Antioxidant whitening composition containing fermentation liquor and preparation method thereof

Abstract

The antioxidant whitening composition is prepared from the following raw materials in parts by weight: 80 to 90 parts of water, 3 to 7 parts of butanediol, 7 to 12 parts of a compound probiotic fermentation product, 5 to 10 parts of a compound functional nano-emulsion, 5 to 10 parts of 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol, 1, 3-butanediol and 1, 3-butanediol. The composition is prepared from the following components in parts by weight: 2 to 5 parts of 1, 2-hexanediol, 2.5 to 4 parts of synergistic active matter, 0.3 to 0.5 part of p-hydroxyacetophenone, 0.3 to 0.5 part of trace functional component, 0.2 to 0.3 part of thickening agent and 0.2 to 0.3 part of EDTA (Ethylene Diamine Tetraacetic Acid) disodium. According to the antioxidant whitening composition containing the fermentation liquor, a compound probiotic fermentation product and a specific active matter system are scientifically compounded, so that source inhibition and free radical removal of oxidative stress are realized, and full-link intervention on skin pigmentation and deep protection on oxidative damage are realized; and finally, the comprehensive skin care effects of brightening skin color, improving darkness and delaying light aging are achieved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an antioxidant and whitening composition containing fermentation liquid and its preparation method. Background Technology

[0002] With rising living standards and evolving consumption concepts, consumers are increasingly demanding higher efficacy in cosmetics, with whitening and anti-oxidation remaining key market focuses. Dull skin, age spots, and photoaging are primarily caused by excessive free radical production (oxidative stress) due to factors such as UV radiation, environmental pollution, and life stress. This stress activates tyrosinase activity, promoting melanin production and deposition. Therefore, developing cosmetics with both high-efficiency anti-oxidation and whitening effects is a key research direction for the industry.

[0003] Currently, whitening and antioxidant products on the market face several key technological challenges: First, traditional whitening agents such as retinoids and hydroquinone, while highly effective, often cause significant irritation, leading to skin redness, peeling, and even allergic reactions. Milder ingredients, such as vitamin C and its derivatives, and plant extracts, often suffer from poor stability, easy oxidation and discoloration, or low transdermal absorption. For example, polyphenols with excellent antioxidant activity, such as resveratrol and ferulic acid, are difficult to maintain their activity in formulations due to their poor water solubility and sensitivity to light and heat. They also struggle to penetrate the stratum corneum to reach their target sites, limiting their practical application. Therefore, improving the stability and bioavailability of these poorly soluble and unstable active ingredients through carrier technology is a current challenge in formulation research. Second, the concept of microecological skincare has gained traction in recent years. Research indicates that the balance of the skin's surface microecological system is closely related to skin barrier function, inflammatory responses, and pigment metabolism. Traditional skincare formulas often neglect the maintenance of the skin's microbiome, and may even disrupt the skin's flora balance due to excessive use of preservatives or strong surfactants, leading to a damaged skin barrier and exacerbating skin sensitivity and dullness. Although there are products on the market that contain probiotic fermentation liquid, many of these products are merely conceptual additions, lacking a scientifically formulated blend of multiple strains. Alternatively, the complex composition of the fermentation liquid itself makes it difficult for it to coexist stably with other highly active ingredients in the formula (such as whitening agents and antioxidants), resulting in stability issues such as product separation, sedimentation, or off-flavors.

[0004] Furthermore, to achieve the encapsulation and delivery of active ingredients, existing nanocarrier technologies (such as liposomes and nanocapsules) often utilize large amounts of synthetic surfactants or organic polymers. This not only increases the complexity and cost of formulations but may also introduce additional risks of skin irritation. Finding biocompatible, green, and safe natural polymers as carrier matrices is also one of the urgent problems to be solved in this field.

[0005] In conclusion, developing a highly effective antioxidant and whitening composition that can maintain the skin's microecological balance, effectively solve the problems of active ingredient stability and transdermal absorption, and is gentle and low in irritation has significant market value and application prospects. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide an antioxidant whitening composition containing fermentation broth and its preparation method.

[0007] To achieve the above objectives, the present invention provides the following technical solution: An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 80-90 parts water, 3-7 parts butylene glycol, 7-12 parts compound probiotic fermentation product, 5-10 parts compound functional nanoemulsion, 2-5 parts 1,2-hexanediol, 2.5-4 parts synergistic active ingredient, 0.3-0.5 parts p-hydroxyacetophenone, 0.3-0.5 parts trace functional ingredients, 0.2-0.3 parts thickener, and 0.2-0.3 parts disodium EDTA.

