Closed butt joint and sterile transfer device for cell samples

Abstract

The invention relates to the technical field of cell sample transfer culture, in particular to a cell sample closed butt joint and sterile transfer device which comprises an intelligent closed transfer box, an inner and outer transfer unit, a suspension supporting assembly, a chassis adsorption unit, a cover body storage mechanism and a grabbing transfer unit. The grabbing and transferring unit can transfer the culture dishes in the inner and outer transferring unit to the upper side of the base plate adsorption unit after the base plate adsorption unit adsorbs the bottoms of the culture dishes. Petri dishes transferred into the intelligent closed transfer box by the internal and external transfer unit can be grabbed to the upper side of the chassis adsorption unit through the grabbing and transferring unit, the chassis adsorption unit adsorbs the bottoms of the petri dishes, and the grabbing and transferring unit separates top covers of the petri dishes from the bottoms of the petri dishes; meanwhile, the suspension supporting assembly supports the culture dish in a suspension mode through the chassis adsorption unit, so that the culture dish can be fully placed in the internal environment of the intelligent closed transfer box.

Description

Technical Field

[0001] This invention relates to the technical field of cell sample transport and culture, and in particular to a closed docking and aseptic transport device for cell samples. Background Technology

[0002] The biological big data sharing platform is a digital infrastructure that integrates, stores, manages, and shares massive amounts of data in the life sciences field. It aims to break down data silos, promote cross-institutional and interdisciplinary collaboration, and accelerate life science research and translational applications.

[0003] Cell culture is a core method for obtaining homogeneous and reproducible cell samples in the laboratory (as opposed to primary cells directly obtained from the body), and is particularly suitable for long-term tracking, batch experiments, and model construction. A large amount of omics data (genomics, transcriptomics, proteomics, etc.) in the platform relies on a stable supply of cultured cells. To reduce cell contamination during cell sample transport, sealed and sterile equipment is used, and the cell samples continue to be cultured during transport, providing the necessary environment for the culture device while it is in motion.

[0004] Chinese patent CN217127443U, related to cell culture, discloses a biological culture device. Its technical solution includes: a protective box, a base, and a protective plate. The protective box is fixedly installed on the top of the base. The interior of the protective box contains a storage chamber, a transfer chamber, and an observation chamber. An ultraviolet disinfection lamp is fixedly installed on the inner wall of the transfer chamber. A microbial isothermal anaerobic cell chamber is placed inside the observation chamber. A protective plate is fixedly installed at the front end of the protective box. This invention, through the cooperation of the protective box, protective plate, observation chamber, and rubber gloves, allows for convenient removal of culture samples to the observation chamber for observation, protecting the culture samples from contamination. The cooperation of the transfer chamber, ultraviolet disinfection lamp, and sealed door facilitates the transfer of culture samples from the transfer chamber to the observation chamber, preventing contamination of the culture samples with bacteria or other harmful substances that could affect the culture results.

[0005] The aforementioned related technologies and existing technologies regarding cell sample transport have the following drawbacks: In cell culture, cells are generally placed in a culture dish, with the culture portion and the top cover portion having an interference fit, creating a certain tension between the top cover and the culture dish to ensure the internal sealing of the culture dish. However, to ensure normal cell culture during transport, if the culture dish is placed in the transport device with the top cover sealed, the oxygen concentration inside the sealed culture dish cannot be replenished in time during long-term transport. Conversely, if the dish is placed inside the device with the top cover open, it will cause the cells to come into contact with the external space, leading to infection. Summary of the Invention

[0006] To address the problems mentioned in the background art, the present invention provides a closed docking and sterile transport device for cell samples.

[0007] This invention provides a closed-loop docking and aseptic transport device for cell samples, employing the following technical solution: It includes an intelligent closed transport box, internal and external transport units, a suspension support assembly, a chassis adsorption unit, a cover storage mechanism, and a gripping and transfer unit. There are two internal and external transfer units, both of which are installed inside the intelligent closed transfer box. The internal and external transfer units can transfer the cell culture dish from the outside to the inside. The suspension support assembly is coaxially installed inside the intelligent enclosed transfer box, and the intelligent enclosed transfer box can drive the suspension support assembly to rotate along the axis. The chassis adsorption unit is connected to the suspension support assembly, with the adsorption end of the chassis adsorption unit facing upwards, and the chassis adsorption unit adsorbs the culture dish placed on the upper side. The cover storage mechanism is installed at the axis of the intelligent closed transfer box, and the suspension support component is installed inside the cover storage mechanism. The suspension support component rotates relative to the cover storage mechanism, and the upper opening of the cover storage mechanism extends out of the upper side of the suspension support component. The gripping and transfer unit is installed inside the intelligent closed transfer box. The gripping and transfer unit can transfer the culture dish in the inner and outer transfer unit to the upper side of the chassis adsorption unit. After the chassis adsorption unit adsorbs the bottom of the culture dish, the gripping and transfer unit can separate the top cover of the culture dish from the bottom of the culture dish. The gripping and transfer unit transfers the removed cover to the cover storage mechanism.

