Female and child skin care composition and preparation method thereof

Abstract

The invention relates to a skin care composition for women and children and a preparation method thereof. The composition comprises the following components: tremella fuciformis extract nanocrystals, a calendula extract, an oat extract, dipotassium glycyrrhizinate, panthenol, sodium hyaluronate and the like. By using a nanocrystalline technology and a compound stabilizer, the moisturizing and anti-oxidation effects of the product are remarkably improved, and the product is suitable for various skins, especially sensitive skins. The product is safe and mild, effectively reduces skin irritation and improves the skin barrier function. The problems that an existing skin care product is not lasting in moisturizing effect and insufficient in oxidation resistance are solved, and an efficient and stable skin care solution is provided.

Description

Technical Field

[0001] This invention relates to the technical field of skin care compositions for women and children, and in particular to a skin care composition for women and children and its preparation method. Background Technology

[0002] In recent years, with the improvement of people's living standards and the enhancement of health awareness, the market demand for skincare products has grown rapidly. Especially in the women's and children's market segment, skincare products tailored to different age groups, skin types, and individual needs are constantly being innovated. Traditional skincare compositions mainly focus on basic functions such as moisturizing, hydrating, and anti-oxidation. However, with the development of modern science and technology, more and more research is focusing on the application of natural plant extracts, such as aloe vera, calendula, and tremella, to leverage their unique skincare effects. Furthermore, the introduction of advanced processes such as nanotechnology and bio-fermentation technology has greatly improved the stability and absorption efficiency of active ingredients in skincare products. These technological advancements have not only enriched the variety of products but also improved their safety and efficacy. However, despite the significant progress made in the skincare field, some problems still urgently need to be addressed.

[0003] The shortcomings of existing technologies significantly impact product efficacy and market competitiveness. Firstly, most skincare products suffer from limitations in moisturizing effects, struggling to achieve deep and long-lasting skin hydration. Traditional moisturizing ingredients such as glycerin and hyaluronic acid, while possessing some moisturizing properties, have relatively short penetration and retention times due to molecular structure limitations. Furthermore, while antioxidant skincare products can combat skin aging, the stability of their active ingredients and their delivery remain technical challenges. Especially for skincare products targeting children, current technologies primarily focus on gentleness and safety, but fall short in terms of functionality and specific product targeting.

[0004] Chinese patent (CN118267327A) discloses a water-locking and moisturizing transparent gel cream suitable for children and its preparation method. Although the existing technology enhances the moisturizing effect of the product by adding a variety of moisturizing ingredients, such as glycerin, diglycerin, and hydroxyethyl urea, some products still have the problem of insufficient moisturizing effect. Especially in low humidity environments, the moisturizing effect of some products is significantly weakened, which may be due to the unstable water-retaining film formed on the skin surface by the moisturizing ingredients.

[0005] In response to these challenges, we have invented an innovative skincare composition for women and children and its preparation method. By introducing nanocrystal technology of tremella extract and synergistic effects of multiple plant extracts, we have not only improved the moisturizing and antioxidant effects of the product, but also achieved breakthroughs in safety and targeted treatment, effectively making up for the shortcomings of existing technologies. Summary of the Invention

[0006] The shortcomings of existing technologies significantly impact product efficacy and market competitiveness. Firstly, most skincare products suffer from limitations in moisturizing effects, struggling to achieve deep and long-lasting skin hydration. Traditional moisturizing ingredients such as glycerin and hyaluronic acid, while possessing some moisturizing properties, have relatively short penetration and retention times due to molecular structure limitations. Furthermore, while antioxidant skincare products can combat skin aging, the stability of their active ingredients and their delivery remain technical challenges. Especially for skincare products targeting children, current technologies primarily focus on gentleness and safety, but fall short in terms of functionality and specific product targeting.

[0007] This application provides a skin care composition for women and children, comprising the following components by mass fraction: 0.5%-3.0% (w / w) of Tremella fuciformis extract nanocrystals, 0.5%-2.0% (w / w) of Calendula officinalis extract, 0.5%-2.0% (w / w) of Oat extract, 0.1%-0.5% (w / w) of Dipotassium glycyrrhizate, 0.1%-0.5% (w / w) of Panthenol, 0.1%-0.5% (w / w) of Sodium hyaluronate, 0.05%-0.5% (w / w) of Squalane, 2.0%-10.0% (w / w) of Vegetable Glycerin, 0.1%-0.5% (w / w) of Thickener, 0.5%-3.0% (w / w) of Vegetable Oil, 0.01%-0.1% (w / w) of Sodium Lactate or Sodium Citrate, and purified water to the balance up to 100%.

