Linseed oil for purifying heart and cerebral vessels, preparation method, vegetable oil product and application

Abstract

The invention provides linseed oil for purifying heart and cerebral vessels, a preparation method, a vegetable oil product and application, and relates to the technical field of food. The preparation method comprises the following steps: (1) carrying out crushing, segmented heating, steaming and frying and pulping treatment on flaxseeds to obtain flaxseed pulp; (2) extracting the flaxseed slurry with water, ethanol and normal hexane in sequence to obtain an extraction mixture A; (3) mixing and extracting the extraction mixture A and a deep eutectic solvent, and taking an upper layer to obtain an extraction mixture B; the eutectic solvent is a mixture of choline chloride and n-butyl alcohol; and (4) extracting the mixture B under reduced pressure to remove the solvent, and carrying out post-treatment to obtain the linseed oil. According to the preparation method, the yield of the linseed oil can be increased, the acid value and the peroxide value are reduced, and the linseed oil has excellent effects of reducing blood pressure, reducing blood fat, maintaining the healthy level of blood pressure and maintaining the healthy level of blood fat, and can be applied to preparation of vegetable oil with the effect of purifying heart and cerebral vessels.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of food, and particularly relates to a cardiovascular and cerebrovascular purifying flaxseed oil, a preparation method thereof, a vegetable oil product and application. BACKGROUND

[0002] Cardiovascular and cerebrovascular diseases mainly include coronary heart disease, hypertension, hyperlipidemia, stroke, etc. In the process of preventing and treating cardiovascular diseases, dietary intervention is considered as one of the basic and effective strategies. Oil is an important component of daily diet, and its type and intake proportion have a significant impact on cardiovascular health. At present, there are various edible oils on the market, but many traditional vegetable oils have certain limitations in fatty acid composition, such as high content of saturated fatty acids, lack of omega-3 polyunsaturated fatty acids (PUFA) or poor stability, etc., which are difficult to efficiently maintain the healthy level of blood pressure / blood lipid.

[0003] Linum is a genus of the Linaceae family, and is an annual or perennial herb. Flax is an important oil crop, and the mass fraction of linolenic acid in flax is as high as 40%-60%. Flaxseed oil is a kind of nutrient-rich vegetable oil extracted from flax seeds, which has various nutritional ingredients, including omega-3 fatty acids, omega-6 fatty acids and other beneficial substances. Flaxseed oil is rich in alpha-linolenic acid, which belongs to omega-3 series polyunsaturated fatty acids, and has various biological activities beneficial to cardiovascular health, such as anti-inflammatory, hypolipidemic and anti-arrhythmic effects.

[0004] Flaxseed oil is generally prepared by pressing, but the oil quality and yield are affected due to improper treatment in the process of drying or high-temperature roasting. In oilseeds, oil exists in the form of lipoprotein, lipopolysaccharide and other complexes. In order to improve the oil yield, the cell wall needs to be destroyed, but after high-temperature treatment, although the oil yield of flaxseed is improved, the peroxide value and acid value of flaxseed oil change greatly, and the stability is poor.

[0005] Chinese invention patent CN120754158A discloses a flax olive oil for improving the prevention and treatment of cardiovascular diseases, belonging to the technical field of oil and fat. The raw material components mainly include olive oil and flaxseed oil, wherein the mass ratio of olive oil to flaxseed oil is 1.5-2.5:1. The flaxseed oil is obtained by screening, removing impurities and abnormal particles, then crushing, multiple extractions with a mixed extractant, degumming, bleaching and deodorizing. The extraction temperature is 20-25℃, the liquid-solid ratio is 9-10:1 mL / g, the single extraction time is 35-45 min, and the extraction tank pressure is 0.30-0.40 MPa. The mixed extractant includes 18.8-37.7% ethanol, 29.7-30.4% n-butane, 19.7-38.5% dimethyl ether and 12.4-12.9% water by mass fraction. However, the acid value and peroxide value of the flaxseed oil prepared by the technical solution of the invention are relatively high, and the quality still needs to be improved.

[0006] Chinese invention patent CN108546586A discloses a flax oil refining method for protecting linolenic acid, including the following steps: step one, pressing flaxseed; step two, alkali refining and deacidification; step three, water hydration degumming of flaxseed oil; step four, deodorization of flaxseed oil. The materials required for making flax oil are as follows: flaxseed, diethyl ether, ethanol and n-hexane. The whole production process of the flax oil refining method is in a low-temperature environment, which avoids the damage to the α-linolenic acid structure component in the unsaturated fatty acid in flax oil caused by high temperature, protects the α-linolenic acid, and avoids the loss of nutritional substances of α-linolenic acid. However, the oil extraction effect of flax oil is limited, and the yield is low.

