Sewage denitrification recovery system and method based on Raman detection and quorum sensing quenching

Abstract

The invention provides a sewage denitrification recovery system and method based on Raman detection and quorum sensing quenching, and the system comprises a detection module which is used for detecting the metabolic impaired state of microorganisms in sewage, and the metabolic impaired state of the microorganisms is the metabolic impaired state of the microorganisms influenced by microplastics in the sewage; the analysis module is used for determining the influence degree of the water quality influenced by microbial metabolism by combining the microbial metabolism damaged state and the water quality state; and the quorum sensing quenching agent adding module is used for adding a quorum sensing quenching agent into the sewage according to the degree of influence of microbial metabolism on the water quality. The problem that the sewage treatment effect is reduced due to the fact that microplastics damage microbial metabolism is solved, and the stability of water quality is improved.

Description

Technical Field

[0001] This application relates to the field of wastewater denitrification technology, and in particular to a wastewater denitrification recovery system and method based on Raman detection and quorum induction quenching. Background Technology

[0002] Wastewater treatment plants are crucial facilities for urban nitrogen removal and environmental protection. Their core unit, denitrification, typically relies on the synergistic metabolic activity of nitrifying and denitrifying bacteria in activated sludge systems. However, in recent years, the continuous accumulation of microplastics in wastewater treatment systems has posed a potential threat to microbial ecological functions. Microplastics can induce excessive reactive oxygen species (ROS) in microorganisms, causing cell membrane damage and energy metabolism disorders, thereby disrupting the function of key denitrifying bacteria in wastewater and significantly reducing ammonia nitrogen removal rates. Summary of the Invention

[0003] In view of this, the purpose of this application is to propose a wastewater denitrification recovery system and method based on Raman detection and quorum sensing quenching, so as to solve the problem that microplastics damage microbial metabolism and thus reduce the wastewater treatment effect.

[0004] To achieve the above objectives, this application provides a wastewater denitrification recovery system based on Raman detection and quorum sensing quenching, comprising: The detection module is used to detect the metabolically damaged state of microorganisms in wastewater, wherein the metabolically damaged state of microorganisms is the metabolically damaged state of microorganisms affected by microplastics in wastewater; The analysis module is used to determine the extent to which water quality is affected by microbial metabolism by combining the state of microbial metabolic impairment and the state of water quality. A quorum quencher dosing module is used to add a quorum quencher to wastewater according to the degree to which the water quality is affected by microbial metabolism.

[0005] Optionally, a feedback module is also included, which is connected to the analysis module and is used to determine whether to control the quorum induction quencher dosing module to add the quorum induction quencher to the wastewater and to adjust the dosing amount based on the water quality health status after the quorum induction quencher dosing module adds the quorum induction quencher to the wastewater.

[0006] Optionally, it also includes a preprocessing module, the preprocessing module comprising: A filtration unit, wherein a filter screen is provided inside the filtration unit for filtering impurities in wastewater to obtain a microbial mixture; A centrifugation unit, connected to the filtration unit, is used to centrifuge the microbial mixture to obtain a microbial enrichment solution; A buffer conditioning unit is used to add buffer solution to the microbial enrichment solution to obtain a microbial detection solution. The detection module is connected to the buffer conditioning unit to detect the state of microbial metabolic impairment in the microbial detection solution.

[0007] Optionally, the quorum sensing quencher dosing module includes: Storage unit for storing quorum sensing quencher; The dosing unit, connected to the storage unit, is used to add quorum induction quencher to the wastewater.

[0008] Optionally, the dosing unit is connected to the feedback module and is used to receive the dosing amount instruction from the feedback module and add a corresponding amount of quorum induction quencher to the wastewater according to the instruction.

[0009] Optionally, a control module is also included, which is connected to the detection module, the analysis module and the quorum induction quencher dosing module respectively, and is used to control the operation of the detection module, the analysis module and the quorum induction quencher dosing module respectively.

[0010] Based on the same inventive concept, this disclosure also provides a wastewater denitrification recovery method based on Raman detection and quorum sensing quenching, using the system described in any of the above claims, including the following steps: Detect the metabolic impairment state of microorganisms in wastewater due to the influence of microplastics; Based on the metabolic impairment state of microorganisms affected by microplastics, and in combination with the water quality status of the wastewater, the degree to which the wastewater quality is affected by microbial metabolism is determined. Based on the degree to which wastewater quality is affected by microbial metabolism, a quorum sensing quencher is added to the wastewater.

[0011] Optionally, the detection of the metabolically impaired state of microorganisms in wastewater due to microplastics includes: Impurities in the wastewater are filtered out to obtain a microbial mixture; The microbial mixture was centrifuged to obtain a microbial enrichment solution; Add buffer solution to the microbial enrichment solution and adjust the pH value to obtain the microbial detection solution; To detect the metabolically impaired state of microorganisms in microbial test solutions due to the influence of microplastics.

[0012] Optional, also includes: In response to the determination to add a quorum induction quencher to the wastewater, the water quality health status is detected at preset intervals. If the current water quality health status of the wastewater does not meet the preset recovery standard, the quorum sensing quencher dosing module will be controlled again to add quorum sensing quencher until the water quality health status of the wastewater reaches the preset recovery standard.

[0013] Based on the same inventive concept, this disclosure also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and executable by the processor, wherein the processor implements the method described above when executing the computer program.

