Composite essential oil with anti-wrinkle effect as well as preparation method and application of composite essential oil

Abstract

The invention provides compound essential oil with an anti-wrinkle effect and a preparation method and application of the compound essential oil, and belongs to the technical field of skin care product preparation. 5%-20% of frankincense essential oil; 5%-20% of sandalwood essential oil; 5%-15% of litsea cubeba essential oil; 10%-20% of myrrh essential oil; the compound essential oil is prepared through the processes of step-by-step constant-temperature stirring, nitrogen protection, dark curing and filtering and can be applied to skin care products such as essence oil, face cream, emulsion, eye cream or massage oil, and the adding amount is 1%-3%. The anti-wrinkle and skin-brightening essential oil achieves the effects of synergistically resisting oxidation, promoting collagen metabolism and repairing skin barrier of all the components, the anti-wrinkle and skin-brightening comprehensive effect is prominent, the preparation process is matched with the characteristics of active components of the essential oil, component loss is effectively reduced, the product stability is improved, the components are natural and mild, and the anti-wrinkle and skin-brightening essential oil conforms to relevant provisions of Cosmetic Safety Technical Specifications, is high in safety, and is suitable for industrial production. The mask is suitable for long-term use of various skins.

Description

Technical Field

[0001] This invention relates to the field of skin care product preparation technology, and in particular to a compound essential oil with anti-wrinkle effects, its preparation method, and its application. Background Technology

[0002] Skin aging is a complex process resulting from both internal physiological decline and external environmental damage, manifesting as wrinkles, sagging, and decreased barrier function. In recent years, skincare strategies based on natural plant active ingredients have gained significant popularity. Among these, essential oils, rich in small-molecule active substances such as terpenes and esters, are easily absorbed through the skin and exhibit multiple biological activities including anti-inflammation and repair, making them promising candidates for anti-wrinkle treatment. For example, frankincense and sandalwood essential oils have been reported in the literature to promote skin cell renewal and alleviate photoaging. However, the targets and efficacy dimensions of a single essential oil are relatively limited, making it difficult to comprehensively address the multi-pathway mechanisms of skin aging.

[0003] To enhance efficacy, existing technologies primarily employ two approaches: First, a simple physical mixture of multiple single essential oils claiming anti-wrinkle effects. While convenient, this method overlooks the compatibility and synergy between the different chemical components of these essential oils. The active ingredients in essential oils (such as monoterpenes and sesquiterpenes) are chemically reactive. Mixing them without optimized formulation and stabilization processes can easily lead to oxidation, polymerization, or the release of volatile components, resulting in uncontrollable levels of active ingredients, unstable efficacy, and potentially even the formation of irritants. Second, high concentrations of synthetic anti-wrinkle active ingredients (such as retinoids and peptides) are directly added to the formula. While these ingredients have clear mechanisms and rapid onset of action, they generally suffer from strong skin irritation and poor stability (such as photosensitivity), making them unsuitable for long-term use on sensitive skin and falling short of market expectations for gentle, highly effective, and natural skincare products.

[0004] Therefore, there is an urgent need for a compound essential oil that combines significant anti-wrinkle effects with good stability to solve the aforementioned problems. Summary of the Invention

[0005] To overcome the shortcomings of the prior art, the purpose of this invention is to provide a compound essential oil with anti-wrinkle effects, its preparation method and application. By compounding five essential oils in the disclosed proportions and optimizing the preparation process, the compound essential oil achieves a synergistic anti-wrinkle effect while taking into account product stability and gentleness, making it suitable for applications in skin care products and other fields.

[0006] To achieve the above objectives, the present invention provides the following solution: On one hand, the present invention provides a compound essential oil with anti-wrinkle effects, wherein the raw materials of the compound essential oil, by volume percentage, include: Clove essential oil 20%-50%; Frankincense essential oil 5%-20%; Sandalwood essential oil 5%-20%; Litsea cubeba essential oil 5%-15%; Myrrh essential oil 10%-20%.

[0007] Preferably, the raw materials of the compound essential oil, by volume percentage, include: Clove essential oil 45%; Frankincense essential oil 15%; Sandalwood essential oil 15%; 10% Litsea cubeba essential oil; Myrrh essential oil 15%.

[0008] Secondly, the present invention provides a method for preparing the above-mentioned compound essential oil with anti-wrinkle effects, comprising the following steps: S1. Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir at 15-25℃ until a homogeneous solution is obtained to obtain the first mixed phase; S2. Add pheasant oil to the first mixed phase and stir at 20-30℃ until a homogeneous solution is obtained to obtain the second mixed phase; S3. Add frankincense essential oil to the second mixed phase and mix evenly at 25-35℃ with stirring to obtain the third mixed phase; S4. Add clove essential oil to the third mixed phase, controlling the addition time to 5-15 minutes. After mixing, seal and let it stand in the dark at 25-30℃ for 24-48 hours to mature. After filtration, the compound essential oil is obtained.

