Lentinus edodes cultivation method based on liquid strain inoculation

Abstract

The invention discloses a lentinus edodes cultivation method based on liquid strain inoculation. The lentinus edodes cultivation method comprises the following steps: S1, preparing raw materials of a lentinus edodes cultivation compost formula; s2, preparing fungus bags: uniformly mixing the culture materials according to a formula, bagging, sterilizing in a sterilization chamber at normal pressure, taking out and cooling for later use; s3, preparing liquid strain formula raw materials: 10 to 30 parts of potatoes, 2 to 5 parts of agar, 2 to 5 parts of cane sugar, 0.02 to 0.05 part of citric acid and 500 to 700 parts of water; s4, preparing a liquid strain, namely boiling potatoes in boiling water, taking juice, adding cane sugar and citric acid into the juice, sufficiently and uniformly stirring, sterilizing the mixed solution, and culturing in a constant-temperature room, so as to obtain the mushroom liquid strain; s5, inoculating the liquid strain prepared in the step S4 to the fungus bag prepared in the step 2; s6, the fungus bags obtained in the step S5 are placed in a constant-temperature culture room to be cultured, and S7, after the fungus bags obtained in the step S6 are subjected to dark culture, normal fruiting can be achieved.

Description

Technical Field

[0001] This invention relates to the field of mushroom cultivation, specifically to a method for cultivating shiitake mushrooms based on liquid spawn inoculation. Background Technology

[0002] The entire process of using liquid spawn requires aseptic operation, so the requirements for the inoculation environment are extremely high. It must be a Class 10,000 cleanroom, and the cultivation environment must also be kept at a constant temperature. This represents a significant investment in hardware facilities and equipment. After inoculation with shiitake mushroom liquid spawn, the mycelium on the substrate will have fully grown for 25 days, and the safe period will have passed. The subsequent mycelial growth and fruiting stages no longer require an aseptic environment and can be carried out in a normal natural environment.

[0003] However, using existing formulas results in high production costs for shiitake mushrooms and low mycelial biomass, which greatly limits the industrial application of liquid shiitake mushroom spawn. Summary of the Invention

[0004] The purpose of this invention is to provide a method for cultivating shiitake mushrooms based on liquid inoculation, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for cultivating shiitake mushrooms based on liquid inoculation includes the following steps: S1. Prepare the following ingredients for the shiitake mushroom cultivation substrate: 40-89 parts sawdust, 25-90 parts wheat bran, 100-200 parts white sugar, 10-50 parts hemp seed powder, 50-150 parts lespedeza granules, 15-50 parts gypsum, 20-70 parts calcium hydroxide, and 250-400 parts water; or preferably, 53 parts sawdust, 50 parts wheat bran, 170 parts white sugar, 20 parts hemp seed powder, 110 parts lespedeza granules, 35 parts gypsum, 35 parts calcium hydroxide, and 420 parts water.

[0006] S2. Preparation of mushroom bags: Mix the culture medium evenly according to the formula, pack it into bags, sterilize it under normal pressure in a sterilization chamber, remove it and cool it for later use. S3. Prepare the liquid culture formula ingredients: 10-30 parts potato, 2-5 parts agar, 2-5 parts sucrose, 0.02-0.05 parts citric acid and 500-700 parts water; preferably 17 parts potato, 3 parts agar, 3 parts sucrose, 0.02 parts citric acid and 650 parts water.

[0007] S4. Preparation of liquid spawn: Potatoes are boiled in boiling water and the juice is extracted. Sucrose and citric acid are added to the juice and stirred thoroughly. The mixture is then sterilized and cultured in a constant temperature room to obtain liquid spawn of shiitake mushrooms. S5. Inoculate the liquid inoculum obtained in step S4 onto the inoculum bag obtained in step two. S6. Place the mushroom bags obtained in step S5 in a constant temperature incubation room for cultivation. S7. After the mushroom bags obtained in step S6 are cultured in the dark, they will produce mushrooms normally.

[0008] Preferably, the broadleaf tree branch particles and Lespedeza particles in step S1 are treated with ultrasound at a frequency of 70-90 kHz for 15-30 minutes before sterilization, with the ultrasound frequency preferably being 79 kHz and the treatment time preferably being 25 minutes.

[0009] Preferably, the Lespedeza granules in step S1 are sterilized at 100°C for 8-15 hours, preferably 10 hours.