[0014] As can be seen from the above, the wastewater denitrification recovery system based on Raman detection and quorum sensing quenching provided in this application includes: a detection module, an analysis module, and a quorum sensing quencher dosing module. The detection module can accurately capture the metabolic damage state of microorganisms affected by microplastics in wastewater, providing targeted data support for subsequent analysis. The analysis module, based on the metabolic damage state and water quality status, determines whether the water quality is affected by microbial metabolic damage and the specific degree of impact: if microbial metabolism is severely damaged and the water quality is extremely poor, it is determined that the water quality is significantly affected by microbial metabolism. At this time, the destructive effect of microplastics in wastewater on microbial metabolism is significant, leading to severe disorder of the denitrifying bacterial community, which in turn causes a significant decrease in ammonia nitrogen removal rate and obvious deterioration of water quality. If microbial metabolism is only slightly damaged and the water quality is only slightly exceeded, it is determined that the water quality is less affected by microbial metabolism. If microbial metabolism is not damaged but the water quality is poor, it is determined that microbial metabolic damage has almost no impact on water quality, indicating that water quality deterioration is more likely caused by non-microbial metabolic factors. The quorum sensing quencher dosing module precisely controls the dosage of the quencher based on the degree to which water quality is affected by microbial metabolism. When the impact is significant, the dosage is increased accordingly. After dosing, the quencher effectively blocks the abnormal quorum sensing process induced by microplastics, reduces the excessive accumulation of reactive oxygen species (ROS) in microbial cells, alleviates oxidative damage to cell membranes, and gradually restores the activity of key denitrification enzymes (such as ammonia monooxygenase and nitrate reductase). As microbial metabolic function gradually recovers, the removal efficiency of ammonia nitrogen and nitrite in wastewater steadily improves, and related indicators continuously approach discharge standards, ultimately achieving effective water quality restoration. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in this application or related technologies, the drawings used in the description of the embodiments or related technologies will be briefly introduced below. Obviously, the drawings described below are only embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of a wastewater denitrification recovery system based on Raman detection and quorum induction quenching, as shown in the embodiments of this application. Figure 2 This is a schematic diagram illustrating a wastewater denitrification recovery method based on Raman detection and quorum sensing quenching, as shown in an embodiment of this application. Figure 3 A structural diagram of the electronic hardware is shown for an embodiment of this application. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with specific embodiments and the accompanying drawings.

[0018] It should be noted that, unless otherwise defined, the technical or scientific terms used in the embodiments of this application should have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms "first," "second," and similar terms used in the embodiments of this application do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed after the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are only used to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0019] As mentioned in the background, the denitrification system of wastewater treatment plants relies on the synergistic metabolism of nitrifying and denitrifying bacteria in activated sludge to convert nitrogen into nitrogen gas through a series of reactions. Microplastics, as an emerging persistent pollutant, pose a significant threat to this system. Microplastics mainly enter the treatment system through domestic sewage, industrial wastewater, and atmospheric deposition. Due to their strong physicochemical stability, their removal effect in pretreatment units such as screens and grit chambers is limited. The remaining microplastics combine with activated sludge flocs and continue to accumulate in core treatment units such as aeration tanks and secondary sedimentation tanks. Moreover, with the increase in plastic production, their accumulation trend within the system is on the rise. The stress of microplastics on denitrification systems manifests in multiple dimensions: From a physical perspective, the hydrophobic properties of microplastic surfaces make them prone to cross-linking with sludge flocs to form a dense barrier layer, hindering the normal transfer of oxygen to the substrate. At high concentrations, they can also damage the structure of sludge flocs, leading to floc breakage, deterioration of settling performance, and further exacerbating the problem of uneven mass transfer. From a chemical toxicity perspective, microplastics can not only accumulate pollutants such as heavy metals, antibiotics, and polycyclic aromatic hydrocarbons through surface adsorption, forming more toxic complex systems, but also release their own plasticizers, stabilizers, and other additives slowly into the aquatic environment, directly inhibiting microbial enzyme activity. At the same time, microplastic exposure can induce excessive reactive oxygen species in microbial cells, triggering oxidative stress, damaging cell membrane integrity, interfering with energy metabolism, and damaging the normal physiological functions of the microbial community. From a microbial ecology perspective, microplastics can lead to a decrease in the abundance of key denitrifying bacteria, downregulation of functional gene transcription levels, and disruption of the metabolic synergy between nitrifying and denitrifying bacteria, potentially causing problems such as nitrite accumulation or incomplete ammonia nitrogen treatment, thus weakening the overall operational efficiency of the denitrification metabolic pathway. When a denitrification system encounters a short-term, high-intensity microplastic shock, it exhibits characteristics of sudden functional collapse. Ammonia nitrogen removal capacity drops sharply, effluent nitrogen levels easily exceed standards, sludge activity decreases significantly, extracellular polymer secretion becomes disordered, and the system loses its own shock resistance. The recovery process after such a shock is often quite difficult. On the one hand, the key denitrifying bacteria have a long proliferation cycle, making it difficult to restore their abundance and activity to pre-shock levels in a short period. On the other hand, the microplastics introduced during the shock cannot be effectively removed through the system's own metabolism, continuously stressing the bacteria and significantly prolonging the recovery period.

[0020] In summary, microplastics exert comprehensive stress on denitrification systems through multiple pathways, including physical barriers, chemical toxicity, and microbial dysbiosis. This is a key factor leading to a decline in the system's ammonia nitrogen removal efficiency. In particular, short-term high-intensity impacts pose a severe challenge to the stable operation of wastewater treatment plants, necessitating the establishment of a targeted early warning and prevention system.

[0021] To address the aforementioned issues, this application provides a wastewater denitrification recovery system and method based on Raman detection and quorum induction quenching.

