Anti-hair loss composition and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and relates to an anti-hair loss composition and application thereof, the composition comprises a cacumen biotae extract, a peppermint leaf extract, a pomegranate peel extract, polypeptide, biotin and amino acid. The composition disclosed by the invention is not simply piled up of the components, but based on deep understanding and scientific compatibility of the effects of the components, the synergistic effect of the components is verified through cell experiments, the multi-dimensional and multi-channel comprehensive alopecia prevention and treatment effect is realized, the potential side effects of hormones, minoxidil and other medicines are avoided, and the composition is mild, safe and free of toxic and side effects. And the product is suitable for long-term use of wider people.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to an anti-hair loss composition and its application. Background Technology

[0002] Hair loss, especially androgenetic alopecia (also known as seborrheic alopecia or premature baldness), is a common hair disease with a high incidence rate and a trend towards affecting younger people. It has a serious negative impact on patients' appearance, mental health, and quality of life. The causes of hair loss are complex and are closely related to multiple mechanisms, including genetic factors, androgen metabolism, microcirculatory disorders in hair follicles, inflammation around hair follicles, oxidative stress, and imbalance of growth factors.

[0003] Currently, there are two main technological approaches in the field of hair loss prevention products: one is based on formulas containing natural plant extracts, which have a gentle effect and outstanding advantages in systemic conditioning, but often have a slow onset of action, insufficient targeting, and limited direct activation ability of hair follicles; the other is based on formulas containing chemically synthesized active peptides, which have strong targeting and relatively fast results, but have a single mechanism of action, lack overall improvement of the scalp microecology, and the safety and stability of long-term use are questionable. Neither single technological approach can simultaneously meet the dual requirements of "fast and efficient" and "systemic safety." Therefore, existing technologies lack a hair loss prevention and hair regrowth solution that can deeply integrate the systemic conditioning of natural plants with the precise targeting of modern active ingredients to produce a significant synergistic effect. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a hair loss prevention composition and its application.

[0005] To achieve this objective, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides an anti-hair loss composition comprising arborvitae leaf extract, peppermint leaf extract, pomegranate peel extract, polypeptides, biotin, and amino acids.

[0007] The primary objective of this invention is to overcome the shortcomings of existing technologies and provide a novel active composition for preventing hair loss and promoting hair growth. This composition combines specifically formulated natural plant extracts with precisely proportioned bioactive ingredients (biotin, tripeptide-1, acetyl tetrapeptide-3, and sarcosine), leveraging the multi-target and systemic conditioning effects of plant extracts on the scalp environment (such as anti-inflammatory, antibacterial, and improved microcirculation) and the precise promotion of hair follicle cell proliferation and repair by bioactive ingredients. This provides a safe, highly effective, and clearly defined mechanism of action combination suitable for preventing and improving various types of hair loss in cosmetics or skincare products.

[0008] This invention combines network pharmacology with the TCMSP / BATMAN / HERB / ETCM traditional Chinese medicine database and analysis platform to screen and summarize the potential targets of active and bioactive components of the plant extracts proposed in this invention. It also retrieves targets related to hair loss from openTargets / OMIM / GeneCards databases, constructs a Venn diagram of compound targets and hair loss diseases, and obtains the intersection target list between drug targets and disease targets. Pathway and process enrichment analyses are performed on the intersection targets: KEGG pathway, GO biological process, response genome set, canonical pathway, CORUM, WikiPathways, and PANTHER pathway. Items with p-values ​​< 0.01, minimum counts of 3, and enrichment factors > 1.5 (enrichment factor is the ratio between observed counts and incidentally expected counts) are collected and grouped into clusters based on similarity to identify significant signaling pathways.

[0009] Preferably, the composition comprises, by weight parts, 0.01-10 parts of Platycladus orientalis leaf extract, 0.01-5 parts of peppermint leaf extract, 0.001-2 parts of pomegranate peel extract, 0.002-3 parts of polypeptide, 0.001-1 parts of biotin, and 0.001-5 parts of amino acids.

