Preparation method and crushing method of fermentation liquor of raw materials

Abstract

The invention provides a raw material fermentation liquor preparation method and a crushing method, and belongs to the technical field of fermentation. Target raw materials are crushed to obtain raw material powder, and the raw material powder is soaked to obtain mixed liquor; adding a compound enzyme preparation into the mixed material liquid to carry out an enzymolysis reaction, and carrying out sterilization and enzyme deactivation treatment after the reaction is finished, so as to obtain a fermentation substrate; inoculating composite lactobacillus freeze-dried powder into the fermentation substrate for fermentation to obtain a fermentation product; the fermentation product is filtered and separated to obtain filtrate, and the filtrate is sterilized to obtain raw material fermentation liquor, so that sugar and macromolecular substances in the raw materials can be effectively converted while cell walls are broken to improve the extraction efficiency of functional components, the absorption rate is increased, and the application range is widened.

Description

Technical Field

[0001] This invention relates to the field of fermentation technology, and in particular to a method for preparing fermentation broth and a method for pulverizing raw materials. Background Technology

[0002] Food and medicinal herbs, such as ginseng and scutellaria, are widely used in the food, health product, and pharmaceutical industries due to their significant effects in regulating bodily functions, restoring physical weakness, and enhancing immunity. Traditional processing methods typically involve directly grinding the raw materials into fine powder, or slicing them and then soaking or decocting them to extract their nutritional and medicinal value.

[0003] Currently, conventional processing methods for medicinal and edible raw materials mainly rely on water as a medium for physical extraction or direct consumption. This traditional approach has significant limitations: First, simple water extraction or coarse processing is insufficient to effectively break down plant cell walls, leading to incomplete release of deeply encapsulated active ingredients, low extraction efficiency, and waste of raw material resources. Second, traditional methods cannot alter the chemical composition of the raw materials themselves; naturally occurring sugars such as starch and glucose are completely preserved, directly impacting the safety for blood sugar-sensitive individuals, such as diabetic patients. Furthermore, raw materials that have not undergone biotransformation are mostly macromolecules, making their absorption and efficacy by the human body highly uncertain.

[0004] Therefore, how to break down cell walls to improve the extraction efficiency of active ingredients while effectively converting sugars and macromolecules in raw materials has become a key issue that urgently needs to be addressed. Summary of the Invention

[0005] This invention provides a method for preparing fermentation broth and a pulverizing method for raw materials, which can break down cell walls to improve the extraction efficiency of active ingredients, while effectively converting sugars and macromolecules in the raw materials, thereby improving absorption rate and applicability.

[0006] A first aspect of the present invention provides a method for preparing fermentation broth of raw materials, comprising: The target raw material is crushed to obtain raw material powder, and the raw material powder is soaked to obtain a mixed liquid. A compound enzyme preparation is added to the mixture to carry out an enzymatic hydrolysis reaction, and after the reaction is completed, sterilization and enzyme inactivation treatment is performed to obtain the fermentation substrate; The compound Lactobacillus lyophilized powder was inoculated into the fermentation substrate for fermentation to obtain the fermentation product; The fermentation product is filtered and separated to obtain a filtrate, which is then sterilized to obtain the raw material fermentation broth.

[0007] Optionally, in one possible implementation of the first aspect, the soaking treatment of the raw material powder to obtain the mixed liquid includes: The raw material powder is soaked in water at 40-60℃ for 1-4 hours at a solid-liquid ratio of 1:8 to 1:15 to obtain a mixed liquid.

[0008] Optionally, in one possible implementation of the first aspect, the addition of a complex enzyme preparation to the mixed liquid for enzymatic hydrolysis, followed by sterilization and enzyme inactivation treatment after the reaction to obtain the fermentation substrate, includes: Add a compound enzyme preparation to the mixture and carry out an enzymatic hydrolysis reaction at 45-55°C for 1-4 hours to obtain the final solution after the reaction. After the reaction is complete, the liquid is heated to 85-95°C and maintained for 20-30 minutes to obtain the fermentation substrate; The compound enzyme preparation consists of cellulase, mesophilic amylase, pectinase and papain in a mass ratio of 2-5:1-2:1-3:1-3, and the amount of the compound enzyme preparation added is 1.0-3.0 wt% of the mass of the mixture.

[0009] Optionally, in one possible implementation of the first aspect, the step of inoculating the compound Lactobacillus lyophilized powder into the fermentation substrate for fermentation to obtain a fermentation product includes: A compound Lactobacillus lyophilized powder with an effective viable count concentration of 10⁹-10¹⁰ CFU / g was inoculated into the fermentation substrate and anaerobic fermented at 28-42℃ for 12-36 hours to convert the sugars and proteins in the fermentation substrate into bioactive substances and obtain the fermentation product. The liquid volume of the fermentation substrate was 60-85 wt% of the fermentation container volume. The compound lactobacillus freeze-dried powder is composed of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium infantis, and Lactobacillus rhamnosus in a preset mass ratio, which is any one of the following ratios: 4:1:2:3:2, 3:3:2:2:1, 3:1:3:3:2, 4:2:1:3:2, or 2:3:4:3:3. The inoculum amount of the compound lactobacillus freeze-dried powder is 0.5 to 1.0 wt% of the fermentation substrate.

[0010] Optionally, in one possible implementation of the first aspect, the step of filtering and separating the fermentation product to obtain a filtrate, and then sterilizing the filtrate to obtain a raw material fermentation broth, includes: The fermentation product is subjected to bag filtration to obtain filtrate, and the filtrate is sterilized at 65-75°C for 30-60 minutes to obtain raw material fermentation broth.