[0022] The following is in conjunction with the appendix Figure 1-3 The embodiments of this application will be described in detail below.

[0023] A wastewater denitrification recovery system based on Raman detection and quorum sensing quenching includes: The detection module is used to detect the metabolically damaged state of microorganisms in wastewater, wherein the metabolically damaged state of microorganisms is the metabolically damaged state of microorganisms affected by microplastics in wastewater; The analysis module is used to determine the extent to which water quality is affected by microbial metabolism by combining the state of microbial metabolic impairment and the state of water quality. A quorum quencher dosing module is used to add a quorum quencher to wastewater according to the degree to which the water quality is affected by microbial metabolism.

[0024] Specifically, the detection module can be a single-cell Raman spectroscopy detection module, which samples and analyzes microorganisms in wastewater samples. Raman spectral signals can reflect the metabolic activity and physiological state of microorganisms, such as changes in energy metabolites, proteins, and lipids, and can be used to identify the degree of damage to key functional bacteria (such as ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and denitrifying bacteria). The detection frequency and sampling points can be set according to the system scale and operational requirements.

[0025] Furthermore, for example, the single-cell Raman spectroscopy detection module can determine the metabolic damage status of microorganisms by detecting the content of reactive oxygen species (ROS) and the activity of key denitrification enzymes (such as ammonia monooxygenase and nitrate reductase). If the ROS content is higher than the normal threshold or the denitrification enzyme activity is lower than the normal threshold, it is determined that the microorganisms have suffered metabolic damage due to the influence of microplastics.

[0026] The core processing unit of the analysis module can be a Siemens S7-1200 series PLC adapted for industrial wastewater treatment scenarios. It receives metabolic damage data from the detection module and collects water quality parameters such as ammonia nitrogen concentration, nitrite concentration, and dissolved oxygen in the wastewater through the water quality acquisition module. This data is then combined for comprehensive analysis to determine the degree to which water quality is affected by microbial metabolism. When the accumulated ROS exceeds the normal metabolic threshold for microorganisms and the denitrification enzyme activity is below the normal functional threshold, microbial metabolism is considered damaged (the degree of damage is categorized as "severe," "moderate," or "slight"; severe damage is indicated by significantly exceeding the ROS threshold and a large decrease in enzyme activity, while slight damage is indicated by less ROS and less enzyme activity). When the ammonia nitrogen concentration and nitrite concentration exceed the wastewater discharge standards, the water quality is considered poor (the degree of poorness is categorized as "extremely poor," "moderately poor," or "slightly poor"; significant exceedances result in extremely poor water quality, while minor exceedances result in slightly poor water quality). Based on this, a gradient judgment is formed: if microbial metabolism is severely damaged and water quality is extremely poor, it is determined that the water quality is greatly affected by microbial metabolism. This indicates that microplastics in wastewater significantly damage microbial metabolism, leading to severe disorder of denitrifying bacteria, a significant decrease in ammonia nitrogen removal rate, and obvious water quality deterioration. If microbial metabolism is slightly damaged and water quality is slightly poor, it is determined that the water quality is less affected by microbial metabolism. If microbial metabolism is not damaged but water quality is poor, it is determined that microbial metabolic damage has almost no impact on water quality, indicating that water quality deterioration is more likely caused by non-microbial metabolic factors.

[0027] The quorum quencher dosing module adds a quorum quencher to the wastewater based on the degree to which the water quality is affected by microbial metabolism. When the degree of influence on water quality by microbial metabolism is significant, a larger amount of quorum quencher is added. The quorum quencher can be a chemical substance (e.g., furanone derivatives, γ-caprolactone), an enzyme (e.g., lactonease, acyltransferase), or a microorganism with quenching activity (e.g., Rhodococcus sp.). Dosing methods may include solution injection, release via immobilized carrier, or slow release via microspheres. After addition, the quorum quencher can block abnormal quorum sensing induced by microplastics, reduce the accumulation of reactive oxygen species (ROS), alleviate cell membrane damage, and restore the activity of key denitrifying enzymes (e.g., ammonia monooxygenase, nitrate reductase). As the metabolic function of microorganisms gradually recovers, the removal efficiency of ammonia nitrogen and nitrite in the wastewater improves, and the indicators gradually approach the discharge standards, ultimately achieving effective water quality restoration.

[0028] In this embodiment, the detection module can accurately capture the metabolic damage state of microorganisms affected by microplastics in wastewater, providing targeted data support for subsequent analysis. The analysis module, based on this metabolic damage state and water quality status, determines in a gradient whether the water quality is affected by microbial metabolic damage and the specific degree of impact: if microbial metabolism is severely damaged and water quality is extremely poor, the degree of impact on water quality from microbial metabolism is determined to be large. At this time, the destructive effect of microplastics in wastewater on microbial metabolism is significant, leading to severe disorder of denitrifying bacteria, resulting in a significant decrease in ammonia nitrogen removal rate and obvious deterioration of water quality. If microbial metabolism is only slightly damaged and water quality is only slightly exceeded, the degree of impact on water quality from microbial metabolism is determined to be small. If microbial metabolism is not damaged but water quality is poor, the degree of impact on water quality from microbial metabolism is determined to be almost negligible, indicating that water quality deterioration is more likely caused by non-microbial metabolic factors (such as physical impurity accumulation, external pollutant impact, etc.). The quorum sensing quencher dosing module precisely controls the dosage of quorum sensing quencher according to the degree of impact on water quality from microbial metabolism. When the impact is significant, the dosage is increased accordingly. After the addition of quorum sensing quenchers, the abnormal quorum sensing process induced by microplastics can be effectively blocked, reducing the excessive accumulation of reactive oxygen species (ROS) in microbial cells, alleviating oxidative damage to cell membranes, and gradually restoring the activity of key denitrification enzymes (such as ammonia monooxygenase and nitrate reductase). As the metabolic functions of microorganisms gradually recover, the removal efficiency of ammonia nitrogen and nitrite in wastewater steadily improves, and related indicators continue to approach the discharge standards, ultimately achieving effective water quality restoration.

