Long-distance water-carrying transportation system and method for egg-carrying blue crabs based on dynamic regulation and control of microenvironment water body

Abstract

The invention belongs to the technical field of aquaculture living body transportation, and discloses an egg-carrying blue crab long-distance water-carrying transportation system and method based on microenvironment water body dynamic regulation and control. Mechanical stimulation of vibration to parents and embryos in the transportation process is reduced or even eliminated; active constant temperature is achieved through the phase-change material temperature control layer, and it is ensured that the water microenvironment is stable in the whole transportation process; beneficial microorganisms and adsorbents are loaded on the bag wall of the hydrophilic liner to realize in-situ purification of water, so that a'poison-oxygen deficiency 'vicious circle formed by metabolic waste accumulation and dissolved oxygen reduction is avoided; the cheliped is fixed by adopting the water-soluble ribbon, so that secondary stress caused by removing constraint after the cheliped is transported is reduced; by establishing a transport time window model based on an embryonic development time sequence, accurate planning of transport behaviors is realized.

Description

Technical Field

[0001] This invention belongs to the field of live animal transportation technology in aquaculture, and particularly relates to a long-distance water-carrying system and method for berried mud crabs based on dynamic regulation of the microenvironment water body. Background Technology

[0002] The mud crab (Scylla serrata) is an important aquaculture species in the southeastern coastal region of my country. The sustainable development of its artificial breeding and aquaculture industry relies on a stable and high-quality source of parent crabs. As the direct parent for seed production, the quality and quantity of berried crabs directly determine the success and scale of seed production. With the expansion of mud crab aquaculture and the increase in seed farms, the demand for cross-regional allocation of high-quality berried crabs is increasing. Achieving safe and efficient long-distance transportation has become a key link in ensuring the optimal allocation of mud crab seed resources and the healthy development of the industry. Unlike ordinary commercial crabs or non-berried parent crabs, berried crabs face more severe challenges during transportation. Their unique characteristic lies in the fact that the embryos they carry are living organisms undergoing continuous metabolism and are extremely sensitive to changes in external environmental conditions. Stress factors such as mechanical vibration, water temperature fluctuations, and insufficient dissolved oxygen during transportation can not only damage the health of the parent crabs but may also lead to embryonic development arrest, detachment, or even death. In existing technologies, Li Lirong et al. successfully transported berried Chinese mitten crabs to a hatchery 350 kilometers away using a method of layering aquatic plants with berried crabs, achieving a 100% survival rate during transport. Furthermore, the transported berried crabs successfully hatched into larvae. Gao Baoquan et al. successfully transported berried swimming crabs from the north to a hatchery in the south by wrapping their egg masses in damp towels under low temperature and aeration conditions, achieving similarly ideal results. However, due to the lack of systematic buffer design and dynamic water quality management in these methods, temperature control is relatively passive. When transporting aggressive mud crabs with large egg loads, problems such as low embryo hatching rates and high parent mortality rates often occur. Summary of the Invention

[0003] To address the problems existing in the prior art, this invention provides a long-distance water-carrying transport system and method for berried mud crabs based on dynamic regulation of the microenvironment water body.

[0004] This invention is implemented as follows: a long-distance water-borne transport system for berried mud crabs based on dynamic regulation of the microenvironment water body, comprising:

[0005] Multifunctional composite inner bag, intelligent outer box system, supporting components, emergency handling kit;

[0006] The multifunctional composite inner liner bag includes a cylindrical oxygen filling and binding opening and a composite inner liner bag made of three layers of composite fabric.

[0007] The composite inner liner bag is made of three layers of composite fabric. The outer layer is high-strength waterproof canvas. The middle layer of the composite inner liner bag is made of three layers of composite fabric. The inner layer is made of three layers of composite fabric. The inner layer is made of microfiber hydrophilic fleece.

[0008] The intelligent outer casing system includes paraffin-based composite materials, silicone, springs, a miniature water quality detector, and a control and display panel, all filled inside a foam box.

[0009] Silicone and springs constitute a spring-silicone composite shock absorption module;

[0010] The miniature water quality detector is connected to the control and display panel via electrical wiring.

