Traditional Chinese medicine preparation and method for preventing and treating Huanglongbing of navel orange

Abstract

The invention discloses a traditional Chinese medicine preparation and method for preventing and treating navel orange liberobacter asiaticum. The prevention and treatment method comprises the following steps: a trunk infusion step: infusing a diluent of the traditional Chinese medicine preparation into xylem and / or phloem of a plant trunk; and a foliage spraying step: spraying a foliage fertilizer containing pyroligneous liquor to the foliage of the plant. The traditional Chinese medicine preparation comprises a Chinese herbal medicine extracting solution and aminated xylan. According to the method, a synergistic system of bacteriostasis, nutrition and immunological enhancement is formed through a comprehensive prevention and treatment method of trunk infusion and foliage spraying of the pyroligneous liquid foliage fertilizer. The traditional Chinese medicine preparation infused into the trunk inhibits pathogenic bacteria from the inside in a long-acting mode, the pyroligneous leaf fertilizer provides nutrition from the outside, regulates growth and induces system resistance, internal and external synergism is achieved, diseases are directly inhibited, the nutritional status of diseased trees is remarkably improved, the autoimmunity of the trees is enhanced, and both symptoms and root causes of the huanglongbing are treated.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant disease prevention and control, and particularly relates to a traditional Chinese medicine preparation and method for preventing and treating Huanglongbing of navel orange. BACKGROUND

[0002] Huanglongbing of navel orange is a devastating disease caused by Candidatus Liberibacter, which mainly parasitizes the phloem of plants, hinders nutrient transport, causes tree decline, fruit malformation, and eventually leads to the death of the whole plant. Currently, the main methods for preventing and treating Huanglongbing include cutting down diseased trees, controlling the transmission vector of psyllids, and using antibiotics for trunk injection. However, cutting down trees results in huge losses; controlling psyllids cannot eradicate the pathogen that has already invaded the tree; and long-term use of antibiotics can easily lead to problems such as drug resistance of the pathogen, drug residues, and environmental pollution.

[0003] In the prior art, attempts have also been made to use plant-derived extracts for prevention and treatment, which have attracted attention due to their environmental friendliness and low risk of developing drug resistance. However, when the active ingredients of plant-derived extracts are directly injected through the trunk, there are often problems of unstable prevention and treatment effect. In addition, diseased trees of Huanglongbing generally have problems such as malnutrition and low immunity, and simple bacteriostasis cannot achieve tree recovery and long-term control of the disease.

[0004] Regarding the prevention and treatment methods for Huanglongbing of navel orange, Chinese patent document CN 119157016 A (application number 202411124361.7) discloses a method for repairing citrus Huanglongbing trees, which uses a combination of trunk infusion and foliar spraying for the diseased trees. Trunk infusion: drilling a hole at a distance of 20-50 cm from the ground of the tree trunk to infuse liquid A, which comprises the following raw materials in parts by weight: 30-45 parts of 99.6% ampicillin 500 times solution, 15-25 parts of 20% amoxicillin 500 times solution, 15-22 parts of 0.1% triacontanol 1000 times solution, 10-18 parts of 23% copper oleate 600 times solution, 7-14 parts of 80% mancozeb 500 times solution, 5-10 parts of 19% calcium and magnesium trace water-soluble fertilizer 1000 times solution, and 100-200 parts of water. Foliar spraying: 12-18 days after each trunk infusion, foliar spraying of liquid B, which is an antibacterial drug. SUMMARY

[0005] The technical problem to be solved by the present application is the problem of unstable effect of Chinese herbal medicine preparations and the problem of easy generation of pesticide residues and drug resistance in chemical prevention and treatment methods for Huanglongbing of navel orange, and a Huanglongbing prevention and treatment preparation and method that integrates "bacteriostasis-nutrition-immune enhancement" are provided.

[0006] The technical solution for achieving the first objective of this invention is a traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing (HLB) in navel oranges, comprising a traditional Chinese medicine extract and aminoxylan; the traditional Chinese medicine extract is obtained by extracting traditional Chinese medicine raw materials including Phellodendron bark, Scutellaria baicalensis, Forsythia suspensa, Lonicera japonica, Sophora flavescens, Coptis chinensis, Galla chinensis, Angelica sinensis, Achyranthes bidentata, Bupleurum chinense, and Glycyrrhiza uralensis; the weight of the aminoxylan is 1%-7% of the weight of the traditional Chinese medicine extract.