[0009] Preferably, in steps S1 and S2, the raw materials are mixed under nitrogen protection.

[0010] Preferably, in step S3, the stirring conditions are stirring at a rate of 300-500 r / min for 10-15 minutes.

[0011] Preferably, in step S4, the curing time is 36 hours.

[0012] Preferably, in step S4, the filtration is performed using a filter membrane or filter paper with a pore size of 0.22μm-10μm.

[0013] On the other hand, the present invention also provides an application of the above-mentioned compound essential oil with anti-wrinkle effect in skin care products.

[0014] Preferably, the amount of the compound essential oil added to the skin care product is 1%-3%.

[0015] Preferably, the skin care product is one of the following: essential oil, face cream, lotion, eye cream, or massage oil.

[0016] Compared with the prior art, the present invention discloses at least the following technical effects: (1) This invention solves the problems of limited efficacy of single essential oils and poor results from arbitrary combinations. The antioxidant effect of clove essential oil, the collagen metabolism-promoting effect of frankincense essential oil, the skin barrier repair ability of sandalwood essential oil, the free radical scavenging effect of litsea cubeba essential oil, and the dermal repair advantage of myrrh essential oil complement each other. By combining them in a limited volume percentage, synergistic effects are achieved. This invention can improve skin aging problems such as collagen loss, elastic fiber breakage, and free radical damage from multiple aspects, avoiding the antagonistic effects of different essential oil combinations. Compared with single essential oils or products that are randomly combined, the anti-wrinkle and brightening effects are more comprehensive and significant.

[0017] (2) The present invention, through the disclosed preparation method, reduces the loss of active ingredients in essential oils, improves product stability, and solves the problems of easy oxidation and loss of volatile components in existing simple mixing of essential oils. A step-by-step constant-temperature stirring method is adopted to adapt to the viscosity and solubility characteristics of different essential oils; nitrogen protection reduces the oxidation loss of active ingredients; and light-proof static maturation allows the active substances of each essential oil to fully integrate. Reasonable control of stirring speed and maturation time ensures a uniform and stable mixing system. Compared with simple mixing and lack of stabilization measures, the present invention can effectively extend the product's shelf life and ensure a stable and lasting anti-wrinkle effect.

[0018] (3) This invention takes into account the natural gentleness and applicability of the product, solving the problem that existing anti-wrinkle products cannot simultaneously achieve effective anti-wrinkle and gentle skin care. The formula uses all natural plant essential oils and does not add any synthetic irritating ingredients. It has been verified by human skin patch tests to be highly safe and meets the relevant requirements of the "Cosmetic Safety Technical Specifications". It is suitable for long-term use of all types of skin. At the same time, it can be flexibly added to a variety of skin care products. The addition ratio of 1%-3% can achieve obvious effects. Compared with synthetic anti-wrinkle products with strong irritation, it better meets consumers' needs for natural and gentle skin care and has a wider range of applicable scenarios. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 A flowchart illustrating the preparation method of the anti-wrinkle compound essential oil provided by this invention; Figure 2 This is a bar chart comparing the number of wrinkles after use in the embodiments and comparative examples of the present invention; Figure 3This is a bar chart comparing the wrinkle depth after use in the embodiments and comparative examples of the present invention; Figure 4 This is a bar chart comparing the wrinkle area after use in the embodiments and comparative examples of the present invention; Figure 5 This is a bar chart comparing the wrinkle length after use in the embodiments and comparative examples of the present invention. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] I. Main Instruments and Materials Clove (SYZYGIUMAROMATICUM) essential oil: The main components are eugenol, acetyleugenol, and caryophyllene. Clove essential oil has antioxidant properties and can inhibit free radical damage. It can soothe the skin's aging stress response, help improve fine lines, and enhance the stability of the skin's surface barrier.

[0024] Frankincense (BOSWELLIA CARTERII) essential oil: Its main components are α-pinene, limonene, p-cymene, myrcene, and sapinene. Frankincense essential oil can promote skin microcirculation, aid in skin collagen metabolism and repair of damaged tissue, diminish dynamic fine lines and static wrinkles, and also has a soothing effect on the skin, improving roughness. It is suitable for anti-wrinkle care for mature skin.

