 100, a connecting pipe 110 fixedly installed inside the fermenter 100, an insertion pipe 120 interference-fitted into the side wall of the fermenter 100, and a sterile sampling pipe 130 for aspirating the culture medium. The fermenter 100 contains a culture medium mixed with Lactobacillus reuteri. The lower end of the connecting pipe 110 is inserted into the culture medium. A plug 140 is slidably installed inside the connecting pipe 110. A drive assembly 150 is installed outside the fermenter 100. The drive assembly 150 is used to drive the plug 140 to rise and fall.
[0032] The drive assembly 150 includes a box 151 fixedly mounted on the top of the fermenter 100, a first chain 152 slidably mounted inside the box 151, a second chain 153 slidably mounted inside the box 151, a toothed groove 154 formed on the side of the first chain 152, a gear 155 rotatably mounted inside the box 151 and meshing with the toothed groove 154, and a motor 156 fixedly mounted on the outside of the box 151 for driving the gear 155. The first chain 152 and the second chain 153 are symmetrically arranged and mesh with each other. The lower ends of the first chain 152 and the second chain 153 are inserted into the top of the fermenter 100 and the connecting pipe 110. The bottoms of the first chain 152 and the second chain 153 are fixedly connected to the top of the plug 140. The output shaft of the motor 156 is inserted into the side wall of the box 151 and is fixedly connected to the gear 155.
[0033] A bent tube 160 is fixedly inserted inside the plug body 140. One end of the bent tube 160 is located at the bottom of the plug body 140, and the other end of the bent tube 160 is located at the side of the plug body 140. A sealing ring 161 is fixedly connected to the opening of the bent tube 160 located at the side of the plug body 140. The sealing ring 161 is an elastic rubber ring. The inner wall of the connecting tube 110 is pressed against the end of the sealing ring 161. The outer wall of the plug body 140 slides against the inner wall of the connecting tube 110. A fixing box 170 is fixedly installed inside the insertion tube 120. A retention cylinder 180 is fixedly installed inside the fixing box 170. The outer wall of the fixing box 170 is fixedly connected to the inner wall of the insertion tube 120, and the outer wall of the retention cylinder 180 is fixedly connected to the inner wall of the fixing box 170.
[0034] A liquid inlet pipe 190 is inserted into the side wall of the fixed box 170 and the retention cylinder 180. A first sealing plate 200 is movably provided at the end of the liquid inlet pipe 190. The liquid inlet pipe 190 is intermittently connected to the bend pipe 160. The first sealing plate 200 is slidably provided on the outer wall of the fixed box 170. A first elastic telescopic rod 201 is fixedly installed on the outside of the first sealing plate 201. The end of the first elastic telescopic rod 201 is fixedly connected to the fixed box 170. The plug body 140 intermittently abuts against the first sealing plate 200. A liquid outlet pipe 210 is also inserted into the side wall of the fixed box 170 and the retention cylinder 180. A second sealing plate 220 is movably provided at the end of the liquid outlet pipe 210. The outer wall of the sampling tube 130 slides in cooperation with the inner wall of the liquid outlet pipe 210.
[0035] The second sealing plate 220 is slidably disposed inside the retention cylinder 180. A second elastic telescopic rod 221 is fixedly installed on the outside of the second sealing plate 220. The end of the second elastic telescopic rod 221 is fixedly connected to the retention cylinder 180. A groove is opened in the inner wall of the retention cylinder 180. The second sealing plate 220 is slidably inserted into the groove. An elastic sealing membrane (not shown in the figure) is connected between the second sealing plate 220 and the groove. The elastic sealing membrane seals the groove opening of the groove without hindering the normal sliding of the second sealing plate 220, thus preventing the culture medium in the retention cylinder 180 from flowing into the groove and being difficult to drain. The elastic sealing membrane sealing the groove opening is a technical means well known to those skilled in the art and will not be described in detail here.