[0010] The mass fractions of Platycladus orientalis leaf extract can be selected from 0.01 parts, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, and 10 parts, etc. The mass fractions of Mentha haplocalyx leaf extract can be selected from 0.01 parts, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, and 5 parts, etc. The mass fractions of durian peel extract can be selected from 0.001 parts, 0.002 parts, 0.005 parts, 0.008 parts, 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, and 2 parts, etc., while the mass fractions of polypeptides can be selected from 0.002 parts, 0.005 parts, 0.008 parts, 0.01 parts, 0.0... Available in quantities of 5, 0.08, 0.1, 0.2, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, and 3 parts. The biotin mass can be selected in quantities of 0.001, 0.002, 0.005, 0.008, 0.01, 0.05, 0.08, 0.1, 0.2, 0.5, and 0. 8 parts, 1 part, and the mass fractions of amino acids are 0.001 parts, 0.002 parts, 0.005 parts, 0.008 parts, 0.01 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0011] Preferably, the polypeptide comprises tripeptide-1 and acetyl tetrapeptide-3.

[0012] Preferably, the mass ratio of tripeptide-1 to acetyl tetrapeptide-3 is (0.001-1):(0.001-2).

[0013] The specific point values ​​in (0.001-1) can all be selected from 0.001, 0.002, 0.005, 0.008, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1, etc. The specific point values ​​in (0.001-2) can all be selected from 0.001, 0.002, 0.005, 0.008, 0.01, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, etc. Other specific point values ​​within the above range can also be selected, which will not be elaborated here.

[0014] Preferably, the amino acid is sarcosine.

[0015] Preferably, the pomegranate peel extract is prepared by a method comprising the following steps:

[0016] (1) Pomegranate peel was mixed with an ethanol aqueous solution and subjected to microwave-ultrasound combined treatment to obtain the first mixture;

[0017] (2) The first mixture was subjected to negative pressure cavitation extraction to obtain an extract;

[0018] (3) Refrigerate the extract to coagulate, collect the supernatant, and dry it to obtain the final product.

[0019] Preferably, the ratio of pomegranate peel to ethanol aqueous solution is 1 g:(20-30) mL. The specific values ​​in (20-30) can be selected from 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0020] Preferably, the ethanol aqueous solution contains 40-60% ethanol by mass, such as 40%, 42%, 44%, 46%, 48%, 50%, 52%, 54%, 56%, 58%, 60%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0021] Preferably, the temperature of the combined treatment is 40-80°C.

[0022] Temperatures can be selected from 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃, etc. Other specific values ​​within the above range can also be selected, which will not be elaborated here.

[0023] Preferably, the ultrasonic treatment has a power of 50-400 W and a duration of 3-10 min.

[0024] The power can be selected from 50 W, 100 W, 150 W, 200 W, 250 W, 300 W, 350 W, 400 W, etc., and the time can be selected from 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0025] Preferably, the microwave treatment power is 200-500 W, and the microwave treatment time is 3-15 min.

[0026] The power can be selected from 200 W, 250 W, 300 W, 350 W, 400 W, 450 W, 500 W, etc., and the time can be selected from 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 11 min, 12 min, 13 min, 14 min, 15 min, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0027] Preferably, the negative pressure cavitation extraction temperature is 40-70℃, the time is 20-40 min, and the vacuum degree is 60-120 mbar.

[0028] Temperatures can be selected from 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, etc.; time can be selected from 20 min, 25 min, 30 min, 35 min, 40 min, etc.; vacuum levels can be selected from 60 mbar, 65 mbar, 70 mbar, 75 mbar, 80 mbar, 85 mbar, 90 mbar, 95 mbar, 100 mbar, 105 mbar, 110 mbar, 115 mbar, 120 mbar, etc. Other specific values ​​within the above ranges can also be selected, which will not be elaborated here.

[0029] Preferably, the refrigerated flocculation temperature is 2-8℃ and the time is 10-30 h.

[0030] Temperatures can be selected from 2℃, 3℃, 4℃, 5℃, 6℃, 7℃, 8℃, etc., and time can be selected from 10 h, 12 h, 15 h, 18 h, 20 h, 22 h, 25 h, 28 h, 30 h, etc. Other specific point values ​​within the above range can be selected, which will not be elaborated here.

[0031] In a second aspect, the present invention provides the use of the anti-hair loss composition according to the first aspect in the preparation of a product having anti-hair loss efficacy.

[0032] Preferably, the product includes cosmetics, skin care products, or pharmaceuticals.