[0008] As a preferred technical solution for a skin care composition for women and children, the composition comprises the following components by mass fraction: Tremella fuciformis extract nanocrystals: 2.0% (w / w), Calendula officinalis extract: 1.5% (w / w), Oat extract: 1.5% (w / w), Dipotassium glycyrrhizate: 0.3% (w / w), Panthenol: 0.3% (w / w), Sodium hyaluronate: 0.3% (w / w), Squalane: 1.0% (w / w), Vegetable glycerin: 5.0% (w / w), Thickener: 0.3% (w / w), Vegetable oil: 2.0% (w / w), Sodium lactate or sodium citrate: 0.05% (w / w), and Purified water: balance to 100%.

[0009] It should be noted that Tremella fuciformis extract is rich in polysaccharides, possessing excellent moisturizing and antioxidant properties. Its nanocrystalline form enhances its bioavailability and skin permeability, allowing it to penetrate deep into the skin layers, increasing hydration and reducing moisture loss. Calendula extract has anti-inflammatory, antibacterial, and soothing effects, helping to relieve skin inflammation and discomfort, promoting skin healing, and is suitable for sensitive skin. Oat extract is rich in β-glucan, possessing moisturizing, antioxidant, and anti-inflammatory effects, capable of soothing dry and sensitive skin and enhancing skin barrier function. Dipotassium glycyrrhizate is a mild anti-inflammatory ingredient with anti-allergic and soothing effects, helping to reduce skin redness and inflammation. Panthenol is a moisturizing and repairing ingredient that promotes skin hydration, enhances skin barrier function, reduces skin inflammation and irritation, and improves skin softness. Sodium hyaluronate is a... This product contains several powerful moisturizers that absorb and retain moisture, increasing skin hydration and leaving it looking plumper and healthier. Squalane, a natural emollient with high skin compatibility, enhances skin softness and elasticity while forming a protective film on the skin's surface to reduce moisture loss. Vegetable glycerin, a common moisturizer, attracts and retains moisture, helping to soften the skin and improve its barrier function, especially effective in low-humidity environments. Thickeners such as xanthan gum and guar gum, used to increase product viscosity and stability, provide a smooth texture and good spreadability while helping to stabilize the suspension of active ingredients. Plant-based oils (such as sunflower seed oil), rich in unsaturated fatty acids and vitamin E, offer excellent moisturizing and antioxidant effects, repairing and maintaining the skin barrier function and preventing moisture loss. The nanocrystals of tremella extract are rich in polysaccharides, which have strong moisturizing and antioxidant properties; sodium hyaluronate is a powerful moisturizer that can absorb and retain a large amount of water; the combination of the two can significantly enhance the moisturizing effect of the product, prolong the moisturizing time, and reduce water loss by forming a moisturizing film. Calendula extract has anti-inflammatory and soothing properties, effectively relieving skin discomfort; oat extract is rich in beta-glucan, which has soothing and moisturizing effects; the combined effect of these two extracts can enhance the anti-inflammatory and soothing efficacy of the product, making it especially suitable for sensitive skin and helping to reduce skin irritation and redness. Dipotassium glycyrrhizate has anti-inflammatory and anti-allergic effects, which can relieve skin inflammation and allergic reactions. When combined with calendula extract, it can enhance the anti-inflammatory and repair functions of the product and help reduce skin redness and irritation. Squalane is a natural emollient that enhances the skin's softness and elasticity; vegetable glycerin is a common moisturizer with water-absorbing and water-locking abilities. The combination of the two provides long-lasting hydration while improving skin texture, leaving it soft, smooth, and non-greasy. Another inventive aspect of this invention provides a method for preparing a skincare composition for women and children, comprising the following preparation steps: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 300-500 rpm for 15-25 minutes until all components are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 40-50°C until completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 10-15 minutes using a high-speed homogenizer at 500-700 rpm to ensure that the oil and water phases are fully mixed and form a uniform emulsion. Step S4. While stirring, slowly add the thickener to ensure complete dispersion. Then slowly add the tremella extract nanocrystals to the emulsified matrix and stir at low speed until evenly distributed. Use a high-pressure homogenizer to homogenize the mixture, setting the pressure to 100-150 bar and repeating the process 3-5 times to ensure even dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust it to the range of 5.0-6.0 using sodium lactate or sodium citrate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

