Composition for improving sleep deprivation stress injury

Abstract

The invention discloses a composition for improving sleep deprivation stress injury, and belongs to the field of food health care. The invention provides a composition containing gordon euryale seed, deer sinew collagen and golden camellia, which proves that the composition has a good protective effect in improving stress injury caused by sleep deprivation and can be applied to memory decline and hippocampus injury caused by sleep deficiency or continuous work. Furthermore, the composition is combined with active substances such as tilapia collagen, red ginseng, blueberries, caffeine, taurine and B vitamins, and a series of compositions for improving sleep insufficiency and emergency injury are developed. The composition disclosed by the invention can be developed into foods and health-care products for improving the cognitive function and learning and memory, and has a very good application prospect.

Description

Technical Field

[0001] This invention relates to a composition for improving sleep deprivation stress injury, belonging to the field of food and health care. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, sleep disorders have become an increasingly prominent public health issue. Extensive surveys and research data show that sleep problems are prevalent among adults, with nearly half of adults suffering from varying degrees of sleep disturbance. This population typically experiences shorter average sleep durations than recommended, and often has delayed bedtime. This long-term, widespread sleep deprivation is becoming a "hidden health killer" for young people.

[0003] Sleep deprivation can damage both physiological and psychological functions. Physiologically, chronic sleep deprivation directly leads to memory decline and has toxic effects on the central nervous system, including disordered thinking, slowed reaction time, inattention, disorientation, and impaired learning and memory, severely impacting daytime cognitive function and work efficiency. Multiple studies have shown that compared to those maintaining an average sleep duration (7-8 hours), consistently shorter sleep duration (less than 6 hours) significantly increases the risk of various chronic diseases, including an overall cancer risk increase of over 40%.

[0004] Faced with this serious health challenge, existing intervention methods have significant limitations: In terms of medication, there are currently no universally recognized effective drugs for the stress damage caused by sleep deprivation. Although commonly used sedative-hypnotic drugs can induce sleep, long-term use can easily lead to side effects such as drug dependence, tolerance, and withdrawal reactions. Moreover, they mainly prolong sleep time through forced sedation, and have limited effect on repairing the physiological damage caused by sleep deprivation and improving sleep quality, making them unsuitable long-term solutions.

[0005] In the non-pharmaceutical sector, some functional foods or dietary supplements on the market already exist, primarily based on single or a few natural active ingredients, such as gamma-aminobutyric acid (GABA), melatonin, jujube seed extract, and valerian extract. However, these products generally suffer from limited efficacy and single-target effects. Sleep deprivation stress injury is a complex physiological and pathological process involving multiple pathways and targets. Single ingredients often only act on one or a few aspects, making it difficult to achieve comprehensive improvement in multi-system damage caused by sleep deprivation. This results in unstable or insignificant actual effects, failing to meet the urgent market demand.

[0006] Therefore, there is an urgent need in this field for a solution that is safe, effective, and comprehensive, while ensuring safety and effectiveness and comprehensively improving sleep deprivation stress injury. Summary of the Invention

[0007] To address at least one of the aforementioned problems, this invention provides a novel use for a composition of Euryale ferox, deer tendon collagen, and Camellia chrysantha, namely, the application of this composition in products that improve sleep deprivation stress injury, thereby enhancing the efficacy of "food and medicine of the same origin" and "new resource food" materials in improving sleep deprivation stress injury, thus providing a composition for improving sleep deprivation stress injury.

[0008] Furthermore, based on the composition of foxnut, deer tendon collagen and golden camellia, this invention combines one or more food materials that are both medicinal and edible, which not only improves sleep deprivation stress damage, but also ensures the safety of the raw materials.

[0009] Euryale ferox, also known as "chicken head rice," is an aquatic vegetable belonging to the genus Euryale in the family Nymphaeaceae. It is rich in starch and protein, with nutritional components similar to grains, and can be consumed as a grain. It is also believed to dispel dampness and nourish the stomach. Deer tendon, the dried tendon of the limbs of sika deer, is rich in collagen. Studies have shown that deer tendon collagen can promote cartilage remodeling and maintain bone toughness, and is often used to relieve joint pain and rheumatism-related diseases. Golden camellia, hailed as the "Queen of Teas," contains over 400 nutrients and has unique and remarkable effects on lowering blood sugar, blood pressure, blood lipids, cholesterol, and diabetes and its complications, playing a synergistic and balancing regulatory role. This invention, based on the concept of "principal, assistant, adjuvant, and guide" in traditional Chinese medicine, utilizes Euryale ferox, deer tendon collagen, and golden camellia extract to form a new composition with enhanced effects in improving sleep deprivation stress damage, which is of significant importance in the field of health food products.

