Strain Pontibacter indicus HCB-X867 capable of producing haematochrome and application of strain Pontibacter indicus HCB-X867
By screening and optimizing the fermentation and extraction process of the Pontibacter indicus HCB-X867 strain, the problems of high cost and poor stability of microbial red pigment in textile dyeing were solved, achieving low-cost and high-efficiency red pigment dyeing effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JIANGSU HUACHENG BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-01-14
- Publication Date
- 2026-04-21
AI Technical Summary
Existing microbial red pigments for textile dyeing suffer from problems such as high fermentation costs, low yields, complex extraction processes, poor stability, and immature dyeing techniques, making it difficult to achieve large-scale application.
A new strain, Pontibacter indicus HCB-X867, was screened out. Red pigment was extracted by fermentation in a specific culture medium and by heat treatment and pH adjustment, which simplified the extraction process and made it suitable for dyeing various textiles.
It achieves low-cost fermentation and stable red pigment production, simplifies the extraction process, and is suitable for efficient dyeing of textiles such as silk, nylon, and polyester, which is in line with the trend of green manufacturing.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of microbial application technology, specifically relating to a red pigment-producing strain, Pontibacterindicus HCB-X867, and its applications. Background Technology
[0002] Natural pigments, due to their high safety, environmental friendliness, and natural colors, have broad application prospects in the food, cosmetics, pharmaceutical, and textile industries. In the textile industry, the use of natural pigments for dyeing aligns with green and sustainable development trends and has gradually become a research hotspot.
[0003] Currently, natural pigments mainly originate from plants, animals, and microorganisms. Plant pigments, such as madder root and turmeric, are limited by plant cultivation cycles, geographical location, and season, making large-scale, stable supply difficult. Animal pigments, such as carmine, have limited sources, complex extraction processes, and high costs, limiting their applications. In contrast, microbial pigments offer advantages such as short production cycles, controllable cultivation conditions, and freedom from natural environmental constraints, making them an important direction for natural pigment development.
[0004] Red, as one of the three primary colors, is widely used in textile dyeing. However, current research on microbial red pigments is still in its early stages. Large-scale production faces challenges such as high fermentation costs, low yields, complex extraction processes, poor stability, and immature dyeing techniques, limiting their application in textile dyeing. Therefore, isolating and screening a new strain that produces red pigment, has low fermentation costs, is stable, easy to extract, and suitable for textile dyeing is of great significance. Summary of the Invention
[0005] The purpose of this invention is to overcome the defects in the prior art and provide a red pigment-producing strain, Pontibacter indicus HCB-X867, and its application. It can produce red pigment, and the fermentation cost is low, the red pigment extraction process is simple, and the staining effect is good.
[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:
[0007] A red pigment-producing strain, Pontibacter indicus HCB-X867, is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35905.
[0008] A high-red pigment-producing medium for fermentation of the strain Pontibacter indicus HCB-X867 comprises the following raw materials at the following concentrations:
[0009] Yeast extract: 1-5 g / L, peptone: 5-25 g / L, glucose: 1-9 g / L, CaCl2: 1-5 g / L, MgSO4: 1-5 g / L, NaCl 10-20 g / L, K2HPO4: 1-5 g / L, pH 6.8-7.2. Yeast extract provides abundant complex nutrients, including amino acids, B vitamins, nucleotides, growth factors, and inorganic ions. Peptone provides nitrogen and amino acids, supporting cell growth and metabolism. Glucose, as an economical and effective carbon source, is rich in fermentable sugars and can effectively induce the synthesis of red pigments. Calcium chloride provides calcium ions, which help maintain cell membrane stability and intracellular enzyme activity. MgSO4 provides magnesium and sulfate ions. Magnesium ions are cofactors for many enzymes, participating in the synthesis and metabolism of nucleic acids, proteins, and carbohydrates, and are also important components of chlorophyll (if the strain contains chlorophyll) and cell membranes. K₂HPO₄, as a phosphate buffer system, maintains the pH stability of the culture medium, preventing drastic pH fluctuations caused by the accumulation of metabolic products during bacterial growth, which would inhibit growth. It also provides potassium and phosphate ions.