[0033] Preferably, the hair loss prevention composition has a mass percentage content of 12-16% in the product, such as 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] (1) Network pharmacology analysis results show that the bioactive components in this invention mainly act on core cell signaling pathways such as Ras and the epithelial cell differentiation process. When combined with plant extracts, they produce a significant synergistic effect, adding regulation of core hair follicle regeneration pathways such as Hippo and VEGF, and greatly enhancing the improvement effect on the immune microenvironment and blood circulation system. The mechanism of action of this combination scheme achieves full coverage from "precise cell regulation" to "system environment improvement", and its effect is far superior to that of a single component, demonstrating outstanding substantive characteristics and significant progress.

[0036] (2) Cell experiments verified the network pharmacology analysis results, demonstrating a synergistic effect of "1+1>2". Experimental data fully demonstrate that the composition exhibits significantly higher proliferative activity of human dermal papilla cells than the plant extract group and the bioactive ingredient group, showcasing unexpectedly superior effects. The composition is not a simple accumulation of ingredients, but rather based on a deep understanding of the efficacy of each ingredient and scientific compatibility. Its synergistic effect has been verified through cell experiments, and its mechanism of action is clear and well-defined, breaking through the limitations of traditional Chinese medicine compound prescriptions that are "based on experience".

[0037] (3) The composition prepared by this invention has both "systemic conditioning" and "precise activation" mechanisms. The plant components create a healthy scalp microenvironment for hair follicle growth (treating the root cause), while the bioactive components directly activate key signaling pathways of hair follicle cells (treating the symptoms). This achieves a comprehensive prevention and treatment effect in multiple dimensions and pathways, avoiding the potential side effects of hormones, minoxidil and other drugs. It is mild and safe and suitable for long-term use by a wider range of people. Attached Figure Description

[0038] Figure 1 It is the result of the intersection of target points.

[0039] Figure 2 This is the result of pathway and process enrichment analysis of the intersection targets of plant extracts.

[0040] Figure 3 It is the result of pathway and process enrichment analysis of the intersection targets of bioactive components.

[0041] Figure 4 It is the result of pathway and process enrichment analysis of plant extracts and bioactive components. Detailed Implementation

[0042] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0043] The peppermint leaf extract was purchased from Shanghai Jiyan Biomedical Development Co., Ltd., and its product is called peppermint leaf extract.

[0044] The Platycladus orientalis leaf extract was purchased from Shanghai Jiyan Biomedical Development Co., Ltd., under the product name Platycladus orientalis leaf extract.

[0045] Preparation Example 1

[0046] This preparation example provides a method for preparing pomegranate peel extract, the method comprising:

[0047] (1) After crushing the pomegranate peel, it was mixed with an ethanol aqueous solution with a mass percentage of 50% at a material-liquid ratio of 1 g: 25 mL and subjected to microwave-ultrasound combined treatment. The microwave treatment power was 300 W and the time was 8 min; the ultrasonic treatment power was 200 W and the time was 5 min. The temperature of the combined treatment was 60℃ to obtain the first mixture.

[0048] (2) The first mixture was subjected to negative pressure cavitation extraction. The vacuum degree of negative pressure cavitation was 100 mbar, the temperature was 50℃, and the time was 30 min to obtain the extract.

[0049] (3) The extract was refrigerated at 4°C for 20 h to flocculate, the supernatant was collected by filtration and dried to obtain the final product.

[0050] Preparation Example 2

[0051] This preparation example provides a method for preparing pomegranate peel extract, the method comprising:

[0052] (1) After crushing the pomegranate peel, it was mixed with an ethanol aqueous solution with a mass percentage of 40% at a material-liquid ratio of 1 g: 20 mL and subjected to microwave-ultrasound combined treatment. The microwave treatment power was 450 W and the time was 3 min; the ultrasonic treatment power was 100 W and the time was 8 min. The temperature of the combined treatment was 40℃ to obtain the first mixture.

[0053] (2) The first mixture was subjected to negative pressure cavitation extraction. The vacuum degree of negative pressure cavitation was 120 mbar, the temperature was 70℃, and the time was 20 min to obtain the extract.

[0054] (3) The extract was refrigerated at 2°C for 10 h to flocculate, the supernatant was collected by filtration and dried to obtain the final product.

