Animal feed additive and preparation method thereof

Abstract

The invention belongs to the field of additives, and provides an animal feed additive and a preparation method thereof, and the animal feed additive is prepared by mixing a Chinese herbal medicine fermentation liquid and black gadfly pulp according to a mass ratio of 1: (200-500), the Chinese herbal medicine fermentation liquor is prepared by fermenting the following raw materials: 10-15 parts of astragalus membranaceus, 6-10 parts of liquorice, 8-10 parts of salvia miltiorrhiza, 10-12 parts of white paeony root, 10-15 parts of scutellaria baicalensis, 6-12 parts of rheum officinale, 8-12 parts of golden cypress, 10-15 parts of sapium sebiferum, 8-12 parts of tobacco and 4-6 parts of medicated leaven, and the raw materials are crushed and fermented with a compound microbial strain to obtain the Chinese herbal medicine fermentation liquor; the compound microbial strain comprises bacillus subtilis, lactic acid bacteria and bacillus licheniformis, and the viable count ratio of the bacillus subtilis to the lactic acid bacteria to the bacillus licheniformis is (2-3): (1-2): 1; the fermented Chinese herbal medicine is taken as a core, Chinese herbal medicine macromolecules are decomposed into micromolecular active substances (such as fermented astragalus polysaccharide) through microbial conversion of bacillus subtilis and the like, the drug effect is improved by 4-28 times, antibiotic residues are avoided, the problems of drug resistance and drug accumulation are avoided, and the food safety requirement is met.

Description

Technical Field

[0001] This invention belongs to the field of additives, specifically an animal feed additive and its preparation method. Background Technology

[0002] In the animal husbandry industry, feed additives, as core components for maintaining healthy animal growth, directly affect breeding efficiency and product safety. In traditional farming, to prevent interference from microbial diseases and pests on animal growth, feed additives generally rely on antibiotics, such as penicillin, streptomycin, and chlortetracycline, which inhibit or kill pathogens to control diseases. These antibiotic additives have played an important role in improving survival rates and reducing disease losses. However, with long-term and widespread use, their drawbacks have gradually become apparent: the accumulation of antibiotics in animals can be transmitted to humans through the food chain, leading to drug resistance in human pathogens and increasing the difficulty of clinical treatment. At the same time, drug residues in animal products have become a major hidden danger to food safety, triggering multiple public health incidents and posing a continuous threat to consumer health.

[0003] Among existing antibiotic alternatives, single-herb additives are a common choice, utilizing the antibacterial and immune-enhancing properties of natural plant components. However, the active ingredients in traditional Chinese medicine are mostly macromolecules, which have low absorption rates in animal intestines, resulting in insufficient efficacy. Furthermore, their function is concentrated on disease resistance, neglecting the regulation of animal physiological functions and the improvement of immunity. Although ordinary fermentation technology of traditional Chinese medicine can decompose macromolecules of traditional Chinese medicine through microbial action and enhance efficacy, it still lacks systematic TCM formulation design. Most solutions only focus on the fermentation optimization of the traditional Chinese medicine itself, without forming a synergy with high-protein raw materials, making it difficult to balance disease resistance and nutritional supplementation functions, and failing to meet the diverse nutritional needs of animals for overall growth. For example, although some fermented traditional Chinese medicine additives can reduce the incidence of disease in animals, the lack of TCM's concept of nourishing the body's foundation and strengthening the body's functions in the health care and regulation of various animal organs can lead to insufficient absorption and conversion of nutrients such as protein and trace elements, resulting in slow growth and low feed conversion rate. Summary of the Invention

[0004] To address the aforementioned technical problems, this invention provides an animal feed additive and its preparation method, thereby resolving issues such as low absorption rate of active ingredients and limited functionality in existing single-herb additives.