[0011] Optionally, in one possible implementation of the first aspect, the method for preparing the raw material fermentation broth according to claim 1 is characterized by comprising: S1, identify the attachments to the target raw material, obtain the attachment identification result, and compare the attachment identification result with a preset comparison table to determine the work unit; S2 controls the working unit in the multi-stage crushing device to crush the target raw material, obtain crushed material, and obtain the shape of the crushed material block; S3, when the shape of the material block is determined to be blocky, the crushed material is treated as blocky material, and the surface of the blocky material is identified to obtain the impurity identification result; S4, when the shape of the material block is determined to be a sheet shape, the crushed material is treated as sheet material, and the cross section of the sheet material is identified to obtain the impurity identification result; S5, when the shape of the material block is determined to be granular, the crushed material is treated as granular material, and the granular material is irradiated for identification to obtain the impurity identification result; S6, retrieve the stage comparison table of the working unit, compare the impurity identification result with the stage comparison table, determine the crushing unit of the corresponding crushing stage as the current working unit, and take the crushed material as the current target raw material. Repeat the above steps S2-S5 until the current working unit is the last crushing unit in the multi-stage crushing device, and obtain the raw material powder.

[0012] Optionally, in one possible implementation of the first aspect, the step of identifying the adhering substances on the target raw material to obtain an adhering substance identification result, and determining the work unit based on the adhering substance identification result and a preset comparison table, includes: Identify the target outline of the target raw material and calculate the area within the target outline to obtain the total area; The display area within the target outline is obtained based on the target pixel value of the target raw material, and the display area is calculated to obtain the target area; The attachment identification result is obtained based on the ratio of the target area to the total area; The preset interval in the preset comparison table where the attachment identification result is located is determined as the selected area, and the crushing unit corresponding to the selected area is retrieved as the working unit.

[0013] Optionally, in one possible implementation of the first aspect, the surface identification of the block material to obtain impurity identification results includes: Identify the surface of the block material to obtain block materials with fibrous parts and block materials with surface texture, and use the remaining block materials as re-inspection block materials; The re-inspected block material is subjected to a pressure test to obtain the pressure value of each re-inspected block material. The re-inspected block material whose pressure value is not within the preset pressure range is regarded as block impurity. The impurity identification result is obtained by the ratio of the number of blocky impurities to the total number of blocky materials. The process of cross-sectional identification of the sheet material to obtain impurity identification results includes: Acquire images of sheet material at the conveyor belt and identify the sheet-like outline of the sheet material in the acquired images; When a ring is identified within the sheet-like outline, the corresponding sheet-like material is taken as the material to be crushed, and the remaining sheet-like material is taken as sheet-like impurities. The impurity identification result is obtained by the ratio of the number of flaky impurities to the total number of flaky materials.

[0014] Optionally, in one possible implementation of the first aspect, the step of irradiating and identifying the particulate material to obtain impurity identification results includes: A bottom illumination image of the granular material is acquired, and impurity pixels in the bottom illumination image are identified based on a preset impurity pixel value range. The raw material area of ​​the granular material is obtained, and the area ratio of the impurity pixels in the raw material area is determined as the impurity identification result.

[0015] Optionally, in one possible implementation of the first aspect, it also includes: The flaky impurities and the blocky impurities are used as diffusion impurities; Obtain the crushing image of the current target raw material at the conveyor belt, and select the crushing stage corresponding to the current target raw material as the selected stage; Identify the outline of diffused impurities in the pulverized image as the diffusion outline, and retrieve the preset diffusion factor of the selected stage; The diffusion contour is magnified according to the preset diffusion factor to obtain the contour of interest in the shattered image; In response to the information indicating that the crushing of the selected stage is complete, the acquisition device is controlled to acquire the selected image of the crushed material at the corresponding conveyor belt of the selected stage; Based on the center points of the shattered image and the selected image, the shattered image and the selected image are aligned, and the contour of interest is updated into the selected image to obtain the region of interest within the selected image; Impurity identification is performed on the pulverized material within the area of ​​interest.

[0016] A second aspect of the present invention provides a storage medium storing a computer program, which, when executed by a processor, is used to implement the first aspect of the present invention and various methods possibly involved in the first aspect.

[0017] The beneficial effects of this invention are as follows: 1. This invention involves pulverizing, enzymatically hydrolyzing, and fermenting the target raw material to obtain a fermentation broth, whereas existing technologies often employ single extraction or direct fermentation methods. This method, by first pulverizing the raw material into powder and then subjecting it to enzymatic hydrolysis with a compound enzyme preparation, helps to disrupt the cell wall structure of the raw material, allowing for better release of its internal active ingredients. Subsequently, a compound lactobacillus is inoculated for fermentation, utilizing microbial metabolism to convert sugars and macromolecules in the raw material into easily absorbed small molecules. This improves the efficiency of component extraction while also enhancing the absorption rate and applicability of the raw material.

[0018] 2. This invention dynamically adjusts the grinding process based on impurity content. It matches the proportion of impurities in the raw material area to a preset stage comparison table. Based on the matching results, the system automatically decides whether to send the raw material to the next adjacent grinding unit for step-by-step processing, or skip intermediate steps and directly enter the next suitable grinding unit. This method of customizing the grinding path based on impurity levels can effectively remove impurities, eliminating corresponding impurities according to the characteristics of different stages.