[0007] The weight parts of each component in the herbal raw materials are as follows: 20-40 parts of Phellodendron bark, 20-40 parts of Scutellaria baicalensis, 20-40 parts of Forsythia suspensa, 20-40 parts of Lonicera japonica, 20-40 parts of Sophora flavescens, 20-40 parts of Coptis chinensis, 20-40 parts of Galla chinensis, 5-15 parts of Angelica sinensis, 5-15 parts of Achyranthes bidentata, 5-15 parts of Bupleurum chinense, and 5-15 parts of Glycyrrhiza uralensis.

[0008] The preparation method of the herbal extract is as follows: After the Chinese herbal raw materials are crushed, they are first extracted with water. The amount of deionized water added is 8-12 times the weight of the Chinese herbal raw materials. The extraction temperature is 80-90℃ and the extraction time is 2-4 hours. After filtration, the water extract and filter residue are obtained.

[0009] The filter residue is subjected to alcohol extraction, wherein an aqueous ethanol solution with a concentration of 65%-75% is used as the solvent, and the amount of the aqueous ethanol solution added is 8-12 times the weight of the herbal raw material. The extraction temperature is 60-70℃ and the extraction time is 1.5-2.5 hours. After filtration, the two extracts are combined and the alcohol is removed to obtain the herbal extract, which is 9-15 times the weight of the dry material.

[0010] In this process, aminoxylan is added to the herbal extract at a concentration of 1%-7% of the extract's weight. After stirring and homogenizing, the herbal preparation is obtained.

[0011] The traditional Chinese medicine preparation, after being diluted with water at a weight ratio of 1:(400-500), has a viscosity of 1.2-2.3 mPa·s at 25°C.

[0012] The technical solution for achieving the second objective of this invention is a method for controlling Huanglongbing (HLB) in navel oranges, comprising the following steps: Trunk infusion procedure: Infuse a diluted solution of the above-mentioned traditional Chinese medicine preparation into the xylem and / or phloem of the plant trunk; and Foliar spraying procedure: Spray the leaves of the plant with a foliar fertilizer containing wood vinegar.

[0013] The diluent of the traditional Chinese medicine preparation is obtained by mixing the traditional Chinese medicine preparation with water at a weight ratio of 1:(400-500); based on a single navel orange tree, the amount of dried traditional Chinese medicine material used per tree is 3g-15g.

[0014] The trunk infusion procedure is performed once a year, with each infusion lasting 3-10 days, and is carried out from March to June each year or after the spring shoots have matured.

[0015] The foliar spraying step is performed after the trunk infusion step, and after the first foliar spraying is completed, the foliar spraying step is repeated once a month.

[0016] In the trunk infusion step, at least two needles are used for infusion, including a first needle that penetrates into the xylem of the trunk and a second needle that penetrates the phloem of the trunk.

[0017] This invention has positive effects: This invention employs a comprehensive control method combining trunk infusion and foliar spraying of wood vinegar foliar fertilizer, forming a synergistic system of "antibacterial inhibition, nutrition, and immune enhancement." The herbal preparation infused into the trunk provides long-term internal inhibition of pathogens, while the wood vinegar foliar fertilizer provides external nutrition, regulates growth, and induces systemic resistance. This synergistic effect not only directly inhibits the disease but also significantly improves the nutritional status of diseased trees and enhances their own immunity, achieving a comprehensive cure for Huanglongbing (HLB).

[0018] The traditional Chinese medicine preparation infused into the tree trunk includes extracts of traditional Chinese medicine and aminoxylan. Aminoxylan has good biocompatibility, and its amino groups can bind with plant-derived active ingredients through intermolecular forces. After infusion into the tree, the active ingredients are slowly released, thereby maintaining a long-term effective antibacterial concentration in the phloem of the plant. This solves the problems of short duration of action and frequent application required by traditional plant-derived agents. Detailed Implementation

[0019] The following describes some of the possible embodiments of the present invention, intended to provide a basic understanding of the invention, and is not intended to identify the key or decisive elements of the invention or limit the scope of protection. It is readily understood that, based on the technical solutions of the present invention, those skilled in the art can propose other interchangeable implementations without altering the essential spirit of the invention. Therefore, the following specific embodiments are merely illustrative examples of the technical solutions of the present invention and should not be considered as the entirety of the invention or as limitations or restrictions on the technical solutions of the present invention.