[0029] In some embodiments, the wastewater denitrification recovery system based on Raman detection and quorum induction quenching further includes a feedback module connected to the analysis module. The feedback module is used to determine whether to control the quorum induction quenching agent dosing module to add the quorum induction quenching agent to the wastewater and to adjust the dosage based on the water quality health status after the quorum induction quenching agent dosing module adds the quorum induction quenching agent to the wastewater.

[0030] Specifically, this feedback module uses an embedded microcontroller (such as the STM32F4 series) as its core control unit and is essentially a control submodule. One end of the feedback module is stably connected to the analysis module, and the other end is connected to the quorum induction quencher dosing module via a signal line. After the quorum induction quencher dosing module completes the first round of quorum induction quencher dosing, the feedback module will trigger a preset monitoring cycle (e.g., every 3-4 hours) to receive the water quality health status data generated by the analysis module in real time. This data includes water quality indicators such as ammonia nitrogen concentration and nitrite concentration. The feedback module compares the current water quality health status with the baseline status before dosing: if the water quality health status has improved from slightly or severely damaged to normal (water quality indicators meet standards), it immediately sends a stop dosing command to the dosing module; if the water quality health status has improved but not reached normal, it calculates and sends a reduction dosing command based on the improvement (e.g., reducing the original dosing by 30%-50%); if the water quality health status shows no significant change or even deteriorates, the feedback module determines that the current quencher dosage is insufficient or that other interfering factors exist. It sends an incremental dosing command to the dosing module and simultaneously synchronizes the abnormal situation to the system alarm unit, alerting staff to check for secondary microplastic impact or equipment malfunction. This dynamic feedback adjustment prevents the stagnation of denitrification recovery due to insufficient quorum sensing quencher dosage, thus significantly improving the system's response flexibility and recovery efficiency to water quality changes.

[0031] In this embodiment, after the quorum induction quencher dosing module adds the quorum induction quencher to the wastewater, the feedback module determines whether to control the quorum induction quencher dosing module to add the quencher again and adjust the dosage based on the water quality health status. This can prevent the denitrification function of the water body from stagnating due to insufficient quorum induction quencher dosage, thereby significantly improving the system's response flexibility and recovery efficiency to water quality changes.

[0032] In some embodiments, the wastewater denitrification recovery system based on Raman detection and quorum sensing quenching further includes a pretreatment module, the pretreatment module comprising: A filtration unit, wherein a filter screen is provided inside the filtration unit for filtering impurities in wastewater to obtain a microbial mixture; A centrifugation unit, connected to the filtration unit, is used to centrifuge the microbial mixture to obtain a microbial enrichment solution; A buffer conditioning unit is used to add buffer solution to the microbial enrichment solution to obtain a microbial detection solution. The detection module is connected to the buffer conditioning unit to detect the state of microbial metabolic impairment in the microbial detection solution.

[0033] Specifically, the filtration unit has a built-in corrosion-resistant filter screen with a pore size of 1-5 microns (materials can be polypropylene or stainless steel). After the wastewater enters the filtration unit, the filter screen can efficiently trap silt, large organic debris, and large microplastic particles in the water, preventing impurities from encapsulating microorganisms or interfering with subsequent detection reactions, ultimately obtaining a microbial mixture with microorganisms as the main component. The centrifugation unit is connected to the outlet of the filtration unit through a corrosion-resistant pipe. After receiving the microbial mixture, it centrifuges the mixture to enrich the microorganisms and allow them to settle to the bottom of the centrifuge tube. Then, the supernatant containing soluble impurities is removed through an automatic drain valve to obtain the microbial enrichment solution. The buffer solution adjustment unit injects buffer solution (preset pH) into the microbial enrichment solution through a metered pump. With a pH of 7.0-7.5 (matching the suitable living environment for microorganisms), the buffer solution can neutralize any acid-base fluctuations in the enrichment solution, maintain stable osmotic pressure, and prevent additional metabolic damage to microorganisms due to sudden environmental changes. Ultimately, it forms a microbial detection solution with uniform composition and stable microbial activity. The detection module is connected to the outlet of the buffer solution adjustment unit through a dedicated sampling pipe, allowing direct extraction of the detection solution to accurately detect metabolic damage indicators such as the cumulative ROS of microorganisms and the activity of key denitrification enzymes. This effectively avoids interference from impurities in the original wastewater and environmental fluctuations on the detection results.

[0034] In this embodiment, the pretreatment module includes a filtration unit, a centrifugation unit, and a buffer conditioning unit. The filter screen in the filtration unit can effectively intercept large particulate impurities (such as silt, organic debris, etc.) in the wastewater, preventing these impurities from mixing into subsequent detection stages. This prevents impurities from clogging the sampling channels or detection elements of the detection module and also avoids impurities interfering with the capture of microbial metabolic signals, thereby obtaining a microbial mixture with higher purity. The centrifugation unit is connected to the filtration unit and enriches the microorganisms in the microbial mixture through centrifugation to obtain a microbial enrichment solution. This process can increase the concentration of microorganisms in the detection solution, avoiding the problem of weak detection signals and large errors due to low microbial content, and improving the sensitivity of subsequent detection. The buffer conditioning unit adds buffer solution to the microbial enrichment solution to maintain a stable pH and osmotic pressure of the system, avoiding drastic changes in the enrichment solution environment that could lead to abnormal microbial activity. This ensures that the microbial state in the microbial detection solution is consistent with the actual metabolically damaged state in the wastewater, providing the detection module with a real and reliable detection sample.

