Method for carrying out bacterium-enzyme composite treatment on grape branch compost

Abstract

The invention discloses a method for carrying out bacterium-enzyme composite treatment on grape branch compost, and belongs to the technical field of agricultural waste resource utilization. According to the method, a bacterial enzyme composite system is prepared by using own saccharomycetes, lactic acid bacteria strains and lignin peroxidase, during composting, grape pruning waste is crushed, the carbon nitrogen ratio is adjusted, a micro-ecological bacterial enzyme complexing agent is inoculated for composting fermentation, the degradation efficiency of cellulose and hemicellulose is enhanced through the synergistic effect of two bacteria and one enzyme, and the composting period is shortened; the high-quality organic fertilizer is obtained. The invention solves the technical problems of slow degradation of grape pruning waste and long composting period, realizes resource utilization of waste, has the advantages of simple operation, low cost and environmental friendliness, and is suitable for large-scale agricultural production and application.

Description

Technical Field

[0001] This invention relates to the field of agricultural waste resource utilization technology, specifically to a method for composting grape branches using a combination of microbial and enzyme treatment. Background Technology

[0002] Grapes, a widely cultivated fruit tree globally, generate a significant amount of winter pruning waste each year. According to our team's statistics, vineyards of different varieties produce approximately 120-170 kg / acre of winter pruning waste annually. These branches are rich in recalcitrant components such as cellulose, hemicellulose, and lignin. Improper disposal or burning not only wastes resources but also causes environmental pollution (e.g., dust and harmful gases produced by burning). Composting is an effective way to utilize grape pruning waste, but traditional composting methods suffer from low degradation efficiency, long composting cycles, and poor decomposition, limiting their large-scale application. In existing technologies, single microbial agents used for waste degradation suffer from limitations such as limited functionality and poor adaptability to complex substrates. In contrast, compound microbial agents, through the synergistic effect of different strains, can complement each other's functions and improve the degradation efficiency of branches. Yeast has a strong ability to decompose carbohydrates and can quickly utilize easily degradable organic matter to generate energy, providing conditions for composting to reach a higher temperature. Lactic acid bacteria can regulate the pH value of the compost microenvironment, inhibit the growth of harmful bacteria, and simultaneously secrete cellulase to assist in the degradation of recalcitrant components. Lignin peroxidase can decompose cellulose and hemicellulose, which are difficult for the above two types of bacteria to decompose. Currently, there are no reports on the efficient composting and degradation of grape pruning waste using a combination of microbial agents and enzymes. Therefore, developing a targeted and synergistic compound microbial agent and its supporting composting method is of significant practical importance. Summary of the Invention

[0003] This invention addresses the problems of difficult lignin degradation, long cycle, and low efficiency in existing grape branch composting technologies. It provides a method for treating grape branch compost using microbial enzymes. By constructing a synergistic system of compound microbial agents, lignin-degrading enzymes, and modified attapulgite soil carrier, the composting cycle is shortened to within 40 days, improving the lignin degradation rate and compost quality, and realizing the efficient resource utilization of grape branches.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: A method for treating grape vine compost with a compound microbial enzyme agent, characterized by comprising three steps: preparation of the compound microbial enzyme agent, pretreatment of grape pruning waste, and compost fermentation, as detailed below: Step 1: Preparation of Compound Microbial Enzyme Agent (1) Strain screening and activation: Select Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiaeThe strain, *Lactobacillus fermentum*, was deposited by the applicant with accession number CGMCC NO.33774, at the China General Microbiological Culture Collection Center (CGMCC) on March 10, 2025, at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Lactobacillus fermentum The strain, deposited by the applicant under accession number CGMCC NO.36375, with the same depositary institution and address as described above, and deposited on October 28, 2025, was used. The yeast and Lactobacillus fermentum were inoculated into YPD medium and cultured at 25-30℃ with shaking at 120-150 rpm for 20-24 h, until the viable count reached 2.0 × 10⁻⁶. 8 -5.0×10 8 CFU / ml; inoculate the above-mentioned *Lactobacillus plantarum* into MRS medium and incubate statically at 30-34℃ for 24 h to activate to a viable count of 5.0 × 10⁻⁶. 8 -8.0×10 8 CFU / ml.