[0010] In one embodiment, a high-red pigment-producing medium for fermentation of the strain Pontibacter indicus HCB-X867 comprises the following raw materials at the following concentrations: yeast extract 4 g / L, peptone 15 g / L, glucose 6 g / L, CaCl2 1 g / L, MgSO4 3 g / L, NaCl 15 g / L, K2HPO4 1.5 g / L, with a pH of 6.8-7.0.
[0011] A fermentation culture method for the strain Pontibacter indicus HCB-X867 includes the following steps:
[0012] Step 1, Seed culture preparation: The activated strain Pontibacter indicus HCB-X867 was inoculated into LB liquid medium and cultured with shaking at 30 ℃ and 200-250 rpm until the OD600nm reached 1.8-2.2 (2.0±0.1) to obtain the seed culture;
[0013] Step 2: Inoculate the seed culture into the high-red pigment-producing medium and carry out fermentation culture to obtain the fermentation broth of strain Pontibacter indicusHCB-X867.
[0014] In one embodiment, the fermentation culture is carried out using shake flask culture, and the process conditions for shake flask culture are: shaking culture at 30°C and 200-250 rpm for 48-60 hours.
[0015] In one embodiment, the fermentation culture is carried out in a fermenter, and the process conditions for fermenter culture are as follows:
[0016] The initial fermentation conditions were set as follows: temperature 30 ℃, aeration rate 1 vvm, and stirring speed 300 rpm;
[0017] During fermentation, dissolved oxygen was controlled at 20%, and glucose was fed in. The glucose feed consisted of a 500 g / L glucose solution, and the final glucose concentration during fermentation was controlled to be ≥1 g / L.
[0018] The fermentation cycle is 48-60 hours.
[0019] A method for detecting red pigment in fermentation broth, employing spectrophotometry with a detection wavelength of 448 nm, involves pretreatment of the fermentation broth prior to detection. The pretreatment includes the following steps:
[0020] The fermentation broth of the strain Pontibacter indicus HCB-X867 was placed in a centrifuge tube and centrifuged at 11,000 r / min for 10 min at 4 ℃. After collecting the red supernatant, 3 times the volume of acetone was added, shaken well, and allowed to stand for 10 min. Then, it was centrifuged at 11,000 r / min for 10 min at 4 ℃ to obtain the red pigment sample for absorbance determination.
[0021] A method for extracting red pigment, comprising the following steps:
[0022] The fermentation broth of the strain *Pontibacter indicus* HCB-X867 was heated to 75-85 °C and kept at that temperature for 1-3 hours. After centrifugation, the supernatant was collected, and the pH of the supernatant was adjusted to 2.8-3.2 (pH 3.0). The mixture was then allowed to stand at 4 °C for at least 6 hours, followed by centrifugation again to obtain the supernatant. The pH was then adjusted to neutral to obtain the red pigment extract. This invention utilizes heat treatment to inactivate and disrupt the bacterial cells, promoting red pigment dispersion and increasing the total recovery rate. The heat-treated fermentation broth is then subjected to solid-liquid separation to remove bacterial fragments and other insoluble impurities. Further impurities are removed by adjusting the pH to obtain the red pigment extract. This method requires no organic solvents, is simple to operate, and environmentally friendly. The crude extract can be directly applied to staining applications or used as a raw material for further purification.
[0023] A method for dyeing textiles, wherein a red pigment extract obtained by the extraction method is used to dye textiles.
[0024] In one embodiment, the textile includes any one of silk, nylon, and polyester.
[0025] In one embodiment, the dyeing bath ratio is 1:10-50, and the dyeing temperature is 20-130 ℃.
[0026] The application of the strain Pontibacter indicus HCB-X867 or the red pigment extract in the production of red pigment or in textile dyeing.
[0027] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0028] This invention provides the first screening of a novel red pigment-producing strain, Pontibacterindicus HCB-X867, from wetland soil, which has the following advantages:
[0029] 1) It can produce red pigment, and the red pigment produced has good stability.
