Urease inhibitors

Abstract

The invention relates to a composition consisting of (a) at least one perfume raw material and (b) the use of EDTA and / or NBPT for inhibiting urease.

Description

TECHNICAL FIELD

[0001] The present invention relates to the field of functional fragrances, in particular to the prevention of the formation of ammonia malodour, more particularly to a mixture endowed with urease inhibiting ability. BACKGROUND

[0002] Fragranced products such as perfumes, deodorants or other fragrance containing products have been widely used in personal care, home care or pet care products to mask unpleasant odours. However, the elimination of ammonia malodour is a major challenge as ammonia not only activates the olfactory system but also triggers trigeminal chemosensation. Trigeminal chemosensation is known to be an alarm system for exposure to chemical hazards (in this case due to pH change) and therefore this sensation is very unpleasant and difficult to mask with traditional fragrances. Therefore, the best way to eliminate ammonia off-notes is to prevent their generation.

[0003] Ammonia can be generated through enzymatic hydrolysis, where urease converts urea in human and animal urine into ammonia and carbamate. Carbamate can spontaneously react to generate another ammonia molecule and carbon dioxide. Urease can originate from bacteria, as is the case in urine, but also from fungi, yeasts and plants. As ammonia is generated over time through enzymatic processes, either the number of urease positive bacteria can be reduced using antibacterial agents or the generation of ammonia can be prevented by chemically inhibiting urease.

[0004] The research on urease inhibition has been quite extensive from a medical point of view as well as from an agricultural product, in particular urea containing fertilizers, point of view. In both fields, potent small molecule inhibitors with high specificity and high efficiency towards urease have been developed. Known inhibitors include, for example, compounds containing urea fragments, flavonoids, quinolones, heterocyclic compounds, organophosphorous compounds and coordination complexes.

[0005] However, many of these compounds cannot be used in personal care, home care and pet care compositions as they are either toxic at high concentrations or have inherent odours that affect the olfactory profile.

[0006] It is therefore an object of the present invention to provide improved urease inhibitors that can be easily used in consumer products to solve the problem of ammonia malodour formation. SUMMARY

[0007] The first object of the present invention relates to a composition consisting of: (a) at least one fragrance raw material and (b) EDTA and / or NBPT for use in inhibiting urease.

[0008] The inventors have surprisingly discovered that the combination of flavoring ingredients with the complexing agents ethylenediaminetetraacetic acid (EDTA) and / or N-(n-butyl)thiophosphate triamine (NBPT) has a synergistic effect on urease inhibition. This is quite surprising because EDTA or NBPT themselves have previously been described as urease inhibitors. The simultaneous use of different urease inhibitors usually presents problems, as they tend to inhibit each other's effectiveness, leading to a decrease rather than an increase in urease inhibition. However, the inventors have found that the combination of flavoring ingredients with EDTA, flavoring ingredients with NBPT, or flavoring ingredients with both EDTA and NBPT not only improves urease inhibition compared to using either substance alone, but also produces a synergistic enhancement. This allows for the creation of compositions that reduce the amount of the CMR substance NBPT and / or the amount of non-biodegradable EDTA without diminishing efficacy.

[0009] Therefore, the present invention can reduce or completely eliminate unpleasant odors and / or other potentially toxic urease inhibitors in compositions, particularly home care, personal care, and pet care compositions. The inventors have also discovered that fragrance ingredients are also capable of removing ammonia generated during chemical reactions. Therefore, the present invention preferably relates to combining initial inhibition of the odor source with interception of the generated odor through chemical reactions, which is an effective technique for reducing or eliminating the perception of ammonia odor. In other words, the present invention also preferably relates to the use of this mixture for inhibiting urease and removing generated ammonia.

[0010] Urease belongs functionally to the amide hydrolase and phosphotriesterase superfamily. Urease is found in various bacteria, fungi, algae, plants, and some invertebrates, and is also present in soil, acting as a soil enzyme. They are high-molecular-weight nickel-containing metalloenzymes. These enzymes catalyze the hydrolysis of urea into carbon dioxide and ammonia.

[0011] The hydrolysis of urea occurs in two stages. The first stage produces ammonia and carbamate. The carbamate spontaneously and rapidly hydrolyzes into ammonia and carbonic acid. Urease activity raises the pH of the surrounding environment because it produces alkaline ammonia.

[0012] The flavoring ingredients, which are part of the mixtures of this invention, are readily available or commercially available, or can be easily prepared. They are highly effective even at commonly used low concentrations, especially at concentrations where the flavoring ingredients are odorless or have at least a very faint odor. They are substantially or completely colorless, highly stable in various mixtures or preparations, and non-toxic and / or non-sensitizing to humans.

[0013] The fragrance ingredients of the present invention also have the following advantages: they can be used in combination with various fragrance ingredients, aromatic oils or essential oils, or commonly used components of aromatic oils, in addition to the fragrance ingredients according to the present invention, thereby adding any desired fragrance to the product. Therefore, the present invention can provide consumers with a variety of fragrance types. In one embodiment of the present invention, only the fragrance ingredients of the present invention, along with EDTA and / or NBPT, are used to flavor the product.

[0014] The "urease inhibitor" according to the present invention is a chemical compound capable of reducing or completely preventing urease activity, and in the present invention, it specifically refers to flavoring raw materials and EDTA and / or NBPT.

