Environment-friendly spray for inhibiting incubation of ampullaria gigas eggs and use method of environment-friendly spray

Abstract

The invention discloses an environment-friendly spray for inhibiting incubation of ampullaria gigas eggs and a use method of the environment-friendly spray. The environment-friendly spray is characterized by comprising chitosan, acetic acid, sodium chloride, tea saponin, an organic silicon additive and a solvent; comprising the following steps: step 1, spraying opportunity; step 2, spraying preparation; step 3, a spraying mode; and step 4, effect verification. The environment-friendly spray takes natural active ingredients as a core, is environment-friendly and residue-free, is friendly to non-target organisms, can accurately inhibit incubation of ampullaria gigas eggs and block expansion of ampullaria gigas population from the source, and is simple in preparation process, convenient to use and suitable for large-area popularization and application.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological control, in particular to an environment-friendly spray for inhibiting the hatching of Pomacea canaliculata and a use method thereof. BACKGROUND

[0002] Pomacea canaliculata, also known as snow snail, apple snail, gold snail, and large bottle snail, belongs to the Gastropoda class of the Mollusca phylum, and is originally from the Amazon River Basin in South America. In the 1980s, Pomacea canaliculata was introduced into China, with the core purpose of promoting the breeding of edible snails. However, due to its coarse meat quality and poor taste, and the fact that it easily carries parasites such as the Guangzhou tube roundworm in its body, eating uncooked Pomacea canaliculata poses a high health risk, resulting in low market acceptance and poor breeding efficiency, and thus a large number of abandoned breeding and human discarding. Due to its strong adaptability, fast reproduction speed, and wide food range, Pomacea canaliculata has proliferated in large quantities in rice fields, ditches, lakes, and other water bodies in many provinces in southern China. Pomacea canaliculata has a wide food range, not only preying on the seedlings and tender stems of various crops such as rice, lotus root, and water chestnut, causing serious agricultural economic losses, but also disrupting the ecological balance of water bodies and preying on the eggs and larvae of local aquatic organisms, leading to a decline in local species diversity. Today, Pomacea canaliculata has become a key invasive aquatic organism for prevention and control in China.

[0003] Currently, the prevention and control methods for Pomacea canaliculata mainly include physical control, chemical control, and biological control. Physical control, such as manual capture of adult snails and removal of egg masses, is environmentally friendly but inefficient, making it difficult to meet the prevention and control needs of large areas of farmland or water bodies; chemical control often uses chemical agents such as niclosamide and sodium pentachlorophenol, which can quickly kill adult snails and eggs, but have strong toxicity to beneficial organisms such as fish, shrimp, and shellfish in water bodies, easily causing water pollution, and have a long residual period, which can damage the ecological environment and also may lead to the development of drug resistance in Pomacea canaliculata; biological control, such as using natural enemies such as ducks and geese for predation, or releasing parasitic wasps and pathogenic microorganisms, is environmentally friendly but is greatly restricted by environmental conditions and regions, has unstable control effects, and is difficult to achieve precise and efficient control.

[0004] Due to the strong reproductive capacity of Pomacea canaliculata, one female snail can reproduce 2-3 generations per year, with each laying several hundred eggs, and the eggs are attached to the surface of objects such as rice stems, field ridge weeds, and river stones, with a tough protective layer of gelatinous film, and have a high hatching rate, which is one of the key reasons why it is difficult to eradicate. Therefore, if the hatching of Pomacea canaliculata eggs can be inhibited, the population reproduction can be blocked from the source, which would be an efficient and environmentally friendly control idea. In the prior art, there are few control methods for Pomacea canaliculata eggs, and most of them rely on chemical agents, which have the problems of poor environmental friendliness and low safety. Therefore, it is of great practical significance and application value to develop an environmentally friendly, efficient, and ecologically friendly spray for inhibiting the hatching of Pomacea canaliculata eggs. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application provides an environmentally friendly spray for inhibiting the hatching of golden apple snail eggs and a use method thereof, which takes natural active ingredients as the core, is environmentally friendly and residue-free, friendly to non-target organisms, can accurately inhibit the hatching of golden apple snail eggs, block the expansion of golden apple snail population from the source, and is simple in preparation process and convenient to use, suitable for large-area popularization and application.

