Cosmetic composition containing polydeoxyribonucleotide (PDRN) for promoting skin regeneration

Abstract

The present invention relates to a cosmetic composition for promoting skin regeneration, and more particularly, to a cosmetic composition for promoting skin regeneration by further comprising a polydeoxyribonucleotide (PDRN) in a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio, and more particularly, to a cosmetic composition for promoting skin regeneration by adding a polydeoxyribonucleotide (PDRN) to a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio. The sponge microneedle forms a microchannel in a skin cuticle to promote transmission of effective components, the lactic acid bacteria exosome induces cell viability through a cell signal transduction function, and the ceramide strengthens a skin barrier. By adding PDRN in the range of 0.001 to 3% by weight relative to the total weight of the composition, proliferation of skin fibroblasts, collagen synthesis and wound healing can be promoted. The composition can be prepared into various dosage forms such as face cream, emulsion, essence, ampoules or facial masks, and the improved skin regeneration effect is provided by simultaneously achieving the effects of strengthening the skin barrier, activating cells and promoting tissue regeneration.

Description

Technical Field

[0001] This invention relates to a cosmetic composition comprising polydeoxyribonucleotide (PDRN) for promoting skin regeneration. Background Technology

[0002] As the outermost organ of the human body directly exposed to the external environment, the skin is subject to continuous damage from ultraviolet radiation, fine particulate matter, oxidative stress, and physical irritants. In particular, with aging, the proliferative and regenerative abilities of skin cells decline, leading to reduced collagen synthesis, increased inflammation, and delayed wound healing. These changes result in wrinkles, decreased elasticity, and a weakened skin barrier function.

[0003] Accordingly, various functional cosmetic compositions aimed at promoting skin regeneration are currently under continuous development. For example, technologies have been developed that utilize ceramides to strengthen the skin barrier, utilize exosomes to activate cell signaling, and utilize microneedle-like structures to promote the delivery of active ingredients. However, existing technologies primarily focus on improving the skin barrier or activating cells, with relatively limited formulations that directly promote the recovery and regeneration of damaged tissue.

[0004] On the other hand, polydeoxyribonucleotides (PDRNs) are substances composed of DNA fragments of a certain length and have been reported to possess physiological activities related to tissue regeneration and anti-inflammatory effects. In particular, PDRNs are known to promote cell proliferation and angiogenesis, alleviate inflammatory responses, and promote the recovery of damaged tissues by mediating the adenosine A2A receptor pathway. Due to these properties, PDRNs have been applied in the fields of medicine and regenerative therapy. However, when PDRNs are used in cosmetic compositions, the delivery of their active ingredients may be limited by the skin surface barrier, and the regenerative effect may not be fully realized when used alone. Furthermore, a complex pathway of cell activation and barrier stabilization is required to promote skin regeneration. Therefore, there is a need to develop a composition that includes structures that promote the delivery of active ingredients, components that provide cell signaling functions, and components that strengthen the skin barrier, while also directly promoting tissue regeneration. Summary of the Invention

[0005] This invention aims to overcome the limitations of existing cosmetic compositions that restrict the delivery of active ingredients and hinder tissue regeneration due to the skin surface barrier. Specifically, existing skin-improving compositions focus on strengthening the skin barrier or activating cells, but may lack the ability to directly promote the recovery and regeneration of damaged tissue. In particular, decreased proliferation of skin fibroblasts and reduced collagen synthesis lead to decreased skin elasticity and delayed wound healing, thus requiring regeneration-promoting technologies to compensate for these shortcomings.

[0006] Accordingly, the objective of this invention is to provide a cosmetic composition for promoting skin regeneration by including polydeoxyribonucleotides (PDRN) in a specific weight ratio of a composition comprising sponge microneedles, lactic acid bacteria exosomes, and ceramides, thereby ensuring efficient delivery to the skin surface while directly promoting tissue regeneration. Furthermore, another objective of this invention is to provide a composition that promotes the proliferation of dermal fibroblasts and accelerates wound healing.

[0007] According to an embodiment of the present invention, a cosmetic composition for promoting skin regeneration comprises a sponge microneedle, lactic acid bacteria exosomes, and ceramides in a weight ratio of 0.6:3.0:0.01. The lactic acid bacteria exosomes are obtained by concentrating Lactobacillus curvatus GFC-AM5 strain (accession number: KCCM12245P) at 37°C for 20 hours at a concentration of more than 40 times, and contain 0.001 to 3% by weight of polydeoxyribonucleotides (PDRN) relative to the total weight.

[0008] The average molecular weight of the polydeoxyribonucleotide is in the range of 50 kDa to 1500 kDa. The cosmetic composition is characterized by promoting the proliferation of skin fibroblasts and promoting wound healing. Furthermore, the cosmetic product according to the invention can be a cream, lotion, serum, ampoule, or sheet mask containing a cosmetic composition for promoting skin regeneration.