[0007] In addition, the functional substances of single plant oil are relatively single compared with the functional components of compound oil, which cannot achieve the effect of significantly improving the heart and blood vessels. The current research on plant oil is still focused on blended oil. For example, Chinese invention patent CN108041184A discloses a mixed oil for preventing and treating cardiovascular diseases, which is composed of the following raw materials in parts by weight: fish oil 1-10 parts, perilla oil 1-10 parts, pomegranate seed oil 1-10 parts, vegetable oil 12-80 parts, stabilizer 0.9-1.5 parts and flavor additive 0.4-0.6 parts. The weight ratio of EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) in the fish oil is 6-9:1, and the weight of EPA and DHA accounts for 50-95% in the fish oil. The mixed oil of the invention contains EPA, flavonoids, vitamin D, sterols, punicic acid, phytosterols, etc., which has a certain curative effect on the heart and blood vessels. However, the curative effect mainly depends on the weight ratio of EPA and DHA, which is not directly given. The mutual cooperation of various plant oils can bring synergistic effects of reducing blood pressure, reducing blood lipids and improving blood vessel dilation function. SUMMARY

[0008] The present application provides a kind of purification cardiovascular and cerebrovascular flaxseed oil and preparation method, vegetable oil product and application, the preparation method of flaxseed oil is researched, the yield, purity and the content of omega-3 such as effective component of flaxseed oil are improved;With the prepared flaxseed oil as one of the components, the preparation of vegetable oil product is carried out, and the obtained vegetable oil product has excellent effects of reducing blood pressure, reducing blood lipid, improving blood vessels, maintaining blood pressure health level and maintaining blood lipid health level.

[0009] To achieve the above object, the technical scheme adopted by the present application is as follows: Firstly, the present application provides a kind of preparation method of flaxseed oil capable of purifying cardiovascular and cerebrovascular, comprising the following steps: (1) flaxseed is crushed, sectionally heated and steamed, and ground pulp treatment is carried out to obtain flaxseed slurry; (2) the flaxseed slurry is sequentially extracted with water, ethanol and n-hexane to obtain an extraction mixture A; (3) the extraction mixture A and the eutectic solvent are mixed and extracted, and the upper layer is taken to obtain an extraction mixture B; the eutectic solvent is a mixture of choline chloride and n-butanol; (4) the extraction mixture B is subjected to a post-treatment step of solvent removal under reduced pressure, standing, degumming, decolorization and deodorization to obtain flaxseed oil.

[0010] Preferably, in step (1), the crushing is a conventional crushing treatment, and the particle size is 0.2-3 mm.

[0011] Preferably, in step (1), the sectionally heated and steamed treatment is specifically set as follows: under a wet state, steaming and frying at 60-80℃ for 120-240s, then heating to 90-110℃ for steaming and frying for 30-90s, and finally heating to 120-130℃ for steaming and frying for 20-40s.

[0012] Further preferably, in step (1), the sectionally heated and steamed treatment is specifically set as follows: under a wet state, steaming and frying at 70℃ for 180s, then heating to 100℃ for steaming and frying for 60s, and finally heating to 125℃ for steaming and frying for 30s.

[0013] Preferably, in step (1), the grinding treatment is specifically as follows: using a stone mill at 20-30 rpm for grinding until the flaxseed is broken open without residue.

[0014] Preferably, step (2) is specifically as follows: the flaxseed slurry and water at 80-100℃ are mixed in batches, and the oil layer is obtained by standing and layering; the oil layer is mixed with ethanol and n-hexane, and stirred extraction is carried out at 50-65℃ for 20-40 min to obtain the extraction mixture A.

[0015] Further preferably, step (2) is specifically: mixing the flaxseed slurry and water in several times, with the water amount gradually decreasing each time, and then standing to separate into an oil layer; mixing the oil layer with ethanol and n-hexane, and then extracting at 55°C for 30 min to obtain an extraction mixture A.

[0016] Further preferably, in step (2), the mixing in several times is 2-4 times, with the water amount gradually decreasing each time; more preferably, in step (2), the mixing in several times is 3 times, with the water amount being 45-60% (wt%) of the total water amount in the first time, 30-40% (wt%) of the total water amount in the second time, and 10-15% (wt%) of the total water amount in the third time, and each time is stirred for 3-8 min.

[0017] More preferably, in step (2), the mixing in several times is 3 times, with the water amount being 50% (wt%) of the total water amount in the first time, 35% (wt%) of the total water amount in the second time, and 15% (wt%) of the total water amount in the third time, and each time is stirred for 5 min.