[0015] The fragrance ingredients, along with EDTA and NBPT, can be in liquid form, undiluted or diluted with solvents, suitable for use in fragrance blending. Preferred solvents for this purpose include ethanol, isopropanol, diethylene glycol monoethyl ether, glycerol, propylene glycol, 1,2-butanediol, dipropylene glycol, diethyl phthalate, triethyl citrate, isopropyl myristate, glyceryl triacetate, 2,2-dimethyl-1,3-dioxolane-4-methanol, and glyceryl diacetate. A more preferred solvent is triethyl citrate.

[0016] In a preferred embodiment of the invention, the amount of the mixture according to the invention is insufficient to impart its inherent odor when added to a composition or product. An advantage of the mixture according to the invention is that, when used, particularly in applications according to the invention, it already possesses activity for the purposes of the invention at low concentrations where the mixture does not impart, or at least does not impart, a perceptible inherent odor. This allows the mixture to be blended with different types of fragrances (other than those of the invention) and, optionally, other components of aromatic oils, to prepare particularly advantageous fragrance blends, or to additionally add any desired fragrance to aromatic products.

[0017] In a preferred embodiment of the invention, the composition or product is a home care, personal care, or pet care composition or product. Preferred personal care compositions / products are cosmetics and feminine hygiene products, particularly sanitary napkins, diapers, lotions, shampoos, and incontinence pads. Preferred home care compositions / products are toilet care products, such as toilet rim blocks, all-purpose cleaners (APCCleaner), and air fresheners. Preferred pet care compositions / products are pet bedding and pet shampoo.

[0018] The "fragrance raw materials" according to the present invention are raw materials that can be used in fragrance compositions and aromatic oils. Fragrance raw materials can be synthetic or of natural origin. As mentioned above, they have a pleasant aroma and can be readily mixed with other fragrance raw materials according to the present invention or other fragrance raw materials not according to the present invention to produce beneficial fragrance mixtures or aromatic oils.

[0019] In a preferred embodiment of the present invention, the fragrance raw material is selected from fragrance raw materials and essential oils with a molecular weight of 95 to 250 g / mol. Preferably, it is selected from acetals, alcohols, hydrocarbons, aldehydes, ketones, ethers, esters, lactones, and phenols. More preferably, the fragrance raw material with a molecular weight of 95 to 250 g / mol is selected from the group including jasmol, cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 3,7-dimethyloct-2,6-dien-1-ol, cinnamyl alcohol, 4-methyl-2-(2-methylpropyl)tetrahydropyran-4-ol, trans-2-hexenol, cis-3-hexenol, 2-benzylheptanol, geraniol, furanone, citral, 3-(4-tert-butylphenyl)butanol, 3-(4-ethylhexyl)butanol, etc. 2,2-Dimethylpropanal (2-ethylphenyl)-2,2-dimethylpropanal, C12 aldehyde, trans-2-hexenal, (±)-2,6-dimethyl-5-heptenal, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxal, cyclamate aldehyde, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxal (68039-49-6; 68737-61-1; Ligustral, Tripalal, CyclalC, Vertocit ral), α-hexylcinnamaldehyde, (2E)-3,7-dimethyloctyl-2,6-dienal, 3-(4-ethylphenyl)-2,2-dimethylpropional, helional, cis-3-hexenyl hexanoate, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvate, trans-carvate, 1,6-dihydro-carvate, 1,6-dihydro-neo-carvate, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-salicylic acid Hexenyl ester, NerolinYaraYara, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (698-10-2), γ-terpinene, ocimene (Ocimene), β-ionone, N-methylionone, methylheptanone, α-damasone, α-methylionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol.

[0020] In another preferred embodiment of the invention, the fragrance with a molecular weight of 95 to 250 g / mol is selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 3,7-dimethyloctyl-2,6-dien-1-ol, cinnamyl alcohol, 4-methyl-2-(2-methylpropyl)tetrahydropyran-4-ol, trans-2-hexenol, cis-3-hexenol, 2-benzylheptanol, geraniol, furanone, citral, 3-(4-tert-butylphenyl)butanol, 3-(4-ethylhexyl)butanol, etc. 2,2-Dimethylpropanal (2-ethylphenyl)-2,2-dimethylpropanal, C12 aldehyde, (±)-2,6-dimethyl-5-heptenal, (Z,E)-2,4-dimethylcyclohexyl-3-en-1-carboxal, cyclamate aldehyde, (Z,E)-2,4-dimethylcyclohexyl-3-en-1-carboxal (68039-49-6; 68737-61-1; Ligustral, Tripalal, CyclalC, Vertocitral), α-Hexylcinnamaldehyde, (2E)-3,7-dimethyloctyl-2,6-dienal, 3-(4-ethylphenyl)-2,2-dimethylpropanal, Helional, cis-3-hexenyl hexanoate, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvate, trans-carvate, 1,6-dihydro-carvate, 1,6-dihydro-neo-carvate, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate NerolinYaraYara, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (698-10-2), γ-terpinene, ocimene (Ocimene), β-ionone, N-methylionone, methylheptanone, α-damasconone, α-methylionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasconone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol.