[0008] Optionally, the internal and external transfer unit includes: The transfer side box is installed through the circumference of the intelligent closed transfer box, and the upper surface of the outer part of the transfer side box has an insertion opening. A one-way gas extractor is installed on the upper surface of the outer part of the transfer side box located in the intelligent closed transfer box; It also includes a transfer tray, which is fitted inside the transfer side box. The intelligent sealed transfer box can drive the transfer tray to rotate. The circumferential side of the transfer tray has an upward-opening transfer groove. A one-way gas extractor can extract the air inside the connected transfer groove.

[0009] Optionally, the suspension support assembly includes a suspension plate, which is suspended and rotatably installed inside the intelligent enclosed transfer box. The intelligent enclosed transfer box drives the suspension plate to rotate, and the suspension plate rotates and is sleeved on the outside of the cover storage mechanism. Multiple placement slots are opened on the upper surface of the suspension plate, and the adsorption ends of the chassis adsorption unit are distributed inside the multiple placement slots.

[0010] Optionally, the chassis adsorption unit includes a support plate, and multiple support plates are provided. The multiple support plates are installed one-to-one in the multiple placement slots, and a bottom suction cup is fixed on the upper surface of the support plate.

[0011] Optionally, the cover storage mechanism includes a central storage cylinder, the lower end of which is installed at the center of the bottom of the intelligent enclosed transfer box, the upper end of which is open, the central storage cylinder is located on the side of the suspension plate axis, and a tray is provided inside the central storage cylinder. The intelligent enclosed transfer box drives the tray to move up and down inside the central storage cylinder.

[0012] Optionally, the grasping and transferring unit includes: A horizontal frame is installed horizontally inside the intelligent enclosed transfer box. The horizontal frame is set on the upper side of the suspension plate. The intelligent enclosed transfer box can drive the horizontal frame to move up and down, and the intelligent enclosed transfer box can drive the horizontal frame to rotate around the axis of the suspension plate. A hollow meshing block is slidably inserted into the inside of a crossbeam. A threaded shaft capable of power rotation is installed inside the crossbeam, and the hollow meshing block is threadedly sleeved on the outer surface of the threaded shaft. A gripping disc is located below a hollow meshing block. The gripping disc and the hollow meshing block are connected by a telescopic connecting pipe. Both ends of the telescopic connecting pipe are connected to the gripping disc and the hollow meshing block. A top suction cup is fixed on the bottom surface of the gripping disc. A hole communicating with the inner side of the gripping disc is opened on the axis of the top suction cup. The end pipe head is located on the upper side of the cross frame. The upper side of the cross frame has a groove-shaped structure that runs vertically through it. The end pipe head is slidably inserted into the groove-shaped structure of the cross frame. The lower end of the end pipe head slides through the upper surface of the hollow meshing block. The end pipe head has an upper pressure relief hole at one end of the hollow meshing block. The end pipe head is elastically connected to the hollow meshing block. A linkage plate, one end of which is connected to the end of the end pipe located on the upper side of the cross frame; It also includes a central top rod, the lower end of which is eccentrically set with the central storage cylinder. When the gripping disc moves to be coaxial with the central storage cylinder, the axes of the linkage plate, the central top rod, and the central storage cylinder are located on the same axis.

[0013] Optionally, a linkage plate is provided on the lower side of the suspension plate, and the linkage plate is elastically connected to the suspension plate; The support plate has a hollow interior, and a hole communicating with the interior of the support plate is opened at the axis of the bottom suction cup. A pressure relief plug is slidably inserted into the lower end of the support plate. The lower end of the pressure relief plug is fixed to the upper surface of the linkage plate. A pressure relief hole is opened at one end of the pressure relief plug inside the support plate. A ring is provided between the linkage plate and the suspension plate. A vertical pole is fixed on the upper surface of the ring. The upper end of the central storage cylinder is slidably sleeved on the outer surface of the vertical pole. A double inclined panel is provided on the side of the central storage cylinder away from the vertical pole. The double inclined panel is fixed to the cross frame. The central storage cylinder is located between the double inclined panel and the gripping plate.