[0006] To achieve the above-mentioned object, the present application provides the following technical scheme: an environmentally friendly spray for inhibiting the hatching of golden apple snail eggs, comprising an environmentally friendly spray, wherein the environmentally friendly spray comprises chitosan, acetic acid, sodium chloride, tea saponin, an organic silicon additive, and a solvent.

[0007] The present application is further provided that: when the environmentally friendly spray is prepared in an amount of 10000ml, 50g of chitosan, 150ml of acetic acid, 50g of sodium chloride, 50g of tea saponin, and 10-20ml of the organic silicon additive are selected for preparation, and an appropriate amount of the solvent is selected, and the solvent is added to make the total amount of the liquid reach 10000ml.

[0008] The present application is further provided that: the preparation of the environmentally friendly spray comprises the following steps: Step 1: a container A is taken and 2000ml of pure water is stored in the container A, the temperature of the solvent is controlled at 40-50℃, then 150ml of 5% acetic acid is added to the container A and stirred uniformly to obtain a mixed solution I; Step 2: 50g of chitosan is slowly added to the solution I in the container A, the chitosan is water-soluble chitosan, since the acetic acid added in step 1 can provide a weak acid environment to promote the dissolution of chitosan, the stirring is continuously carried out at a speed of 300-500r / min for 30min until the chitosan is completely dissolved to obtain a mixed solution II, at this time, the solution II is transparent and slightly sticky; Step 3: a container B is taken and 3000ml of pure water is stored in the container B, then 50g of sodium chloride is added to the container B and stirred uniformly to obtain a mixed solution III, the addition of sodium chloride can increase the osmotic pressure to destroy the embryonic cells in the golden apple snail eggs, resulting in the death of the eggs due to dehydration; Step 4: 50g of tea saponin is added to the solution III in the container B and stirred uniformly to obtain a mixed solution IV, the tea saponin can dissolve the mucilage layer on the surface of the egg mass when sprayed on the surface of the golden apple snail eggs, thereby enhancing the permeability of the environmentally friendly spray; Step 5: an organic silicon additive is further added to the solution IV in the container B and stirred uniformly to obtain a mixed solution V; the organic silicon additive can be selected in an amount of 10-20ml, and the organic silicon additive can reduce the surface tension to make the environmentally friendly spray uniformly adhere to the gaps on the surface of the golden apple snail eggs; Step 6, slowly pour the solution II obtained in step 2 into the solution V obtained in step 5, and stir evenly while pouring, to obtain a mixed solution VI; Step 7, add an appropriate amount of pure water to the solution VI obtained in step 6 and stir evenly, so that the total amount of the mixed liquid reaches 10000ml, and stand for 10min to obtain the environment-friendly spray for inhibiting the hatching of apple snail eggs.

[0009] On the other hand, in order to achieve the above-mentioned purpose, the present application provides the following technical scheme: a use method of the environment-friendly spray for inhibiting the hatching of apple snail eggs, comprising spraying the surface of the apple snail with the environment-friendly spray for inhibiting the hatching of apple snail eggs.

[0010] The present application is further provided: comprising the following steps: Step 1, spraying timing: the environment-friendly spray is sprayed when the apple snail eggs are bright red, i.e. 5-7 days before the hatching of the apple snail eggs, and the spraying time needs to be selected at noon; Step 2, spraying preparation: before the spraying work is carried out, the staff needs to wear a mask, gloves and goggles, slowly pour the prepared environment-friendly spray into the sprayer, and stand for at least 10min; Step 3, spraying method: spray the surface of the apple snail eggs through the sprayer until the surface of the apple snail eggs is wet and shiny, and the spraying distance between the nozzle of the sprayer and the apple snail eggs is controlled at 10-15cm; Step 4, effect verification: if the color of the apple snail eggs changes to bright red and the surface film is invaded within 24h after spraying, the spraying is effective, and the mortality rate of the apple snail eggs is not less than 80%, and after 72h of spraying, the eggshell of the apple snail is hardened, the film is dried and condensed, and the color of the eggs is pink; if the surface film of the apple snail eggs does not change color within 72h after spraying, the surface has not been invaded, the spraying is ineffective, and step 3 needs to be repeated to spray the apple snail again.

