Targeted multi-channel synergistic anti-aging composition as well as preparation method and application thereof

Abstract

The invention discloses a targeted multi-channel synergistic anti-aging composition as well as a preparation method and application thereof, and belongs to the technical field of functional foods and biological medicines. According to the composition disclosed by the invention, ginsenoside, a hawthorn polyphenol-chitosan phospholipid complex and spina date seed total saponins are taken as core active ingredients, and a specific Senlytics active ingredient and a natural mTOR inhibitor are compounded, so that the synergistic regulation of five anti-aging pathways, namely Sirtuins activation, mTOR inhibition, mitochondrial autophagy activation, CD38 inhibition and senescent cell removal, can be realized at the same time. The composition is natural in raw material source, high in safety and good in stability, can be prepared into various dosage forms such as capsules, solid beverages and oral liquid, is suitable for daily anti-aging and sub-health conditioning, and has wide application prospects in the fields of anti-aging health foods and biological medicines.

Description

Technical Field

[0001] This invention belongs to the field of functional food and biomedicine technology, specifically relating to a composition that can simultaneously regulate five major anti-aging pathways: Sirtuins activation, mTOR inhibition, mitophagy activation, CD38 inhibition, and senescent cell clearance, as well as the preparation method of the composition and its application in the preparation of anti-aging health foods. Background Technology

[0003] Aging is a progressive decline in the body's physiological functions over time, characterized by typical aging features such as genomic instability, telomere depletion, mitochondrial dysfunction, and cellular senescence. Modern anti-aging research has shifted from single-target intervention to multi-pathway synergistic regulation, with five core anti-aging pathways widely confirmed by domestic and international research to be closely related to healthy lifespan: 1. Sirtuins activation: The Sirtuins family (SIRT1-SIRT7) are NAD+-dependent deacetylases that participate in regulating DNA repair, metabolic homeostasis, and the body's stress resistance. Their activity decreases significantly with age. 2. Inhibition of mTOR signaling pathway: target of rapamycin (mTOR) is a core regulator of cell growth and metabolism. Its overactivation can inhibit the process of autophagy and accelerate aging. Moderate inhibition of the mTOR pathway can prolong the lifespan of various model organisms. 3. Activation of mitophagy: Mitochondrial dysfunction is one of the core markers of aging. Mitophagy, the process of selectively clearing damaged mitochondria, is a key mechanism for maintaining cellular energy metabolism homeostasis. 4. CD38 inhibition: CD38 is a major NAD+ hydrolase, and its expression level is upregulated with the aging process, which directly leads to the depletion of the intracellular NAD+ pool and thus reduces the activity of Sirtuins family proteins. 5. Senescent cell clearance (Senolytics): Senescent cells disrupt the tissue microenvironment by secreting senescence-associated secretory phenotypes (SASPs). Selective clearance of senescent cells can effectively delay the occurrence of various senescence-related pathological phenotypes.

[0004] Existing anti-aging products are mostly limited to single-pathway intervention, such as supplementing only NAD+ precursors or using only a single antioxidant, which cannot achieve systematic and synergistic regulation of the five core anti-aging pathways. Although some natural products have the potential for multi-target action, they have technical defects such as unclear mechanisms of action and low efficiency of simultaneous regulation of multiple pathways.

[0005] Therefore, developing a composition with a natural source, a clear mechanism of action, and the ability to synergistically regulate the five major anti-aging pathways is of great scientific significance and market application value for promoting the development of anti-aging functional foods and the biopharmaceutical field. Summary of the Invention

[0007] I. Purpose of the Invention The purpose of this invention is to provide a multi-component synergistic anti-aging composition, which solves the technical problem that existing anti-aging intervention methods are singular and cannot achieve systematic multi-pathway synergistic regulation; at the same time, it provides a method for preparing the composition and its application in anti-aging products.

[0008] Technical solution

[0009] To achieve the above-mentioned objectives, this invention provides a composition for targeted multi-pathway synergistic anti-aging, the active ingredients of which consist of the following components: Ginsenosides are used as Sirtuins activators; Hawthorn polyphenol-chitosan phospholipid complex, used to inhibit CD38 activity; Total saponins from jujube seed are used as mitochondrial autophagy activators; Senolytics active ingredients are selected from at least one of quercetin, flavonoids, or fisetin, and are used to eliminate senescent cells; Natural mTOR inhibitors, selected from at least one of resveratrol, curcumin, or rapamycin analogues, are used to inhibit the mTOR signaling pathway.

[0010] The present invention also provides a method for preparing the above-mentioned anti-aging composition, and the application of the composition in the preparation of anti-aging health foods and pharmaceuticals.

[0011] III. Preferred Technical Solution 1. The hawthorn polyphenol-chitosan phospholipid complex is prepared by phosphorylation reaction, with a particle size range of 50-200 nm and a zeta potential of +20 mV to +30 mV, which can significantly improve the bioavailability and targeting of the components; 2. The total content of ginsenosides is not less than 80%, of which the proportion of ginsenosides Re and Rg1 is not less than 40%; 3. The weight ratio of each active ingredient in the composition is as follows: 5-20 parts of ginsenosides, 10-30 parts of hawthorn polyphenol-chitosan phospholipid complex, 5-20 parts of total saponins from jujube seed, 3-10 parts of senolytics active ingredient, and 3-10 parts of natural mTOR inhibitor.