[0014] Optionally, an outer top rod is provided on one side of the cross frame. The outer top rod is vertically arranged, and the outer top rod and the central top rod are located on both sides of the cross frame, respectively. The outer top rod is fixed to the inner wall of the intelligent enclosed transfer box.

[0015] Optionally, both sides of the double-sloped panel are inclined surfaces, and the double-sloped panel is set as an arc-shaped plate structure coaxial with the central storage cylinder. As the double-sloped panel rotates around the axis of the central storage cylinder with the cross frame, it can push the upright to move downward.

[0016] In summary, the present invention has the following beneficial technical effects: In this invention, through the coordinated use of the chassis adsorption unit and the gripping and transfer unit, the gripping and transfer unit picks up the culture dish transferred from the internal and external transfer unit to the upper side of the chassis adsorption unit inside the intelligent closed transfer box, the chassis adsorption unit adsorbs the bottom of the culture dish, the gripping and transfer unit separates the top cover of the culture dish from the bottom of the culture dish, and at the same time, the suspension support assembly supports the culture dish in the air through the chassis adsorption unit, so that the culture dish can be fully placed in the internal environment of the intelligent closed transfer box.

[0017] This invention utilizes a transfer disc, a transfer trough, and a one-way gas extractor. A culture dish in a closed state is placed into the transfer trough through an opening. The transfer disc is then rotated to connect with the one-way gas extractor, which extracts any residual external air from the transfer trough. After the air is extracted, the transfer trough is moved into the intelligent closed transfer box by the transfer disc, preventing bacteria from the external air from entering the intelligent closed transfer box and reducing the contamination of cells inside the intelligent closed transfer box.

[0018] This invention utilizes a combination of pressure relief plugs, a lower pressure relief hole, an end tube head, and an upper pressure relief hole. When it is necessary to transfer the internal culture dish out, the bottom suction cup on the upper side of the support plate detaches from the culture dish. Then, the top suction cup at the bottom of the gripping plate is controlled to adhere to the top plate inside the central storage cylinder. The gripping plate is then driven to move to the top of the culture dish to be transferred, and the gripped top cover is re-interference-fitted with the culture dish. Then, during the upward movement, the fit between the top cover and the culture dish causes the entire assembly to detach from the bottom suction cup, transferring the culture dish to the corresponding transfer groove of the corresponding transfer plate, thus transferring the internal culture dish out. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure in an embodiment of the present invention; Figure 2 This is a schematic diagram of the internal structure of the intelligent enclosed transfer box in an embodiment of the present invention; Figure 3 This is a schematic diagram of the distribution of the suspension plate and the linkage plate in an embodiment of the present invention; Figure 4This is a schematic diagram of the internal structure of the central storage cylinder in an embodiment of the present invention; Figure 5 This is a schematic diagram of the structural distribution of the ring and the upright in an embodiment of the present invention; Figure 6 This is a schematic diagram of the grasping and transferring unit in an embodiment of the present invention; Figure 7 This is a schematic diagram of the structural distribution of the gripping disk and the hollow meshing block in an embodiment of the present invention; Figure 8 This is a schematic diagram of the distribution of the pressure relief plug and the support plate in an embodiment of the present invention.

[0020] Reference numerals: 1. Intelligent enclosed transfer box; 2. Internal and external transfer unit; 21. Transfer side box; 22. Placement opening; 23. One-way gas extractor; 24. Transfer tray; 25. Transfer trough; 3. Suspension support assembly; 31. Suspension tray; 32. Placement trough; 4. Chassis adsorption unit; 41. Support tray; 42. Bottom suction cup; 43. Linkage tray; 44. Pressure relief plug; 45. Lower pressure relief hole; 46. Ring; 47. Upright pole; 48. Double inclined panel; 5. Cover storage mechanism; 51. Central storage cylinder; 52. Tray; 6. Grasping and transfer unit; 61. Horizontal frame; 62. Hollow meshing block; 63. Threaded shaft; 64. Grasping tray; 65. Top suction cup; 66. End pipe head; 67. Upper pressure relief hole; 68. Linkage plate; 69. Central top rod; 610. Outer top rod; 611. Connecting pipe. Detailed Implementation

[0021] The following is in conjunction with the appendix Figures 1-8 The present invention will be described in further detail below.

[0022] This invention discloses a closed-loop docking and aseptic transport device for cell samples. For example... Figure 1 and Figure 2 As shown, it includes an intelligent enclosed transfer box 1, an internal and external transfer unit 2, a suspension support assembly 3, a chassis adsorption unit 4, a cover storage mechanism 5, and a gripping and transfer unit 6.