[0020] (Example 1) The method for preventing and controlling Huanglongbing in navel oranges in this embodiment includes the following steps: ① Trunk infusion. On a sunny day in April, drill a hole in the trunk 40cm-60cm above the ground and infuse the trunk with the solution. Use two needles, one reaching deep into the xylem of the tree and the other penetrating the phloem. The two needles should be at equal or nearly equal heights from the ground and spaced evenly along the circumference of the trunk.

[0021] The trunk infusion uses diluted traditional Chinese medicine preparations, which include herbal extracts and aminoxylan.

[0022] The amount of dried medicinal herbs used per tree is 3g-15g (denoted as m). After processing, the dried medicinal herbs are used to obtain a medicinal herb extract. The extract is then mixed with aminoxylan to obtain a traditional Chinese medicine preparation. The weight of the aminoxylan is 1%-7% of the weight of the herb extract. The preparation is diluted with water at a ratio of 1:400-500 and applied to the trunk of the tree for treatment.

[0023] Each 1g of dried Chinese herbal medicine contains the following components by weight: 20-40 parts of Phellodendron bark, 20-40 parts of Scutellaria baicalensis, 20-40 parts of Forsythia suspensa, 20-40 parts of Lonicera japonica, 20-40 parts of Sophora flavescens, 20-40 parts of Coptis chinensis, 20-40 parts of Galla chinensis, 5-15 parts of Angelica sinensis, 5-15 parts of Achyranthes bidentata, 5-15 parts of Bupleurum chinense, and 5-15 parts of Glycyrrhiza uralensis.

[0024] The components of the above-mentioned traditional Chinese medicine work synergistically: Phellodendron bark, Coptis chinensis, and Sophora flavescens: rich in alkaloids such as berberine and matrine, which can destroy the cell membrane structure of Huanglongbing bacteria and inhibit the metabolism and reproduction of pathogens.

[0025] Honeysuckle, forsythia, and scutellaria: contain chlorogenic acid, forsythoside, baicalin, and other components, which enhance the antibacterial spectrum coverage and have a specific inhibitory effect on phloem bacilli.

[0026] Schisandra chinensis and Angelica sinensis contain tannins and flavonoids, which can astringe wounds, repair damaged plant tissues, and reduce the pathways for pathogen infection.

[0027] Achyranthes bidentata and Bupleurum chinense contain oleanolic acid and saikosaponin, which can clear the vascular bundles of plants, promote nutrient transport and metabolism, and enhance the tree's resistance to adverse conditions.

[0028] Licorice contains glycyrrhizin and flavonoids, which can harmonize the properties of various medicines, supplement amino acids and minerals, and enhance the plant's immunity.

[0029] The traditional Chinese medicine preparation in this embodiment is prepared according to the following method: S1. Weigh out 20 parts of Phellodendron bark, 20 parts of Scutellaria baicalensis, 30 parts of Forsythia suspensa, 30 parts of Lonicera japonica, 30 parts of Sophora flavescens, 20 parts of Coptis chinensis, 30 parts of Galla chinensis, 10 parts of Angelica sinensis, 10 parts of Achyranthes bidentata, 10 parts of Bupleurum chinense, and 5 parts of Glycyrrhiza uralensis according to their respective weights. Record the total weight as M1.

[0030] S2. Dry and pulverize the 11 Chinese herbal medicines weighed above to 50-60 mesh, add 10 times their weight (10M) of deionized water, extract at 85℃ for 3 hours, filter and take the filtrate, then add 10 times their weight of 70% ethanol solution of the Chinese herbal medicine raw materials to the filter residue, extract at 65℃ for 2 hours, filter, combine the two extracts, remove the ethanol under reduced pressure to obtain the Chinese herbal medicine extract.

[0031] S3. Add aminoxylan to the herbal extract obtained in step S2. The amount of aminoxylan added is 1%-7% of the weight of the herbal extract (2% in this example). Stir well to obtain the herbal preparation for later use. The total weight of the herbal preparation is denoted as M2.

[0032] For the Chinese medicine preparations obtained above, the weight of the Chinese medicine preparation used for each tree is m*M2 / M1.

[0033] In this embodiment, the amount of dried Chinese herbal medicine used per tree is 3g. If the weight of the Chinese herbal medicine is M1=300g, then the weight of the Chinese herbal medicine preparation used per tree is M2 / 100.