[0011] The present application is further provided: comprising the following steps: Step 1, spraying timing: the environment-friendly spray is sprayed when the apple snail eggs are bright red, i.e. 5-7 days before the hatching of the apple snail eggs, and the spraying time needs to be selected at noon; Step 2, spraying preparation: before the spraying work is carried out, the staff needs to wear a mask, gloves and goggles, slowly pour the prepared environment-friendly spray into the sprayer, and stand for at least 10min; Step 3, preliminary treatment: the surface of the apple snail eggs is cleaned with a brush; Step 4, spraying method: spray the surface of the apple snail eggs through the sprayer until the surface of the apple snail eggs is wet and shiny, and the spraying distance between the nozzle of the sprayer and the apple snail eggs is controlled at 10-15cm; Step 5, Effect Verification: If the golden apple snail eggs turn bright red within 24 hours of spraying and the surface membrane is penetrated, the spraying is effective. The mortality rate of the golden apple snail eggs is not less than 80%. After 72 hours of spraying, the eggshells of the golden apple snails harden, the membrane dries and solidifies, and the egg color turns pink. If the surface membrane of the golden apple snail eggs does not change color within 72 hours of spraying, the surface has not been penetrated, the spraying is ineffective, and step 4 needs to be repeated to spray the golden apple snails again.

[0012] The present invention is further configured such that: before re-spraying the golden apple snail eggs, the pH of the environmentally friendly spray is tested. If the pH test strip shows a pH value of 5-6, the environmentally friendly spray is in a normal state and can continue to be used; otherwise, it cannot be used and needs to be re-prepared.

[0013] The present invention is further configured such that the environmentally friendly spray is used no more than 3 times per quarter.

[0014] The present invention is further configured such that: the environmentally friendly spray must not be poured directly into fish ponds or rivers after use, and must be disposed of in a harmless manner.

[0015] In summary, the present invention has the following beneficial effects: The chitosan in the environmentally friendly spray forms a film on the surface of the golden apple snail eggs, preventing them from respiring and causing them to suffocate and die, thus achieving the killing effect. At the same time, the sodium chloride in the environmentally friendly spray increases the osmotic pressure of the golden apple snail eggs, causing them to dehydrate and die, also achieving the killing effect. In addition, the tea saponin in the environmentally friendly spray dissolves the mucus layer on the surface of the golden apple snail eggs and has a certain degree of toxicity, causing them to die, thus achieving the killing effect. Because the above three methods of killing golden apple snails work together, the effectiveness of the environmentally friendly spray in killing golden apple snail eggs is effectively guaranteed. Detailed Implementation

[0016] The present invention will now be described in further detail.

[0017] Example 1: An environmentally friendly spray for inhibiting the hatching of golden apple snail eggs, comprising chitosan, acetic acid, sodium chloride, tea saponin, organosilicon additives, and solvents.

[0018] When preparing a solution for 10,000 ml, use 50 g chitosan, 150 ml acetic acid, 50 g sodium chloride, 50 g tea saponin, and 10-20 ml organosilicon additive. Select an appropriate amount of solvent to make the total liquid volume reach 10,000 ml.