[0005] (2) Preparation of compound microbial agent: Prepare compound fermentation medium, which by mass percentage is molasses 2.0-2.5%, yeast extract 0.6-0.8%, sodium acetate 0.4-0.5%, diammonium hydrogen citrate 0.15-0.2%, tomato juice 5-8%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.015-0.02%, pH adjusted to 6.0, sterilized at 115 ℃ for 20 min; mix the two activated bacterial solutions at a volume ratio of 1:1-1:2, inoculate at 1.5-3% of the fermentation medium volume, and ferment in a deep layer at 28-31 ℃ and 100-120 rpm for 18-24 h, until the pH of the system is 4.0-4.5.

[0006] (3) Preparation of lignin peroxidase: Take the liquid fermentation broth of compound bacterial agent, centrifuge at 8000r / min for 10min, collect the supernatant, add lignin peroxidase, and the enzyme activity is ≥9 U / L.

[0007] (4) Preparation of modified attapulgite carrier: attapulgite was purified and impurities removed, crushed and passed through a 200-mesh sieve, soaked in a 2-3% dilute hydrochloric acid solution for 3 h, stirred at a rate of 50 r / min, and neutralized to pH 6.5-7.0; then dried at 105℃ to constant weight, ultrasonically treated for 20 min to break up the rod crystal bundles, sterilized by high-pressure steam at 121℃ for 30 min, cooled and used for later use. The specific surface area of ​​the modified attapulgite was increased to 180-220 m² / g, which enhanced its adsorption and fixation capacity.

[0008] (5) Assembly of the microbial enzyme complex system: Mix the complex microbial agent with the modified attapulgite soil at a mass ratio of 1:2, stir evenly, add lignin peroxidase, the amount of enzyme added is 1 / 3 of the mass of the complex microbial agent, stir and adsorb at a constant temperature of 25 ℃ for 1-2 h, let stand for 30 min, so that the microorganisms can fully attach to the pores of the attapulgite soil.

[0009] (5) Low-temperature drying: Spread the adsorbed mixture evenly (thickness < 1cm) and place it in a vacuum drying oven or forced-air drying oven at 30-35℃ to dry until the moisture content drops to 8-12% and the bacterial agent concentration is ≥1×10 8 CFU / ml.

[0010] (6) Crushing and sieving: After drying, crush and sieve through an 80-mesh sieve to obtain powdered attapulgite soil fungicide. (7) Stability test: After storage at room temperature for 3 months, the number of viable bacteria should be retested and the survival rate should be ≥ 60%.

[0011] Step 2: Pre-treatment of grape pruning branches (1) Winter pruning: Prune grape fruiting branches or one-year-old branches into branches with a length of 30-200 cm and a cross-sectional diameter of 0.3-2.0 cm; (2) Crushing: Use a crusher to crush the pruned branches. To improve fermentation efficiency and reduce dust, the volume of the crushed branches should be 0.5-3.0 cm³. 3 The shredded branches should have good longitudinal cutting or breaking to increase the surface area of ​​the shredded branches; (3) Adjust the fermentation environment: Use urea to adjust the C:N ratio to 25-30:1 and adjust the moisture content to 10%-20%.

[0012] Step 3: Composting (1) Inoculate the compound microecological agent from step one into the grape pruning waste after pretreatment in step two at an inoculation amount of 1%-5% of the dry weight of the waste, mix evenly, and then compost in windrows or troughs.

