Disinfectant composition and preparation method thereof
By using a multi-component synergistic formulation of glutaraldehyde, o-phthalaldehyde, peracetic acid, and other components, the problem of poor spore-killing effect of existing chemical disinfectants has been solved, achieving a highly efficient effect in killing microorganisms and spores.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-08
- Publication Date
- 2026-04-10
AI Technical Summary
The effectiveness of existing chemical disinfectants in killing dormant bacterial spores and other bacterial cells needs to be improved.
The formulation employs a multi-component synergistic approach, including glutaraldehyde, o-phthalaldehyde, peracetic acid, ethanol, and elemental iodine. Through mechanisms such as alkylation, cross-linking, oxidation, and membrane disruption, it enhances the killing effect on microorganisms, particularly the killing rate of spores.
It achieved a kill rate of over 99.999% for surface microorganisms and over 99% for spores, significantly improving the killing effect on dormant organisms such as spores.
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Figure CN121817184A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of disinfectant technology, specifically to a disinfectant composition and its preparation method. Background Technology
[0002] Chemical disinfection is a method of killing pathogenic microorganisms by using chemical agents to denature or coagulate their proteins, inactivate their enzyme systems, or damage their cell membranes. The core sterilization mechanisms of this method include protein denaturation, enzyme system interference, and alteration of cell membrane permeability. Commonly used disinfectants include chlorine-based disinfectants, peroxides, iodine-based disinfectants, and alcohols. These disinfectants vary significantly in their bactericidal ability, applicability, and side effects. Although this method has limitations such as strong corrosiveness and residual toxicity, its ease of operation and variety make it an important sterilization method in medical institutions and daily life.
[0003] Chemical disinfectants primarily inactivate pathogenic microorganisms through the following three mechanisms: Protein denaturation or coagulation: Heavy metal salts, aldehydes, and alcohols disrupt the spatial structure of microbial proteins, causing them to lose their physiological functions. Enzyme system interference: Oxidizing disinfectants bind to sulfhydryl groups in bacterial enzyme systems, blocking enzyme catalytic activity and leading to metabolic termination. Cell membrane alterations: Surfactant disinfectants reduce cell membrane surface tension, increase permeability, and trigger the outflow or lysis of cell contents.
[0004] Currently, mainstream chemical disinfectants can be categorized into chlorine-based disinfectants, peroxide disinfectants, iodine-based disinfectants, and alcohol-based disinfectants. Chlorine-based disinfectants, represented by sodium hypochlorite and bleaching powder, have broad-spectrum bactericidal capabilities and can effectively inactivate vegetative bacteria, viruses, and fungal spores. However, these disinfectants are corrosive to metal products, and high concentrations can cause environmental pollution. Peroxide disinfectants, mainly including peracetic acid and ozone, interfere with bacterial enzyme systems and metabolism through strong oxidation, making them suitable for medical device and air disinfection. Their disadvantages include chemical instability, requiring immediate preparation, and potential for degradation during long-term storage. Iodine-based disinfectants, represented by povidone-iodine, have low toxicity and are suitable for skin and mucous membrane disinfection, but their effectiveness in inactivating bacterial spores is limited, and they are corrosive to metals such as silver and copper. Alcohol-based disinfectants, mainly ethanol and isopropanol, are effective against vegetative bacteria and some viruses, and are commonly used for skin surface disinfection; however, these disinfectants cannot kill spores, and concentrations exceeding 80% can reduce their bactericidal effect due to rapid dehydration. Summary of the Invention
[0005] The present invention aims to address the technical deficiencies of the prior art by providing a disinfectant composition and its preparation method, thereby solving the technical problem that the killing effect of conventional chemical disinfectants on dormant bacterial spores and other bacterial cells needs to be improved.
[0006] To achieve the above technical objectives, the present invention adopts the following technical solution: A disinfectant composition comprising glutaraldehyde, o-phthalaldehyde, peracetic acid, ethanol, elemental iodine, fluopyram, difenoconazole, pyraclostrobin, polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, dodecylbenzenesulfonic acid, tartaric acid, calcium stearate, and disodium EDTA.