[0019] The chitosan used is water-soluble. After dissolving, the water-soluble chitosan forms a viscous film that blocks oxygen exchange in the golden apple snail eggs. 5% acetic acid provides a weakly acidic environment, making the pH of the environmentally friendly spray approximately 5, promoting chitosan dissolution and softening the calcareous layer of the golden apple snail egg shell. Sodium chloride can destroy the embryonic cells inside the golden apple snail eggs through osmotic pressure, leading to dehydration and death. Tea saponin can dissolve the mucus layer on the surface of the golden apple snail eggs, enhancing the permeability of the environmentally friendly spray. Furthermore, tea saponin is harmless to shrimp but has low toxicity / tolerance effects on fish, making it safe to use in rice-shrimp farming and allowing for increased dosages to eliminate unwanted fish in rice paddies. Organosilicon additives reduce surface tension, ensuring the environmentally friendly spray adheres evenly to the gaps in the egg mass. Purified water reduces impurities in the environmentally friendly spray, improving its effectiveness.

[0020] Because the chitosan in the environmentally friendly spray forms a film on the surface of the golden apple snail eggs, it prevents the snails from respiring and causes them to suffocate and die, thus achieving the killing effect. At the same time, the sodium chloride in the environmentally friendly spray increases the osmotic pressure of the golden apple snail eggs, causing them to dehydrate and die, also achieving the killing effect. In addition, the tea saponin in the environmentally friendly spray dissolves the mucus layer on the surface of the golden apple snail eggs and has a certain degree of toxicity, causing them to die, thus achieving the killing effect. Due to the simultaneous action of the triple killing effect, the effectiveness of the environmentally friendly spray in killing golden apple snail eggs is guaranteed.

[0021] The preparation of the environmentally friendly spray includes the following steps: Step 1: Take container A and put 2000ml of purified water in container A. The temperature of the solvent is controlled at 40-50℃. Then, select 5% acetic acid and add 150ml of 5% acetic acid to container A and stir evenly to obtain mixed solution I. Step 2: Slowly add 50g of chitosan to solution I in container A. Water-soluble chitosan is selected. Since the acetic acid added in step 1 can provide a weak acid environment to promote the dissolution of chitosan, stir continuously at a speed of 300-500r / min for 30min until the chitosan is completely dissolved to obtain mixed solution II. At this time, solution II is transparent and slightly viscous. Step 3: Take container B and put 3000ml of purified water in container B. Then add 50g of sodium chloride to bottle B and stir well to obtain mixed solution III. Adding sodium chloride can increase the osmotic pressure and destroy the embryonic cells in the golden apple snail egg, causing it to dehydrate and die. Step 4: Add 50g of tea saponin to solution III in container B and stir well to obtain mixed solution IV. When tea saponin is sprayed on the surface of golden apple snail eggs, it can dissolve the mucus layer on the surface of the egg mass and enhance the penetration of the environmentally friendly spray. Step 5: Add silicone additive to solution IV in container B and stir evenly to obtain mixed solution V; 10-20 ml of silicone additive can be used. Silicone additive can reduce surface tension and make the environmentally friendly spray evenly adhere to the crevices on the surface of golden apple snail eggs. Step 6: Slowly pour the solution II obtained in step 2 into the solution V obtained in step 5, stirring evenly while pouring, to obtain a mixed solution VI; Step 7: Add an appropriate amount of purified water to solution VI obtained in step 6 and stir well so that the total volume of the mixed liquid reaches 10000ml. Let it stand for 10 minutes to obtain an environmentally friendly spray that inhibits the hatching of golden apple snail eggs.