[0013] (2) Pile: Pile the crushed branches into a single pile with a volume ≥ 2 m³. 2 The weight of a single pile should be ≥500 kg. Note that the height of the pile should not exceed 2 m, otherwise the bottom will be easily compacted and oxygen-deficient, leading to anaerobic putrefaction.

[0014] (3) Control the temperature of the pile at 55-65℃ for 3-5 days, then let it cool naturally to room temperature. The fermentation cycle is 25-30 days to obtain decomposed organic fertilizer. In the method described in this invention, the particle size of the grape twigs crushed in step 2 is preferably 1.0-2.0 cm. 3Adjust the C:N ratio to 30:1 using urea; alternatively, use a 1:1 mixture of cow manure and chicken manure by mass. In step 3, activate the mixture to a viable bacterial count of 5.0 × 10⁻⁶. 8 -8.0×10 8 CFU / ml, lignin peroxidase activity ≥ 9 U / l, and bacterial agent concentration after drying ≥ 1×10⁻⁶. 8 The viable count after 3 months of storage at room temperature should be ≥ 60% (CFU / ml). The lignin degradation rate should be ≥ 40%, the organic matter content ≥ 45%, and the viable count ≥ 1.0 × 10⁻⁶. 7 CFU / g, pH 5.5-6.5.

[0015] This invention further discloses the application of the method in the resource utilization of waste; the resource utilization of waste refers to its use in orchard soil improvement. Experimental results show that after three years of using grape shredded branches as compost, the soil organic matter content can reach 15.3 g / kg, which is higher than the 8-10 g / kg level of conventional orchards, and basically reaches the 15-20 g / kg level of high-quality and high-yield orchards, demonstrating a significant soil improvement effect.

[0016] Compared with existing technologies, the method for composting grape branches using a combination of microbial and enzyme treatment disclosed in this invention has the following advantages: (1) High degradation efficiency and short cycle: The bacterial enzyme complex system of “self-functional bacteria synergy + lignin degradation enzyme + modified attapulgite soil carrier” is constructed to achieve synergistic effect of bacteria and enzyme. With the help of compound strains and enzyme system, the composting cycle of grape branches is shortened from 60-90 days to about 30 days. The lignin degradation rate is ≥40% and the hemicellulose degradation rate is ≥35%, which is significantly better than traditional composting technology.

[0017] (2) High-quality compost with stable nutrients: precise temperature control in four stages, effective killing of pathogens in the high-temperature stage, nutrient loss rate reduced by more than 20%, pH 5.5-6.5, high humus content, which can improve soil looseness and microbial activity, and meet the soil improvement needs of vineyards.

[0018] (3) Strong stability and controllable cost: Modified attapulgite soil carrier can fix bacteria and enzymes, improve their stress resistance, and adapt to a wide range of environments with pH 3.0-9.0 and temperature 15-35℃; The compound bacterial agent uses its own strains, and the enzyme system can be prepared by fermentation. The raw material cost is low, the process is simple and easy to scale up, solve the problem of grape branch waste treatment, and realize the closed loop of "resource-waste-resource".

[0019] (4) Environmentally friendly and pollution-free: It replaces the traditional incineration treatment method, reduces waste gas emissions, and the composting process has no secondary pollution. The finished organic fertilizer can be returned to the field to reduce the use of chemical fertilizers, promote the development of green agriculture, and has economic, ecological and social benefits. Attached Figure Description

[0020] Figure 1 The impact of piling up and crushing branches on soil organic matter in vineyards. Detailed Implementation

[0021] The present invention will now be described through specific embodiments. Unless otherwise specified, all technical means used in this invention are methods well known to those skilled in the art. Furthermore, the embodiments should be understood as illustrative, not limiting the scope of the invention; the essence and scope of the invention are defined only by the claims. For those skilled in the art, various changes or modifications to the material composition and dosage in these embodiments without departing from the essence and scope of the invention are also within the scope of protection of this invention. The brewing yeast and lactobacillus fermentum used in this invention are proprietary strains; other raw materials and reagents are commercially available. Example

[0022] A method for treating grape vine compost using microbial enzymes, the specific steps of which are as follows: Grape vine pretreatment: Collect grape vines pruned in winter, remove dead leaves and impurities, crush them to a particle size of 1.5-2cm, and adjust the moisture content to 45%.