[0018] Preferably, in step (2), the total water amount is 10-20 times the mass of the flaxseed; the volume ratio of ethanol, n-hexane and water is 5-15:10-20:50-100; and the water refers to the total water amount used in this step.

[0019] Further preferably, in step (2), the total water amount is 15 times the mass of the flaxseed; the volume ratio of ethanol, n-hexane and water is 10:15:75.

[0020] Preferably, in step (3), the molar ratio of choline chloride and n-butanol is 1:3-4; further preferably, in step (3), the molar ratio of choline chloride and n-butanol is 1:3.5.

[0021] Preferably, in step (3), the volume ratio of the extraction mixture A and the deep eutectic solvent is 1:3-5.

[0022] Further preferably, in step (3), the volume ratio of the extraction mixture A and the deep eutectic solvent is 1:4.

[0023] Preferably, in step (3), the temperature of the mixing extraction is 30-40°C, and the time is 2-4 h. Further preferably, in step (3), the temperature of the mixing extraction is 37°C, and the time is 3 h.

[0024] Preferably, in step (4), the standing time is 4-6 h, and the precipitate is removed; further preferably, the step of removing the precipitate is not limited to centrifugation or filtration, and the standing time is 5 h.

[0025] Preferably, in step (4), the degumming, bleaching, deodorizing treatment is a conventional treatment method in the art, not limited to hydration degumming, alkali refining deacidification, and stripping deodorization.

[0026] In some embodiments of the present application, the degumming, bleaching, and deodorizing post-processing steps refer to the methods disclosed in Publication No. CN108546586A or Publication No. CN120754158A.

[0027] In some embodiments of the present application, the degumming, bleaching, and deodorizing post-processing steps refer to the steps in Example 1 of Publication No. CN120754158A.

[0028] Then, the present application provides a flaxseed oil capable of purifying cardiovascular and cerebrovascular, prepared by the above preparation method.

[0029] Furthermore, the present application provides the use of the above flaxseed oil in preparing plant oil with the function of purifying cardiovascular and cerebrovascular.

[0030] Preferably, in the use, the plant oil with the function of purifying cardiovascular and cerebrovascular is not limited to plant oil capable of maintaining healthy blood pressure and maintaining healthy blood lipid.

[0031] Finally, the present application provides a plant oil product capable of purifying cardiovascular and cerebrovascular, which comprises, by weight, 40-50 parts of sunflower seed oil, 40-50 parts of the above flaxseed oil, 0.5-1.5 parts of safflower seed oil, 0.5-1.5 parts of walnut oil, 0.5-1.5 parts of pumpkin seed oil, 0.5-1.5 parts of milk thistle seed oil, 0.5-1.5 parts of perilla seed oil, and 0.5-1.5 parts of olive oil.

[0032] Preferably, the plant oil product comprises, by weight, 47 parts of sunflower seed oil, 47 parts of the above flaxseed oil, 1 part of safflower seed oil, 1 part of walnut oil, 1 part of pumpkin seed oil, 1 part of milk thistle seed oil, 1 part of perilla seed oil, and 1 part of olive oil.

[0033] In the present application, the components are uniformly mixed to obtain a plant oil product.

[0034] Compared with the prior art, the present application has the following beneficial effects: 1. In the preparation method of the flaxseed oil of the present application, the flaxseed oil obtained by the step of segmented temperature increase steaming and frying under specific conditions, pulp grinding, water and organic solvent extraction, and eutectic solvent extraction treatment has high oil yield and low peroxide value and acid value, and the quality and yield of the flaxseed oil are significantly improved. In addition, the flaxseed oil prepared by the specific method has excellent effects of lowering blood pressure, lowering blood lipid, maintaining healthy blood pressure, maintaining healthy blood lipid, and improving vasodilation function.

[0035] 2. The flaxseed oil of the present application is used in combination with sunflower oil, safflower oil, walnut oil, pumpkin seed oil, milk thistle seed oil, perilla seed oil, and olive oil to obtain a plant oil product that has a more optimal effect of reducing TC and TG and increasing thromboxane B2 than conventional components of the prior art, and exhibits excellent functions of maintaining blood pressure at a healthy level, maintaining blood lipids at a healthy level, and improving vasodilation. DETAILED DESCRIPTION

[0036] The following non-limiting examples can provide a more complete understanding of the application to those of ordinary skill in the art, but are not intended in any way to limit the scope of the application. The following merely illustrates the scope of the application claimed, and those skilled in the art can make various changes and modifications to the application disclosed based on the disclosure, which should also be within the scope of the application claimed.

[0037] When the examples give numerical ranges, it should be understood that, unless the application indicates otherwise, each numerical range has two endpoints and any number between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the application have the same meaning as generally understood by those skilled in the art to which the application belongs.