[0022] Example 2, a method for controlling golden apple snail eggs, includes the following steps: Step 1, Spraying time: Spray with an environmentally friendly spray for golden apple snail eggs when the eggs are bright red, that is, 5-7 days before the golden apple snail eggs hatch. At the same time, the spraying time should be chosen at noon. During the day, the strong light will easily affect the evaporation and the spraying effect, while at night, the light is weak and it is not conducive to the work. Step 2, Spraying Preparation: Before spraying, staff must wear masks, gloves and goggles. Tea saponins and acetic acid in the environmentally friendly spray can easily enter the respiratory tract or adhere to the skin surface during spraying, thus causing irritation. Therefore, protective measures are necessary. Slowly pour the prepared golden apple snail eggs into the sprayer using the environmentally friendly spray and let it stand for at least 10 minutes. Letting it stand can prevent foam from being generated during the pouring process, which would affect the spraying effect. Step 3, Spraying method: Spray the solution onto the surface of the golden apple snail eggs using a sprayer until the surface of the golden apple snail eggs is moist and shiny. This means that the environmentally friendly spray will form a film on the surface of the golden apple snail eggs. When spraying, the spray nozzle should be kept 10-15cm away from the golden apple snail eggs. Spraying too close or too far away will affect the spraying effect. Step 4, Effect Verification: If the golden apple snail eggs turn bright red within 24 hours of spraying and the surface membrane is penetrated, the spraying is effective. The mortality rate of golden apple snail eggs is not less than 80%. After 72 hours of spraying, the eggshells of the golden apple snails harden, the membrane dries and coagulates, and the egg color turns pink. The conventional incubation time for golden apple snail eggs is generally about 2 weeks. After spraying 1-week-old eggs with an environmentally friendly spray for 1 week, the golden apple snails do not hatch and die. If the surface membrane of the golden apple snail eggs does not change color within 72 hours of spraying, the surface has not been penetrated, the spraying is ineffective, and Step 3 needs to be repeated to spray the golden apple snails again.

[0023] Before repeating step 3, the pH of the environmentally friendly spray for golden apple snail eggs needs to be tested. If the pH test strip shows a pH value of 5-6, the spray is in normal condition and can continue to be used; otherwise, it cannot be used and needs to be prepared again. The chitosan and sodium chloride in the environmentally friendly spray degrade quickly, requiring only 7-15 days. The frequency of use should not exceed 3 times per quarter to avoid excessive use and impacting the ecological environment. Furthermore, the used environmentally friendly spray must not be directly poured into fishponds or rivers, as the tea saponin in the spray has low toxicity and high tolerance to fish, with a low LC50 (lethal dose). 50 <1mg / L, therefore it needs to be rendered harmless. Particulate matter in the bottom sediment of the water body can adsorb some tea saponins, making them accumulate on the surface of the bottom sediment where microorganisms are active, thus indirectly improving the biodegradation efficiency.

[0024] Example 3, a method for controlling golden apple snail eggs, includes the following steps: Step 1, Spraying time: Spray with an environmentally friendly spray for golden apple snail eggs when the eggs are bright red, that is, 5-7 days before the golden apple snail eggs hatch. At the same time, the spraying time should be chosen at noon. During the day, the strong light will easily affect the evaporation and the spraying effect, while at night, the light is weak and it is not conducive to the work. Step 2, Spraying preparation: Before spraying, staff must wear masks, gloves and goggles. Tea saponin and acetic acid in the environmentally friendly spray can easily enter the respiratory tract or adhere to the skin surface during spraying, thus causing irritation. Therefore, protective measures are necessary. Slowly pour the prepared golden apple snail eggs into the sprayer using the environmentally friendly spray and let it stand for at least 10 minutes. Step 3, Preliminary treatment: Use a brush to clean the surface of the golden apple snail eggs. Lightly brushing can break the mucus layer on the surface of the golden apple snail eggs, which can improve the penetration effect of the environmentally friendly spray and achieve a penetration rate of more than 20%. Step 4, Spraying method: Spray the golden apple snail eggs onto the surface of the eggs with a sprayer until the surface of the golden apple snail eggs is moist and shiny. The environmentally friendly spray will form a film on the surface of the golden apple snail eggs. When spraying, the spray nozzle should be 10-15cm away from the golden apple snail eggs. Spraying too close or too far away will affect the spraying effect. Step 5, Effect Verification: If the golden apple snail eggs turn bright red within 24 hours of spraying and the surface membrane is penetrated, the spraying is effective. The mortality rate of golden apple snail eggs is not less than 80%. After 72 hours of spraying, the eggshells of the golden apple snails harden, the membrane dries and coagulates, and the egg color turns pink. The conventional incubation time for golden apple snail eggs is generally about 2 weeks. After spraying 1-week-old eggs with an environmentally friendly spray for 1 week, the golden apple snails do not hatch and die. If the surface membrane of the golden apple snail eggs does not change color within 72 hours of spraying, the surface has not been penetrated, the spraying is ineffective, and step 3 needs to be repeated to spray the golden apple snails again.