[0010] It should be noted that during the preparation of the aqueous base solution, low-speed stirring (300-500 rpm) ensured the uniform distribution of the components and prevented the formation of excessive bubbles, thus avoiding instability during the subsequent emulsification process. Calendula extract and oat extract, through their anti-inflammatory and soothing effects, combined with the anti-allergic properties of dipotassium glycyrrhizate, work together to address skin inflammation and irritation. Panthenol and sodium hyaluronate enhance the skin's moisturizing effect, while plant glycerin helps retain skin moisture through its hygroscopic properties. The process of heating squalane and plant oils to 40-50℃ in a water bath primarily utilizes thermodynamic principles; by increasing the temperature, the viscosity of the oils is reduced, making them more readily react with the aqueous phase. The mixture, consisting of squalane and plant oils, are nonpolar molecules with lipophilic properties. They form a protective film on the skin's surface, preventing moisture loss and providing a moisturizing and softening effect. The heating process also helps release and activate the active ingredients in the oils, such as the antioxidant effects of vitamin E, thus providing additional protection during skincare. Emulsification is the process of mixing two immiscible liquids (such as water and oil) to form a stable emulsion. Through continuous stirring and high-speed homogenization (500-700 rpm), the oil phase is dispersed into small droplets and evenly distributed in the aqueous phase. This process utilizes the action of surfactants. Lowering the surface tension at the oil-water interface allows oil droplets to exist stably in the aqueous phase. The stability of the emulsion is achieved through the formation of a stabilizing layer around the oil droplets. This stabilizing layer is formed by the interfacial interactions between water-soluble and fat-soluble components. High-speed homogenization further reduces the size of the oil droplets, improving the uniformity and stability of the emulsion. Thickeners (such as xanthan gum and guar gum) stabilize the emulsion and provide a suitable texture by increasing the viscosity of the solution and forming a three-dimensional network structure. They restrict the fluidity of the liquid through hydrogen bonds and other intermolecular interactions, giving the emulsion a certain consistency and stability. (Tremella fuciformis) The addition of extract nanocrystals utilizes nanotechnology, leveraging the high surface area and permeability of nanoparticles to more easily penetrate the skin barrier, exerting deep moisturizing and antioxidant effects. The use of a high-pressure homogenizer mechanically disperses the nanocrystals evenly in the matrix, reducing particle size and further improving stability and bioavailability. Sodium lactate or sodium citrate is used for pH adjustment, adjusting the solution's acidity and alkalinity through acid-base neutralization to ensure the product's gentleness and suitability. A suitable pH value not only reduces skin irritation but also enhances the stability and activity of other active ingredients.

[0011] A preferred technical solution for preparing a skin care composition for women and children includes the following steps in the preparation process of the Tremella fuciformis extract nanocrystals: Weigh out phosphatidylcholine and polyglutamic acid, mix them, add an appropriate amount of purified water and stir until uniform to form a uniform dispersion system, thus obtaining a composite stabilizer solution. Add the tremella extract to the purified water and stir until completely dissolved. Add the composite stabilizer solution to the mixture and stir until uniform. The mixed solution was placed in an ultrasonic device with an ultrasonic frequency of 25-30 kHz and an ultrasonic time of 15-20 minutes. After ultrasonic treatment, the solution was immediately cooled to room temperature. During the cooling process, it was gently stirred. The cooled nanocrystalline solution was then subjected to high-pressure homogenization at a pressure of 120-150 bar for 3 times. The solution was then filtered using a 0.2-micron microporous membrane to remove residual macromolecules and impurities.

[0012] It should be noted that in the preparation of Tremella fuciformis extract nanocrystals, a composite stabilizer solution is first prepared by mixing phosphatidylcholine and polyglutamic acid, utilizing the unique properties of these two components to form a dual stabilization mechanism. Phosphatidylcholine is an amphiphilic molecule that can self-assemble into micelles or liposomes in aqueous solution, thereby encapsulating the active ingredient and preventing its aggregation. Polyglutamic acid, as a natural polypeptide, forms a stable three-dimensional network with other molecules through electrostatic interactions and hydrogen bonds, further enhancing the stability of the nanocrystals. The Tremella fuciformis extract is dissolved in purified water, and stirring ensures uniform dispersion, providing a stable solution basis for the subsequent nano-sizing process.

[0013] Next, using ultrasonic processing technology, the cavitation effect generated when ultrasound propagates in a liquid is utilized to disperse the tremella extract into nanoscale particles through localized high temperature, high pressure, and intense shear force. The frequency and time of ultrasonic processing are precisely controlled to ensure the tremella extract is fully nanoscaled while avoiding molecular structure damage caused by over-processing. The subsequent cooling process rapidly lowers the solution temperature, preventing thermal effects on the active ingredients and maintaining the structural stability of the nanocrystals.