[0038] The application will be further described below in the form of specific examples. The various chemical reagents used in the examples of the application are obtained through conventional commercial channels unless otherwise specified.

[0039] In the following examples, the safflower oil, sunflower oil, walnut oil, pumpkin seed oil, milk thistle seed oil, perilla seed oil, olive oil, maple oil, and flaxseed oil (commercially available) were purchased from Shanxi Xinjinshang Biological Technology Co., Ltd. The products from different manufacturers do not have a significant effect on the results.

[0040] In the following specific embodiments of the application, the post-treatment steps of degumming, bleaching, and deodorization are methods commonly used in the art, and are specifically referred to the steps in Example 1 of CN120754158A.

[0041] Example 1 A method for preparing flaxseed oil capable of purifying cardiovascular and cerebrovascular diseases, comprising the following steps: (1) The flaxseed is crushed to between 0.2-3 mm, then water is added to keep the flaxseed in a wet state; steaming and frying at 70°C for 180s, then increasing the temperature to 100°C for steaming and frying for 60s, and finally increasing the temperature to 125°C for steaming and frying for 30s; using a stone mill at 25 rpm for grinding until the flaxseed is broken and there is no residue; obtaining flaxseed slurry; (2) The linseed slurry is mixed with water at 90-95℃ for three times. The amount of water used in the first mixing is 50wt% of the total amount of water, the amount of water used in the second mixing is 35wt% of the total amount of water, and the amount of water used in the third mixing is 15wt% of the total amount of water. Each mixing is stirred for 5 minutes, and the oil layer is obtained by standing and separating. The total amount of water is 15 times the mass of the linseed; The oil layer is mixed with ethanol and n-hexane, and extracted at 55℃ for 30 minutes to obtain an extraction mixture A. In step (2), the volume ratio of ethanol, n-hexane and water is 10:15:75. (3) The extraction mixture A is mixed with 4 times the volume of a eutectic solvent at 37℃ for 3 hours, and the upper layer is obtained by standing to obtain an extraction mixture B. The eutectic solvent is a mixture of choline chloride and n-butanol at a molar ratio of 1:3.5. (4) The extraction mixture B is subjected to a post-processing step of standing for 5 hours, degumming, decolorization and deodorization to obtain linseed oil.

[0042] Example 2 A method for preparing linseed oil capable of purifying cardiovascular and cerebrovascular vessels, the steps are as follows: (1) The linseed is crushed to 0.2-3mm, and then water is added to keep the linseed in a wet state. It is steamed and fried at 60℃ for 240s, then the temperature is raised to 90℃ for steaming and frying for 90s, and finally the temperature is raised to 130℃ for steaming and frying for 20s. A stone mill is used for grinding at 20rpm until it is broken open without residue. A linseed slurry is obtained. (2) The same as in Example 1, an extraction mixture A is obtained. (3) The extraction mixture A is mixed with 5 times the volume of a eutectic solvent at 30℃ for 4 hours, and the upper layer is obtained by standing to obtain an extraction mixture B. The eutectic solvent is a mixture of choline chloride and n-butanol at a molar ratio of 1:3. (4) The extraction mixture B is subjected to a post-processing step of standing for 4 hours, degumming, decolorization and deodorization to obtain linseed oil.

[0043] Example 3 A method for preparing linseed oil capable of purifying cardiovascular and cerebrovascular vessels, the steps are as follows: (1) The linseed is crushed to 0.2-3mm, and then water is added to keep the linseed in a wet state. It is steamed and fried at 80℃ for 120s, then the temperature is raised to 110℃ for steaming and frying for 30s, and finally the temperature is raised to 120℃ for steaming and frying for 40s. A stone mill is used for grinding at 30rpm until it is broken open without residue. A linseed slurry is obtained. (2) The same as in Example 1, an extraction mixture A is obtained. (3) mixing the extraction mixture A and 3 times volume of the eutectic solvent at 40℃ for 2h, and taking the upper layer after standing to obtain extraction mixture B; the eutectic solvent is a mixture of choline chloride and n-butanol with a molar ratio of 1:4; (4) the extraction mixture B is subjected to a post-processing step of standing for 6h, degumming, decoloring and deodorizing to obtain the flaxseed oil.