[0008] Preferably, an antioxidant whitening composition containing fermentation broth comprises, by weight, the following raw materials: 82-88 parts water, 4-6 parts butylene glycol, 8-11 parts compound probiotic fermentation product, 6-9 parts compound functional nanoemulsion, 3-5 parts 1,2-hexanediol, 3-4 parts synergistic active ingredient, 0.3-0.4 parts p-hydroxyacetophenone, 0.3-0.4 parts trace functional ingredients, 0.25-0.3 parts thickener, and 0.2-0.25 parts disodium EDTA.

[0009] Preferably, the thickener is one or two of xanthan gum and carbomer; the trace functional components are composed of panthenol, carnosine, and bisabolol in a mass ratio of 4-6:0.5-1.5:0.2-0.5.

[0010] Preferably, the compound probiotic fermentation product is composed of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 3-5:2-3:1-2:1-2; the synergistic active ingredient is composed of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2-3:0.5-1.

[0011] Preferably, the preparation method of the composite functional nanoemulsion includes the following steps: S1. Add sodium hyaluronate to deionized water, then add sodium periodate, adjust the pH to 4-5, stir the reaction under light-protected conditions for a preset time, add ethylene glycol to terminate the reaction, and then perform ultrafiltration to obtain a modified sodium hyaluronate solution; then add silk fibroin and glycerin to the modified sodium hyaluronate solution, stir evenly, and adjust the pH to 7.5-8.5 to obtain a prepolymer aqueous solution; S2. Resveratrol, α-arbutin, soybean lecithin, vitamin E and ferulic acid were added to anhydrous ethanol and stirred at 40°C in the dark to obtain a drug-loaded organic phase solution. S3. Under high-speed stirring, the drug-loaded organic phase solution from step S2 is rapidly injected into the prepolymer aqueous solution from step S1 to form a nanoemulsion. Then, the stirring speed is reduced and the reaction is heated. After the reaction is completed, ethanol is removed by rotary evaporation and the solution is concentrated to 75-85% of the volume of the prepolymer aqueous solution. The solution is then filtered to remove bacteria.

[0012] Preferably, in step S1, the molecular weight of sodium hyaluronate is 800kDa-1200kDa, the mass ratio of sodium hyaluronate to sodium periodate is 3-5:1, the amount of ethylene glycol added is 0.5-1 times the mass of sodium periodate, the temperature of the stirring reaction is 20-25℃, and the time is 1-2h, and the ultrafiltration uses an ultrafiltration membrane with a molecular weight cutoff of 3000-5000Da.

[0013] Preferably, in step S1, the mass concentration of the modified sodium hyaluronate solution is 1-2%, the amount of silk fibroin added is 2-3% of the mass of the modified sodium hyaluronate solution, and the amount of glycerol added is 15-20% of the mass of the silk fibroin.

[0014] In this invention, sodium periodate is used to controllably oxidize large molecular weight sodium hyaluronate under mild acidic conditions to introduce chemically active sites—aldehyde groups. Subsequently, the purified modified sodium hyaluronate is compounded with amino-rich silk fibroin and prepared in a weakly alkaline environment to prepare for subsequent chemical cross-linking reactions.

[0015] This invention uses sodium hyaluronate and silk fibroin as raw materials. The excellent biocompatibility of sodium hyaluronate and silk fibroin ensures that the nanocarrier can be accepted by the skin in a friendly manner, reducing irritation, which is a prerequisite for the efficacy. More importantly, the reactive framework created through chemical modification lays the foundation for the ultra-high stability of the final nanoemulsion, ensuring that sufficient and highly active ingredients can reach the target site.

[0016] Preferably, the mass ratio of resveratrol, α-arbutin, soybean lecithin, vitamin E, ferulic acid, and anhydrous ethanol in step S2 is 2:1.5-2:5-8:1-2:0.5-1:100-120, and the stirring time in the dark is 30-40 minutes.

[0017] In this invention, resveratrol, vitamin E, ferulic acid, and α-arbutin are dissolved in ethanol and thoroughly mixed with amphiphilic soybean lecithin to prepare for the next step.