[0005] An animal feed additive and its preparation method, comprising a fermented liquid of traditional Chinese medicine and black horsefly larvae, mixed in a mass ratio of 1:(200-500);

[0006] The fermented liquid of traditional Chinese medicine is made by fermenting the following raw materials: 10-15 parts of Astragalus membranaceus, 6-10 parts of Glycyrrhiza uralensis, 8-10 parts of Salvia miltiorrhiza, 10-12 parts of Paeonia lactiflora, 10-15 parts of Scutellaria baicalensis, 6-12 parts of Rheum palmatum, 8-12 parts of Phellodendron chinense, 10-15 parts of Phellodendron chinense, 8-12 parts of Nicotiana tabacum, and 4-6 parts of Medicated Leaven. After being crushed, it is fermented with compound microbial strains.

[0007] The composite microbial strains include Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis, with a live bacteria ratio of (2-3):(1-2):1;

[0008] The black soldier fly larvae paste is a homogenate prepared by sterilizing and pulverizing black soldier fly larvae, with a protein content of ≥60%.

[0009] Preferably, the preparation of the herbal fermentation liquid includes dry fermentation and wet fermentation stages;

[0010] Dry fermentation: The powdered Chinese herbal medicines are mixed with the compound microbial inoculum at a mass ratio of 10:1 and fermented for 3-5 days at 25-28℃ and 60-70% humidity;

[0011] Wet fermentation: The dry fermentation product is mixed with purified water at a mass ratio of 1:2 and fermented for 2-3 days at 30-32℃, pH 6.5-7.0 and carbon dioxide concentration of 5-8%. The fermentation broth is then obtained by filtration.

[0012] Preferably, the viable bacteria concentration of the composite microbial solution is 1×10⁻⁶. 8 -1×10 9 CFU / mL.

[0013] Preferably, in the preparation of the black soldier fly slurry, the black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and then mixed with an equal amount of purified water to form a homogenate.

[0014] A method for preparing an animal feed additive includes the following steps:

[0015] S1. Pretreatment of Chinese herbal medicines: Weigh out the following herbs according to the proportions: Astragalus membranaceus, Glycyrrhiza uralensis, Salvia miltiorrhiza, Paeonia lactiflora, Scutellaria baicalensis, Rheum palmatum, Phellodendron chinense, Scutellaria baicalensis, Tobacco, and Medicated Leaven. Grind them into powder and pass them through a 60-80 mesh

[0016] S2. Activation of composite microbial strains: Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis are inoculated into a sterile culture medium in a certain proportion and incubated at 37°C for 18-24 hours to achieve a viable bacterial concentration of 1×10⁻⁶. 8 -1×10 9 CFU / mL bacterial suspension;

[0017] S3. Fermentation of Traditional Chinese Medicine:

[0018] ① Dry fermentation: Mix the Chinese herbal medicine powder from step (1) with the bacterial solution from step (2) at a ratio of 10:1, and ferment for 3-5 days at 25-28℃ and 60-70% humidity. Ventilate 1-2 times a day for 30 minutes each time during the fermentation period.

[0019] ② Wet fermentation: Add twice the mass of purified water to the dry fermentation product, adjust the pH to 6.5-7.0, ferment for 2-3 days at 30-32℃ and carbon dioxide concentration of 5-8%, filter and take the supernatant to obtain the Chinese herbal medicine fermentation liquid;

[0020] S4. Preparation of black soldier fly larvae slurry: Black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and mixed with an equal amount of purified water to form a homogenate;

[0021] S5. Compound preparation: Mix the fermented liquid of Chinese herbal medicine with black horsefly slurry at a ratio of (3-5):1, add trace element synergist, stir evenly and then sterilize to obtain the final product.

[0022] Preferably, in step S3 ①, the oxygen concentration of the fermentation system is controlled to be 15-20% during ventilation.

[0023] Preferably, in step S5, the sterilization conditions are pasteurization at 80-90℃ for 15-20 minutes.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. This invention uses fermented Chinese herbal medicines as its core, and transforms them through microorganisms such as Bacillus subtilis to break down the macromolecules of Chinese herbal medicines into small molecule active substances (such as fermented Astragalus polysaccharides), thereby increasing the efficacy by 4-28 times, and without antibiotic residues, avoiding the problems of drug resistance and drug accumulation, and meeting food safety requirements.