[0019] 3. Based on the location of impurities and considering the disintegration characteristics of fragile impurities such as soil clods, the system identifies areas of interest. These areas can then be targeted for identification, thereby reducing the amount of data processing required. Attached Figure Description

[0020] Figure 1 A flowchart illustrating a method for preparing fermentation broth of a raw material provided by the present invention; Figure 2 A flowchart of a raw material pulverization method provided by the present invention; Figure 3 This is a schematic diagram of the contour of interest provided by the present invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] The terms "first," "second," "third," "fourth," etc. (if present) in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that embodiments of the invention described herein can be implemented in orders other than those illustrated or described herein.

[0023] It should be understood that in the various embodiments of the present invention, the sequence number of each process does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present invention.

[0024] It should be understood that in this invention, "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion, for example, a process, method, system, product, or device that includes a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to such process, method, product, or device.

[0025] It should be understood that in this invention, "multiple" refers to two or more. "And / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship. "Contains A, B, and C", "Contains A, B, and C" means that all three A, B, and C are contained; "Contains A, B, or C" means that one of A, B, and C is contained; "Contains A, B, and / or C" means that any one, two, or three of A, B, and C are contained.

[0026] It should be understood that in this invention, "B corresponding to A", "B corresponding to A", "A and B correspond", or "B and A correspond" means that B is associated with A, and B can be determined based on A. Determining B based on A does not mean determining B solely based on A; B can also be determined based on A and / or other information. Matching A and B is defined as a similarity between A and B that is greater than or equal to a preset threshold.

[0027] Depending on the context, "if" as used here can be interpreted as "when," "when," "in response to determination," or "in response to detection."

[0028] The technical solution of the present invention will be described in detail below with reference to specific embodiments. These specific embodiments can be combined with each other, and the same or similar concepts or processes may not be described again in some embodiments.

[0029] This invention provides a method for preparing fermentation broth of raw materials, such as... Figure 1 As shown, steps A1-A4 are included: A1. The target raw material is crushed to obtain raw material powder, and the raw material powder is soaked to obtain a mixed liquid.

[0030] In some embodiments, step A1 (soaking the raw material powder to obtain a mixed liquid) includes: The raw material powder is soaked in water at 40-60℃ for 1-4 hours at a solid-liquid ratio of 1:8 to 1:15 to obtain a mixed liquid.

[0031] It should be noted that after the raw materials are dried and pulverized, their cellular structure is in a shrunken state. If enzymatic hydrolysis is performed directly, the enzyme molecules will have difficulty penetrating into the tissue, resulting in low enzymatic hydrolysis efficiency. Furthermore, all addition ratios are by mass, i.e., the solid-liquid ratio of 1:8 to 1:15 is the corresponding mass ratio.

[0032] It is easy to understand that the qualified raw material powder, after being pulverized through multiple stages, is added to purified water at a volume of 8 to 15 times its weight. The water temperature is preheated and controlled between 40°C and 60°C. The stirring device is turned on to suspend the powder, and this temperature is maintained for constant-temperature soaking treatment, which lasts for 1 to 4 hours. After soaking, the resulting solid-liquid mixture is the mixed solution, which is then transferred to subsequent enzymatic hydrolysis.

[0033] On the one hand, by adding water, the powder particles are fully swelled and the intercellular gaps are increased, and the optimal activity of the subsequent complex enzyme is maintained by water at 40-60℃.

[0034] A2, add a compound enzyme preparation to the mixture to carry out enzymatic hydrolysis, and sterilize and inactivate the enzyme after the reaction to obtain the fermentation substrate.

[0035] In some embodiments, step A2 (adding a compound enzyme preparation to the mixed liquid for enzymatic hydrolysis, and sterilizing and inactivating the enzyme after the reaction to obtain the fermentation substrate) includes: Add a compound enzyme preparation to the mixture and carry out an enzymatic hydrolysis reaction at 45-55°C for 1-4 hours to obtain the final solution after the reaction.

[0036] After the reaction is complete, the liquid is heated to 85-95°C and maintained for 20-30 minutes to obtain the fermentation substrate.

[0037] The compound enzyme preparation consists of cellulase, mesophilic amylase, pectinase and papain in a mass ratio of 2-5:1-2:1-3:1-3, and the amount of the compound enzyme preparation added is 1.0-3.0 wt% of the mass of the mixture.

[0038] It should be noted that traditional water extraction processes are difficult to break down the tough cell walls of plants, resulting in a low extraction rate of deep-seated active ingredients. At the same time, the large molecules naturally present in the raw materials (such as starch and protein) are not easily absorbed directly by the human body, and the high sugar content limits the target population.

[0039] Therefore, a compound enzyme preparation is added to the mixed liquid, and the enzymatic hydrolysis reaction is carried out at 45-55℃ for 1-4 hours to obtain the liquid after the reaction. The liquid after the reaction is then heated to 85-95℃ and maintained for 20-30 minutes to obtain the fermentation substrate. The synergistic effect of cellulase and pectinase targets the main components of plant cell walls, such as cellulose and pectin, thoroughly disintegrating the cell structure and allowing for the full release of intracellular active substances. Mesophilic amylase converts the starch in the raw material into small molecule sugars, which not only reduces the glycemic index of the finished product but also provides the necessary carbon source for subsequent lactobacillus fermentation. Papain degrades large molecule proteins into peptides and amino acids, providing a nitrogen source and improving bioavailability. 45-55℃ is the optimal activity temperature range for the above-mentioned compound enzymes, and 1-4 hours ensures the thoroughness of the reaction. The high-temperature treatment at 85-95℃ after the reaction plays a dual role: it inactivates the enzymes, terminates the reaction, and prevents excessive hydrolysis, while also completing the basic sterilization before fermentation, creating a sterile environment for subsequent anaerobic fermentation.