[0010] The first objective of this invention is to provide a composition for improving sleep deprivation stress injury, comprising, by weight percentage: 50% to 70% Euryale ferox, 20% to 30% collagen, and 10% to 20% Camellia chrysantha extract.

[0011] In one embodiment of the present invention, the gorgon fruit is pre-treated by crushing to a fineness of 50-200 mesh.

[0012] In one embodiment of the present invention, the collagen is derived from deer tendon.

[0013] In one embodiment of the present invention, the collagen is prepared by using deer tendon as raw material, and then undergoing acid extraction, dialysis, enzymatic hydrolysis, and freeze-drying.

[0014] In one embodiment of the present invention, the method for preparing the Camellia chrysantha extract is as follows: using Camellia chrysantha as raw material, it is obtained by water extraction, filtration, concentration and freeze-drying.

[0015] In one embodiment of the invention, the composition comprises, by weight percentage: 50% to 80%; and any of the following additional components: (1) 20%~50% tilapia collagen; or, (2) 10%~30% red ginseng, 5%~15% blueberry; or, (3) 20%~50% caffeine; or, (4) 20%~50% taurine; or, (5) 20%~50% B vitamins.

[0016] In one embodiment of the present invention, the B vitamins include 10%–30% vitamin B1, 10%–20% vitamin B2, 30%–50% vitamin B3, 10%–20% vitamin B6, and 5%–15% vitamin B6. 12 .

[0017] In one embodiment of the present invention, the red ginseng is red ginseng extract.

[0018] In one embodiment of the present invention, the blueberries are freeze-dried blueberry powder that has been crushed, pasteurized, and freeze-dried.

[0019] A second object of the present invention is to provide a medicament comprising the above-described composition for improving sleep deprivation stress injury.

[0020] A third object of the present invention is to provide a method for preparing the above composition, the method comprising the following steps: (1) Dry the fox nuts and then pulverize them into powder; (2) Deer tendon collagen was prepared by acid extraction, dialysis, enzymatic hydrolysis and freeze drying. (3) A golden camellia extract was prepared by water extraction, filtration, concentration and freeze drying using golden camellia as raw material; (4) Mix 50% to 70% of the Euryale ferox powder from step (1), 20% to 30% of the deer tendon collagen powder from step (2), and 10% to 20% of the Camellia chrysantha extract from step (3) by mass percentage to form a compound extract.

[0021] In one embodiment of the present invention, in step (1), the powder particle size is 50 to 200 mesh.

[0022] In one embodiment of the present invention, in step (2), acid extraction is performed by adding 5 to 15 times the weight of the raw material of deer tendon to pure water and an acidic aqueous solution with a concentration of 3 to 5%, soaking, reflux extraction, filtering, repeatedly extracting the residue, and combining the two extracts. In one embodiment of the present invention, the acidic aqueous solution includes an aqueous solution of citric acid.

[0023] In one embodiment of the present invention, the soaking time is 20-28 hours.

[0024] In one embodiment of the present invention, in step (2), dialysis is performed using a dialysis bag with a molecular weight cutoff of 5 to 50 kDa until the pH value of the protein solution in the bag stabilizes at 6 to 8.

[0025] In one embodiment of the present invention, the dialysis conditions are 20~45°C for 48~120 h.

[0026] In one embodiment of the present invention, in step (2), after enzymatic hydrolysis with protease, the enzyme is inactivated, centrifuged, the supernatant is collected, and then freeze-dried to obtain deer tendon collagen.

[0027] In one embodiment of the present invention, the protease includes one of neutral protease, alkaline protease, pepsin, trypsin, or acidic protease.

[0028] Preferably, the protease is an alkaline protease.

[0029] In one embodiment of the present invention, the amount of protease added is 800~1200 U / g.

[0030] In one embodiment of the present invention, the enzymatic hydrolysis conditions are 37~42℃ for 20~30 h.

[0031] In one embodiment of the present invention, in step (3), 5 to 15 times the weight of the raw material of the golden camellia is added to the tea, and the mixture is refluxed for 1 to 1.5 hours. The residue is then filtered through a 10 to 100 μm membrane and extracted repeatedly. The two extracts are then combined.