[0021] In a more preferred embodiment of the invention, the fragrance having a molecular weight of 95 to 250 g / mol is selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), cis-3-hexenol, 2-benzylheptanol, geraniol, furanone, cyclamate, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxaldehyde (68039-49-6;68737-61-1; ligustral, Tripalal, Cyclal C, Vertocitral), α-hexylcinnamaldehyde, (2E)-3,7-dimethyloctyl-2,6-dienal, 3-(4-ethylphenyl)-2,2-dimethylpropanal, and helizardaldehyde. (onal), cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvate, trans-carvate, 1,6-dihydro-carvate, 1,6-dihydro-neo-carvate, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (698-10-2), γ-terpinene, ocimene, methyl heptanone, α-damasone, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol.

[0022] In one or even a more preferred embodiment of the invention, the fragrance having a molecular weight of 95 to 250 g / mol is selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, and cis-... -Carvyl acetate, trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol.

[0023] Preferably, the essential oil according to the present invention comprises at least one of the following compounds as a main component: eucalyptol, geraniol, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). 、(1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol(5986-55-0, Patchouliaclohol)), p-menth-1,8-diene(5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene(498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene(1461-03-6, β-cedrene).

[0024] In another aspect of the invention, at least one flavoring ingredient is selected from the group consisting of:

[0025] As described in the experimental section, the flavoring raw materials exhibit varying degrees of urease inhibition. Although many flavoring raw materials do not show inhibitory effects, the inventors have discovered that some flavoring raw materials have inhibition levels of: Level 1 (inhibition rate ≥ 0.3 and < 0.5), Level 2 (inhibition rate ≥ 0.5 and < 0.75), Level 3 (inhibition rate ≥ 0.75 and < 0.9), or even Level 4 (inhibition rate ≥ 0.9). Preferably, based on the tests conducted in the examples, flavoring raw materials with inhibition levels of Level 2, Level 3, or Level 4 are selected; more preferably, Level 3 or Level 4; and most preferably, flavoring raw materials with Level 4 are selected.

[0026] Therefore, the preferred spice ingredients are selected from the following group:

[0027] In another preferred embodiment, the spice ingredients are selected from the group consisting of:

[0028] In another preferred embodiment, the spice ingredients are selected from the group consisting of:

[0029] In another preferred embodiment, the spice ingredients are selected from the group consisting of:

[0030] In another preferred embodiment, the spice ingredients are selected from the group consisting of:

[0031] In each different implementation, all the mentioned flavoring ingredients may be used in combination with EDTA or NBPT or NBPT and EDTA.

[0032] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one fragrance ingredient having a molecular weight of 95 to 250 g / mol, selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvyl acetate, Trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damaslenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol; and (b)EDTA Used to inhibit urease.

[0033] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one fragrance ingredient having a molecular weight of 95 to 250 g / mol, selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvyl acetate, Trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damaslenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol; and (b)NBPT Used to inhibit urease.

[0034] In one aspect of the invention, the amount of at least one flavoring ingredient in the mixture is 0.0005 to 99.9995% by weight, preferably 0.005 to 99.995% by weight, more preferably 25 to 99.99% by weight, and most preferably 50 to 99.9% by weight, based on the total amount of the mixture.

[0035] In one aspect of the invention, the amount of EDTA used is 0.01 to 75% by weight, preferably 0.01 to 50% by weight, more preferably 1.0 to 35% by weight, based on the total amount of the mixture.

[0036] In one aspect of the invention, the amount of NBPT is 0.0000001 to 25% by weight, preferably 0.00001 to 20% by weight, more preferably 0.0001 to 10% by weight, and most preferably 0.001 to 2% by weight, based on the total amount of the mixture.

[0037] In one aspect of the invention, the weight ratio of the at least one flavoring ingredient to EDTA is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:1.

[0038] In one aspect of the invention, the weight ratio of the at least one flavoring ingredient to NBPT is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1, and most preferably 200:1 to 30:1.

[0039] It is advantageous to prepare the components of the mixture. It is preferred to microencapsulate, spray-dry, or prepare as inclusion complexes or extruders, preferably with flavoring ingredients and / or EDTA, more preferably with EDTA only.

[0040] In one aspect, the invention is applied to a personal care composition comprising a mixture of the following components: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0041] In one aspect of the invention, the invention is applied to a personal care composition, wherein... (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:1.

[0042] In another aspect of the invention, the invention is applied to the personal care composition according to claim 20, wherein... (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1 and most preferably 200:1 to 30:1.

[0043] In another aspect, the invention is applied to a home care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0044] In another aspect, the invention is applied to a home care composition, wherein (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1 and most preferably 200:1 to 30:1.

[0045] In another aspect, the invention is applied to a home care composition, wherein (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:1. Preferably, the household care composition is a toilet rim block or an APC (Advanced Particulate Scrubber). The effects of this invention are so significant that the household care composition can be used with water with a hardness greater than 7°, preferably greater than 10°, and more preferably greater than 14°. This is surprising because water hardness typically has a negative impact on the performance of EDTA.

[0046] In another aspect, the invention is applied to a pet care composition comprising a mixture of the following components: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0047] In another aspect, the invention is applied to a pet care composition, wherein... (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1 and most preferably 200:1 to 30:1.