[0034] When administering intravenous infusion to the tree trunk, dilute the M2 / 100g traditional Chinese medicine preparation with water at a ratio of 1:400. Insert the infusion needle into a hole drilled 50cm above the ground in the trunk and inject the diluted medicine into the main trunk for treatment. The diluted medicine preparation is typically infused over 3-10 days, and the treatment is administered once a year.

[0035] The core principle of using intravenous infusion to control Huanglongbing (HLB) in navel orange trees is to utilize the transpiration pull of the plant's xylem and the passive water absorption effect of root pressure, combined with the external pressure of the infusion, to allow the medicine to enter the xylem of the trunk through a needle hole. The medicine then travels through the tree's water transport system to the branches, leaves, and fruits, achieving whole-plant conduction and absorption. When the herbal preparation is diluted with water at a ratio of 1:400-500, its viscosity at 25℃ is 1.2-2.3 mPa·s, at which point the medicine can easily penetrate the interior of the trunk.

[0036] ② Foliar spraying. Apply foliar fertilizer once while administering sap to the tree trunk, and then apply foliar fertilizer once a month thereafter.

[0037] The foliar fertilizer is a foliar fertilizer containing wood vinegar. The foliar fertilizer in this embodiment includes 60 parts wood vinegar, 10 parts urea, 1 part superphosphate, 2 parts zinc sulfate, 2 parts magnesium sulfate, 3 parts copper sulfate, 2 parts manganese sulfate, 1 part ferrous sulfate, 1 part complexing agent, 3 parts humic acid, and 10 parts compound amino acids.

[0038] Spray each tree with 15-45g of the above-mentioned foliar fertilizer per month. When using, dilute it with water at a ratio of 1:400-500 (by volume) before spraying.

[0039] Wood vinegar is obtained by separating and recombining the liquid components obtained by condensing the gaseous products produced by the dry distillation and pyrolysis of plants. Wood vinegar contains hundreds of organic compounds, among which small molecules such as phenols, organic acids, aldehydes, and ketones have good bactericidal and insecticidal effects.

[0040] The infusion solution used in the trunk is a slow-release traditional Chinese medicine preparation. After entering the xylem, it is slowly released along with the water transport in the tree and can form a continuous antibacterial environment. The effect can last for up to 12 months. On the other hand, the foliar spray is a fast-acting antibacterial agent with a short-lasting effect (about 1 month). Therefore, it needs to be applied once a month. The two form a "long-acting systemic + short-acting supplement" prevention and control combination.

[0041] (Experimental Example 1) In March 2025, a small-scale trial was conducted at the Xianke Navel Orange Orchard in Yudu County, Jiangxi Province. Seven infected navel orange trees of similar age and with similar levels of Huanglongbing (HLB) infection in the field were selected as experimental materials. Five of these trees were treated with an infusion treatment solution for HLB infection (a traditional Chinese medicine preparation prepared according to the method in Example 1, using 3 g of dried herbal material per tree, diluted with water at a ratio of 1:400), and were numbered Treatment 1, Treatment 2, Treatment 3, Treatment 4, and Treatment 5. The remaining two trees were not treated at all and served as a blank control, numbered Control 1 and Control 2. Throughout the experiment, the growth of the plants was observed, photographed, and recorded in detail.

[0042] Meanwhile, dynamic detection of Huanglongbing pathogen content in plant leaves was carried out. The leaf selection method was as follows: one tip branch was randomly selected from each experimental navel orange tree, and each branch had 5 leaves available for pathogen detection. The detection data results are shown in Table 1.

[0043] The pathogen was detected using quantitative real-time PCR. A 96-well real-time PCR instrument was used to quantitatively detect the Huanglongbing pathogen on the leaves of navel orange trees at three stages: before, during, and after the experiment.

[0044] Table 1 (Note: When the CT value is greater than 35, it is determined that the Huanglongbing pathogen has been completely eliminated, and the result is negative; / indicates that Huanglongbing was not detected.) Compared with control groups 1-2, PCR tests on Huanglongbing-affected trees treated with the method of this invention showed negative results for treatments 1, 3, and 5. The results demonstrate that the therapeutic agents and methods of this invention have a significant curative effect on Huanglongbing. The success rate reached 60% after 3 months.

[0045] (Experimental Example 2) In this experiment, each tree was treated with a traditional Chinese medicine preparation made from 8 g of dried Chinese herbal medicine (preparation method is the same as in Example 1); when applying the preparation, it was diluted with water at a ratio of 1:400, and then the solution was injected through a hole drilled at a height of 50 cm on the tree trunk.