[0032] In one embodiment of the present invention, in step (3), the combined supernatant is concentrated under reduced pressure at 50-80°C and 0.1-1 MPa to 10%-30% of its original volume, and then freeze-dried to obtain Camellia chrysantha extract.

[0033] In one embodiment of the invention, the composition comprises, by weight percentage: 50% to 80%; and any of the following additional components: (1) 20%~50% tilapia collagen; or, (2) 10%~30% red ginseng, 5%~15% blueberry; or, (3) 20%~50% caffeine; or, (4) 20%~50% taurine; or, (5) 20%~50% B vitamins.

[0034] In one embodiment of the present invention, the red ginseng is red ginseng extract.

[0035] In one embodiment of the present invention, the blueberries are freeze-dried blueberry powder that has been crushed, pasteurized, and freeze-dried.

[0036] A fourth object of the present invention is to provide the use of the composition in the preparation of products for preventing and / or improving sleep deprivation stress injury.

[0037] In one embodiment of the present invention, the product is a functional food, dietary supplement, or medicine.

[0038] In one embodiment of the present invention, the sleep deprivation stress injury includes depression, bipolar disorder, anxiety, PTSD, schizophrenia, Alzheimer's disease, Parkinson's disease, traumatic brain injury, and cognitive impairment.

[0039] In one embodiment of the present invention, the sleep deprivation stress injury includes cognitive impairment, which includes memory decline, inattention, or decreased learning ability.

[0040] Beneficial effects: This invention, through sleep deprivation experiments, behavioral tests, and physiological and biochemical index detection in mice, confirmed that the combination of Euryale ferox, deer tendon collagen, and Camellia chrysantha has a good protective effect against stress damage caused by sleep deprivation. This essentially demonstrates the application of this composition in improving memory decline and hippocampal damage caused by insufficient sleep or continuous work. Furthermore, this invention has developed a series of compound compositions for improving sleep deprivation stress damage by combining active substances such as tilapia collagen, red ginseng, blueberry, caffeine, taurine, and B vitamins. These compositions for improving sleep deprivation stress damage have the potential to be developed into foods, health products, and drugs to enhance cognitive function and learning memory, showing great promise for application. Attached Figure Description

[0041] Figure 1 This is a behavioral trajectory diagram of a mouse in a water maze, as presented in this invention. Detailed Implementation

[0042] The preferred embodiments of the present invention are described below. It should be understood that the embodiments are for better explanation of the present invention and are not intended to limit the present invention.

[0043] Raw materials used in the examples: Euryale ferox seeds: purchased from Beijing Tongrentang (Group) Co., Ltd., China; Deer tendon: purchased from Beijing Tongrentang (Group) Co., Ltd., China; Golden Camellia: Purchased from Beijing Tongrentang (Group) Co., Ltd., China; Tilapia Collagen: Dezhou Lanli Biotechnology Co., Ltd., with 90% collagen peptides having a relative molecular weight of less than 100,000; Red ginseng extract: Jilin Hanzheng Ginseng Co., Ltd., total ginsenosides ≥3%; Taurine: Qianjiang Yong'an Pharmaceutical Co., Ltd., ≥98.5%; Vitamin B1: Jiangxi Tianxin Pharmaceutical Co., Ltd.; Vitamin B2: Dexing Baiqin Group; Vitamin B3: Jiangxi Tianxin Pharmaceutical Co., Ltd.; Vitamin B6: Jiangxi Tianxin Pharmaceutical Co., Ltd.; Vitamin B 12 Jiangxi Tianxin Pharmaceutical Co., Ltd. Caffeine: CSPC Innovative Pharmaceutical Co., Ltd., ≥98.5%.

[0044] Alkaline protease: purchased from Shandong Qilu Biotechnology Group Co., Ltd.

[0045] Example 1: A composition of fox nuts, deer tendon collagen, and golden camellia 1. Preparation of an extract for improving sleep deprivation stress injury: Step 1: Dry the fox nuts and then pulverize them into a 100-mesh powder for later use; Step 2: Add 10 times the weight of the deer tendon raw material to purified water and a 3% citric acid aqueous solution, soak for 24 h, reflux extract at 95℃ for 1 h, filter, and collect the supernatant. Repeat the above process for the residue, filter, and collect the supernatant again. Combine the two supernatants and dialyze (molecular weight cutoff 50 kDa, 20℃, 120 h) until the pH of the dialysate (i.e., protein solution) is 7. Collect the supernatant; add 1000 U / g alkaline protease to the supernatant and enzymatically hydrolyze at 40℃ for 24 h. Then, heat the reaction system to 100℃ and hold for 10 min to inactivate the enzyme; after centrifugation, collect the supernatant, freeze-dry, and obtain deer tendon collagen powder.