[0023] Preparation of modified attapulgite carrier: After purification, attapulgite is passed through a 200-mesh sieve, soaked in 2.5% dilute hydrochloric acid for 3 hours, stirred at a rate of 50 r / min, and neutralized to pH 6.8; dried at 105℃ to constant weight, ultrasonicated for 20 minutes, autoclaved at 121℃ for 30 minutes, and cooled for later use.

[0024] Compound microbial enzyme agent: Saccharomyces cerevisiae and Lactobacillus fermentum are mixed at a ratio of 1:2, with an effective viable count of 6.0 × 10⁻⁶. 8 CFU / ml; Take the liquid fermentation broth of the compound bacterial agent, collect the supernatant, add lignin peroxidase, and the enzyme activity is ≥9 U / L.

[0025] System assembly: The compound microbial agent and modified attapulgite soil were mixed at a mass ratio of 1:2, and an enzyme system of 0.5 times the mass of the compound microbial agent was added. The mixture was adsorbed at room temperature for 1 hour to obtain the microbial enzyme compound system.

[0026] Fermentation raw material preparation: Grape crushed branches are mixed with cow manure and chicken manure (1:1) at a mass ratio of 2:1. Add 1.2% of the dry weight of the mixed raw materials to the bacterial enzyme complex system; add urea to adjust the C:N ratio to 28:1, add water to adjust the moisture content to 48%, and the bulk density to 600 kg / m³, and stir evenly.

[0027] Composting Fermentation: **High-Temperature, High-Oxygen Stage (1-5 days):** Promotes rapid microbial growth, raising the compost temperature to above 55℃ within 2 days, maintaining high temperatures to kill pathogens and weed seeds. **High-Temperature, Low-Oxygen Stage (6-15 days):** Maintains the compost temperature at 60-65℃, synergistically decomposing lignin with compound microbial agents. High temperatures are maintained for over 10 days, enhancing degradation efficiency. **Cooling, Low-Oxygen Stage (15-25 days):** The compost temperature naturally drops below 40℃, slowing the metabolic rate of the microbial community. Lignin degradation products are converted into humus, reducing nutrient loss. **Rest at Room Temperature Stage (25-30 days):** Allows the compost to rest at room temperature, completing the composting process. Example

[0028] A method for efficiently degrading grape pruning waste using a compound microecological agent of yeast and lactic acid bacteria, the specific steps of which are as follows: Step 1: Preparation of Compound Microbial Agent 1. Strain activation: The applicant's preserved *Saccharomyces cerevisiae* strain was inoculated onto YPD medium and cultured at 28°C with shaking at 130 rpm for 22 hours. After activation, the viable cell count reached 3.5 × 10⁻⁶. 8 CFU / mL; Lactobacillus fermentum preserved by the applicant was inoculated into MRS medium and incubated statically at 32℃ for 26 h. After activation, the viable count reached 6.8 × 10⁻⁶. 8 CFU / mL.

[0029] 2. Mixed fermentation culture: The compound fermentation medium formula is as follows: molasses 2.2%, yeast extract 0.7%, sodium acetate 0.45%, diammonium citrate 0.18%, tomato juice 6%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.018%, pH adjusted to 6.0, sterilized at 115℃ for 20 min; 2% of the fermentation medium volume is inoculated with a mixed bacterial solution with a volume ratio of 1:1, and deep fermented at 29℃ and 110 rpm for 20 h. Fermentation is stopped when pH=4.2 to obtain the compound bacterial fermentation broth.