[0007] Preferably, the disinfectant composition comprises the following components in parts by weight: 18-22 parts glutaraldehyde, 15-20 parts o-phthalaldehyde, 12-15 parts peracetic acid, 12-15 parts ethanol, 6-8 parts elemental iodine, 3-6 parts fluopyram, 3-7 parts difenoconazole, 1-2 parts pyraclostrobin, 3-5 parts polyhexamethylene biguanide, 1-3 parts sodium dichloroisocyanurate, 0.8-2 parts sodium nitrite, 1-2 parts dodecylbenzenesulfonic acid, 1-3 parts tartaric acid, 1-3 parts calcium stearate, and 2-4 parts disodium EDTA.
[0008] Preferably, the disinfectant composition comprises the following components in parts by weight: glutaraldehyde 19-21 parts, o-phthalaldehyde 17-19 parts, peracetic acid 13-14 parts, ethanol 13-14 parts, elemental iodine 6.5-7.5 parts, fluopyram 4-5 parts, difenoconazole 4-6 parts, pyraclostrobin 1.3-1.6 parts, polyhexamethylene biguanide 3.5-4.5 parts, sodium dichloroisocyanurate 1.5-2.5 parts, sodium nitrite 1.2-1.6 parts, dodecylbenzenesulfonic acid 1.3-1.7 parts, tartaric acid 1.5-2.5 parts, calcium stearate 1.5-2.5 parts, and disodium EDTA 2.5-3.5 parts.
[0009] Preferably, the disinfectant composition comprises the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, and 3 parts disodium EDTA.
[0010] Preferably, the disinfectant composition also includes 1 to 2 parts by weight of sodium thiosulfate.
[0011] Preferably, the disinfectant composition also includes 2 to 3 parts by weight of potassium iodide.
[0012] Preferably, the disinfectant composition further includes 0.5 to 1 part by weight of polyhexamethylene biguanide.
[0013] Preferably, the disinfectant composition also includes 1 to 2 parts by weight of zinc molybdate.
[0014] Preferably, the disinfectant composition comprises the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, 3 parts disodium EDTA, 1.5 parts sodium thiosulfate, 2.5 parts potassium iodide, 0.7 parts polyhexamethylene biguanide, and 1.5 parts zinc molybdate.
[0015] Based on the above technical solutions, the present invention further provides a method for preparing the above-mentioned disinfectant composition, comprising the following steps: taking the prescribed amounts of glutaraldehyde, o-phthalaldehyde, and peracetic acid and stirring them at room temperature until homogeneous; then, while continuing to stir, adding the prescribed amounts of ethanol and elemental iodine; adjusting the system temperature to 45-50°C, and sequentially dispersing the prescribed amounts of fluopyram, difenoconazole, and pyraclostrobin into the system, and ultrasonically vibrating for 20-30 minutes; dissolving the prescribed amounts of polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, and dodecylbenzenesulfonic acid in water, and adding the resulting solution dropwise into the ultrasonically vibrated system; adjusting the temperature to 25-30°C, and adding the prescribed amounts of tartaric acid, calcium stearate, and disodium EDTA, and stirring until homogeneous.
[0016] In the above technical solutions, glutaraldehyde is a highly efficient and broad-spectrum chemical disinfectant. In the field of disinfection, it mainly works by alkylating the aldehyde groups in its molecular structure with the proteins of microorganisms, thereby inactivating the enzymes and structural proteins of microorganisms and leading to their death. Ortho-phthalaldehyde (OPA) is a highly efficient chemical disinfectant. Its core function is to achieve sterilization or disinfection by cross-linking with the proteins and nucleic acids of microorganisms, destroying their structure and function. As an aldehyde compound, it has broad-spectrum bactericidal activity and can effectively kill vegetative bacteria, fungi, mycobacteria (including tuberculosis bacilli), viruses (such as enteroviruses and hepatitis B viruses), and even bacterial spores. Peracetic acid decomposes to produce free radicals or reactive oxygen species. These active components can damage the cell membranes, protein structures, and enzyme systems of microorganisms, leading to their death. Simultaneously, peracetic acid can also interfere with the replication of microbial DNA or RNA, further inhibiting their reproduction. Ethanol primarily achieves its bactericidal and disinfecting effects by disrupting the protein structure of microorganisms and dissolving their lipid membranes. It can rapidly penetrate the cell membranes or capsids of bacteria, viruses, and other microorganisms, causing protein denaturation and coagulation, thereby leading to microbial death. Elemental iodine can directly oxidize the protein structure of pathogens, destroying their active groups and causing denaturation of the pathogen's enzymes and amino acids, thus achieving a killing effect. This oxidative action can broadly kill a variety of microorganisms.