[0046] In March 2025, a small-scale trial was conducted at the Xianke Navel Orange Orchard in Yudu County, Jiangxi Province. Five infected navel orange trees of similar age and with similar levels of Huanglongbing (HLB) infection in the field were selected as experimental materials and numbered sequentially as Treatment 6, Treatment 7, Treatment 8, Treatment 9, and Treatment 10. The experimental results are as follows: Table 2 (Note: When the CT value is greater than 35, it is determined that the pathogen in Huanglong has been completely eliminated, and the result is negative.) The results showed that the therapeutic agent and method of the present invention had a good therapeutic effect on navel orange trees with different degrees of disease. Three months after the end of treatment, all treated navel orange trees were tested for Huanglongbing (HLB) and the tree vigor was observed. The test results showed that the trees were growing well, the new leaves of the spring shoots were green, and there was no yellowing or mottling. No reinfection of navel oranges with HLB was found, and the success rate of treatment reached 80%.

[0047] (Experimental Example 3) In this experiment, each tree was treated with a traditional Chinese medicine preparation made from 15 g of dried Chinese herbal medicine (preparation method is the same as in Example 1); when applying the preparation, it was diluted with water at a ratio of 1:400, and then the solution was injected through a hole drilled 50 cm above the trunk.

[0048] In March 2025, a small-scale trial was conducted at the Xianke Navel Orange Orchard in Yudu County, Jiangxi Province. Five infected navel orange trees of similar age and with similar levels of Huanglongbing (HLB) infection in the field were selected as experimental materials and numbered sequentially as Treatment 11, Treatment 12, Treatment 13, Treatment 14, and Treatment 15. The experimental results are as follows: Table 3 (Note: When the CT value is greater than 35, it is determined that the pathogen in Huanglong has been completely eliminated, and the result is negative.) The results showed that the therapeutic agent and method of the present invention had a good therapeutic effect on severely diseased navel orange trees. The test results showed that the trees were growing well, all the new leaves of the spring shoots were green, and there was no yellowing or mottling. The success rate of treatment reached 80% three months after the end of treatment.

[0049] (Experimental Example 4) In this experiment, each tree was treated with a traditional Chinese medicine preparation made from 15 g of dried herbal material (preparation method as in Example 1). The preparation was diluted with water at a ratio of 1:400, and then injected through a hole drilled 50 cm above the tree trunk. Foliar fertilizer was also applied monthly. Each tree was sprayed with 25 g of foliar fertilizer diluted with water at a ratio of 1:500.

[0050] In March 2025, a small-scale trial was conducted at the Xianke Navel Orange Orchard in Yudu County, Jiangxi Province. Five infected navel orange trees of similar age and with similar levels of Huanglongbing (HLB) infection in the field were selected as experimental materials and numbered sequentially as Treatment 16, Treatment 17, Treatment 18, Treatment 19, and Treatment 20. The experimental results are as follows: Table 4 (Note: When the CT value is greater than 35, it is determined that the Huanglong pathogen has been completely eliminated, and the result is negative.) The test results showed that the trees were growing well, and all the new leaves of the spring shoots were green, without yellowing or mottling. Spraying with wood vinegar was significantly beneficial to the treatment of Huanglongbing (HLB). After one month, the success rate of HLB treatment reached 80%, which was faster than the method of infusion of pure Chinese herbal medicine into the tree trunk. This indicates that the combined use of Chinese medicine preparations and wood vinegar is beneficial to the faster treatment of HLB.

[0051] (Experimental Example 5) In March 2025, a small-scale trial was conducted at the Xianke Navel Orange Orchard in Yudu County, Jiangxi Province. Ten infected navel orange trees of similar age and with similar levels of Huanglongbing (HLB) infection in the field were selected as experimental materials. Five trees were infused with a diluted traditional Chinese medicine preparation from Example 1, numbered Treatment 21, Treatment 22, Treatment 23, Treatment 24, and Treatment 25. The other five trees were infused with a diluted traditional Chinese medicine preparation without added aminoxylan, numbered Control 3, Control 4, Control 5, Control 6, and Control 7. Each tree used 15 g of the traditional Chinese medicine preparation made from dried herbs (preparation method as in Example 1). The preparation was diluted with water at a ratio of 1:400, and then infused through a hole drilled 50 cm above the trunk. Foliar fertilizer was also applied monthly. Each tree was sprayed with 35 g of foliar fertilizer diluted with water at a ratio of 1:500.