[0009] The cosmetic composition for promoting skin regeneration according to the present invention has the following effects:

[0010] First, by activating the adenosine receptor pathway through PDRN, it can promote skin cell proliferation and tissue regeneration.

[0011] Secondly, through the microchannels formed by the sponge microneedles, PDRN and lactic acid bacteria exosomes can be efficiently delivered into the skin and interact with the cell signal transduction function of the exosomes, thereby enhancing the regeneration effect.

[0012] Third, ceramides stabilize the skin barrier, protect the skin from external stimuli, and keep the regeneration process stable.

[0013] Fourth, the composition according to the present invention can promote the proliferation of skin fibroblasts and improve the speed of wound healing, thereby helping to improve skin elasticity and restore damage. Therefore, the present invention provides a further improved skin regeneration effect compared to existing compositions by simultaneously strengthening the skin barrier, activating cell signaling, and promoting tissue regeneration. Attached Figure Description

[0014] Figure 1 is a diagram that briefly illustrates the mechanism of action of a cosmetic composition for promoting skin regeneration containing polydeoxyribonucleotides (PDRN) according to an embodiment of the present invention.

[0015] Figure 2 is a graph showing the results of treating skin fibroblasts with compositions containing different PDRN contents and comparing cell proliferation indicators.

[0016] Figure 3 is a graph showing the results of cell proliferation indices measured after treatment of the skin fibroblasts with the compositions of Example 1 and Comparative Example 1.

[0017] Figure 4 is a graph showing the results of treatment in Example 1 and Comparative Example 1 after scratch formation by skin fibroblasts, and comparing the wound closure rate.

[0018] Figure 5 is a graph showing the results of measuring collagen expression levels after skin fibroblast treatment with the compositions of Example 1 and Comparative Example 1. Detailed Implementation

[0019] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can readily implement the invention. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. For clarity of illustration, parts irrelevant to the description have been omitted from the drawings, and throughout the specification, the same reference numerals are used for the same or similar constituent elements. In this specification, terms such as "comprising" or "having" are intended to specify the presence of features, numbers, steps, actions, constituent elements, parts, or combinations thereof, without presuming the possibility of the presence or addition of other features or combinations. Furthermore, hierarchical descriptions regarding "above" or "below" include both "directly" and cases where other parts are present in between.

[0020] Referring to Figure 1, the skin consists of the stratum corneum, epidermis, and dermis. Microchannels can be formed in the stratum corneum using sponge microneedles applied to the skin surface. Through these microchannels, lactic acid bacteria exosomes, ceramides, and PDRN can be delivered into the skin. The delivered PDRN promotes fibroblast proliferation and increases collagen synthesis in the dermis, thereby promoting tissue regeneration. It also works in conjunction with the cell signaling function of exosomes to enhance skin regeneration.

[0021] The cosmetic composition for promoting skin regeneration according to the present invention comprises sponge microneedles, lactic acid bacteria exosomes, ceramides, and polydeoxyribonucleotides (PDRN). Preferably, the sponge microneedles, lactic acid bacteria exosomes, and ceramides are contained in a weight ratio of 0.6:3.0:0.01. This weight ratio is based on a combination ratio proven to have excellent skin barrier stabilizing and cell activation effects. The lactic acid bacteria exosomes are obtained by culturing Lactobacillus curvatus GFC-AM5 strain (accession number: KCCM12245P) at 37°C for 20 hours and then concentrating it more than 40 times. The sponge microneedles may be of hydrolyzed sponge origin. The ceramide may be yeast-derived ceramide NP, which provides functions of strengthening the skin barrier and maintaining moisture.

[0022] In this invention, the PDRN content is preferably 0.001 to 3% by weight relative to the total weight of the cosmetic composition, more preferably 0.01 to 1% by weight. Below this lower limit, the effect of promoting skin fibroblast proliferation may be limited; above this upper limit, the increase in effect tends to saturate, and the additional regeneration promotion effect is negligible. Referring to Figure 2, in the range where the NMN content is below 0.001% by weight, the increase in cell proliferation indicators is limited, while in the range of 0.001 to 3% by weight, the cell proliferation indicators show a significant increasing trend. Furthermore, in the range exceeding 3% by weight, the increase in proliferation indicators tends to saturate, which supports the critical significance of the PDRN content range proposed in this invention. The 0.01 to 1% by weight range exhibits stable and excellent effects and can be set as a more preferred range. The average molecular weight of the PDRN is preferably in the range of 50 kDa to 1500 kDa and can be extracted from salmon testes. Furthermore, PDRN can promote cell proliferation and tissue regeneration by activating the adenosine A2A receptor pathway.

