Compositions for improving skin hydration and antioxidant properties, and methods of making and using the same

Abstract

The application provides a composition for improving skin moisture and antioxidation, a preparation method and application thereof, and relates to the field of food technology.The composition according to the application is composed of the following components in parts by weight: 35-75 parts of tremella polysaccharide, 40-80 parts of sodium hyaluronate, 20-40 parts of collagen tripeptide, 20-40 parts of opuntia dillenii extract, 5-15 parts of aronia melanocarpa powder, 5-15 parts of sacha inchi oil, 10-30 parts of medlar original paste and 1-3 parts of vitamin C; the opuntia dillenii extract is obtained by juicing opuntia dillenii, treating with lactobacillus acidophilus, lactic acid bacteria and cellulase and subcritical water extraction treatment, and has a high yield.The composition according to the application has significant water and moisture retention, increased skin elasticity and antioxidation performance.

Description

Technical Field

[0001] This invention belongs to the field of food technology, specifically relating to compositions for improving skin hydration and anti-oxidation, their preparation methods, and applications. Background Technology

[0002] As consumers shift their understanding of skin health from exogenous care to endogenous conditioning, the market for food products with "oral beauty" functions is rapidly developing. The healthy appearance of skin, especially its hydration, elasticity, and radiance, fundamentally depends on its ability to retain moisture and resist oxidative damage. The integrity of the stratum corneum, the content of natural moisturizing factors, and the state of the dermal matrix (such as hyaluronic acid and collagen) collectively determine the skin's "water retention" capacity. Excessive reactive oxygen species generated by environmental factors such as ultraviolet radiation and pollution attack skin cells, accelerating hyaluronic acid degradation, collagen cross-linking, and lipid peroxidation, leading to dry skin, loss of elasticity, and aging.

[0003] Currently, sodium hyaluronate is a hot research topic in this field. Orally administered hyaluronic acid can be absorbed and distributed to the skin, increasing the hyaluronic acid content in the dermis and epidermis, thereby improving skin hydration. In addition, some natural plant extracts, such as tremella polysaccharide, aloe vera extract, and cactus fruit extract, are rich in polysaccharides and polyphenols, and have shown properties such as promoting hyaluronic acid synthesis or anti-inflammatory and moisturizing effects in vitro or in animal models. Therefore, sodium hyaluronate is often used in combination with plant polysaccharide and plant extract ingredients in existing food products.

[0004] For example, Chinese invention patent CN114287631A states that small-molecule sodium hyaluronate with an average molecular weight of 80KDa-800KDa has a significant effect on increasing skin hydration. Furthermore, both collagen peptides and small-molecule sodium hyaluronate are small molecules, making them easily absorbed by the human body. A composition made by mixing hydrolysate of collagen peptides, hydrolysate of small-molecule sodium hyaluronate, elastin, bird's nest powder, mangosteen powder, salmon nasal cartilage powder, purple chrysanthemum powder, astaxanthin, tremella polysaccharide, vitamin C, and concentrated fruit juice can improve skin aging, restore skin elasticity, increase skin moisture content, reduce skin moisture evaporation, increase procollagen secretion, promote collagen biosynthesis, and alleviate skin redness and sensitivity symptoms.

[0005] Chinese invention patent CN119769734A discloses a composition and its preparation method that can improve the skin's water retention capacity. The preparation method includes the following steps: preparing starch-salmon nasal cartilage composite nanopowder; preparing flavored moisturizing Tremella fuciformis polysaccharide fermentation broth; and combining salmon nasal cartilage light enzymatic hydrolysate with Tremella fuciformis polysaccharide fermentation broth. The flavored moisturizing Tremella fuciformis polysaccharide fermentation broth, salmon nasal cartilage powder light enzymatic hydrolysate, maltodextrin, Kanzan cherry blossom extract, and low-methoxyl pectin are mixed evenly and then freeze-dried under vacuum to obtain the composition that can improve the skin's water retention capacity.

[0006] However, the low absorption rate of high molecular weight sodium hyaluronate or polysaccharide (extract) components during oral administration affects their water-retention or antioxidant properties. Furthermore, existing moisturizing solutions are often separated from antioxidant solutions. In fact, oxidative stress is a core driver of skin moisturizing components (such as hyaluronic acid and collagen), barrier lipid peroxidation, and increased inflammation. Without fundamentally alleviating oxidative stress, moisturizing effects are like "building a tower on sand"—unsustainable. Moreover, existing compositions rarely simultaneously stimulate the synthesis of endogenous moisturizing factors and skin hyaluronic acid. This lack of systemic action limits their overall efficacy in comprehensively improving skin hydration and resisting environmental damage.

