Soluble powder containing 28-homobrassinolide
By adding nerol and specific auxiliary ingredients to the soluble powder, the degradation problem of 28-homobrassinolide during storage was solved, the stability of the active ingredient and the growth-promoting effect on Chinese cabbage were improved, and the formulation was able to be rapidly dispersed and sprayed evenly in high humidity environments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-30
- Publication Date
- 2026-04-10
Smart Images
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of soluble powder technology, specifically relating to a soluble powder containing 28-homobrassinolide. Background Technology
[0002] In agricultural production, the growth rate, yield, and quality of bok choy, a common leafy vegetable, directly affect planting efficiency. 28-Homobrassinolide, a highly efficient and broad-spectrum plant growth regulator, has been widely used in bok choy cultivation due to its ability to promote plant cell division and elongation and enhance photosynthesis. It has become one of the important technical means to improve the growth status and increase the yield of bok choy. Meanwhile, considering the ease of use, dispersibility, and stability of pesticide formulations, soluble powders, with their advantages of easy dispersion in water and convenient storage and transportation, have become the mainstream formulation of 28-homobrassinolide in bok choy applications.
[0003] Current research on 28-homobrassinolide soluble powder for pakchoi primarily focuses on achieving basic formulation functions. This means ensuring basic performance through the combination of dispersants, wetting agents, and fillers, while neglecting the active ingredient itself and the formulation's overall effectiveness. As a sterol compound, 28-homobrassinolide is susceptible to degradation due to temperature during storage, leading to a decrease in active ingredient content within the formulation's shelf life and weakening its growth-regulating effect on pakchoi. Furthermore, the growth-promoting effect of 28-homobrassinolide on pakchoi is limited. Therefore, it is necessary to consider adding functional adjuvants to improve the formulation's growth-promoting effect on pakchoi while ensuring formulation stability.
[0004] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention
[0005] The purpose of this invention is to provide a soluble powder containing 28-homobrassinolide to solve the problems existing in the prior art.
[0006] To achieve the above objectives, the present invention provides the following technical solution: A soluble powder, by mass percentage, is prepared from the following components: 0.01-0.1% active ingredient, 6-18% dispersant, 3-8% wetting agent, 0.5-3% functional additive, and filler to 100%; wherein the active ingredient is 28-homobrassinolide; and the functional additive is nerol.
[0007] Furthermore, the dispersant is sodium lignosulfonate.
[0008] Furthermore, the wetting agent is a mixture of sodium dodecyl sulfate and sodium lauroyl glutamate.
[0009] Furthermore, the mass ratio of sodium dodecyl sulfate to sodium lauroyl glutamate is 1-3:3.
[0010] Furthermore, the filler is anhydrous trehalose.
[0011] Furthermore, by weight percentage, the soluble powder is prepared from the following components: 0.05% active ingredient, 12% dispersant, 6% wetting agent, 1.5% functional additive, and filler to 100%.
[0012] The present invention also provides a method for preparing the soluble powder, comprising the following steps: S1. Dissolve the active ingredients and functional additives in anhydrous ethanol to obtain the mother liquor; S2. The filler is crushed and passed through an 80-mesh sieve; the mother liquor of S1 is evenly sprayed onto the filler powder, stirred and mixed at 30 r / min for 30 min and then allowed to stand for 20 min; S3. Add the dispersant and wetting agent in sequence, stir and mix at 40 r / min for 60 min, then air-jet pulverize through a 45 μm sieve to obtain the soluble powder.
[0013] The present invention also provides the application of the soluble powder in promoting the growth of Chinese cabbage and improving its yield and quality.
[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention uses nerol as a functional adjuvant, which can effectively inhibit the pyrolysis of 28-homobrassinolide, so that the activity retention rate of the preparation after heat storage reaches more than 95%; at the same time, nerol can produce a synergistic effect with 28-homobrassinolide, which can significantly enhance the promoting effect of 28-homobrassinolide on the growth of Chinese cabbage.
[0015] (2) The present invention uses sodium dodecyl sulfate and sodium lauroyl glutamate as a wetting agent, and with the assistance of nerol, the wetting time of the formulation can be significantly shortened, ensuring rapid dispersion after entering the water; at the same time, nerol and anhydrous trehalose work synergistically to significantly reduce the moisture absorption rate of the formulation, solving the problem of easy moisture absorption and clumping in high humidity environment, and can also help reduce the foam volume during spraying, improving the convenience of field use. Detailed Implementation
[0016] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.