[0036] The sampling tube 130 intermittently contacts the second sealing plate 220. The end of the sampling tube 130 can be connected to the vacuum sampling cylinder. When the sampling tube 130 is inserted into the retention cylinder 180, the suction in the vacuum sampling cylinder can draw the culture medium in the retention cylinder 180 into the vacuum sampling cylinder, thereby completing the sampling. The sampling hole of the sampling tube 130 is opened on the side wall of the sampling tube 130, thereby avoiding the situation where the end of the sampling tube 130 is covered by the second sealing plate 220 when the sampling tube 130 contacts the second sealing plate 220, making it difficult to extract liquid. The end of the liquid outlet pipe 210 located outside the fermenter 100 is provided with threads and a pipe cap that is threadedly matched with it. When the liquid outlet pipe 210 is not in use, the pipe cap can be threadedly connected to the end of the liquid outlet pipe 210 to seal the liquid outlet pipe 210, thereby minimizing the influence of the external environment on the liquid outlet pipe 210.
[0037] A piston 230 is slidably installed inside the retention cylinder 180. A straight rod 231 is fixedly installed on the upper side of the piston 230. The middle part of the straight rod 231 is inserted into the side of the retention cylinder 180, the fixing box 170, and the insertion tube 120. A groove 232 is opened on the inner wall of the fermentation tank 100. A slider 233 is slidably installed in the groove 232. A third elastic telescopic rod 234 is installed in the groove 232. One end of the third elastic telescopic rod 234 is fixedly connected to the slider 233, and the other end of the third elastic telescopic rod 234 is fixedly connected to the inner wall of the fermentation tank 100.
[0038] A shaft 240 is rotatably inserted into the side of slider 233. A torsion spring 241 is sleeved on the outside of shaft 240. Both ends of torsion spring 241 are fixedly connected to shaft 240 and slider 233 respectively. A limit block 242 is fixedly installed on the outside of shaft 240. A limit groove 243 is formed on the inside of slider 233 to cooperate with limit block 242. The limit groove 243 is set as an arc groove. Limit block 242 is slidably inserted into limit groove 243. A lever 244 is fixedly installed at the end of shaft 240. One end of lever 244 is rotatably connected to straight rod 231, and the other end of lever 244 intermittently contacts plug 140. A waste discharge pipe 250 is interference-fitted into the side wall of fermenter 100. A one-way valve 251 is connected to the bottom of retention cylinder 180. The waste discharge pipe 250 is connected to the liquid outlet of one-way valve 251. The culture medium in retention cylinder 180 is discharged through one-way valve 251 and waste discharge pipe 250. In the initial state, plug 140 is located at the lower end of connecting pipe 110, which closes the connecting pipe 110.
[0039] With the above technical solution, the Lactobacillus reuteri fermentation device provided in this disclosure firstly drives the gear 155 to rotate through the motor 156. The meshing of the gear 155 and the tooth groove 154 causes the lower end of the first chain 152 to rise. At the same time, the meshing of the first chain 152 and the second chain 153 causes the lower end of the second chain 153 to rise synchronously with the first chain 152, thereby driving the plug 140 to rise along the inner wall of the connecting pipe 110, thereby drawing the culture medium in the fermenter 100 into the connecting pipe. As the plug 140 rises, its top pushes one end of the lever 244 upwards, causing the shaft 240 to slide the limiting block 242 within the limiting groove 243 and compressing the torsion spring 241. Simultaneously, the other end of the lever 244 pushes the straight rod 231 and piston 230 downwards, discharging residual gas or culture medium in the retention cylinder 180 through the one-way valve 251 and the waste discharge pipe 250 to the outside of the fermenter 100, thus emptying the retention cylinder 180. When the piston 230 is pressed down to the bottom of the retention cylinder 180, the limiting block 242 moves to the end of the limiting groove 243, so that the shaft 240 can no longer rotate. At this time, the rising plug 140 drives the lever 244, slider 233, straight rod 231 and piston 230 to move upward, so that the slider 233 slides in the groove 232 to compress the third elastic telescopic rod 234. And because the piston 230 moves upward, the retention cylinder 180 remains sealed, thus creating a negative pressure state inside the retention cylinder 180. Furthermore, the rising plug 140 pushes the first sealing plate 200 upward and compresses the first elastic telescopic rod 201. When the first sealing plate 200 moves upward and releases the seal on the inlet pipe 190, the motor 156 stops, causing the plug 140 to stop rising and remain stationary. At this time, the bent pipe 160 connects with the inlet pipe 190. The sealing ring 161 increases the sealing performance of the connection between the bent pipe 160 and the inlet pipe 190, allowing the culture medium in the connecting pipe 110 to flow negatively within the retention cylinder 180. Under the suction force, the liquid is drawn into the retention cylinder 180 through the