[0011] Furthermore, the multifunctional composite inner liner bag is made of three layers of composite fabric; the inner layer is a microfiber hydrophilic fleece, simulating the texture of natural seaweed attachment substrate to reduce embryo friction and mechanical damage; the middle layer is a high-elasticity silicone damping layer to absorb high-frequency micro-vibrations; the outer layer is a high-strength waterproof canvas; the bag body is a cuboid, 50 cm long, 30 cm wide, and 30 cm high, with a cylindrical oxygen filling and binding opening at the top, extending 20 cm in length and with an opening diameter of 25 cm, and integrating a micro water quality sensor interface.

[0012] Furthermore, the multifunctional composite inner liner bag is soaked before use to load the inner wall with probiotic capsules and ammonia nitrogen adsorption hydrogel.

[0013] Furthermore, the intelligent outer casing system comprises: an outer casing that is a phase change temperature-controlled foam box, with a rectangular structure measuring 60cm in length, 40cm in width, and 40cm in height, made of high-density EPS foam with a wall thickness of 5cm; paraffin-based composite material is injected into the interlayer of the casing to achieve a phase change temperature of 25±1℃, thereby realizing active temperature control; a spring-silicone composite shock-absorbing module is designed at the bottom of the casing; and a micro control unit is embedded in the lid to receive water quality sensor data from the inner liner bag.

[0014] Furthermore, the supporting components include water-soluble fiber cable ties that can be slowly degraded in seawater, and a slow-release functional package containing oxygenating tablets, solid pH stabilizers, and ion regulators.

[0015] Furthermore, the emergency treatment kit includes emergency equipment such as physical cooling ice packs and portable oxygen cylinders.

[0016] Another objective of this invention is to provide a method for long-distance water transport of berried mud crabs based on dynamic regulation of the microenvironment water body, comprising:

[0017] Step 1: Microscopic examination and screening during embryonic development

[0018] The embryo is in the stage between the blastocyst stage and the intramembranous zoea stage, when it is more firmly attached and more tolerant, making it the "golden window" for long-distance transportation. Therefore, before transportation, a small number of embryos should be carefully taken from the abdomen of the berried crab and its developmental stage should be observed under a microscope. Berried crabs whose embryos are in the stage between the blastocyst stage and the intramembranous zoea stage should be selected as the transportation targets.

[0019] Step 2, Pre-treatment of parent plants before transportation

[0020] Due to the limited internal space and water volume of the transportation system, the excrement of berried crabs can easily lead to water quality deterioration. Therefore, candidate berried crabs need to be starved for 24 hours before transportation. On the day of transportation, vitamin C (1.5g / cubic meter of water) can be sprinkled into the holding tank before capture to reduce the stress response caused by the capture operation. Then, carefully scoop the berried crabs to be transported with a ladle or basin and disinfect them by soaking them in seawater containing 2 ppm formaldehyde for 15 minutes, and then transfer them to clean seawater for 15 minutes to remove residual formaldehyde from their body surface and body. Finally, use water-soluble fiber ties to tie their two chelipeds to prevent them from breaking the inner bag when they are put into it.

[0021] Step 3, Transportation System Assembly

[0022] Seawater that has been disinfected and dechlorinated is injected into the inner bag, with the water depth controlled at about 15-20cm. The slow-release function pack is then added. The tied-up berried crabs are carefully placed into the inner bag, and pure oxygen is added into the bag until it expands to a moderate degree. The bag opening is then sealed, and the integrated monitoring module is activated. The inner bag is then placed into the outer box, and the box lid is fastened, allowing the system to enter the self-test and steady-state control mode.

[0023] Step 4, Dynamic Management of the Transportation Process

[0024] The transport vehicle should be equipped with GPS and temperature monitoring equipment, with the compartment temperature preset to 24-26℃; the monitoring module records and wirelessly transmits the compartment temperature, dissolved oxygen, and pH data to the monitoring platform every 30 minutes; when the data is abnormal, the system will automatically alarm, and accompanying personnel can use the equipment in the emergency treatment kit to increase oxygen or adjust the temperature in a timely manner according to the specific situation.

[0025] Step 5: Post-transport processing and aquaculture management

[0026] After the berried crabs arrive at their destination, immerse the entire inner bag in a pre-prepared transition tank (with water quality similar to the transport water). Cut open the non-furry area at the top of the inner bag with scissors to allow the berried crabs to crawl out on their own. Remove any ribbons that have not completely dissolved or have softened. Once the berried crabs have recovered in the transition tank, transfer them to a dark, oxygenated hatching tank for further rearing.