[0017] 1. Synergistic effect of nerol on 28-homobrassinolide 1.1 The tested bok choy Shanghai Qing 1.2 Test reagents 95% 28-homobrassinolide technical grade, 98% nerol technical grade; After dissolving in a solvent, the solution was diluted with a 0.1% Tween-80 aqueous solution to form a single-agent stock solution. Based on the results of the preliminary test, multiple formulations were set up, and each single-agent stock solution and formulation mixture was diluted with a 0.1% Tween-80 aqueous solution to form 5 mass concentration gradients.
[0018] 1.3 Test methods (refer to NY / T 2061.2-2011 Guidelines for Indoor Bioassay of Pesticides - Plant Growth Regulators - Part 2: Foliar Spray Method for Promoting / Inhibiting Plant Growth).
[0019] Take soil from the field, dry it in the shade, crush it and pass it through a 20-mesh sieve. Fill pots to 4 / 5 full and thoroughly moisten the soil using bottom irrigation. Sow pak choi seeds on the soil surface, cover with 0.5cm of soil, 1 seed per pot. When seedlings reach the 2-heart, 1-leaf stage, select seedlings with uniform growth for spraying. Each treatment is replicated 3 times, with 3 pots per replicate. A control group is included, sprayed with an equal amount of water. 15 days after spraying, the fresh weight of the above-ground parts of the pak choi is measured, and the growth-promoting rate of the pesticide on the pak choi is calculated based on the results.
[0020]
[0021] 1.4 Data Processing A regression equation was established between the probability value of the growth promotion rate of the aboveground parts by the pesticide and the logarithm of the pesticide concentration, and the EC50 of the pesticide was calculated. 50 The results are shown in Table 1.
[0022] Table 2. Effects of nerol combined with 28-homobrassinolide on the fresh weight of aboveground parts of Chinese cabbage. Note: In Table 1, "--" indicates that nerol at 8000 mg / L has no promoting effect.
[0023] Table 1 shows that 28-homobrassinolide alone promotes the growth of the aboveground fresh weight of pak choi. 50The concentration of nerol was 0.086 mg / L. At 8000 mg / L, nerol alone had no effect on promoting the fresh weight growth of the above-ground parts of pak choi. However, when nerol was used in combination with 28-homobrassinolide, certain proportions of the combination showed a significant promoting effect on the fresh weight growth of the above-ground parts of pak choi, especially when the mass ratio of 28-homobrassinolide to nerol was 0.05:1.5, which significantly promoted the growth of the above-ground parts of pak choi. 50 The value was 0.024 mg / L, significantly lower than 0.086 mg / L, meaning that nerol can significantly enhance the growth-promoting effect of 28-homobrassinolide on pak choi.
[0024] 2. The Influence of Different Auxiliary Components on the Performance of Soluble Powders 2.1 Soluble Formulation See Table 2.
[0025] Table 2. Soluble Powder Formulations (Unit: kg)
[0026] 2.2 Soluble Powder The preparation method of the soluble powder in Table 2 includes the following steps: S1. Take 3 kg of anhydrous ethanol and place it in a container. Add 28-homobrassinolide and stir at 200 r / min for 15 min. Add nerol and stir at 200 r / min for 10 min to obtain the mother liquor. S2. Grind anhydrous trehalose through an 80-mesh sieve; spray the mother liquor of S1 evenly onto the anhydrous trehalose powder, stir and mix at 30 r / min for 30 min and let stand for 20 min; S3. Add sodium lignosulfonate, sodium dodecyl sulfate and sodium lauroyl glutamate in sequence, stir and mix at 40 r / min for 60 min, then air-jet pulverize through a 45 μm sieve to obtain the soluble powder.
[0027] 2.3 Performance Testing The test metrics are shown in Table 3, and the test results are shown in Table 4. Table 3. Test Items and Test Methods
[0028] Table 3. Effects of different additives on the properties of soluble powders
[0029] As shown in Table 3, in terms of thermal storage stability, the retention rate of 28-homobrassinolide after thermal storage of K4-K9 was significantly higher than that of K1-K3, indicating that nerol can effectively inhibit the pyrolysis of 28-homobrassinolide. Moreover, within the range of 0.5-1.5%, the stabilizing effect increases with the increase of dosage, with the best effect at 1.5%, and the stabilizing effect decreases from 2.0-3.0%.