[0014] Subsequently, high-pressure homogenization further refines and uniformly disperses the nanocrystalline particles. The high-pressure homogenizer operates at 120-150 bar, utilizing narrow channels to achieve high shear force and strong turbulence, further reducing the particle size of the nanocrystals and achieving a uniform distribution. Multiple homogenization processes ensure that all nanocrystalline particles reach the ideal size range, avoiding the presence of large particles and ensuring product stability and bioavailability.

[0015] Finally, the solution is filtered through a 0.2-micron microporous membrane to remove large molecular impurities and incompletely nano-sized particles. This step not only ensures the purity and stability of the nanocrystalline solution but also effectively removes impurities that may cause adverse reactions. The parameters of each step in the entire preparation process are strictly controlled to ensure the efficient stabilization of the Tremella fuciformis extract nanocrystals, ultimately yielding a nanocrystalline solution with uniform particle size and high stability, providing highly effective active ingredient support for skincare products.

[0016] In a preferred embodiment of a method for preparing a skin care composition for women and children, the thickener is xanthan gum or guar gum, and the vegetable oil is sunflower seed oil.

[0017] It should be noted that xanthan gum and guar gum, as thickeners, are mainly used to increase the viscosity and stability of skincare compositions, ensuring uniform product distribution and providing a good texture. They can form a stable colloidal structure, preventing layering. Sunflower seed oil is a lightweight plant-based oil rich in unsaturated fatty acids and vitamin E, offering excellent moisturizing and antioxidant properties. It forms a protective film on the skin's surface, locking in moisture and preventing dryness, making it suitable for all skin types, especially sensitive skin.

[0018] In a preferred embodiment of a method for preparing a skin care composition for women and children, the mass ratio of phosphatidylcholine to polyglutamic acid is 1:1.

[0019] It should be noted that the mass ratio of phosphatidylcholine to polyglutamic acid is 1:1, ensuring a balanced presence of both in the composite stabilizer. This ratio helps to form a stable nanocrystalline structure, with phosphatidylcholine providing protection and polyglutamic acid increasing stability and preventing nanocrystal aggregation and sedimentation.

[0020] In a preferred embodiment of a method for preparing a skin care composition for women and children, the thickener is a mixture of xanthan gum and guar gum.

[0021] It should be noted that xanthan gum is a polysaccharide produced by glucose fermentation, possessing a unique helical structure and strong intermolecular hydrogen bonding. It can form a high-viscosity solution in water, providing excellent thickening and suspension stability. Guar gum is a polysaccharide extracted from guar beans, its main component being galactomannan. Its molecular structure has a strong hydration effect, capable of absorbing large amounts of water to form a viscous solution. Although the two have different molecular structures, both possess excellent thickening capabilities in water. Xanthan gum provides a robust network structure, while guar gum increases the system's flexibility and hydration capacity. This complementarity allows the mixture to form a stable and homogeneous colloidal system.

[0022] The skincare composition for women and children provided in this application achieves significant moisturizing and antioxidant effects by combining tremella fuciformis extract nanocrystals, various plant extracts, and other highly effective moisturizing ingredients. The application of nanocrystal technology improves the bioavailability and stability of the active ingredients, ensuring good absorption and long-lasting effects on the skin. This product is particularly suitable for sensitive skin, effectively relieving skin irritation and inflammation while also exhibiting good safety. The use of a complex stabilizer further enhances the product's uniformity and stability, resulting in excellent long-lasting moisturizing and antioxidant properties. Attached Figure Description

[0023] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings: Figure 1 Particle size distribution of the tremella extract nanocrystals prepared in Examples 1 to 3; Figure 2 This is a picture of the product prepared in Example 3. Detailed Implementation

[0024] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0025] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0026] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0027] Preparation Example 1: Preparation of Tremella fuciformis extract nanocrystals The specific steps of the preparation process of tremella extract nanocrystals include the following: weigh phosphatidylcholine and polyglutamic acid in a mass ratio of 1:1 and mix them. Add an appropriate amount of purified water and stir evenly. Add tremella extract to purified water and stir until completely dissolved. Add the composite stabilizer solution and mix evenly. The mixed solution was placed in an ultrasonic device with an ultrasonic frequency of 30 kHz and an ultrasonic time of 15 minutes. After ultrasonic treatment, the solution was immediately cooled to room temperature. During the cooling process, it was gently stirred. The cooled nanocrystalline solution was subjected to high-pressure homogenization at a pressure of 150 bar and homogenized 3 times. The solution was then filtered using a 0.2-micron microporous membrane to remove residual macromolecules and impurities.