[0025] Sandalwood essential oil: Its main components are α and β-santalol, santalene, santalol, and bisabolol. Sandalwood essential oil is a gentle, skin-friendly astringent that can regulate the skin's oil-water balance, firm loose skin, reduce the appearance of fine lines, repair the skin barrier, and delay the deepening of wrinkles caused by dryness and oxidation, thus meeting the anti-wrinkle needs of both dry and mature skin.

[0026] Litsea cubeba essential oil: Its main components are citral, linalool, limonene, and α-pinene. Litsea cubeba essential oil possesses core functions of scavenging free radicals and providing antioxidant protection. It can reduce oxidative damage to the skin caused by external environmental factors, inhibit the degradation of proteins related to skin aging, improve dull and sagging skin, synergistically reduce wrinkles, and enhance skin elasticity.

[0027] Myrrh (COMMIPHORAMYRRHA) essential oil: Its main components are furanoic acid esterene, turmericene, camphorene, β-elemene, linderne, and geranylene. Myrrh essential oil can improve blood circulation, promote skin metabolism, repair the damaged dermal structure, improve pigmentation and wrinkles associated with mature skin, enhance skin firmness, and reduce the appearance of deep wrinkles.

[0028] It should be noted that all raw materials and testing equipment used in this application were purchased externally and meet the quality standards for cosmetic essential oils. Specifically, by volume percentage, the raw materials of the compound essential oil include: clove essential oil 20%-50%; frankincense essential oil 5%-20%; sandalwood essential oil 5%-20%; litsea cubeba essential oil 5%-15%; and myrrh essential oil 10%-20%.

[0029] Based on the above raw material composition, such as Figure 1 As shown, this invention provides a method for preparing a compound essential oil with anti-wrinkle effects, comprising the following steps: S1. Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir at 15-25℃ until a homogeneous solution is obtained to obtain the first mixed phase.

[0030] Specifically, the stirring rate is controlled at 300-500 r / min, and the stirring time is 8-12 min. A constant temperature stirring device is used to maintain the temperature at 20℃. During the stirring process, the mixture is observed until there is no layering, no visible particles, and the system is uniform and transparent. Stirring is then stopped to obtain the first mixed phase. This temperature range can prevent the active ingredients of the two essential oils from being degraded due to high temperature. The appropriate stirring rate and time can ensure that the two essential oils with slightly higher viscosity are fully integrated, laying the foundation for the subsequent mixing of components.

[0031] S2. Add pheasant oil to the first mixed phase and stir at 20-30℃ until a homogeneous solution is obtained to obtain the second mixed phase.

[0032] Specifically, adjust the temperature of the constant temperature stirring device to 25℃, maintain the stirring speed at 350-450r / min, slowly add the essential oil of Litsea cubeba (addition time controlled at 3-5min), and continue stirring for 5-8min until the mixture is uniform in color and there is no layering, thus obtaining the second mixed phase. The essential oil of Litsea cubeba is slightly volatile, so adding it slowly in steps and controlling the stirring speed can prevent its volatilization and loss, while ensuring that it is fully integrated with the first mixed phase to exert a synergistic antioxidant effect.

[0033] S3. Add frankincense essential oil to the second mixed phase and mix evenly at 25-35℃ with stirring to obtain the third mixed phase.

[0034] Specifically, the temperature of the constant temperature stirring device is raised to 30°C, and the stirring rate is maintained at 300-500 r / min. Frankincense essential oil is added all at once and stirring is continued for 10-15 minutes. The system status is observed every 3 minutes during this period until the mixture is homogeneous and viscous, with no obvious odor and stratification, thus obtaining the third mixed phase. Raising the temperature to this range can improve the solubility of frankincense essential oil, which is suitable for the dispersion requirements of its active ingredients. Sufficient stirring time can ensure that frankincense essential oil is fully combined with the previous mixed phase, thereby enhancing the synergistic effect of anti-wrinkle repair.

[0035] S4. Add clove essential oil to the third mixed phase, controlling the addition time to 5-15 minutes. After mixing, seal and let it stand in the dark at 25-30℃ for 24-48 hours to mature. After filtration, the compound essential oil is obtained.

[0036] Specifically, maintain a stirring speed of 400-500 r / min and add clove essential oil dropwise, strictly controlling the dropping rate to ensure completion within 10 minutes. After addition, continue stirring for 5 minutes to ensure complete homogeneity. Then, transfer the mixture to a brown sealed container and place it in a constant temperature and light-proof environment at 28℃ for 36 hours to mature. After maturation, use a 0.22μm filter membrane for vacuum filtration to remove trace amounts of insoluble impurities, resulting in a clear and transparent compound essential oil. The dropwise addition method avoids excessively high local concentrations of clove essential oil, light-proof maturation prevents oxidation of active ingredients, and the constant temperature environment promotes full reaction and fusion of active ingredients, enhancing the stability and longevity of the compound essential oil. The filter membrane size can be adjusted within the range of 0.22μm-10μm according to actual needs.