bend 160 and the inlet pipe 190. The output shaft of the motor 156 is restarted and rotated in the opposite direction, so that the plug 140 is pushed down by the first chain 152 and the second chain 153 and reset. The bend 160 is disengaged from the inlet pipe 190, the first sealing plate 200 re-closes the inlet pipe 190, and the downward-moving plug 140 pushes the culture medium in the connecting pipe 110 back into the fermenter 100, so that the inside of the connecting pipe 110 is kept sealed and clean again. Finally, the sterile sampling tube 130 is inserted into the outlet tube 210, causing the end of the sampling tube 130 to push open the second sealing plate 220, allowing the end of the sampling tube 130 to be inserted into the culture medium in the retention cylinder 180, thus completing the sampling. In summary, the plug body 140 first rises, allowing the culture medium in the fermenter 100 to enter the retention cylinder 180 through the connecting tube 110. Then, the plug body 140 moves down to reset, independently isolating the culture medium in the fermenter 100 from the culture medium in the retention cylinder 180. This prevents the culture medium in the retention cylinder 180 from affecting the culture medium in the fermenter 100, thus restoring the culture medium in the fermenter 100 to a completely independent and closed system, unaffected by external operations or contamination. Finally, the sampling tube 130 is used to extract the culture medium in the retention cylinder 180, thus completing the sampling. This process minimizes the possibility of contamination of the culture medium in the fermenter during sampling, preventing contamination and improving the fermentation effect.
[0040] It should be noted that after sampling, lever 244 and piston 230 are reset under the rebound force of the third elastic telescopic rod 234 and torsion spring 241, and second sealing plate 220 is reset under the rebound force of the second elastic telescopic rod 221. During the second sampling, the same principle applies. The plug 140 rises again, and the upward action of the plug 140 on one end of lever 244 causes the other end of lever 244 to press down and then lift piston 230 through straight rod 231. This allows the culture medium remaining in retention cylinder 180 during the first sampling to be cleaned through one-way valve 251 and waste discharge pipe 250. The culture medium from the first sampling is discharged to facilitate the second sampling. However, it is unavoidable that some culture medium from the first sampling will remain on the inner wall of the connecting tube 110 or the retention cylinder 180, which can easily mix into the culture medium in the second sampling, thus affecting the accuracy of the second sampling. Therefore, the culture medium from the second sampling can be treated as a waste sample, and then a third sampling can be performed. Since the second sampling operation has served to rinse the inner wall of the connecting tube 110 and the retention cylinder 180, it effectively avoids the situation of culture medium residue from the first sampling. Therefore, the third sampling can obtain an accurate sample.
[0041] The preferred embodiments of this disclosure have been described in detail above with reference to the accompanying drawings. However, this disclosure is not limited to the specific details of the above embodiments. Within the scope of the technical concept of this disclosure, various simple modifications can be made to the technical solutions of this disclosure, and these simple modifications all fall within the protection scope of this disclosure.
[0042] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, this disclosure will not describe the various possible combinations separately.
[0043] Furthermore, various different embodiments of this disclosure can be combined in any way, as long as they do not violate the spirit of this disclosure, they should also be regarded as the content disclosed in this disclosure.
Claims
1. A fermentation device specifically for Lactobacillus reuteri, comprising a fermenter (100), a connecting pipe (110) disposed inside the fermenter (100), an insertion pipe (120) inserted into the side wall of the fermenter (100), and a sampling pipe (130), characterized in that: A plug (140) is slidably disposed inside the connecting pipe (110). A driving assembly (150) is disposed outside the fermenter (100). The driving assembly (150) is used to drive the plug (140) to rise and fall. A bent pipe (160) is inserted inside the plug (140). A fixing box (170) is installed inside the inserted pipe (120). A retention cylinder (180) is installed inside the fixing box (170). The fixing box (170) and the retention cylinder (180) are connected. The side walls of the fixed box (170) and the retention cylinder (180) are connected together by an inlet pipe (190), and a first sealing plate (200) is movably provided at the end of the inlet pipe (190). The inlet pipe (190) and the bend pipe (160) are intermittently connected. The side walls of the fixed box (170) and the retention cylinder (180) are also connected together by an outlet pipe (210), and a second sealing plate (220) is movably provided at the end of the outlet pipe (210). The outer wall of the sampling tube (130) and the inner wall of the outlet pipe (210) are slidably fitted.
2. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: The fermenter (100) is filled with a culture medium, and the lower end of the connecting pipe (110) is inserted into the culture medium. The drive assembly (150) includes a box (151) installed on the top of the fermenter (100), a first chain (152) slidably disposed inside the box (151), a second chain (153) slidably disposed inside the box (151), a toothed groove (154) opened on the side of the first chain (152), a gear (155) rotatably disposed inside the box (151) and meshing with the toothed groove (154), and a motor (156) installed outside the box (151) for driving the gear (155).
3. The fermentation apparatus for Lactobacillus reuteri according to claim 2, characterized in that: The first chain (152) and the second chain (153) are symmetrically arranged and mesh with each other. The lower ends of the first chain (152) and the second chain (153) are inserted into the top of the fermenter (100) and the connecting pipe (110). The bottoms of the first chain (152) and the second chain (153) are connected to the plug (140). The output shaft of the motor (156) is inserted into the side wall of the box (151) and the output shaft of the motor (156) is connected to the gear (155).
4. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: One end of the bent tube (160) is located at the bottom of the plug body (140), and the other end of the bent tube (160) is located on the side of the plug body (140). A sealing ring (161) is connected to the opening of the bent tube (160) located on the side of the plug body (140), and the inner wall of the connecting tube (110) is pressed against the end of the sealing ring (161).
5. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: The outer wall of the fixing box (170) is connected to the inner wall of the insertion tube (120), and the outer wall of the retention tube (180) is connected to the inner wall of the fixing box (170).
6. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: The first sealing sheet (200) is slidably disposed on the outer wall of the fixed box (170). A first elastic telescopic rod (201) is installed on the outer side of the first sealing sheet (200). The end of the first elastic telescopic rod (201) is connected to the fixed box (170). The plug (140) intermittently abuts against the first sealing sheet (200).
7. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: The second sealing plate (220) is slidably disposed inside the retention cylinder (180), and a second elastic telescopic rod (221) is installed on the outside of the second sealing plate (220). The end of the second elastic telescopic rod (221) is connected to the retention cylinder (180), and the sampling tube (130) intermittently contacts the second sealing plate (220).
8. The fermentation apparatus for Lactobacillus reuteri according to claim 1, characterized in that: A piston (230) is slidably disposed inside the retention cylinder (180). A straight rod (231) is installed on the upper side of the piston (230). The middle part of the straight rod (231) is inserted into the side of the retention cylinder (180), the fixing box (170), and the insertion tube (120). A sliding groove (232) is opened on the inner wall of the fermentation tank (100). A slider (233) is slidably disposed in the sliding groove (232). A third elastic telescopic rod (234) is disposed in the sliding groove (232). One end of the third elastic telescopic rod (234) is connected to the slider (233), and the other end of the third elastic telescopic rod (234) is connected to the inner wall of the fermentation tank (100).
9. The fermentation apparatus for Lactobacillus reuteri according to claim 8, characterized in that: A shaft (240) is rotatably inserted into the side of the slider (233). A torsion spring (241) is sleeved on the outside of the shaft (240). The two ends of the torsion spring (241) are connected to the shaft (240) and the slider (233) respectively. A limit block (242) is installed on the outside of the shaft (240). A limit groove (243) is opened on the inside of the slider (233) to cooperate with the limit block (242). The limit block (242) is slidably inserted into the limit groove (243). A lever (244) is installed at the end of the shaft (240). One end of the lever (244) is rotatably connected to the straight rod (231), and the other end of the lever (244) intermittently abuts against the plug (140).
10. A fermentation apparatus for Lactobacillus reuteri according to claim 9, characterized in that: The fermenter (100) is connected to a waste discharge pipe (250) on its side wall, and a one-way valve (251) is connected to the bottom of the retention cylinder (180). The waste discharge pipe (250) is connected to the liquid outlet of the one-way valve (251), and the culture medium in the retention cylinder (180) is discharged through the one-way valve (251) and the waste discharge pipe (250).