[0023] In this embodiment, the intelligent closed transport box 1 is provided with an observation window and an intelligent operation interface on its upper side. The intelligent closed transport box 1 is equipped with a temperature controller and a gas concentration sensor controller. The temperature controller can sense and change the internal temperature, and the gas concentration sensor controller can simultaneously detect the internal gas and control the internal gas concentration to make the internal space a suitable environment for cell culture.

[0024] There are two internal and external transport units 2. Both internal and external transport units 2 are installed inside the intelligent closed transport box 1. The internal and external transport units 2 can transfer the cell culture dishes on the outside to the inside. The culture dishes under the closed system can be placed into the internal and external transport units 2 and then transferred to the intelligent closed transport box 1.

[0025] The suspension support assembly 3 is coaxially installed inside the intelligent closed transfer box 1. The intelligent closed transfer box 1 can drive the suspension support assembly 3 to rotate along the axis. The chassis adsorption unit 4 is connected to the suspension support assembly 3. The adsorption end of the chassis adsorption unit 4 is set upward, and the chassis adsorption unit 4 adsorbs the culture dish placed on the upper side.

[0026] The cover storage mechanism 5 is installed at the axis of the intelligent closed transfer box 1. The suspension support assembly 3 is installed inside the cover storage mechanism 5. The suspension support assembly 3 rotates relative to the cover storage mechanism 5. The upper opening of the cover storage mechanism 5 extends out of the upper side of the suspension support assembly 3. The disassembled covers can be stored in the cover storage mechanism 5. Multiple covers are coaxially stacked in the cover storage mechanism 5.

[0027] The gripping and transfer unit 6 is installed inside the intelligent closed transfer box 1. The gripping and transfer unit 6 can transfer the culture dish in the inner and outer transfer unit 2 to the upper side of the chassis adsorption unit 4. After the chassis adsorption unit 4 adsorbs the bottom of the culture dish, the gripping and transfer unit 6 can separate the top cover of the culture dish from the bottom of the culture dish. The gripping and transfer unit 6 transfers the removed cover to the cover storage mechanism 5.

[0028] In this embodiment, the resistance generated by the interference fit between the upper and lower parts of the culture dish meets the following conditions: after the gripping and transfer unit 6 grips the upper end of the cover, the culture dish can be suspended and transferred as a whole under the resistance between the upper and lower parts of the culture dish. After the bottom adsorption unit 4 adsorbs the bottom of the culture dish, after the gripping and transfer unit 6 applies a pulling force to the cover again, the pulling force of the bottom adsorption unit 4 and the gripping and transfer unit 6 on the upper and lower parts of the culture dish can counteract the resistance between the two parts of the culture dish, and can separate the top cover of the culture dish from the culture dish, open the upper side of the culture dish, and expose the cells inside the culture dish to the culture environment inside the intelligent closed transport box 1, so that the cells are in a suitable culture environment during long-term transportation.

[0029] The internal and external transfer unit 2 includes a transfer side box 21, a one-way gas extractor 23, and a transfer tray 24.

[0030] The transfer side box 21 is installed through the circumference of the intelligent closed transfer box 1. The upper surface of the transfer side box 21 located on the outer part of the intelligent closed transfer box 1 has an insertion opening 22. The one-way gas extractor 23 is installed on the upper surface of the transfer side box 21 located on the outer part of the intelligent closed transfer box 1.

[0031] The transfer tray 24 is fitted inside the transfer side box 21. The intelligent closed transfer box 1 can drive the transfer tray 24 to rotate. The circumferential side of the transfer tray 24 has an upward-opening transfer groove 25. The one-way gas extractor 23 can extract the air inside the connected transfer groove 25. The distance between the transfer grooves 25 is sufficient so that the same transfer groove 25 will not be simultaneously connected to the insertion opening 22 and the one-way gas extractor 23 or the one-way gas extractor 23 and the interior of the intelligent closed transfer box 1.

[0032] In use, the petri dish in its closed state is placed into the corresponding connected transfer tank 25 through the insertion opening 22. As the drive transfer plate 24 rotates, the transfer tank 25 containing the petri dish is connected to the one-way gas extractor 23, which extracts the air from the transfer tank 25 to prevent external air from entering the intelligent closed transfer box 1 and causing pollution to the internal environment.