[0030] Regarding hygroscopicity, the hygroscopicity of K1-K3 without nerol is 8.9-11.5%, which decreases to 4.9-7.3% after the addition of nerol, with K6 at only 5.1%. Combined with the anti-caking effect of anhydrous trehalose, it can effectively solve the problem of easy moisture absorption and clumping in humid environments.
[0031] In terms of sustained foaming performance, K6 foam volume was only 4.8 mL, far lower than K3's 8.3 mL, indicating that the weak hydrophobicity of nerol can help reduce foaming when compounded with wetting agents, thus preventing foam from affecting the uniformity of adhesion during spraying. Regarding wetting properties, the wetting times of K1 and K2 were 68s and 72s, respectively, while the wetting times of the compound groups K3-K9 were all less than 43s, and were further shortened to 37-40s after the addition of nerol. This indicates that there is a synergistic effect between the two wetting agents. The addition of nerol can further optimize the wetting effect, ensure rapid dispersion of the formulation after it enters the water, and meet the needs of field spraying.
[0032] In summary, K6 is the preferred formulation, exhibiting high activity retention after heat storage, short wetting time, low persistent foaming, and low moisture absorption.
[0033] In summary, the present invention uses nerol as a functional adjuvant, which can effectively inhibit the pyrolysis of 28-homobrassinolide, so that the activity retention rate of the preparation after heat storage reaches more than 95%; at the same time, nerol can produce a synergistic effect with 28-homobrassinolide, which can significantly enhance the promoting effect of 28-homobrassinolide on the growth of pak choi.
[0034] In addition, this invention uses sodium dodecyl sulfate and sodium lauroyl glutamate as a wetting agent, and with the assistance of nerol, the wetting time of the formulation can be significantly shortened, ensuring rapid dispersion after entering the water. At the same time, the synergistic effect of nerol and anhydrous trehalose can significantly reduce the moisture absorption rate of the formulation, solving the problem of easy moisture absorption and clumping in high humidity environments, and can also help reduce the foam volume during spraying, improving the convenience of field use.
[0035] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.
Claims
1. A dissolvable powder, characterized in that, The soluble powder is prepared from the following ingredients in percentage by mass: active ingredient 0.01-0.1%, dispersant 6-18%, wetting agent 3-8%, functional adjuvant 0.5-3%, and filler supplementing to 100%; wherein the active ingredient is 28-epi-brassinolide; and the functional adjuvant is orange flower alcohol.
2. The dissolvable powder of claim 1, wherein, The dispersant is sodium lignosulfonate.
3. The dissolvable powder of claim 1, wherein, The wetting agent is a mixture of sodium dodecyl sulfate and sodium lauroyl glutamate.
4. The dissolvable powder of claim 3, wherein, The mass ratio of sodium dodecyl sulfate to sodium lauroyl glutamate is 1-3:
3.
5. The dissolvable powder of claim 1, wherein, The filler is anhydrous trehalose.
6. The dissolvable powder of claim 1, wherein, The soluble powder is prepared from the following ingredients in percentage by mass: active ingredient 0.05%, dispersant 12%, wetting agent 6%, functional adjuvant 1.5%, and filler supplementing to 100%.
7. A process for the preparation of the soluble powder according to any one of claims 1 to 6, characterized in that, The method comprises the following steps: S1. dissolving the active ingredient and the functional adjuvant with anhydrous ethanol to obtain a mother liquor; S2. crushing the filler through an 80-mesh sieve; uniformly spraying the S1 mother liquor onto the filler powder, stirring and mixing at 30 r / min for 30 min and standing for 20 min; S3. sequentially adding the dispersant and the wetting agent, stirring and mixing at 40 r / min for 60 min, and airflow crushing through a 45-μm sieve to obtain the soluble powder.
8. The use of the soluble powder according to any one of claims 1-6 in promoting the growth of Chinese flowering cabbage, improving the yield and quality of Chinese flowering cabbage.