Traditional Chinese medicine enteric-coated preparation for treating pet diarrhea

Abstract

The invention provides a traditional Chinese medicine enteric-coated preparation for treating pet diarrhea, and relates to the technical field of traditional Chinese veterinary medicine pharmacy. The traditional Chinese medicine composition is prepared by subtle compatibility of monarch, minister, assistant and guide according to the traditional Chinese veterinary dialectical treatment principle aiming at spleen-deficiency damp-heat type diarrhea of pet cats and dogs. The traditional Chinese medicine enteric-coated preparation is mainly prepared from radix fici simplicissimae, abrus cantoniensis hance, semen sterculiae nobilis, verbena, polyporus umbellatus, pterocephalus hookeri, coptis chinensis, rehmannia, emblic leafflower fruit, travertine, lotus seeds and liquorice as traditional Chinese medicine raw materials, the whole formula uses cold and heat simultaneously, combines astringency removal and purgation and tonification simultaneously, not only removes the symptom of damp-heat stagnation in the intestinal tract, but also tonifies the root cause of weakness of the spleen and the stomach, and treats both symptoms and root causes. The traditional Chinese medicine enteric-coated preparation has an excellent treatment effect on cat and dog diarrhea caused by various reasons, clinical symptoms such as diarrhea, vomiting and watery stool of sick cats and dogs can be remarkably improved, and a new treatment scheme is provided for pet diarrhea.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine manufacturing technology, specifically to enteric-coated traditional Chinese medicine preparations for treating diarrhea in pets. Background Technology

[0002] With the improvement of economic level and changes in social structure, people's lifestyles and consumption concepts have undergone tremendous changes. The number of pets in China has increased rapidly. The "2025 China Pet Industry White Paper (Consumer Report)" points out that in 2024, the number of dogs and cats in urban areas of China exceeded 120 million. Many pet owners regard their pets as family members, and pet health issues have received widespread attention. The "2023-2024 China Pet Medical White Paper" points out that among the top 10 common diseases sought by dogs and cats, digestive system diseases rank first and second, respectively.

[0003] Dogs and cats can experience digestive problems such as soft stools, diarrhea, and even vomiting under conditions including changes in living environment and diet, pathogen infection, and stress responses such as acute diarrhea. Currently, commonly used clinical treatments and adjunctive therapies include antibiotics such as amoxicillin-clavulanate potassium soluble powder and enrofloxacin injection; anthelmintics such as fenbendazole tablets and praziquantel tablets; astringent and antidiarrheal drugs such as montmorillonite powder, kaolin, and bismuth carbonate tablets; traditional Chinese medicine preparations such as poplar flower oral liquid, Pulsatilla chinensis oral liquid, and Andrographis paniculata injection; and probiotics as pet nutritional supplements to regulate intestinal flora. While effective in preventing and treating intestinal diseases in dogs and cats, in most cases, these medications, when used alone, cannot meet the clinical needs of different conditions, and their palatability is poor, making administration difficult. Commercially available products for preventing and treating digestive problems such as soft stools, diarrhea, and vomiting in dogs and cats often fail to meet the emotional needs of pet owners who want a better treatment experience for their pets.

[0004] This invention researches and develops a traditional Chinese medicine enteric-coated preparation that strengthens the spleen and supports the body's resistance, clears heat and removes dampness, consolidates and nourishes yin, and addresses digestive system problems in dogs and cats such as soft stools, loose stools, watery stools, and even vomiting from multiple dimensions. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] In view of the shortcomings of the prior art, the present invention provides a traditional Chinese medicine enteric-coated preparation for treating pet diarrhea.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine enteric-coated preparation for treating pet diarrhea, wherein the traditional Chinese medicine enteric-coated granules are made from the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of five-finger peach, 10-30 parts of chicken bone grass, 10-30 parts of phoenix eye fruit, 10-30 parts of verbena, 10-30 parts of polyporus umbellatus, 10-30 parts of winged head grass, 10-30 parts of coptis chinensis, 10-30 parts of rehmannia glutinosa, 10-30 parts of amla, 10-30 parts of travertine, 10-30 parts of lotus seed, and 10-30 parts of licorice.