[0027] Based on the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions protected by this invention are as follows:

[0028] This invention's system comprises a hydrophilic canvas inner bag with controllable permeability and a nested temperature-controlled foam outer box, combined with an embedded multi-parameter water environment monitoring module and a feedback oxygen supply device, forming a stable transport microenvironment. The operational process includes precise identification of the embryonic development stage, pre-transport stress pretreatment, gradient water quality stabilization treatment, and post-transport recovery. This invention establishes for the first time a three-dimensional synergistic protection mechanism of "embryo-mother-water environment" during the transport of berried mud crabs. Through several technological innovations, such as optimized mechanical vibration buffering design, active suppression of temperature fluctuations, in-situ adsorption of metabolic waste, and stabilization of the embryo attachment interface, it solves the problem of high embryo and parent crab mortality rates caused by vibration, temperature changes, insufficient dissolved oxygen, and water quality deterioration during long-distance transport of berried mud crabs. Using this system and operating method, after a 12-hour, 900-kilometer transport, the survival rate of berried mud crab parents reached 100%, and the embryo hatching success rate upon arrival was as high as 98%. This achieves safe and efficient long-distance cross-regional transport of mud crab germplasm resources, and has significant application value for the high-quality development of the mud crab seed industry.

[0029] This invention abandons the traditional "simple container + oxygenation" approach, pioneering the concept of a "bionic steady-state transport cabin." It absorbs broadband vibrations through a multi-level buffer and damping structure, reducing or even eliminating the mechanical stimulation of parent crabs and embryos during transport. An active temperature control layer using phase change material ensures a stable aquatic microenvironment throughout transport. In-situ water purification is achieved by loading beneficial microorganisms and adsorbents onto the hydrophilic inner liner, avoiding a vicious cycle of "toxicity-hypoxia" caused by accumulated metabolic waste and decreased dissolved oxygen. Water-soluble cable ties are used to secure the chelipeds, reducing secondary stress caused by untying them upon arrival. A transport time window model based on embryonic development timelines enables precise planning of transport activities. This invention fills the gap in specialized long-distance live transport technology for berried mud crabs, and the technical solution is highly species-specific, environmentally controllable, and physiologically friendly.

[0030] This invention achieves significant progress in environmental stability, biosafety, and long-distance adaptability by systematically integrating functions and hierarchically designing the transportation structure, compared to existing transportation methods that rely solely on ordinary water tanks, insulated boxes, or simple cushioning packaging.

[0031] Firstly, the multifunctional composite inner liner bag adopts a three-layer composite fabric structure, simultaneously achieving load-bearing, cushioning, waterproofing, and bio-friendly functions within the same carrier. The inner layer, made of ultra-fine fiber hydrophilic fleece, simulates a natural attachment matrix, allowing the berried crab's egg mass to adhere stably and remain moist, significantly reducing the risk of detachment and damage during transportation caused by slippage, friction, and alternating wet and dry conditions. The middle high-elasticity silicone damping layer effectively absorbs high-frequency micro-vibrations caused by vehicle vibration, loading and unloading impacts, and uneven road surfaces, preventing mechanical stress from directly acting on the individual and egg mass. The outer high-strength waterproof canvas ensures the overall structural strength and sealing, preventing leakage and damage. This multi-layered synergistic structure achieves unified biological and mechanical cushioning within the same carrier for the first time, significantly improving transportation safety.

[0032] Secondly, the intelligent outer casing system actively isolates itself from external environmental disturbances by introducing a phase change temperature control mechanism and a shock-absorbing structure. The paraffin-based composite material inside the interlayer keeps the casing temperature stably locked at around 25°C, protecting the water inside from diurnal temperature variations and climate changes, thus preventing abnormal embryonic development or stress-induced death due to sudden temperature changes. The composite shock-absorbing module composed of bottom springs and silicone further attenuates low-frequency impacts and large-amplitude vibrations, forming a two-stage buffer system with the inner liner damping layer. This ensures the stability of the microenvironment even under long-distance, multi-condition transportation conditions, which is difficult to achieve with traditional single buffer or single insulation structures.