[0048] In another aspect, the invention is applied to a pet care composition, wherein... (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:1.

[0049] In a preferred embodiment of the invention, the personal care, home care, or pet care composition does not contain any other urease inhibitors.

[0050] In another preferred embodiment, the personal care, home care, or pet care composition according to the invention comprises a fragrance ingredient selected from fragrance ingredients and essential oils with a molecular weight of 95 to 250 g / mol.

[0051] In another preferred embodiment, the personal care, home care, or pet care composition according to the invention comprises a fragrance ingredient selected from molecules with a test result of 4.

[0052] In another preferred embodiment, the personal care, home care, or pet care composition according to the invention comprises a fragrance ingredient selected from molecules with a test result of 3.

[0053] Another aspect of the invention relates to a method for inhibiting urease activity in a personal care, home care, or pet care composition, wherein the mixture comprises the following components: (a) at least one spice ingredient and (b) EDTA and / or NBPT Add to personal care, home care, or pet care compositions.

[0054] In a preferred method for inhibiting urease activity in a personal care, home care, or pet care composition, the mixture is added to the personal care, home care, or pet care composition at a weight percentage of 0.01 to 10%, based on the total weight of the personal care, home care, or pet care composition.

[0055] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one essential oil, wherein the essential oil comprises at least one of the following compounds as a major component: eucalyptol, geranialdehyde, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). ), (1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol (5986-55-0, Patchouliaclohol), p-menth-1,8-diene (5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene (498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene (1461-03-6, β-cedrene); and (b)EDTA Used to inhibit urease.

[0056] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one essential oil, wherein the essential oil comprises at least one of the following compounds as a major component: eucalyptol, geranialdehyde, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). ), (1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol (5986-55-0, Patchouliaclohol), p-menth-1,8-diene (5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene (498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene (1461-03-6, β-cedrene); and (b)NBPT Used to inhibit urease.

[0057] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one fragrance ingredient having a molecular weight of 95 to 250 g / mol, selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvyl acetate, Trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damaslenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol; and At least one essential oil, wherein the essential oil comprises at least one of the following compounds as a main component: eucalyptol, geranialdehyde, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). 、(1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol (5986-55-0, Patchouliaclohol), p-menth-1,8-diene (5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene (498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene (1461-03-6, β-cedrene); and (b)EDTA.

[0058] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one fragrance ingredient having a molecular weight of 95 to 250 g / mol, selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvyl acetate, Trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damaslenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol; and At least one essential oil, wherein the essential oil comprises at least one of the following compounds as a main component: eucalyptol, geranialdehyde, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). 、(1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol (5986-55-0, Patchouliaclohol), p-menth-1,8-diene (5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene (498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene (1461-03-6, β-cedrene); and (b)NBPT.

[0059] Preferably, the present invention relates to a mixture comprising the following components: (a) At least one fragrance ingredient having a molecular weight of 95 to 250 g / mol, selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 2-benzylheptanol, geraniol, furanone, 3-(4-ethylphenyl)-2,2-dimethylpropionaldehyde, helional, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvyl acetate, Trans-carvyl acetate, 1,6-dihydro-carvyl acetate, 1,6-dihydro-neo-carvyl alcohol, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, ocimene, α-methyl ionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damaslenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol; and At least one essential oil, wherein the essential oil comprises at least one of the following compounds as a main component: eucalyptol, geranialdehyde, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azulene-6-ol (68129-81-7, Vetiverol). 、(1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol (5986-55-0, Patchouliaclohol), p-menth-1,8-diene (5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene (498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene (1461-03-6, β-cedrene); and (b) EDTA and NBPT.

[0060] In a preferred embodiment of the invention, at least one flavoring ingredient is used in an amount of 0.1 to 75% by weight, based on the total amount of the mixture. Preferably, EDTA is used in an amount of 0.1 to 50% by weight, based on the total amount of the mixture. Preferably, NBPT is used in an amount of 0.01 to 25% by weight, based on the total amount of the mixture.

[0061] In a preferred embodiment of the present invention, the ratio of at least one flavoring ingredient to EDTA and / or NBPT is 1:5 to 5:1.

[0062] It goes without saying that one or more flavoring ingredients can be used to inhibit urease activity. Therefore, in the mixtures according to the invention, one, two, three, four, five, six, seven, eight, nine, ten, or even twenty to thirty flavoring ingredients according to the invention can be used to inhibit urease activity.

[0063] Another embodiment of the present invention relates to a personal care composition comprising a mixture of the following components: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0064] Another embodiment of the present invention relates to a home care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0065] Another embodiment of the present invention relates to a pet care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

[0066] Preferably, when the mixture is added to the composition or product, its dosage is 0.01 to 10% by weight, based on the total weight of the composition or product. More preferably, the dosage of the mixture is 0.05 to 5% by weight; even more preferably, its dosage is 0.05 to 1% by weight; most preferably, its dosage is 0.1 to 1% by weight; all weight percentages are based on the total weight of the composition or product. When the mixture according to the invention is used in a toilet rim block, preferably, its dosage is 0.01 to 10% by weight; more preferably, its dosage is 1 to 8% by weight; even more preferably, its dosage is 2 to 6% by weight; most preferably, its dosage is 2.5 to 4.5% by weight, all dosages are based on the total weight of the composition or product.