[0033] In this embodiment, as Figures 3-8 As shown, the suspension support assembly 3 includes a suspension plate 31, which is rotatably suspended inside the intelligent closed transfer box 1. The intelligent closed transfer box 1 drives the suspension plate 31 to rotate, and the suspension plate 31 is rotatably sleeved on the outside of the cover storage mechanism 5. Multiple placement slots 32 are opened on the upper surface of the suspension plate 31. The adsorption ends of the chassis adsorption unit 4 are distributed inside the multiple placement slots 32. The culture dish is placed in the placement slot 32 during transfer. The placement slot 32 limits the lateral movement range of the culture dish to prevent the culture dish from swinging around on the upper side of the suspension plate 31 when shaking occurs during transfer in the intelligent closed transfer box 1.

[0034] The chassis adsorption unit 4 includes a support plate 41. Multiple support plates 41 are provided and are installed one-to-one in multiple placement slots 32. A bottom suction cup 42 is fixed on the upper surface of the support plate 41.

[0035] The cover storage mechanism 5 includes a central storage cylinder 51. The lower end of the central storage cylinder 51 is installed at the center of the bottom of the intelligent closed transfer box 1. The upper end of the central storage cylinder 51 is open. The central storage cylinder 51 is located on the axis side of the suspension plate 31. A tray 52 is provided inside the central storage cylinder 51. The intelligent closed transfer box 1 drives the tray 52 to move up and down inside the central storage cylinder 51.

[0036] The intelligent closed transfer box 1 is equipped with a drive telescopic cylinder inside the central storage cylinder 51. After the cover is stored inside the central storage cylinder 51, the drive telescopic cylinder controls the height of the uppermost cover inside the central storage cylinder 51 by telescopic control, so that the height difference between the uppermost cover and the top of the central storage cylinder 51 is the height of one cover, which facilitates the gripping operation of the cover.

[0037] In this embodiment, the gripping and transfer unit 6 includes a crossbeam 61, a hollow meshing block 62, a gripping disc 64, an end tube head 66, a linkage plate 68, and a central push rod 69.

[0038] The crossbeam 61 is horizontally installed inside the intelligent enclosed transfer box 1. The crossbeam 61 is located on the upper side of the suspension plate 31. The intelligent enclosed transfer box 1 can drive the crossbeam 61 to move up and down. The intelligent enclosed transfer box 1 can drive the crossbeam 61 to rotate around the axis of the suspension plate 31. The hollow meshing block 62 is slidably inserted into the crossbeam 61. A threaded shaft 63 capable of power rotation is installed inside the crossbeam 61. The hollow meshing block 62 is threadedly sleeved on the outer surface of the threaded shaft 63. The crossbeam 61 is equipped with a servo motor that drives the threaded shaft 63 to rotate, controlling the rotation direction and speed of the threaded shaft 63, thereby controlling the moving direction, moving speed, and stopping position of the hollow meshing block 62.

[0039] The gripping disc 64 is located below the hollow meshing block 62. The gripping disc 64 and the hollow meshing block 62 are connected by a connecting pipe 611. Both ends of the connecting pipe 611 are connected to the gripping disc 64 and the hollow meshing block 62. A top suction cup 65 is fixed on the bottom surface of the gripping disc 64. The top suction cup 65 has a hole on its axis that communicates with the inside of the gripping disc 64.

[0040] When the top suction cup 65 adsorbs onto the top cover of the culture dish and the bottom suction cup 42 adsorbs onto the bottom of the culture dish simultaneously, as the top suction cup 65 moves up and down, the suction force of the top suction cup 65 and the bottom suction cup 42 is greater than the resistance between the cover and the bottom of the culture dish, which can separate the cover from the culture dish and allow the cells in the culture dish to come into contact with the culture environment inside the intelligent closed transport box 1.

[0041] The end tube head 66 is located on the upper side of the cross frame 61. The upper side of the cross frame 61 has a groove-shaped structure that runs vertically through it. The end tube head 66 is slidably inserted into the groove-shaped structure of the cross frame 61, which limits the range of movement of the end tube head 66. The lower end of the end tube head 66 slides through the upper surface of the hollow meshing block 62. The end tube head 66 has an upper pressure relief hole 67 at one end of the hollow meshing block 62. The end tube head 66 is elastically connected to the hollow meshing block 62. The elastic connection between the end tube head 66 and the hollow meshing block 62 is connected by a tension spring, which has a drive to pull the end tube head 66 into the hollow meshing block 62.

[0042] One end of the linkage plate 68 is connected to the end tube head 66 located on the upper side of the cross frame 61. The lower end of the central top rod 69 is eccentrically set with the central storage cylinder 51. When the grabbing disc 64 moves to be coaxial with the central storage cylinder 51, the axes of the linkage plate 68, the central top rod 69 and the central storage cylinder 51 are located on the same axis.