[0033] Furthermore, this invention achieves real-time sensing and dynamic control of the transportation microenvironment through the linkage of a miniature water quality detector and a control and display panel, transforming the transportation process from passive protection to active regulation. When dissolved oxygen decreases, pH becomes abnormal, or temperature deviates, timely oxygen supplementation, water replacement, or emergency treatment measures can be taken to prevent problems from accumulating to an irreversible stage, fundamentally improving survival rate and success rate.

[0034] In summary, this invention achieves a high degree of integration of buffering, temperature control, biofriendliness, and monitoring and regulation in its structure, upgrading the transportation system from a single container to a controllable micro-ecosystem. It is significantly superior to existing technologies in terms of stability, safety, and intelligence, and has significant technological progress and practical value. Attached Figure Description

[0035] Figure 1 This is a structural diagram of a long-distance water transport system for berried mud crabs based on dynamic regulation of microenvironment water bodies, provided in an embodiment of the present invention.

[0036] Figure 2 This is a cross-sectional view of the inner liner bag provided in an embodiment of the present invention.

[0037] Figure 3 This is a diagram of the thermal insulation and earthquake-resistant outer casing system provided in an embodiment of the present invention.

[0038] Figure 4This is a cross-sectional view of the outer box provided in an embodiment of the present invention.

[0039] Figure 5 This is a diagram of the outer casing with a CNC system provided in an embodiment of the present invention.

[0040] Figure 6 This is a flowchart of a method for long-distance water transport of berried mud crabs based on dynamic regulation of microenvironment water bodies, provided in an embodiment of the present invention.

[0041] In the picture: 1. Cylindrical oxygen filling and binding port; 2. Composite inner bag made of three-layer composite fabric; 3. Outer high-strength waterproof canvas; 4. Middle high-elasticity silicone damping layer; 5. Inner microfiber hydrophilic fleece; 6. Paraffin composite material filled in the foam box; 7. Silicone; 8. Spring; 9. Miniature water quality detector; 10. Control and display panel. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0043] like Figure 1 As shown in the figure, an embodiment of the present invention provides a long-distance water transport system for berried mud crabs based on dynamic regulation of microenvironment water bodies, comprising:

[0044] Multifunctional composite inner bag, intelligent outer box system, supporting components, emergency handling kit;

[0045] The multifunctional composite inner bag includes a cylindrical oxygen filling and binding opening 1 and a composite inner bag 2 made of three layers of composite fabric;

[0046] The composite inner bag 2 is made of three-layer composite fabric. The outer layer is high-strength waterproof canvas 3; the middle layer of the composite inner bag 2 is a high-elasticity silicone damping layer 4; the inner layer of the composite inner bag 2 is a microfiber hydrophilic fleece 5.

[0047] The intelligent outer casing system includes paraffin-based composite material 6, silicone 7, spring 8, miniature water quality detector 9, and control display panel 10, all filled inside a foam box;

[0048] Silicone 7 and spring 8 constitute a spring-silicone composite shock absorption module;

[0049] The miniature water quality detector 9 is connected to the control and display panel 10 via a circuit.

[0050] like Figure 2As shown, the multifunctional composite inner bag provided in this embodiment of the invention is made of three layers of composite fabric; the inner layer is a microfiber hydrophilic fleece, which simulates the texture of natural seaweed attachment substrate to reduce embryo friction and mechanical damage; the middle layer is a high-elasticity silicone damping layer (1-2mm thick) to absorb high-frequency micro-vibrations; the outer layer is a high-strength waterproof canvas; the bag body is a cuboid, 50 cm long, 30 cm wide, and 30 cm high, with a cylindrical oxygen filling and binding opening at the top, extending 20 cm in length and with an opening diameter of 25 cm, and integrating a miniature water quality sensor interface (for connecting pH, temperature, and dissolved oxygen probes).

[0051] The multifunctional composite inner liner bag provided in this embodiment of the invention: before use, the inner wall is loaded with probiotic capsules (such as lactic acid bacteria, Bacillus, yeast, Clostridium butyricum, etc.) and ammonia nitrogen adsorption hydrogel by soaking treatment.