[0067] Particularly advantageous is one use according to the invention, wherein the ratio of the total mass of the mixture to the total mass of at least one ammonia precursor is 2:1 to 1:5, more preferably 1:1 to 1:3.

[0068] According to the present invention, a "precursor" refers to a compound that participates in a chemical reaction to generate another compound. In this example, an "ammonia precursor" refers to a compound that participates in a chemical reaction to generate ammonia. In one embodiment of the present invention, the ammonia precursor is urea.

[0069] Therefore, in a preferred embodiment of the invention, the mixture can be used as a urease inhibitor in compositions or products, particularly in home care, personal care, and pet care compositions / products, and in conjunction with at least one ammonia precursor. Thus, when in contact with, for example, bacterial urease, the formation of ammonia can be completely, but at least partially, prevented by the mixture of the invention.

[0070] It goes without saying that one or more fragrance ingredients can be used in the mixture to inhibit urease activity. Therefore, for example, in home care, personal care, or pet care compositions / products, 1, 2, 3, 4, 5, 6, 7, 8, 9, or even 50 fragrance ingredients with a molecular weight between 95 and 250 g / mol can be used to inhibit urease activity.

[0071] Examples of other flavorings that can be advantageously combined with other ingredients in a mixture can be found, for example, in S. Arctander, Perfume and Flavor Chemicals, Volumes I and II, Montclair, NJ, 1969, self-published, or H. Surburg, J. Panten, Common Fragrance and Flavor Materials, Fifth Edition, Wiley-VCH, Weinheim 2006. In some embodiments of the invention, the mixtures of the invention may be mixed with other fragrances or solvents. The mixtures of the invention may be liquid, undiluted or diluted with a solvent, and are preferably used for fragrance blending. Preferred solvents for this purpose are ethanol, isopropanol, diethylene glycol monoethyl ether, glycerol, propylene glycol, 1,2-butanediol, dipropylene glycol, diethyl phthalate, triethyl citrate, isopropyl myristate, glyceryl triacetate, 2,2-dimethyl-1,3-dioxolane-4-methanol, and glyceryl diacetate.

[0072] Furthermore, the mixture according to the invention can be adsorbed onto a carrier that enables both uniform dispersion of the fragrance raw materials in the product and controlled release upon application. Such carriers can be porous inorganic materials, such as light sulfates, silica gel, zeolite, gypsum, clay, clay particles, aerated concrete, etc.; or organic materials, such as wood, cellulose-based materials, sugars, dextrin (e.g., maltodextrin); or plastics, such as polyvinyl chloride (PVC), polyvinyl acetate, or polyurethane.

[0073] The mixture according to the invention can also be microencapsulated, spray-dried, used as an inclusion compound or extruded, and can be added in this form to, for example, a product to be flavored. Preferably, the mixture is processed in accordance with the manner described in US2009253612A1 or US7235261B2.

[0074] If necessary, the properties of the composition can be further optimized by so-called “coating” with suitable materials to release fragrance more effectively; waxy plastics, such as polyvinyl alcohol, are preferred for this purpose.

[0075] Preferably, the personal care, home care, or pet care composition according to the invention does not contain any other urease inhibitors.

[0076] Of course, other preferred embodiments and definitions described above also apply to personal care, home care and pet care compositions / products according to the present invention.

[0077] Another embodiment of the invention relates to a method for inhibiting urease activity in a personal care, home care, or pet care composition, wherein a composition is comprising the following components: (a) at least one spice ingredient; (b) EDTA and / or NBPT Add to personal care, home care, or pet care compositions.

[0078] Preferably, the mixture is added in an amount of 0.01 to 10% by weight, based on the total weight of the personal care, home care, or pet care composition. More preferably, the mixture is added in an amount of 0.05 to 5% by weight, based on the total weight of the personal care, home care, or pet care composition. Even more preferably, the mixture is added in an amount of 0.05 to 1% by weight, based on the total weight of the personal care, home care, or pet care composition. When the mixture according to the invention is added to a toilet rim block, its preferred amount is 0.01 to 10% by weight, more preferably 1 to 8% by weight, more preferably 2 to 6% by weight, and even more preferably 2.5 to 4.5% by weight, based on the total weight of the composition or product.

[0079] The preferred embodiments and definitions described above also apply to the methods according to the present invention.

[0080] The embodiments of the present invention will be further described below.

[0081] Unless otherwise stated, all amounts are expressed as a percentage by weight. Detailed Implementation

[0082] Example 1 Screening for urease inhibition Material: Stock solution: PBS2x: PBS2× solution is prepared by diluting PBS10×. Add approximately 0.5 mg of phenol red to this diluted solution. Adjust the pH to 5–6 using 37% hydrochloric acid (HCl). The optimal color of the solution is yellow.

[0083] ●Urea: Prepare a 10g / L urea stock solution in distilled water.

[0084] ●MasterMix—PBS and urea stock solution: 12mL 2×PBS + 2.4mL urea stock solution + 6mL distilled water.

[0085] ●Urease: Urease (Sigma Aldrich, 500–800 U / mL) derived from sword bean (Canavalia ensiformis, Jackbean) was used to prepare a urease solution in distilled water with an activity of approximately 20 U / mL.