[0037] II. Conducting the Experiment Example 1 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 45% clove essential oil, 15% frankincense essential oil, 15% sandalwood essential oil, 10% litsea cubeba essential oil, and 15% myrrh essential oil.

[0038] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 20°C under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 25°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 12 minutes at 30°C and 400 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 10 minutes. After mixing evenly, seal the mixture and let it stand at 28°C in the dark for 36 hours to mature. Filter the mixture using a 0.22μm filter membrane to obtain the composite essential oil.

[0039] Example 2 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 25% clove essential oil, 20% frankincense essential oil, 20% sandalwood essential oil, 15% litsea cubeba essential oil, and 20% myrrh essential oil.

[0040] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 15°C under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 20°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 10 minutes at 25°C and 300 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 5 minutes. After mixing evenly, seal the mixture and let it stand at 25°C in the dark for 24 hours to mature. Filter the mixture using 10μm filter paper to obtain the composite essential oil.

[0041] Example 3 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 35% clove essential oil, 20% frankincense essential oil, 20% sandalwood essential oil, 15% litsea cubeba essential oil, and 10% myrrh essential oil.

[0042] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 25°C under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 30°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 15 minutes at 35°C and 500 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 15 minutes. After mixing evenly, seal the mixture and let it stand at 30°C in the dark for 48 hours to mature. Filter the mixture through a 1μm filter membrane to obtain the composite essential oil.

[0043] Example 4 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 44% clove essential oil, 12% frankincense essential oil, 13% sandalwood essential oil, 11% litsea cubeba essential oil, and 20% myrrh essential oil.

[0044] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 22°C under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 28°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 13 minutes at 32°C and 450 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 12 minutes. After mixing evenly, seal the mixture and let it stand at 27°C in the dark for 30 hours to mature. Filter the mixture through a 5μm filter membrane to obtain the composite essential oil.

[0045] Example 5 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 38% clove essential oil, 18% frankincense essential oil, 17% sandalwood essential oil, 12% litsea cubeba essential oil, and 15% myrrh essential oil.

[0046] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 18°C ​​under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 22°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 11 minutes at 28°C and 380 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 8 minutes. After mixing evenly, seal the mixture and let it stand at 26°C in the dark for 36 hours to mature. Filter the mixture using a 0.45μm filter membrane to obtain the composite essential oil.

[0047] Example 6 This embodiment provides a compound essential oil with anti-wrinkle effects. By volume percentage, the raw materials are: 45% clove essential oil, 8% frankincense essential oil, 12% sandalwood essential oil, 15% litsea cubeba essential oil, and 20% myrrh essential oil.

[0048] The preparation method of the compound essential oil includes the following steps: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 24℃ under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Add pheasant oil to the first mixed phase and stir at 26°C under nitrogen protection until a homogeneous solution is obtained to obtain the second mixed phase; Step 3: Add frankincense essential oil to the second mixed phase and mix for 14 minutes at 33°C and 420 r / min to obtain the third mixed phase; Step 4: Slowly add clove essential oil to the third mixed phase, controlling the dripping time to 14 minutes. After mixing evenly, seal the mixture and let it stand at 29°C in the dark for 42 hours to mature. Filter the mixture through a 2μm filter membrane to obtain the composite essential oil.

[0049] Comparative Example 1 This comparative example provides a control compound essential oil, which, by volume percentage, consists of: 38% clove essential oil, 17% frankincense essential oil, 15% sandalwood essential oil, 30% myrrh essential oil, and completely lacks litsea cubeba essential oil.

[0050] The preparation method is completely consistent with that in Example 1: Step 1: Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir them at 20°C under nitrogen protection until a homogeneous solution is obtained to obtain the first mixed phase; Step 2: Skip the step of adding litsea cubeba essential oil and directly add frankincense essential oil to the first mixed phase. Mix for 12 minutes at 30℃ and 400r / min to obtain the second mixed phase. Step 3: Add clove essential oil dropwise to the second mixed phase over 10 minutes. After sealing, mature at 28°C in the dark for 36 hours. Filter through a 0.22μm filter membrane to obtain the control essential oil.

[0051] Comparative Example 2 This comparative example provides a control compound essential oil, with the following raw material ratio by volume percentage: clove essential oil 55%, frankincense essential oil 4%, sandalwood essential oil 22%, litsea cubeba essential oil 16%, and myrrh essential oil 3%.