[0018] This invention pre-encapsulates resveratrol, which is extremely sensitive and easily decomposes in light, with vitamin E and ferulic acid, which can synergistically enhance and protect it, forming a powerful antioxidant core. This core is not only the main source of antioxidant efficacy, but also, through the encapsulation of soybean lecithin, initially isolates it from external oxygen and light, maximizing the preservation of the original activity of the active ingredients before encapsulation. Furthermore, the whitening agent α-arbutin is also introduced, realizing the encapsulation of water-soluble whitening ingredients and oil-soluble antioxidant ingredients in the same nanoemulsion, solving the problem that the two are difficult to synergize effectively in conventional formulas, and improving the whitening efficacy of the product.

[0019] Preferably, in step S3, the mass ratio of the prepolymer aqueous solution to the drug-loaded organic phase solution is 4-5:1; the high-speed stirring speed is 8000-10000 r / min, and the system temperature is controlled at 10-15℃ during the injection process; the stirring speed is reduced by adjusting the speed to 300-500 r / min; the heating reaction temperature is 40-45℃, and the time is 1.5-2.5 h; the rotary evaporation temperature is 35-40℃, and the vacuum degree is -(0.08~0.1) MPa.

[0020] In this invention, an oil phase solution is injected into an aqueous phase framework by high-speed shearing to induce the formation of a preliminary nanoemulsion. Subsequently, by gentle heating, modified sodium hyaluronate in the aqueous phase undergoes a Schiff base chemical reaction with silk fibroin to form a covalent cross-linked network on the surface of the nanoemulsion. Finally, the organic solvent is removed and purified by rotary evaporation and filtration to obtain the final high-concentration, high-purity composite functional nanoemulsion.

[0021] This invention forms a robust chemically cross-linked shell, preventing the active ingredients from being released rapidly and instead allowing them to be released slowly and continuously. Furthermore, for potent but potentially irritating ingredients like resveratrol, sustained release significantly improves skin tolerance, making the effects gentler. Moreover, the slow, continuous release allows the skin to remain protected by antioxidants and whitening agents for an extended period, thus significantly prolonging the product's effective duration and extending its antioxidant and whitening effects.

[0022] This invention also protects a method for preparing an antioxidant whitening composition containing fermentation broth as described above, comprising the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 80-85℃, stir for 30-40 minutes, then cool to 50-60℃, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 10-20 minutes, continue to cool to 40-45℃, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 20-30 minutes, adjust the pH to 5.5-6.5, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0023] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention provides an antioxidant whitening composition containing fermentation liquid, which achieves source inhibition of oxidative stress and free radical scavenging by scientifically compounding compound probiotic fermentation products with a specific active ingredient system; at the same time, the composite functional nanoemulsion prepared by a specific method efficiently encapsulates oil-soluble antioxidant (resveratrol) and water-soluble whitening agent (α-arbutin), significantly improving the stability, transdermal penetration efficiency and bioavailability of the active ingredients; the components of the composition work synergistically to achieve full-link intervention on skin pigmentation and deep protection against oxidative damage under the premise of gentleness and non-irritation, and finally achieve comprehensive skin care effects of brightening skin tone, improving dullness and delaying photoaging.

[0024] (2) The present invention provides an antioxidant whitening composition containing fermentation broth, which is a composite functional nanoemulsion prepared by a specific method. Addressing the technical challenges of poor water solubility, low photothermal stability, and difficulty in transdermal absorption of active ingredients such as resveratrol and ferulic acid, the present invention innovatively uses sodium periodate to oxidize and modify sodium hyaluronate to introduce aldehyde groups. It then utilizes the Schiff base reaction to form a cross-linking network with the amino groups in silk fibroin, thus forming a core-shell structured nanoemulsion. The hydrophilic modified hyaluronic acid shell endows the system with excellent water dispersibility and moisturizing properties, not only avoiding the oxidative inactivation of active ingredients but also utilizing the specific binding of hyaluronic acid to skin cells to achieve the desired effect. The delivery and long-lasting release of the active ingredients significantly improve their bioavailability. Furthermore, during the preparation of the nanoemulsion, the ratio of the prepolymer aqueous solution to the drug-loaded organic phase solution (3-5:1) is strictly controlled, avoiding the use of large amounts of surfactants in traditional emulsification processes. Under high-speed shearing, the organic phase disperses into tiny droplets, followed by ethanol diffusion into the aqueous phase, inducing modified hyaluronic acid and silk fibroin to rapidly self-assemble into a film at the oil-water interface. Subsequent heating further solidifies the cross-linked structure, and rotary evaporation removes the organic solvent. The resulting nanoemulsion exhibits uniform particle size, good stability, and no organic solvent residue, resulting in higher safety.