[0055] Preparation Example 3

[0056] This preparation example provides a method for preparing pomegranate peel extract, the method comprising:

[0057] (1) After crushing the pomegranate peel, it was mixed with an ethanol aqueous solution with a mass percentage of 60% at a material-liquid ratio of 1 g:30 mL and subjected to microwave-ultrasound combined treatment. The microwave treatment power was 200 W and the time was 12 min; the ultrasonic treatment power was 400 W and the time was 3 min. The temperature of the combined treatment was 80℃ to obtain the first mixture.

[0058] (2) The first mixture was subjected to negative pressure cavitation extraction. The vacuum degree of negative pressure cavitation was 60 mbar, the temperature was 40℃, and the time was 40 min to obtain the extract.

[0059] (3) The extract was refrigerated at 8°C for 30 h to coagulate, the supernatant was collected by filtration and dried to obtain the final product.

[0060] Preparation Example 4

[0061] This preparation example provides a method for preparing pomegranate peel extract. The only difference between this method and Preparation Example 1 is that step (1) is to "crush the pomegranate peel and mix it with an ethanol aqueous solution with a mass percentage of 50% at a material-to-liquid ratio of 1 g:25 mL, and then perform microwave treatment. The microwave treatment power is 300 W, the time is 15 min, and the treatment temperature is 60°C to obtain the first mixture". Other operations remain unchanged.

[0062] Preparation Example 5

[0063] This preparation example provides a method for preparing pomegranate peel extract. The only difference between this method and Preparation Example 1 is that step (1) is to "crush the pomegranate peel and mix it with an ethanol aqueous solution with a mass percentage of 50% at a material-liquid ratio of 1 g:25 mL, and then perform ultrasonic treatment. The ultrasonic treatment power is 300 W, the time is 10 min, and the treatment temperature is 60℃ to obtain the first mixture". Other operations remain unchanged.

[0064] Preparation Example 6

[0065] This preparation example provides a method for preparing pomegranate peel extract. The only difference between this method and Preparation Example 1 is that step (1) is to "crush the pomegranate peel and mix it with an ethanol aqueous solution with a mass percentage of 50% at a material-to-liquid ratio of 1 g:25 mL, mix at a temperature of 60°C for 13 min to obtain a first mixture", while other operations remain unchanged.

[0066] Preparation Example 7

[0067] This preparation example provides a method for preparing pomegranate peel extract, which differs from preparation example 1 only in that step (2) is "extracting the first mixture at 50°C for 30 min to obtain the extract", while other operations remain unchanged.

[0068] Preparation Example 8

[0069] This preparation example provides a method for preparing pomegranate peel extract, which differs from preparation example 1 only in that step (2) is "to reflux the first mixture for 30 min to obtain the extract", while other operations remain unchanged.

[0070] Preparation Example 9

[0071] This preparation example provides a method for preparing pomegranate peel extract, which differs from preparation example 1 only in that step (3) is "filtering the extract, collecting the supernatant, drying, and obtaining the extract", while other operations remain unchanged.

[0072] Example 1

[0073] This embodiment provides a hair loss prevention composition, which includes, by weight parts, 5 parts of Platycladus orientalis leaf extract, 3 parts of peppermint leaf extract, 1 part of pomegranate peel extract prepared in Preparation Example 1, 2 parts of polypeptide, 0.5 parts of biotin, and 3 parts of sarcosine. The polypeptide is tripeptide-1 and acetyl tetrapeptide-3 in a mass ratio of 1:1.

[0074] The preparation method is as follows: the raw materials are physically mixed to obtain the product.

[0075] Example 2

[0076] This embodiment provides a hair loss prevention composition, which includes, by mass parts, 1 part of Platycladus orientalis leaf extract, 5 parts of peppermint leaf extract, 2 parts of pomegranate peel extract prepared in Preparation Example 2, 3 parts of polypeptide, 0.1 parts of biotin, and 5 parts of sarcosine. The polypeptide is tripeptide-1 and acetyl tetrapeptide-3 in a mass ratio of 0.1:2.

[0077] The preparation method is the same as in Example 1.

[0078] Example 3

[0079] This embodiment provides a hair loss prevention composition, which includes, by mass parts, 10 parts of Platycladus orientalis leaf extract, 1 part of peppermint leaf extract, 0.1 parts of pomegranate peel extract prepared in Preparation Example 3, 0.1 parts of polypeptide, 1 part of biotin, and 0.1 parts of sarcosine. The polypeptide is tripeptide-1 and acetyl tetrapeptide-3 in a mass ratio of 1:0.1.