[0007] Therefore, it is necessary to develop a composition that can simultaneously target the two core mechanisms of "moisturizing" and "antioxidation," making the ingredient design more scientific, reflecting a targeted synergistic mechanism, and effectively addressing the damage of oxidative stress to the skin's moisturizing system. Summary of the Invention

[0008] This invention addresses the problems existing in the prior art by providing a composition for improving skin hydration and anti-oxidation, as well as its preparation method and application.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] First, the present invention provides a composition for improving skin hydration and anti-oxidation, comprising, by weight, the following components: 35-75 parts of Tremella fuciformis polysaccharide, 40-80 parts of sodium hyaluronate, 20-40 parts of collagen tripeptide, 20-40 parts of prickly pear cactus extract, 5-15 parts of black chokeberry powder, 5-15 parts of Sacha indica oil, 10-30 parts of wolfberry puree, and 1-3 parts of vitamin C;

[0011] The preparation method of the pear cactus extract is as follows:

[0012] (1) Mix pear cactus with water, juice it, filter it, and obtain filtrate A and residue A;

[0013] (2) Filter residue A is mixed with water and treated with Lactobacillus acidophilus, lactic acid bacteria and cellulase, and then filtered to obtain filtrate B and filter residue B;

[0014] (3) Filter residue B is extracted in stages using a subcritical water extraction system with water as the solvent:

[0015] First stage: Extraction at 110-130℃ and pressure 2.0-4.0MPa, followed by filtration to obtain filtrate C and filter residue C;

[0016] Second stage: Extraction at 140-160℃ and pressure 4.5-5.5MPa, followed by filtration to obtain filtrate D and filter residue D;

[0017] (4) Combine filtrate A, filtrate B, filtrate C and filtrate D to obtain pear cactus extract.

[0018] Preferably, the composition comprises, by weight, the following components: 40-60 parts of Tremella fuciformis polysaccharide, 50-70 parts of sodium hyaluronate, 25-35 parts of collagen tripeptide, 25-35 parts of prickly pear cactus extract, 8-12 parts of black chokeberry powder, 8-12 parts of Sacha indica oil, 15-25 parts of wolfberry puree, and 1.5-2.5 parts of vitamin C.

[0019] More preferably, the composition comprises, by weight, the following components: 50 parts of Tremella fuciformis polysaccharide, 60 parts of sodium hyaluronate, 30 parts of collagen tripeptide, 30 parts of prickly pear cactus extract, 10 parts of black chokeberry powder, 10 parts of Sacha indica oil, 20 parts of wolfberry puree, and 2 parts of vitamin C.

[0020] Preferably, the molecular weight of the sodium hyaluronate is 200,000 to 400,000.

[0021] Preferably, in step (1), the solid-liquid ratio of pear cactus and water during juicing is 1g:20-30mL.

[0022] More preferably, in step (1), the solid-liquid ratio of pear cactus and water during juicing is 1g:25mL.

[0023] Preferably, the juicing in step (1) is performed at 300-500W for 3-10 minutes.

[0024] More preferably, the juicing in step (1) is performed at 400W for 5 minutes.

[0025] Preferably, in step (2), the filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:10-20mL, wherein the mass of the solid is based on the pear cactus in step (1).

[0026] More preferably, in step (2), the filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:15mL.

[0027] Preferably, in step (2), the mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:2-4; more preferably, in step (2), the mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:3.

[0028] Preferably, in step (2), the total amount of Lactobacillus acidophilus and lactic acid bacteria used is 5%-10% of the mass of the pear cactus.

[0029] More preferably, in step (2), the total amount of Lactobacillus acidophilus and lactic acid bacteria used is 8% of the mass of the pear cactus.

[0030] Preferably, in step (2), the amount of cellulase used is 50-150 U / g, and the substrate is prickly pear cactus.

[0031] More preferably, in step (2), the amount of cellulase used is 100 U / g, and the substrate is prickly pear cactus.

[0032] Preferably, in step (2), the treatment conditions are: treatment at 34-40℃ for 12-48 hours.

[0033] More preferably, in step (2), the treatment conditions are: treatment at 36°C for 24 hours.