[0026] 2. This invention utilizes the high-quality protein (≥60%) and trace elements in black horsefly larvae extract, which, in synergy with probiotics and active enzymes in the fermentation liquid of traditional Chinese medicine, can enhance animal immunity (such as improving immune indicators such as IgG and IgM), supplement nutrition, and improve feed conversion rate. Experiments show that in chicken farming, daily weight gain increases by 10-15%, and mortality rate decreases by 20-30%.

[0027] 3. This invention employs a two-step fermentation process of "dry + wet". Dry fermentation activates microbial metabolism, while wet fermentation promotes the dissolution of active substances. Furthermore, pasteurization preserves the activity of probiotics. The organic form of trace element synergists (such as organic selenium) improves absorption rate and further enhances animal physiological functions. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall process of the present invention. Detailed Implementation

[0029] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0030] As attached Figure 1 As shown:

[0031] Example 1: This invention provides an animal feed additive and its preparation method, comprising a fermented liquid of traditional Chinese medicine and black horsefly larvae, mixed at a mass ratio of 1:(200-500);

[0032] The fermented liquid of traditional Chinese medicine is made by fermenting the following raw materials: 10-15 parts of Astragalus membranaceus, 6-10 parts of Glycyrrhiza uralensis, 8-10 parts of Salvia miltiorrhiza, 10-12 parts of Paeonia lactiflora, 10-15 parts of Scutellaria baicalensis, 6-12 parts of Rheum palmatum, 8-12 parts of Phellodendron chinense, 10-15 parts of Phellodendron chinense, 8-12 parts of Nicotiana tabacum, and 4-6 parts of Medicated Leaven. After being crushed, it is fermented with compound microbial strains.

[0033] The complex microbial strains include Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis, with a live bacteria ratio of (2-3):(1-2):1;

[0034] Black soldier fly larvae paste is a homogenate made from sterilized and pulverized black soldier fly larvae, with a protein content of ≥60%.

[0035] As can be seen from the above, when using it, the staff first pre-processes the Chinese herbal medicines: weigh out 10 parts of Astragalus membranaceus, 6 parts of Glycyrrhiza uralensis, 8 parts of Salvia miltiorrhiza, 10 parts of Paeonia lactiflora, 12 parts of Scutellaria baicalensis, 12 parts of Rheum palmatum, 12 parts of Phellodendron chinense, 10 parts of Scutellaria baicalensis, 8 parts of tobacco, and 4 parts of medicated leaven. After crushing them with a universal grinder and passing them through a 70-mesh sieve, they are mixed to obtain Chinese herbal medicine powder (the particle size of the powder ensures that the microorganisms and raw materials are in full contact during the subsequent fermentation, thereby improving the conversion efficiency).

[0036] Next, the compound microbial strains were activated: Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis were inoculated into a sterile culture medium containing glucose and peptone at a ratio of 2:1:1, and cultured at 37°C in a shaker for 20 hours to achieve a viable bacterial concentration of 5 × 10⁻⁶. 8 CFU / mL bacterial solution (the activated strain has strong metabolic activity and can quickly start fermentation).

[0037] Example 2:

[0038] Reference Figure 1 The preparation of fermented Chinese herbal medicine liquid includes dry fermentation and wet fermentation stages;

[0039] Dry fermentation: The powdered Chinese herbal medicines are mixed with the compound microbial inoculum at a mass ratio of 10:1 and fermented for 3-5 days at 25-28℃ and 60-70% humidity;

[0040] Wet fermentation: The dry fermentation product is mixed with purified water at a mass ratio of 1:2 and fermented for 2-3 days at 30-32℃, pH 6.5-7.0 and carbon dioxide concentration of 5-8%. The fermentation broth is then obtained by filtration.

[0041] As can be seen from the above, during this process, staff need to regularly monitor the pH and temperature of the fermentation system: if the pH is below 6.5, add a small amount of sodium bicarbonate to adjust it; if the temperature exceeds 32°C, turn on the fermenter cooling system (stable environmental parameters ensure stable microbial metabolism and avoid contamination by other microorganisms).