[0046] Step 3: After drying and pulverizing the Camellia chrysantha, add 10 times the weight of the herb in purified water and reflux at 95°C for 1.5 h. Filter through a 100 μm membrane and collect the supernatant. Repeat the above process on the residue and collect the supernatant again. Combine the two supernatants, concentrate under reduced pressure, concentrate 10 times, collect the supernatant, and freeze-dry to obtain Camellia chrysantha extract.

[0047] Step 4: Mix 60% of the Euryale ferox powder from Step 1, 25% of the deer tendon collagen powder from Step 2, and 15% of the Camellia chrysantha extract from Step 3 to form a compound extract by weight percentage.

[0048] 2. Establishment of a mouse sleep deprivation model The experiment was conducted one week after C57BL / 6J mice (22-25 g) had acclimatized to the experimental environment. Ten mice were randomly assigned to each group.

[0049] Husbandry conditions: The animal laboratory facility maintains a consistently high barrier environment standard. Key environmental parameters are controlled within the following ranges: Temperature 23.1±0.9℃; Relative humidity 52.8±3.0%; Air exchange rate 10-20 times / hour; Light:Dark ratio 12 h:12 h. Husbandry and management are conducted by qualified personnel. Bedding and cages are changed and disinfected twice weekly to maintain a clean and dry environment; feed and water are provided daily, allowing animals free access to eat and move around.

[0050] Dosage regimen: The drug was administered once daily by gavage for 4 weeks, followed by sleep deprivation: Mice were deprived of sleep for 48 hours continuously, and were orally administered once daily during the sleep deprivation period.

[0051] Dosage: 200 mg / 10 g mouse body weight.

[0052] Mice were placed in an automated sleep deprivation system and acclimatized for 4 hours. A stainless steel rod was pre-fixed in the center of each plastic cage (11 cm high, 30 cm in diameter). Sleep was then deprived for 48 hours through timed, randomly rotating motions to ensure the mice remained awake and unable to fall asleep. Mice from the same cage (n = 5) were placed in a sleep deprivation device with free access to food and water. Behavioral tests were then performed on each group of mice, and finally, they were dissected to collect blood and tissue samples.

[0053] 3. Mouse behavioral tests 3.1 Diving Platform Experiment The platform test is a common method for neurobehavioral assessment of mice after drug and other interventions. The experimental setup consists of a dark box with an electrically conductive fence at the bottom and an insulated platform in the center.

[0054] Training phase: Mice were placed in a dark chamber beforehand and allowed to explore freely. After acclimatizing for 3 minutes, they were immediately stimulated with 32 V AC current for 5 seconds. Then, the mice were placed on an insulated platform for a period of time to acclimatize. During the training phase, it was ensured that the mice learned to stay on the platform to avoid electric shock.

[0055] Testing Phase: Testing was conducted 24 hours after the end of training. The mice were placed back on the insulated platform, and timing began. Records were kept for each mouse. (1) Latency period: The time (s) from when the mouse is placed on the platform in the dark box until it jumps off the platform for the first time. (2) Number of errors: The number of times the mouse jumped off the platform within the 5-minute test time.

[0056] 3.2 Water Maze Test The Morris water maze is a circular pool, 120 cm in diameter and 50 cm high, with a water temperature of 25 ± 1 ℃. The pool is divided into four quadrants centered on its center: Northeast, Northwest, Southeast, and Southwest (NE, NW, SW, SE). A suitable amount of milk powder was added to the pool to color the surface milky white, and a platform was placed 1 cm underwater. Mice were gently placed into the water from the center of any quadrant. The mice's swimming behavior was captured by a camera fixed directly above the maze, simultaneously capturing and analyzing their movement trajectories and recording the latency of finding the platform. The video was analyzed using Ethvision 11.5. Each mouse completed the experiment twice daily for five consecutive days. Latency (s) was defined as the time (s) it took for the mouse to find the hidden platform in the target quadrant. On day 5, after 48 hours of sleep deprivation, the mice's swimming speed (cm / s) and latency (s) were monitored.