[0010] Furthermore, the enteric-coated Chinese medicine preparation is made from the following Chinese medicinal materials in parts by weight: 15-25 parts of *Ficus hirta*, 15-25 parts of *Abrus precatorius*, 15-25 parts of *Euphorbia hirta*, 15-25 parts of *Verbena officinalis*, 15-25 parts of *Polyporus umbellatus*, 15-25 parts of *Hedyotis diffusa*, 10-20 parts of *Coptis chinensis*, 10-20 parts of *Rehmannia glutinosa*, 10-20 parts of *Phyllanthus emblica*, 10-20 parts of travertine, 10-20 parts of lotus seed, and 10-20 parts of licorice.

[0011] Furthermore, the enteric-coated Chinese medicine preparation is made from the following Chinese medicinal materials in parts by weight: 20 parts of *Ficus hirta*, 20 parts of *Abrus precatorius*, 18 parts of *Echinops latifolius*, 18 parts of *Verbena officinalis*, 20 parts of *Polyporus umbellatus*, 18 parts of *Polygonum aviculare*, 12 parts of *Coptis chinensis*, 14 parts of *Rehmannia glutinosa*, 16 parts of *Phyllanthus emblica*, 16 parts of travertine, 16 parts of lotus seed, and 12 parts of licorice.

[0012] Secondly, the present invention also provides a method for preparing a traditional Chinese medicine enteric-coated preparation for treating pet diarrhea, wherein the traditional Chinese medicine enteric granules are prepared according to the following steps:

[0013] (1) Weigh the following raw materials according to the following weight parts: 10-30 parts of five-finger peach, 10-30 parts of chicken bone grass, 10-30 parts of phoenix eye fruit, 10-30 parts of verbena, 10-30 parts of polyporus umbellatus, 10-30 parts of winged head grass, 10-30 parts of coptis chinensis, 10-30 parts of rehmannia glutinosa, 10-30 parts of amla, 10-30 parts of travertine, 10-30 parts of lotus seed, and 10-30 parts of licorice. Wash, dry, sterilize, pulverize, and pass through an 80-mesh sieve for later use.

[0014] (2) Add 6-8 times the weight of 60-75% ethanol and reflux extract 2-3 times. Combine the extracts, distill under reduced pressure, and concentrate to a paste-like mixture with a relative density of 1.15-1.30.

[0015] (3) Spray dry the above paste mixture to obtain extract powder. Add suitable pharmaceutical excipients and enteric materials to prepare enteric microcapsules, or enteric micro-pellets, or enteric droplets.

[0016] (III) Beneficial Effects

[0017] This invention uses the principles of strengthening the spleen and supporting the body's resistance, clearing heat and removing dampness, and consolidating and nourishing yin as its treatment methods. It targets diarrhea in pet cats and dogs caused by spleen deficiency and damp-heat, following the principles of traditional Chinese veterinary medicine's syndrome differentiation and treatment, and is formulated through a precise combination of principal, assistant, and adjuvant herbs. The formula uses *Ficus hirta* and *Abrus precatorius* as the principal herbs. *Ficus hirta* strengthens the spleen and lungs, resolves dampness and harmonizes the middle jiao, addressing the pathogenesis of spleen deficiency and impaired digestion in cats and dogs, enhancing spleen and stomach function, and supporting the body's resistance. *Abrus precatorius* clears heat and removes dampness, soothes the liver and harmonizes the stomach, addressing the pathogenesis of damp-heat accumulation in the intestines, clearing damp-heat in the liver and gallbladder, and harmonizing the liver and spleen. Together, these two herbs work synergistically to strengthen the spleen and clear heat, treating both the root cause and the symptoms. The formula uses four herbs as assistant herbs: phoenix eye fruit, verbena, polyporus umbellatus, and winged head grass. Phoenix eye fruit helps to eliminate food stagnation and promote digestion, while also astringing and stopping diarrhea, thus assisting the principal herb in restoring the spleen and stomach's digestive function. Verbena invigorates blood circulation, detoxifies, and promotes diuresis and reduces swelling, quickly relieving symptoms such as sticky diarrhea and tenesmus. Polyporus umbellatus promotes diuresis and eliminates dampness, while winged head grass is bitter and cold, detoxifies, cools the blood, and stops dysentery, thus enhancing the heat-clearing effect of the principal herb, chicken bone grass. These four herbs work together as assistant herbs, synergizing with the principal herb from multiple dimensions of promoting digestion, eliminating dampness, and detoxifying, thereby strengthening the effects of clearing damp heat, eliminating food stagnation, and stopping diarrhea. Phyllanthus emblica clears heat and cools the blood, astringes the intestines and stops diarrhea; Nelumbo nucifera strengthens the spleen, stops diarrhea, nourishes the heart and calms the mind, assisting the principal herb in strengthening the spleen and astringing; Coptis chinensis clears heat and dries dampness, drains fire and detoxifies, assisting the principal and assistant herbs in enhancing their heat-clearing power; Rehmannia glutinosa clears heat and cools the blood, nourishes yin and generates fluids; Travertine clears heat, promotes diuresis, astringes the intestines and stops diarrhea. These two herbs act as adjuvant herbs, balancing the cold and heat properties of the entire formula, enhancing the astringent and diarrhea-stopping effect, ensuring that the clearing and draining effects do not harm the body's vital energy. Glycyrrhiza uralensis acts as the guiding herb, harmonizing the various herbs and guiding them to the meridians to relieve pain. The entire formula uses both cold and hot properties, combining clearing and astringing, and employing both attacking and tonifying methods. It not only removes the symptoms of damp-heat accumulation in the intestines but also tonifies the root cause of spleen and stomach weakness, treating both the symptoms and the root cause.