[0035] In some embodiments, the quorum quencher dosing module includes: Storage unit for storing quorum sensing quencher; The dosing unit, connected to the storage unit, is used to add quorum induction quencher to the wastewater.

[0036] In addition, the dosing unit is connected to the feedback module and is used to receive the dosing amount instruction from the feedback module and add the corresponding amount of quorum induction quencher to the wastewater according to the instruction.

[0037] Specifically, the storage unit of the quorum induction quencher dosing module uses a sealed storage tank made of 304 stainless steel. The inner wall of the tank is treated with anti-corrosion to adapt to the storage requirements of different types of quenchers. The tank is also equipped with a low-speed stirrer to prevent sedimentation and stratification when the quencher is left to stand. It is also equipped with a temperature control component to avoid temperature fluctuations affecting the activity of the quencher. A liquid level sensor is installed at the bottom of the storage tank to monitor the remaining amount of quencher in real time and feed it back to the main control interface of the system.

[0038] The dosing unit is connected to the outlet of the storage unit via a corrosion-resistant polyethylene pipe. A solenoid valve on the pipe controls the material flow. The main body of the dosing unit is a high-precision metering pump, with its outlet connected to multiple dosing ports in the wastewater treatment tank to ensure uniform diffusion of the quenching agent into the wastewater. Furthermore, the control unit of the dosing unit establishes a data connection with the feedback module via a communication protocol. When the feedback module generates a dosing command (including the specific dosage of the quenching agent based on the water quality status, such as 50 mg / L or 100 mg / L) based on the quorum sensing agent dosage, the command is transmitted to the controller of the dosing unit in the form of an electrical signal. The controller then adjusts the speed and running time of the metering pump to ensure the quenching agent dosage precisely matches the command requirements. If the feedback module determines that the water quality has improved and the dosing needs to be reduced or stopped, the dosing unit can also respond in real time, adjusting the output of the metering pump or closing the solenoid valve to achieve dynamic and precise dosing of the quenching agent.

[0039] In this embodiment, the storage unit of the quorum sensing quencher dosing module provides a stable storage environment for the quencher, preventing degradation or ineffectiveness due to improper storage and ensuring the continuity and effectiveness of the agent supply. The dosing unit is connected to the storage unit to achieve stable reception of the quorum sensing quencher. Simultaneously, the connection design between the dosing unit and the feedback module allows it to receive dosing instructions generated in real time by the feedback module based on the degree of influence of microbial metabolism on water quality. This enables precise control of the quencher dosing amount, avoiding both insufficient dosing leading to poor blocking effect against abnormal microbial quorum sensing and excessive dosing causing agent waste or unnecessary interference with normal microbial communities in wastewater. In some embodiments, the wastewater denitrification recovery system based on Raman detection and quorum induction quenching further includes a control module, which is connected to the detection module, the analysis module, and the quorum induction quenching agent dosing module, respectively, and is used to control the operation of the detection module, the analysis module, and the quorum induction quenching agent dosing module.

[0040] For example, the control module can be a Siemens S7-1200 series PLC. The control module is also equipped with an industrial touch screen as a human-machine interface device to display the operating status of each module in real time (such as the sampling frequency of the detection module, the judgment result of the analysis module, and the remaining amount of reagent in the dosing module). At the same time, it supports operators to manually set control parameters (such as the detection interval duration and the dosing threshold).

[0041] Specifically, the control module establishes bidirectional communication with the detection module, analysis module, and quorum induction quencher dosing module, ensuring coordinated operation of each module through timing control and command interaction. Specifically, for the detection module, the control module can preset the detection cycle, such as starting detection every 30 minutes, periodically sending data acquisition commands to the detection module, and simultaneously receiving raw data such as ROS content and denitrification enzyme activity from the detection module. If the detection module malfunctions, the control module will immediately send a pause command and trigger a local audible and visual alarm. For the analysis module, after receiving data from the detection module, the control module sends data processing commands to the analysis module, controlling the analysis module to start the calculation process, and simultaneously receiving the results of the analysis module's output regarding the degree of water quality affected by microbial metabolism. For the quorum induction quencher dosing module, the control module converts the analysis module's judgment results into equipment action commands; for example, if it determines that the water quality is significantly affected by microbial metabolism, it sends a dosing command to the dosing unit.

[0042] In this embodiment, the control module is connected to the detection module, analysis module, and quorum sensing quencher dosing module respectively, realizing unified control over the operation of these three modules. It can schedule the detection module to carry out timely detection of microbial metabolic damage, trigger the analysis module to determine the degree of water quality affected by microbial metabolism based on the detection data, and control the quencher dosing action of the dosing module according to the analysis results. This avoids the disconnection of the operation of each module, ensures the orderly and continuous flow of wastewater denitrification recovery process, and ensures the stable performance of core functions.