[0080] The preparation method is the same as in Example 1.

[0081] Examples 4-9

[0082] This embodiment provides six anti-hair loss compositions, which differ from Example 1 only in that the pomegranate peel extract prepared in Preparation Example 1 is replaced in equal amounts with the pomegranate peel extract prepared in Preparation Examples 4-9, while other components and contents remain unchanged.

[0083] The preparation method is the same as in Example 1.

[0084] Comparative Example 1

[0085] This comparative example provides a hair loss prevention composition that differs from Example 1 only in that it does not contain Platycladus orientalis leaf extract, and the reduced mass of Platycladus orientalis leaf extract is proportionally allocated to the mass of peppermint leaf extract and pomegranate peel extract prepared in Example 1, while other ingredients and contents remain unchanged.

[0086] The preparation method is the same as in Example 1.

[0087] Comparative Example 2

[0088] This comparative example provides a hair loss prevention composition that differs from Example 1 only in that it does not contain peppermint leaf extract, and the reduced mass of peppermint leaf extract is proportionally allocated to the mass of arborvitae leaf extract and pomegranate peel extract prepared in Preparation Example 1, while other ingredients and contents remain unchanged.

[0089] The preparation method is the same as in Example 1.

[0090] Comparative Example 3

[0091] This comparative example provides a hair loss prevention composition, which differs from Example 1 only in that it does not contain the pomegranate peel extract prepared in Example 1, and the reduced mass of the pomegranate peel extract is proportionally allocated to the mass of the arborvitae leaf extract and peppermint leaf extract, while the other ingredients and contents remain unchanged.

[0092] The preparation method is the same as in Example 1.

[0093] Comparative Example 4

[0094] This comparative example provides a hair loss prevention composition that differs from Example 1 only in that it does not contain biotin, and the reduced mass of biotin is proportionally allocated to the mass of sarcosine, tripeptide-1, and acetyl tetrapeptide-3, while the other ingredients and contents remain unchanged.

[0095] The preparation method is the same as in Example 1.

[0096] Comparative Example 5

[0097] This comparative example provides a hair loss prevention composition that differs from Example 1 only in that it does not contain sarcosine, and its reduced mass is proportionally allocated to the mass of biotin, tripeptide-1, and acetyl tetrapeptide-3, while other ingredients and contents remain unchanged.

[0098] The preparation method is the same as in Example 1.

[0099] Comparative Example 6

[0100] This comparative example provides a hair loss prevention composition that differs from Example 1 only in that it does not contain tripeptide-1 and acetyl tetrapeptide-3, and the reduced mass of these ingredients is proportionally allocated to the mass of biotin and sarcosine, while the other ingredients and their contents remain unchanged.

[0101] The preparation method is the same as in Example 1.

[0102] Comparative Example 7

[0103] This comparative example provides a hair loss prevention composition, which differs from Example 1 only in that it does not contain Platycladus orientalis leaf extract, peppermint leaf extract, or pomegranate peel extract prepared in Preparation Example 1. The reduced mass of these ingredients is proportionally allocated to the mass of tripeptide-1, acetyl tetrapeptide-3, biotin, and sarcosine.

[0104] The preparation method is the same as in Example 1.

[0105] Comparative Example 8

[0106] This comparative example provides a hair loss prevention composition, which differs from Example 1 only in that it does not contain tripeptide-1, acetyl tetrapeptide-3, biotin, or sarcosine, and the reduced mass of these ingredients is proportionally allocated to the mass of the arborvitae leaf extract, peppermint leaf extract, and pomegranate peel extract prepared in Preparation Example 1.

[0107] The preparation method is the same as in Example 1.

[0108] Test Example 1

[0109] The databases and their URLs used in this test case are shown in Table 1.

[0110] Table 1

[0111]

[0112] 1. The active ingredients and potential targets of the pomegranate peel extract, arborvitae leaf extract, and peppermint leaf extract of this invention were screened and summarized using the TCMSP / HERB / ETCM traditional Chinese medicine database and analysis platform. The results are shown in Table 2.