[0018] Animal experiments further demonstrate that the enteric-coated preparation of traditional Chinese medicine in this invention has excellent therapeutic effects on diarrhea in cats and dogs caused by various reasons, specifically in the following aspects:

[0019] 1. The enteric-coated Chinese medicine preparation of the present invention can significantly improve the clinical manifestations of UC model mice, significantly improve the shortening of the colon in UC model mice, help restore colon length, and significantly improve the pathological condition of the colon tissue in UC model mice, reduce inflammatory cells, and has obvious anti-inflammatory effects, and has significant therapeutic effects on UC model mice.

[0020] 2. In a canine diarrhea model induced by senna leaves, the enteric-coated Chinese medicine preparation of this invention significantly improves the clinical symptoms of diarrhea in dogs, rapidly restores their mental and appetite states in a short period of time, reduces the frequency of defecation caused by diarrhea, improves the shape of feces, and can also reduce intestinal inflammatory markers, achieving both symptomatic and radical treatment.

[0021] 3. The enteric-coated Chinese medicine preparation of this invention has a significant therapeutic effect on canine parvovirus enteritis, with a total effective rate of 100%. It can rapidly improve clinical symptoms such as diarrhea, vomiting, and bloody stools in sick dogs, providing a new treatment option for canine parvovirus enteritis. Attached Figure Description

[0022] Figure 1 The results show the colon length measurements for each group of mice.

[0023] Figure 2 These are histopathological images of the colon tissue of mice in each group. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] Example 1

[0026] A traditional Chinese medicine enteric-coated preparation for treating pet diarrhea, wherein the enteric-coated granules are made from the following traditional Chinese medicine raw materials in parts by weight: 10 parts of five-finger peach, 10 parts of chicken bone grass, 10 parts of phoenix eye fruit, 10 parts of verbena, 10 parts of polyporus umbellatus, 10 parts of winged head grass, 10 parts of coptis chinensis, 10 parts of rehmannia glutinosa, 10 parts of amla, 10 parts of travertine, 10 parts of lotus seed, and 10 parts of licorice.

[0027] The traditional Chinese medicine intestinal granule is prepared according to the following steps:

[0028] (1) Weigh the following raw materials according to the following weight parts: 10 parts of five-finger peach, 10 parts of chicken bone grass, 10 parts of phoenix eye fruit, 10 parts of verbena, 10 parts of polyporus umbellatus, 10 parts of winged head grass, 10 parts of coptis chinensis, 10 parts of rehmannia glutinosa, 10 parts of amla, 10 parts of travertine, 10 parts of lotus seed, and 10 parts of licorice. Wash, dry, sterilize, pulverize, and pass through an 80-mesh sieve for later use.

[0029] (2) Add 6-8 times the weight of 60-75% ethanol and reflux extract 2-3 times. Combine the extracts, distill under reduced pressure, and concentrate to a paste-like mixture with a relative density of 1.15-1.30.

[0030] (3) Spray dry the above paste mixture to obtain extract powder. Add suitable pharmaceutical excipients and enteric materials to prepare enteric microcapsules, or enteric micro-pellets, or enteric droplets.