[0040] A3. Inoculate the compound lactobacillus lyophilized powder into the fermentation substrate for fermentation to obtain the fermentation product.

[0041] In some embodiments, step A3 (inoculating the compound Lactobacillus lyophilized powder into the fermentation substrate for fermentation to obtain the fermentation product) includes: The compound Lactobacillus lyophilized powder with an effective viable count concentration of 10⁹-10¹⁰ CFU / g was inoculated into the fermentation substrate and anaerobic fermented at 28-42℃ for 12-36 h to convert the sugars and proteins in the fermentation substrate into bioactive substances and obtain the fermentation product. The volume of the fermentation substrate was 60-85 wt% of the fermentation container volume.

[0042] The compound lactobacillus freeze-dried powder is composed of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium infantis, and Lactobacillus rhamnosus in a preset mass ratio, which is any one of the following ratios: 4:1:2:3:2, 3:3:2:2:1, 3:1:3:3:2, 4:2:1:3:2, or 2:3:4:3:3. The inoculum amount of the compound lactobacillus freeze-dried powder is 0.5 to 1.0 wt% of the fermentation substrate.

[0043] It should be noted that while the substrate released its active ingredients after enzymatic hydrolysis, it still retains a large amount of natural sugars, such as glucose, fructose, and large molecular impurities. This limits its use by specific groups such as diabetics, and there is still room for improvement in taste and absorption rate. Single-strain fermentation often struggles to simultaneously address the needs of blood sugar reduction, aroma production, and functional factor generation.

[0044] Lactobacillus plantarum and Lactobacillus casei possess extremely strong acid-producing capabilities and environmental tolerance, rapidly lowering pH levels to inhibit contaminating bacteria. Lactobacillus bulgaricus and Lactobacillus rhamnosus contribute to the production of unique flavor compounds and extracellular polysaccharides. Bifidobacterium infantis, a core probiotic in the human gut, enhances the product's intestinal regulatory function. Through precise formulation, growth competition between strains is avoided, achieving symbiotic fermentation and maximizing fermentation efficiency. Simultaneously, under suitable temperatures of 28–42°C and anaerobic conditions, these lactic acid bacteria consume sugars in the substrate as a carbon source, converting them into beneficial organic acids such as lactic acid.

[0045] It should be noted that the fermentation strains used in the fermentation broth are Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium infantis, and Lactobacillus rhamnosus, with a freeze-dried powder concentration of 10⁹-10¹⁰ CFU / g.

[0046] Among them, Lactobacillus delbrueckii subsp. bulgaricus: China Industrial Microbial Culture Collection Center (CICC) strain: CICC 20353 Lactobacillus delbrueckii.

[0047] Lactobacillus plantarum subsp. plantarum: China Industrial Microbial Culture Collection Center (CICC) strain: CICC 20022 Lactobacillus plantarum subsp. plantarum.

[0048] Lactobacillus paracasei: China Industrial Microbial Culture Collection Center (CICC) strain: CICC 20241 Lactobacillus paracasei.

[0049] Bifidobacterium animalis subsp. lactis: China Industrial Microbial Culture Collection Center (CICC) strain: CICC 21709 Bifidobacterium animalis.

[0050] Lactobacillus rhamnosus: China Industrial Microbial Culture Collection Center (CICC) strain: CGMCC 1.2467 Lactobacillus rhamnosus.

[0051] A4. The fermentation product is filtered and separated to obtain a filtrate, and the filtrate is sterilized to obtain the raw material fermentation broth.

[0052] In some embodiments, step A4 (filtering and separating the fermentation product to obtain a filtrate, and sterilizing the filtrate to obtain a raw material fermentation broth) includes: The fermentation product is subjected to bag filtration to obtain filtrate, and the filtrate is sterilized at 65-75°C for 30-60 minutes to obtain raw material fermentation broth.

[0053] It should be noted that the product after fermentation is a complex mixture, which not only contains the bioactive substances we need, but also a large number of microorganisms (dead or live), incompletely degraded medicinal residues, and macromolecular flocculants.

[0054] Therefore, bag filtration, typically around 100 mesh, effectively intercepts suspended particles and most of the bacterial sludge, resulting in a clear and transparent filtrate. Low-temperature sterilization at 65-75℃, rather than high-temperature boiling, is chosen because many active ingredients produced during fermentation, such as specific small-molecule peptides, vitamins, and extracellular enzymes, are heat-sensitive. The 65-75℃ temperature range is sufficient to kill any remaining lactic acid bacteria, preventing further fermentation after bottling, which could lead to bottle explosion, souring, or the introduction of potential contaminants, ensuring safety. Simultaneously, it better preserves the bioactivity of heat-sensitive active ingredients, avoiding nutrient destruction caused by excessive heating.

[0055] It should be noted that during the crushing process, due to the different quality of different batches of raw materials, for example, some batches of ginseng are mixed with impurities, such as more soil lumps and metals, while some batches are mixed with less. In the existing technology, the same processing method is used for cleaning, cutting and filtering, which leads to different quality of the final raw material powder. Therefore, a multi-stage crushing device is set up to crush the raw materials in multiple stages. The crushing method proceeds from coarse crushing to obtain blocky raw materials, to finer crushing to obtain flaky raw materials, and finally to the finest granular raw material powder.