[0028] Preparation Example 2 The difference between this preparation example and Preparation Example 1 is that an equal amount of phosphatidylcholine was weighed as a stabilizer.

[0029] Preparation Example 3 The difference between this preparation example and Preparation Example 1 is that an equal amount of polyglutamic acid was weighed as a stabilizer.

[0030] Example 1 A skincare composition for women and children comprises the following components by weight percentage: 0.5% (w / w) of Tremella fuciformis extract nanocrystals, 2.0% (w / w) of Calendula officinalis extract, 1.0% (w / w) of Oat extract, 0.5% (w / w) of Dipotassium glycyrrhizate, 0.1% (w / w) of Panthenol, 0.2% (w / w) of Sodium hyaluronate, 1.0% (w / w) of Squalane, 10.0% (w / w) of Vegetable Glycerin, 0.5% (w / w) of Xanthan Gum, 1.0% (w / w) of Sunflower Seed Oil, 0.1% (w / w) of Sodium Lactate, and purified water to the remainder up to 100%.

[0031] A method for preparing a skin care composition for women and children includes the following preparation steps: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 500 rpm for 15 minutes until all ingredients are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 40°C until completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 15 minutes at 500 rpm using a high-speed homogenizer to ensure that the oil and water phases are fully mixed to form a uniform emulsion. Step S4. While stirring, slowly add xanthan gum to ensure complete dispersion, then slowly add the tremella extract nanocrystals from Preparation Example 1 to the emulsified matrix, stir at low speed until uniformly distributed, and homogenize using a high-pressure homogenizer at a pressure of 150 bar for 5 times to ensure uniform dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust the pH value to the range of 6.0 using sodium lactate or sodium citrate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

[0032] Example 2 A skincare composition for women and children comprises the following components by weight percentage: 3.0% (w / w) of Tremella fuciformis extract nanocrystals, 0.5% (w / w) of Calendula officinalis extract, 2.0% (w / w) of Oat extract, 0.3% (w / w) of Dipotassium glycyrrhizate, 0.4% (w / w) of Panthenol, 0.5% (w / w) of Sodium hyaluronate, 2.0% (w / w) of Squalane, 2.0% (w / w) of Vegetable Glycerin, 0.1% (w / w) of Guar Gum, 0.5% (w / w) of Sunflower Seed Oil, 0.1% (w / w) of Sodium Lactate, and purified water to the remainder up to 100%.

[0033] A method for preparing a skin care composition for women and children includes the following preparation steps: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 300 rpm for 25 minutes until all ingredients are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 50°C until completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 10 minutes at 600 rpm using a high-speed homogenizer to ensure that the oil and water phases are fully mixed to form a uniform emulsion. Step S4. While stirring, slowly add guar gum to ensure complete dispersion, then slowly add the tremella extract nanocrystals prepared in Preparation Example 1 to the emulsified matrix, stir at low speed until uniformly distributed, and use a high-pressure homogenizer to homogenize the components. The pressure is set at 100 bar and the process is repeated 5 times to ensure uniform dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust the pH value to the range of 5.0 using sodium lactate or sodium citrate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

[0034] Example 3 A skincare composition for women and children comprises the following components by weight percentage: 2.0% (w / w) of Tremella fuciformis extract nanocrystals, 1.5% (w / w) of Calendula officinalis extract, 1.5% (w / w) of Oat extract, 0.3% (w / w) of dipotassium glycyrrhizate, 0.3% (w / w) of panthenol, 0.3% (w / w) of sodium hyaluronate, 1.0% (w / w) of squalane, 5.0% (w / w) of vegetable glycerin, 0.3% (w / w) of a mixture of xanthan gum and guar gum, 2.0% (w / w) of sunflower seed oil, 0.05% (w / w) of sodium citrate, and purified water to the remainder up to 100%.