[0034] Preferably, in step (3), the water flow rate during the staged extraction is 2-4 mL / min; more preferably, in step (3), the water flow rate during the staged extraction is 3 mL / min.

[0035] Preferably, in step (3), the conditions of the first stage are: 110-130℃, 2.0-4.0MPa, extraction for 10-20min.

[0036] More preferably, in step (3), the conditions of the first stage are: 120℃ and 3.0MPa for 15min extraction.

[0037] Preferably, in step (3), the conditions of the second stage are: 140-160℃ and 4.5-5.5MPa for 5-10 min extraction.

[0038] More preferably, in step (3), the conditions of the second stage are: 150℃ and 5MPa pressure for 8 minutes of extraction.

[0039] Preferably, in step (4), the merging process involves vacuum concentration before or after merging, followed by freeze-drying to obtain pear cactus extract.

[0040] More preferably, in step (4), the purpose of the vacuum concentration treatment is to remove most of the water, which is beneficial for subsequent drying. In some specific embodiments of the present invention, the vacuum concentration is concentrated to 1.20-1.25 (25°C).

[0041] Then, the present invention provides a method for preparing the above composition, comprising the steps of: mixing tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying to obtain the composition.

[0042] Preferably, the freeze-drying time is 12-36 hours; more preferably, the freeze-drying time is 24 hours.

[0043] Finally, the present invention provides the use of the above composition in the preparation of food products with improved skin hydration and antioxidant properties.

[0044] Preferably, the food used in the application is not limited to health food, special dietary food, or ordinary food.

[0045] Preferably, the food is not limited to liquid beverage preparations, solid beverages, capsules, or granules.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] 1. In the composition of the present invention, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, sacha inchi oil, and wolfberry pulp work synergistically to deliver unexpected synergistic effects, enhancing water retention, skin elasticity, and antioxidant properties. The composition of the present invention can improve the inhibition rate of DPPH free radicals, achieving an inhibition rate of over 90% at relatively low concentrations. Furthermore, in human trials, after 30 days of use and testing, significant improvements were observed in skin hydration and elasticity; the increases in skin moisture content and elasticity were significantly higher than in the comparative example.

[0048] 2. In the composition of the present invention, the prickly pear cactus extract is obtained by juicing the raw material, treating it with Lactobacillus acidophilus, lactic acid bacteria and cellulase, and then performing subcritical water extraction, which significantly improves the yield of the prickly pear cactus extract. In the composition of the present invention, the prickly pear cactus extract, sodium hyaluronate, collagen tripeptide, Sacha inchi oil and wolfberry pulp work synergistically to bring about significant effects of water retention, moisturizing, increasing skin elasticity and anti-oxidation. The prickly pear cactus extract prepared by the preparation method of the present invention has a high content of active ingredients, resulting in excellent water retention and water-locking effects. Detailed Implementation

[0049] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0050] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0051] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0052] In a specific embodiment of the present invention, purchase information for some raw material components is shown in Table 1. Products from different manufacturers do not have a significant impact on the efficacy.

[0053] In the following specific embodiments of the present invention, the pear cactus raw material is a fresh raw material, and its water content is 92% after testing.

[0054] Table 1

[0055]

[0056] Example 1

[0057] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 50 parts Tremella fuciformis polysaccharide, 60 parts sodium hyaluronate, 30 parts collagen tripeptide, 30 parts prickly pear cactus extract, 10 parts black chokeberry powder, 10 parts sacha inchi oil, 20 parts wolfberry puree and 2 parts vitamin C.

[0058] The preparation method of the pear cactus extract is as follows:

[0059] (1) Pear cactus was mixed with water, and the solid-liquid ratio was 1g:25mL. Juice was extracted at 400W for 5min, and then filtered to obtain filtrate A and residue A.

[0060] (2) Filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:15mL (the mass of the solid is based on the pear cactus in step (1)); it is treated with Lactobacillus acidophilus, lactic acid bacteria and cellulase at 36℃ for 24h, filtered, and filtrate B and filter residue B are obtained.

[0061] The mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:3, and the total amount of the two bacteria is 8% of the mass of the prickly pear cactus; the amount of cellulase is 100 U / g, and the substrate is prickly pear cactus.