[0030] 3. Microbial Agent Formulation: Mix the compound microbial fermentation broth with attapulgite soil at a mass ratio of 1:2, air-dry in a well-ventilated and shady place, controlling the moisture content to 10%, to obtain the compound microecological microbial agent with a total effective viable bacteria count of 1.5 × 10⁻⁶. 9 CFU / g.

[0031] Step 2: Pre-treatment of grape pruning waste Grape twigs and leaves were collected, impurities were removed, and the mixture was crushed to a particle size of 3 cm to obtain a powder. The powder was then soaked in a nutrient solution at 28°C for 24 hours. The nutrient solution consisted of 0.5% urea, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and vitamin B12. 12 0.001%, distilled water balance, pH=6.0; control water content to 15%.

[0032] Step 3: Compound microbial agent degrades grape pruning waste The pretreated crushed material was placed in a fermentation tank, and 10% of the dry weight of the crushed material was added with compound microecological bacteria. After stirring evenly, the material was spread into a 25cm thick layer. The temperature was controlled at 32℃ and the humidity at 70%. Aerobic fermentation was carried out for 4 days, with the material turned over once a day for 18 minutes each time. Then, the material was switched to anaerobic fermentation at 33℃ for 15 days, with ventilation for 6 minutes every 3 days to complete the degradation.

[0033] Step 4: Post-processing of degradation products After degradation, particles larger than 1 cm were removed by sieving to obtain the decomposed product. Testing showed that the lignocellulose degradation rate reached 72%, and the product contained 18.5% humus and 8.2% crude protein, making it suitable as organic fertilizer for vineyards. Example

[0034] Screening for the optimal carbon-nitrogen ratio: Five treatments with carbon-nitrogen ratios of 15, 20, 25, 30, and 35 were set. The total organic carbon and total nitrogen of the samples after fermentation were measured, as well as the organic carbon, total nitrogen, urease, acid phosphatase, and sucrase levels 60 days after fermentation. The carbon-nitrogen ratio and T-value (final T / N / initial T / N) were calculated. Treatments with carbon-nitrogen ratios of 25 and 30 showed better performance and the highest cost-effectiveness. Table 1 shows that the initial carbon-nitrogen ratio of the crushed branches was not significantly different from the set ratios, while the final carbon-nitrogen ratios showed more significant differences. Generally, treatments with lower carbon-nitrogen ratios had lower final values, and treatments with higher ratios had higher final values. When calculating the T-value, only treatments with carbon-nitrogen ratios of 25 and 30 had values ​​below 0.6. A value below 0.6 is generally considered an indicator of complete decomposition and also has some reference value for checking urease activity, acid phosphatase activity, and sucrase activity (Table 1).

[0035]

[0036] Example 4 Screening for optimal moisture content: Five treatments were set up with crushed vine branches containing moisture contents of 5%, 10%, 15%, 20%, and 25%. For each treatment, the total organic carbon and total nitrogen were measured after fermentation, and the organic carbon, total nitrogen, urease, acid phosphatase, and sucrase levels were measured after 60 days of fermentation. The carbon-to-nitrogen ratio and T-value (final T / N value / initial T / N value) were calculated. Table 3 shows that different concentrations of organic composting agents did not significantly affect the initial T / N value, the final T / N value, or the T-value for vine branches with 10%-25% moisture content. After 60 days of composting, the T-values ​​of the grape vine waste were all less than 0.8, significantly higher than the 5% treatment, but all were less than 0.6. This indicates that simply adjusting the vine moisture content cannot promote vine composting. The differences in urease activity, acid phosphatase activity, and sucrase activity were not significant. From the perspective of labor cost control, we believe that controlling the moisture content of branches at low concentrations of 10%, 15%, and 20% has a certain effect on the decomposition of branches (Table 2).