[0052] like Figure 3 , Figure 4 , Figure 5 As shown, the intelligent outer box system provided in this embodiment of the invention comprises: an outer box that is a phase change temperature-controlled foam box, the box body being a cuboid structure with a length of 60cm, a width of 40cm, and a height of 40cm, made of high-density EPS foam with a wall thickness of 5cm; paraffin-based composite material is injected into the interlayer of the box body to make its phase change temperature point 25±1℃, so as to achieve active temperature control; a spring-silicone composite shock absorption module is designed at the bottom of the box body; a micro control unit is embedded in the box cover to receive water quality sensor data from the inner liner bag.

[0053] The supporting components provided in this embodiment of the invention include water-soluble fiber cable ties that can be slowly degraded in seawater, and a slow-release functional package containing oxygenating tablets, solid pH stabilizers, and ion regulators.

[0054] The emergency treatment kit provided in this embodiment of the invention includes emergency equipment such as physical cooling ice packs and portable oxygen cylinders.

[0055] This invention provides a long-distance water-carrying transport system for berried mud crabs based on dynamic microenvironment water body control. The system comprises a multifunctional composite inner bag, an intelligent outer casing system, supporting components, and an emergency treatment kit. The multifunctional composite inner bag directly carries the berried mud crabs and their surrounding water, while the intelligent outer casing system buffers and controls temperature, vibration, and water quality changes. Together, they create a stable and controllable transport microenvironment.

[0056] like Figure 2As shown, the multifunctional composite inner bag 2 adopts a three-layer composite fabric structure. The outer layer is a high-strength waterproof canvas 3, which is used to withstand external impacts during transportation and is waterproof and tear-resistant. The middle layer is a high-elasticity silicone damping layer 4, with a thickness of 1-2mm, which is used to absorb high-frequency micro-vibrations and impact energy generated during transportation. The inner layer is an ultra-fine fiber hydrophilic fleece 5, whose soft, hydrophilic, and microporous surface structure simulates the feel of natural seaweed or benthic attachment substrates, allowing the egg masses of berried crabs to attach stably and remain moist, thereby reducing the risk of friction, pressure, and detachment. The bag body has a rectangular structure, with a cylindrical oxygen filling and binding port 1 at the top. This oxygen filling port can be used for artificial or automatic oxygen replenishment, and can also serve as a sealing and hanging structure. It also integrates a water quality sensor interface for connecting pH, temperature, and dissolved oxygen probes.

[0057] The inner liner is soaked before use to load the inner wall with probiotic capsules and ammonia nitrogen adsorption hydrogel, which can adsorb and buffer metabolites such as ammonia nitrogen and nitrite during transportation, and regulate the stability of the microecology through probiotics.

[0058] like Figure 3 , Figure 4 As shown, the intelligent outer casing system includes a phase change temperature-controlled foam casing body, a layer of paraffin-based composite material 6 filled with layers of material, a bottom spring-silicone composite shock-absorbing module, and a control and display unit. The spring-silicone composite shock-absorbing module consists of silicone 7 and springs 8. The outer casing body is a high-density EPS foam cuboid structure, with paraffin-based composite material filling its layers to stabilize the phase change temperature at 25±1℃. This allows the internal temperature to remain constant through phase change heat absorption and release when the ambient temperature fluctuates. The bottom spring-silicone composite shock-absorbing module buffers and isolates impacts from vehicle movement, loading and unloading, and uneven road surfaces.

[0059] like Figure 5 As shown, a miniature water quality detector 9 is installed inside the inner liner bag to detect parameters such as water temperature, dissolved oxygen, and pH in real time. It is connected to the control and display panel 10 embedded in the cover through a circuit line to realize real-time display and early warning of water quality status.

[0060] During transportation, the berried mud crabs are placed inside a multifunctional composite inner bag, with the egg clusters naturally attached to the hydrophilic velvet layer, maintaining a stable and moist state. The inner bag effectively attenuates vibration and impact through a damping layer, while the outer waterproof canvas ensures structural strength and airtightness. After the inner bag is placed inside the intelligent outer box, the phase change material in the outer box locks the temperature at approximately 25°C, which is suitable for incubation and survival, through heat absorption or release, thereby avoiding temperature stress caused by diurnal or climatic changes.