[0052] The preparation method is completely consistent with that in Example 1.

[0053] Comparative Example 3 The volume percentage formula of this comparative compound essential oil is exactly the same as that of Example 1: clove essential oil 45%, frankincense essential oil 15%, sandalwood essential oil 15%, litsea cubeba essential oil 10%, and myrrh essential oil 15%.

[0054] The difference in preparation method is that the mixture is stirred under normal pressure and open environment, while the other parameters such as temperature, stirring rate, time, maturation and filtration are the same as in Example 1.

[0055] Comparative Example 4 The volume percentage formulation of this comparative compound essential oil is completely consistent with that of Example 1.

[0056] The difference in the preparation method is that the stirring conditions in step three are changed to 200 r / min and the stirring time is 8 min, while the rest of the methods are the same as in Example 1.

[0057] Comparative Example 5 The volume percentage formulation of this comparative compound essential oil is completely consistent with that of Example 1.

[0058] The difference in the preparation method is that the curing conditions in step four are changed to 40°C, natural light irradiation environment, and curing time of 12h, while the rest of the methods are the same as in Example 1.

[0059] Comparative Example 6 The volume percentage formulation of this comparative compound essential oil is completely consistent with that of Example 1.

[0060] The difference in preparation method is that the step-by-step mixing process from step one to step four is cancelled. Clove essential oil, frankincense essential oil, sandalwood essential oil, litsea cubeba essential oil, and myrrh essential oil are mixed together in one go according to the formula. Stirred at 30℃ and 400r / min for 12min. There is no light-proof standing maturation step. Filter directly using a 0.22μm filter membrane. No other conditions are adjusted.

[0061] III. Safety and Skin Care Efficacy Tests The compound essential oils prepared in Examples 1-6 and Comparative Examples 1-6 were diluted with sweet almond oil as a base oil (dilution ratio of 1:100) to obtain essential oil samples for testing. The essential oil samples were then subjected to the following tests: (1) Security testing: Referring to the test methods in Chapter 7 of the "Cosmetic Safety Technical Specifications (2015 Edition)," a human skin patch test was conducted. One hundred volunteers (aged 20-55 years) were selected as subjects (excluding patients with insulin-dependent diabetes, patients undergoing treatment for asthma or other chronic respiratory diseases, those with highly sensitive constitutions, breastfeeding or pregnant women, etc.; all selected volunteers signed informed consent forms). The potential for adverse skin reactions caused by the compound essential oils in the above examples and comparative examples was tested. The specific testing procedure was as follows: 0.02 ml of essential oil sample was placed in the patch applicator chamber. Distilled water was used as a blank control. The patch applicator containing the sample was applied to the flexor side of the subject's forearm, and the palm was gently pressed to ensure even application to the skin for 24 hours. Skin reactions were observed and recorded according to the grading criteria in Table 2 30 minutes, 24 hours, and 48 hours after removing the patch applicator.

[0062] Table 2 Grading Criteria for Skin Reactions to Occlusive Patches

[0063] (2) Anti-wrinkle effect test: Following the "Test Method for Anti-wrinkle Efficacy of Cosmetics (T / ZHCA006-2019)," 360 Chinese female volunteers with crow's feet wrinkles ≥2 points (actual age between 35-62 years old, all qualified volunteers signed informed consent forms) were selected. The test area was free from significant interfering factors that could affect the test results, such as birthmarks, acne, scratches, moles, etc. Women who are breastfeeding, pregnant, or planning to have children were excluded; those with allergies, allergic dermatitis, or currently receiving dermatological treatment were excluded. The test environment temperature was (21.0±1.0)℃, and the test environment humidity was 50%±10%. Volunteers were randomly divided into 12 groups of 30 each. Each group used the essential oil samples from the examples and comparative examples for 4 consecutive weeks. At weeks 0, 2, and 4, photos were taken using the Primos-CR-3D skin analyzer (Note: Before taking photos, the facial skin was cleansed with water, and after cleansing, the volunteers sat quietly in a constant temperature and humidity room for 30 minutes before taking photos). The parameters of crow's feet, including number, depth, area, and length, were analyzed and the results were recorded (average values ​​were taken for each group).