[0062] (3) Filter residue B was extracted in stages using a subcritical water extraction system with water as the solvent at a flow rate of 3 mL / min:

[0063] First stage: Extract at 120℃ and 3MPa for 15min, filter, and obtain filtrate C and filter residue C;

[0064] Second stage: Extraction at 150℃ and 5MPa for 8 minutes, followed by filtration to obtain filtrate D and filter residue D.

[0065] (4) Combine filtrate A, filtrate B, filtrate C and filtrate D, concentrate under reduced pressure to 1.20-1.25 (25℃), freeze dry for 24 h to obtain pear cactus extract.

[0066] The composition is prepared by mixing Tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0067] Example 2

[0068] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 40 parts Tremella fuciformis polysaccharide, 50 parts sodium hyaluronate, 35 parts collagen tripeptide, 35 parts prickly pear cactus extract, 12 parts black chokeberry powder, 8 parts sacha inchi oil, 25 parts wolfberry puree, and 1.5 parts vitamin C.

[0069] The preparation method of the pear cactus extract is as follows:

[0070] (1) Pear cactus was mixed with water, and the solid-liquid ratio was 1g:20mL. Juice was extracted at 300W for 10min, and then filtered to obtain filtrate A and residue A.

[0071] (2) Filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:20mL (the mass of the solid is based on the pear cactus in step (1)); it is treated with Lactobacillus acidophilus, lactic acid bacteria and cellulase at 40℃ for 12h, filtered, and filtrate B and filter residue B are obtained.

[0072] The mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:2, and the total amount of the two bacteria is 5% of the mass of the prickly pear cactus; the amount of cellulase is 150 U / g, and the substrate is prickly pear cactus.

[0073] (3) Filter residue B was extracted in stages using a subcritical water extraction system with water as the solvent at a flow rate of 2 mL / min:

[0074] First stage: Extract at 110℃ and 2MPa for 20min, then filter to obtain filtrate C and filter residue C;

[0075] Second stage: Extract at 160℃ and 5.5MPa for 5 minutes, then filter to obtain filtrate D and filter residue D.

[0076] (4) Combine filtrate A, filtrate B, filtrate C and filtrate D, concentrate under reduced pressure to 1.20-1.25 (25℃), freeze dry for 24 h to obtain pear cactus extract.

[0077] The composition is prepared by mixing Tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0078] Example 3

[0079] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 60 parts Tremella fuciformis polysaccharide, 70 parts sodium hyaluronate, 25 parts collagen tripeptide, 25 parts prickly pear cactus extract, 8 parts black chokeberry powder, 12 parts sacha inchi oil, 15 parts wolfberry puree, and 2.5 parts vitamin C.

[0080] The preparation method of the pear cactus extract is as follows:

[0081] (1) Pear cactus was mixed with water, and the solid-liquid ratio was 1g:30mL. Juice was extracted at 500W for 3min, and then filtered to obtain filtrate A and residue A.

[0082] (2) Filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:10mL (the mass of the solid is based on the pear cactus in step (1)); it is treated with Lactobacillus acidophilus, lactic acid bacteria and cellulase at 34℃ for 48h, filtered, and filtrate B and filter residue B are obtained.

[0083] The mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:4, and the total amount of the two bacteria is 10% of the mass of the prickly pear cactus; the amount of cellulase is 100 U / g, and the substrate is prickly pear cactus.

[0084] (3) Filter residue B was extracted in stages using a subcritical water extraction system with water as the solvent at a flow rate of 4 mL / min:

[0085] First stage: Extract at 130℃ and 4MPa for 10 min, then filter to obtain filtrate C and filter residue C;

[0086] Second stage: Extraction at 140℃ and 4.5MPa for 10 min, followed by filtration to obtain filtrate D and filter residue D.

[0087] (4) Combine filtrate A, filtrate B, filtrate C and filtrate D, concentrate under reduced pressure to 1.20-1.25 (25℃), freeze dry for 24 h to obtain pear cactus extract.

[0088] The composition is prepared by mixing Tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0089] Example 4

[0090] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 35 parts Tremella fuciformis polysaccharide, 40 parts sodium hyaluronate, 40 parts collagen tripeptide, 40 parts prickly pear cactus extract, 15 parts black chokeberry powder, 5 parts sacha inchi oil, 30 parts wolfberry puree, and 1 part vitamin C.

[0091] Everything else is the same as in Example 1.