[0031] Example 2

[0032] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 30 parts of five-finger peach, 30 parts of chicken bone grass, 30 parts of phoenix eye fruit, 30 parts of verbena, 30 parts of polyporus umbellatus, 30 parts of winged head grass, 30 parts of coptis chinensis, 30 parts of rehmannia glutinosa, 30 parts of amla, 30 parts of travertine, 30 parts of lotus seed, and 30 parts of licorice.

[0033] Example 3

[0034] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 14 parts of Five-finger Peach, 12 parts of Chicken Bone Grass, 14 parts of Phoenix Eye Fruit, 12 parts of Verbena, 14 parts of Polyporus umbellatus, 12 parts of Winged Head Grass, 12 parts of Coptis chinensis, 12 parts of Rehmannia glutinosa, 12 parts of Phyllanthus emblica, 12 parts of Calcite, 14 parts of Lotus Seed, and 12 parts of Licorice.

[0035] Example 4

[0036] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 28 parts of Five-Finger Peach, 26 parts of Chicken Bone Grass, 26 parts of Phoenix Eye Fruit, 28 parts of Verbena officinalis, 24 parts of Polyporus umbellatus, 26 parts of Winged Head Grass, 22 parts of Coptis chinensis, 26 parts of Rehmannia glutinosa, 24 parts of Phyllanthus emblica, 22 parts of Calcite, 24 parts of Lotus Seed, and 14 parts of Licorice.

[0037] Example 5

[0038] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 15 parts of five-finger peach, 15 parts of chicken bone grass, 15 parts of phoenix eye fruit, 15 parts of verbena, 15 parts of polyporus umbellatus, 15 parts of winged head grass, 10 parts of coptis chinensis, 10 parts of rehmannia glutinosa, 10 parts of amla, 10 parts of travertine, 10 parts of lotus seed, and 10 parts of licorice.

[0039] Example 6

[0040] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 25 parts of Five-Finger Peach, 25 parts of Chicken Bone Grass, 25 parts of Phoenix Eye Fruit, 25 parts of Verbena officinalis, 25 parts of Polyporus umbellatus, 25 parts of Winged Head Grass, 20 parts of Coptis chinensis, 20 parts of Rehmannia glutinosa, 20 parts of Phyllanthus emblica, 20 parts of Travertine, 20 parts of Lotus Seed, and 20 parts of Licorice.

[0041] Example 7

[0042] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 18 parts of Five-Finger Peach, 16 parts of Chicken Bone Grass, 16 parts of Phoenix Eye Fruit, 16 parts of Verbena, 16 parts of Polyporus umbellatus, 16 parts of Winged Head Grass, 10 parts of Coptis chinensis, 10 parts of Rehmannia glutinosa, 14 parts of Phyllanthus emblica, 12 parts of Calcite, 12 parts of Lotus Seed, and 10 parts of Licorice.

[0043] Example 8

[0044] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 22 parts of Five-Finger Peach, 22 parts of Chicken Bone Grass, 20 parts of Phoenix Eye Fruit, 20 parts of Verbena officinalis, 22 parts of Polyporus umbellatus, 20 parts of Winged Head Grass, 14 parts of Coptis chinensis, 14 parts of Rehmannia glutinosa, 14 parts of Phyllanthus emblica, 14 parts of Calcite, 14 parts of Lotus Seed, and 12 parts of Licorice.

[0045] Example 9

[0046] The difference between this embodiment and Embodiment 1 is that the enteric-coated Chinese medicine preparation is made from the following Chinese medicine raw materials in parts by weight: 20 parts of *Ficus hirta*, 20 parts of *Abrus precatorius*, 18 parts of *Echinops latifolius*, 18 parts of *Verbena officinalis*, 20 parts of *Polyporus umbellatus*, 18 parts of *Polygonum aviculare*, 12 parts of *Coptis chinensis*, 14 parts of *Rehmannia glutinosa*, 16 parts of *Phyllanthus emblica*, 16 parts of *Trametes versicolor*, 16 parts of *Nelumbo nucifera*, and 12 parts of *Glycyrrhiza uralensis*.