[0025] Before repeating step 4, the pH of the environmentally friendly spray for golden apple snail eggs needs to be tested. If the pH test strip shows a pH of 5-6, the spray is in normal condition and can continue to be used. Otherwise, it cannot be used and needs to be re-formulated. The chitosan and sodium chloride in the environmentally friendly spray degrade quickly, requiring only 7-15 days. The frequency of use should not exceed 3 times per quarter to avoid excessive use and impact on the ecological environment. In addition, the used environmentally friendly spray must not be poured directly into fish ponds or rivers, as the tea saponin in the environmentally friendly spray has low toxicity / high tolerance to fish, and the lethal dose (LC50) is low. 50 <1mg / L, therefore it needs to be rendered harmless. Particulate matter in the bottom sediment of the water body can adsorb some tea saponins, making them accumulate on the surface of the bottom sediment where microorganisms are active, thus indirectly improving the biodegradation efficiency.

[0026] In summary, the present invention has the following beneficial effects: The chitosan in the environmentally friendly spray forms a film on the surface of the golden apple snail eggs, preventing them from respiring and causing them to suffocate and die, thus achieving the killing effect. At the same time, the sodium chloride in the environmentally friendly spray increases the osmotic pressure of the golden apple snail eggs, causing them to dehydrate and die, also achieving the killing effect. In addition, the tea saponin in the environmentally friendly spray dissolves the mucus layer on the surface of the golden apple snail eggs and has a certain degree of toxicity, causing them to die, thus achieving the killing effect. Because the above three methods of killing golden apple snails work together, the effectiveness of the environmentally friendly spray in killing golden apple snail eggs is effectively guaranteed.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the design concept of the present invention should be included within the protection scope of the present invention.

Claims

1. An environmentally friendly spray for inhibiting the hatching of golden apple snail eggs, comprising an environmentally friendly spray, characterized in that, The environmentally friendly spray contains chitosan, acetic acid, sodium chloride, tea saponin, organosilicon additives, and solvents.

2. The environmentally friendly spray for inhibiting the hatching of golden apple snail eggs according to claim 1, characterized in that, When the environmentally friendly spray is prepared in a volume of 10,000 ml, it is prepared by using 50 g chitosan, 150 ml acetic acid, 50 g sodium chloride, 50 g tea saponin, and 10-20 ml organosilicon additives, and an appropriate amount of solvent is selected so that the total volume of liquid reaches 10,000 ml.

3. An environmentally friendly spray for inhibiting the hatching of golden apple snail eggs according to claim 1 or 2, characterized in that, The preparation of the environmentally friendly spray includes the following steps: Step 1: Take container A and put 2000ml of purified water in container A. The temperature of the solvent is controlled at 40-50℃. Then, select 5% acetic acid and add 150ml of 5% acetic acid to container A and stir evenly to obtain mixed solution I. Step 2: Slowly add 50g of chitosan to solution I in container A. Water-soluble chitosan is selected. Since the acetic acid added in step 1 can provide a weak acid environment to promote the dissolution of chitosan, stir continuously at a speed of 300-500r / min for 30min until the chitosan is completely dissolved to obtain mixed solution II. At this time, solution II is transparent and slightly viscous. Step 3: Take container B and put 3000ml of purified water in container B. Then add 50g of sodium chloride to bottle B and stir well to obtain mixed solution III. Adding sodium chloride can increase the osmotic pressure and destroy the embryonic cells in the golden apple snail egg, causing it to dehydrate and die. Step 4: Add 50g of tea saponin to solution III in container B and stir well to obtain mixed solution IV. When tea saponin is sprayed on the surface of golden apple snail eggs, it can dissolve the mucus layer on the surface of the egg mass and enhance the penetration of the environmentally friendly spray. Step 5: Add silicone additive to solution IV in container B and stir evenly to obtain mixed solution V; 10-20 ml of silicone additive can be used. Silicone additive can reduce surface tension and make the environmentally friendly spray evenly adhere to the crevices on the surface of golden apple snail eggs. Step 6: Slowly pour the solution II obtained in step 2 into the solution V obtained in step 5, stirring evenly while pouring, to obtain a mixed solution VI; Step 7: Add an appropriate amount of purified water to solution VI obtained in step 6 and stir well so that the total volume of the mixed liquid reaches 10000ml. Let it stand for 10 minutes to obtain an environmentally friendly spray that inhibits the hatching of golden apple snail eggs.