[0017] Fluopyram is an amide-based fungicide that works by inhibiting the formation of pathogenic cell membranes; difenoconazole is a highly effective, safe, low-toxicity, broad-spectrum fungicide; pyraclostrobin inhibits or kills pathogens by interfering with microbial respiration and affecting their energy metabolism. These components, in synergy with fungicides such as glutaraldehyde, can significantly inhibit microbial regeneration and improve the long-lasting effect of disinfectants. Polyhexamethylene biguanide and sodium dichloroisocyanurate further enhance the sterilization effect. Sodium nitrite and tartaric acid provide some rust prevention. Dodecylbenzenesulfonic acid and calcium stearate improve the surface adhesion of the disinfectant. Disodium EDTA acts as a chelating agent to improve the stability of the disinfectant.
[0018] This invention provides a disinfectant composition and its preparation method. This technical solution constructs a multi-component synergistic disinfectant formulation, using glutaraldehyde, o-phthalaldehyde, and ethanol as core bactericidal components, and adding fluopyram and pyraclostrobin to achieve antibacterial effects, thus realizing the broad-spectrum and long-lasting effect of the disinfectant. Furthermore, this invention exhibits good surface adhesion and penetration, achieving a kill rate of over 99.999% against surface microorganisms and over 99% against dormant bacterial spores and other microorganisms. This invention demonstrates excellent bactericidal effect and significant technical advantages. Attached Figure Description
[0019] Figure 1 This is a statistical result chart of the surface sterilization effect of various embodiments and comparative examples of the present invention. Detailed Implementation
[0020] The specific embodiments of the present invention will be described in detail below. To avoid excessive and unnecessary detail, well-known structures or functions will not be described in detail in the following embodiments. The approximate language used in the following embodiments is for quantitative purposes, indicating that a certain degree of variation in quantity is permissible without changing the basic function. Unless otherwise defined, the technical and scientific terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which this invention pertains.
[0021] Example 1 A disinfectant composition comprising the following components in parts by weight: 18 parts glutaraldehyde, 15 parts o-phthalaldehyde, 12 parts peracetic acid, 12 parts ethanol, 6 parts iodine, 3 parts fluopyram, 3 parts difenoconazole, 1 part pyraclostrobin, 3 parts polyhexamethylene biguanide, 1 part sodium dichloroisocyanurate, 0.8 parts sodium nitrite, 1 part dodecylbenzenesulfonic acid, 1 part tartaric acid, 1 part calcium stearate, and 2 parts disodium EDTA. The preparation method is as follows: Glutaraldehyde, o-phthalaldehyde, and peracetic acid of the prescribed amounts are stirred and mixed at room temperature. Then, while continuing to stir, ethanol and iodine of the prescribed amounts are added. The system temperature is adjusted to 48°C, and fluopyram, difenoconazole, and pyraclostrobin of the prescribed amounts are dispersed into the system sequentially. The system is then ultrasonically vibrated for 25 minutes. Polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, and dodecylbenzenesulfonic acid of the prescribed amounts are dissolved in water, and the resulting solution is added dropwise to the ultrasonically vibrated system. The temperature is adjusted to 27°C, and tartaric acid, calcium stearate, and disodium EDTA of the prescribed amounts are added and stirred until well mixed. This example was used to wipe plastic and metal surfaces, and the total number of microorganisms, spores, and dinoflagellates before and after wiping were measured to calculate the kill rate. The results showed that when applied to plastic surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.23%, and the dinoflagellate cyst kill rate was 99.65%; when applied to metal surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.95%, and the dinoflagellate cyst kill rate was 99.97%.
[0022] Example 2 A disinfectant composition comprising the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, and 3 parts disodium EDTA. The preparation method is the same as in Example 1. Using this example, plastic and metal surfaces were wiped, and the total number of microorganisms, spores, and dinoflagellates before and after wiping were measured to calculate the kill rate. The results showed that when applied to plastic surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.56%, and the dinoflagellate cyst kill rate was 99.81%; when applied to metal surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.98%, and the dinoflagellate cyst kill rate was 99.99%.