[0012] IV. Beneficial Effects of the Invention 1. Synergistic effect of multiple pathways: This invention is the first to integrate five core anti-aging pathways, namely Sirtuins activation, mTOR inhibition, mitophagy activation, CD38 inhibition, and senescent cell clearance, into a single formula. The synergistic effect of each component produces an anti-aging effect of 1+1>2, achieving systemic anti-aging. 2. Clear mechanism of action: Each active ingredient in the composition corresponds to a specific molecular target, which solves the technical defect of unclear mechanism of action of traditional Chinese medicine compound prescriptions; 3. High safety: The core active ingredients are all derived from medicinal and edible plants such as ginseng, hawthorn, and jujube seed. The compound ingredients are all well-researched natural small molecules with low toxicity and side effects, making them suitable for long-term daily use. 4. Excellent stability and bioavailability: By preparing hawthorn polyphenol-chitosan phospholipid complex, the common problems of easy oxidation, poor stability and low bioavailability of polyphenols in the industry are solved. Detailed Implementation

[0014] The present invention will be further described in detail below with reference to specific embodiments, but the scope of protection of the present invention is not limited to the following embodiments.

[0015] Example 1: Preparation of Anti-aging Soft Capsules 1. Raw material preparation (parts by weight): 15 parts of ginsenosides (purity ≥80%), 25 parts of hawthorn polyphenol-chitosan phospholipid complex, 18 parts of total saponins from jujube seed (purity ≥60%), 8 parts of quercetin, 6 parts of resveratrol, and appropriate amounts of soybean oil, gelatin, and glycerin excipients. The preparation method of hawthorn polyphenol-chitosan phospholipid complex is as follows: hawthorn polyphenol is dissolved in ethanol, chitosan and sodium tripolyphosphate solution are added, ionic cross-linking is carried out under pH 5.5 conditions, and then nanoparticles are obtained by high pressure homogenization. 2. Preparation process: The above active ingredients are mixed evenly, and soybean oil is added as a dispersion medium. The mixture is stirred to form a uniform suspension. The suspension is then fed into a soft capsule machine for pressing and shaping. After drying, quality inspection, and packaging, the anti-aging soft capsule product is obtained.

[0016] Example 2: Preparation of Anti-aging Solid Beverage 1. Raw material preparation (parts by weight): 10 parts ginsenosides, 12 parts total saponins from jujube seed, 5 parts rutin, 4 parts curcumin, 50 parts microcrystalline cellulose, and 2 parts steviol glycosides. 2. Preparation process: All powdered raw materials are passed through an 80-mesh sieve and then thoroughly mixed in a mixer. Granulation is performed using a dry granulation process, or the powder is directly filled into packets. The finished product is then packaged into strips to obtain the anti-aging solid beverage.

[0017] Experimental verification The efficacy was verified using a D-galactose-induced aging mouse model. Mice were administered the composition prepared in Example 1 of this invention by gavage for 4 consecutive weeks, and the following indicators were measured: serum NAD+ levels increased by approximately 38%; SIRT1 protein expression in liver tissue was significantly upregulated; mRNA levels of aging markers P16 and P21 were significantly reduced; mitochondrial complex I activity was restored, and reactive oxygen species (ROS) levels were significantly decreased; and the grip strength and voluntary movement ability of the mice were significantly better than those of the aging model control group, confirming that the composition of this invention has a clear anti-aging effect.

Claims

1. A composition for targeted multi-pathway synergistic anti-aging, characterized in that, Active ingredients include: Ginsenosides; Hawthorn polyphenol-chitosan phospholipid complex; Total saponins from jujube seed; Senolytics active ingredients are selected from at least one of quercetin, flavonoids, or fisetin; A natural mTOR inhibitor selected from at least one of resveratrol, curcumin, or rapamycin analogues.

2. The composition according to claim 1, characterized in that, The hawthorn polyphenol-chitosan phospholipid complex is a nanoscale complex formed by hawthorn polyphenol and chitosan under the action of a cross-linking agent, with a particle size range of 50-200 nm.

3. The composition according to claim 1, characterized in that, The weight ratio of each active ingredient in the composition is as follows: 5-20 parts ginsenosides, 10-30 parts hawthorn polyphenol-chitosan phospholipid complex, 5-20 parts total saponins of jujube seed, 3-10 parts senolytics active ingredient, and 3-10 parts natural mTOR inhibitor.

4. The composition according to claim 1, characterized in that, The composition can simultaneously activate Sirtuins protein activity, inhibit mTOR signaling pathway, activate mitophagy, inhibit CD38 enzyme activity, and clear senescent cells.

5. A health food product, characterized in that, It comprises the composition according to any one of claims 1-4, and food-grade excipients.

6. The health food according to claim 5, characterized in that, The dosage form of the health food is capsule, tablet, granule, powder or oral liquid.

7. The use of the composition according to any one of claims 1-4 in the preparation of products that delay physiological aging, improve metabolic function, or prolong healthy lifespan.

8. The application according to claim 7, characterized in that, The product is either a health food or a medicine.