[0057] 4. Mouse hippocampal index test Following behavioral testing, mice were immediately euthanized by cervical dislocation. The hippocampus was then dissected on ice and stored in PBS buffer (pH 7.4). Acetylcholine (ACh) and reactive oxygen species (ROS) levels in the hippocampus were measured according to the ELISA kit instructions.

[0058] Comparative Example 1 The mice were subjected to animal experiments without any treatment.

[0059] Comparative Example 2 This comparative example uses the same amount of distilled water as the combined extract in Example 1 for animal experiments.

[0060] Comparative Example 3 This comparative example uses the same amount of caffeine as the combined extract in Example 1 for animal experiments.

[0061] The extracts obtained in Example 1 and Comparative Examples 1-3 were subjected to performance tests, and the test results are shown in Table 1: Table 1 Test results of Example 1 and Comparative Examples 1-3

[0062] As shown in Table 1, compared with the blank group (Comparative Example 1), the model group (Comparative Example 2) mice showed a significantly reduced latency in the platform jumping test and a significantly increased number of errors; in the water maze test, the latency of mice finding the platform was prolonged, and their swimming speed was significantly reduced; the acetylcholine content in the hippocampus was reduced, and reactive oxygen species were excessively produced. The combination of Euryale ferox, deer tendon collagen, and Camellia chrysantha (Example 1) showed a more significant improvement effect compared with the caffeine group (Comparative Example 3), namely: significantly increasing the latency of sleep-deprived mice in the platform jumping test and reducing the number of errors; reducing the latency of mice in the water maze test and increasing their swimming speed; increasing the acetylcholine content in the hippocampus and clearing ROS accumulation.

[0063] Example 2 This embodiment provides a composition for improving sleep deprivation stress injury, comprising 70% of the composition of Example 1, 30% tilapia collagen, and the rest being consistent with Example 1, to obtain the composition.

[0064] Example 3 This embodiment provides a composition for improving sleep deprivation stress injury, comprising 70% of the composition of Example 1, 20% red ginseng extract, and 10% blueberries, with the other components remaining the same as in Example 1. The blueberries are lyophilized powder.

[0065] Preparation method of blueberry freeze-dried powder: Wash fresh, unrotten blueberries and drain them; crush and homogenize the washed blueberries at 4℃ to obtain blueberry puree; pasteurize the blueberry puree (80℃ for 15 min); pre-freeze the pasteurized blueberry puree at -80℃ for 4 h, then transfer it to a freeze dryer and freeze-dry at -50℃ for 24 h to obtain blueberry freeze-dried powder (moisture content <3%).

[0066] Example 4 This embodiment provides a composition for improving sleep deprivation stress injury, comprising 70% of the composition of Example 1, 30% caffeine, and other components consistent with Example 1, to obtain the composition.

[0067] Example 5 This embodiment provides a composition for improving sleep deprivation stress injury, comprising 70% of the composition of Example 1, 30% taurine, and the rest being consistent with Example 1, to obtain the composition.

[0068] Example 6 This embodiment provides a composition for improving sleep deprivation stress injury, comprising 70% of the composition of Example 1, 30% of B vitamins (20% vitamin B1, 15% vitamin B2, 40% vitamin B3, 15% vitamin B6, and 10% vitamin B1), with the other components remaining the same as in Example 1, to obtain the composition.

[0069] Comparative Example 4 The specific implementation method is the same as in Example 2, except that it uses 100% tilapia collagen.

[0070] Comparative Example 5 The specific implementation method is the same as in Example 2, except that it contains 70% caffeine and 30% tilapia collagen.

[0071] Table 2 Test results of Examples 2-6 and Comparative Examples 4-5

[0072] As can be seen from Table 2, compared with the composition of Example 1 alone, the combination of the composition with active substances such as tilapia collagen, red ginseng, blueberry, caffeine, taurine and B vitamins can exert a significant synergistic effect, greatly increasing the latency of mice in the jumping platform test and reducing the number of errors; reducing the latency of mice in the water maze test and increasing swimming speed; increasing the Ach content in the hippocampus and clearing ROS.

[0073] Compared to tilapia collagen (Comparative Example 4) and caffeine combined with tilapia collagen (Comparative Example 5), the combination of foxnut, deer tendon collagen and golden camellia, along with active substances such as tilapia collagen, red ginseng, blueberry, caffeine, taurine and B vitamins, can exert a greater effect in improving sleep deprivation stress damage, that is, improving the cognitive function and learning and memory ability of sleep-deprived mice.