[0056] It is worth mentioning that the grinding stage is customized according to the amount of impurities in the raw materials. It is easy to understand that when the amount of impurities is small, it will be directly sent to the final and finest grinding method to be ground into raw material powder. When there are more impurities, it will be ground in stages and the impurities will be removed. When it is found that there are few impurities in a certain stage, it will still skip multiple intermediate grinding stages and go directly to the appropriate grinding unit for grinding.

[0057] Therefore, as Figure 2 The diagram shows a flowchart of a raw material pulverization method provided by the present invention. In some embodiments, the pulverization method applied in the preparation method of the raw material fermentation broth according to claim 1 includes S1-S6: S1, identify the attachments to the target raw material, obtain the attachment identification result, and compare the attachment identification result with a preset comparison table to determine the working unit.

[0058] In some embodiments, step S1 (identifying the adhering substances on the target raw material, obtaining the adhering identification result, and comparing the adhering identification result with a preset comparison table to determine the working unit) includes S11-S14: S11, identify the target outline of the target raw material, and calculate the area within the target outline to obtain the total area.

[0059] It should be noted that medicinal and edible raw materials, such as ginseng and scutellaria, are usually rhizomes. Freshly harvested or after initial washing, their surfaces often still have varying degrees of non-medicinal substances such as soil and sand attached. Current technology typically employs a uniform processing method, feeding all raw materials, regardless of their cleanliness, into the same grinder for identical impurity screening. The target raw material can be a root and tuber plant, such as ginseng or scutellaria, and the target outline is the outer outline of the target raw material, such as the outer outline of ginseng.

[0060] Furthermore, the area corresponding to all target raw materials is statistically analyzed to obtain the total area, for example, the total area corresponding to all ginseng in the collected image.

[0061] S12, obtain the display area within the target outline based on the target pixel value of the target raw material, and calculate the display area to obtain the target area.

[0062] The target pixel value can be a pre-set pixel value of the raw material's surface color, or a range of pixel values ​​determined based on the actual situation.

[0063] What is puzzling is that the exposed area of ​​the target raw material's surface is obtained and statistically analyzed to obtain the target area. It is worth mentioning that, as is not difficult to understand later, the larger the exposed area, the cleaner it is, and it is not blocked by soil clods or other impurities. Although some areas cannot be collected, the collected portions of multiple target raw materials from the same batch can be used to represent the cleanliness of this batch of target raw materials, and impurities will be removed in each subsequent crushing stage.

[0064] S13. Obtain the attachment identification result based on the ratio of the target area to the total area.

[0065] It's easy to understand that a larger adhesion recognition result indicates a cleaner product, while a smaller adhesion recognition result indicates more impurities.

[0066] S14, determine the preset interval in the preset comparison table where the attachment identification result is located as the selected area, and retrieve the crushing unit corresponding to the selected area as the working unit.

[0067] The preset comparison table has a one-to-one correspondence between preset areas and crushing units. For example, 0.9-1 indicates that the cleaner one corresponds to the last crushing unit in the multi-stage crushing device, which directly crushes the target raw material into raw material powder.

[0068] It is easy to understand that the corresponding crushing unit will be determined based on the cleanliness of the target raw material for customized crushing. It should also be noted that before crushing, vibration and other methods will be used to shake off the soil and other debris covering the target raw material. Afterwards, the crushed target raw material will be spread out using a sweeping device or mechanical material spreading to facilitate subsequent identification.

[0069] S2 controls the working unit in the multi-stage crushing device to crush the target raw material, obtain crushed material, and acquire the shape of the crushed material block.

[0070] Among them, the multi-stage crushing device is a device that performs multiple crushing operations. The crushing method can be from coarse crushing to obtain blocky raw materials, to finer crushing to obtain flaky raw materials, and then to the finest granular raw material powder. The working unit refers to the crushing unit that is currently activated in the multi-stage crushing device, which can be a coarse crusher, a slicer, or an ultra-micro crusher, etc.

[0071] S3, when the shape of the material block is determined to be blocky, the crushed material is treated as blocky material, and the surface of the blocky material is identified to obtain the impurity identification result.

[0072] In some embodiments, step S3 (performing surface identification on the block material to obtain impurity identification results) includes S31-S33: S31, identify the surface of the block material, obtain the block material with fibrous parts and the block material with surface texture, and take the remaining block material as the re-inspection block material.

[0073] It should be noted that for lumpy materials in the coarse crushing stage, such as ginseng, there are obvious biological appearance characteristics. For example, the lateral roots or rhizome of ginseng usually have slender fibrous roots, which is the most intuitive feature that distinguishes it from soil clods or stones. The main root usually has unique iron wire patterns or transverse growth textures, while the surface of soil clods is usually a messy accumulation of particles or a smooth fracture surface.

[0074] However, existing technologies cannot perform targeted screening; they only filter the material through screens and then break it into powder.

[0075] Therefore, the surface of the block material will be identified, and block materials with fibrous parts and block materials with surface texture will be obtained, while the remaining block materials will be used as re-inspection block materials.

[0076] S32, perform a pressure test on the re-inspected block material to obtain the pressure value of each re-inspected block material, and regard the re-inspected block material whose pressure value is not within the preset pressure range as block impurities.

[0077] It should be noted that for medicinal plants, such as ginseng and astragalus, there are cases where they have neither growth lines nor fibrous parts. In such cases, it is difficult to distinguish whether they are impurities or the target raw material. Impurities are generally soil clods, stones, etc. Soil clods are usually loose in texture and will break easily when pressed, resulting in a sudden drop in pressure value; stones are hard in texture, almost immutable, and have extremely high pressure value, but medicinal plants are elastic.

[0078] The preset pressure range can be a standard numerical range that is pre-set by humans based on the target raw material.