[0043] When the end of the crossbeam 61 away from the axis of the central storage cylinder 51 is located on the side of the transfer tray 24 on the placement side, when the gripping tray 64 moves to be coaxial with the central storage cylinder 51, the linkage plate 68 is located on the upper side of the central top rod 69. As the crossbeam 61 moves downward, the crossbeam 61 moves upward relative to the hollow meshing block 62 under the obstruction of the central top rod 69, so that the upper pressure relief hole 67 moves to the outside of the hollow meshing block 62, so that the top suction cup 65 disengages from the top cover and the top cover falls into the central storage cylinder 51 for stacked storage of multiple covers.

[0044] An outer top rod 610 is provided on one side of the cross frame 61. The outer top rod 610 is vertically arranged. The outer top rod 610 and the central top rod 69 are located on both sides of the cross frame 61, and the outer top rod 610 is fixed to the inner wall of the intelligent closed transfer box 1.

[0045] A linkage plate 43 is provided on the lower side of the suspension plate 31. The linkage plate 43 is elastically connected to the suspension plate 31 and the linkage plate 43 is elastically connected to the suspension plate 31 by a straight spring. When the linkage plate 43 is pulled close to the suspension plate 31, the support plate 41 is hollow inside. A hole communicating with the inside of the support plate 41 is opened at the axis of the bottom suction cup 42. A pressure relief plug 44 is slidably inserted into the lower end of the support plate 41. The lower end of the pressure relief plug 44 is fixed to the upper surface of the linkage plate 43. A pressure relief hole 45 is opened at one end of the pressure relief plug 44 inside the support plate 41. When the linkage plate 43 is at its minimum distance from the suspension plate 31, the pressure relief hole 45 is inside the support plate 41. When the bottom of the culture dish contacts the bottom suction cup 42, a sealed space is formed inside the support plate 41, and the bottom suction cup 42 can stably adsorb the culture dish.

[0046] A ring 46 is provided between the linkage plate 43 and the suspension plate 31. A vertical rod 47 is fixed on the upper surface of the ring 46. The upper end of the central storage cylinder 51 is slidably sleeved on the outer surface of the vertical rod 47. A double inclined panel 48 is provided on the side of the central storage cylinder 51 away from the vertical rod 47. The double inclined panel 48 is fixed to the cross frame 61. The central storage cylinder 51 is located between the double inclined panel 48 and the gripping plate 64.

[0047] Both sides of the double-sloped panel 48 are inclined surfaces. The double-sloped panel 48 is set as an arc-shaped plate structure coaxial with the central storage cylinder 51. As the double-sloped panel 48 rotates around the axis of the central storage cylinder 51 with the cross frame 61, it can push the upright 47 to move downward.

[0048] In use, when transferring external culture dishes into the intelligent closed transport box 1, the double-sloped panel 48 and the upright rod 47 are located on both sides of the central storage cylinder 51. When it is necessary to transfer the internal culture dishes to the outside of the intelligent closed transport box 1, the control frame 61 rotates half a turn relative to the central storage cylinder 51. During the rotation, the slope of the double-sloped panel 48 pushes the upright rod 47 downward, which pushes the linkage plate 43 downward through the ring 46, pulling the pressure relief plug 44 downward, disengaging the lower pressure relief hole 45 from the inside of the support plate 41, and the bottom suction cup 42 disengages from adsorbing the culture dish. At this time, when the gripping plate 64 moves to the coaxial position with the central storage cylinder 51, the linkage plate 68 moves downward with the horizontal frame 61 and does not contact the central top rod 69. The upper pressure relief hole 67 is positioned... Inside the hollow meshing block 62, when the cover contacts the top suction cup 65, a sealed space is formed inside the hollow meshing block 62. The top suction cup 65 can adsorb the contacting cover, pull the cover upward, and then move it to the culture dish to be transferred. After that, the cover is pressed against the culture dish again. When the horizontal frame 61 moves upward again, it pulls the entire culture dish upward and moves the culture dish to the upper side of the transfer groove 25 of the transfer tray 24. During the movement of the horizontal frame 61, the linkage plate 68 can contact the outer top rod 610. Under the obstruction of the outer top rod 610, the linkage plate 68 drives the upper pressure relief hole 67 to disengage from the hollow meshing block 62, so that the culture dish falls into the corresponding lower transfer groove 25. The culture dish is transferred out as the transfer tray 24 rotates.