[0025] (3) The present invention provides an antioxidant whitening composition containing fermentation liquid. The compound probiotic fermentation products selected in the present invention include metabolites and lysates of Lactobacillus, Bifida Ferment Lysate and Streptococcus thermophilus. These components are transformed from macromolecules into small molecule peptides, organic acids and extracellular polysaccharides that are more easily absorbed by the skin through fermentation process. In particular, the lysate of Bifida Ferment Lysate fermentation product contains rich cytoplasm and cell wall components, which can effectively promote DNA repair mechanism and resist skin photoaging caused by ultraviolet rays. Combined with the organic acid environment produced by Lactobacillus fermentation product, it helps to optimize the pH of the skin surface. The formula inhibits the growth of harmful bacteria, thus significantly improving skin smoothness and radiance while whitening. Furthermore, the synergistic active ingredients (niacinamide and 3-o-ethyl ascorbic acid) in the formula complement the α-arbutin in the nanoemulsion. α-Arbutin inhibits tyrosinase activity at its source, niacinamide blocks the transport of melanin to keratinocytes, and 3-o-ethyl ascorbic acid reduces existing melanin precursors. Through the synergistic effect of multiple ingredients, over-reliance on a single whitening pathway is avoided, thereby greatly reducing the potential risk of skin irritation while ensuring efficacy. Attached Figure Description

[0026] Figure 1 The effects of various embodiments and comparative examples of the present invention on the melanin content of B16-F10 cells. Detailed Implementation

[0027] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0028] Unless otherwise specified, all chemical reagents and materials in this invention are purchased from the market or synthesized from raw materials purchased from the market.

[0029] Example 1 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 85 parts water, 5 parts butylene glycol, 10 parts compound probiotic fermentation product, 8 parts compound functional nanoemulsion, 3 parts 1,2-hexanediol, 3.5 parts synergistic active ingredient, 0.4 parts p-hydroxyacetophenone, 0.4 parts trace functional ingredients, 0.25 parts carbomer, and 0.25 parts disodium EDTA.

[0030] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 5:1:0.4; the compound probiotic fermentation product consists of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 4:2.5:1.5:1.5; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2.5:0.8.

[0031] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1.2g of sodium periodate, adjust the pH to 4.5, and stir the reaction at 23℃ for 1.5h under light-protected conditions. Add 1g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000Da to obtain a modified sodium hyaluronate solution with a mass concentration of 1.5%. Then add 13g of silk fibroin and 2.5g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 8 to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 1.8g α-arbutin, 7g soybean lecithin, 1.5g vitamin E and 0.8g ferulic acid to 100g anhydrous ethanol, and stir at 40℃ in the dark for 35min to obtain a drug-loaded organic phase solution. S3. Under a stirring speed of 9000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 450 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 10 min to form a nanoemulsion. Then, the stirring speed is reduced to 400 r / min, and the mixture is reacted at 43 °C for 2 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 40 °C and a vacuum degree of -0.09 MPa. The mixture is then concentrated to 80% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0032] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 35 minutes, then cool to 55°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 15 minutes, continue to cool to 40°C, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 25 minutes, adjust the pH to 6, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0033] Example 2 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 80 parts water, 3 parts butylene glycol, 7 parts compound probiotic fermentation product, 5 parts compound functional nanoemulsion, 2 parts 1,2-hexanediol, 2.5 parts synergistic active ingredient, 0.3 parts p-hydroxyacetophenone, 0.3 parts trace functional ingredients, 0.2 parts carbomer, and 0.2 parts disodium EDTA.

[0034] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 4:0.5:0.2; the compound probiotic fermentation product consists of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 3:2:1:1; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2:0.5.

[0035] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1g of sodium periodate, adjust the pH to 4, and stir the reaction at 20°C for 2 hours under light-protected conditions. Add 0.5g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000 Da to obtain a modified sodium hyaluronate solution with a mass concentration of 1%. Then add 10g of silk fibroin and 1.5g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 7.5 to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 1.5g α-arbutin, 5g soybean lecithin, 1g vitamin E and 0.5g ferulic acid to 100g anhydrous ethanol, stir at 40℃ in the dark for 30min to obtain drug-loaded organic phase solution. S3. Under a stirring speed of 8000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 400 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 5 min to form a nanoemulsion. Then, the stirring speed is reduced to 300 r / min, and the mixture is reacted at 40 °C for 2.5 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 35-40 °C and a vacuum degree of -0.08 MPa. The mixture is then concentrated to 75% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0036] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 80℃, stir for 40 minutes, then cool to 50℃, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 20 minutes, continue to cool to 40℃, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 30 minutes, adjust the pH to 5.5, filter through a 200-mesh sieve, let stand to remove bubbles, and the product is obtained.

