Plant silicon water capable of improving lipid metabolism and preparation method of plant silicon water

Abstract

The invention belongs to the technical field of functional food, and particularly relates to plant silicon water capable of improving lipid metabolism and a preparation method of the plant silicon water. The preparation method of the plant silicon water comprises the following steps: S1, providing a fumed silica precursor; s2, placing the fumed silica precursor in a reaction device to obtain silicon-containing gas; s3, the silicon-containing gas is guided into an oxygen-enriched combustion chamber for combustion purification, and a purified silicon gas phase product is obtained; s4, introducing the purified silicon gas-phase product into pure water for dissolution and crystallization to obtain a plant silicon water crude product; and S5, performing component quantification and regulation on the plant silicon water crude product. According to the invention, metasilicic acid and silicon dioxide are combined with a set of complete high-temperature gas-phase reaction, purification, dissolution crystallization and feedback regulation preparation process according to a specific component ratio. The silicon is ensured to stably exist in water in the form of water-soluble silicon dioxide, and the problems of poor water solubility and unstable components of common silicon dioxide are solved.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology, and in particular relates to a plant-based silicon water that improves lipid metabolism and its preparation method. Background Technology

[0002] Cardiovascular disease is one of the leading causes of death worldwide. Hypercholesterolemia is a key risk factor for cardiovascular disease. While widely used cholesterol-lowering drugs (such as statins and ezetimibe) have some efficacy, long-term use may cause adverse reactions such as muscle toxicity and new-onset diabetes, affecting patient adherence. Therefore, developing safe and effective dietary intervention products or functional foods is of great significance.

[0003] Silica, especially its water-soluble form, has attracted research attention in recent years due to its high specific surface area and strong adsorption capacity. Existing literature suggests that certain forms of silica or silicon-containing substances may have positive effects on lipid metabolism. For example, studies have reported that fumed silica can reduce cholesterol levels in rats on a high-cholesterol diet; metasilicic acid is thought to potentially exert an anti-atherosclerotic effect by regulating the activity of lipid metabolism-related enzymes. However, the specific mechanism by which water-soluble silica lowers cholesterol is not yet fully understood.

[0004] Currently, there is a lack of water-soluble silica products on the market with stable active ingredients that can lower cholesterol. Summary of the Invention

[0005] The main objective of this invention is to provide a plant-based silicon water that improves lipid metabolism and its preparation method, thereby overcoming the shortcomings of the prior art.

[0006] To achieve the aforementioned objectives, the technical solution adopted by this invention includes: According to a first aspect of the present invention, a method for preparing plant-based silicon water that improves lipid metabolism is provided, comprising the following steps: S1. Provides fumed silica precursor; S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1600°C to 1800°C to obtain silicon-containing gas. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification to obtain purified silicon gaseous products; S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled between 20°C and 40°C to obtain crude plant silicon water. S5. The components of the crude plant silica water are quantified and controlled to ensure that the concentration of metasilicic acid in the final product is stable at 14.0-16.0 g / L and the concentration of silica is stable at 9.5-11.5 g / L.

[0007] Furthermore, in step S5, the component quantification includes: determining the metasilicic acid content using silicomolybdenum yellow spectroscopy, and determining the silicon dioxide content using inductively coupled plasma atomic emission spectrometry.

[0008] Furthermore, based on the quantitative determination results of the components in step S5, the amount of fumed silica precursor fed in step S1 is adjusted in reverse, and / or the heating temperature and time in step S2 are adjusted, so as to achieve feedback control of the composition of the crude plant silica water product.

[0009] Furthermore, in step S3, after the combustion purification, a deacidification step is also included, in which nitrogen is used to purge the purified silicon gaseous product with a carrier flow until the pH value of the product stabilizes at 4-6.

[0010] Furthermore, the fumed silica precursor is silicon tetrachloride.

[0011] Furthermore, the decomposition reaction of the fumed silica precursor in step S2 is a gas-phase hydrolysis reaction carried out in the presence of oxygen and hydrogen.

[0012] According to a second aspect of the present invention, a plant-based silica water prepared by any of the above-described preparation methods is provided, comprising water-soluble silica, wherein the water-soluble silica is composed of metasilicic acid and silica, wherein the concentration of metasilicic acid is 14.0-16.0 g / L and the concentration of silica is 9.5-11.5 g / L.

[0013] Furthermore, the acute oral toxicity LD50 of the plant silicon water is greater than 5000 mg / kg body weight.