[0010] Preferably, the wood chips in step S1 are a mixture of poplar wood chips, birch wood chips, and Liaodong oak wood chips.

[0011] Preferably, the sterilization chamber temperature in step S2 is 100°C. Preferably, the sterilization time in step S2 is 10-16 hours, preferably 12 hours.

[0012] Preferably, the temperature inside the constant temperature chamber in step S4 is 25±1℃.

[0013] Preferably, the incubation time in the constant temperature room in step S4 is 48 hours.

[0014] Preferably, the culture temperature in step S6 is 22-27℃, more preferably 24℃, and the humidity is 75-80%, more preferably 77%.

[0015] Preferably, the dark culture time in step S7 is 60-64 days, preferably 61 days.

[0016] Compared with the prior art, the beneficial effects of the present invention are: The cultivation method used in this invention uses readily available and common raw materials, which reduces production costs.

[0017] The liquid bacterial strain preparation method of the present invention is simple to operate and easy to promote on a large scale and in an industrial manner.

[0018] This invention adds hemp seed powder to the culture medium, resulting in shiitake mushrooms with a unique flavor and medicinal effects such as laxative, blood pressure reduction, and lowering serum cholesterol levels. Detailed Implementation

[0019] Example 1: A method for cultivating shiitake mushrooms based on liquid inoculation includes the following steps: S1. Prepare the formula for shiitake mushroom cultivation substrate: 53 parts sawdust, 50 parts wheat bran, 170 parts white sugar, 20 parts hemp seed powder, 110 parts lespedeza granules, 35 parts gypsum, 35 parts calcium hydroxide, and 420 parts water. S2. Preparation of culture bags: Mix the culture medium evenly according to the formula, pack it into bags, sterilize it at 100℃ for 12 hours in a sterilization chamber under normal pressure, remove it and cool it for later use.

[0020] S3. Prepare the liquid culture formula ingredients: 17 parts potato, 3 parts agar, 3 parts sucrose, 0.02 parts citric acid and 650 parts water.

[0021] S4. Preparation of liquid spawn: Potatoes are boiled in boiling water and the juice is extracted. Sucrose and citric acid are added to the juice and stirred thoroughly. The mixture is then sterilized and cultured in a constant temperature room to obtain liquid spawn of shiitake mushrooms. S5. Inoculate the liquid inoculum obtained in step S4 onto the inoculum bag obtained in step two. S6. Place the mushroom bags obtained in step S5 in a constant temperature incubation room for incubation at a temperature of 24°C and a humidity of 77%.

[0022] S7. The mushroom bags obtained in step S6 will be cultured in the dark for 61 days, and then the mushrooms will grow normally.

[0023] The Lespedeza granules in step S1 were treated with ultrasound at a frequency of 79 kHz for 25 minutes before sterilization.

[0024] The broadleaf twig particles and Lespedeza particles in step S1 are sterilized at 100°C for 10 hours.

[0025] The wood chips in step S1 are a mixture of poplar wood chips, birch wood chips, and Liaodong oak wood chips.

[0026] Example 2: A method for cultivating shiitake mushrooms based on liquid inoculation includes the following steps: S1. Prepare the formula for shiitake mushroom cultivation substrate: 60 parts sawdust, 83 parts wheat bran, 120 parts white sugar, 20 parts hemp powder, 110 parts lespedeza granules, 35 parts gypsum, 35 parts calcium hydroxide and 420 parts water. S2. Preparation of culture bags: Mix the culture medium evenly according to the formula, pack it into bags, sterilize it at 100℃ for 12 hours in a sterilization chamber under normal pressure, remove it and cool it for later use.

[0027] S3. Prepare the liquid culture formula ingredients: 20 parts potato, 3 parts agar, 2 parts sucrose, 0.02 parts citric acid and 650 parts water.

[0028] S4. Preparation of liquid spawn: Potatoes are boiled in boiling water and the juice is extracted. Sucrose and citric acid are added to the juice and stirred thoroughly. The mixture is then sterilized and cultured in a constant temperature room to obtain liquid spawn of shiitake mushrooms. S5. Inoculate the liquid inoculum obtained in step S4 onto the inoculum bag obtained in step two. S6. Place the mushroom bags obtained in step S5 in a constant temperature incubation room for incubation at a temperature of 24°C and a humidity of 77%.

[0029] S7. The mushroom bags obtained in step S6 will be cultured in the dark for 61 days, and then the mushrooms will grow normally.