4. A method for using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs, characterized in that, This includes spraying the surface of the golden apple snail with an environmentally friendly spray that inhibits the hatching of golden apple snail eggs as described in claim 3.

5. The method of using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs according to claim 4, characterized in that, The steps include the following: Step 1, Spraying time: Spray with an environmentally friendly spray when the golden apple snail eggs are bright red, that is, 5-7 days before the golden apple snail eggs hatch. At the same time, the spraying time should be noon. Step 2, Spraying Preparation: Before spraying, staff must wear masks, gloves and goggles, slowly pour the prepared environmentally friendly spray into the sprayer and let it stand for at least 10 minutes; Step 3, Spraying method: Spray the golden apple snail eggs onto the surface of the eggs with a sprayer until the surface of the golden apple snail eggs is moist and shiny. When spraying, the spray nozzle should be 10-15cm away from the golden apple snail eggs. Step 4, Effect Verification: If the golden apple snail eggs turn bright red within 24 hours of spraying and the surface membrane is penetrated, the spraying is effective. The mortality rate of the golden apple snail eggs is not less than 80%. After 72 hours of spraying, the eggshells of the golden apple snails harden, the membrane dries and solidifies, and the egg color turns pink. If the surface membrane of the golden apple snail eggs does not change color within 72 hours of spraying, the surface has not been penetrated, the spraying is ineffective, and Step 3 needs to be repeated to spray the golden apple snails again.

6. The method of using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs according to claim 4, characterized in that, The steps include the following: Step 1, Spraying time: Spray with an environmentally friendly spray when the golden apple snail eggs are bright red, that is, 5-7 days before the golden apple snail eggs hatch. At the same time, the spraying time should be noon. Step 2, Spraying Preparation: Before spraying, staff must wear masks, gloves and goggles, slowly pour the prepared environmentally friendly spray into the sprayer and let it stand for at least 10 minutes; Step 3, Preliminary treatment: Clean the surface of the golden apple snail eggs with a brush; Step 4, Spraying method: Spray the golden apple snail eggs onto the surface of the eggs with a sprayer until the surface of the golden apple snail eggs is moist and shiny. When spraying, the spray nozzle should be 10-15cm away from the golden apple snail eggs. Step 5, Effect Verification: If the golden apple snail eggs turn bright red within 24 hours of spraying and the surface membrane is penetrated, the spraying is effective. The mortality rate of the golden apple snail eggs is not less than 80%. After 72 hours of spraying, the eggshells of the golden apple snails harden, the membrane dries and solidifies, and the egg color turns pink. If the surface membrane of the golden apple snail eggs does not change color within 72 hours of spraying, the surface has not been penetrated, the spraying is ineffective, and step 4 needs to be repeated to spray the golden apple snails again.

7. A method for using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs, as described in claim 5 or 6, characterized in that, Before re-spraying the golden apple snail eggs, a pH test is performed using an environmentally friendly spray. If the pH test strip shows a pH value of 5-6, the environmentally friendly spray is in a normal state and can continue to be used; otherwise, it cannot be used and needs to be re-prepared.

8. A method for using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs, as described in claim 5 or 6, characterized in that, The environmentally friendly spray should be used no more than 3 times per quarter.

9. A method for using an environmentally friendly spray based on inhibiting the hatching of golden apple snail eggs, as described in claim 5 or 6, characterized in that, The environmentally friendly spray must not be poured directly into fishponds or rivers after use; it must be disposed of in a harmless manner.