[0030] 2) Fermentation can be carried out using conventional culture media, yeast powder, glucose and other ingredients to produce red pigment. The fermentation cost is low and the components are clearly defined, making it suitable for large-scale fermentation.
[0031] 3) The red pigment it produces is easy to extract from the fermentation broth. The extraction process is simple, requires no organic solvents, and is environmentally friendly.
[0032] 4) It can be used for dyeing various textiles, such as silk, nylon, and polyester. It can be used not only with red pigment but also directly with crude red pigment extract. It has good dyeing effect, clean overall process, and sustainability, which is in line with the trend of green manufacturing. Attached Figure Description
[0033] Figure 1 This is a colony morphology diagram of strain Pontibacter indicus HCB-X867 on LB agar plates;
[0034] Figure 2 This is a sample image of the fermentation broth from strain Pontibacter indicus HCB-X867;
[0035] Figure 3 This is the phylogenetic tree of strain Pontibacter indicus HCB-X867;
[0036] Figure 4 This is a graph showing the change in red pigment production over time.
[0037] Figure 5 This is a diagram showing the effect of red pigment staining;
[0038] exist Figure 5 In the text, a: silk; b: nylon; c: polyester. Detailed Implementation
[0039] The present invention will be further described below with reference to the embodiments, but the present invention is not limited to the following embodiments.
[0040] 1. Strain HCB-X867, classified as Pontibacter indicus; deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO.35905, deposited on September 12, 2025; deposited at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.
[0041] 2. Unless otherwise specified, all raw materials used in this invention are commercially available.
[0042] Example 1: Isolation and Screening of Strains
[0043] (1) Sample collection: Soil samples were collected from the lakeside of Changguangxi Wetland Park in Wuxi City, Jiangsu Province using the five-point sampling method. Before sampling, the top 3-5 cm of loose soil was removed, and only soil samples from 5-10 cm were selected. The collected soil samples were then placed in sterilized self-sealing bags and sealed for preservation.
[0044] (2) Sample pretreatment: Weigh 10 g of the above soil sample on an electronic balance, place it in a sterile 250 mL conical flask, add 100 mL of sterile double-distilled water, and shake at 200 rpm for about 12 h.
[0045] (3) Isolation of red pigment-producing strains:
[0046] Remove the soil dilution from the incubator, and use a pipette to take 1 mL of the supernatant. Add this to a pre-prepared test tube containing 9 mL of sterile double-distilled water, and dilute stepwise. The final result will have a gradient of 10⁻⁶. -2 10 -3 10 -4 10 -5 10 -6 The diluted solution was prepared. 100 μL of the diluted solution was spread onto LB agar plates and incubated at 30 °C. Observations were taken every 12 h, 24 h, and 48 h, and corresponding experimental records were kept. Many bacterial strains were isolated from the diluted soil solution for the first time. Bright red strains were picked from the plates using a sterile inoculation loop.
[0047] (4) Purification and screening of strains: Select the red pigment-producing strains from step (3) and purify them on freshly prepared LB agar plates using the streak plate method. Incubate the plates at 30 ℃ for 24 h and observe the purity of the strains. Repeat this step until colonies are observed (e.g., ...). Figure 1As shown in the figure, the selected colonies were inoculated into fresh liquid culture medium for fermentation. Strains that could grow stably in the liquid culture medium and produce red pigment were screened, and one strain was obtained, which was denoted as strain HCB-X867.
[0048] (5) Preservation of strain: In a sterile clean bench, use a sterile inoculation loop to pick up the purified strain HCB-X867 and inoculate it into 3 mL of LB liquid medium. Incubate in a shaker at 30 °C until the logarithmic growth phase. Then take 1 mL of fermentation broth and 1 mL of 40% glycerol, mix well and label it. Store in a -80 °C refrigerator for later use.