[0092] Example 5

[0093] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 75 parts Tremella fuciformis polysaccharide, 80 parts sodium hyaluronate, 20 parts collagen tripeptide, 20 parts prickly pear cactus extract, 5 parts black chokeberry powder, 15 parts sacha inchi oil, 10 parts wolfberry puree, and 3 parts vitamin C.

[0094] Everything else is the same as in Example 1.

[0095] Comparative Example 1

[0096] Unlike Example 1, the pear cactus extract was replaced with pomegranate concentrate.

[0097] The composition is prepared by mixing tremella polysaccharide, sodium hyaluronate, collagen tripeptide, pomegranate concentrate, black chokeberry powder, sacha inchi oil, wolfberry juice and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0098] Comparative Example 2

[0099] Unlike Example 1, sacha inchi oil was replaced with high-oleic sunflower seed oil.

[0100] The composition is prepared by mixing tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, high oleic sunflower seed oil, wolfberry pulp and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0101] Comparative Example 3

[0102] Unlike Example 1, collagen tripeptide was replaced with salmon nasal cartilage powder.

[0103] The composition is prepared by mixing Tremella polysaccharide, sodium hyaluronate, salmon nasal cartilage powder, prickly pear cactus extract, black choke powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0104] Comparative Example 4

[0105] Unlike Example 1, the composition has different components.

[0106] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 50 parts Tremella fuciformis polysaccharide, 60 parts sodium hyaluronate, 30 parts collagen tripeptide, 20 parts wolfberry juice, and 2 parts vitamin C.

[0107] The composition is prepared by mixing Tremella polysaccharide, sodium hyaluronate, collagen tripeptide, wolfberry juice and vitamin C evenly, and freeze-drying for 24 hours to obtain the composition.

[0108] Comparative Example 5

[0109] Unlike Example 1, the composition has different components.

[0110] A composition for improving skin hydration and providing antioxidant benefits, comprising, by weight, 30 parts prickly pear cactus extract, 10 parts black chokeberry powder, and 10 parts sacha inchi oil.

[0111] The composition is prepared by mixing pear cactus extract, black chokeberry powder, and sacha inchi oil evenly and freeze-drying for 24 hours to obtain the composition.

[0112] Comparative Example 6

[0113] Unlike Example 1, the weight ratio of each component in the composition is different, specifically:

[0114] A composition for improving skin hydration and providing antioxidant effects, comprising, by weight, the following components: 15 parts Tremella fuciformis polysaccharide, 50 parts sodium hyaluronate, 50 parts collagen tripeptide, 30 parts prickly pear cactus extract, 27 parts black chokeberry powder, 27 parts sacha inchi oil, 11 parts wolfberry puree, and 2 parts vitamin C.

[0115] Everything else is the same as in Example 1.

[0116] Comparative Example 7

[0117] Unlike Example 1, the preparation method of the pear cactus extract is different. Specifically, the subcritical water extraction in step (3) is a one-step subcritical water extraction.

[0118] The preparation method of pear cactus extract is as follows:

[0119] Steps (1)-(2) are the same as in Example 1;

[0120] Step (3): The filter residue B is extracted using a subcritical water extraction system with water as the solvent at a flow rate of 3 mL / min. The extraction is carried out at 150℃ and 5 MPa for 23 min, followed by filtration to obtain filtrate D and filter residue D.

[0121] (4) Combine filtrate A, filtrate B, filtrate C and filtrate D, concentrate under reduced pressure to 1.20-1.25 (25℃), freeze dry for 24 h to obtain pear cactus extract.

[0122] Everything else is the same as in Example 1.

[0123] Comparative Example 8

[0124] Unlike Example 1, the preparation method of the pear cactus extract is different, specifically: Lactobacillus acidophilus and lactic acid bacteria are replaced with pectinase.

[0125] The preparation method of pear cactus extract is as follows:

[0126] (1) Pear cactus was mixed with water, and the solid-liquid ratio was 1g:25mL. Juice was extracted at 400W for 5min, and then filtered to obtain filtrate A and residue A.

[0127] (2) Filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:10-20mL (the mass of the solid is based on the pear cactus in step (1)); pectinase and cellulase are used to treat the mixture at 36℃ for 24h, and then filtered to obtain filtrate B and filter residue B.

[0128] The amount of pectinase used is 100 U / g, and the substrate is prickly pear cactus; the amount of cellulase used is 100 U / g, and the substrate is prickly pear cactus.

[0129] Steps (3)-(4) are the same as in Example 1.

[0130] Everything else is the same as in Example 1.