[0047] Experimental Example 1

[0048] Effects of the enteric-coated traditional Chinese medicine preparation of this invention on a mouse model of DSS-induced ulcerative colitis

[0049] 1. Materials and Methods

[0050] 1.1 Laboratory Animals

[0051] Fifty 6-week-old SPF-grade male C57BL / 6J mice were selected and housed in an SPF-grade environment, following a diurnal cycle, with suitable temperature and humidity maintained. The indoor temperature was 22–26℃ and the relative humidity was 50%–60%.

[0052] 1.2 Preparation of the Traditional Chinese Medicine Preparation of the Invention

[0053] Weigh out the following ingredients according to the proportions: 20 parts of *Ficus hirta*, 20 parts of *Abrus precatorius*, 18 parts of *Echinops latifolius*, 18 parts of *Verbena officinalis*, 20 parts of *Polyporus umbellatus*, 18 parts of *Hedyotis diffusa*, 12 parts of *Coptis chinensis*, 14 parts of *Rehmannia glutinosa*, 16 parts of *Phyllanthus emblica*, 16 parts of *Trametes verticillata*, 16 parts of *Nelumbo nucifera*, and 12 parts of *Glycyrrhiza uralensis*. After washing, soak them in 4 times the amount of purified water for 30 minutes, then decoct over high heat for 30 minutes. Repeat the decoction twice, combine the decoctions, and concentrate them to a medicinal content of 1 g / mL. Refrigerate for later use.

[0054] 1.3 Animal grouping, model establishment, and drug administration

[0055] Before the experiment, mice were acclimatized for one week and then randomly divided into five groups using a random number table: a blank control group, a model group, a positive control group, a low-dose traditional Chinese medicine (TCM) preparation group, and a high-dose TCM preparation group, with 10 mice in each group. The blank control group was fed normally and had free access to sterilized mineral water. The remaining four groups of mice had free access to a solution containing 3% DSS for 7 days to establish a mouse model of ulcerative colitis (UC). During the modeling period, the positive control group was administered berberine hydrochloride tablets (200 mg / kg / d), while the low-dose and high-dose TCM preparation groups were administered the TCM preparation (100 mg / kg / d and 200 mg / kg / d, respectively) by gavage. The blank control group and the model group were administered an equal volume of ultrapure water by gavage. All groups were administered the medication once daily for 7 consecutive days.

[0056] 1.4 Observation Indicators

[0057] 1.4.1 Mouse body weight, fecal condition, and bloody stool

[0058] Mouse weight, fecal condition, and bloody stool were recorded, and the Disease Activity Index (DAI) was calculated based on references. Specific scoring criteria are shown in Table 1.

[0059] Table 1 DAI Scoring Criteria

[0060]

[0061] DAI score = (weight loss score + stool form score + rectal bleeding score) / 3

[0062] 1.4.2 Colon length measurement

[0063] After treatment, the mice were euthanized by cervical dislocation. The intestinal tissue from the end of the cecum to the anus was separated, and the length from the anus to the rectum to the junction of the colon and cecum was measured.

[0064] 1.4.3 Histopathological observation of colon

[0065] Inflammated colon tissue from mice was rinsed twice with pre-cooled 0.9% sodium chloride injection. A 1 cm section of rectal tissue near the anus was soaked in paraformaldehyde for 24 hours, dehydrated, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (HE). The tissue was then observed and analyzed under a microscope.

[0066] 1.5 Statistical Analysis

[0067] Statistical analysis was performed using Prism 8.0, and one-way ANOVA was used for inter-group comparisons. Results are expressed as mean ± standard deviation. The results were analyzed using a t-test. P < 0.05 was considered statistically significant.

[0068] 2 Results

[0069] 2.1 Effects of the herbal preparation of this invention on the disease activity index of UC model mice

[0070] The results are shown in Table 2. Compared with the control group, the model group mice showed a significant decrease in body weight, loose stools, obvious bloody stools, and a significant increase in DAI scores, indicating that the UC model mouse was successfully established. On day 3 of the experiment, compared with the model group, the symptoms of mice in all treatment groups were significantly alleviated, and the DAI scores of the positive control group and the high-dose group were significantly reduced (P<0.01), while the DAI score of the low-dose group was significantly reduced (P<0.05). On day 7 of the experiment, compared with the model group, the DAI scores of all treatment groups were significantly reduced (P<0.01), with the DAI score of the high-dose group being significantly lower than that of the positive control group. These results indicate that the enteric-coated preparation of traditional Chinese medicine in this invention can significantly improve the clinical manifestations of UC model mice, demonstrating a significant therapeutic effect.