[0079] Therefore, a device with a pressure sensor, such as a mechanical hand, will press the re-inspection block material with a fixed force. This force can be preset according to the different target raw materials to obtain the pressing value of each re-inspection block material. Re-inspection block materials whose pressing value is not within the preset pressure range are regarded as block impurities.

[0080] It is easy to understand that the lumpy material with extremely high pressure value and the sudden drop in pressure value that breaks easily under pressure is regarded as lumpy impurities, and the impurities can be removed in the future based on their location.

[0081] S33. The impurity identification result is obtained based on the ratio of the number of blocky impurities to the total number of blocky materials.

[0082] S4, when the shape of the material block is determined to be a sheet shape, the crushed material is treated as sheet material, and the cross-section of the sheet material is identified to obtain the impurity identification result.

[0083] In some embodiments, step S4 (performing cross-sectional identification of the sheet material to obtain impurity identification results) includes: S41, acquire an image of the sheet material at the conveyor belt, and identify the sheet-like outline of the sheet material in the acquired image.

[0084] It should be noted that when raw materials undergo secondary crushing, such as slicing, the cut surfaces of common medicinal plants, such as ginseng and astragalus, have circular ring patterns, such as cambium ring patterns.

[0085] Therefore, images of the sheet material at the conveyor belt will be acquired, and the sheet-like outline of the sheet material in the acquired images will be identified.

[0086] S42, when the disc-shaped outline is identified to have a ring, the corresponding disc-shaped material is taken as the material to be crushed, and the remaining disc-shaped material is taken as disc-shaped impurities.

[0087] It's easy to understand that when all the material is in sheet form, the cross-section of soil and stone after slicing usually has irregular textures or uniform particles and does not have circular rings. Therefore, the remaining sheet material is regarded as sheet impurities and will be removed later according to the location of the impurities, such as by using a suction head.

[0088] S43. The impurity identification result is obtained based on the ratio of the number of flaky impurities to the total number of flaky materials.

[0089] In addition to the above, it also includes B1-B7: B1, the flaky impurities and the blocky impurities are regarded as diffusion impurities.

[0090] It should be noted that when impurities, such as clods of soil or stones, are detected in previous steps and removed through screening, the removal is often mechanical, such as by blowing them off with an air gun or using a paddle to remove them. For fragile impurities like clods of soil, mechanical removal or vibration during the conveying process can easily cause them to disintegrate. These impurities will enter the next stage of crushing along with the raw material. Under physical impact, the impurities will not disappear into thin air, but will break and splash in their original positions.

[0091] B2, acquire the crushing image of the current target raw material at the conveyor belt, and select the crushing stage corresponding to the current target raw material as the selected stage.

[0092] It is understandable that the vision acquisition device located above the conveyor belt is triggered to capture an image of the current target raw material at the conveyor belt as a crushing image, and the crushing stage corresponding to the current target raw material is selected as the stage. Each crushing stage has a preset diffusion multiple according to its fineness of crushing, which can be preset by humans according to the actual situation. It is not difficult to understand that the finer the crushing, the larger the range of impurity disintegration.

[0093] B3, identify the contour of diffused impurities in the pulverized image as the diffusion contour, and retrieve the preset diffusion multiple of the selected stage.

[0094] B4. The diffusion contour is magnified according to the preset diffusion factor to obtain the contour of interest in the shattered image.

[0095] It is easy to understand that, in the current crushing stage, the collected impurity contours are magnified to obtain the contours of interest. These contours of interest are then mapped onto the image collected after the next crushing unit has finished crushing. (See [link to relevant documentation]). Figure 3 The diffusion contours in the shattered image are magnified to obtain the contours of interest after they can be shattered.

[0096] B5, responding to the information that the crushing is completed in the selected stage, controls the acquisition device to acquire the selected image of the crushed material at the corresponding conveyor belt in the selected stage.

[0097] It is easy to understand that the selected stage is determined by the number of impurities in the crushing image, that is, by the impurity identification result. If there are many impurities, it will enter the next crushing stage. If there are few impurities, it can skip multiple intermediate crushing stages and go to the crushing unit corresponding to the appropriate crushing stage.

[0098] Therefore, when the crushing unit of the selected stage finishes crushing the current target raw material, it generates crushing completion information for the selected stage. In response to this information, it controls the acquisition device to acquire the selected image of the crushed material at the corresponding conveyor belt of the selected stage.

[0099] B6, based on the center point of the shattered image and the selected image, aligns the shattered image and the selected image, updates the focus contour to the selected image, and obtains the focus area within the selected image.

[0100] It is worth mentioning that the drive belts of each crushing unit in the multi-stage crushing device are identical, and cameras are set at the same position in each unit. This facilitates the subsequent image alignment processing, updating the image after the next crushing stage to include the location where impurities appeared and spread out during the previous crushing stage. This allows for targeted detection of the location in the image.

[0101] Therefore, based on the center points of the shattered image and the selected image, the shattered image and the selected image can be aligned, thereby updating the contour of interest into the selected image and obtaining the region of interest within the selected image.

[0102] B7 identifies impurities in the crushed material within the area of ​​interest.

[0103] It is easy to understand that subsequent impurity identification can be performed on the region of interest, thereby reducing the amount of data processing.

[0104] S5, when the shape of the material block is determined to be granular, the crushed material is treated as granular material, and the granular material is irradiated for identification to obtain the impurity identification result.

[0105] In some embodiments, step S5 (irradiating the particulate material for identification to obtain impurity identification results) includes S51-S52: S51, acquire the bottom illumination image of the granular material, and identify the impurity pixels in the bottom illumination image based on the preset impurity pixel value range.