[0049] Example 2: Identification of the strain
[0050] The strain preserved above was removed from a -80 ℃ freezer and inoculated into 3 mL of freshly prepared LB liquid medium. After incubation at 30 ℃ for 14 h, genomic DNA was extracted using a bacterial genomic DNA extraction kit. The 16S rDNA was amplified using universal primers 27F and 1492R and then sent to Suzhou Genewiz Biotechnology Co., Ltd. for sequencing. The obtained sequencing sequences were compared with the NCBI database using BLAST. From the comparison results, 16S rRNA sequences of other strains with high sequence characteristics were selected to obtain a phylogenetic relationship diagram, see [link to diagram]. Figure 3 ;
[0051] The nucleotide sequence of the 16S rDNA of strain HCB-X867 is shown in SEQ ID NO.1, and is as follows:
[0052] ATTTACCCTAGACGGCTCCTGTGACGGTCACCGGCTTCAGGTCTCCCTGACTTCCATGGCTTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGCCGTTGCTGATGCGCGATTACTAGCGATTCCGGCTTCACGGAGTCGAGTTGCAGACTCCGATCCGAACTGAGACCGGCTTTTTGAGATTGGCTCCCTGTCACCAGGTGGCAACCCTCTGTACCGGCCATTGTAGCACGTGTGTAGCCCTAGGCGTAAGGGCCATGATGACTTGACGTCGTCCCCGCCTTCCTCGCTTCTTGCGAAGGCAGTCCCTTTAGAGTCCCCGCCATGACGCGCTGGCAACTAAAGGTAGGGGTTGCGCTCGTTGCGGGACTTAACCCAACACCTCACGGCACGAGCTGACGACAGCCATGCAGCACCTTGCTTTGTGCCCCGAAGGGAAGGTCCATCTCTGGACCGGTCACGCGCATTCTAGCCTAGGTAAGGTTCCTCGCGTATCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCACCCTTGCGGGCGTACTCCCCAGGTGGATGACTTAACGCTTTCGCTTGGACGCTTGCCGTGTATCGCAAACATCGAGTCATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCGGCCCAGCGAGCTGCCTTCGCAATCGGGGTTCTTGGTGGTATCTATGCATTTCACCGCTACACCACCAGTTCCGCCCGCCTCGACCGAACTCAAGCCCGCCAGTATCAACGGCAGTTCCGGGGTTGAGCCCCGGGATTTCACCGCTGACTTAACGGGCCGCCTACGCACCCTTTAAACCCAATAAATCCNGNACAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGGAGTTAGCCCGTGCTTATTCGTACGGTACCGTCAG
[0053] After identification, strain HCB-X867 was found to belong to Pontibacter indicus, therefore, the strain was named Pontibacter indicus HCB-X867.
[0054] Example 3: Shake-flask fermentation, fed-batch fermentation, and determination of red pigment in Pontibacter indicus HCB-X867
[0055] 1. Culture medium
[0056] Seed culture medium: yeast extract 5.0 g / L, peptone 10.0 g / L, sodium chloride 10.0 g / L, agar 15.0 g / L, pH 7.0;
[0057] Fermentation medium: yeast extract 4 g / L, peptone 15 g / L, glucose 6 g / L, CaCl2 1 g / L, MgSO4 3 g / L, NaCl 15 g / L, K2HPO4 1.5 g / L, pH 7.0-7.2, culture temperature 30 ℃.
[0058] 2. Shake-flask fermentation
[0059] Single colonies were picked from agar plates and transferred to seed culture medium. The culture was maintained at 30 °C and 230 r / min for 12 h. Then, a 2% inoculum was transferred to fermentation medium and cultured at 30 °C and 230 r / min for 48 h to obtain the fermentation broth (e.g., ...). Figure 2 (As shown).
[0060] 3. Fermentation by feeding in batches
[0061] (1) Seed liquid preparation
[0062] Fresh single colonies of Pontibacter indicus HCB-X867 were picked and inoculated into Erlenmeyer flasks containing 50 mL of LB liquid medium. The flasks were shaken and cultured at 30 °C and 230 rpm for 12 h to obtain the primary seed culture.
[0063] (2) Preparation of secondary seed liquid
[0064] Inoculate the primary seed culture into the secondary seed culture medium at an inoculation rate of 2% (v / v) and culture at 30 ℃ and 230 rpm for 6-8 h. When the cell concentration OD600 reaches 2.0-2.5, use it as the fermentation inoculum.