[0049] In this embodiment, the intelligent enclosed transfer box 1 is equipped with a motor that drives the suspension plate 31 to rotate, a telescopic cylinder that controls the up and down movement of the cross frame 61 and a motor that drives the cross frame 61 to rotate around the axis of the central storage cylinder 51, and a motor that controls the rotation of the transfer plate 24.

[0050] In this invention, an air-tight structure is installed between the structures in the gas flow section to prevent gas and air pressure leakage, and bearings are adapted to be installed between the rotation of the structural axis to increase the smoothness of the movement and reduce wear.

[0051] The working principle is as follows: The internal and external transport unit 2 transfers the cell culture dish from the closed state on the outside to the inside. The intelligent closed transport box 1 drives the gripping and transfer unit 6 to grip the culture dish transferred from the internal and external transport unit 2 to the upper side of the chassis adsorption unit 4. At the same time, when placing multiple culture dishes, the intelligent closed transport box 1 drives the suspension support component 3 and the chassis adsorption unit 4 to rotate as a whole, which can place the culture dish at different positions of the suspension support component 3 and the chassis adsorption unit 4. The chassis adsorption unit 4 adsorbs the bottom of the culture dish. The gripping and transfer unit 6 separates the top cover of the culture dish from the bottom of the culture dish. At the same time, the suspension support component 3 supports the culture dish in the air through the chassis adsorption unit 4. The gripping and transfer unit 6 transfers the separated top cover of the culture dish to the cover storage mechanism 5. The culture dish transferred to the inside of the intelligent closed transport box 1 is opened and exposed to the culture environment inside the intelligent closed transport box 1.

[0052] The above are all preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A closed-loop docking and aseptic transport device for cell samples, comprising an intelligent closed transport box (1), characterized in that, Also includes: There are two internal and external transfer units (2). Both internal and external transfer units (2) are installed inside the intelligent closed transfer box (1). The internal and external transfer units (2) can transfer the cell culture dish on the outside to the inside. The suspension support assembly (3) is coaxially installed inside the intelligent enclosed transfer box (1), and the intelligent enclosed transfer box (1) can drive the suspension support assembly (3) to rotate along the axis. The chassis adsorption unit (4) is connected to the suspension support assembly (3). The adsorption end of the chassis adsorption unit (4) is set upward, and the chassis adsorption unit (4) adsorbs the culture dish placed on the upper side. The cover storage mechanism (5) is installed on the axis of the intelligent closed transfer box (1). The suspension support assembly (3) is installed inside the cover storage mechanism (5). The suspension support assembly (3) rotates relative to the cover storage mechanism (5). The upper opening of the cover storage mechanism (5) extends out of the upper side of the suspension support assembly (3). The gripping and transfer unit (6) is installed inside the intelligent closed transfer box (1). The gripping and transfer unit (6) can transfer the culture dish in the inner and outer transfer unit (2) to the upper side of the bottom plate adsorption unit (4). After the bottom plate adsorption unit (4) adsorbs the bottom of the culture dish, the gripping and transfer unit (6) can separate the top cover of the culture dish from the bottom of the culture dish. The gripping and transfer unit (6) transfers the removed cover to the inside of the cover storage mechanism (5).

2. The cell sample closed docking and aseptic transport device according to claim 1, characterized in that: The internal and external transfer unit (2) includes: The transfer side box (21) is installed through the circumference of the intelligent closed transfer box (1). The transfer side box (21) has an insertion opening (22) on the upper surface of the outer part of the intelligent closed transfer box (1). One-way gas extractor (23), which is installed on the upper surface of the outer part of the transfer side box (21) located in the intelligent closed transfer box (1); It also includes a transfer tray (24), which is fitted inside the transfer side box (21). The intelligent closed transfer box (1) can drive the transfer tray (24) to rotate. The transfer tray (24) has an upward-opening transfer groove (25) on its circumferential side. The one-way gas extractor (23) can extract the air inside the connected transfer groove (25).

3. The cell sample closed docking and aseptic transport device according to claim 1, characterized in that: The suspension support assembly (3) includes a suspension plate (31), which is suspended and rotated inside the intelligent closed transfer box (1). The intelligent closed transfer box (1) drives the suspension plate (31) to rotate. The suspension plate (31) rotates and is sleeved on the outside of the cover storage mechanism (5). Multiple placement slots (32) are opened on the upper surface of the suspension plate (31), and the adsorption ends of the chassis adsorption unit (4) are distributed inside the multiple placement slots (32).

4. The cell sample closed docking and aseptic transport device according to claim 3, characterized in that: The chassis adsorption unit (4) includes a support plate (41), and multiple support plates (41) are provided. The multiple support plates (41) are installed one-to-one in the interior of multiple placement slots (32). The upper surface of the support plate (41) is fixed with a bottom suction cup (42).