[0113] Table 2

[0114]

[0115] 2. Potential targets for biotin, tripeptide-1, acetyl tetrapeptide-3, and sarcosine were screened using database platforms such as SwissTargetPrediction, Pubchem, and PharmMapper, totaling 1118.

[0116] 3. Hair loss disease targets were retrieved using the keyword "Alopecia" from the "OpenTargets Score>=0.1 / OMIM / GeneCards Score>=5" database. Results were filtered according to thresholds, and the gene names of the standardized disease targets were summarized. After deduplication and intersection analysis, 882 disease targets were obtained. These targets were then mapped to plant extracts, bioactive components, and synergistic combinations of plant extracts and bioactive components. After mapping, the three experimental groups had 30, 22, and 52 overlapping targets, respectively. Figure 1 As shown.

[0117] Pathway and process enrichment analyses were performed on the intersection targets of the three experimental groups: KEGG pathway, GO biological process, response genome set, canonical pathway, CORUM, WikiPathways, and PANTHER pathway. Items with p-values ​​<0.01, minimum counts of 3, and enrichment factors >1.5 (enrichment factor is the ratio between observed counts and incidentally expected counts) were collected and grouped into clusters based on similarity to identify significant signaling pathways.

[0118] Results analysis:

[0119] (1) Characteristics of the effects of plant extracts:

[0120] Figure 2 The pathways shown indicate that it is primarily involved in a number of fundamental and broad biological processes:

[0121] G0:0002521: leukocyte differentiation: regulates immune cell function and may improve scalp micro-inflammation.

[0122] G0:0045596: Negative regulation of cell differentiation: This is a broad regulatory process, and its significance needs to be considered in the context of the specific situation.

[0123] G0:0008544: Epidermal Development: This is the most direct pathway related to hair loss prevention, promoting the normal development and health of the skin / hair follicle epidermis.

[0124] Lipid homeostasis / hormonal response: Regulates homeostasis to create a stable internal environment for hair growth.

[0125] In summary, plant extracts alone mainly focus on overall regulation (immunity, homeostasis) and basic development, with relatively broad but not precise effects.

[0126] (2) Characteristics of the action of bioactive components (alone):

[0127] 1) Regulation of core signaling pathways (precise intervention in cell behavior)

[0128] WP4223: Ras signaling pathway: Ras is a key intracellular signal transduction protein that regulates cell growth, division, survival, and differentiation. This pathway is a convergence point for many growth factor signals. Activation of the Ras pathway can directly promote the proliferation and vitality of dermal papilla cells and follicular keratinocytes, providing the most basic cellular driving force for hair growth.

[0129] M100: PID SHP2 PATHWAY & N91: PID TCPTP PATHWAY: SHP2 and TCPTP are both important tyrosine phosphatases that play the role of "regulators" in key signaling pathways such as JAK / STAT and MAPK, precisely controlling the intensity and duration of the signal.

[0130] 2) Hair follicle condition regulation

[0131] GO:0008285: Negative regulation of cell population proliferation:

[0132] In the hair follicle cycle, timely exit from the proliferative phase and entry into the differentiation phase is crucial for the formation of a healthy hair shaft. This pathway suggests that this peptide may be involved in maintaining the quiescent state of hair follicle stem cells (preventing excessive consumption) or inhibiting the fibrosis process around the hair follicle, creating a stress-free external environment for the hair follicle.

[0133] GO:0030855: Epithelial cell differentiation: The hair follicle is essentially an epithelial cell organ. The hair shaft is formed from differentiated epithelial cells (keratinocytes). Promoting epithelial cell differentiation means accelerating the formation and growth of the hair shaft, making the hair thicker and healthier.

[0134] WP2446: Retinoblastoma gene in cancer: The Rb gene is a well-known tumor suppressor gene and a core controller of the cell cycle G1 / S checkpoint. Regulating the Rb pathway means that it can directly control the process of hair follicle cells entering DNA replication and division (S phase) from quiescent (G1 phase), and is a key molecular switch that drives hair follicles from the resting phase to the growth phase.

[0135] 3) Development and morphogenesis (construction of hair follicle structure)

[0136] GO:0007423: Sensory organ development: The hair follicle itself is a tiny tactile sensory organ, with its base closely connected to nerve endings. This suggests that it may participate in the development and maintenance of the entire hair follicle organ, rather than simply stimulating cell proliferation.