[0071] Table 2. DAI scores of mice in each group

[0072]

[0073] Note: Compared with the blank group, # P<0.05, ## P<0.01; compared with the model group, *P<0.05, **P<0.01.

[0074] 2.2 Effects of the herbal preparation of this invention on colon length in UC model mice

[0075] Colonic shortening can occur due to either insufficient blood supply to the colon leading to local ischemia and necrosis, or extensive inflammatory cell infiltration damaging the colonic tissue. The colonic length measurements for each group of mice are as follows: Figure 1 As shown in the figure, the colon length of mice in the model group was significantly shortened compared to the control group (P<0.01). Compared to the model group, the colon length of mice in each treatment group was significantly increased (P<0.01), with the colon length of mice in the high-dose and low-dose groups being significantly higher than that of the positive control group. These results indicate that the enteric-coated preparation of traditional Chinese medicine in this invention can significantly improve the shortened colon in UC model mice and help restore colon length.

[0076] 2.3 Effects of the traditional Chinese medicine preparation of this invention on the histopathology of colon tissue in UC model mice

[0077] The results are as follows Figure 2As shown, the colonic tissue of the control group mice consists of the mucosa, submucosa, muscularis propria, and serosa. The mucosa protrudes into the lumen to form folds, and the surface of the folds is covered with a single layer of columnar epithelium, interspersed with numerous goblet cells. The lamina propria is dense. Compared with the control group, significant colitis features were observed in the colonic tissue of the model group UC mice, including intestinal wall mucosal erosion and ulceration, loss of crypts, inflammatory cell infiltration, and depletion of goblet cells. The positive control group UC mice showed poor intestinal wall mucosal integrity, and no improvement in the number of goblet cells. The colonic mucosal morphology of the low-dose and high-dose groups of UC mice was similar to that of the control group, and the number of goblet cells was basically the same as that of the control group, indicating that the pathological changes were controlled and improved. These results indicate that the enteric-coated preparation of traditional Chinese medicine in this invention can significantly improve the pathological condition of the colonic tissue of UC model mice and reduce inflammatory cells.

[0078] Experimental Example 2

[0079] 1. Animal grouping and treatment

[0080] Twenty healthy Schnauzer dogs, weighing 7±0.5 kg and fully vaccinated, were selected and fed a basic dog diet with free access to water for 3 days. They were then randomly divided into 4 groups of 5 dogs each: a control group, a model group, a positive control group, and an experimental group. The control group was fed regular dog food. For the first 3 days of the experiment, the other groups were fed senna leaves (2 g / kg) once a day for 3 consecutive days in addition to the treatment given to the control group, to establish a canine diarrhea model.

[0081] After successful model construction, the blank control group and the model group were fed normally without any treatment; the positive control group was fed enrofloxacin tablets 5 mg / kg in addition to the blank control group treatment; the experimental group was fed enteric-coated microgranules prepared in Example 9 0.2 g / kg in addition to the blank control group treatment; each group was administered the medication once daily for 3 consecutive days. Changes in the clinical condition of the dogs in each group were observed.

[0082] 2. Observation and detection indicators

[0083] 2.1 Stool characteristics score

[0084] Diarrhea was classified and scored according to the Bristol fecal grading system. See Table 3 for details.

[0085] Table 3. Scoring Criteria for Diarrhea Severity

[0086]

[0087] 2.2 Detection of C-Reactive Protein (CRP) Content

[0088] Before and after drug administration, whole blood was collected from each group of dogs, and the CRP content in the serum of each group of dogs was detected using an immunofluorescence analyzer.

[0089] 3 Results

[0090] 3.1 Clinical Manifestations and Symptoms

[0091] Before the experiment, all dogs were in good spirits, had normal appetites, and their visible mucous membranes were pale pink and moist. After the canine diarrhea model was established, the control group had normal defecation, while the modeled groups showed lethargy, disheveled fur, significantly increased defecation frequency, loose stools (sometimes watery), and fecal contamination of the anus and tail base. This indicated that the canine diarrhea model was successfully established. After treatment with the appropriate medications, the control group showed no significant changes, with normal spirits, appetite, and defecation; the model group experienced persistent diarrhea, lethargy, reduced food intake, and loose stools; the positive control group and the experimental group showed significant improvement in diarrhea. The positive control group showed good spirits, moderate appetite, and occasional mild loose stools; the experimental group showed a return to normal spirits, appetite, and defecation.