[0061] When the water quality changes during transportation due to metabolism or external factors, the miniature water quality detector collects parameters in real time and feeds them back to the control panel. The operator can then adjust the oxygen supply, water exchange, or add emergency regulators based on the displayed results, achieving dynamic control of the microenvironment. Simultaneously, the bottom shock-absorbing module and the inner liner damping layer form a two-stage buffer system, allowing mechanical impacts to be gradually attenuated within the multi-layered structure, effectively protecting the egg mass and mother crab from mechanical damage.

[0062] This invention constructs a stable, low-stress, and high-survival-rate aquatic transport microenvironment during long-distance transportation through a synergistic mechanism of structural buffering, phase change temperature control, microenvironment regulation, and real-time monitoring, thereby achieving the goal of safe, efficient, and controllable long-distance transportation of berried mud crabs.

[0063] like Figure 6 As shown in the figure, an embodiment of the present invention provides a method for long-distance water transport of berried mud crabs based on dynamic regulation of microenvironment water bodies, comprising:

[0064] S101, Microscopic examination and screening during embryonic development stages

[0065] The embryo is in the stage between the blastocyst stage and the intramembranous zoea stage, when it is more firmly attached and more tolerant, making it the "golden window" for long-distance transportation. Therefore, before transportation, a small number of embryos should be carefully taken from the abdomen of the berried crab and its developmental stage should be observed under a microscope. Berried crabs whose embryos are in the stage between the blastocyst stage and the intramembranous zoea stage should be selected as the transportation targets.

[0066] S102, Pre-treatment of parent plants before transportation

[0067] Due to the limited internal space and water volume of the transportation system, the excrement of berried crabs can easily lead to water quality deterioration. Therefore, candidate berried crabs need to be starved for 24 hours before transportation. On the day of transportation, vitamin C (1.5g / cubic meter of water) can be sprinkled into the holding tank before capture to reduce the stress response caused by the capture operation. Then, carefully scoop the berried crabs to be transported with a ladle or basin and disinfect them by soaking them in seawater containing 2 ppm formaldehyde for 15 minutes, and then transfer them to clean seawater for 15 minutes to remove residual formaldehyde from their body surface and body. Finally, use water-soluble fiber ties to tie their two chelipeds to prevent them from breaking the inner bag when they are put into it.

[0068] S103, Transportation System Assembly

[0069] Seawater that has been disinfected and dechlorinated is injected into the inner bag, with the water depth controlled at about 15-20cm. The slow-release function pack is then added. The tied-up berried crabs are carefully placed into the inner bag, and pure oxygen is added into the bag until it expands to a moderate degree. The bag opening is then sealed, and the integrated monitoring module is activated. The inner bag is then placed into the outer box, and the box lid is fastened, allowing the system to enter the self-test and steady-state control mode.

[0070] S104, Dynamic Management of Transportation Process

[0071] The transport vehicle should be equipped with GPS and temperature monitoring equipment, with the compartment temperature preset to 24-26℃; the monitoring module records and wirelessly transmits the compartment temperature, dissolved oxygen, and pH data to the monitoring platform every 30 minutes; when the data is abnormal, the system will automatically alarm, and accompanying personnel can use the equipment in the emergency treatment kit to increase oxygen or adjust the temperature in a timely manner according to the specific situation.

[0072] S105, Post-arrival processing and aquaculture management

[0073] After the berried crabs arrive at their destination, immerse the entire inner bag in a pre-prepared transition tank (with water quality similar to the transport water). Cut open the non-furry area at the top of the inner bag with scissors to allow the berried crabs to crawl out on their own. Remove any ribbons that have not completely dissolved or have softened. Once the berried crabs have recovered in the transition tank, transfer them to a dark, oxygenated hatching tank for further rearing.

[0074] Specific implementation of the present invention:

[0075] On May 23, 2025, using the water-carrying transportation system and method of this invention, 50 berried mud crabs at different developmental stages were transported from the Ninghai Base of the East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Zhejiang Province, over a distance of approximately 900 kilometers and 12 hours, safely arriving at a mud crab breeding farm in Rizhao City, Shandong Province. Upon arrival, all parent crabs were in normal vitality. During subsequent rearing, except for one berried mud crab that suffered a late-stage embryonic miscarriage due to accidental drop upon arrival, all other individuals successfully hatched healthy zoea larvae. Details are shown in Table 1.