[0052] Table 5 The test results showed that the treated trees grew well, with all new leaves on the spring shoots being green, and no yellowing or mottling was observed. After three months of treatment with a diluted traditional Chinese medicine preparation containing aminoxylan, the success rate of Huanglongbing control was significantly higher than that of treatment with only traditional Chinese medicine trunk infusion without aminoxylan. This indicates that aminoxylan can promote the slow release of the active ingredients in the traditional Chinese medicine preparation, thereby achieving a sustained therapeutic effect on Huanglongbing.

Claims

1. A traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing (HLB) in navel oranges, characterized in that, It includes a traditional Chinese medicine extract and aminoxylan; the traditional Chinese medicine extract is obtained by extracting raw materials containing traditional Chinese medicines such as Phellodendron bark, Scutellaria baicalensis, Forsythia suspensa, Lonicera japonica, Sophora flavescens, Coptis chinensis, Galla chinensis, Angelica sinensis, Achyranthes bidentata, Bupleurum chinense and Glycyrrhiza uralensis; the weight of the aminoxylan is 1%-7% of the weight of the traditional Chinese medicine extract.

2. The traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing in navel oranges according to claim 1, characterized in that: The weight parts of each component in the herbal raw materials are as follows: 20-40 parts of Phellodendron bark, 20-40 parts of Scutellaria baicalensis, 20-40 parts of Forsythia suspensa, 20-40 parts of Lonicera japonica, 20-40 parts of Sophora flavescens, 20-40 parts of Coptis chinensis, 20-40 parts of Galla chinensis, 5-15 parts of Angelica sinensis, 5-15 parts of Achyranthes bidentata, 5-15 parts of Bupleurum chinense, and 5-15 parts of Glycyrrhiza uralensis.

3. The traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing in navel oranges according to claim 2, characterized in that... The preparation method of the herbal extract is as follows: After the Chinese herbal raw materials are crushed, they are first extracted with water. The amount of deionized water added is 8-12 times the weight of the Chinese herbal raw materials. The extraction temperature is 80-90℃ and the extraction time is 2-4 hours. After filtration, the water extract and filter residue are obtained. The filter residue is subjected to alcohol extraction, wherein an aqueous ethanol solution with a concentration of 65%-75% is used as the solvent, the amount of the aqueous ethanol solution added is 8-12 times the weight of the Chinese herbal medicine raw material, the extraction temperature is 60-70℃, and the extraction time is 1.5-2.5 hours; after filtration, the two extracts are combined and the alcohol is removed to obtain the Chinese herbal medicine extract.

4. The traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing in navel oranges according to claim 3, characterized in that: Aminated xylan is added to the herbal extract at a rate of 1%-7% of the extract's weight. After stirring evenly, a traditional Chinese medicine preparation is obtained.

5. The traditional Chinese medicine preparation for the prevention and treatment of Huanglongbing in navel oranges according to claim 1, characterized in that: The traditional Chinese medicine preparation, after being diluted with water at a weight ratio of 1:(400-500), has a viscosity of 1.2-2.3 mPa·s at 25°C.

6. A method for controlling Huanglongbing (HLB) in navel oranges, characterized in that... Includes the following steps: Tree trunk infusion procedure: Infuse the diluted solution of the traditional Chinese medicine preparation described in claim 1 into the xylem and / or phloem of the plant trunk; as well as Foliar spraying procedure: Spray the leaves of the plant with a foliar fertilizer containing wood vinegar.

7. The method for controlling Huanglongbing in navel oranges according to claim 6, characterized in that: The diluent of the traditional Chinese medicine preparation is obtained by mixing the traditional Chinese medicine preparation with water at a weight ratio of 1:(400-500); based on a single navel orange tree, the amount of dried traditional Chinese medicine material used per tree is 3g-15g.

8. The method for controlling Huanglongbing in navel oranges according to claim 6, characterized in that: The trunk infusion procedure is performed once a year, with each infusion lasting 3-10 days, and is carried out from March to August each year.

9. The method for controlling Huanglongbing in navel oranges according to claim 6, characterized in that: The foliar spraying step is performed after the trunk infusion step, and after the first foliar spraying is completed, the foliar spraying step is repeated once a month.

10. The method for controlling Huanglongbing in navel oranges according to claim 6, characterized in that: In the trunk infusion step, at least two needles are used for infusion, including a first needle that penetrates into the xylem of the trunk and a second needle that penetrates the phloem of the trunk.

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