[0014] Furthermore, when the concentration of the plant silicon in water is 7000 ppm, the in vitro adsorption rate of cholesterol is not less than 30%.

[0015] Furthermore, the concentration of metasilicic acid is 14.9 g / L, and the concentration of silicon dioxide is 10.4 g / L.

[0016] Compared with the prior art, the advantages of the present invention include: This invention provides a plant-based silicon water that improves lipid metabolism and its preparation method. The invention combines metasilicic acid and silicon dioxide in a specific ratio with a complete high-temperature gas-phase reaction, purification, dissolution and crystallization, and feedback control process. This process ensures that silicon exists stably in water as water-soluble silicon dioxide, overcoming the problems of poor water solubility and unstable composition of ordinary silicon dioxide. Furthermore, the quantitative component analysis and feedback control of process parameters guarantee a high degree of consistency in product quality between batches. Attached Figure Description

[0017] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 The cholesterol content is determined by adding different concentrations of water-soluble silica; Figure 2 Different dosages of water-soluble silica were added; Figure 3 The plasma TC levels in hamsters at weeks 0 and 6; Figure 4 Plasma TG levels in hamsters at weeks 0 and 6; Figure 5 The plasma HDL-C levels in hamsters at weeks 0 and 6; Figure 6 The plasma Non-HDL-C levels in hamsters at weeks 0 and 6; Figure 7 The plasma HDL-C / TC ratio of hamsters at week 0 and week 6; Figure 8 The ratio of Non-HDL-C to HDL-C in hamster plasma at week 0 and week 6. Detailed Implementation

[0018] In view of the shortcomings of the prior art, the inventors of this invention, through long-term research and extensive practice, have proposed the technical solution of this invention. The following will further explain and illustrate this technical solution, its implementation process, and its principles.

[0019] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0020] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials used in the following embodiments are commercially available.

[0021] This invention provides a method for preparing plant-based silicon water that improves lipid metabolism, comprising the following steps: S1. Provide a fumed silica precursor; in some embodiments, the fumed silica precursor is silicon tetrachloride (SiCl4). S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1600°C to 1800°C to obtain silicon-containing gas; the decomposition is a gas-phase hydrolysis reaction carried out in the presence of oxygen and hydrogen, and the reaction raw materials are SiCl4, O2, and H2. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification. After the combustion purification, a deacidification step is further included by using nitrogen to purge the purified silicon gas phase product until the pH value of the product stabilizes at 4-6; a purified silicon gas phase product is obtained. This step helps to remove acidic impurities and improve the purity and stability of the product. S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled between 20°C and 40°C to obtain crude plant silicon water. S5. The crude plant silicate water is subjected to component quantification and regulation to ensure that the metasilicic acid concentration in the final product is stabilized at 14.0-16.0 g / L and the silica concentration is stabilized at 9.5-11.5 g / L. The component quantification includes: determining the metasilicic acid content using the silicomolybdenum yellow spectrophotometry method (referring to the relevant standard GB 8538-2022), and determining the silica content using inductively coupled plasma atomic emission spectrometry.

[0022] In some embodiments, based on the quantitative determination results of the components in step S5, the amount of fumed silica precursor fed in step S1 is adjusted in reverse, and / or the heating temperature and time in step S2 are adjusted, so as to achieve feedback control of the composition of the crude plant silica water and ensure the stability of the product composition between batches.

[0023] This invention provides a plant-based silica water prepared by any of the above-described methods. The plant-based silica water contains water-soluble silica, which is composed of metasilicic acid and silica, wherein the concentration of metasilicic acid is 14.0-16.0 g / L and the concentration of silica is 9.5-11.5 g / L.

[0024] Preferably, the acute oral toxicity LD50 of the plant silicon water is greater than 5000 mg / kg body weight, which is practically non-toxic and has extremely high food safety. Furthermore, the silicon dioxide component does not enter the bloodstream and is excreted through feces, providing a solid safety basis for its use as a functional food or raw material for long-term consumption.

[0025] Preferably, when the concentration of the plant silica in the water is 7000 ppm, the in vitro adsorption rate of cholesterol is not less than 30%, indicating that it has good physical adsorption capacity for cholesterol. The concentration of the metasilicic acid is 14.9 g / L, and the concentration of the silica is 10.4 g / L.