[0023] Example 3 A disinfectant composition comprising the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, 3 parts disodium EDTA, 1 part sodium thiosulfate, 2 parts potassium iodide, 0.5 parts polyhexamethylene biguanide, and 1 part zinc molybdate. The preparation method is as follows: Glutaraldehyde, o-phthalaldehyde, and peracetic acid of the prescribed amounts are stirred and mixed at room temperature. Then, while continuing to stir, ethanol and iodine of the prescribed amounts are added. The system temperature is adjusted to 48℃, and fluopyram, difenoconazole, and pyraclostrobin of the prescribed amounts are dispersed into the system sequentially. The system is then ultrasonically vibrated for 25 minutes. Polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, and dodecylbenzenesulfonic acid of the prescribed amounts are dissolved in water, and the resulting solution is added dropwise to the ultrasonically vibrated system. The temperature is adjusted to 27℃, and tartaric acid, calcium stearate, disodium EDTA, sodium thiosulfate, potassium iodide, polyhexamethylene biguanide, and zinc molybdate of the prescribed amounts are added and stirred until well mixed. This example was used to wipe plastic and metal surfaces, and the total number of microorganisms, spores, and dinoflagellates before and after wiping were measured to calculate the kill rate. The results showed that when applied to plastic surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.78%, and the dinoflagellate cyst kill rate was 99.92%; when applied to metal surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.99%, and the dinoflagellate cyst kill rate was 99.99%.
[0024] Example 4 A disinfectant composition comprising the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, 3 parts disodium EDTA, 1.5 parts sodium thiosulfate, 2.5 parts potassium iodide, 0.7 parts polyhexamethylene biguanide, and 1.5 parts zinc molybdate. The preparation method is the same as in Example 3. Using this example, plastic and metal surfaces were wiped, and the total number of microorganisms, spores, and dinoflagellates before and after wiping were measured to calculate the kill rate. The results showed that when applied to plastic surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.99%, and the dinoflagellate cyst kill rate was 99.99%; when applied to metal surfaces, the total microbial kill rate was 99.999%, the spore kill rate was 99.99%, and the dinoflagellate cyst kill rate was 99.99%.
[0025] Comparative Example 1 Commercially available dichlorohydantoin disinfectant was used. This embodiment was applied to wipe plastic and metal surfaces, and the total number of microorganisms, spores, and dinoflagellates were measured before and after wiping to calculate the kill rate. The results showed that when used on plastic surfaces, the total microorganism kill rate was 99.832%, the spore kill rate was 73.25%, and the dinoflagellate kill rate was 84.36%; when used on metal surfaces, the total microorganism kill rate was 99.981%, the spore kill rate was 79.38%, and the dinoflagellate kill rate was 87.30%.
[0026] Comparative Example 2 Commercially available benzalkonium bromide disinfectant, "Xin Jie Er Mie," was used. This embodiment was applied to wipe plastic and metal surfaces, and the total number of microorganisms, spores, and dinoflagellates were measured before and after wiping to calculate the kill rate. The results showed that when used on plastic surfaces, the kill rate of total microorganisms was 99.917%, the kill rate of spores was 80.16%, and the kill rate of dinoflagellates was 85.84%; when used on metal surfaces, the kill rate of total microorganisms was 99.952%, the kill rate of spores was 84.28%, and the kill rate of dinoflagellates was 89.14%.
[0027] The embodiments of the present invention have been described in detail above, but the content described is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the scope of the present invention should be included within the protection scope of the present invention.
Claims
1. A disinfectant composition, characterized in that... Including glutaraldehyde, o-phthalaldehyde, peracetic acid, ethanol, elemental iodine, fluopyram, difenoconazole, pyraclostrobin, polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, dodecylbenzenesulfonic acid, tartaric acid, calcium stearate, and disodium EDTA.