[0131] Experiment 1: Yield of Pyrus pyrifolia extract

[0132] The yields of the pear cactus extracts prepared in Examples 1-3 and Comparative Examples 7-8 were calculated. Five parallel experiments were conducted, and the results are shown in Table 2.

[0133] The yield (%) of prickly pear cactus extract = mass of prickly pear cactus extract / mass of prickly pear cactus (dry basis) × 100%. Explanation: Take 10g of prickly pear cactus, calculate its dry basis mass based on a water content of 92% as 0.8g, and obtain 0.32g of prickly pear cactus extract. Therefore, the mass yield of prickly pear cactus extract is 40%.

[0134] Table 2

[0135]

[0136] As can be seen from Table 2, the preparation method of the pear cactus extract of the present invention can significantly improve the yield of the pear cactus extract, resulting in a significant increase in yield.

[0137] Experiment 2: DPPH free radical scavenging rate experiment

[0138] Take equal masses of the compositions prepared in Examples 1-5 and the compositions prepared in Comparative Examples 1-8, and mix them with deionized water to prepare sample solutions with a concentration of 10 mg / mL; dissolve DPPH in anhydrous ethanol to prepare a 0.2 mmol / L DPHH solution.

[0139] Mix 2 mL of DPHH solution with 2 mL of anhydrous ethanol to prepare a mixture, denoted as C0; mix 2 mL of sample solution with 2 mL of anhydrous ethanol to prepare a mixture, denoted as C1; mix 2 mL of sample solution with 2 mL of DPHH solution to prepare a mixture, denoted as C2. DPPH free radical scavenging rate = (1 - (C1 - C2) / C0) × 100%. Record the data and repeat the experiment 3 times in parallel. The average value and standard deviation of DPPH free radical scavenging rate are shown in Table 3.

[0140] Table 3

[0141]

[0142] In Table 3, ##This indicates that the DPPH radical scavenging rate was significantly different between the comparative group and the Example 1 group. ## This indicates that P < 0.01.

[0143] As can be seen from Table 3, the prickly pear cactus extract prepared by a specific method in the composition of the present invention works synergistically with the other components in the composition to achieve a DPPH free radical scavenging rate of over 90%. Compared with the comparative example group, the composition of the present invention has better antioxidant efficacy. The comparison of the technical effects of the examples, comparative example 4, and comparative example 5 shows that the components in the composition of the present invention work synergistically, resulting in superior antioxidant performance compared to the sum of the effects when the components are used separately.

[0144] Experiment 3: Human Experiment

[0145] 1. The subjects were women aged 30-50 with dry and loose skin.

[0146] Exclusion criteria for participants:

[0147] (1) Pregnant or lactating women, those with allergies, and those allergic to this health food.

[0148] (2) Those with a history of cardiovascular, cerebrovascular, liver, kidney, hematopoietic system diseases and mental illness.

[0149] (3) Taking items related to the test function in a short period of time may affect the judge of the results.

[0150] (4) Those who did not take the test sample as required by the test, whose efficacy could not be determined or whose efficacy or safety was not determined due to incomplete data.

[0151] 2. Grouping

[0152] The participants were randomly divided into 13 groups based on their age, with 8 participants in each group. All 13 groups consumed a basic diet plus 2g of the composition daily (experimental group).

[0153] Measurement environment: The measurement was conducted in a spacious, well-ventilated testing room with stable temperature and humidity. The test area was cleaned with a clean cotton ball dipped in distilled water in a quiet environment, dried, and the moisture content was measured 15 minutes later. The same instrument and the same person performed the measurements before and after the food tasting.

[0154] 3. Testing

[0155] The moisture content and elasticity of the subjects' facial skin (forehead) were tested using a skin tester before consumption and 30 days after consumption, and the average value of each group was taken.

[0156] Skin moisture content was tested using the RealBubeeRBX-916 skin stratum corneum moisture analyzer. Skin moisture content before use was recorded as W0, and skin moisture content after 30 days of use was recorded as W30. The increase in skin moisture content was calculated as follows:

[0157] Increase in skin moisture content (%) = (W30-W0) / W0×100%.

[0158] Skin elasticity R2 value was tested using a Cutometer (Courage & Khazaka, Germany); the higher the value (closer to 1), the better the skin elasticity. The skin elasticity R2 value before use was recorded as T0, and the skin elasticity R2 value after 30 days of use was recorded as T30. The increase in skin elasticity was calculated as follows:

[0159] Increase in skin elasticity (%) = (T30-T0) / T0×100%.