[0044] Example 4 A method for preparing flaxseed oil capable of purifying cardiovascular and cerebrovascular system, and compared with Example 1, the step (2) is different, and the steps are as follows: (1) the flaxseed is crushed to 0.2-3mm, and then water is added to keep the flaxseed in a wet state; steaming and frying at 70℃ for 180s, then heating to 100℃ for steaming and frying for 60s, and finally heating to 125℃ for steaming and frying for 30s; using a stone mill at 25rpm for grinding until the flaxseed is broken and there is no residue; flaxseed slurry is obtained; (2) the flaxseed slurry is mixed with water at 80-90℃ for 3 times, the water amount in the first mixing is 45wt% of the total water amount, the water amount in the second mixing is 40wt% of the total water amount, and the water amount in the third mixing is 15wt% of the total water amount, each mixing is fully stirred for 8min, and the oil layer is obtained after standing and layering; the total water amount is 20 times of the mass of the flaxseed; the oil layer is mixed with ethanol and n-hexane, and extracted at 50℃ for 40min to obtain extraction mixture A; in step (2), the volume ratio of ethanol, n-hexane and water is 15:20:100; steps (3)-(4) are the same as those in Example 1.

[0045] Example 5 A method for preparing flaxseed oil capable of purifying cardiovascular and cerebrovascular system, and compared with Example 1, the step (2) is different, and the steps are as follows: (1) the flaxseed is crushed to 0.2-3mm, and then water is added to keep the flaxseed in a wet state; steaming and frying at 70℃ for 180s, then heating to 100℃ for steaming and frying for 60s, and finally heating to 125℃ for steaming and frying for 30s; using a stone mill at 25rpm for grinding until the flaxseed is broken and there is no residue; flaxseed slurry is obtained; (2) the flaxseed slurry is mixed with water at 95-100℃ for 3 times, the water amount in the first mixing is 60wt% of the total water amount, the water amount in the second mixing is 30wt% of the total water amount, and the water amount in the third mixing is 10wt% of the total water amount, each mixing is fully stirred for 3min, and the oil layer is obtained after standing and layering; the total water amount is 10 times of the mass of the flaxseed; the oil layer is mixed with ethanol and n-hexane, and extracted at 65℃ for 20min to obtain extraction mixture A; In step (2), the volume ratio of ethanol, n-hexane and water is 5:10:50; Steps (3)-(4) are the same as in Example 1.

[0046] Comparative Example 1 Different from Example 1, step (1) is replaced by a conventional steaming and frying, and pressing treatment, specifically: Step (1), flaxseed is broken to 0.2-3 mm, and is placed in a steamer to steam at a temperature of 45°C for 180 s, and is fried at a temperature of 58-60°C for 90 s, and the fried material is placed in a common press to press for 1 min at room temperature to obtain low-temperature crude oil (flaxseed slurry); Steps (2)-(4) are the same as in Example 1.

[0047] Comparative Example 2 Different from Example 1, the segmented temperature rising steaming and frying of step (1) is replaced by water-free frying, specifically: (1) flaxseed is broken to 0.2-3 mm, and is fried at a temperature of 125°C for 270 s without water; and is ground by a stone mill at 25 rpm until the flaxseed is broken and no residue is left to obtain flaxseed slurry; Steps (2)-(4) are the same as in Example 1.

[0048] Comparative Example 3 Different from Example 1, the treatment method of step (2) is different, and the flaxseed slurry is treated after mixing of ethanol, n-hexane and water, specifically: Step (1) is the same as in Example 1; (2) the flaxseed slurry is extracted with mixed solution A at 55°C for 30 min, the volume ratio of ethanol, n-hexane and water in mixed solution A is 10:15:75, and the amount of water is 15 times the mass of flaxseed; and an extraction mixture A is obtained; Steps (3)-(4) are the same as in Example 1.

[0049] Comparative Example 4 Different from Example 1, in step (2), the volume ratio of ethanol, n-hexane and water in mixed solution A is different. In this comparative example, the volume ratio of ethanol, n-hexane and water is 10:15:3, and the amount of water is the same as the mass of flaxseed; specifically: Step (1) is the same as in Example 1; (2) the flaxseed slurry is mixed with water at 90-95°C for 3 times, the amount of water in the first mixing is 50 wt% of the total amount of water, the amount of water in the second mixing is 35 wt% of the total amount of water, and the amount of water in the third mixing is 15 wt% of the total amount of water, each mixing is stirred for 5 min, and the oil layer is obtained by standing and separating; the total amount of water is the same as the mass of flaxseed; The oil layer and ethanol, n-hexane are mixed, and extracted at 55°C for 30 min to obtain an extraction mixture A; In step (2), the volume ratio of ethanol, n-hexane and water is 10:15:3; Steps (3)-(4) are the same as in Example 1.

[0050] Comparative Example 5 Different from Example 1, the n-hexane in step (2) is replaced by n-butane.