[0086] ●FRM / NBPT (as PS): For liquid samples, use a micropipette to transfer 80 µL of the sample to a 5 mL bottle. Dilute with 4 mL DMSO. This solvent is chosen because it does not inhibit urease activity or ammonia formation. For solid samples, weigh 80 µg of the sample using a precision balance and transfer it to a 5 mL bottle. Dilute with 4 mL DMSO.

[0087] Test method: The test was performed using a 96-well plate in an optical microplate reader. Without the addition of inhibitors, urea reacts with urease to produce ammonia, causing a change in the pH of the solution and a color change from yellow to pink. This color change, as an indirect indicator of ammonia production, was detected and measured using an optical lens, generating a graph of optical density (560 nm) over time.

[0088] preparation: 1. Add 10µl of fragrance raw material (FRM), NBPT (PS) and DMSO (NS) to the well.

[0089] 2. Then, add 20 µl of urease (for blank samples, use distilled water instead).

[0090] 3. Add 170 µl of MasterMix containing urea substrate to the mixture in the well and shake well.

[0091] The reaction may start rapidly, so the reaction plate should be placed in the microplate reader before the color changes.

[0092] Experimental procedure: The test was conducted for 1 hour at a set temperature of 24°C. Readings were taken at a wavelength of 560 nm using a kinetic method. Measurement intervals were 1 minute.

[0093] Table 1: Final concentrations detected:

[0094] Inhibition value: The reaction rate is determined by color (at 560 nm). The change in absorbance over time was obtained as follows:

[0095] Inhibitory efficacy is calculated as follows:

[0096] Identification of positive results Figure 1 Typical results from the screening test are shown. The corresponding calculation results are shown in Table 2. The results indicate that this flavoring raw material has a significant inhibitory effect on urease.

[0097] Table 2: Screening Test Results

[0098] Positive result A total of 56 highly reactive compounds were identified through in vitro screening. Interestingly, they share common structural characteristics, such as molecular weights ranging from 95 to 250 g / mol, and are all acetals, alcohols, hydrocarbons, aldehydes, ketones, ethers, esters, lactones, and phenols.

[0099] Table 3: Highly Active Compounds

[0100] Another ingredient list also showed very good performance.

[0101]

[0102] Raw material screening The concentration of the flavoring raw material is 0.1%, based on a 4% FRA dosage. The reduction in ammonia production is based on urease inhibition; if urease is not completely inhibited, the generated ammonia is removed through subsequent chemical reactions. Figure 2 and Figure 3 The results are displayed.

[0103] Fragrance (accord) selection Fragrance concentration: The total concentration of the fragrance composition in the toilet rim block is 4%. The fragrance accounts for 1.2% of the total FRA content, meaning that this fragrance makes up 30% of the total FRA. The remainder is filled with DPG. Figure 4 Figures 5 and 6 show the results.

[0104] Fragrance composition Fragrance type AB

[0105] Fragrance AC

[0106] Fragrance AD

[0107] Example 2 Toilet rim block Add 47 mL of urea solution (50 g / L, pH 5.0) to a 250 mL Duran flask. Then add 500 mg of edge block substrate containing FRA, EDTA, or NBPT. Initiate the reaction by adding 500 µL of urease solution (500-800 U / mL) while stirring (80 rpm). The edge block substrate should not come into contact with the stir bar. Figure 2 A typical setup is shown. Ammonia concentration was determined using a sensitive ammonia analyzer. Measurement time points were selected at 15 minutes, 2 hours, 4 hours, 6 hours, 24 hours, 26 hours, and 30 hours. Measurement was stopped once steady state was reached. Figure 1 This demonstrates a typical reaction process.

[0108] The negative control group had DPG as filler in the toilet rim block (the efficiency of DPG had been pre-tested – no inhibitory effect was observed in the analytical-scale test and in the rim block matrix).

[0109] The positive control group had 0.1% Neofresh ammonia (containing 0.025% NBPT) and 3.9% DPG added to the toilet rim block.

[0110] The test samples contained 0.1% to 1% EDTA or 0.1% to 4% FRM. Whenever the material content fell below 4%, the remaining portion was filled with DPG.

[0111] The aroma profile, composed of the FRM that performed best in the enzymatic assay, was incorporated into the edge block matrix at different concentrations ranging from 0.1% to 4% to verify its concentration-dependent inhibitory properties.

[0112] Example 3: Cooperative Behavior

[0113] Adding FRA-flavored AC can achieve an inhibition efficiency comparable to 1% EDTA.

[0114] Due to the synergistic effect of flavor and EDTA, the amount of non-biodegradable EDTA used can be reduced.

[0115] Example 4: Cooperative Behavior

[0116] The combination of spray-dried EDTA and FRA fragrance resulted in 3.3 times higher activity than FRA fragrance alone.

[0117] The combination of a lower concentration of EDTA (0.2% in spray-dried formulations) with FRA-flavored AD had an inhibitory effect comparable to that of 1% EDTA (PosC).

[0118] Example 5: Cooperative Behavior

[0119] Synergistic effects can be observed when fragrances are used in combination with the non-FRA substance NBPT.

[0120] Similar inhibitory effects were observed when used in combination with higher concentrations of NBPT alone.