[0035] A method for preparing a skin care composition for women and children includes the following preparation steps: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 300 rpm for 25 minutes until all ingredients are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 50°C until completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 15 minutes at 700 rpm using a high-speed homogenizer to ensure that the oil and water phases are fully mixed to form a uniform emulsion. Step S4. While stirring, slowly add the mixture of xanthan gum and guar gum to ensure complete dispersion. Then slowly add the tremella extract nanocrystals prepared in Preparation Example 1 to the emulsified matrix and stir at low speed until uniformly distributed. Use a high-pressure homogenizer to homogenize the mixture at a pressure of 150 bar for 3 times to ensure uniform dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust the pH value to the range of 6.0 using sodium citrate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

[0036] Example 4 A skincare composition for women and children comprises the following components by weight percentage: 2.0% (w / w) of Tremella fuciformis extract nanocrystals, 2.0% (w / w) of Calendula officinalis extract, 0.5% (w / w) of Oat extract, 0.1% (w / w) of Dipotassium glycyrrhizate, 0.5% (w / w) of Panthenol, 0.05% (w / w) of Sodium hyaluronate, 0.1% (w / w) of Squalane, 5.0% (w / w) of Vegetable Glycerin, 0.5% (w / w) of Xanthan Gum, 3.0% (w / w) of Sunflower Seed Oil, 0.01% (w / w) of Sodium Citrate, and purified water to the remainder up to 100%.

[0037] A method for preparing a skin care composition for women and children includes the following preparation steps: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 300 rpm for 20 minutes until all ingredients are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 50°C until completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 15 minutes at 600 rpm using a high-speed homogenizer to ensure that the oil and water phases are fully mixed to form a uniform emulsion. Step S4. While stirring, slowly add guar gum to ensure complete dispersion, then slowly add the tremella extract nanocrystals prepared in Preparation Example 1 to the emulsified matrix, stir at low speed until uniformly distributed, and homogenize using a high-pressure homogenizer at a pressure of 150 bar for 5 times to ensure uniform dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust the pH value to the range of 5.0 using sodium lactate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

[0038] Compare with Example 1 The difference between this comparative example and Example 3 is that an equal amount of Tremella fuciformis extract was used instead of Tremella fuciformis extract nanocrystals.

[0039] Compare with Example 2 The difference between this comparative example and Example 3 is that the tremella extract nanocrystals prepared in Preparation Example 2 were added to the emulsified matrix.

[0040] Compare with Example 3 The difference between this comparative example and Example 3 is that the tremella extract nanocrystals prepared in Example 3 were added to the emulsified matrix.

[0041] Experimental Example 1 Particle size distribution test: Sample preparation: Dissolve the extract in a suitable solvent to prepare a solution, ensuring the sample concentration is suitable for DLS measurement. Filter the sample using a 0.22 µm or 0.45 µm filter membrane to remove large particles or impurities and avoid interfering with the measurement. Treat the sample with an ultrasonic bath to remove air bubbles and ensure measurement accuracy.

[0042] Instrument calibration: Calibrate the DLS instrument using standard particles of known size (such as polystyrene microspheres) to ensure measurement accuracy.

[0043] Measurement setup: Inject the sample into a clean quartz sample cell and install it in the sample slot of the DLS instrument. Set the measurement parameters, including laser wavelength, detection angle, measurement time, and data acquisition frequency. The commonly used laser wavelength is 633 nm and the detection angle is 173°.

[0044] Data on particle size distribution of preparation examples 1 to 3 are as follows Figure 1 As shown.

[0045] from Figure 1 As can be seen, Preparation Example 1, which uses a combination of phosphatidylcholine and polyglutamic acid, exhibits the smallest particle size and narrowest particle size distribution, indicating the best control and stability of the particles. This is because the composite stabilizer provides a dual stabilizing mechanism, effectively preventing particle aggregation. Preparation Examples 2 and 3, on the other hand, show wider particle size distributions and poorer stability, indicating that single stabilizers have limited effectiveness in inhibiting particle aggregation. Therefore, the use of composite stabilizers has significant advantages in controlling nanoparticle size and improving dispersion stability.

[0046] Experiment Example 2 Skin moisture content measurement 1. Preparation Phase Skin tissue pretreatment: Standardized skin tissue is removed from storage conditions (such as cryopreservation) and equilibrated at room temperature for at least 30 minutes.

[0047] If using a reconstituted skin model, equilibrate it in an appropriate culture medium for at least 4 hours to ensure the tissue is in optimal condition before the experiment.

[0048] Environmental control: Experiments are conducted in a constant temperature and humidity environment, typically with the temperature set at 20-25℃ and the humidity maintained at 40%-60%.

[0049] 2. Initial Measurement Initial moisture content determination: The initial moisture content of the skin tissue surface was measured using a skin moisture meter (such as a Corneometer). Measurements were taken at multiple points (at least three points) for each skin sample, and the average value was calculated.

[0050] Record the measurement data as baseline values.