[0160] The results of the increase in skin moisture content and the increase in skin elasticity are shown in Table 4.

[0161] Table 4

[0162]

[0163] In Table 4, # and ## This indicates that the data in the comparative example group are significantly different from those in Example 1 group. # This indicates that P < 0.05. ## This indicates that P < 0.01.

[0164] As can be seen from Table 4, the composition of the present invention can significantly improve skin moisture content and skin elasticity, and has excellent effects in moisturizing and locking in moisture, improving skin hydration, and enhancing skin elasticity.

[0165] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for improving skin hydration and providing antioxidant effects, characterized in that, By weight, it consists of the following components: 35-75 parts Tremella polysaccharide, 40-80 parts sodium hyaluronate, 20-40 parts collagen tripeptide, 20-40 parts pear cactus extract, 5-15 parts black chokeberry powder, 5-15 parts sacha inchi oil, 10-30 parts wolfberry puree and 1-3 parts vitamin C; The preparation method of the pear cactus extract is as follows: (1) Mix pear cactus with water, juice it, filter it, and obtain filtrate A and residue A; (2) Filter residue A is mixed with water and treated with Lactobacillus acidophilus, lactic acid bacteria and cellulase, and then filtered to obtain filtrate B and filter residue B; (3) Filter residue B is extracted in stages using a subcritical water extraction system with water as the solvent: First stage: Extraction at 110-130℃ and pressure 2.0-4.0MPa, followed by filtration to obtain filtrate C and filter residue C; Second stage: Extraction at 140-160℃ and pressure 4.5-5.5MPa, followed by filtration to obtain filtrate D and filter residue D; (4) Combine filtrate A, filtrate B, filtrate C and filtrate D to obtain pear cactus extract.

2. The composition according to claim 1, characterized in that, By weight, it consists of the following components: 40-60 parts Tremella polysaccharide, 50-70 parts sodium hyaluronate, 25-35 parts collagen tripeptide, 25-35 parts pear cactus extract, 8-12 parts black chokeberry powder, 8-12 parts sacha inchi oil, 15-25 parts wolfberry puree, and 1.5-2.5 parts vitamin C.

3. The composition according to claim 2, characterized in that, By weight, it consists of the following components: 50 parts Tremella polysaccharide, 60 parts sodium hyaluronate, 30 parts collagen tripeptide, 30 parts pear cactus extract, 10 parts black chokeberry powder, 10 parts sacha inchi oil, 20 parts wolfberry puree and 2 parts vitamin C.

4. The composition according to any one of claims 1-3, characterized in that, In step (1), the solid-liquid ratio of pear cactus and water is 1g:20-30mL; in step (1), the juicing process is carried out at 300-500W for 3-10 minutes.

5. The composition according to any one of claims 1-3, characterized in that, In step (2), the filter residue A is mixed with water, and the solid-liquid ratio of the mixture is 1g:10-20mL, wherein the mass of the solid is based on the pear cactus in step (1); In step (2), the mass ratio of Lactobacillus acidophilus to lactic acid bacteria is 1:2-4.

6. The composition according to any one of claims 1-3, characterized in that, In step (2), the total amount of Lactobacillus acidophilus and lactic acid bacteria used is 5%-10% of the mass of the prickly pear cactus; In step (2), the amount of cellulase used is 50-150 U / g; in step (2), the treatment conditions are: treatment at 34-40℃ for 12-48h.

7. The composition according to any one of claims 1-3, characterized in that, In step (3), the water flow rate during the staged extraction is 2-4 mL / min.

8. The composition according to any one of claims 1-3, characterized in that, In step (3), the specific conditions for the first stage are: extraction at 110-130℃ and 2.0-4.0MPa for 10-20 minutes; In step (3), the specific conditions for the second stage are: 140-160℃ and 4.5-5.5MPa for 5-10 min extraction.

9. A method for preparing the composition according to any one of claims 1-8, comprising the steps of: mixing tremella polysaccharide, sodium hyaluronate, collagen tripeptide, prickly pear cactus extract, black chokeberry powder, sacha inchi oil, wolfberry pulp and vitamin C evenly, and freeze-drying to obtain the composition.

10. The use of the composition according to any one of claims 1-8 in the preparation of food products having improved skin hydration and antioxidant properties.

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