[0051] Step (1) is the same as in Example 1; (2) The flaxseed slurry and water at 90-95°C are mixed three times, the water amount in the first mixing is 50wt% of the total water amount, the water amount in the second mixing is 35wt% of the total water amount, and the water amount in the third mixing is 15wt% of the total water amount, each mixing is stirred for 5 min, and the oil layer is obtained by standing and layering; the total water amount is 15 times the mass of the flaxseed; The oil layer and ethanol, n-butane are mixed, and extracted at 55°C for 30 min to obtain an extraction mixture A; In step (2), the volume ratio of ethanol, n-butane and water is 10:15:75; Steps (3)-(4) are the same as in Example 1.

[0052] Comparative Example 6 Different from Example 1, the ethanol in step (2) is replaced by n-hexane.

[0053] Step (1) is the same as in Example 1; (2) The flaxseed slurry and water at 90-95°C are mixed three times, the water amount in the first mixing is 50wt% of the total water amount, the water amount in the second mixing is 35wt% of the total water amount, and the water amount in the third mixing is 15wt% of the total water amount, each mixing is stirred for 5 min, and the oil layer is obtained by standing and layering; the total water amount is 15 times the mass of the flaxseed; The oil layer and n-hexane are mixed, and extracted at 55°C for 30 min to obtain an extraction mixture A; In step (2), the volume ratio of n-hexane and water is 25:75; Steps (3)-(4) are the same as in Example 1.

[0054] Comparative Example 7 Different from Example 1, step (2) of Example 1 is omitted.

[0055] Step (1) is the same as in Example 1; (2) The flaxseed slurry is mixed with 4 times the volume of a deep eutectic solvent, and mixed at 37°C for 3h. The upper layer is taken after standing, to obtain an extraction mixture B; the deep eutectic solvent is a mixture of choline chloride and n-butanol in a molar ratio of 1:3.5; (3) The extraction mixture B is subjected to a post-processing step of standing for 5h, degumming, decoloring, and deodorizing, to obtain flaxseed oil.

[0056] Comparative Example 8 Unlike Example 1, step (3) of Example 1 is omitted.

[0057] Steps (1)-(2) are the same as in Example 1. (3) The extraction mixture A is subjected to a post-processing step of standing for 5h, degumming, decoloring, and deodorizing, to obtain flaxseed oil.

[0058] The flaxseed oil prepared in Example 1 is used in the preparation of the plant oil product in the following Example 6-Example 8, Comparative Example 8, and Comparative Example 10.

[0059] Example 6 A plant oil product capable of purifying cardiovascular and cerebrovascular blood vessels, the components of which are as follows in terms of weight parts: 47 parts of sunflower seed oil, 47 parts of flaxseed oil (Example 1), 1 part of safflower seed oil, 1 part of walnut oil, 1 part of pumpkin seed oil, 1 part of milk thistle seed oil, 1 part of perilla seed oil, and 1 part of olive oil.

[0060] The components are mixed uniformly to obtain the plant oil product.

[0061] Example 7 A plant oil product capable of purifying cardiovascular and cerebrovascular blood vessels, the components of which are as follows in terms of weight parts: 40 parts of sunflower seed oil, 50 parts of flaxseed oil (Example 1), 0.5 parts of safflower seed oil, 0.5 parts of walnut oil, 0.5 parts of pumpkin seed oil, 0.5 parts of milk thistle seed oil, 0.5 parts of perilla seed oil, and 0.5 parts of olive oil.

[0062] The components are mixed uniformly to obtain the plant oil product.

[0063] Example 8 A plant oil product capable of purifying cardiovascular and cerebrovascular blood vessels, the components of which are as follows in terms of weight parts: 50 parts of sunflower seed oil, 40 parts of flaxseed oil (Example 1), 1.5 parts of safflower seed oil, 1.5 parts of walnut oil, 1.5 parts of pumpkin seed oil, 1.5 parts of milk thistle seed oil, 1.5 parts of perilla seed oil, and 1.5 parts of olive oil.

[0064] The components are mixed uniformly to obtain the plant oil product.

[0065] Comparative Example 9 Different from example 6, the flaxseed oil (example 1) is replaced by flaxseed oil (commercially available).

[0066] A plant oil product capable of purifying cardiovascular and cerebrovascular, the components are as follows in terms of weight parts: 47 parts of sunflower seed oil, 47 parts of flaxseed oil (commercially available), 1 part of safflower seed oil, 1 part of walnut oil, 1 part of pumpkin seed oil, 1 part of milk thistle seed oil, 1 part of perilla seed oil, 1 part of olive oil.

[0067] The components are mixed uniformly to obtain the plant oil product.

[0068] Comparative example 10 Different from example 6, the sunflower seed oil is replaced by king's delight oil.