[0106] It should be noted that when the raw materials are pulverized into fine particles, the previous surface textures, such as iron wire patterns and cross-sectional features, such as growth rings, completely disappear, and the particles are too small to be physically pressed. At this point, the distinguishing factor between plant tissues and soil, stones, etc., is light transmittance. Plant tissues that are both medicinal and edible, such as ginseng granules, are usually composed of cellulose and are translucent, exhibiting a bright color under strong light; while inorganic impurities such as soil clods and sand are completely opaque. Therefore, when the granules are in powder form, a light source below the light-transmitting conveyor belt is used for irradiation.

[0107] The preset impurity pixel value range can be a pixel value area that is set in advance by the user. For example, it can be an opaque pixel value range, which is used to identify opaque objects later.

[0108] Therefore, the upper acquisition device will be controlled to acquire images of the granular material at the conveyor belt as a bottom illumination map, and the impurity pixels in the bottom illumination map will be identified based on the preset impurity pixel value range.

[0109] S52, obtain the raw material area of ​​the granular material, and determine the area ratio of the impurity pixel in the raw material area as the impurity identification result.

[0110] The raw material area refers to the area in the image covered by granular material.

[0111] It is understandable that the impurity identification result is based on the proportion of impurity pixels in the raw material area. That is, the proportion of impurities in the raw material area is used as the impurity identification result.

[0112] S6, retrieve the stage comparison table of the working unit, compare the impurity identification result with the stage comparison table, determine the crushing unit of the corresponding crushing stage as the current working unit, and take the crushed material as the current target raw material. Repeat the above steps S2-S5 until the current working unit is the last crushing unit in the multi-stage crushing device, and obtain the raw material powder.

[0113] It should be noted that the subsequent crushing unit will be customized based on the impurity identification results. If there are many impurities, the target raw material will be crushed step by step. If there are few impurities, the intermediate crushing unit will be skipped and the material will be crushed in the subsequent suitable crushing unit.

[0114] Each crushing unit has a pre-configured stage comparison table. This stage comparison table has a one-to-one correspondence between preset impurity percentage ranges and subsequent corresponding crushing stages. Each crushing stage has a corresponding crushing unit. When checking which preset impurity percentage range the impurity identification result falls into, the crushing unit of the crushing stage corresponding to the impurity percentage range is retrieved as the current working unit, and the crushed material is taken as the current target raw material. Steps S2-S5 are repeated to crush and identify impurities again. The crushing unit is determined again based on the impurity percentage, and crushing is carried out until the current working unit is the last crushing unit in the multi-stage crushing device, and the raw material powder is obtained.

[0115] By using the above methods, not only can impurities be reduced, but raw materials of the corresponding shapes can also be obtained directly at different stages.

[0116] The present invention also provides a readable storage medium storing a computer program, which, when executed by a processor, is used to implement the methods provided in the various embodiments described above.

[0117] The readable storage medium can be a computer storage medium or a communication medium. A communication medium includes any medium that facilitates the transfer of computer programs from one location to another. A computer storage medium can be any available medium accessible to a general-purpose or special-purpose computer. For example, a readable storage medium is coupled to a processor, enabling the processor to read information from and write information to the readable storage medium. Of course, the readable storage medium can also be a component of the processor. The processor and the readable storage medium can reside in an Application-Specific Integrated Circuit (ASIC). Alternatively, the ASIC can be located in a user equipment. Of course, the processor and the readable storage medium can also exist as discrete components in a communication device. The readable storage medium can be a read-only memory (ROM), random access memory (RAM), CD-ROM, magnetic tape, floppy disk, and optical data storage device, etc.

[0118] The present invention also provides a program product including executable instructions stored in a readable storage medium. At least one processor of the device can read the executable instructions from the readable storage medium, and the at least one processor executes the executable instructions to cause the device to implement the methods provided in the various embodiments described above.

[0119] In the embodiments of the above-described device, it should be understood that the processor can be a Central Processing Unit (CPU), or other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), etc. The general-purpose processor can be a microprocessor or any conventional processor. The steps of the method disclosed in this invention can be directly manifested as execution by a hardware processor, or execution by a combination of hardware and software modules within the processor.

[0120] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing fermentation broth from a raw material, characterized in that, include: The target raw material is crushed to obtain raw material powder, and the raw material powder is soaked to obtain a mixed liquid. A compound enzyme preparation is added to the mixture to carry out an enzymatic hydrolysis reaction, and after the reaction is completed, sterilization and enzyme inactivation treatment is performed to obtain the fermentation substrate; The compound Lactobacillus lyophilized powder was inoculated into the fermentation substrate for fermentation to obtain the fermentation product; The fermentation product is filtered and separated to obtain a filtrate, which is then sterilized to obtain the raw material fermentation broth.

2. The method according to claim 1, characterized in that, The process of soaking the raw material powder to obtain a mixed liquid includes: The raw material powder is soaked in water at 40-60℃ for 1-4 hours at a solid-liquid ratio of 1:8 to 1:15 to obtain a mixed liquid.

3. The method according to claim 1, characterized in that, The process involves adding a compound enzyme preparation to the mixed liquid for enzymatic hydrolysis, followed by sterilization and enzyme inactivation treatment after the reaction to obtain a fermentation substrate, comprising: Add a compound enzyme preparation to the mixture and carry out an enzymatic hydrolysis reaction at 45-55°C for 1-4 hours to obtain the final solution after the reaction. After the reaction is complete, the liquid is heated to 85-95°C and maintained for 20-30 minutes to obtain the fermentation substrate; The compound enzyme preparation consists of cellulase, mesophilic amylase, pectinase and papain in a mass ratio of 2-5:1-2:1-3:1-3, and the amount of the compound enzyme preparation added is 1.0-3.0 wt% of the mass of the mixture.