[0037] Example 3 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 90 parts water, 7 parts butylene glycol, 12 parts compound probiotic fermentation product, 10 parts compound functional nanoemulsion, 5 parts 1,2-hexanediol, 4 parts synergistic active ingredient, 0.5 parts p-hydroxyacetophenone, 0.5 parts trace functional ingredients, 0.3 parts carbomer, and 0.3 parts disodium EDTA.

[0038] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 6:1.5:0.5; the compound probiotic fermentation product consists of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 5:3:2:2; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 3:1.

[0039] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1.5g of sodium periodate, adjust the pH to 5, and stir the reaction at 25℃ for 1h under light-protected conditions. Add 1.5g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000Da to obtain a modified sodium hyaluronate solution with a mass concentration of 2%. Then add 15g of silk fibroin and 3g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 8.5 to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 2g α-arbutin, 8g soybean lecithin, 2g vitamin E and 1g ferulic acid to 100g anhydrous ethanol, stir at 40℃ in the dark for 40min to obtain drug-loaded organic phase solution. S3. Under a stirring speed of 10000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 500 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 20 min to form a nanoemulsion. Then, the stirring speed is reduced to 500 r / min, and the mixture is reacted at 45 °C for 1.5 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 40 °C and a vacuum degree of -0.1 MPa. The mixture is then concentrated to 85% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0040] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 30 minutes, then cool to 60°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 10 minutes, continue to cool to 45°C, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 20 minutes, adjust the pH to 6.5, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0041] Comparative Example 1 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 85 parts water, 5 parts butylene glycol, 10 parts compound probiotic fermentation product, 8 parts compound functional nanoemulsion, 3 parts 1,2-hexanediol, 3.5 parts synergistic active ingredient, 0.4 parts p-hydroxyacetophenone, 0.4 parts trace functional ingredients, 0.25 parts carbomer, and 0.25 parts disodium EDTA.

[0042] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 5:1:0.4; the compound probiotic fermentation product consists of Lactobacillus fermentation product and Bifida ferment filtrate in a mass ratio of 4:2.5; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2.5:0.8.

[0043] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1.2g of sodium periodate, adjust the pH to 4.5, and stir the reaction at 23℃ for 1.5h under light-protected conditions. Add 1g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000Da to obtain a modified sodium hyaluronate solution with a mass concentration of 1.5%. Then add 13g of silk fibroin and 2.5g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 8 to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 1.8g α-arbutin, 7g soybean lecithin, 1.5g vitamin E and 0.8g ferulic acid to 100g anhydrous ethanol, and stir at 40℃ in the dark for 35min to obtain a drug-loaded organic phase solution. S3. Under a stirring speed of 9000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 450 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 10 min to form a nanoemulsion. Then, the stirring speed is reduced to 400 r / min, and the mixture is reacted at 43 °C for 2 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 40 °C and a vacuum degree of -0.09 MPa. The mixture is then concentrated to 80% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0044] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 35 minutes, then cool to 55°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 15 minutes, continue to cool to 40°C, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 25 minutes, adjust the pH to 6, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0045] Compared with Example 1, the compound probiotic fermentation product in this comparative example did not contain Streptococcus thermophilus fermentation product, Bifida ferment lysate, or cell lysate.

[0046] Comparative Example 2 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 85 parts water, 5 parts butylene glycol, 10 parts compound probiotic fermentation product, 8 parts compound functional nanoemulsion, 3 parts 1,2-hexanediol, 0.4 parts p-hydroxyacetophenone, 0.4 parts trace functional ingredients, 0.25 parts carbomer, and 0.25 parts disodium EDTA.

[0047] The trace functional components are composed of panthenol, carnosine, and bisabolol in a mass ratio of 5:1:0.4; the compound probiotic fermentation product is composed of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 4:2.5:1.5:1.5.