[0030] The Lespedeza granules in step S1 were treated with ultrasound at a frequency of 79 kHz for 25 minutes before sterilization.

[0031] The broadleaf twig particles and Lespedeza particles in step S1 are sterilized at 100°C for 10 hours.

[0032] The wood chips in step S1 are a mixture of poplar wood chips, birch wood chips, and Liaodong oak wood chips.

[0033] Example 3: A method for cultivating shiitake mushrooms based on liquid inoculation includes the following steps: S1. Prepare the formula for shiitake mushroom cultivation substrate: 80 parts sawdust, 40 parts wheat bran, 190 parts white sugar, 20 parts hemp seed powder, 140 parts lespedeza granules, 35 parts gypsum, 35 parts calcium hydroxide, and 420 parts water. S2. Preparation of culture bags: Mix the culture medium evenly according to the formula, pack it into bags, sterilize it at 100℃ for 14 hours in a sterilization chamber under normal pressure, remove it and cool it for later use.

[0034] S3. Prepare the liquid culture formula ingredients: 20 parts potato, 3 parts agar, 2 parts sucrose, 0.03 parts citric acid and 650 parts water.

[0035] S4. Preparation of liquid spawn: Potatoes are boiled in boiling water and the juice is extracted. Sucrose and citric acid are added to the juice and stirred thoroughly. The mixture is then sterilized and cultured in a constant temperature room to obtain liquid spawn of shiitake mushrooms. S5. Inoculate the liquid inoculum obtained in step S4 onto the inoculum bag obtained in step two. S6. Place the mushroom bags obtained in step S5 in a constant temperature incubation room for incubation at a temperature of 27°C and a humidity of 80%.

[0036] S7. The mushroom bags obtained in step S6 will be cultured in the dark for 63 days, and then the mushrooms will grow normally.

[0037] The Lespedeza granules in step S1 are treated with ultrasound at a frequency of 80 kHz for 30 minutes before sterilization.

[0038] The broadleaf twig particles and Lespedeza particles in step S1 are sterilized at 100°C for 15 hours.

[0039] The wood chips in step S1 are a mixture of poplar wood chips, birch wood chips, and Liaodong oak wood chips.

Claims

1. A method for cultivating shiitake mushrooms based on liquid spawn inoculation, characterized in that: Includes the following steps: S1. Prepare the formula for shiitake mushroom cultivation substrate: 40-89 parts sawdust, 25-90 parts wheat bran, 100-200 parts white sugar, 10-50 parts hemp seed powder, 50-150 parts lespedeza granules, 15-50 parts gypsum, 20-70 parts calcium hydroxide, and 250-400 parts water. S2. Preparation of mushroom bags: Mix the culture medium evenly according to the formula, pack it into bags, sterilize it under normal pressure in a sterilization chamber, remove it and cool it for later use. S3. Prepare the liquid culture formula ingredients: 10-30 parts potato, 2-5 parts agar, 2-5 parts sucrose, 0.02-0.05 parts citric acid, and 500-700 parts water; S4. Preparation of liquid spawn: Potatoes are boiled in boiling water and the juice is extracted. Sucrose and citric acid are added to the juice and stirred thoroughly. The mixture is then sterilized and cultured in a constant temperature room to obtain liquid spawn of shiitake mushrooms. S5. Inoculate the liquid inoculum obtained in step S4 onto the inoculum bag obtained in step two. S6. Place the mushroom bags obtained in step S5 in a constant temperature incubation room for cultivation. S7. After the mushroom bags obtained in step S6 are cultured in the dark, they will produce mushrooms normally.

2. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: The Lespedeza granules in step S1 are treated with ultrasound at a frequency of 70-90kHz for 15-30 minutes before sterilization.

3. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: The broadleaf twig particles and Lespedeza particles in step S1 are sterilized at 100°C for 8-15 hours.

4. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: The wood chips in step S1 are a mixture of poplar wood chips, birch wood chips, and Liaodong oak wood chips.

5. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: In step S2, the sterilization chamber temperature is 100℃.

6. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: The sterilization time in step S2 is 10-16 hours.

7. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: In step S4, the temperature inside the constant temperature chamber is 25±1℃.

8. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: In step S4, the incubation time in the constant temperature room is 48 hours.

9. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: In step S6, the culture temperature is 22-27℃ and the humidity is 75-80%.

10. The method for cultivating shiitake mushrooms based on liquid inoculation according to claim 1, characterized in that: The dark culture time in step S7 is 60-64 days.