Dhx36 gene molecular marker related to bull sperm motility trait and application thereof

By identifying the CG base mutation at position 231 of the bovine DHX36 gene, a specific primer set was designed to detect bovine sperm motility traits, solving the problem of insufficient motility after thawing of frozen bovine semen and achieving efficient genotype selection and genetic improvement.

CN121852566BActive Publication Date: 2026-06-05JILIN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN UNIVERSITY
Filing Date
2026-03-17
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

In existing technologies, the motility of frozen bovine semen after thawing is less than half of that before freezing, resulting in decreased fertilization efficiency. Furthermore, the genetic heterogeneity among individual bulls leads to large fluctuations in motility after thawing, and there is a lack of effective genotype selection methods.

Method used

By identifying the CG base mutation at position 231 of the bovine DHX36 gene, a specific primer set was designed to detect the normal and thawed sperm motility traits of bovine sperm. Genotypic selection was then performed using SNP polymorphism to screen for highly resistant bulls.

Benefits of technology

It significantly improved the post-thaw motility of bovine semen, enhanced batch consistency and quality of frozen semen products, and provided a theoretical basis for marker-assisted selection in cattle.

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Abstract

The application belongs to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the DHX36 gene molecular marker is a C-G base mutation at the 231st site of a bovine DHX36 gene fragment shown in the sequence table SEQ ID NO:1. Based on the DHX36 gene molecular marker, the genotype is determined by sequence determination, and the genotype is associated with the normal and post-thawing sperm vigor trait of the cow. The analysis result shows that the normal and post-thawing sperm vigor of individuals with different genotypes is significantly different. The application uses specific primers to obtain a bovine DHX36 gene fragment related to the normal and post-thawing sperm vigor trait of the cow, and uses a specific SNP site in the fragment as a molecular marker, thereby providing a theoretical basis and specific application for marker-assisted selection of the cow.
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