[0121] This allows for a reduction in the proportion of the CMR substance NBPT, and the efficiency reduction can be compensated for by the synergistic effect of adding FRA fragrance, thereby significantly reducing the toxicity of the system.

Claims

1. A composition comprising the following components: (a) at least one spice ingredient and (b) EDTA and / or NBPT Used to inhibit urease.

2. The use according to claim 1, wherein the fragrance raw material is selected from fragrance raw materials and essential oils with a molecular weight of 95 to 250 g / mol.

3. The use according to claim 1 and / or 2, wherein the fragrance raw material with a molecular weight of 95 to 250 g / mol is selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 3,7-dimethyloctyl-2,6-dien-1-ol, cinnamyl alcohol, 4-methyl-2-(2-methylpropyl)tetrahydropyran-4-ol, trans-2-hexenol, cis-3-hexenol, 2-Benzylheptanol, Geraniol, Furanone, Citral, 3-(4-tert-butylphenyl)butanal, 3-(4-ethylphenyl)-2,2-dimethylpropanal, 3-(2-ethylphenyl)-2,2-dimethylpropanal, C12 aldehyde, trans-2-hexenal, (±)-2,6-dimethyl-5-heptenal, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxaldehyde, Cyclamenal, (Z,E)-2,4-dimethyl... Cyclohexyl-3-en-1-carboxaldehyde (68039-49-6; 68737-61-1; Ligustral, Tripalal, CyclalC, Vertocitral), α-hexylcinnamaldehyde, (2E)-3,7-dimethyloctyl-2,6-dienal, 3-(4-ethylphenyl)-2,2-dimethylpropanal, Helional, etc. Cis-3-hexenyl acetate, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvate, trans-carvate, 1,6-dihydrocarvate, 1,6-dihydro-neo-carvate, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate, cis-3-hexenyl salicylate, NerolinYaraYara, 5-ethyl-3-hydroxy-4-methyl-2(5 H )-furanone (698-10-2), γ-terpinene, ocimene, β-ionone, N-methylionone, methylheptanone, α-damasone, α-methylionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasenone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone and eugenol.

4. The use according to claim 1 and / or 2, wherein the fragrance raw material having a molecular weight of 95 to 250 g / mol is selected from the group comprising cis-1-(1-ethoxyethoxy)-3-hexene (leafacetal), 3,7-dimethyloctyl-2,6-dien-1-ol, cinnamyl alcohol, 4-methyl-2-(2-methylpropyl)tetrahydropyran-4-ol, trans-2-hexenol, cis-3-hexenol, 2-benzylheptanol, geraniol, furanone, citral, 3-(4-tert-butylphenyl)butanol, 3 -(4-Ethylphenyl)-2,2-dimethylpropanal, 3-(2-Ethylphenyl)-2,2-dimethylpropanal, C12 aldehyde, trans-2-hexenal, (±)-2,6-dimethyl-5-heptenal, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxal, cyclamate aldehyde, (Z,E)-2,4-dimethylcyclohex-3-en-1-carboxal (68039-49-6;68737-61-1; Ligustral, Tripalal, Cyclal C,V ertocitral), α-hexylcinnamaldehyde, (2E)-3,7-dimethyloctyl-2,6-dienal, 3-(4-ethylphenyl)-2,2-dimethylpropional, helional, cis-3-hexenyl hexanoate, cis-3-hexenyl butyrate, cis-3-hexenyl acetate, cis-3-hexenyl isovalerate, cis-carvate, trans-carvate, 1,6-dihydro-carvate, 1,6-dihydro-neo-carvate, cis-3-hexenyl propionate, cis-3-hexenyl isobutyrate Salicylic acid cis-3-hexenyl ester, NerolinYaraYara, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (698-10-2), γ-terpinene, ocimene, β-ionone, N-methylionone, methylheptanone, α-damasconone, α-methylionone, methylcyclopentenolone, 2-methyl-5-propyl-2-cyclohexenone, β-damasconone, 1-(5,5-dimethyl-1-cyclohexenyl)pent-4-en-1-one, dimethylbenzofuranone, and eugenol.

5. The use according to claim 1 and / or 2, wherein the essential oil comprises at least one of the following compounds as a main component: eucalyptol, geraniol, citronellol, linalool, α-pinene (80-65-8), β-pinene (127-91-3), eugenol, (1E,6E,8S)-1-methyl-5-methylene-8-prop-2-ylcyclodec-1,6-diene (23986-74-5, GermacrenD), 4,8-dimethyl-2-prop-2-ylidene-3,3a,4,5,6,8a-hexahydro-1H-azine-6-ol (68129-81-7, Vetiverol). rol), (1R,3R,6S)-2,2,6,8-tetramethyl-tricyclo[5.3.1.03,8]undec-3-ol(5986-55-0, Patchouliaclohol), p-menth-1,8-diene(5989-27-5, limonene D), (+)-3-carene, (1S)-3,7,7-trimethyl-bicyclo[4.1.0]hept-3-ene(498-15-7, δ-3-carene), 2,5,9,9-tetramethyl-3,4,6,7,8,9a-hexahydrobenzo[7]cycloene(1461-03-6, β-cedrene).