[0048] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1.2g of sodium periodate, adjust the pH to 4.5, and stir the reaction at 23℃ for 1.5h under light-protected conditions. Add 1g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000Da to obtain a modified sodium hyaluronate solution with a mass concentration of 1.5%. Then add 13g of silk fibroin and 2.5g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 8 to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 1.8g α-arbutin, 7g soybean lecithin, 1.5g vitamin E and 0.8g ferulic acid to 100g anhydrous ethanol, and stir at 40℃ in the dark for 35min to obtain a drug-loaded organic phase solution. S3. Under a stirring speed of 9000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 450 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 10 min to form a nanoemulsion. Then, the stirring speed is reduced to 400 r / min, and the mixture is reacted at 43 °C for 2 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 40 °C and a vacuum degree of -0.09 MPa. The mixture is then concentrated to 80% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0049] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 35 minutes, then cool to 55°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 15 minutes, continue to cool to 40°C, add the compound probiotic fermentation product and the compound functional nanoemulsion trace functional ingredients, stir for 25 minutes, adjust the pH to 6, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0050] Compared with Example 1, no synergistic active ingredient was added to this comparative example.

[0051] Comparative Example 3 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 85 parts water, 5 parts butylene glycol, 10 parts compound probiotic fermentation product, 8 parts compound functional nanoemulsion, 3 parts 1,2-hexanediol, 3.5 parts synergistic active ingredient, 0.4 parts p-hydroxyacetophenone, 0.4 parts trace functional ingredients, 0.25 parts carbomer, and 0.25 parts disodium EDTA.

[0052] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 5:1:0.4; the compound probiotic fermentation product consists of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 4:2.5:1.5:1.5; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2.5:0.8.

[0053] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 1000g of deionized water, then add 1.2g of sodium periodate, adjust the pH to 4.5, and stir the reaction at 23℃ for 1.5h under light-protected conditions. Add 1g of ethylene glycol to terminate the reaction, and then concentrate the solution by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 4000Da to obtain a modified sodium hyaluronate solution with a mass concentration of 1.5%. Then add 13g of silk fibroin and 2.5g of glycerol to 500g of modified sodium hyaluronate solution, stir evenly, and adjust the pH to 8 to obtain a prepolymer aqueous solution. S2. Add 2g of resveratrol, 7g of soybean lecithin, and 1.5g of vitamin E to 100g of anhydrous ethanol, and stir at 40°C in the dark for 35 minutes to obtain a drug-loaded organic phase solution. S3. Under a stirring speed of 9000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 450 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 10 min to form a nanoemulsion. Then, the stirring speed is reduced to 400 r / min, and the mixture is reacted at 43 °C for 2 h. After the reaction is complete, ethanol is removed by rotary evaporation at a temperature of 40 °C and a vacuum degree of -0.09 MPa. The mixture is then concentrated to 80% of the volume of the prepolymer aqueous solution. Finally, the mixture is filtered through a 0.45 μm filter membrane to remove impurities, thus obtaining the final product.

[0054] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 35 minutes, then cool to 55°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 15 minutes, continue to cool to 40°C, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 25 minutes, adjust the pH to 6, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0055] Compared with Example 1, no α-arbutin and ferulic acid were added to the composite functional nanoemulsion in this comparative example.

[0056] Comparative Example 4 An antioxidant whitening composition containing fermentation broth, comprising the following ingredients in parts by weight: 85 parts water, 5 parts butylene glycol, 10 parts compound probiotic fermentation product, 8 parts compound functional nanoemulsion, 3 parts 1,2-hexanediol, 3.5 parts synergistic active ingredient, 0.4 parts p-hydroxyacetophenone, 0.4 parts trace functional ingredients, 0.25 parts carbomer, and 0.25 parts disodium EDTA.

[0057] The trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 5:1:0.4; the compound probiotic fermentation product consists of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 4:2.5:1.5:1.5; and the synergistic active ingredient consists of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2.5:0.8.

[0058] The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add 5g of sodium hyaluronate to 750g of deionized water, then add 13g of silk fibroin and 2.5g of glycerol. Stir well to obtain a prepolymer aqueous solution. S2. Add 2g resveratrol, 1.8g α-arbutin, 7g soybean lecithin, 1.5g vitamin E and 0.8g ferulic acid to 100g anhydrous ethanol, and stir at 40℃ in the dark for 35min to obtain a drug-loaded organic phase solution. S3. Under a stirring speed of 9000 r / min, 100 g of the drug-loaded organic phase solution from step S2 is rapidly injected into 450 g of the prepolymer aqueous solution from step S1. The mixture is continuously sheared for 10 min to form a nanoemulsion. Ethanol is then removed by rotary evaporation at a temperature of 40 °C and a vacuum of -0.09 MPa. The solution is then concentrated to 80% of the volume of the prepolymer aqueous solution. Finally, the solution is filtered through a 0.45 μm filter membrane to remove impurities, yielding the final product.