[0076] Table 1. Long-distance transportation of berried mud crabs

[0077]

[0078] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications, equivalent substitutions, and improvements made by those skilled in the art within the scope of the technology disclosed in the present invention, and within the spirit and principles of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A long-distance water-carrying transport system for berried mud crabs based on dynamic regulation of microenvironment water bodies, characterized in that, The system constructs a closed microenvironment within a small body of water and synergistically regulates the physical buffering, biological homeostasis, and chemical homeostasis within this microenvironment to maintain the stability of the embryonic development environment of berried mud crabs during transportation. The system includes an inner bag and an outer box, wherein: The inner liner is used to create an aquatic microenvironment similar to the attachment environment of berried crab embryos, and to reduce the mechanical stress on the embryos through a hydrophilic buffer interface. The outer casing is used to stabilize the temperature and isolate the mechanical vibration of the space where the inner liner bag is located. The system achieves dynamic stability of the embryonic development microenvironment through the synergistic effect of the inner liner bag regulating the biochemical state of the water and the outer box isolating external disturbances, thereby reducing transportation stress and improving the survival rate and embryo integrity rate of berried crabs.

2. The system as described in claim 1, characterized in that, The inner bag has a hydrophilic buffer interface facing the body surface of the berried crab. This interface forms a low-shear contact layer when the water is disturbed, thereby reducing the relative slip velocity between the embryo and the interface and reducing mechanical friction damage.

3. The system as described in claim 1, characterized in that, The inner liner is equipped with a biological homeostasis regulating medium, which is used to slowly release beneficial microorganisms and adsorb metabolic waste, thereby inhibiting the accumulation of harmful substances in the water and stabilizing the biochemical state of the water.

4. A microenvironment synergistic stabilization device for long-distance water transport of berried mud crabs, characterized in that, The device includes a flexible water body support unit and an external disturbance isolation unit. The flexible water body support unit forms a closed water space for accommodating berried mud crabs and absorbs high-frequency mechanical disturbances from the outside through a flexible damping structure. The external disturbance isolation unit buffers temperature fluctuations and isolates vibrations from the flexible water body support unit. The two units work together to form a multi-level buffer link to reduce the intensity of mechanical shocks and temperature fluctuations transmitted to the water microenvironment.

5. The apparatus as described in claim 4, characterized in that, The flexible water-bearing unit forms a gradient damping structure by stacking multiple layers of materials, so that the external impact energy is gradually attenuated before being transmitted to the water.

6. The apparatus as claimed in claim 4, characterized in that, The external disturbance isolation unit absorbs the heat changes caused by ambient temperature fluctuations through a phase change heat buffering mechanism, thereby reducing the rate of change in water temperature.

7. A method for long-distance water transport of berried mud crabs using the system described in any one of claims 1 to 6, characterized in that, The method reduces transport stress by constructing and maintaining a stable microenvironment in the water body throughout the transport process, and includes the following steps: Step 1: Screening is performed on the embryonic development stages of berried mud crabs, and individuals with firmly attached embryos and high tolerance are selected for transportation; Step 2: Implement excretion load reduction treatment on the selected berried mud crabs to reduce the rate of water pollution during transportation; Step 3: Place the treated berried mud crabs into a closed water space with a microenvironment regulation mechanism, and initialize the gas and water quality status of the water space. Step 4: Maintain the physical, biological, and chemical stability of the aquatic microenvironment throughout the transportation process; Step 5: After arrival, allow the berried mud crabs to recover and transition in a gradually changing aquatic environment.

8. The method as described in claim 7, characterized in that, In step 4, the stability of the microenvironment is determined by monitoring the changing trends of dissolved oxygen, pH, and temperature in the water, and compensation adjustments are implemented when the temperature deviates from the stable range.

9. The method as described in claim 7, characterized in that, In step 5, a gradual water change method is used to allow the berried mud crabs to gradually adapt to the destination water environment in order to avoid stress reactions caused by sudden changes in water quality.

10. The method as described in claim 7, characterized in that, The compensation regulation includes at least two of the following: increasing the dissolved oxygen content in the water, reducing the rate of change in water temperature, and reducing the concentration of metabolic waste.

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