[0043] Based on the same inventive concept, such as Figure 2 As shown, this application also discloses a wastewater denitrification recovery method based on Raman detection and quorum sensing quenching, which utilizes the system described in any of the above claims and includes the following steps: S100: Detects the metabolic impairment state of microorganisms in wastewater due to the influence of microplastics; In this step, the detection module can be a single-cell Raman spectroscopy detection module. This module obtains a water sample containing microorganisms from the wastewater to be treated via a sampling component. Then, the built-in detection elements are used to measure changes in microbial energy metabolites, proteins, and lipids. For example, the metabolic damage status of the microorganisms can be determined by detecting the content of reactive oxygen species (ROS) and the activity of key denitrification enzymes (such as ammonia monooxygenase and nitrate reductase). If the ROS content is higher than the normal threshold or the denitrification enzyme activity is lower than the normal threshold, it is determined that the microorganisms have suffered metabolic damage due to the influence of microplastics.

[0044] S200: Determine the degree to which wastewater quality is affected by microbial metabolism based on the metabolic impairment state of microorganisms affected by microplastics and in combination with the wastewater quality status. In this step, the analysis module first receives the metabolic impairment data output by the detection module in step S100, and simultaneously acquires the wastewater quality parameters through the water quality acquisition module, specifically including ammonia nitrogen concentration (which can be detected using Nessler's reagent spectrophotometry), nitrite concentration (which can be detected using diazo coupling spectrophotometry), and dissolved oxygen content (which can be acquired in real time using a dissolved oxygen sensor). The analysis module combines the above data for comprehensive analysis to determine the degree to which water quality is affected by microbial metabolism. For example, if the cumulative amount of microbial ROS exceeds the benchmark value by more than 30% and the denitrification enzyme activity is more than 50% below the standard threshold (indicating severe metabolic damage), while the concentrations of ammonia nitrogen and nitrite exceed the wastewater discharge standard by more than 1 time (indicating extremely poor water quality), then the water quality is considered to be significantly affected by microbial metabolism. This indicates that microplastics in the wastewater significantly damage microbial metabolism, leading to severe dysfunction of the denitrifying bacterial community, a significant decrease in ammonia nitrogen removal rate, and obvious water quality deterioration. If the cumulative amount of microbial ROS exceeds the benchmark value by 10%-30% and the denitrification enzyme activity is 20%-50% below the standard threshold (indicating slight metabolic damage), while the concentrations of ammonia nitrogen and nitrite exceed the discharge standard by 0.2-1 time (indicating slightly poor water quality), then the water quality is considered to be less affected by microbial metabolism. If the microbial metabolic indicators are normal (indicating no metabolic damage), but the water quality parameters exceed the standard, then the water quality is considered to be unaffected by microbial metabolism, indicating that water quality deterioration is more likely caused by non-microbial metabolic factors.

[0045] S300: Based on the degree to which wastewater quality is affected by microbial metabolism, add a quorum sensing quencher to the wastewater.

[0046] In this step, the quorum sensing quencher dosing module of the system is implemented. The dosing module first receives the impact degree judgment result output by the analysis module in step S200: if the water quality is greatly affected by microbial metabolism, the storage unit delivers a larger amount of quencher to the dosing unit; if the water quality is less affected by microbial metabolism, a smaller amount of quencher is delivered to the dosing unit; if the water quality is not affected by microbial metabolism, the dosing unit does not initiate the dosing action. During the dosing process, the dosing unit monitors the quencher dosing flow rate in real time to ensure that the dosing amount matches the judgment result, so as to accurately block the impact of microplastics on microbial metabolism.

[0047] The method in this embodiment has the same effect as any of the system embodiments described above, and will not be repeated here.

[0048] In some embodiments, step S100, detecting the metabolically impaired state of microorganisms in wastewater due to microplastics, includes: S101: Filter out impurities from wastewater to obtain a microbial mixed liquor; In this step, after the wastewater enters the filtration unit, it passes through the filter screen. The filter screen can efficiently trap silt, large organic debris, and large microplastic particles in the water, preventing impurities from encapsulating microorganisms or interfering with subsequent detection reactions, and finally obtaining a microbial mixture with microorganisms as the main component.

[0049] S102: Centrifuge the microbial mixture to obtain a microbial enrichment solution; In this step, the obtained microbial mixture is transferred to a centrifuge tube and centrifuged in a benchtop high-speed centrifuge (such as the Xiangyi TG16-WS model) to allow the microbial cells to settle to the bottom of the centrifuge tube. The upper clear liquid is then discarded, and the microbial precipitate at the bottom is retained to obtain a microbial enrichment solution with a higher concentration, thereby improving the sensitivity of subsequent detection. S103: Add buffer solution to the microbial enrichment solution and adjust the pH value to obtain the microbial detection solution; In this step, PBS buffer (phosphate buffered salt solution) is slowly added to the microbial enrichment solution while gently mixing. At the same time, the pH value of the mixture is monitored in real time using a portable pH meter (such as Leici PHS-3C model). The pH is adjusted to the range of 7.0-7.5, which is close to the acid-base environment in which microorganisms naturally survive. This avoids the additional impact of pH abnormalities on the metabolic state of microorganisms, and finally obtains a microbial detection solution suitable for detection.

[0050] S104: Detects the metabolically impaired state of microorganisms in microbial test solutions due to the influence of microplastics.

[0051] In this embodiment, step S101 filters wastewater impurities to remove interfering substances such as silt and suspended particles, preventing them from affecting subsequent microbial separation and detection; step S102 centrifuges the microbial mixture to rapidly concentrate microorganisms, increase the concentration of target bacteria in the microbial enrichment solution, and enhance the stability of subsequent detection signals; step S103 adds buffer solution and adjusts the pH value to the microbial enrichment solution to control the pH of the microbial enrichment solution within a suitable range for microbial survival and metabolic activity, avoiding fluctuations in microbial activity caused by abnormal pH, ensuring that the microbial state during detection closely matches the metabolic state in actual wastewater, so as to accurately obtain data on the metabolic damage state of microorganisms affected by microplastics, and ensure the accuracy and reliability of the detection results.