[0051] Initial TEWL measurement: Initial transdermal water loss was measured using a TEWL meter (such as the Tewameter) as baseline data for skin barrier function.

[0052] 3. Application testing products Apply moisturizing products: Using a pipette or other quantitative tool, apply the test product evenly to the skin tissue surface. Typically, use the same dose of product for each sample to ensure uniform coverage.

[0053] Gently massage (if applicable) to ensure full absorption of the product.

[0054] Blank group: A blank control group was also established, which used no product or a matrix without active ingredients, as a comparison.

[0055] 4. Subsequent measurements Time point measurement: At the designated time points (e.g., 30 minutes, 1 hour, 4 hours, 8 hours), measure the skin moisture content again. Measure multiple times at each time point and take the average value.

[0056] Record the measurement data at each time point.

[0057] Table 1 shows the moisturizing performance data. As can be seen from Examples 1 to 4 and Table 1, all examples exhibited significant moisturizing effects during the test, especially after the initial 30 minutes, when the moisture content reached approximately 70%. In the subsequent 1 to 8 hours, although the moisture content decreased slightly, it remained at approximately 65%, indicating its outstanding long-lasting moisturizing ability.

[0058] As can be seen from Example 3, Comparative Example 1, and Table 1, the moisturizing effect of Example 3 is significantly better than that of Comparative Example 1. Whether it is the immediate moisturizing ability or the long-lasting moisturizing effect, Example 3 successfully achieved a highly efficient moisturizing effect by optimizing the particle size of nanocrystals, surface modification, the use of composite stabilizers, and the control of the preparation process.

[0059] As can be seen from Example 3, Comparative Examples 2 and 3, and Table 1, the moisturizing effect of Example 3 is significantly better than that of Comparative Examples 2 and 3. Whether in terms of immediate or long-lasting effects, the composite stabilizer demonstrates a significant advantage in the preparation of nanocrystals, effectively controlling the particle size and improving stability. This superior particle size control and dispersion stability directly enhance the moisturizing effect of the nanocrystals, enabling them to better penetrate the skin, form a uniform moisturizing barrier, and provide long-lasting hydration. Therefore, using composite stabilizers is an important strategy for improving the moisturizing effect of nanocrystal products, while single stabilizers are relatively inferior in these aspects and cannot provide the same moisturizing performance. This finding provides valuable reference for the research and development and formulation optimization of high-efficiency skincare products.

[0060] Experimental Example 3 DPPH free radical scavenging experiment Principle: DPPH (2,2-diphenyl-1-picrylhydrazine radical) is a stable free radical with a purple color. When DPPH encounters an antioxidant, the free radical is reduced, the solution color lightens, and the absorbance decreases.

[0061] method: To prepare a DPPH solution, the concentration is usually 0.1 mM.

[0062] Mix the sample with the DPPH solution and react in the dark for a certain period of time (usually 30 minutes).

[0063] The decrease in absorbance of the mixture is measured using a UV-Vis spectrophotometer (typically at 517 nm).

[0064] The formula for calculating the DPPH radical scavenging rate is as follows: in, The absorbance is the absorbance without the addition of the sample. The absorbance is the value after the sample is added.

[0065] Table 2 shows the antioxidant performance data. As can be seen from Examples 1 to 4 and Table 2, the DPPH free radical scavenging rates of Examples 1 to 4 were 85%, 82%, 80%, and 78%, respectively. These data indicate that the Example group has strong antioxidant capacity, can effectively scavenge free radicals, and reduce the damage of oxidative stress to the skin. The formulation of the Example group may contain a variety of antioxidant ingredients, such as Tremella fuciformis extract nanocrystals, Calendula officinalis extract, and dipotassium glycyrrhizate. These ingredients work synergistically through different mechanisms to effectively enhance antioxidant performance.

[0066] As can be seen from Example 3, Comparative Example 1 and Table 2, the application of nanocrystal technology in skincare products significantly improves the antioxidant performance of the products. Nanocrystal technology enhances the bioavailability and efficacy of active ingredients by improving their permeability and stability. The performance of Comparative Example 1 is relatively weak, indicating the inadequacy of traditional non-nano-based ingredients in skincare effects.