[0065] (3) Fermentation by batch feeding
[0066] The secondary seed culture was inoculated into the fermentation medium in a 5 L fermenter at a 10% (v / v) inoculation rate. Initial fermentation conditions were set as follows: temperature 30 °C, aeration rate 1 vvm, and stirring speed 300 rpm. Changes in dissolved oxygen (DO) and stirring speed were monitored throughout the fermentation process, and feed was dynamically added based on cell growth rate and metabolic status. The feed consisted of 500 g / L glucose, with the final glucose concentration maintained at at least 1 g / L. Every 6 hours after fermentation began, samples were taken to measure the red pigment production. The total fermentation time was 60 hours.
[0067] (4) Detection of relative red pigment content in fermentation broth: During the fed-batch fermentation process, every 6 hours, 3 mL of fermentation broth sample was placed in a 5 mL centrifuge tube, shaken well, and centrifuged at 11000 r / min for 10 min at 4 ℃ to obtain red supernatant and cell precipitate; 3 times the volume of acetone solution was added to the red supernatant, shaken well, and allowed to stand for 10 min, then centrifuged at 11000 r / min for 10 min at 4 ℃. The supernatant was collected, and its absorbance was measured at a wavelength of 448 nm. The relative content of red pigment in cells was compared. The results are shown in […]. Figure 4 ;
[0068] from Figure 4 The results showed that after 54 h of fermentation in a 5 L fermentation system, the highest OD yield of red pigment was achieved by Pontibacter indicus HCB-X867. 448 nm The result reached 83.2%. This indicates that efficient red pigment biosynthesis was achieved through comprehensive optimization of the fermentation medium and fermentation method of the strain.
[0069] (5) Extraction of red pigment from fermentation broth
[0070] The Pontibacter indicus HCB-X867 fermentation broth, after fermentation for 54 h, was heated to 80 ℃ and kept at that temperature for 2 h. After centrifugation, the supernatant was collected, and the pH of the supernatant was adjusted to 3 or 4 ℃. The mixture was then allowed to stand for 6 h, and centrifuged again. The supernatant was then collected and the pH was adjusted to neutral to obtain the red pigment extract.
[0071] Example 4: Textiles
[0072] 1. Silk dyeing
[0073] (1) Silk pretreatment: Soak the silk in hot water, take it out and squeeze out the excess water, and use absorbent paper to absorb the surface moisture.
[0074] (2) Dyeing: The pH value of the red pigment extract obtained in Example 3 was adjusted to 5 as the dyeing solution. The silk pretreated in step (1) was soaked in the dyeing solution and dyed at room temperature of 20 °C for 60 min. Then it was taken out.
[0075] (3) Washing: Rinse the dyed silk in step (2) with clean water, then rinse with dilute acetic acid solution, prepare a 5 g / L soap washing solution with soap washing agent, bath ratio (g / ml) 1:50, and soap wash at room temperature for 5 min; rinse with clean water, hang to dry, and obtain silk products.
[0076] 2. Nylon dyeing
[0077] (1) Dyeing process: Soak nylon in the red pigment extract obtained in Example 3, heat to 80 ℃ and keep warm for 20 min, then take it out.
[0078] (2) Washing: The dyed nylon obtained in step (1) is rinsed with water and dilute acetic acid solution. A 5g / L soaping solution is prepared with soaping agent, with a bath ratio (g / ml) of 1:20. The nylon is soaped for 5 minutes at room temperature, rinsed with water, and dried to obtain the nylon product.
[0079] 3. Polyester dyeing
[0080] (1) Dyeing process: Soak the polyester in the red pigment extract obtained in Example 3, heat it to 130 ℃ and keep it warm for 25 min, then take it out.
[0081] (2) Washing: The dyed polyester obtained in step (1) is rinsed with water, and a 5g / L soaping solution is prepared with soaping agent. The bath ratio (g / ml) is 1:10. The polyester is soaped at 60 degrees Celsius for 10 minutes, rinsed with water, and hung to dry to obtain the polyester product.