[0059] A method for preparing an antioxidant whitening composition containing fermentation broth includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 85°C, stir for 35 minutes, then cool to 55°C, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 15 minutes, continue to cool to 40°C, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 25 minutes, adjust the pH to 6, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

[0060] Compared with Example 1, the composite functional nanoemulsion in this comparative example did not modify sodium hyaluronate.

[0061] The antioxidant whitening compositions containing fermentation broth prepared in Examples 1-3 and Comparative Examples 1-4 were tested for efficacy. The test results are shown in Table 1 below.

[0062] (1) Determination of DPPH free radical scavenging ability The antioxidant whitening compositions containing fermentation broth prepared in Examples 1-3 and Comparative Examples 1-4 were each added to 1 mL of an ethanol solution containing 40 μmol / L DPPH free radicals. After standing at 25°C for 30 min, the absorbance values ​​of blank (A) and sample (A) were measured at 517 nm. The formula for calculating the DPPH free radical scavenging ability is as follows: DPPH free radical scavenging capacity (%) = (1-A) 样品 / A 空白 )×100 After storing the antioxidant whitening compositions containing fermentation broth from Examples 1-3 and Comparative Examples 1-4 under natural light for 2 months, the DPPH free radical scavenging rate was tested according to the above method.

[0063] (2) Tyrosinase inhibitory activity was tested in accordance with T / SHRH 015-2018.

[0064] (3) Effect on melanin content of B16-F10 cells: B16-F10 cells were seeded in culture dishes or culture flasks and cultured in DMEM medium containing 10% fetal bovine serum at 37°C and 5% CO2. The cells were used for experiments after reaching the logarithmic growth phase.

[0065] The experimental groups used Examples 1-3 and Comparative Examples 1-4, with a blank control group included. The antioxidant whitening compositions containing fermentation broth prepared in Examples 1-3 and Comparative Examples 1-4 were added to each experimental group. After incubation for 24 hours, the culture medium was discarded, and the cells were washed 2-3 times with PBS. Cell lysis buffer was added, and the cells were incubated at room temperature for 2 hours to allow for complete cell lysis and release of melanin. The cell lysis buffer was transferred to centrifuge tubes, centrifuged, and the supernatant was collected. The absorbance of the supernatant was measured at 475 nm using spectrophotometry. The relative change in melanin content in each experimental group relative to the blank control group was calculated: relative melanin content (%) = absorbance of experimental group / absorbance of blank control group × 100%. The results are shown below. Figure 1 .

[0066] Table 1 From Table 1 and Figure 1 As can be seen, the antioxidant whitening composition containing fermentation broth prepared in this invention has good antioxidant effect, and also inhibits the activity of tyrosinase, reduces the phase difference content of melanin, and has excellent whitening effect, while also having good antioxidant stability.

[0067] The above description is a further detailed explanation of the present invention in conjunction with specific implementation examples. It should not be considered that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, several simple deductions or substitutions can be made without departing from the concept of the present invention, and all such deductions or substitutions should be considered to fall within the protection scope of the present invention.

[0068] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An antioxidant and whitening composition containing fermentation broth, characterized in that, By weight, it includes the following ingredients: 80-90 parts water, 3-7 parts butylene glycol, 7-12 parts compound probiotic fermentation product, 5-10 parts compound functional nanoemulsion, 2-5 parts 1,2-hexanediol, 2.5-4 parts synergistic active ingredient, 0.3-0.5 parts p-hydroxyacetophenone, 0.3-0.5 parts trace functional ingredients, 0.2-0.3 parts thickener, and 0.2-0.3 parts disodium EDTA.

2. The antioxidant whitening composition containing fermentation broth according to claim 1, characterized in that, By weight, it includes the following ingredients: 82-88 parts water, 4-6 parts butylene glycol, 8-11 parts compound probiotic fermentation product, 6-9 parts compound functional nanoemulsion, 3-5 parts 1,2-hexanediol, 3-4 parts synergistic active ingredient, 0.3-0.4 parts p-hydroxyacetophenone, 0.3-0.4 parts trace functional ingredients, 0.25-0.3 parts thickener, and 0.2-0.25 parts disodium EDTA.