6. The use according to claim 1 and / or 2, wherein the fragrance ingredient comprises at least one fragrance ingredient selected from the group consisting of: 。 7. The use according to at least one of the preceding claims, wherein the test result of the experimental inhibition rate is Level 1 (inhibition rate ≥ 0.3 and < 0.5), Level 2 (inhibition rate ≥ 0.5 and < 0.75), Level 3 (inhibition rate ≥ 0.75 and < 0.9), or Level 4 (inhibition rate ≥ 0.9), preferably Level 2, Level 3, or Level 4, more preferably Level 3 or Level 4, and most preferably Level 4.

8. The use according to claim 1 and / or 2, wherein the fragrance raw material is selected from the group consisting of: 。 9. The use according to claim 1 and / or 2, wherein the fragrance raw material is selected from the group consisting of: 。 10. The use according to claim 1 and / or 2, wherein the fragrance ingredient is selected from the group consisting of: 。 11. The use according to claim 1 and / or 2, wherein the fragrance raw material is selected from the group consisting of: 。 12. The use according to claim 1 and / or 2, wherein the fragrance raw material is selected from the group consisting of: 。 13. The use according to at least one of the preceding claims, wherein the amount of at least one flavoring ingredient is 0.0005 to 99.9995% by weight, preferably 0.005 to 99.995% by weight, more preferably 25 to 99.99% by weight, and most preferably 50 to 99.9% by weight, based on the total amount of the mixture.

14. The use according to at least one of the preceding claims, wherein the amount of EDTA is 0.01 to 75% by weight, preferably 0.01 to 50% by weight, more preferably 1.0 to 35% by weight, based on the total amount of the mixture.

15. The use according to at least one of the preceding claims, wherein the amount of NBPT is from 0.0000001 to 25% by weight, preferably from 0.00001 to 20% by weight, more preferably from 0.0001 to 10% by weight, and most preferably from 0.001 to 2% by weight, based on the total amount of the mixture.

16. The use according to at least one of the preceding claims, wherein the weight ratio of at least one flavoring ingredient to EDTA is 1:20 to 100:1, preferably 1:10 to 50:1, more preferably 1:5 to 25:

1.

17. The use according to at least one of the preceding claims, wherein the weight ratio of at least one flavoring ingredient to NBPT is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1, and most preferably 200:1 to 30:

1.

18. The use according to at least one of the preceding claims, wherein one or more components are microencapsulated, spray-dried, used as inclusion complexes or as extruders, preferably flavoring ingredients and / or EDTA, more preferably EDTA only.

19. The use according to at least one of the preceding claims, wherein the mixture is in a liquid state, preferably undiluted, more preferably diluted with a solvent, and even more preferably diluted with a solvent selected from the group consisting of: ethanol, isopropanol, diethylene glycol monoethyl ether, glycerol, propylene glycol, 1,2-butanediol, dipropylene glycol, diethyl phthalate, triethyl citrate, isopropyl myristate, triacetin, 2,2-dimethyl-1,3-dioxolane-4-methanol, and diacetin, with triethyl citrate being the most preferred diluent.

20. A personal care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

21. The personal care composition of claim 20, wherein... (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:

1.

22. The personal care composition of claim 20, wherein... (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1 and most preferably 200:1 to 30:

1.

23. A home care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

24. The home care composition according to claim 23, wherein... (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, and most preferably 200:1 to 1:

1.

25. The home care composition according to claim 23, wherein... (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:

1.

26. A pet care composition comprising a mixture of the following ingredients: (a) at least one spice ingredient and (b) EDTA and / or NBPT.

27. The pet care composition of claim 26, wherein... (a) at least one spice ingredient and (b)NBPT The weight ratio is 10000:1 to 1:5, preferably 1000:1 to 1:2, more preferably 200:1 to 1:1 and most preferably 200:1 to 30:

1.

28. The pet care composition of claim 26, wherein (a) at least one spice ingredient and (b)EDTA The weight ratio is 1:20 to 100:1, preferably 1:10 to 50:1, and more preferably 1:5 to 25:

1.

29. The personal care, home care, or pet care composition according to any one of claims 20 to 28, wherein the composition does not contain any other urease inhibitors.

30. The personal care, home care, or pet care composition according to any one of claims 20 to 28, wherein the fragrance ingredient is selected from fragrance ingredients and essential oils with a molecular weight of 95 to 250 g / mol.

31. The personal care, home care, or pet care composition according to any one of claims 20 to 28, wherein the fragrance ingredient is selected from molecules with a test result of 4.

32. The personal care, home care, or pet care composition according to any one of claims 20 to 28, wherein the fragrance ingredient is selected from molecules with a test result of 3.

33. A method for inhibiting urease activity in a personal care, home care, or pet care composition, wherein the mixture comprises the following components (a) at least one spice ingredient and (b) EDTA and / or NBPT Add to personal care, home care, or pet care compositions.

34. The method of claim 29, wherein 0.01 to 10% by weight of the mixture is added to the personal care, home care, or pet care composition, based on the total weight of the personal care, home care, or pet care composition.

35. The home care composition according to claim 23 and / or 25, wherein the home care composition is a toilet rim block or all-purpose cleaner, and can be used in water with a hardness greater than 7°, preferably greater than 10°, more preferably greater than 14°.

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