[0082] 4. Staining results
[0083] The dyeing results of silk, nylon, and polyester in this embodiment are shown in the figure. Figure 5 ,like Figure 5 As shown, red pigment was successfully applied to silk, nylon, and polyester. This indicates that the red pigment obtained by fermentation of Pontibacter indicus HCB-X867 can be used not only for dyeing protein fibers such as silk, but also for dyeing chemical fibers such as polyester. Furthermore, the extract obtained by fermentation of Pontibacter indicus HCB-X867 can be directly used for dyeing textiles.
[0084] The embodiments described above are merely preferred embodiments of the present invention, and not an exhaustive list of all possible implementations of the present invention. Any obvious modifications made by those skilled in the art without departing from the principles and spirit of the present invention should be considered to be included within the scope of protection of the claims of the present invention.
Claims
1. A red pigment-producing strain, *Pontibacter indicus* HCB-X867, characterized in that... It is deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 35905.
2. A high-red pigment-producing medium for fermentation of the strain Pontibacter indicus HCB-X867 as described in claim 1, characterized in that, The raw materials include the following concentrations: yeast powder: 1-5 g / L, peptone: 5-25 g / L, glucose: 1-9 g / L, CaCl2: 1-5 g / L, MgSO4: 1-5 g / L, NaCl 10-20 g / L, K2HPO4: 1-5 g / L, and pH value of 6.8-7.
2.
3. A fermentation culture method for the strain Pontibacter indicus HCB-X867 as described in claim 1, characterized in that, Includes the following steps: Step 1, Seed culture preparation: The activated Pontibacter indicus HCB-X867 was inoculated into LB liquid medium and cultured with shaking at 30 ℃ and 200-250 rpm until the OD600nm reached 1.8-2.2 to obtain the seed culture; Step 2: Inoculate the seed culture into the high red pigment production medium described in claim 2 and carry out fermentation culture to obtain the fermentation broth of strain Pontibacter indicus HCB-X867.
4. The fermentation culture method for strain Pontibacter indicus HCB-X867 as described in claim 3, characterized in that, The fermentation culture was carried out in a shake flask, and the process conditions for the shake flask culture were: shaking culture at 30 ℃ and 200-250 rpm for 48-60 hours; Alternatively, the fermentation culture may be carried out in a fermenter, and the process conditions for fermenter culture are as follows: The initial fermentation conditions were set as follows: temperature 30 ℃, aeration rate 1 vvm, and stirring speed 300 rpm; During fermentation, dissolved oxygen was controlled at 20%, and glucose was fed in. The glucose feed consisted of a 500g / L glucose solution, and the final glucose concentration during fermentation was controlled to be ≥1g / L. The fermentation cycle is 48-60 hours.
5. A method for detecting red pigment in fermentation broth, characterized in that, The detection was performed using spectrophotometry at a wavelength of 448 nm. The fermentation broth was pretreated before detection, and the pretreatment included the following steps: Fermentation broth of the strain Pontibacter indicus HCB-X867 as described in claim 1 was placed in a centrifuge tube, centrifuged, and the red supernatant was collected. Three times the volume of acetone was added to the supernatant, the mixture was shaken well, allowed to stand, and then centrifuged to obtain the red pigment sample for absorbance determination.
6. A method for extracting red pigment, characterized in that, The process includes the following steps: heating the fermentation broth of the strain Pontibacter indicus HCB-X867 described in claim 1 to 75-85 ℃ and keeping it at that temperature for 1-3 h, centrifuging, collecting the supernatant, adjusting the pH of the supernatant to 2.8-3.2, letting it stand at 4 ℃ for at least 6 h, centrifuging again to obtain the supernatant, adjusting the pH to neutral, and obtaining the red pigment extract.
7. A method for dyeing textiles, characterized in that, The red pigment extract obtained by the extraction method described in claim 6 is used for dyeing textiles.
8. The method according to claim 7, characterized in that, The textiles include any one of silk, nylon, and polyester.
9. The method according to claim 7, characterized in that, The bath ratio for staining is 1:10-50, and the staining temperature is 20-130℃.
10. The use of the strain Pontibacter indicus HCB-X867 as described in claim 1 or the red pigment extract as described in claim 6 in the production of red pigment or in textile dyeing.