2. The disinfectant composition according to claim 1, characterized in that, The disinfectant composition comprises the following components in parts by weight: glutaraldehyde 18-22 parts, o-phthalaldehyde 15-20 parts, peracetic acid 12-15 parts, ethanol 12-15 parts, elemental iodine 6-8 parts, fluopyram 3-6 parts, difenoconazole 3-7 parts, pyraclostrobin 1-2 parts, polyhexamethylene biguanide 3-5 parts, sodium dichloroisocyanurate 1-3 parts, sodium nitrite 0.8-2 parts, dodecylbenzenesulfonic acid 1-2 parts, tartaric acid 1-3 parts, calcium stearate 1-3 parts, and disodium EDTA 2-4 parts.
3. The disinfectant composition according to claim 2, characterized in that, The disinfectant composition comprises the following components in parts by weight: glutaraldehyde 19-21 parts, o-phthalaldehyde 17-19 parts, peracetic acid 13-14 parts, ethanol 13-14 parts, elemental iodine 6.5-7.5 parts, fluopyram 4-5 parts, difenoconazole 4-6 parts, pyraclostrobin 1.3-1.6 parts, polyhexamethylene biguanide 3.5-4.5 parts, sodium dichloroisocyanurate 1.5-2.5 parts, sodium nitrite 1.2-1.6 parts, dodecylbenzenesulfonic acid 1.3-1.7 parts, tartaric acid 1.5-2.5 parts, calcium stearate 1.5-2.5 parts, and disodium EDTA 2.5-3.5 parts.
4. The disinfectant composition according to claim 3, characterized in that, The disinfectant composition comprises the following components in parts by weight: 20 parts glutaraldehyde, 18 parts o-phthalaldehyde, 13.5 parts peracetic acid, 13.5 parts ethanol, 7 parts elemental iodine, 4.5 parts fluopyram, 5 parts difenoconazole, 1.5 parts pyraclostrobin, 4 parts polyhexamethylene biguanide, 2 parts sodium dichloroisocyanurate, 1.5 parts sodium nitrite, 1.4 parts dodecylbenzenesulfonic acid, 2 parts tartaric acid, 2 parts calcium stearate, and 3 parts disodium EDTA.
5. The disinfectant composition according to claim 3, characterized in that, The disinfectant composition also includes 1 to 2 parts by weight of sodium thiosulfate.
6. The disinfectant composition according to claim 3, characterized in that, The disinfectant composition also includes 2 to 3 parts by weight of potassium iodide.
7. The disinfectant composition according to claim 3, characterized in that, The disinfectant composition also includes 0.5 to 1 part by weight of polyhexamethylene biguanide.
8. The disinfectant composition according to claim 3, characterized in that, The disinfectant composition also includes 1 to 2 parts by weight of zinc molybdate.
9. A disinfectant composition according to claim 3, characterized in that, The disinfectant composition comprises the following components in parts by weight: glutaraldehyde 20 parts, o-phthalaldehyde 18 parts, peracetic acid 13.5 parts, ethanol 13.5 parts, elemental iodine 7 parts, fluopyram 4.5 parts, difenoconazole 5 parts, pyraclostrobin 1.5 parts, polyhexamethylene biguanide 4 parts, sodium dichloroisocyanurate 2 parts, sodium nitrite 1.5 parts, dodecylbenzenesulfonic acid 1.4 parts, tartaric acid 2 parts, calcium stearate 2 parts, disodium EDTA 3 parts, sodium thiosulfate 1.5 parts, potassium iodide 2.5 parts, polyhexamethylene biguanide 0.7 parts, and zinc molybdate 1.5 parts.
10. A method for preparing the disinfectant composition according to any one of claims 1 to 9, characterized in that, The process includes the following steps: Mix the prescribed amounts of glutaraldehyde, o-phthalaldehyde, and peracetic acid at room temperature. Then, while continuing to stir, add the prescribed amounts of ethanol and elemental iodine. Adjust the system temperature to 45-50°C, and sequentially disperse the prescribed amounts of fluopyram, difenoconazole, and pyraclostrobin into the system. Ultrasonically vibrate for 20-30 minutes. Dissolve the prescribed amounts of polyhexamethylene biguanide, sodium dichloroisocyanurate, sodium nitrite, and dodecylbenzenesulfonic acid in water, and add the resulting solution dropwise to the ultrasonically vibrated system. Adjust the temperature to 25-30°C, and add the prescribed amounts of tartaric acid, calcium stearate, and disodium EDTA, stirring until well mixed.