[0137] GO:0061448: Connective tissue development: Connective tissue forms the dermal papilla of the hair follicle. Promoting connective tissue development means strengthening and activating the function of the dermal papilla, thereby sending a strong growth signal to the entire hair follicle.

[0138] hsa04360: Axon guidance: This pathway guides the growth of nerve axons. During development, the migration and signaling of nerve crest cells are closely related to hair follicle formation. This pathway may awaken the regeneration program of hair follicles by mimicking signals during development, promoting the generation of new hair follicles or the "regeneration" of existing hair follicles.

[0139] Summary: The results are as follows Figure 3 As shown, the mechanism of action of bioactive components differs from the "macro-systemic regulation" model of plant extracts. This combination operates on a "micro-level precise regulation" model: directly acting on core cellular signaling nodes such as Ras, Rb, and SHP2. Functions are highly correlated: the core functions revolve around the three most directly related aspects of hair follicle regeneration: "cell cycle regulation," "epithelial differentiation," and "structural development." The mechanism is modernly clear: its mechanism of action aligns with modern molecular and cell biology understanding of the hair follicle cycle, rather than traditional empirical medicine.

[0140] (3) Characteristics of the effects of plant extracts + bioactive ingredients (combination)

[0141] 1) Strongly promotes hair follicle cell proliferation and activation:

[0142] GO:0008283: Cell population proliferation: Directly promotes the proliferation of hair follicle cells (such as hair papilla cells and keratinocytes), which is the basis of hair growth.

[0143] GO:0071363: Cellular response to growth factor stimulus: Hair follicle growth is driven by various growth factors (such as VEGF, FGF, IGF). This pathway indicates that the composition can significantly enhance the cellular response to these key growth signals.

[0144] WP3888: VEGFA-VEGFR2 signaling (VEGF signaling pathway): This is one of the most important pathways in hair follicle regeneration. VEGF promotes angiogenesis around the hair follicle, providing sufficient nutrients and oxygen to the hair follicle, which is key to maintaining hair follicle health and productivity.

[0145] 2) Precisely regulate hair follicle cycle and development:

[0146] WP4540: Hippo signaling regulation: A core pathway for hair follicle regulation discovered in recent years. The Hippo pathway plays a decisive role in controlling the quiescence and activation of hair follicle stem cells, and regulating it means that the "anagen-telogen" phase transition of hair follicles can be directly intervened.

[0147] GO:0022612: Gland morphogenesis / GO:0060688: Regulation of morphogenesis of a branching structure: Hair follicles are essentially tiny branching glands. These pathways indicate that the composition promotes proper follicle formation and structural remodeling, which is crucial for follicles re-entering the anagen phase from the telogen phase.

[0148] 3) Deeply regulates the immune and inflammatory environment:

[0149] GO:0030098: lymphocyte differentiation: more refined regulation of the scalp immune environment may help suppress abnormal immune attacks against hair follicles (such as alopecia areata).

[0150] hsa05166: Human T-cell leukemia virus 1 infection: This pathway is rich in various cytokines and cellular signaling and is typically associated with strong immune and inflammatory responses. The composition's ability to act on this pathway suggests it possesses potent immunomodulatory and anti-inflammatory potential, which is crucial for inflammatory hair loss (such as the micro-inflammation associated with androgenetic alopecia).

[0151] 4) Improves scalp microcirculation and mechanical stimulation response:

[0152] GO:0071498: Cellular response to fluid shear stress: Hair follicle cells can sense the shear force from blood flow, and this mechanical stimulation itself can promote hair follicle growth. This pathway suggests that the composition may enhance the hair follicle's response to this benign stimulus, thereby promoting hair growth.

[0153] Summary: Figure 4 As shown, network pharmacology analysis results indicate that the bioactive components in this invention primarily act on core cell signaling pathways such as Ras and the epithelial cell differentiation process. When combined with plant extracts, they produce a significant synergistic effect, adding regulation of core hair follicle regeneration pathways such as Hippo and VEGF, and significantly enhancing the improvement effects on the immune microenvironment and circulatory system. The mechanism of action of this combination scheme achieves full coverage from "precise cell regulation" to "system environment improvement," and its effects are far superior to those of single components, demonstrating outstanding substantive characteristics and significant progress. After combination, a large number of extremely critical and specific pathways are added, greatly enriching the mechanism of action.