[0037]

[0038] Example 5 (1) Winter pruning: According to the growth habits of grapes, prune the fruiting branches or one-year-old branches into branches with a length of 150cm and a cross-sectional diameter of 1.0cm; (2) Crushing: Use a crusher to crush the pruned branches. To improve fermentation efficiency and reduce dust, the volume of the crushed branches should be 1.0 cm³. 3 The shredded branches should have good longitudinal cutting or breaking to increase the surface area of ​​the shredded branches; (3) Pile: The crushed branches are neatly and regularly piled in the planting trench of the grape vines. The width of the pile of crushed branches is 120cm. In order to achieve a better weed control effect and not hinder the aeration of the soil surface, the thickness of the pile should be 2.0cm. (4) Adjust the fermentation environment: Adjust the carbon-nitrogen ratio (organic carbon: total nitrogen) to 30 according to the weight of the piled branches, use 0.2% organic material composting agent, and adjust the moisture content to 10%. For example, for 1000kg of crushed branches with a moisture content of 5%, the initial carbon-nitrogen ratio is 36, and 2kg of urea, 2kg of organic material composting agent, and 50kg of water need to be added. In specific operation, the calculated urea and organic material composting agent can be dissolved in water, and the solution can be sprayed evenly onto the crushed branches using a sprayer. (5) Sterilization: Disinfect and sterilize the piled grape shredded branches with 1.0% quicklime water; (6) Management after fermentation: The fermentation of grape shredded branches will be completed in 60 days. Depending on the production needs of the vineyard, the shredded branches can be rotary tilled into the ground along with the autumn base fertilizer, or the shredded branches from subsequent years can be piled on top to continue fermentation. Relevant indicators are as follows: Figure 1 As shown.

[0039] Comparative test Using single-strain Saccharomyces cerevisiae and single-strain Lactobacillus fermentum as control groups, and the compound microbial enzyme of this invention as the experimental group, grape pruning waste was treated under the same conditions, with 10 kg of crushed material treated in each group. Table 3 shows that the degradation efficiency and product quality of the compound microbial enzyme of this invention are significantly better than those of single-strain agents, demonstrating clear technical advantages.

[0040]

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any person skilled in the art can make some changes or modifications to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the technical solution of the present invention, and all such modifications and modifications shall fall within the protection scope of the technical solution of the present invention.