[0067] As can be seen from Example 3, Comparative Examples 2 and 3, and Table 2, the antioxidant performance of Example 3 is significantly better than that of Comparative Examples 2 and 3. The application of composite stabilizers in the preparation of nanocrystals is significantly better than that of single stabilizers, especially in controlling the nanocrystal particle size and enhancing the antioxidant effect. By using composite stabilizers, smaller and more uniform nanocrystals can be obtained, enhancing their permeability and stability on the skin, thereby improving the bioavailability and efficacy of antioxidant ingredients. This advantage makes composite stabilizers an important component of highly effective antioxidant skincare products, further optimizing the overall performance of the product. Single stabilizers, due to the lack of synergistic effect, are weaker in terms of nanocrystal particle size control and antioxidant effect, demonstrating the application value of composite stabilizers in nanotechnology.

[0068] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skincare composition for women and children, characterized in that, The components, by mass fraction, include the following: Tremella fuciformis extract nanocrystals: 0.5%-3.0% (w / w), Calendula officinalis extract: 0.5%-2.0% (w / w), Oat extract: 0.5%-2.0% (w / w), Dipotassium glycyrrhizate: 0.1%-0.5% (w / w), Panthenol: 0.1%-0.5% (w / w), Sodium hyaluronate: 0.05%-0.5% (w / w), Squalane: 0.1%-2.0% (w / w), Vegetable glycerin: 2.0%-10.0% (w / w), Thickener: 0.1%-0.5% (w / w), Vegetable oil: 0.5%-3.0% (w / w), Sodium lactate or sodium citrate: 0.01%-0.1% (w / w), and Purified water: balance to 100%.

2. The skin care composition for women and children according to claim 1, characterized in that, The components, by weight fraction, are as follows: Tremella fuciformis extract nanocrystals: 2.0% (w / w), Calendula officinalis extract: 1.5% (w / w), Oat extract: 1.5% (w / w), Dipotassium glycyrrhizate: 0.3% (w / w), Panthenol: 0.3% (w / w), Sodium hyaluronate: 0.3% (w / w), Squalane: 1.0% (w / w), Vegetable glycerin: 5.0% (w / w), Thickener: 0.3% (w / w), Vegetable oil: 2.0% (w / w), Sodium lactate or sodium citrate: 0.05% (w / w), and Purified water: balance to 100%.

3. A method for preparing a skin care composition for women and children, characterized in that, The preparation steps include the following: Step S1. In a clean container, dissolve calendula extract, oat extract, dipotassium glycyrrhizate, panthenol, sodium hyaluronate, and vegetable glycerin in a portion of purified water. Stir at 300-500 rpm for 15-25 minutes until all components are completely dissolved to form an aqueous base solution. Step S2. Heat squalane and vegetable oil in a water bath to 40-50°C until they are completely dissolved and uniformly mixed to form an oil phase; Step S3. While stirring continuously, gradually add the oil phase to the water phase and mix for 10-15 minutes using a high-speed homogenizer at 500-700 rpm to ensure that the oil and water phases are fully mixed and form a uniform emulsion. Step S4. While stirring, slowly add the thickener to ensure complete dispersion. Then slowly add the tremella extract nanocrystals to the emulsified matrix and stir at low speed until evenly distributed. Use a high-pressure homogenizer to homogenize the mixture, setting the pressure to 100-150 bar and repeating the process 3-5 times to ensure even dispersion of the components. Step S5. Measure the pH value of the emulsion and adjust it to the range of 5.0-6.0 using sodium lactate or sodium citrate. Stir slowly to ensure that the pH adjuster is fully mixed and the solution is homogeneous.

4. The method for preparing the skin care composition for women and children according to claim 3, characterized in that, The preparation process of the tremella extract nanocrystals includes the following steps: Weigh out phosphatidylcholine and polyglutamic acid, mix them, add an appropriate amount of purified water and stir well. Add the tremella extract to the purified water and stir until completely dissolved. Add the composite stabilizer solution to the mixture. Mix thoroughly; The mixed solution was placed in an ultrasonic device with an ultrasonic frequency of 25-30 kHz and an ultrasonic time of 15-20 minutes. After ultrasonic treatment, the solution was immediately cooled to room temperature. During the cooling process, it was gently stirred. The cooled nanocrystalline solution was subjected to high-pressure homogenization at a pressure of 120-150 bar for 3 times. The solution was then filtered using a 0.2-micron microporous membrane to remove residual macromolecules and impurities.

5. The method for preparing the skin care composition for women and children according to claim 3, characterized in that, The thickener is xanthan gum or guar gum, and the vegetable oil is sunflower seed oil.

6. The method for preparing the skin care composition for women and children according to claim 4, characterized in that, The mass ratio of phosphatidylcholine to polyglutamic acid is 1:

1.

7. The method for preparing the skin care composition for women and children according to claim 3, characterized in that, The thickener is a mixture of xanthan gum and guar gum.

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