[0154] Test Example 2

[0155] The proliferative effect of human dermal papilla cells

[0156] The synergistic effect of the combination of plant extracts and bioactive ingredients was verified by evaluating the in vitro efficacy of human dermal papilla cell proliferation. Based on the safe dosage selected by human dermal papilla cells, the effect of the sample on cell viability after 48 h of treatment was detected to evaluate the proliferation effect of the sample on human dermal papilla cells.

[0157] Specific testing methods:

[0158] (1) Collect cells in the logarithmic growth phase, according to a cell density of 8 × 10⁻⁶. 4 / well inoculated into a 24-well culture plate.

[0159] (2) Sample loading: After culturing in an incubator (37℃, 5% CO2) for 24 h, add the drug according to Table 3.

[0160] (3) Detection: 48 h after loading the sample, the supernatant was discarded, and 500 μL of culture medium containing 0.5 mg / mL MTT was added. The sample was incubated at 37°C in the dark for 4 h. After incubation, the supernatant was discarded, and 300 μL of DMSO was added to each well. The OD value was read at 490 nm.

[0161] (4) Cell viability calculation:

[0162] Cell viability = (sample well OD - zeroing well OD) / (solvent control well OD - zeroing well OD) × 100%

[0163] The zeroing well contains only 0.5 mg / mL MTT culture medium, while the solvent control well contains cells and the same volume of solvent as the sample group.

[0164] Table 3

[0165]

[0166] Table 4

[0167]

[0168] As shown in Table 4, the anti-hair loss composition prepared by this invention can promote the proliferation of human hair papilla cells. The extracts of Platycladus orientalis leaves, peppermint leaves, and pomegranate peel have certain synergistic effects on the above-mentioned effects. Polypeptides, biotin, and amino acids also have certain synergistic effects. The simultaneous addition of plant ingredients and bioactive ingredients achieves a comprehensive anti-hair loss effect through multiple dimensions and pathways.

[0169] The above description is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention fall within the protection and disclosure scope of the present invention.

Claims

1. A hair loss prevention composition, characterized in that, The composition includes arborvitae leaf extract, peppermint leaf extract, pomegranate peel extract, polypeptides, biotin, and amino acids.

2. The anti-hair loss composition according to claim 1, characterized in that, The composition comprises, by weight parts, 0.01-10 parts of Platycladus orientalis leaf extract, 0.01-5 parts of peppermint leaf extract, 0.001-2 parts of pomegranate peel extract, 0.002-3 parts of polypeptide, 0.001-1 parts of biotin, and 0.001-5 parts of amino acids.

3. The anti-hair loss composition according to claim 1 or 2, characterized in that, The polypeptide includes tripeptide-1 and acetyl tetrapeptide-3; Preferably, the mass ratio of tripeptide-1 to acetyl tetrapeptide-3 is (0.001-1):(0.001-2).

4. The anti-hair loss composition according to any one of claims 1-3, characterized in that, The amino acid in question is sarcosine.

5. The anti-hair loss composition according to any one of claims 1-4, characterized in that, The pomegranate peel extract was prepared by a method comprising the following steps: (1) Pomegranate peel was mixed with an ethanol aqueous solution and subjected to microwave-ultrasound combined treatment to obtain the first mixture; (2) The first mixture was subjected to negative pressure cavitation extraction to obtain an extract; (3) Refrigerate the extract to coagulate, collect the supernatant, and dry it to obtain the final product.

6. The anti-hair loss composition according to claim 5, characterized in that, The temperature for the combined treatment is 40-80℃.

7. The anti-hair loss composition according to claim 5 or 6, characterized in that, The microwave processing power is 200-500 W, and the microwave processing time is 3-15 min; Preferably, the ultrasonic treatment has a power of 50-400 W and a duration of 3-10 min.

8. The anti-hair loss composition according to any one of claims 5-7, characterized in that, The negative pressure cavitation extraction is performed at a temperature of 40-70℃ for 20-40 minutes, with a vacuum degree of 60-120 mbar.

9. The anti-hair loss composition according to any one of claims 5-8, characterized in that, The refrigerated flocculation temperature is 2-8℃, and the time is 10-30 h.

10. The use of the anti-hair loss composition according to any one of claims 1-9 in the preparation of a product having anti-hair loss efficacy.

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