[0092] 3.2 Stool Shape Score

[0093] The results are shown in Table 4. Before administration, compared with the blank group, the fecal shape scores of dogs in the model group, positive control group, and experimental group were significantly increased (P<0.05). All dogs in each group showed diarrhea symptoms, with severe cases exhibiting watery stools. After administration, compared with the model group, the fecal shape scores of dogs in the positive control group and experimental group were significantly decreased (P<0.05); among them, the fecal shape score of dogs in the experimental group was significantly lower than that in the positive control group (P<0.05), but there was no significant difference compared with the blank group (P>0.05).

[0094] Table 4 Stool Shape Scoring

[0095]

[0096] Note: In the same column of data, the same lowercase abbreviation indicates no significant difference (P>0.05); different lowercase abbreviations indicate significant differences (P<0.05).

[0097] 3.3 CRP content

[0098] The results are shown in Table 5. Before administration, compared with the blank group, the CRP levels in the model group, positive control group, and experimental group were significantly increased (P<0.05), indicating that senna leaf-induced diarrhea led to acute enteritis symptoms in dogs. After administration, compared with the model group, the CRP levels in the positive control group and experimental group were significantly decreased (P<0.05); among them, the CRP levels in the experimental group were comparable to those in the positive control group, with no significant difference (P>0.05).

[0099] Table 5. Serum CRP levels (mg / L) in each group of dogs.

[0100]

[0101] Note: In the same column of data, the same lowercase abbreviation indicates no significant difference (P>0.05); different lowercase abbreviations indicate significant differences (P<0.05).

[0102] 4. Conclusion

[0103] The enteric-coated Chinese medicine preparation of this invention can significantly improve the clinical symptoms of diarrhea in dogs, quickly restore their mental and appetite state, reduce the frequency of defecation caused by diarrhea, improve the shape of feces, and at the same time reduce intestinal inflammation indicators, thus achieving both symptomatic and radical treatment.

[0104] Application examples

[0105] Case details

[0106] Forty dogs (half male and half female) under the age of 12 months were selected from multiple veterinary hospitals and diagnosed with canine parvovirus enteritis between January 2024 and March 2025.

[0107] Diagnostic criteria: Clinical symptoms include vomiting, diarrhea, loose stools, rapid rise in body temperature over a short period, lethargy, loss of appetite, and bloody stools. The test result is positive using a CPV colloidal gold test card.

[0108] Forty sick dogs were randomly divided into two groups: a control group and an observation group, with 20 dogs in each group.

[0109] 2 Treatment methods

[0110] Basic supportive care:

[0111] Treatment should be based on the pathological process and clinical symptoms. (1) Maintain acid-base and electrolyte balance: Intravenous drip of compound sodium chloride, 80 mL / (kg·d), for 5 consecutive days; (2) Maintain energy, strengthen the heart and fight inflammation: Intravenous drip of 0.5 g / kg of 5% glucose saline to supplement energy, once a day, for 5 consecutive days; Add ATP 20 mg / kg, vitamin C 50 mg / kg, coenzyme A 50 U / kg and inosine 2 mg / kg to glucose solution as appropriate according to symptoms to strengthen the heart and fight inflammation, once a day, for 2-3 consecutive days. (3) Stop bleeding and stop vomiting: 5 days is one course of treatment. For the first 3 days, inject 2 mL / time of ethamsylate into the muscle, and for the last 2 days, inject 20 mg / time of vitamin K3 into the muscle to stop bleeding, both twice a day; inject 0.5 mL / kg of metoclopramide into the muscle to stop vomiting, twice a day, for 3 consecutive days. (4) Prevent secondary infection: Intravenous drip of ceftriaxone sodium 5mg / kg once a day for 5 days.

[0112] Control group: In addition to supportive therapy and symptomatic control, refined canine pentavalent serum 2 mL / kg was subcutaneously injected once a day for 5 days.