[0069] A plant oil product capable of purifying cardiovascular and cerebrovascular, the components are as follows in terms of weight parts: 47 parts of king's delight oil, 47 parts of flaxseed oil (example 1), 1 part of safflower seed oil, 1 part of walnut oil, 1 part of pumpkin seed oil, 1 part of milk thistle seed oil, 1 part of perilla seed oil, 1 part of olive oil.

[0070] The components are mixed uniformly to obtain the plant oil product.

[0071] Test 1: performance detection of flaxseed oil The oil yield of flaxseed oil (%) = mass of flaxseed oil / mass of flaxseed raw material x 100%.

[0072] Acid value: determination according to GB5009.229-2016; Peroxide value: determination according to GB5009.227-2016.

[0073] Parallel experiments were conducted for 3 times, and the results of flaxseed oil, acid value and peroxide value are shown in table 1.

[0074] Table 1

[0075] In table 1, compared with example 1, # P<0.05, ## P<0.01, indicating that the technical effects of the two values have significant differences.

[0076] As can be seen from table 1, by segmenting the flaxseed to be heated and fried, water and organic solvent step extraction, and eutectic solvent extraction treatment, the oil yield of flaxseed oil can be significantly improved, and the oxidation value and acid value can be reduced, thereby improving the quality of flaxseed oil.

[0077] Test 2: animal experiment 1. Experimental animals and modeling: 180-220g male Wistar rats (SPF level) after adaptive feeding, randomly selected 8 as normal control group, given 12% soybean oil in ordinary feed; the remaining 180 rats were fasted for 12 h, with 10% chloral hydrate intraperitoneal injection anesthesia, double kidney aortic stenosis operation was performed, so that the stenosis was 3 / 4, to cause the experimental hypertension model of rats. After 1 week of operation, the model group rats were given high-fat feed (containing 81.3% basic feed, 3% cholesterol, 0.5% sodium cholate, 0.2% propylthiouracil, 5% white sugar, 10% lard) once a day, and the high-fat feed was fed for 6 weeks after the operation to make the cerebral arteriosclerosis model of hypertension and hyperlipidemia.

[0078] 2. Experimental grouping: The systolic blood pressure (SBP) of the rats was detected, and 176 hypertensive rats (systolic blood pressure SBP≥180mmHg) were selected and randomly divided into a model group and an experimental group, 8 rats in each group. The experimental group was given flaxseed oil or vegetable oil product 0.02mg / g BW by gavage every day, and was continuously gavaged for 6 weeks, and was fed with ordinary feed; the model group was given normal saline 0.02mg / g BW by gavage every day, and was continuously gavaged for 6 weeks, and was fed with ordinary feed.

[0079] The experimental group was divided into Example 1 group-Example 5 group (gavaged with flaxseed oil), Example 6 group-Example 8 group (gavaged with vegetable oil product), Comparative Example 1 group-Comparative Example 8 group (gavaged with flaxseed oil), Comparative Example 9 group-Comparative Example 10 group (gavaged with vegetable oil product), King's Maple Oil group (gavaged with King's Maple Oil), flaxseed oil (commercial) group (gavaged with flaxseed oil (commercial)), sunflower oil group (gavaged with sunflower oil).

[0080] 3. Detection 3.1 TC, TG detection 5mL of blood was taken from the abdominal aorta of the rats, and serum was taken after centrifugation. The TC (total cholesterol) and TG (triglyceride) levels were determined by chemical enzyme method using a full-automatic biochemical analyzer. The detection results are shown in Tables 2.1 and 2.2.

[0081] 3.2 Determination of plasma TXB2 content 3mL of blood was taken from the abdominal aorta of each group of rats, and was mixed in a test tube containing 2% disodium EDTA and aprotinin. The plasma was separated by centrifugation, and the TXB2 (thromboxane B2) was determined by radioimmunoassay. The detection results are shown in Tables 3.1 and 3.2.

[0082] Table 2.1

[0083] In Table 2.1, compared with the model group, & P<0.05, &&P<0.01, indicating that the technical effect has significant difference; compared with Example 1, # P<0.05, ## P<0.01, indicating that the technical effect has significant difference.

[0084] As can be seen from Table 2.1, the linseed oil prepared by the preparation method of the application has a more optimal effect of reducing cholesterol and triglyceride content than ordinary linseed oil on the market and linseed oil prepared by changing the preparation method, and has a significant effect of maintaining the health level of blood lipids (cholesterol / triglyceride).

[0085] Table 2.2

[0086] In Table 2.2, compared with the model group, & P<0.05, && P<0.01, indicating that the technical effect has significant difference; compared with Example 6, # P<0.05, ## P<0.01, indicating that the technical effect has significant difference.