[0026] To better understand the technical solution of the present invention, the following detailed discussion is provided in conjunction with specific embodiments. Example 1

[0027] This embodiment provides a method for preparing plant-based silicon water that improves lipid metabolism, including the following steps: S1. Provide a fumed silica precursor; the fumed silica precursor is SiCl4; S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1600°C to obtain silicon-containing gas. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification. Then, nitrogen is used to purge the purified silicon gaseous product until the pH value of the product stabilizes at 4; thus, the purified silicon gaseous product is obtained. S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled at 20°C to obtain crude plant silicon water. S5. The components of the crude plant silicate water are quantified and controlled to ensure that the concentration of metasilicic acid in the final product is stable at 14.0 g / L and the concentration of silica is stable at 9.5 g / L. Example 2

[0028] This embodiment provides a method for preparing plant-based silicon water that improves lipid metabolism, including the following steps: S1. Provide a fumed silica precursor; the fumed silica precursor is SiCl4; S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1800°C to obtain silicon-containing gas. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification. Then, nitrogen is used to purge the purified silicon gaseous product until the pH value of the product stabilizes at 6; thus, the purified silicon gaseous product is obtained. S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled between 20°C and 40°C to obtain crude plant silicon water. S5. The components of the crude plant silica water are quantified and controlled to ensure that the concentration of metasilicic acid in the final product is stable at 16.0 g / L and the concentration of silica is stable at 11.5 g / L. Example 3

[0029] This embodiment provides a method for preparing plant-based silicon water that improves lipid metabolism, including the following steps: S1. Provide a fumed silica precursor; the fumed silica precursor is SiCl4; S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1700°C to obtain silicon-containing gas. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification. Then, nitrogen is used to purge the purified silicon gaseous product until the pH value of the product stabilizes at 5; thus, the purified silicon gaseous product is obtained. S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled at 30°C to obtain crude plant silicon water. S5. The components of the crude plant silicate water are quantified and controlled to ensure that the concentration of metasilicic acid in the final product is stable at 14.9 g / L and the concentration of silica is stable at 10.4 g / L.

[0030] Experiment 1 Raw material preparation: Silicon tetrachloride (SiCl4, purity ≥99.9%) is provided as a precursor for fumed silica. High-purity oxygen and hydrogen are also prepared.

[0031] High-temperature decomposition and gas-phase reaction: SiCl4 vapor, oxygen, and hydrogen are introduced into a quartz tube reactor at a preset molar ratio. The temperature at the center of the reaction zone is controlled at 1700℃, allowing the raw materials to undergo gas-phase hydrolysis under these conditions, generating gas-phase products containing nano-silica components.

[0032] Combustion purification and deacidification: The above gaseous product was introduced into a combustion chamber filled with oxygen-enriched air for secondary combustion to completely decompose residual organic impurities and chlorides. High-purity nitrogen was used as the carrier gas to continuously purge the purified gaseous product for 30 minutes for deacidification. A small amount of the product was dissolved in pure water and tested; its pH value remained stable at 5.2.

[0033] Dissolution and crystallization: The deacidified silicon gaseous product is passed into an absorption tank containing high-purity water at a constant temperature of 20°C. The gas flow rate is controlled to ensure sufficient gas-liquid contact, allowing the silicon gas to dissolve and form a stable solution, yielding crude plant silicon water.

[0034] Component quantification: Metasilicic acid determination: Crude plant silicate solution was taken, and its absorbance was measured at a specific wavelength according to the standard procedure of the silicomolybdate yellow spectrophotometric method. Based on the pre-plotted metasilicic acid standard curve, the concentration of metasilicic acid (calculated as H2SiO3) in this batch of crude product was calculated to be 15.2 g / L.

[0035] Silicon dioxide determination: Another sample of crude plant silicon water was taken, diluted appropriately, and the silicon content was determined using inductively coupled plasma optical emission spectrometry (ICP-OES) at a wavelength of 251.611 nm. The silicon dioxide (SiO2) concentration was calculated to be 10.1 g / L using the formula (silicon content × 2.1393).

[0036] Feedback control: The above measurement results show that the concentrations of metasilicic acid (15.2 g / L) and silica (10.1 g / L) are relatively stable. If the measured value of a batch is close to the lower limit of the range, in the next batch of production, the feed rate of SiCl4 in step S1 will be increased by about 5%, or the reaction temperature in step S2 will be increased by 20°C, so that the product concentration returns to the central region of the target range. This feedback control mechanism ensures the consistency of product composition.

[0037] Final product characteristics: The plant-based silicon water produced by the above process showed an LD50 > 5000 mg / kg in an acute oral toxicity test (rat). In vitro adsorption experiments showed that when the product was diluted to a concentration of 7000 ppm, its adsorption rate for cholesterol solution reached 33%.