[0064] (3) Skin brightening effect test: 360 volunteers were selected. Inclusion criteria: healthy Chinese women aged 18-45; no sensitivity to commonly used skincare products; high compliance. Exclusion criteria: those who had used antihistamines within the past week or immunosuppressants within the past month; those who had used any anti-inflammatory drugs on the test site within the past two months; insulin-dependent diabetic patients; patients currently receiving treatment for respiratory diseases; individuals with allergies, allergic dermatitis, or a history of skin diseases; those currently receiving dermatological treatment or who had taken hydroxy acids, whitening agents, or anti-aging drugs within the past month; and those with large areas of birthmarks, scratches, vitiligo, pigmented nevi, keloids, or other skin abnormalities that might affect the test. All selected volunteers signed an informed consent form.

[0065] Volunteers were randomly divided into 12 groups of 30 each. The test was conducted in a constant temperature and humidity environment (21.0±1.0℃, 50%±10%). Before the test, participants cleaned their faces as instructed and rested for 20 minutes in the environment. Facial images were captured using Visia-CR standard light as initial images before sample application. Each group of volunteers then received essential oil samples from the respective examples and comparative examples, at a concentration of 2 mg / cm³. 2 After applying the compound essential oil until it fully adheres to the skin, a second facial image is captured using Visia-CR. Eight hours after application, a third facial image is captured using Visia-CR.

[0066] Skin color analysis was performed on the standard light image above. The cheek or forehead area was selected in the AOI region. The Lab color channel of the computational macro was applied to obtain the Lab mean. The L value representing the brightness of the skin color was extracted. The ITA° value (individual type angle) representing the whiteness of the skin color was calculated and the results were recorded.

[0067] IV. Results Analysis (1) Security test results: All 100 volunteers completed the 24-hour closed skin patch test for each embodiment and comparative example. After removing the patch device for 30 minutes, 24 hours, and 48 hours, the essential oil samples of each embodiment and comparative example did not produce any adverse skin reactions, i.e., all were grade 0 negative reactions, indicating that the compound essential oil of this application has good skin safety.

[0068] (2) Results of anti-wrinkle effect test: The essential oil samples prepared according to Examples 1-6 of this application were evaluated after 4 weeks of continuous use, and the statistical results are as follows: Figure 2-5 As shown, the number, depth, area, and length of wrinkles all decreased significantly, with Example 1 showing the most outstanding anti-wrinkle effect.

[0069] The above content is further illustrated below by showing the initial data (week 0) and the data after four weeks of use, as shown in Tables 3 and 4.

[0070] Table 3 Results of anti-wrinkle effect test in week 0

[0071] Table 4. Results of anti-wrinkle effect test in week 4

[0072] A comparison of Tables 3 and 4 shows that the compound essential oils prepared in Examples 1-6 all exhibited significant anti-wrinkle effects, with Example 1 showing the most outstanding results. After 4 weeks, the number of wrinkles decreased from 12 to 7, the wrinkle depth decreased from 49 μm to 31 μm, and the wrinkle area decreased from 1.85 mm. 2 Reduced to 1.16mm 2 The wrinkle length was shortened from 22.5mm to 14.2mm, with all indicators decreasing by more than 40%. Other embodiments also achieved significant improvements in various wrinkle parameters.

[0073] Comparative Example 1, which did not include Litsea cubeba essential oil in its formula, showed a significantly worse anti-wrinkle effect compared to Example 1. After 4 weeks, Example 1 showed a reduction of 5 wrinkles and a decrease in wrinkle depth of 18 μm, while Comparative Example 1 only showed a reduction of 2 wrinkles and a decrease in wrinkle depth of 6 μm. The improvement in wrinkle area and length was significantly lower than that of Example 1. This indicates that Litsea cubeba essential oil, as one of the main components, contains active ingredients such as citral, which can work synergistically with other essential oils to exert antioxidant and free radical damage reduction effects. Without it, an effective anti-wrinkle synergistic system cannot be formed, resulting in a significant reduction in the anti-wrinkle effect.

[0074] The anti-wrinkle effect of Comparative Example 2 was also significantly worse than that of Example 1; after 4 weeks, the number of wrinkles decreased by only 2 and the depth decreased by 8μm. The improvement of various indicators was significantly lower than that of Example 1, and only slightly improved compared to Comparative Example 1. This shows that the optimal anti-wrinkle synergistic effect can only be achieved when the raw materials are within the limited ratio range. An imbalance in the ratio will lead to a disorder in the proportion of active ingredients and will not be able to fully exert the anti-wrinkle effect.

[0075] The anti-wrinkle effect of Comparative Example 3 was significantly worse than that of Example 1. Example 1 had a stable anti-wrinkle effect because nitrogen protection could effectively prevent the oxidation of essential oils. However, after 4 weeks, the number of wrinkles in Comparative Example 3 decreased by 3 and the depth decreased by 10 μm. The effect was worse than that of Example 1, but better than that of Comparative Example 1 and Comparative Example 2. This shows that nitrogen protection can prevent the active ingredients of essential oils from being oxidized and degraded. Without nitrogen protection, the content of active ingredients decreases and the anti-wrinkle effect declines significantly.