3. The antioxidant whitening composition containing fermentation broth according to claim 1, characterized in that, The thickener is one or both of xanthan gum and carbomer; the trace functional components consist of panthenol, carnosine, and bisabolol in a mass ratio of 4-6:0.5-1.5:0.2-0.

5.

4. The antioxidant whitening composition containing fermentation broth according to claim 1, characterized in that, The compound probiotic fermentation product is composed of Lactobacillus fermentation product, Bifida ferment filtrate, Streptococcus thermophilus fermentation product, and Bifida ferment lysate in a mass ratio of 3-5:2-3:1-2:1-2; the synergistic active ingredient is composed of nicotinamide and 3-o-ethyl ascorbic acid in a mass ratio of 2-3:0.5-1.

5. The antioxidant whitening composition containing fermentation broth according to claim 1, characterized in that, The preparation method of the composite functional nanoemulsion includes the following steps: S1. Add sodium hyaluronate to deionized water, then add sodium periodate, adjust the pH to 4-5, stir the reaction under light-protected conditions for a preset time, add ethylene glycol to terminate the reaction, and then perform ultrafiltration to obtain a modified sodium hyaluronate solution; then add silk fibroin and glycerin to the modified sodium hyaluronate solution, stir evenly, and adjust the pH to 7.5-8.5 to obtain a prepolymer aqueous solution; S2. Resveratrol, α-arbutin, soybean lecithin, vitamin E and ferulic acid were added to anhydrous ethanol and stirred at 40°C in the dark to obtain a drug-loaded organic phase solution. S3. Under high-speed stirring, the drug-loaded organic phase solution from step S2 is rapidly injected into the prepolymer aqueous solution from step S1 to form a nanoemulsion. Then, the stirring speed is reduced and the reaction is heated. After the reaction is completed, ethanol is removed by rotary evaporation and the solution is concentrated to 75-85% of the volume of the prepolymer aqueous solution. After filtration to remove impurities, the final product is obtained.

6. The antioxidant whitening composition containing fermentation broth according to claim 5, characterized in that, In step S1, the molecular weight of sodium hyaluronate is 800kDa-1200kDa, and the mass ratio of sodium hyaluronate to sodium periodate is 3-5:1; the amount of ethylene glycol added is 0.5-1 times the mass of sodium periodate; the temperature of the stirring reaction is 20-25℃, and the time is 1-2h; the ultrafiltration uses an ultrafiltration membrane with a molecular weight cutoff of 3000-5000Da.

7. The antioxidant whitening composition containing fermentation broth according to claim 5, characterized in that, In step S1, the mass concentration of the modified sodium hyaluronate solution is 1-2%, the amount of silk fibroin added is 2-3% of the mass of the modified sodium hyaluronate solution, and the amount of glycerol added is 15-20% of the mass of the silk fibroin.

8. The antioxidant whitening composition containing fermentation broth according to claim 5, characterized in that, In step S2, the mass ratio of resveratrol, α-arbutin, soybean lecithin, vitamin E, ferulic acid, and anhydrous ethanol is 2:1.5-2:5-8:1-2:0.5-1:100-120, and the stirring time in the dark is 30-40 minutes.

9. The antioxidant whitening composition containing fermentation broth according to claim 5, characterized in that, In step S3, the mass ratio of the prepolymer aqueous solution to the drug-loaded organic phase solution is 4-5:1; the high-speed stirring speed is 8000-10000 r / min, and the system temperature is controlled at 10-15℃ during the injection process; the stirring speed is reduced to 300-500 r / min; the heating reaction temperature is 40-45℃, and the time is 1.5-2.5 h; the rotary evaporation temperature is 35-40℃, and the vacuum degree is -(0.08~0.1) MPa.

10. A method for preparing an antioxidant whitening composition containing fermentation broth as described in any one of claims 1-9, characterized in that, Includes the following steps: Weigh the raw materials according to the formula, add the thickener and disodium EDTA to water, heat to 80-85℃, stir for 30-40 minutes, then cool to 50-60℃, add butylene glycol, 1,2-hexanediol and p-hydroxyacetophenone, stir for 10-20 minutes, continue to cool to 40-45℃, add compound probiotic fermentation products, compound functional nanoemulsion, synergistic active ingredients and trace functional ingredients, stir for 20-30 minutes, adjust the pH to 5.5-6.5, filter through a 200-mesh sieve, and let stand to remove bubbles to obtain the final product.

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