[0038] Table 2.3 Detection results of metasilicic acid and silica

[0039] Key data demonstrating that the product's composition is clearly defined, stable, and the preparation process is controllable: Table 2.3 (Testing results of silicic acid and silicon dioxide): This table directly provides the precise concentrations of metasilicic acid (14.9 g / L) and silicon dioxide (10.4 g / L) in the plant-based silicon water of this invention. This data serves as the experimental basis for the core component range stated in the claims, directly demonstrating the advantages of this invention's product over prior art with "unclear composition."

[0040] Table 2.4 Statistics on animal mortality in acute oral toxicity tests

[0041] Table 2.5 Changes in body weight of animals in acute oral toxicity test

[0042] Figure 1 The cholesterol content was determined by adding different concentrations of water-soluble silica. Figure 2 Different dosages of water-soluble silica were added; analysis revealed... Figure 1 In the two experiments, the cholesterol levels in the blank control group were 37.90 mg / g and 72.24 mg / g, respectively. The cholesterol level in the group with added water-soluble silica showed a significant decreasing trend with increasing dosage and concentration. Figure 2 As shown, in experiments with different dosages, cholesterol levels decreased by 13.16% in the 0.5 g group, 27.31% in the 1 g group, and 30.13% in the 2 g group, with the highest dosage showing a decrease of 18.57% compared to the lowest dosage. Among different concentration gradients, 7000 ppm of water-soluble silica showed a significant effect, decreasing cholesterol levels by 35.27%. The cholesterol-lowering effect of water-soluble silica may be achieved through physical adsorption. Due to its large specific surface area and strong adsorption capacity, cholesterol molecules in the mixture are adsorbed onto its surface by the high adsorption capacity and thus removed from the mixture.

[0043] Figure 3 The plasma TC levels in hamsters at weeks 0 and 6 were measured. Figure 4 The plasma TG levels of hamsters at weeks 0 and 6 were measured. Figure 5 The plasma HDL-C levels in hamsters at weeks 0 and 6 were measured. Figure 6 The plasma Non-HDL-C levels in hamsters at weeks 0 and 6 were measured. Figure 7 The HDL-C / TC ratio in hamster plasma at weeks 0 and 6. Figure 8 The ratio of Non-HDL-C to HDL-C in hamster plasma at week 0 and week 6.

[0044] Total cholesterol (TC) in plasma reflects the total amount of cholesterol contained in lipid apolipoproteins such as LDL and HDL, and is listed by international guidelines as a primary controllable risk factor for cardiovascular events. Elevated TC concentrations have been proven to increase the probability of CVD. As the main form of neutral lipid storage in the circulatory system, TC is transported peripherally via CM and VLDL. Imbalances in TC metabolism not only affect the efficiency of energy substrate supply but are also significantly associated with the pathological progression of ASCVD. Therefore, controlling blood lipid levels is a crucial aspect of CVD prevention and treatment.

[0045] Figure 3-8 Plasma lipid levels in each group of hamsters at weeks 0 and 6. At week 0, the plasma lipid levels in the five groups were similar with no significant difference. By week 6, compared to the NCD group, the HCD group showed significantly higher plasma lipid levels (p < 0.05): TC (29.5%), TG (64.49%), Non-HDL-C (43.39%), and the Non-HDL-C / HDL-C ratio (38.10%). This demonstrates that the hamster hypercholesterolemia model was successfully established after 6 weeks of high-cholesterol dietary intervention, allowing for further analysis. Figure 3 It was found that at week six, compared with HCD, the TC level decreased by 9.36% in the low-dose water-soluble silica group (p < 0.05) and by 10.03% in the high-dose group (p < 0.05), but the decrease was not significant compared with the cholestyramine positive control group. This indicates that water-soluble silica can inhibit the increase in plasma TC levels in hamsters induced by a high-cholesterol diet.