[0042] Example 3:

[0043] Reference Figure 1 In the preparation of black soldier fly larvae slurry, black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and then mixed with an equal amount of purified water to form a homogenate.

[0044] As can be seen from the above, after the preparation of the fermentation liquid of traditional Chinese medicine, black soldier fly slurry is prepared: select fresh black soldier fly larvae, dry them at 65℃ to 8% moisture content, pulverize them through a 90-mesh sieve using an ultra-micro pulverizer, take 1 kg of pulverized material and mix it with 1 kg of purified water, and homogenize it with a colloid mill (homogenization makes the protein easier to be absorbed by the animal intestines).

[0045] Example 4:

[0046] As attached Figure 1 As shown:

[0047] A method for preparing an animal feed additive includes the following steps:

[0048] S1. Pretreatment of Chinese herbal medicines: Weigh out the following herbs according to the proportions: Astragalus membranaceus, Glycyrrhiza uralensis, Salvia miltiorrhiza, Paeonia lactiflora, Scutellaria baicalensis, Rheum palmatum, Phellodendron chinense, Phellodendron chinense, Tobacco, and Medicated Leaven. Grind them into powder and pass them through a 60-80 mesh

[0049] S2. Activation of composite microbial strains: Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis are inoculated into a sterile culture medium in a certain proportion and incubated at 37°C for 18-24 hours to achieve a viable bacterial concentration of 1×10⁻⁶. 8 -1×10 9 CFU / mL bacterial suspension;

[0050] S3. Fermentation of Traditional Chinese Medicine:

[0051] ① Dry fermentation: Mix the Chinese herbal medicine powder from step (1) with the bacterial solution from step (2) at a ratio of 10:1, and ferment for 3-5 days at 25-28℃ and 60-70% humidity. Ventilate 1-2 times a day for 30 minutes each time, and control the oxygen concentration of the fermentation system to 15-20% during ventilation.

[0052] ② Wet fermentation: Add twice the mass of purified water to the dry fermentation product, adjust the pH to 6.5-7.0, ferment for 2-3 days at 30-32℃ and carbon dioxide concentration of 5-8%, filter and take the supernatant to obtain the Chinese herbal medicine fermentation liquid;

[0053] S4. Preparation of black soldier fly larvae slurry: Black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and mixed with an equal amount of purified water to form a homogenate;

[0054] S5. Compound preparation: Mix the fermented liquid of Chinese herbal medicine with black horsefly slurry at a ratio of (3-5):1, add trace element synergist, stir evenly and then sterilize. The sterilization conditions are 80-90℃ pasteurization for 15-20 minutes.

[0055] Working principle: After two-step fermentation, the macromolecules (such as cellulose and polysaccharides) of Chinese herbal medicines are broken down by microorganisms into small molecule active substances (such as astragalus polysaccharides and glycyrrhizic acid), which increases the absorption rate by more than 50%. They can directly act on animal immune cells, enhance antiviral and antibacterial capabilities, and replace the function of antibiotics.

[0056] Black horsefly larvae extract provides high-quality protein and amino acids, which work synergistically with vitamins and active enzymes in the fermentation broth to meet the growth needs of animals; organic trace elements participate in enzyme synthesis, promote metabolism, and improve feed conversion rate.

[0057] Probiotics (such as lactic acid bacteria) produced by compound microbial fermentation enter the animal's intestines, inhibiting the growth of harmful bacteria, regulating the balance of the gut microbiota, reducing the occurrence of diseases such as diarrhea, and promoting the absorption of nutrients, thereby further improving the animal's health and growth performance.