[0023] In the preparation method, a basic composition is prepared by uniformly mixing sponge microneedles, lactic acid bacteria exosomes, and ceramides according to the stated weight ratio. Subsequently, PDRN is added to the composition within a predetermined content range and stirred uniformly to prepare the cosmetic composition according to the present invention. During the preparation process, PDRN can be added in an aqueous solution, and the pH of the composition is preferably adjusted to the range of 5.0 to 6.5. This range is intended to maintain conditions similar to the natural slightly acidic environment of the skin, thereby minimizing skin irritation while ensuring the structural stability of PDRN. Generally, if the pH is too low (below 5.0), the possibility of partial denaturation or hydrolysis of the nucleic acid structure increases, leading to a decrease in the physiological activity of PDRN; conversely, if the pH exceeds 6.5, stability may decrease or interactions with exosomes and ceramides may change during long-term storage. Therefore, the present invention sets the pH within the range of 5.0 to 6.5.

[0024] Example 1: Preparation of a composition containing PDRN. A basic composition was prepared by mixing sponge microneedles, lactic acid bacteria exosomes, and ceramides in a weight ratio of 0.6:3.0:0.01. The lactic acid bacteria exosomes were obtained by concentrating Lactobacillus curvatus GFC-AM5 strain more than 40 times after culturing at 37°C for 20 hours. Subsequently, PDRN with an average molecular weight of approximately 100 kDa was added to a content of 0.1% by weight relative to the total composition, and the mixture was stirred uniformly to prepare the composition of the present invention.

[0025] Comparative Example 1: A composition without PDRN was prepared as a base composition in the same manner as in Example 1, but without the addition of PDRN, and used as a comparative group.

[0026] Experimental Example 1: Evaluation of Fibroblast Proliferation Effect. Cell proliferation rate was measured after treating skin fibroblasts with the compositions of Example 1 and Comparative Example 1. The results showed that the treatment group containing PDRN (Example 1) exhibited an increased cell proliferation rate compared to Comparative Example 1. Referring to Figure 3, Comparative Example 1 without PDRN showed a certain level of increased proliferation compared to the control group, while Example 1 containing PDRN showed a higher cell proliferation index. Therefore, PDRN can enhance tissue regeneration by promoting fibroblast proliferation.

[0027] Experimental Example 2: Evaluation of Wound Healing Effect. After scratches were formed on dermal fibroblasts, Examples 1 and Comparative Example 1 were treated, and the wound closure rate was measured. The results showed that the Example 1 treatment group, containing PDRN, exhibited a higher wound recovery rate than Comparative Example 1 within the same timeframe. Referring to Figure 4, the wound closure rate of the Example 1 treatment group showed a significant increasing trend. Therefore, the composition of the present invention can enhance skin regeneration by promoting wound healing.

[0028] Experimental Example 3: Effect of Increased Collagen Synthesis Collagen expression levels were analyzed after treating skin fibroblasts with the compositions of Example 1 and Comparative Example 1. The results showed that the treatment group of Example 1, containing PDRN, exhibited an increased trend in collagen synthesis levels compared to Comparative Example 1. Therefore, it can be concluded that the compositions of the present invention can help improve elasticity and tissue regeneration by promoting collagen synthesis in the dermis of the skin.

[0029] The compositions according to the present invention can be prepared into various cosmetic formulations such as creams, lotions, serums, ampoules, sheet masks, or gels. The sponge microneedles contained in the formulation form microchannels when applied to the skin, promoting the delivery of PDRN and exosomes. PDRN directly promotes tissue regeneration, exhibiting wound healing and improved elasticity effects.

[0030] The embodiments of the present invention have been described above, but the concept of the present invention is not limited thereto. Those skilled in the art can make additions, changes, or deletions within the same conceptual framework, and these should also fall within the scope of the present invention.

Claims

1. A cosmetic composition for promoting skin regeneration, characterized in that, include: The composition comprises sponge microneedles, lactic acid bacteria exosomes, and ceramide, wherein the sponge microneedles, lactic acid bacteria exosomes, and ceramide are contained in a weight ratio of 0.6:3.0:0.01; the lactic acid bacteria exosomes are obtained by culturing Lactobacillus curvatus GFC-AM5 strain (accession number: KCCM12245P) at 37°C for 20 hours and then concentrating it more than 40 times; and contains 0.001 to 3% by weight of polydeoxyribonucleotides (PDRN) relative to the total weight of the composition.

2. The cosmetic composition for promoting skin regeneration as described in claim 1, characterized in that, The average molecular weight of the polydeoxyribonucleotide is in the range of 50 kDa to 1500 kDa.

3. The cosmetic composition for promoting skin regeneration as described in claim 1 or 2, characterized in that, The polydeoxyribonucleotides were extracted from salmon testes.

4. The cosmetic composition for promoting skin regeneration as described in any one of claims 1 to 3, characterized in that, The cosmetic composition promotes the proliferation of skin fibroblasts and promotes wound healing.

5. A cosmetic product, characterized in that, A cosmetic composition comprising any one of claims 1 to 4 for promoting skin regeneration, wherein the dosage form is a cream, lotion, serum, ampoule or sheet mask.

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