4. The method according to claim 1, characterized in that, The step of inoculating the compound lactobacillus lyophilized powder into the fermentation substrate for fermentation to obtain the fermentation product includes: The effective viable bacteria concentration is 10. 9 -10 10 The CFU / g compound Lactobacillus lyophilized powder was inoculated into the fermentation substrate and anaerobic fermented at 28-42℃ for 12-36h to convert the sugars and proteins in the fermentation substrate into bioactive substances and obtain the fermentation product. The liquid volume of the fermentation substrate was 60-85wt% of the fermentation container volume. The compound lactobacillus freeze-dried powder is composed of Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium infantis, and Lactobacillus rhamnosus in a preset mass ratio, which is any one of the following ratios: 4:1:2:3:2, 3:3:2:2:1, 3:1:3:3:2, 4:2:1:3:2, or 2:3:4:3:

3. The inoculum amount of the compound lactobacillus freeze-dried powder is 0.5 to 1.0 wt% of the fermentation substrate.

5. The method according to claim 1, characterized in that, The fermentation product is filtered and separated to obtain a filtrate, and the filtrate is then sterilized to obtain a raw material fermentation broth, comprising: The fermentation product is subjected to bag filtration to obtain filtrate, and the filtrate is sterilized at 65-75°C for 30-60 minutes to obtain raw material fermentation broth.

6. A raw material pulverization method, applied to the preparation method of the raw material fermentation broth according to claim 1, characterized in that, include: S1, identify the attachments to the target raw material, obtain the attachment identification result, and compare the attachment identification result with a preset comparison table to determine the work unit; S2 controls the working unit in the multi-stage crushing device to crush the target raw material, obtain crushed material, and obtain the shape of the crushed material block; S3, when the shape of the material block is determined to be blocky, the crushed material is treated as blocky material, and the surface of the blocky material is identified to obtain the impurity identification result; S4, when the shape of the material block is determined to be a sheet shape, the crushed material is treated as sheet material, and the cross section of the sheet material is identified to obtain the impurity identification result; S5, when the shape of the material block is determined to be granular, the crushed material is treated as granular material, and the granular material is irradiated for identification to obtain the impurity identification result; S6, retrieve the stage comparison table of the working unit, compare the impurity identification result with the stage comparison table, determine the crushing unit of the corresponding crushing stage as the current working unit, and take the crushed material as the current target raw material. Repeat the above steps S2-S5 until the current working unit is the last crushing unit in the multi-stage crushing device, and obtain the raw material powder.

7. The method according to claim 1, characterized in that, The process of identifying adhering substances on the target raw material, obtaining adhering substance identification results, and comparing these results with a preset comparison table to determine the work unit includes: Identify the target outline of the target raw material and calculate the area within the target outline to obtain the total area; The display area within the target outline is obtained based on the target pixel value of the target raw material, and the display area is calculated to obtain the target area; The attachment identification result is obtained based on the ratio of the target area to the total area; The preset interval in the preset comparison table where the attachment identification result is located is determined as the selected area, and the crushing unit corresponding to the selected area is retrieved as the working unit.

8. The method according to claim 1, characterized in that, The surface identification of the block material to obtain impurity identification results includes: Identify the surface of the block material to obtain block materials with fibrous parts and block materials with surface texture, and use the remaining block materials as re-inspection block materials; The re-inspected block material is subjected to a pressure test to obtain the pressure value of each re-inspected block material. The re-inspected block material whose pressure value is not within the preset pressure range is regarded as block impurity. The impurity identification result is obtained by the ratio of the number of blocky impurities to the total number of blocky materials. The process of cross-sectional identification of the sheet material to obtain impurity identification results includes: Acquire images of sheet material at the conveyor belt and identify the sheet-like outline of the sheet material in the acquired images; When a ring is identified within the sheet-like outline, the corresponding sheet-like material is taken as the material to be crushed, and the remaining sheet-like material is taken as sheet-like impurities. The impurity identification result is obtained by the ratio of the number of flaky impurities to the total number of flaky materials.

9. The method according to claim 1, characterized in that, The step of irradiating and identifying the granular material to obtain impurity identification results includes: A bottom illumination image of the granular material is acquired, and impurity pixels in the bottom illumination image are identified based on a preset impurity pixel value range. The raw material area of ​​the granular material is obtained, and the area ratio of the impurity pixels in the raw material area is determined as the impurity identification result.

10. The method according to claim 8, characterized in that, Also includes: The flaky impurities and the blocky impurities are used as diffusion impurities; Obtain the crushing image of the current target raw material at the conveyor belt, and select the crushing stage corresponding to the current target raw material as the selected stage; Identify the outline of diffused impurities in the pulverized image as the diffusion outline, and retrieve the preset diffusion factor of the selected stage; The diffusion contour is magnified according to the preset diffusion factor to obtain the contour of interest in the shattered image; In response to the information indicating that the crushing of the selected stage is complete, the acquisition device is controlled to acquire the selected image of the crushed material at the corresponding conveyor belt of the selected stage; Based on the center points of the shattered image and the selected image, the shattered image and the selected image are aligned, and the contour of interest is updated into the selected image to obtain the region of interest within the selected image; Impurity identification is performed on the pulverized material within the area of ​​interest.

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