[0052] In some embodiments, the wastewater denitrification recovery method based on Raman detection and quorum sensing quenching further includes: S400: In response to the determination to add quorum induction quencher to wastewater, the water quality health status is detected at preset intervals; S500: In response to the current water quality health status of the wastewater not reaching the preset recovery standard, the quorum sensing quencher dosing module will be controlled again to add quorum sensing quencher until the water quality health status of the wastewater reaches the preset recovery standard.

[0053] Specifically, the analysis module first compares the current water quality health status indicators with the preset recovery standards (such as ammonia nitrogen ≤ 5 mg / L, nitrite ≤ 0.5 mg / L, and dissolved oxygen maintained at 2-4 mg / L). If it determines that the standards are not met, it immediately sends a signal to the control module. After receiving the signal, the control module adjusts the dosing parameters based on the correlation data between the current water quality and the previous dosage. For example, if the indicators did not meet the standards but improved after the previous dosage of 1 mg / L, the dosage can be adjusted to 0.5-0.8 mg / L, and then the dosing unit of the quorum sensing quencher dosing module is controlled to start the second dosing. After the dosing is completed, the water quality test is repeated at the interval set by S400 until the analysis module determines that the wastewater quality health status has reached the preset recovery standards, and then the subsequent dosing action is stopped.

[0054] In this embodiment, step S400 monitors the water quality status at preset intervals after adding the quorum sensing quencher, enabling real-time tracking of water quality changes after quencher addition and preventing delays in monitoring the recovery process due to a lack of dynamic monitoring. Step S500, in case the water quality fails to meet the preset recovery standard, promptly controls the dosing module to add quencher again, effectively compensating for potential insufficient dosage in a single application and preventing stagnation in water quality recovery. The combined action of these two steps ensures that the wastewater quality gradually improves under the continuous action of the quencher until it stably reaches the preset recovery standard, ensuring the effectiveness and compliance rate of wastewater denitrification recovery.

[0055] It should be noted that the method in this embodiment can be executed by a single device, such as a computer or server. The method can also be applied in a distributed scenario, where multiple devices cooperate to complete the process. In such a distributed scenario, one of these devices may execute only one or more steps of the method in this embodiment, and the devices will interact with each other to complete the method described.

[0056] It should be noted that the above description describes some embodiments of this application. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps described in the claims can be performed in a different order than that shown in the above embodiments and still achieve the desired result. Furthermore, the processes depicted in the drawings do not necessarily require a specific or sequential order to achieve the desired result. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.

[0057] Based on the same inventive concept, corresponding to the methods of any of the above embodiments, this application also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the program, it implements the wastewater denitrification recovery method based on Raman detection and quorum induction quenching as described in any of the above embodiments.

[0058] Figure 3 This embodiment illustrates a more specific hardware structure of an electronic device. The device may include a processor 1010, a memory 1020, an input / output interface 1030, a communication interface 1040, and a bus 1050. The processor 1010, memory 1020, input / output interface 1030, and communication interface 1040 are interconnected internally via the bus 1050.

[0059] The processor 1010 can be implemented using a general-purpose CPU (Central Processing Unit), microprocessor, application-specific integrated circuit (ASIC), or one or more integrated circuits, and is used to execute relevant programs to implement the technical solutions provided in the embodiments of this specification.

[0060] The memory 1020 can be implemented in the form of ROM (Read Only Memory), RAM (Random Access Memory), static storage device, dynamic storage device, etc. The memory 1020 can store the operating system and other applications. When the technical solutions provided in the embodiments of this specification are implemented by software or firmware, the relevant program code is stored in the memory 1020 and is called and executed by the processor 1010.

[0061] The input / output interface 1030 is used to connect input / output modules to realize information input and output. Input / output modules can be configured as components within the device (not shown in the figure) or externally connected to the device to provide corresponding functions. Input devices may include keyboards, mice, touchscreens, microphones, various sensors, etc., while output devices may include displays, speakers, vibrators, indicator lights, etc.

[0062] The communication interface 1040 is used to connect a communication module (not shown in the figure) to enable communication between this device and other devices. The communication module can communicate via wired means (such as USB, Ethernet cable, etc.) or wireless means (such as mobile network, WIFI, Bluetooth, etc.).

[0063] Bus 1050 includes a pathway for transmitting information between various components of the device, such as processor 1010, memory 1020, input / output interface 1030, and communication interface 1040.

[0064] It should be noted that although the above-described device only shows the processor 1010, memory 1020, input / output interface 1030, communication interface 1040, and bus 1050, in specific implementations, the device may also include other components necessary for normal operation. Furthermore, those skilled in the art will understand that the above-described device may only include the components necessary for implementing the embodiments of this specification, and not necessarily all the components shown in the figures.

[0065] The electronic devices described in the above embodiments are used to implement the wastewater denitrification recovery method based on Raman detection and quorum induction quenching in any of the foregoing embodiments, and have the beneficial effects of the corresponding method embodiments, which will not be repeated here.

[0066] Based on the same inventive concept, corresponding to the methods of any of the above embodiments, this application also provides a non-transitory computer-readable storage medium storing computer instructions for causing the computer to execute the wastewater denitrification recovery method based on Raman detection and quorum induction quenching as described in any of the above embodiments.