[0046] Figure 4 It was found that at week six, compared with the HCD group, the ALD group showed a 33.92% decrease in TG levels (p < 0.05), while the AHD group showed a significant 41.85% decrease in TG levels (p < 0.05), and the PCD group showed a 38.60% decrease. The percentage decrease in the AHD group was greater than that in the PCD group. This indicates that water-soluble silica has an inhibitory effect on increasing triglyceride levels in hamsters on a high-cholesterol diet. Figure 5 It was observed that, although there was no significant difference between the AHD and ALD groups and the HCD group in week six, they showed an upward trend, indicating that water-soluble silica had a relatively small impact on hamster plasma HDL-C levels (p > 0.05). Non-HDL-C is a novel lipid assessment indicator, referring to the total cholesterol content in the circulatory system, excluding HDL, including all lipoproteins that contribute to atherosclerosis, such as LDL, VLDL, and their residual particles. The Non-HDL-C / HDL-C ratio is considered an important indicator for assessing cardiovascular disease risk, providing a more comprehensive reflection of atherosclerosis risk and potentially improving the accuracy of disease diagnosis and risk assessment.

[0047] Therefore, the combination is through Figure 6 and 8Compared with the HCD group, plasma non-HDL-C levels in the ALD and AHD groups were reduced by 17.12% and 22.77%, respectively (p < 0.05). In the non-HDL-C / HDL-C ratio, compared with the HCD group, the ALD and AHD groups were reduced by 22.76% and 28.97%, respectively (p < 0.05). These results indicate that dietary water-soluble silica can reduce plasma non-HDL-C levels and the non-HDL-C / HDL-C ratio, potentially reducing the risk of cardiovascular disease.

[0048] The HDL-C / TC ratio is an important indicator for assessing cardiovascular disease risk; a higher HDL-C / TC ratio generally suggests a lower cardiovascular disease risk. Figure 7 The study revealed that the AHD group showed a 21.95% increase compared to the HCD group (p < 0.05), while the low-dose group, although showing an upward trend, did not exhibit a significant difference. This demonstrates that supplementation with water-soluble silica can reduce the risk of CVD.

[0049] The above descriptions are merely some embodiments of the present invention. It should be noted that those skilled in the art can make other modifications and improvements without departing from the inventive concept of the present invention, and these all fall within the protection scope of the present invention.

Claims

1. A method for preparing plant-based silicon water that improves lipid metabolism, characterized in that, Includes the following steps: S1. Provides fumed silica precursor; S2. The fumed silica precursor is placed in a reaction apparatus and heated and decomposed at a temperature of 1600°C to 1800°C to obtain silicon-containing gas. S3. The silicon-containing gas is introduced into an oxygen-enriched combustion chamber for combustion purification to obtain purified silicon gaseous products; S4. The purified silicon gaseous product is passed into pure water for dissolution and crystallization, and the dissolution temperature is controlled between 20°C and 40°C to obtain crude plant silicon water. S5. The components of the crude plant silica water are quantified and controlled to ensure that the concentration of metasilicic acid in the final product is stable at 14.0-16.0 g / L and the concentration of silica is stable at 9.5-11.5 g / L.

2. The preparation method according to claim 1, characterized in that, In step S5, the component quantification includes: determining the metasilicic acid content using the silicomolybdenum yellow spectroscopy method, and determining the silicon dioxide content using inductively coupled plasma atomic emission spectrometry.

3. The preparation method according to claim 2, characterized in that, Based on the quantitative determination results of the components in step S5, the amount of fumed silica precursor fed in step S1 is adjusted in reverse, and / or the heating temperature and time in step S2 are adjusted to achieve feedback control of the composition of the crude plant silica water.

4. The preparation method according to claim 1, characterized in that, In step S3, after the combustion purification, a deacidification step is further included by using nitrogen to purge the purified silicon gas phase product with a carrier flow until the pH value of the product stabilizes at 4-6.

5. The preparation method according to claim 1, characterized in that, The fumed silica precursor is silicon tetrachloride.

6. The preparation method according to claim 1, characterized in that, The decomposition reaction of the fumed silica precursor in step S2 is a gas-phase hydrolysis reaction carried out in the presence of oxygen and hydrogen.

7. A plant-based silicon water prepared by the method according to any one of claims 1-6, characterized in that, It includes water-soluble silica, which is composed of metasilicic acid and silica, wherein the concentration of metasilicic acid is 14.0-16.0 g / L and the concentration of silica is 9.5-11.5 g / L.

8. The plant-based silicon water according to claim 7, characterized in that, The acute oral toxicity LD50 of the plant silicon water is greater than 5000 mg / kg body weight.

9. The plant-based silicon water according to claim 7, characterized in that, When the concentration of the plant silicon in water is 7000 ppm, the in vitro adsorption rate of cholesterol is not less than 30%.

10. The plant-based silicon water according to claim 7, characterized in that, The concentration of metasilicic acid is 14.9 g / L, and the concentration of silicon dioxide is 10.4 g / L.

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