[0058] The embodiments of the present invention are given for the purposes of illustration and description. Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. An animal feed additive, characterized in that: It consists of fermented Chinese herbal medicine liquid and black horsefly larvae paste, mixed in a mass ratio of 1:(200-500); The fermented liquid of traditional Chinese medicine is made by fermenting the following raw materials: 10-15 parts of Astragalus membranaceus, 6-10 parts of Glycyrrhiza uralensis, 8-10 parts of Salvia miltiorrhiza, 10-12 parts of Paeonia lactiflora, 10-15 parts of Scutellaria baicalensis, 6-12 parts of Rheum palmatum, 8-12 parts of Phellodendron chinense, 10-15 parts of Phellodendron chinense, 8-12 parts of Nicotiana tabacum, and 4-6 parts of Medicated Leaven. After being crushed, it is fermented with compound microbial strains. The composite microbial strains include Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis, with a live bacteria ratio of (2-3):(1-2):1; The black soldier fly larvae paste is a homogenate prepared by sterilizing and pulverizing black soldier fly larvae, with a protein content of ≥60%.

2. The animal feed additive as described in claim 1, characterized in that: The preparation of the herbal fermentation liquid includes dry fermentation and wet fermentation stages; Dry fermentation: The powdered Chinese herbal medicines are mixed with the compound microbial inoculum at a mass ratio of 10:1 and fermented for 3-5 days at 25-28℃ and 60-70% humidity; Wet fermentation: The dry fermentation product is mixed with purified water at a mass ratio of 1:2 and fermented for 2-3 days at 30-32℃, pH 6.5-7.0 and carbon dioxide concentration of 5-8%. The fermentation broth is then obtained by filtration.

3. The animal feed additive as described in claim 1, characterized in that: The viable cell concentration of the composite microbial culture solution is 1×10⁻⁶. 8 -1×10 9 CFU / mL.

4. The animal feed additive as described in claim 1, characterized in that: In the preparation of the black soldier fly slurry, the black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and then mixed with an equal amount of purified water to form a homogenate.

5. A method for preparing an animal feed additive, as described in any one of claims 1-4, characterized in that: Includes the following steps: S1. Pretreatment of Chinese herbal medicines: Weigh out the following herbs according to the proportions: Astragalus membranaceus, Glycyrrhiza uralensis, Salvia miltiorrhiza, Paeonia lactiflora, Scutellaria baicalensis, Rheum palmatum, Phellodendron chinense, Scutellaria baicalensis, Tobacco, and Medicated Leaven. Grind them into powder and pass them through a 60-80 mesh S2. Activation of composite microbial strains: Bacillus subtilis, lactic acid bacteria, and Bacillus licheniformis are inoculated into a sterile culture medium in a certain proportion and incubated at 37°C for 18-24 hours to achieve a viable bacterial concentration of 1×10⁻⁶. 8 -1×10 9 CFU / mL bacterial suspension; S3. Fermentation of Traditional Chinese Medicine: ① Dry fermentation: Mix the Chinese herbal medicine powder from step (1) with the bacterial solution from step (2) at a ratio of 10:1, and ferment for 3-5 days at 25-28℃ and 60-70% humidity. Ventilate 1-2 times a day for 30 minutes each time during the fermentation period. ② Wet fermentation: Add twice the mass of purified water to the dry fermentation product, adjust the pH to 6.5-7.0, ferment for 2-3 days at 30-32℃ and carbon dioxide concentration of 5-8%, filter and take the supernatant to obtain the Chinese herbal medicine fermentation liquid; S4. Preparation of black soldier fly larvae slurry: Black soldier fly larvae are dried at 60-70℃ until the moisture content is ≤10%, crushed and passed through an 80-100 mesh sieve, and mixed with an equal amount of purified water to form a homogenate; S5. Compound preparation: Mix the fermented liquid of Chinese herbal medicine with black horsefly larvae slurry at a ratio of 1:(200-500), add trace element synergists, stir evenly and then sterilize to obtain the final product.

6. The preparation method according to claim 5, characterized in that: In step S3 ①, the oxygen concentration of the fermentation system is controlled at 15-20% during ventilation.

7. The preparation method according to claim 5, characterized in that: In step S5, the sterilization conditions are pasteurization at 80-90℃ for 15-20 minutes.

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