[0076] The anti-wrinkle effect of Comparative Example 4 was significantly worse than that of Example 1; after 4 weeks, the number of wrinkles decreased by 3 and the depth decreased by 9μm. The improvement of various indicators was slightly lower than that of Comparative Example 3, and only slightly improved compared to Comparative Example 1 and Comparative Example 2. This shows that appropriate stirring parameters can ensure that the essential oils are fully mixed and that the active ingredients are evenly dispersed. Insufficient stirring will lead to uneven mixing system, and the active ingredients cannot work synergistically, thus affecting the anti-wrinkle effect.

[0077] The anti-wrinkle effect of Comparative Example 5 was significantly worse than that of Example 1; after 4 weeks, the number of wrinkles decreased by only 2 and the depth decreased by 7μm, which was similar to that of Comparative Example 2, but worse than that of Comparative Example 3 and Comparative Example 4. This indicates that high temperature and light will accelerate the degradation of the active ingredients of essential oils, and shortening the curing time will prevent the active ingredients from fully fusing and reacting. The superposition of multiple process defects led to a significant decline in the anti-wrinkle effect.

[0078] The anti-wrinkle effect of Comparative Example 6 was significantly worse than that of Example 1; after 4 weeks, the number of wrinkles decreased by 3 and the depth decreased by 8μm, which was better than Comparative Examples 1, 2 and 5, but slightly worse than Comparative Examples 3 and 4. This shows that step-by-step mixing can allow the essential oils to gradually blend together, and the maturation step can promote the full reaction of active ingredients to form a stable anti-wrinkle system. Without both, the active ingredients cannot fully synergize, and the anti-wrinkle effect is significantly reduced.

[0079] (3) Skin brightening effect test results: The test results of Examples 1-6 and Comparative Examples 1-6 were compared. The test time was the initial state, the test immediately after use, and the test 8 hours after use. The results are shown in Tables 5, 6 and 7.

[0080] Table 5 Data table under initial conditions

[0081] Table 6 Data immediately after use

[0082] Table 7 Data after 8 hours of use

[0083] As can be seen from Tables 5 to 7, the essential oil samples prepared in Examples 1-6 of this application can immediately and significantly improve the whiteness and brightness of the skin. Moreover, after 8 hours of use, the skin can maintain a level that is basically the same as that immediately after use, indicating that the compound essential oil of this application can significantly brighten the skin tone. Among them, the brightening effect of Example 1 is the most outstanding. Immediately after use, the L value increased from 49.2 to 53.6 and the ITA° value increased from 42.1 to 47.8. After 8 hours, the values ​​were still maintained at 52.9 and 47.1, respectively, showing excellent skin brightening durability. The other examples showed an immediate increase in L value of 3.8-4.2 and an increase in ITA° value of 5.1-5.5 after use, and all maintained a high level after 8 hours.

[0084] The skin brightening effect of Comparative Example 1 was significantly worse than that of Example 1. Immediately after use, the L value of Comparative Example 1 only increased from 49.1 to 50.8 and the ITA° value increased from 42.0 to 43.7. After 8 hours, they quickly dropped back to 50.2 and 43.1, respectively. The improvement of each indicator was less than 40% of that of Example 1. This indicates that the active ingredients such as citral in Litsea cubeba essential oil can work synergistically with essential oils such as clove bud and frankincense to enhance the skin brightening effect. Without these ingredients, an effective skin brightening synergy system cannot be formed, resulting in a significant reduction in the skin brightening effect and a decrease in its lasting effect.

[0085] The brightening effect of Comparative Example 2 was significantly worse than that of Example 1; immediately after use, its L value increased from 48.9 to 51.2 and its ITA° value increased from 41.8 to 44.2, but dropped back to 50.6 and 43.6 after 8 hours. The brightening effect was slightly higher than that of Comparative Example 1, but still far worse than that of Example 1. This shows that the raw materials need to be combined within a limited ratio range to fully exert their synergistic brightening effect. An imbalance in the ratio will lead to disordered action of active ingredients and poor brightening effect.

[0086] The skin brightening effect of Comparative Example 3 was significantly worse than that of Example 1; immediately after use, its L value increased from 49.0 to 51.7 and its ITA° value increased from 41.9 to 44.8, and remained at 51.0 and 44.2 after 8 hours. The skin brightening effect was better than that of Comparative Example 1 and Comparative Example 2, but worse than that of Example 1. This indicates that nitrogen protection can prevent the oxidation of active ingredients in essential oils. Without nitrogen protection, the active ingredients degrade, leading to a decline in the skin brightening effect.