5. The cell sample closed docking and aseptic transport device according to claim 4, characterized in that: The cover storage mechanism (5) includes a central storage cylinder (51). The lower end of the central storage cylinder (51) is installed at the center of the bottom of the intelligent closed transfer box (1). The upper end of the central storage cylinder (51) is open. The central storage cylinder (51) is set on the axis side of the suspension plate (31). A tray (52) is set inside the central storage cylinder (51). The intelligent closed transfer box (1) drives the tray (52) to move up and down inside the central storage cylinder (51).

6. The cell sample closed docking and aseptic transport device according to claim 5, characterized in that: The grabbing and transferring unit (6) includes: A horizontal frame (61) is installed horizontally inside the intelligent enclosed transfer box (1). The horizontal frame (61) is set on the upper side of the suspension plate (31). The intelligent enclosed transfer box (1) can drive the horizontal frame (61) to move up and down. The intelligent enclosed transfer box (1) can drive the horizontal frame (61) to rotate around the axis of the suspension plate (31). Hollow meshing block (62), the hollow meshing block (62) is slidably inserted into the inside of the cross frame (61), and a threaded shaft (63) capable of power rotation is installed inside the cross frame (61), and the hollow meshing block (62) is threadedly sleeved on the outer surface of the threaded shaft (63); The gripping disc (64) is located below the hollow meshing block (62). The gripping disc (64) and the hollow meshing block (62) are connected by a connecting pipe (611). The two ends of the connecting pipe (611) are connected to the gripping disc (64) and the hollow meshing block (62). A top suction cup (65) is fixed on the bottom surface of the gripping disc (64). The top suction cup (65) has a hole on its axis that communicates with the inside of the gripping disc (64). End pipe head (66), the end pipe head (66) is located on the upper side of the cross frame (61), the upper side of the cross frame (61) has a groove structure that runs vertically through, the end pipe head (66) is slidably inserted into the groove structure of the cross frame (61), the lower end of the end pipe head (66) slides through the upper surface of the hollow meshing block (62), the end pipe head (66) is located at one end of the hollow meshing block (62) and has an upper pressure relief hole (67), the end pipe head (66) and the hollow meshing block (62) are elastically connected; Linkage plate (68), one end of which is connected to the end pipe head (66) located on the upper side of the cross frame (61); It also includes a central top rod (69), the lower end of which is eccentrically set with the central storage cylinder (51). When the grabbing disc (64) moves to be coaxial with the central storage cylinder (51), the axes of the linkage plate (68), the central top rod (69) and the central storage cylinder (51) are located on the same axis.

7. The cell sample closed docking and aseptic transport device according to claim 6, characterized in that: A linkage plate (43) is provided on the lower side of the suspension plate (31), and the linkage plate (43) is elastically connected to the suspension plate (31); The support plate (41) is hollow inside. A hole communicating with the inside of the support plate (41) is opened at the axis of the bottom suction cup (42). A pressure relief plug (44) is slidably inserted into the lower end of the support plate (41). The lower end of the pressure relief plug (44) is fixed to the upper surface of the linkage plate (43). A lower pressure relief hole (45) is opened at one end of the pressure relief plug (44) inside the support plate (41). A ring (46) is provided between the linkage plate (43) and the suspension plate (31). A vertical rod (47) is fixed on the upper surface of the ring (46). The upper end of the central storage cylinder (51) is slidably sleeved on the outer surface of the vertical rod (47). A double inclined panel (48) is provided on the side of the central storage cylinder (51) away from the vertical rod (47). The double inclined panel (48) is fixed to the cross frame (61). The central storage cylinder (51) is located between the double inclined panel (48) and the gripping plate (64).

8. The cell sample closed docking and aseptic transport device according to claim 6, characterized in that: An outer top rod (610) is provided on one side of the cross frame (61). The outer top rod (610) is vertically arranged. The outer top rod (610) and the central top rod (69) are located on both sides of the cross frame (61). The outer top rod (610) is fixed to the inner wall of the intelligent closed transfer box (1).

9. The cell sample closed docking and aseptic transport device according to claim 7, characterized in that: Both sides of the double-sloped panel (48) are inclined surfaces. The double-sloped panel (48) is set as an arc-shaped plate structure coaxial with the central storage cylinder (51). The double-sloped panel (48) can push the upright (47) to move downward as it rotates around the axis of the central storage cylinder (51) with the cross frame (61).

Images