[0074] Comparative Example 6 The specific implementation method is the same as in Example 1, except that (1) Euryale ferox is omitted; (2) deer tendon collagen is omitted; and (3) golden camellia extract is omitted.

[0075] Table 3 Test results of Comparative Examples 6 (1–3)

[0076] The results showed that omitting any component affected the cognitive function and learning and memory abilities of sleep-deprived mice.

[0077] Comparative Example 7 The specific implementation method is the same as in Example 1, except that the foxnut is replaced with yam.

[0078] The results showed that replacing foxnut with yam affected the effect of the composition on the cognitive function and learning and memory abilities of sleep-deprived mice.

[0079] Comparative Example 8 The specific implementation method is the same as in Example 1, except that deer tendon collagen is replaced with pig skin collagen.

[0080] The results showed that replacing deer tendon collagen with pig skin collagen affected the effect of the composition on cognitive function and learning and memory abilities in sleep-deprived mice.

[0081] Comparative Example 9 The specific implementation method is the same as in Example 1, except that the golden camellia extract is replaced with oolong tea extract.

[0082] The results showed that replacing camellia extract with oolong tea extract affected the effect of the composition on cognitive function and learning and memory abilities in sleep-deprived mice.

[0083] Table 4 Test results of comparative examples 7–9

[0084] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A composition for improving sleep deprivation stress injury, characterized in that, By weight percentage: 50%~70% foxnut, 20%~30% collagen, 10%~20% golden camellia extract.

2. The composition according to claim 1, characterized in that, The gorgon fruit is pre-treated by pulverizing, and then pulverized to 50-200 mesh; The collagen is derived from deer tendons; The collagen is prepared by using deer tendon as raw material, and then undergoing acid extraction, dialysis, enzymatic hydrolysis, and freeze-drying. The preparation method of the golden camellia extract is as follows: using golden camellia as raw material, it is obtained by water extraction, filtration, concentration and freeze drying.

3. The composition according to claim 1 or 2, characterized in that, It comprises, by weight percentage: 50% to 80% of the composition of claim 1 or 2; and any of the following additional components: (1) 20%~50% tilapia collagen; or, (2) 10%~30% red ginseng, 5%~15% blueberry; or, (3) 20%~50% caffeine; or, (4) 20%~50% taurine; or, (5) 20%~50% B vitamins.

4. A drug, characterized in that, The composition comprises any one of claims 1 to 3.

5. A method for preparing the composition according to any one of claims 1 to 3, characterized in that, The method steps are as follows: (1) Dry the fox nuts and then pulverize them into powder; (2) Deer tendon collagen was prepared by acid extraction, dialysis, enzymatic hydrolysis and freeze drying. (3) A golden camellia extract was prepared by water extraction, filtration, concentration and freeze drying using golden camellia as raw material; (4) Mix 50% to 70% of the Euryale ferox powder from step (1), 20% to 30% of the deer tendon collagen powder from step (2), and 10% to 20% of the Camellia chrysantha extract from step (3) by mass percentage to form a compound extract.

6. The method according to claim 5, characterized in that, In step (1), the powder particle size is 50-200 mesh.

7. The method according to claim 5, characterized in that, In step (2), acid extraction involves adding 5 to 15 times the weight of the raw material of deer tendon to purified water and an acidic aqueous solution with a concentration of 3 to 5%, soaking, reflux extraction, filtration, repeated extraction of the residue, and combining the two extracts. In step (2), dialysis is performed using dialysis bags with a molecular weight cutoff of 5-50 kDa; In step (2), enzymatic hydrolysis is performed using protease; In step (3), add 5 to 15 times the weight of the raw material of purified water to the golden camellia, reflux extract for 1 to 1.5 h, filter with a 10 to 100 μm membrane, extract the residue repeatedly, and combine the two extracts; In step (3), the volume is concentrated to 10% to 30% of the original volume.

8. Use of the composition according to any one of claims 1 to 3 in the preparation of products for preventing and / or improving sleep deprivation stress injury.

9. The application according to claim 8, characterized in that, The product is a functional food, dietary supplement, or medicine.

10. The application according to claim 8 or 9, characterized in that, The sleep deprivation stress injury includes depression, bipolar disorder, anxiety, PTSD, schizophrenia, Alzheimer's disease, Parkinson's disease, traumatic brain injury, and cognitive impairment. Optionally, the cognitive impairment includes memory decline, inattention, or decreased learning ability.

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