[0087] As can be seen from Table 2.2, the linseed oil prepared by the application is used in the preparation of plant oil products, and is mixed with sunflower oil, safflower oil, walnut oil, pumpkin seed oil, milk thistle seed oil, perilla seed oil, and olive oil, which brings a significant effect of reducing cholesterol and triglyceride content; compared with Comparative Example 9 and Comparative Example 10 which change the ingredients, the effect of maintaining the health level of blood lipids is better; in addition, the effect of reducing TC and TG content of the silver maple oil is better than that of the sunflower oil alone, but in the plant oil product, the linseed oil prepared by the example and the sunflower oil and other ingredients of the plant oil product interact with each other, and cooperate synergistically to significantly reduce the TC and TG content and maintain the health level of blood lipids.

[0088] Table 3.1

[0089] In Table 3.1, compared with the model group, & P<0.05, && P<0.01, indicating that the technical effect has significant difference; compared with Example 1, ## P<0.01, indicating that the technical effect has significant difference.

[0090] Table 3.2

[0091] In Table 3.2, compared with the model group, &P<0.05, && P<0.01, the technical effect has significant difference; compared with example 6, ## P<0.01, the technical effect has significant difference.

[0092] As can be seen from table 3.1 and table 3.2, the TXB2 of the rats in the example group is significantly increased, and there is no significant difference with the normal group; therefore, the flaxseed oil and plant oil product prepared by the present application can protect vascular endothelial cells, balance vascular endothelial dilator and constrictor, and then improve the vasodilation function, so as to achieve the functions of anti-hypertension and maintaining blood pressure to a healthy level.

[0093] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A method for preparing flaxseed oil capable of purifying the cardiovascular system, characterized in that, Including the following steps: (1) Flaxseed is crushed, heated and roasted in stages, and then ground into pulp to obtain flaxseed pulp; (2) Flaxseed pulp was extracted sequentially with water, ethanol and n-hexane to obtain extract mixture A; (3) Extract mixture A and eutectic solvent are mixed and extracted, and the upper layer is taken to obtain extraction mixture B; the eutectic solvent is a mixture of choline chloride and n-butanol; (4) Extract mixture B by removing solvent under reduced pressure, and then perform post-processing steps such as settling, degumming, decolorization and deodorization to obtain flaxseed oil.

2. The preparation method according to claim 1, characterized in that, In step (1), the segmented heating and steaming conditions are set as follows: under moist conditions, steaming and steaming at 60-80℃ for 120-240s, then heating up to 90-110℃ for 30-90s, and finally heating up to 120-130℃ for 20-40s. In step (1), the grinding process specifically involves grinding with a stone mill at 20-30 rpm until no residue remains after twisting.

3. The preparation method according to claim 1, characterized in that, Step (2) specifically involves mixing flaxseed pulp with water at 80-100℃ in several batches, allowing it to stand and separate into layers to obtain an oil layer; the several batches of mixing are 2-4 batches, with the amount of water gradually decreasing in each batch. The oil layer is mixed with ethanol and n-hexane and extracted by stirring at 50-65℃ for 20-40 minutes to obtain extraction mixture A.

4. The preparation method according to claim 1, characterized in that, In step (2), the total amount of water used is 10-20 times the mass of flaxseed; the volume ratio of ethanol, n-hexane and water is 5-15:10-20:50-100; the water refers to all the water used in this step.

5. The preparation method according to claim 1, characterized in that, In step (3), the molar ratio of choline chloride to n-butanol is 1:3-4.

6. The preparation method according to claim 1, characterized in that, In step (3), the volume ratio of the extraction mixture A to the eutectic solvent is 1:3-5.

7. The preparation method according to claim 1, characterized in that, In step (3), the temperature of the mixed extraction is 30-40℃ and the time is 2-4h; In step (4), the settling time is 4-6 hours to remove the precipitate.

8. A flaxseed oil capable of purifying the cardiovascular system, prepared by the preparation method according to any one of claims 1-7.

9. The use of the flaxseed oil of claim 8 in the preparation of a plant oil with the function of purifying the cardiovascular system; the plant oil with the function of purifying the cardiovascular system is not limited to plant oils that can maintain healthy blood pressure levels and maintain healthy blood lipid levels.

10. A plant oil product capable of purifying the cardiovascular system, characterized in that, The product is composed of the following components by weight: 40-50 parts sunflower seed oil, 40-50 parts flaxseed oil as described in claim 8, 0.5-1.5 parts safflower seed oil, 0.5-1.5 parts walnut oil, 0.5-1.5 parts pumpkin seed oil, 0.5-1.5 parts milk thistle seed oil, 0.5-1.5 parts perilla seed oil, and 0.5-1.5 parts olive oil.

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