[0087] The skin brightening effect of Comparative Example 4 was significantly worse than that of Example 1; immediately after use, its L value increased from 48.8 to 51.4 and its ITA° value increased from 41.7 to 44.5, but dropped back to 50.8 and 43.9 after 8 hours. The skin brightening effect was slightly worse than that of Comparative Example 3, but better than that of Comparative Example 1 and Comparative Example 2. This shows that appropriate process parameters can ensure uniform dispersion of active ingredients. Insufficient stirring will lead to uneven mixing, and the active ingredients will not be able to work together to brighten the skin, thus affecting the effect.

[0088] The skin brightening effect of Comparative Example 5 was significantly worse than that of Example 1. Immediately after use, its L value increased from 48.7 to 50.9 and its ITA° value increased from 41.5 to 43.8. After 8 hours, it dropped back to 50.3 and 43.2. The skin brightening effect was similar to that of Comparative Example 1 but worse than that of other comparative examples. This indicates that high temperature, light exposure and shortened curing time will accelerate the degradation of active ingredients and prevent the formation of a stable skin brightening system. The superposition of multiple process defects led to a significant reduction in the skin brightening effect.

[0089] The brightening effect of Comparative Example 6 was significantly worse than that of Example 1; immediately after use, its L value increased from 48.8 to 51.1 and its ITA° value increased from 41.7 to 44.1, and remained at 50.5 and 43.5 after 8 hours. The brightening effect was better than that of Comparative Examples 1, 2 and 5, but slightly worse than that of Comparative Examples 3 and 4. This shows that stepwise mixing and maturation can promote the full fusion reaction of active ingredients. Without both, the synergistic effect of brightening is weakened and the effect decreases.

[0090] Therefore, by using the above-mentioned compound essential oil with anti-wrinkle effects, its preparation method and application, and by blending five essential oils in the disclosed proportions and optimizing the preparation process, the compound essential oil achieves a synergistic anti-wrinkle effect while taking into account product stability and gentleness, making it suitable for applications in skincare products and other fields.

[0091] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0092] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. Furthermore, those skilled in the art will recognize that, based on the ideas of the present invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A compound essential oil with anti-wrinkle effects, characterized in that, The raw materials of the compound essential oil, by volume percentage, include: Clove essential oil 20%-50%; Frankincense essential oil 5%-20%; Sandalwood essential oil 5%-20%; Litsea cubeba essential oil 5%-15%; Myrrh essential oil 10%-20%.

2. The compound essential oil with anti-wrinkle effects according to claim 1, characterized in that, The raw materials of the compound essential oil, by volume percentage, include: Clove essential oil 45%; Frankincense essential oil 15%; Sandalwood essential oil 15%; 10% Litsea cubeba essential oil; Myrrh essential oil 15%.

3. A method for preparing a compound essential oil with anti-wrinkle effects as described in claim 1 or 2, characterized in that, Includes the following steps: S1. Weigh sandalwood essential oil and myrrh essential oil according to the ratio, and stir at 15-25℃ until a homogeneous solution is obtained to obtain the first mixed phase; S2. Add pheasant oil to the first mixed phase and stir at 20-30℃ until a homogeneous solution is obtained to obtain the second mixed phase; S3. Add frankincense essential oil to the second mixed phase and mix evenly at 25-35℃ with stirring to obtain the third mixed phase; S4. Add clove essential oil to the third mixed phase, controlling the addition time to 5-15 minutes. After mixing, seal and let it stand in the dark at 25-30℃ for 24-48 hours to mature. After filtration, the compound essential oil is obtained.

4. The preparation method according to claim 3, characterized in that, In steps S1 and S2, the raw materials are mixed under nitrogen protection.

5. The preparation method according to claim 3, characterized in that, In step S3, the stirring conditions are stirring at a rate of 300-500 r / min for 10-15 minutes.

6. The preparation method according to claim 3, characterized in that, In step S4, the curing time is 36 hours.

7. The preparation method according to claim 3, characterized in that, In step S4, the filtration is performed using a filter membrane or filter paper with a pore size of 0.22μm-10μm.

8. The application of a compound essential oil with anti-wrinkle effects as described in claim 1 or 2 in skin care products.

9. The application according to claim 8, characterized in that, The compound essential oil is added to skin care products at a rate of 1%-3%.

10. The application according to claim 9, characterized in that, The skincare product is one of the following: facial oil, face cream, lotion, eye cream, or massage oil.

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