Claims

1. A method for treating grape vine compost with a combination of microorganisms and enzymes, characterized in that, The process includes three steps: preparation of compound microbial enzyme agents, pretreatment of grape pruning waste, and composting fermentation, as detailed below: Step 1: Preparation of Compound Microbial Enzyme Agent (1) Strain screening and activation: Select Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae ) strain, Lactobacillus fermentum ( Lactobacillus fermentum The above-mentioned yeast and Lactobacillus fermentum strains were inoculated into YPD medium and cultured at 25-30℃ with shaking at 120-150 rpm for 20-24 h until the viable count reached 2.0 × 10⁻⁶. 8 -5.0×10 8 CFU / ml; inoculate the above-mentioned *Lactobacillus plantarum* into MRS medium and incubate statically at 30-34℃ for 24 h to activate to a viable count of 5.0 × 10⁻⁶. 8 -8.0×10 8 CFU / ml; (2) Preparation of compound microbial agent: Prepare compound fermentation medium, which by mass percentage is molasses 2.0-2.5%, yeast extract 0.6-0.8%, sodium acetate 0.4-0.5%, diammonium hydrogen citrate 0.15-0.2%, tomato juice 5-8%, potassium dihydrogen phosphate 0.2%, manganese sulfate 0.015-0.02%, pH adjusted to 6.0, sterilized at 115 ℃ for 20 min; mix the two activated bacterial solutions at a volume ratio of 1:1-1:2, inoculate at 1.5-3% of the fermentation medium volume, and ferment in a deep layer at 28-31℃ and 100-120 rpm for 18-24 h, until the pH of the system reaches 4.0-4.5; (3) Preparation of lignin peroxidase: Take the liquid fermentation broth of compound bacterial agent, centrifuge at 8000 r / min for 10 min, collect the supernatant, add lignin peroxidase, and the enzyme activity is ≥9 U / L; (4) Preparation of modified attapulgite carrier: attapulgite was purified and impurities removed, crushed and passed through a 200-mesh sieve, soaked in a 2-3% dilute hydrochloric acid solution for 3 h, stirred at a rate of 50 r / min, and neutralized to pH 6.5-7.0; then dried at 105℃ to constant weight, ultrasonically treated for 20 min to break up the rod crystal bundles, sterilized by high-pressure steam at 121℃ for 30 min, cooled and used for later use. The specific surface area of ​​the modified attapulgite was increased to 180-220 m² / g, which enhanced the adsorption and fixation capacity. (5) Assembly of the microbial enzyme complex system: Mix the complex microbial agent with the modified attapulgite soil at a mass ratio of 1:2, stir evenly, add lignin peroxidase, the amount of enzyme added is 1 / 3 of the mass of the complex microbial agent, stir and adsorb at a constant temperature of 25 ℃ for 1-2 h, let stand for 30 min, so that the microorganisms can fully attach to the pores of the attapulgite soil. (5) Low-temperature drying: Spread the adsorbed mixture evenly to a thickness of 0.5-1 cm, and dry it in a vacuum drying oven or forced-air drying oven at 30-35℃ until the moisture content drops to 8-12% and the bacterial agent concentration is ≥1×10⁻⁶. 8 CFU / ml; (6) Crushing and sieving: After drying, crush and pass through an 80-mesh sieve to obtain powdered attapulgite soil fungicide; (7) Stability test: After storage at room temperature for 3 months, the number of viable bacteria should be retested, and the survival rate should be ≥ 60%; Step 2: Pre-treatment of grape pruning branches (1) Winter pruning: Prune grape fruiting branches or one-year-old branches into branches with a length of 30-200 cm and a cross-sectional diameter of 0.3-2.0 cm; (2) Shredding: Use a shredder to shred the pruned branches. The volume of the shredded branches should be 0.5-3.0 cm. 3 ; (3) Adjust the fermentation environment: Use urea to adjust the C:N ratio to 25-30:1 and adjust the moisture content to 10%-20%; Step 3: Composting (1) Inoculate the compound microbial enzyme agent from step one into the grape pruning waste after pretreatment in step two at an inoculation rate of 1%-5% of the dry weight of the waste, mix evenly, and then compost in windrows. (2) Pile: Pile the crushed branches into a single pile with a volume ≥ 2 m³. 2 The weight of a single pile should be ≥500 kg, and the pile height should be 1-2 m. (3) Control the temperature of the pile at 55-65℃ for 3-5 days, then let it cool naturally to room temperature. The fermentation cycle is 25-30 days to obtain decomposed organic fertilizer.

2. The method according to claim 1, characterized in that, In step 3, activate to a viable count of 5.0 × 10⁻⁶. 8 -8.0×10 8 CFU / ml, lignin peroxidase activity ≥ 9 U / l, and bacterial agent concentration after drying ≥ 1×10⁻⁶. 8 The CFU / ml count should be ≥ 60% after 3 months of storage at room temperature.

3. The method according to claim 2, characterized in that, In step 2, the preferred particle size for crushing grape twigs is 1.0-2.0 cm. 3 Adjust the C:N ratio to 30:1 using urea or mix it with sheep manure, cow manure, or chicken manure at a mass ratio of 1-2:

1.

4. The organic fertilizer prepared by the method according to any one of claims 1-3, characterized in that, Lignin degradation rate ≥40%, organic matter content ≥45%, viable bacteria count ≥1.0×10⁷ CFU / g, pH 5.5-6.

5.

5. The application of the method of claim 1 in the resource utilization of waste; wherein the resource utilization of waste refers to its use in orchard soil improvement.

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