[0113] Treatment group: In addition to supportive therapy and symptomatic control, the patients were fed the enteric-coated microcapsules of the traditional Chinese medicine prepared in Example 9 of this invention, 0.2 g / kg, twice a day, for 5 consecutive days.

[0114] 3 Cure Standards

[0115] Significant effect: Within 3 days of treatment, the dog's mental state, body temperature, appetite, defecation and heart rate all returned to normal, and there was no vomiting.

[0116] Effective: Within 5 days of treatment, the dog's mental state, body temperature, appetite, defecation, and heart rate returned to normal, and there was no vomiting.

[0117] Ineffective: No improvement in clinical symptoms or death of the dog.

[0118] 4. Treatment effect

[0119] The results are shown in Table 6. The total effective rate in the treatment group was 100%, while the total effective rate in the control group was 85%. These results indicate that the enteric-coated Chinese medicine preparation of this invention has a significant therapeutic effect on canine parvovirus enteritis and can rapidly improve the clinical symptoms of sick dogs.

[0120] Table 6 Clinical efficacy of the two groups

[0121]

[0122] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine enteric-coated preparation for treating diarrhea in pets, characterized in that, The enteric-coated granules of the traditional Chinese medicine are made from the following parts by weight of traditional Chinese medicine raw materials: 10-30 parts of five-finger peach, 10-30 parts of chicken bone grass, 10-30 parts of phoenix eye fruit, 10-30 parts of verbena, 10-30 parts of polyporus umbellatus, 10-30 parts of winged head grass, 10-30 parts of coptis chinensis, 10-30 parts of rehmannia glutinosa, 10-30 parts of amla, 10-30 parts of travertine, 10-30 parts of lotus seed, and 10-30 parts of licorice.

2. The enteric-coated traditional Chinese medicine preparation for treating pet diarrhea as described in claim 1, characterized in that, The enteric-coated Chinese medicine preparation is made from the following Chinese medicinal materials in parts by weight: 15-25 parts of *Ficus hirta*, 15-25 parts of *Abrus precatorius*, 15-25 parts of *Euphorbia hirta*, 15-25 parts of *Verbena officinalis*, 15-25 parts of *Polyporus umbellatus*, 15-25 parts of *Polygonum multiflorum*, 10-20 parts of *Coptis chinensis*, 10-20 parts of *Rehmannia glutinosa*, 10-20 parts of *Euphorbia humifusa*, 10-20 parts of *Trametes versicolor*, 10-20 parts of *Lysimachia christinae*, 10-20 parts of *Nelumbo nucifera*, and 10-20 parts of *Glycyrrhiza uralensis*.

3. The enteric-coated traditional Chinese medicine preparation for treating pet diarrhea as described in claim 1, characterized in that, The enteric-coated Chinese medicine preparation is made from the following Chinese medicinal materials in parts by weight: 20 parts of five-finger peach, 20 parts of chicken bone grass, 18 parts of phoenix eye fruit, 18 parts of verbena, 20 parts of polyporus umbellatus, 18 parts of winged head grass, 12 parts of coptis chinensis, 14 parts of rehmannia glutinosa, 16 parts of amla, 16 parts of travertine, 16 parts of lotus seed, and 12 parts of licorice.

4. A method for preparing an enteric-coated traditional Chinese medicine preparation for treating pet diarrhea, characterized in that, The traditional Chinese medicine intestinal granule is prepared according to the following steps: (1) Weigh the following raw materials according to the following weight parts: 10-30 parts of five-finger peach, 10-30 parts of chicken bone grass, 10-30 parts of phoenix eye fruit, 10-30 parts of verbena, 10-30 parts of polyporus umbellatus, 10-30 parts of winged head grass, 10-30 parts of coptis chinensis, 10-30 parts of rehmannia glutinosa, 10-30 parts of amla, 10-30 parts of travertine, 10-30 parts of lotus seed, and 10-30 parts of licorice. Wash, dry, sterilize, pulverize, and pass through an 80-mesh sieve for later use. (2) Add 6-8 times the weight of 60-75% ethanol and reflux for extraction, 2-3 times. Combine the extracts, distill under reduced pressure, and concentrate to a paste-like mixture with a relative density of 1.15-1.

30. (3) Spray dry the above paste mixture to obtain extract powder. Add suitable pharmaceutical excipients and enteric materials to prepare enteric microcapsules, or enteric micro-pellets, or enteric droplets.

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