[0067] The computer-readable medium of this embodiment includes permanent and non-permanent, removable and non-removable media, and information storage can be implemented by any method or technology. Information can be computer-readable instructions, data structures, program modules, or other data. Examples of computer storage media include, but are not limited to, phase-change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory or other memory technologies, CD-ROM, digital versatile optical disc (DVD) or other optical storage, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other non-transfer medium that can be used to store information accessible by a computing device.

[0068] The computer instructions stored in the storage medium of the above embodiments are used to cause the computer to execute the wastewater denitrification recovery method based on Raman detection and quorum induction quenching as described in any of the above embodiments, and have the beneficial effects of the corresponding method embodiments, which will not be repeated here.

[0069] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of this application is limited to these examples; under the concept of this application, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of different aspects of the embodiments of this application as described above, which are not provided in detail for the sake of brevity.

[0070] Additionally, to simplify the description and discussion, and to avoid obscuring the embodiments of this application, the well-known power / ground connections to integrated circuit (IC) chips and other components may or may not be shown in the provided drawings. Furthermore, the apparatus may be shown in block diagram form to avoid obscuring the embodiments of this application, and this also takes into account the fact that the details of the implementation of these block diagram apparatuses are highly dependent on the platform on which the embodiments of this application will be implemented (i.e., these details should be fully understood by those skilled in the art). While specific details (e.g., circuits) have been set forth to describe exemplary embodiments of this application, it will be apparent to those skilled in the art that the embodiments of this application can be implemented without these specific details or with variations thereof. Therefore, these descriptions should be considered illustrative rather than restrictive.

[0071] Although this application has been described in conjunction with specific embodiments thereof, many substitutions, modifications, and variations of these embodiments will be apparent to those skilled in the art from the foregoing description. For example, other memory architectures (e.g., dynamic RAM (DRAM)) may be used with the embodiments discussed.

[0072] The embodiments of this application are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the claims of this application. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the embodiments of this application should be included within the protection scope of this application.

Claims

1. A wastewater denitrification recovery system based on Raman detection and quorum sensing quenching, characterized in that, include: The detection module is used to detect the metabolically damaged state of microorganisms in wastewater, wherein the metabolically damaged state of microorganisms is the metabolically damaged state of microorganisms affected by microplastics in wastewater; The analysis module is used to determine the extent to which water quality is affected by microbial metabolism by combining the state of microbial metabolic impairment and the state of water quality. A quorum quencher dosing module is used to add a quorum quencher to wastewater according to the degree to which the water quality is affected by microbial metabolism.

2. The system according to claim 1, characterized in that, It also includes a feedback module, which is connected to the analysis module. The feedback module is used to determine whether to control the quorum induction quencher dosing module to add the quorum induction quencher to the wastewater and to adjust the dosing amount based on the water quality health status after the quorum induction quencher dosing module adds the quorum induction quencher to the wastewater.

3. The system according to claim 1, characterized in that, It also includes a preprocessing module, which includes: A filtration unit, wherein a filter screen is provided inside the filtration unit for filtering impurities in wastewater to obtain a microbial mixture; A centrifugation unit, connected to the filtration unit, is used to centrifuge the microbial mixture to obtain a microbial enrichment solution; A buffer conditioning unit is used to add buffer solution to the microbial enrichment solution to obtain a microbial detection solution. The detection module is connected to the buffer conditioning unit to detect the state of microbial metabolic impairment in the microbial detection solution.

4. The system according to claim 2, characterized in that, The quorum sensing quencher dosing module includes: Storage unit for storing quorum sensing quencher; The dosing unit, connected to the storage unit, is used to add quorum induction quencher to the wastewater.

5. The system according to claim 4, characterized in that, The dosing unit is connected to the feedback module and is used to receive the dosing amount instruction from the feedback module and add the corresponding amount of quorum induction quencher to the wastewater according to the instruction.

6. The system according to claim 1, characterized in that, It also includes a control module, which is connected to the detection module, the analysis module and the quorum induction quencher dosing module respectively, and is used to control the operation of the detection module, the analysis module and the quorum induction quencher dosing module respectively.

7. A wastewater denitrification recovery method based on Raman detection and quorum sensing quenching, using the system described in any one of claims 1-6, characterized in that, Includes the following steps: Detect the metabolic impairment state of microorganisms in wastewater due to the influence of microplastics; Based on the metabolic impairment state of microorganisms affected by microplastics, and in combination with the water quality status of the wastewater, the degree to which the wastewater quality is affected by microbial metabolism is determined. Based on the degree to which wastewater quality is affected by microbial metabolism, a quorum sensing quencher is added to the wastewater.

8. The method according to claim 7, characterized in that, The detection of metabolic impairment of microorganisms in wastewater due to microplastics includes: Impurities in the wastewater are filtered out to obtain a microbial mixture; The microbial mixture was centrifuged to obtain a microbial enrichment solution; Add buffer solution to the microbial enrichment solution and adjust the pH value to obtain the microbial detection solution; To detect the metabolically impaired state of microorganisms in microbial test solutions due to the influence of microplastics.

9. The method according to claim 7, characterized in that, Also includes: In response to the determination to add a quorum induction quencher to the wastewater, the water quality health status is detected at preset intervals. If the current water quality health status of the wastewater does not meet the preset recovery standard, the quorum sensing quencher dosing module will be controlled again to add quorum sensing quencher until the water quality health status of the wastewater reaches the preset recovery standard.

10. An electronic device comprising a memory, a processor, and a computer program stored in the memory and running on the processor, characterized in that, When the processor executes the program, it implements the method as described in any one of claims 7 to 9.

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